Carg06197 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06197
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Zeta_toxin domain-containing protein
Location	Carg_Chr06: 8562422 .. 8563693 (+)
RNA-Seq Expression	Carg06197
Synteny	Carg06197

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGCCGAGGACTAGCAATCAGAGATCCTCATCTTCTACCTCCGATCTCTCTGGTTTTTTTTTTTTTATAAAAAATTTCATCATGAAGCATGAAGGTAATCGAAGCTGTTTTCCTAACATTTCTTTGATTTTAATCTTTGAAATTCATTGAATATTAAAGACTAACTTTTCTGCTACATGCTCTGTGCTCAGATGGCGGCTTCGGGAAGGTCATTCCATCGTCGCACCATCTTAGCCCTTCGTTCGTCGGAGGCATCGCTGCTATCGGGAAAAGCCATTTCCGGCGGACTTCGAAGGCTGTAATCAACAATCACAAGTTGATTCCGCATCTACATATAATGGAGTCCGGCCGCGTTGGTTTCTTGGAGAGATTTTCGCATTATGTTGGTAAAGTCCTCCTTCGCTTTTACTGACTACAAACCTAATTCCATGTAAAATGGAATATCTAACCCTAATATTTTGAGGTTTTCATTTTGCAGCGAGGCAGTTAGGGCTTTCAGATGCAGATGAGTATCCTCAACTCTGCAAGCTAGCGAACAATTATTTGAGGAAGACGAATGGATGCGTGGAAGAAATTTATGCATATTTCGCGAGTGAATCCGAGGCGGAATCTCTGTATCTGAAGTTAATGGATGAGTTTGATCAATGCATCTCGAGTTATTTCGCATTCCATTGGAGCCAAGCTCCCTTGTTGATCAGTCAGGTAAATTCGTCAATCCTCCCCCAAAAAATGATGAACACGATGTTAGAATTTCAGTATATCCCTTAATTAATGGCGGATTTAATGATAGGTTTTGAGTGTCGACTCCGAGCGCAAGAAGCTGAAAGATCTTGTGGTGGCAGCGACTAGGTATTTTCATTTTAGATCTGTCAATTTGCTTGAGAAGTCAAATTTGGTTCCTGAGTTTGAACTTTGAAACTTCAATCATGGTGCATCTTCTAGAATAGAATTAAGATCCATTTGATAACTTCAATAAGTGAAGATTCTGAGTATTTATTTTCCTATATTGGTTTCTGGCCATTTGATAGGAAGGAGAGGTTCGATAGGGTGTGTAAGGATCTGAAAGTGACAAGGGTATTCTCTACTTTGGTGGAGGAAATGAAGAAGATAGGTTGTGCTCCATCTAAGGGCGAGTCGAAATTCTCCCACACGATACCGCCATCGTCTCGAAGAGATAGGAGGCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGCGCTGGCAAGAGTACTGTCCTCAAAGACATTTGCAAAGAGTGCGTAATATTGATATAG

mRNA sequence

AAGCCGAGGACTAGCAATCAGAGATCCTCATCTTCTACCTCCGATCTCTCTGGTTTTTTTTTTTTTATAAAAAATTTCATCATGAAGCATGAAGATGGCGGCTTCGGGAAGGTCATTCCATCGTCGCACCATCTTAGCCCTTCGTTCGTCGGAGGCATCGCTGCTATCGGGAAAAGCCATTTCCGGCGGACTTCGAAGGCTGTAATCAACAATCACAAGTTGATTCCGCATCTACATATAATGGAGTCCGGCCGCGTTGGTTTCTTGGAGAGATTTTCGCATTATGTTGCGAGGCAGTTAGGGCTTTCAGATGCAGATGAGTATCCTCAACTCTGCAAGCTAGCGAACAATTATTTGAGGAAGACGAATGGATGCGTGGAAGAAATTTATGCATATTTCGCGAGTGAATCCGAGGCGGAATCTCTGTATCTGAAGTTAATGGATGAGTTTGATCAATGCATCTCGAGTTATTTCGCATTCCATTGGAGCCAAGCTCCCTTGTTGATCAGTCAGGTTTTGAGTGTCGACTCCGAGCGCAAGAAGCTGAAAGATCTTGTGGTGGCAGCGACTAGGAAGGAGAGGTTCGATAGGGTGTGTAAGGATCTGAAAGTGACAAGGGTATTCTCTACTTTGGTGGAGGAAATGAAGAAGATAGGTTGTGCTCCATCTAAGGGCGAGTCGAAATTCTCCCACACGATACCGCCATCGTCTCGAAGAGATAGGAGGCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGCGCTGGCAAGAGTACTGTCCTCAAAGACATTTGCAAAGAGTGCGTAATATTGATATAG

