Carg06208 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg06208
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionenoyl-CoA delta isomerase 2, peroxisomal-like
LocationCarg_Chr06: 8514513 .. 8515532 (-)
RNA-Seq ExpressionCarg06208
SyntenyCarg06208
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGCACACTTGAGAGGCGTGGCGATCTCTTCATCATCACCCTCACCGGCGACGACGAGCACTGGATCGGACCACCATTCATTTCCTCCTTCCTCTCTCTTCTGACCCAAATCAACTCCCAAGCCACGCCCGGTTCCGTTCTCATCACCACCTCCCATGGCCGCTTCTTCTCCAATGGCTTCCACCTTCCCTGGGTTCAATCCGCCGCTAGAGACCGCATACTCCACATGGTTCATATCTTCAAGCCTGTCGTCGCCGCCCTACTCTCTCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCATGCCGCCGCCGCCGGTTTTGCTCTCGCTCTTAGCCATGATTACGTCCTGATGAGGCGCGATAAAGGCGTGCTCTACATGAGCGAGGTCAGTTAACTAAATCATAATTCATAAACTTTTTTATACAAATAAACGTAATATTTATTTTAATCCAAATATGTTGTAAAAATTCTTCAAATAAACTAAATGCAAAATAAGATTTAAAGGTAATATGACCATAAGTTTTTTTTTTTTTAAATAAAAAATTTACAATAATTTGAAGTGATAATATCTTGACCCAATGGACGTTTATTAATTCTAATAACAAAGTAATTTATCATTCATAAACACATTTATATAAATCAAACAATTAAAAATTGAGAATAAAATATCTAATTATGGCGAGGAATAAAACTTGTAATGTATTATTCATGACAGATTGACTTGGGTTTTACGTTGCCCGATTATTTTGCGGCATTGATGAGGTCAAAGATCGGGTCAGCCTCAGTTCGTCGAGACGTGCTGCTGGCAGGGATGAAGGTGAATGGTGAAACAGCAGTGAAGTTGGGAATCACAGATGCAGCACACGACGGCGAAGAGGCACTGATGGAGGCGGCGGTGGCGATGGGGGAGAAGTTGGCAACGAGGAAGTGGGAAGGAGAAGCTTACGCGGAGATTAGGAAGAGTCTGTATCCTGAAATGTCTCCGTTGTTTAATGAACTGAGTGGAAAGCTGTAA

mRNA sequence

ATGTGCACACTTGAGAGGCGTGGCGATCTCTTCATCATCACCCTCACCGGCGACGACGAGCACTGGATCGGACCACCATTCATTTCCTCCTTCCTCTCTCTTCTGACCCAAATCAACTCCCAAGCCACGCCCGGTTCCGTTCTCATCACCACCTCCCATGGCCGCTTCTTCTCCAATGGCTTCCACCTTCCCTGGGTTCAATCCGCCGCTAGAGACCGCATACTCCACATGGTTCATATCTTCAAGCCTGTCGTCGCCGCCCTACTCTCTCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCATGCCGCCGCCGCCGGTTTTGCTCTCGCTCTTAGCCATGATTACGTCCTGATGAGGCGCGATAAAGGCGTGCTCTACATGAGCGAGATTGACTTGGGTTTTACGTTGCCCGATTATTTTGCGGCATTGATGAGGTCAAAGATCGGGTCAGCCTCAGTTCGTCGAGACGTGCTGCTGGCAGGGATGAAGGTGAATGGTGAAACAGCAGTGAAGTTGGGAATCACAGATGCAGCACACGACGGCGAAGAGGCACTGATGGAGGCGGCGGTGGCGATGGGGGAGAAGTTGGCAACGAGGAAGTGGGAAGGAGAAGCTTACGCGGAGATTAGGAAGAGTCTGTATCCTGAAATGTCTCCGTTGTTTAATGAACTGAGTGGAAAGCTGTAA

Coding sequence (CDS)