Coding sequence (CDS)

ATGAAGCATGAAGATGGCGGCTTCGGGAAGGTCATTCCATCGTCGCACCATCTTAGCCCTTCGTTCGTCGGAGGCATCGCTGCTATCGGGAAAAGCCATTTCCGGCGGACTTCGAAGGCTGTAATCAACAATCACAAGTTGATTCCGCATCTACATATAATGGAGTCCGGCCGCGTTGGTTTCTTGGAGAGATTTTCGCATTATGTTGCGAGGCAGTTAGGGCTTTCAGATGCAGATGAGTATCCTCAACTCTGCAAGCTAGCGAACAATTATTTGAGGAAGACGAATGGATGCGTGGAAGAAATTTATGCATATTTCGCGAGTGAATCCGAGGCGGAATCTCTGTATCTGAAGTTAATGGATGAGTTTGATCAATGCATCTCGAGTTATTTCGCATTCCATTGGAGCCAAGCTCCCTTGTTGATCAGTCAGGTTTTGAGTGTCGACTCCGAGCGCAAGAAGCTGAAAGATCTTGTGGTGGCAGCGACTAGGAAGGAGAGGTTCGATAGGGTGTGTAAGGATCTGAAAGTGACAAGGGTATTCTCTACTTTGGTGGAGGAAATGAAGAAGATAGGTTGTGCTCCATCTAAGGGCGAGTCGAAATTCTCCCACACGATACCGCCATCGTCTCGAAGAGATAGGAGGCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGCGCTGGCAAGAGTACTGTCCTCAAAGACATTTGCAAAGAGTGCGTAATATTGATATAG

Protein sequence

MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVGFLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMDEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRVFSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKECVILI

Homology

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BLAST of Carg06197 vs. NCBI nr
Match: KAG7028850.1 (hypothetical protein SDJN02_10032 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 492.3 bits (1266), Expect = 2.5e-135
Identity = 244/244 (100.00%), Postives = 244/244 (100.00%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG
Sbjct: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM
Sbjct: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV
Sbjct: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKEC 240
           FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKEC
Sbjct: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKEC 240

Query: 241 VILI 245
           VILI
Sbjct: 241 VILI 244

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BLAST of Carg06197 vs. NCBI nr
Match: XP_022935075.1 (uncharacterized protein LOC111442050 [Cucurbita moschata])

HSP 1 Score: 478.0 bits (1229), Expect = 4.8e-131
Identity = 235/239 (98.33%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHI ESGRVG
Sbjct: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHITESGRVG 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLGLSDADEYPQLCKLANNYLR+TNGCVEEIYAYFASESEAESLYLKLM
Sbjct: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRETNGCVEEIYAYFASESEAESLYLKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCISSYFAFHWSQAPL++SQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV
Sbjct: 121 DEFDQCISSYFAFHWSQAPLMVSQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE
Sbjct: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 239

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BLAST of Carg06197 vs. NCBI nr
Match: XP_023540264.1 (uncharacterized protein LOC111800690 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 473.4 bits (1217), Expect = 1.2e-129
Identity = 234/239 (97.91%), Postives = 236/239 (98.74%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVIPSSHHLSPSFV GIAAIGKSHFRRTSKAVINNHKLIPHLHI ESGRVG
Sbjct: 1   MKHEDGGFGKVIPSSHHLSPSFVEGIAAIGKSHFRRTSKAVINNHKLIPHLHITESGRVG 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLGLSDAD+YPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM
Sbjct: 61  FLERFSHYVARQLGLSDADDYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCISSYFAFHWSQAPL+ISQVLSVDSER KLKDLVVAATRKERFDRVCKDLKVTRV
Sbjct: 121 DEFDQCISSYFAFHWSQAPLMISQVLSVDSERMKLKDLVVAATRKERFDRVCKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE
Sbjct: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 239