ATGTGCACACTTGAGAGGCGTGGCGATCTCTTCATCATCACCCTCACCGGCGACGACGAGCACTGGATCGGACCACCATTCATTTCCTCCTTCCTCTCTCTTCTGACCCAAATCAACTCCCAAGCCACGCCCGGTTCCGTTCTCATCACCACCTCCCATGGCCGCTTCTTCTCCAATGGCTTCCACCTTCCCTGGGTTCAATCCGCCGCTAGAGACCGCATACTCCACATGGTTCATATCTTCAAGCCTGTCGTCGCCGCCCTACTCTCTCTCCCCATGCCCACCATCGCCGCTGTCTCCGGCCATGCCGCCGCCGCCGGTTTTGCTCTCGCTCTTAGCCATGATTACGTCCTGATGAGGCGCGATAAAGGCGTGCTCTACATGAGCGAGATTGACTTGGGTTTTACGTTGCCCGATTATTTTGCGGCATTGATGAGGTCAAAGATCGGGTCAGCCTCAGTTCGTCGAGACGTGCTGCTGGCAGGGATGAAGGTGAATGGTGAAACAGCAGTGAAGTTGGGAATCACAGATGCAGCACACGACGGCGAAGAGGCACTGATGGAGGCGGCGGTGGCGATGGGGGAGAAGTTGGCAACGAGGAAGTGGGAAGGAGAAGCTTACGCGGAGATTAGGAAGAGTCTGTATCCTGAAATGTCTCCGTTGTTTAATGAACTGAGTGGAAAGCTGTAA

Protein sequence

MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNGFHLPWVQSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL
Homology
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BLAST of Carg06208 vs. NCBI nr
Match: KAG7028839.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 451.8 bits (1161), Expect = 3.5e-123
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMR 120
           FHLPWVQSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMR
Sbjct: 61  FHLPWVQSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMR 120

Query: 121 RDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAH 180
           RDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAH
Sbjct: 121 RDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAH 180

Query: 181 DGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           DGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL
Sbjct: 181 DGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 229

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BLAST of Carg06208 vs. NCBI nr
Match: KAG6597380.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 437.6 bits (1124), Expect = 6.7e-119
Identity = 225/234 (96.15%), Postives = 225/234 (96.15%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFA LMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFATLMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           TDAAHDGEEALMEAAVAMGEKLA RKWEGEAYAEIRKSLYP MSPLFNELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPGMSPLFNELSGKL 234

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BLAST of Carg06208 vs. NCBI nr
Match: XP_022975055.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima] >XP_022975341.1 enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 436.4 bits (1121), Expect = 1.5e-118
Identity = 224/234 (95.73%), Postives = 226/234 (96.58%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAARDRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           TDAAHDGEEALMEAAVAMGEKLA RKWEGEAYAEIRKSLYPEMSPL NELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]
BLAST of Carg06208 vs. NCBI nr
Match: XP_022942426.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 434.1 bits (1115), Expect = 7.5e-118
Identity = 223/234 (95.30%), Postives = 225/234 (96.15%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAARDRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAV+LGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVELGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           TDAAHDG EALMEAAVAMGEKLA RKWEGEAYAEIRKSLYPEMSPL NELSGKL
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]
BLAST of Carg06208 vs. NCBI nr
Match: XP_023540657.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 431.8 bits (1109), Expect = 3.7e-117
Identity = 221/234 (94.44%), Postives = 224/234 (95.73%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGDLFI+TLTGDDEHWIGPP ISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIVTLTGDDEHWIGPPLISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAA+DRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAAKDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRD+GVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDRGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           TDAAHDG EALMEAAVAMGEKLA RKWEGEAYAEIRKSLYPEMSPL NELSGKL
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]
BLAST of Carg06208 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 2.8e-75
Identity = 142/224 (63.39%), Postives = 180/224 (80.36%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RGDLF++TLTGD EH   P  I++ LSLL Q  SQ+T GS+LITT++G+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FHLPWVQSA-----ARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F L W Q+A     A +R+  MV  FKPVVAALL LPMPTIAA++GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YV MR+D+GVLYMSE+D+G ++PDYF+AL+R+KIG+++ RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 TD-AAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEM 219
            D AA+D EE ++ A+V +GEKLA +KW GE YA IRKSLYPE+
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPEL 224

[Close]
BLAST of Carg06208 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 4.0e-74
Identity = 142/222 (63.96%), Postives = 173/222 (77.93%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RGDLF++TLTG+DEH   P  I+S LSLL Q  SQ+T GSVLITT HG+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FHLPWVQSA---ARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYV 120
           F L W QSA   A  R+  MV  FKPV+AALL LPMPTIAA++GHAAA+G   ALSHDYV
Sbjct: 61  FDLAWAQSAGHGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHDYV 120

Query: 121 LMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITD 180
            MR+D+GVLYMSE+D+G  +PDYF+AL+ +K+GS   RR++LL+G K+ GE AV LGI D
Sbjct: 121 FMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGIVD 180

Query: 181 -AAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEM 219
            AAHD  E ++EA V++GE LA +KW GE YA IRKSLYPE+
Sbjct: 181 SAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPEL 222