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BLAST of Carg06197 vs. NCBI nr
Match: KAG6597389.1 (Calmodulin calcium-dependent NAD kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 473.4 bits (1217), Expect = 1.2e-129
Identity = 234/235 (99.57%), Postives = 235/235 (100.00%), Query Frame = 0


Query: 5   DGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVGFLER 64
           DGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVGFLER
Sbjct: 450 DGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVGFLER 509

Query: 65  FSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMDEFD 124
           FSHYVARQLG+SDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMDEFD
Sbjct: 510 FSHYVARQLGISDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMDEFD 569

Query: 125 QCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRVFSTL 184
           QCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRVFSTL
Sbjct: 570 QCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRVFSTL 629

Query: 185 VEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           VEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE
Sbjct: 630 VEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 684

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BLAST of Carg06197 vs. NCBI nr
Match: XP_022975283.1 (uncharacterized protein LOC111474419 [Cucurbita maxima])

HSP 1 Score: 472.2 bits (1214), Expect = 2.6e-129
Identity = 234/239 (97.91%), Postives = 236/239 (98.74%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIP LHI ESGRVG
Sbjct: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPLLHITESGRVG 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKT GCVEEIYAYFASESEAESLYLKLM
Sbjct: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTKGCVEEIYAYFASESEAESLYLKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCISSYFAFHWSQAPL+ISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV
Sbjct: 121 DEFDQCISSYFAFHWSQAPLMISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCAPSKGESKFSHTIPPSSR+DRRPVLLLMGGGMGAGKSTVLKDICKE
Sbjct: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRKDRRPVLLLMGGGMGAGKSTVLKDICKE 239

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BLAST of Carg06197 vs. ExPASy Swiss-Prot
Match: Q0WUY1 (Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.2e-55
Identity = 116/196 (59.18%), Postives = 143/196 (72.96%), Query Frame = 0


Query: 46  KLIPHLHIMESGRVGFLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAY 105
           K+ P +   +SGR   LERFSHYVARQLG  D +EYPQLCKLAN YL KT G  E +  Y
Sbjct: 61  KIAPLIERKDSGRRSNLERFSHYVARQLGFEDPNEYPQLCKLANGYLLKTKGYDENVDEY 120

Query: 106 FASESEAESLYLKLMDEFDQCISSYFAFHWSQAPLLISQVLSVDSERK--KLKDLVVAAT 165
             +E+E +SLY+ L++EFD+CI +YF+F+W+Q+  LISQ LS +S++K  KLKD V+AAT
Sbjct: 121 LENEAERDSLYVHLLEEFDRCILTYFSFNWTQSSNLISQALSDESDQKVPKLKDFVMAAT 180

Query: 166 RKERFDRVCKDLKVTRVFSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGG 225
           RK+RF+RV KDLKV RV STLVEEM+ IG   S  E   +  + P +   R PVLLLMGG
Sbjct: 181 RKQRFERVTKDLKVKRVISTLVEEMRVIGSGSS--EPHCTEVMSPVAHNKRSPVLLLMGG 240

Query: 226 GMGAGKSTVLKDICKE 240
           GMGAGKSTVLKDI  E
Sbjct: 241 GMGAGKSTVLKDIFLE 254

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BLAST of Carg06197 vs. ExPASy TrEMBL
Match: A0A6J1F9J9 (uncharacterized protein LOC111442050 OS=Cucurbita moschata OX=3662 GN=LOC111442050 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.3e-131
Identity = 235/239 (98.33%), Postives = 238/239 (99.58%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHI ESGRVG
Sbjct: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHITESGRVG 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLGLSDADEYPQLCKLANNYLR+TNGCVEEIYAYFASESEAESLYLKLM
Sbjct: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRETNGCVEEIYAYFASESEAESLYLKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCISSYFAFHWSQAPL++SQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV
Sbjct: 121 DEFDQCISSYFAFHWSQAPLMVSQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE
Sbjct: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 239

[Close]

BLAST of Carg06197 vs. ExPASy TrEMBL
Match: A0A6J1IIR8 (uncharacterized protein LOC111474419 OS=Cucurbita maxima OX=3661 GN=LOC111474419 PE=4 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 1.3e-129
Identity = 234/239 (97.91%), Postives = 236/239 (98.74%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIP LHI ESGRVG
Sbjct: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPLLHITESGRVG 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKT GCVEEIYAYFASESEAESLYLKLM
Sbjct: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTKGCVEEIYAYFASESEAESLYLKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCISSYFAFHWSQAPL+ISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV
Sbjct: 121 DEFDQCISSYFAFHWSQAPLMISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCAPSKGESKFSHTIPPSSR+DRRPVLLLMGGGMGAGKSTVLKDICKE
Sbjct: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRKDRRPVLLLMGGGMGAGKSTVLKDICKE 239