[Close]
BLAST of Carg06208 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 5.3e-50
Identity = 107/219 (48.86%), Postives = 148/219 (67.58%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQAT-PGSVLITTSHGRFFSN 60
           MC+LE+R  LFI+ LTGD EH + P  + S  S + QI S  +   SVLITTS G+FFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFHLPWVQSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLM 120
           G+ L   +S     ++ M    + +VA L+SLPMPTIAAV+GHA+AAG  LA+SHDYVLM
Sbjct: 61  GYDLALAESNPSLSVV-MDAKLRSLVADLISLPMPTIAAVTGHASAAGCILAMSHDYVLM 120

Query: 121 RRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAA 180
           RRD+G LYMSE+D+   +P +F A++R KIGS + RRDV+L   KV  +  VK+GI D+A
Sbjct: 121 RRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMGIVDSA 180

Query: 181 HDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEM 219
           +      +EAA+ +GE++  R  +G  Y ++R+SL  E+
Sbjct: 181 YGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

[Close]
BLAST of Carg06208 vs. ExPASy Swiss-Prot
Match: B4SZ85 (Fatty acid oxidation complex subunit alpha OS=Salmonella newport (strain SL254) OX=423368 GN=fadB PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.0e-05
Identity = 41/150 (27.33%), Postives = 73/150 (48.67%), Query Frame = 0 

Query: 72  DRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMRRDKGVLYMSEI 131
           +++   +H    V   L  LP+PT+AAV+G+A   G    L+ DY L   D  +  + E 
Sbjct: 82  EQLSQWLHFANSVFNRLEDLPVPTLAAVNGYALGGGCECVLATDYRLATPDLRI-GLPET 141

Query: 132 DLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAHDGEEALMEAAV 191
            LG  +P +  ++   ++  A    +++ AG  V  E A+K+G+ D     +E L+E A+
Sbjct: 142 KLGI-MPGFGGSVRLPRMLGADSALEIIAAGKDVGAEHALKIGLVDGVVK-QETLIEGAI 201

Query: 192 AMGEKLATRKWEGEAYAEIRKSLYPEMSPL 222
           A+  +  T   +  A  +      P++ PL
Sbjct: 202 AVLRQAITGDLDWRAKRQ------PKLEPL 222

[Close]
BLAST of Carg06208 vs. ExPASy Swiss-Prot
Match: B7MHD1 (Fatty acid oxidation complex subunit alpha OS=Escherichia coli O45:K1 (strain S88 / ExPEC) OX=585035 GN=fadB PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 4.0e-05
Identity = 36/122 (29.51%), Postives = 63/122 (51.64%), Query Frame = 0 

Query: 72  DRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLMRRDKGVLYMSEI 131
           +++   +H    V   L  LP+PTIAAV+G+A   G    L+ DY L   D  +  + E 
Sbjct: 82  EQLSQWLHFANSVFNRLEDLPVPTIAAVNGYALGGGCECVLATDYRLATPDLRI-GLPET 141

Query: 132 DLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAAHDGEEALMEAAV 191
            LG  +P +  ++   ++  A    +++ AG  V  + A+K+G+ D     E+ L+E A+
Sbjct: 142 KLGI-MPGFGGSVRMPRMLGADSALEIIAAGKDVGADQALKIGLVDGVVKAEK-LVEGAI 200

Query: 192 AM 194
           A+
Sbjct: 202 AI 200

[Close]
BLAST of Carg06208 vs. ExPASy TrEMBL
Match: A0A6J1IDW4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111474523 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 7.3e-119
Identity = 224/234 (95.73%), Postives = 226/234 (96.58%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRG+LFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGNLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAARDRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           TDAAHDGEEALMEAAVAMGEKLA RKWEGEAYAEIRKSLYPEMSPL NELSGKL
Sbjct: 181 TDAAHDGEEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]
BLAST of Carg06208 vs. ExPASy TrEMBL
Match: A0A6J1FNU4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111447470 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.6e-118
Identity = 223/234 (95.30%), Postives = 225/234 (96.15%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG
Sbjct: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           FHLPW Q     SAARDRILHMVHIFKPV+AALLSLPMPTIAAVSGHAAAAGFALALSHD
Sbjct: 61  FHLPWAQSAASKSAARDRILHMVHIFKPVLAALLSLPMPTIAAVSGHAAAAGFALALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAV+LGI
Sbjct: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVELGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
           TDAAHDG EALMEAAVAMGEKLA RKWEGEAYAEIRKSLYPEMSPL NELSGKL
Sbjct: 181 TDAAHDGGEALMEAAVAMGEKLAKRKWEGEAYAEIRKSLYPEMSPLLNELSGKL 234

[Close]
BLAST of Carg06208 vs. ExPASy TrEMBL
Match: A0A6J1CRE1 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=LOC111013487 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.9e-104
Identity = 193/234 (82.48%), Postives = 213/234 (91.03%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RGD+FI+TLTGD+EHWIGPPF+SS LSLL+Q+ SQAT GSVL+TTSHGRFFSNG
Sbjct: 25  MCTLEKRGDVFILTLTGDNEHWIGPPFVSSLLSLLSQVKSQATRGSVLLTTSHGRFFSNG 84

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F LPW Q     SAA DR+ HMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGF LAL+HD
Sbjct: 85  FDLPWAQAAGSKSAATDRLAHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFLLALAHD 144

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           +VLMRRDKGVLYMSE+DLG T+PDYFAALMRSKIGSASVRRDVLL GMKVNGE+AVKLGI
Sbjct: 145 FVLMRRDKGVLYMSEVDLGLTMPDYFAALMRSKIGSASVRRDVLLTGMKVNGESAVKLGI 204

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
            DAAHDGEE LMEAAVAMG+KLA RKWEG AYA+IRKSLYPEM+PL + L+GK+
Sbjct: 205 ADAAHDGEEGLMEAAVAMGQKLAKRKWEGGAYAQIRKSLYPEMTPLLDGLNGKM 258

[Close]
BLAST of Carg06208 vs. ExPASy TrEMBL
Match: A0A1S3AXB9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483621 PE=4 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.1e-103
Identity = 194/234 (82.91%), Postives = 209/234 (89.32%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG LFI+TLTGD+EHWIGP FIS+F+SLL QI SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLEKRGSLFILTLTGDNEHWIGPTFISTFISLLAQIKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F LPW Q     SAARDRI+HMVHIFKP+VAALLSLPMPTIAA+SGHAAAAGFALAL HD
Sbjct: 61  FDLPWAQTAPSKSAARDRIVHMVHIFKPIVAALLSLPMPTIAAISGHAAAAGFALALCHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGV+YMSEIDLG T+PDYFAAL+RSKI S SVRRDVLLAG KVNGETA KLGI
Sbjct: 121 YVLMRRDKGVIYMSEIDLGLTMPDYFAALVRSKISSVSVRRDVLLAGRKVNGETAAKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
            D+ HD EEALMEAAVAMGE LA RKWE EAYAEIRKSLYPEMSPL NEL+GK+
Sbjct: 181 VDSVHDNEEALMEAAVAMGEMLARRKWESEAYAEIRKSLYPEMSPLLNELNGKM 234

[Close]
BLAST of Carg06208 vs. ExPASy TrEMBL
Match: A0A5A7U4L1 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001340 PE=4 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.5e-103
Identity = 194/234 (82.91%), Postives = 209/234 (89.32%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RG LFI+TLTGD+EHWIGP FIS+F+SLL QI SQAT GSVL+TTSHGRFFSNG
Sbjct: 1   MCTLEKRGSLFILTLTGDNEHWIGPTFISTFISLLAQIKSQATRGSVLLTTSHGRFFSNG 60

Query: 61  FHLPWVQ-----SAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F LPW Q     SAARDRI+HMVHIFKP+VAALLSLPMPTIAA+SGHAAAAGFALAL HD
Sbjct: 61  FDLPWAQTAPSKSAARDRIVHMVHIFKPIVAALLSLPMPTIAAISGHAAAAGFALALCHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YVLMRRDKGV+YMSEIDLG T+PDYFAAL+RSKI S SVRRDVLLAG KVNGETA KLGI
Sbjct: 121 YVLMRRDKGVIYMSEIDLGLTMPDYFAALVRSKISSVSVRRDVLLAGRKVNGETAAKLGI 180

Query: 181 TDAAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEMSPLFNELSGKL 230
            D+ HD EEALMEAAVAMGE LA RKWE EAYAEIRKSLYPEMSPL NEL+GK+
Sbjct: 181 IDSVHDNEEALMEAAVAMGEMLARRKWESEAYAEIRKSLYPEMSPLLNELNGKM 234

[Close]
BLAST of Carg06208 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 283.1 bits (723), Expect = 2.0e-76
Identity = 142/224 (63.39%), Postives = 180/224 (80.36%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RGDLF++TLTGD EH   P  I++ LSLL Q  SQ+T GS+LITT++G+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FHLPWVQSA-----ARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHD 120
           F L W Q+A     A +R+  MV  FKPVVAALL LPMPTIAA++GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YVLMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGI 180
           YV MR+D+GVLYMSE+D+G ++PDYF+AL+R+KIG+++ RR++LL+G K+ GE AV LGI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 TD-AAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEM 219
            D AA+D EE ++ A+V +GEKLA +KW GE YA IRKSLYPE+
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPEL 224