[Close]

BLAST of Carg06197 vs. ExPASy TrEMBL
Match: A0A6J1J066 (uncharacterized protein LOC111480063 OS=Cucurbita maxima OX=3661 GN=LOC111480063 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.4e-107
Identity = 200/239 (83.68%), Postives = 211/239 (88.28%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVI SSH+L+ SFVGG+ A G  HFRRTSKAVINN KLIPHLH  ESGRV 
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLG+SDADE PQLCKLANNYLRKT GC EEIYAYFASE++ ESLY+KLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCI SYFAFHWSQA L+I+QVL VDSE KKLKDLVVAATRK+RFDRV KDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCA SKGESK SH  P  S+R R PVLLLMGGGMGAGKSTVLKDI KE
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKE 239

[Close]

BLAST of Carg06197 vs. ExPASy TrEMBL
Match: A0A6J1EBV0 (uncharacterized protein LOC111431771 OS=Cucurbita moschata OX=3662 GN=LOC111431771 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.4e-107
Identity = 200/239 (83.68%), Postives = 211/239 (88.28%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKHEDGGFGKVI SSH+L+ SFVGG+ A G  HFRRTSKAVINN KLIPHLH  ESGRV 
Sbjct: 1   MKHEDGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLIPHLHRTESGRVD 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLG+SDADE PQLCKLANNYLRKT GC EEIYAYFASE++ ESLY+KLM
Sbjct: 61  FLERFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLM 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           DEFDQCI SYFAFHWSQA L+I+QVL VDSE KKLKDLVVAATRK+RFDRV KDLKVTRV
Sbjct: 121 DEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCA SKGESK SH  P  S+R R PVLLLMGGGMGAGKSTVLKDI KE
Sbjct: 181 FSTLVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKE 239

[Close]

BLAST of Carg06197 vs. ExPASy TrEMBL
Match: A0A5A7U1T8 (P-loop nucleoside triphosphate hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001100 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.9e-105
Identity = 195/239 (81.59%), Postives = 211/239 (88.28%), Query Frame = 0


Query: 1   MKHEDGGFGKVIPSSHHLSPSFVGGIAAIGKSHFRRTSKAVINNHKLIPHLHIMESGRVG 60
           MKH+DGGFGKVIP+SH+L+ SFVGG++A G  HFRRTSK VINN KLIPHL   ESGRV 
Sbjct: 1   MKHQDGGFGKVIPTSHYLNTSFVGGVSATGNRHFRRTSKPVINNQKLIPHLRRTESGRVD 60

Query: 61  FLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLM 120
           FLERFSHYVARQLG+SD DE PQLCKLANNYLRKT GC EEIYAYFASE+E ESLY+KL+
Sbjct: 61  FLERFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCEEEIYAYFASEAEGESLYVKLV 120

Query: 121 DEFDQCISSYFAFHWSQAPLLISQVLSVDSERKKLKDLVVAATRKERFDRVCKDLKVTRV 180
           +EFDQCI SYFAFHWSQA L+I+QVL VDSE KKLKDLVVAATRK+RFDRV KDLK+TRV
Sbjct: 121 NEFDQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKMTRV 180

Query: 181 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           FSTLVEEMKKIGCA SKGESK S T P SS+R R PVLLLMGGGMGAGKSTVLKDI KE
Sbjct: 181 FSTLVEEMKKIGCAASKGESKCSLTTPRSSQRQRSPVLLLMGGGMGAGKSTVLKDILKE 239

[Close]

BLAST of Carg06197 vs. TAIR 10
Match: AT2G30630.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 224.6 bits (571), Expect = 8.9e-59
Identity = 111/179 (62.01%), Postives = 143/179 (79.89%), Query Frame = 0


Query: 62  LERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMD 121
           LERFSHYV RQ+G  D  E P LCKLAN Y+RK+  C E+IY++F+ E  A+SL++KL++
Sbjct: 72  LERFSHYVVRQMGFKDRRECPHLCKLANEYIRKSGSCEEDIYSFFSEEPGADSLFIKLVE 131

Query: 122 EFDQCISSYFAFHWSQAPLLISQVLSVDSE-RKKLKDLVVAATRKERFDRVCKDLKVTRV 181
           EF++CI SYFA+HWS A L+ISQ+LS D E +KKLK +V+AATR++RF+RV K+LKV RV
Sbjct: 132 EFERCILSYFAYHWSHADLMISQILSADVEPKKKLKHIVMAATREQRFERVTKNLKVARV 191

Query: 182 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           F+TLVEEMK +G A S  +S+ +  + P + +DR PVLLLMGGGMGAGKSTVLKDI KE
Sbjct: 192 FNTLVEEMKAMGIA-SVDDSECTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKE 249

[Close]

BLAST of Carg06197 vs. TAIR 10
Match: AT1G04280.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 8.3e-57
Identity = 116/196 (59.18%), Postives = 143/196 (72.96%), Query Frame = 0


Query: 46  KLIPHLHIMESGRVGFLERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAY 105
           K+ P +   +SGR   LERFSHYVARQLG  D +EYPQLCKLAN YL KT G  E +  Y
Sbjct: 61  KIAPLIERKDSGRRSNLERFSHYVARQLGFEDPNEYPQLCKLANGYLLKTKGYDENVDEY 120

Query: 106 FASESEAESLYLKLMDEFDQCISSYFAFHWSQAPLLISQVLSVDSERK--KLKDLVVAAT 165
             +E+E +SLY+ L++EFD+CI +YF+F+W+Q+  LISQ LS +S++K  KLKD V+AAT
Sbjct: 121 LENEAERDSLYVHLLEEFDRCILTYFSFNWTQSSNLISQALSDESDQKVPKLKDFVMAAT 180

Query: 166 RKERFDRVCKDLKVTRVFSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGG 225
           RK+RF+RV KDLKV RV STLVEEM+ IG   S  E   +  + P +   R PVLLLMGG
Sbjct: 181 RKQRFERVTKDLKVKRVISTLVEEMRVIGSGSS--EPHCTEVMSPVAHNKRSPVLLLMGG 240

Query: 226 GMGAGKSTVLKDICKE 240
           GMGAGKSTVLKDI  E
Sbjct: 241 GMGAGKSTVLKDIFLE 254

[Close]

BLAST of Carg06197 vs. TAIR 10
Match: AT1G06750.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 107/179 (59.78%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 62  LERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMD 121
           LERFSHYVARQ+G  D  E P LCKLA  Y+ K+  C E+IY++F+ E +A++L++KL++
Sbjct: 58  LERFSHYVARQMGFKDRRECPNLCKLAAEYISKSECCEEDIYSFFSEEPDADTLFIKLVE 117

Query: 122 EFDQCISSYFAFHWSQAPLLISQVLSVDSE-RKKLKDLVVAATRKERFDRVCKDLKVTRV 181
           EF++CI SYFAFHWS A L+I+QVLS D+E ++KLK +V+AATR+ R  RV K+LKV RV
Sbjct: 118 EFERCILSYFAFHWSHADLMITQVLSADAEPKRKLKQIVMAATRELRIKRVTKNLKVARV 177

Query: 182 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           F+TLVEEM+ +G A S  +S+ +  + P + +DR PVLLLMGGGMGAGKSTVLKDI KE
Sbjct: 178 FNTLVEEMRAMGLA-SVDDSQCTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKE 235

[Close]

BLAST of Carg06197 vs. TAIR 10
Match: AT1G06750.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 107/179 (59.78%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 62  LERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMD 121
           LERFSHYVARQ+G  D  E P LCKLA  Y+ K+  C E+IY++F+ E +A++L++KL++
Sbjct: 65  LERFSHYVARQMGFKDRRECPNLCKLAAEYISKSECCEEDIYSFFSEEPDADTLFIKLVE 124

Query: 122 EFDQCISSYFAFHWSQAPLLISQVLSVDSE-RKKLKDLVVAATRKERFDRVCKDLKVTRV 181
           EF++CI SYFAFHWS A L+I+QVLS D+E ++KLK +V+AATR+ R  RV K+LKV RV
Sbjct: 125 EFERCILSYFAFHWSHADLMITQVLSADAEPKRKLKQIVMAATRELRIKRVTKNLKVARV 184