[Close]
BLAST of Carg06208 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 279.3 bits (713), Expect = 2.9e-75
Identity = 142/222 (63.96%), Postives = 173/222 (77.93%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQATPGSVLITTSHGRFFSNG 60
           MCTLE+RGDLF++TLTG+DEH   P  I+S LSLL Q  SQ+T GSVLITT HG+FFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FHLPWVQSA---ARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYV 120
           F L W QSA   A  R+  MV  FKPV+AALL LPMPTIAA++GHAAA+G   ALSHDYV
Sbjct: 61  FDLAWAQSAGHGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHDYV 120

Query: 121 LMRRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITD 180
            MR+D+GVLYMSE+D+G  +PDYF+AL+ +K+GS   RR++LL+G K+ GE AV LGI D
Sbjct: 121 FMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGIVD 180

Query: 181 -AAHDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEM 219
            AAHD  E ++EA V++GE LA +KW GE YA IRKSLYPE+
Sbjct: 181 SAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPEL 222

[Close]
BLAST of Carg06208 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 199.1 bits (505), Expect = 3.8e-51
Identity = 107/219 (48.86%), Postives = 148/219 (67.58%), Query Frame = 0 

Query: 1   MCTLERRGDLFIITLTGDDEHWIGPPFISSFLSLLTQINSQAT-PGSVLITTSHGRFFSN 60
           MC+LE+R  LFI+ LTGD EH + P  + S  S + QI S  +   SVLITTS G+FFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFHLPWVQSAARDRILHMVHIFKPVVAALLSLPMPTIAAVSGHAAAAGFALALSHDYVLM 120
           G+ L   +S     ++ M    + +VA L+SLPMPTIAAV+GHA+AAG  LA+SHDYVLM
Sbjct: 61  GYDLALAESNPSLSVV-MDAKLRSLVADLISLPMPTIAAVTGHASAAGCILAMSHDYVLM 120

Query: 121 RRDKGVLYMSEIDLGFTLPDYFAALMRSKIGSASVRRDVLLAGMKVNGETAVKLGITDAA 180
           RRD+G LYMSE+D+   +P +F A++R KIGS + RRDV+L   KV  +  VK+GI D+A
Sbjct: 121 RRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMGIVDSA 180

Query: 181 HDGEEALMEAAVAMGEKLATRKWEGEAYAEIRKSLYPEM 219
           +      +EAA+ +GE++  R  +G  Y ++R+SL  E+
Sbjct: 181 YGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREV 218

The following BLAST results are available for this feature:
BLAST of Carg06208 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7028839.13.5e-123100.00Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6597380.16.7e-11996.15Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022975055.11.5e-11895.73enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima] >XP_022975341.1... [more]
XP_022942426.17.5e-11895.30enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata][more]
XP_023540657.13.7e-11794.44enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
BLAST of Carg06208 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O232992.8e-7563.39Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2... [more]
O233004.0e-7463.96Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1[more]
O044695.3e-5048.86Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1... [more]
B4SZ853.0e-0527.33Fatty acid oxidation complex subunit alpha OS=Salmonella newport (strain SL254) ... [more]
B7MHD14.0e-0529.51Fatty acid oxidation complex subunit alpha OS=Escherichia coli O45:K1 (strain S8... [more]
BLAST of Carg06208 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1IDW47.3e-11995.73enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FNU43.6e-11895.30enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1CRE13.9e-10482.48enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=... [more]
A0A1S3AXB91.1e-10382.91enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7U4L11.5e-10382.91Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119... [more]
BLAST of Carg06208 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G14430.12.0e-7663.39indole-3-butyric acid response 10 [more]
AT4G14440.12.9e-7563.963-hydroxyacyl-CoA dehydratase 1 [more]
AT1G65520.13.8e-5148.86delta(3), delta(2)-enoyl CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 30..200
e-value: 5.7E-19
score: 68.4
NoneNo IPR availableGENE3D3.90.226.10coord: 1..224
e-value: 2.7E-36
score: 127.0
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 1..220
NoneNo IPR availablePANTHERPTHR11941:SF75ENOYL-COA DELTA ISOMERASE 2, PEROXISOMALcoord: 1..220
NoneNo IPR availableCDDcd06558crotonase-likecoord: 2..197
e-value: 1.82872E-17
score: 75.2896
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 3..201

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg06208-RACarg06208-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006635 fatty acid beta-oxidation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004165 dodecenoyl-CoA delta-isomerase activity
molecular_function GO:0004300 enoyl-CoA hydratase activity
molecular_function GO:0003824 catalytic activity