Query: 182 FSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLLLMGGGMGAGKSTVLKDICKE 240
           F+TLVEEM+ +G A S  +S+ +  + P + +DR PVLLLMGGGMGAGKSTVLKDI KE
Sbjct: 185 FNTLVEEMRAMGLA-SVDDSQCTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKE 242

[Close]

BLAST of Carg06197 vs. TAIR 10
Match: AT2G30630.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 3.5e-55
Identity = 112/200 (56.00%), Postives = 143/200 (71.50%), Query Frame = 0


Query: 62  LERFSHYVARQLGLSDADEYPQLCKLANNYLRKTNGCVEEIYAYFASESEAESLYLKLMD 121
           LERFSHYV RQ+G  D  E P LCKLAN Y+RK+  C E+IY++F+ E  A+SL++KL++
Sbjct: 72  LERFSHYVVRQMGFKDRRECPHLCKLANEYIRKSGSCEEDIYSFFSEEPGADSLFIKLVE 131

Query: 122 EFDQCISSYFAFHWSQAPLLISQV---------------------LSVDSE-RKKLKDLV 181
           EF++CI SYFA+HWS A L+ISQV                     LS D E +KKLK +V
Sbjct: 132 EFERCILSYFAYHWSHADLMISQVTNKLSLSLLCYITNRPKTFQILSADVEPKKKLKHIV 191

Query: 182 VAATRKERFDRVCKDLKVTRVFSTLVEEMKKIGCAPSKGESKFSHTIPPSSRRDRRPVLL 240
           +AATR++RF+RV K+LKV RVF+TLVEEMK +G A S  +S+ +  + P + +DR PVLL
Sbjct: 192 MAATREQRFERVTKNLKVARVFNTLVEEMKAMGIA-SVDDSECTEVMAPVAHKDRSPVLL 251

The following BLAST results are available for this feature:

BLAST of Carg06197 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028850.1	2.5e-135	100.00	hypothetical protein SDJN02_10032 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022935075.1	4.8e-131	98.33	uncharacterized protein LOC111442050 [Cucurbita moschata]	[more]
XP_023540264.1	1.2e-129	97.91	uncharacterized protein LOC111800690 [Cucurbita pepo subsp. pepo]	[more]
KAG6597389.1	1.2e-129	99.57	Calmodulin calcium-dependent NAD kinase, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_022975283.1	2.6e-129	97.91	uncharacterized protein LOC111474419 [Cucurbita maxima]	[more]

BLAST of Carg06197 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WUY1	1.2e-55	59.18	Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC...	[more]

BLAST of Carg06197 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F9J9	2.3e-131	98.33	uncharacterized protein LOC111442050 OS=Cucurbita moschata OX=3662 GN=LOC1114420...	[more]
A0A6J1IIR8	1.3e-129	97.91	uncharacterized protein LOC111474419 OS=Cucurbita maxima OX=3661 GN=LOC111474419...	[more]
A0A6J1J066	1.4e-107	83.68	uncharacterized protein LOC111480063 OS=Cucurbita maxima OX=3661 GN=LOC111480063...	[more]
A0A6J1EBV0	1.4e-107	83.68	uncharacterized protein LOC111431771 OS=Cucurbita moschata OX=3662 GN=LOC1114317...	[more]
A0A5A7U1T8	4.9e-105	81.59	P-loop nucleoside triphosphate hydrolase superfamily protein OS=Cucumis melo var...	[more]

BLAST of Carg06197 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30630.1	8.9e-59	62.01	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT1G04280.1	8.3e-57	59.18	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT1G06750.1	1.2e-55	59.78	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT1G06750.2	1.2e-55	59.78	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT2G30630.2	3.5e-55	56.00	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 200..243 e-value: 2.9E-5 score: 25.3
IPR010488	Zeta toxin domain	PFAM	PF06414	Zeta_toxin	coord: 211..239 e-value: 2.5E-5 score: 23.7
IPR044802	Calmodulin calcium-dependent NAD kinase NADKc-like	PANTHER	PTHR31153	UNCHARACTERIZED	coord: 25..241
None	No IPR available	PANTHER	PTHR31153:SF18	PUTATIVE, EXPRESSED-RELATED	coord: 25..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06197-RA	Carg06197-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0016020	membrane
cellular_component	GO:0015935	small ribosomal subunit
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016301	kinase activity