Carg05709 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg05709 vs. NCBI nr
Match: KAG6589014.1 (hypothetical protein SDJN03_17579, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015277.1 ureH [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 732.6 bits (1890), Expect = 1.7e-207
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
           LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. NCBI nr
Match: XP_022928346.1 (uncharacterized protein LOC111435202 [Cucurbita moschata])

HSP 1 Score: 726.9 bits (1875), Expect = 9.3e-206
Identity = 378/382 (98.95%), Postives = 381/382 (99.74%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
           LIHCLSSSNS SDSISTS+SRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           CSRMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIG+LGI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. NCBI nr
Match: XP_023529731.1 (uncharacterized protein LOC111792465 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.4 bits (1866), Expect = 1.0e-204
Identity = 377/382 (98.69%), Postives = 379/382 (99.21%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSK +KPSMP
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKTEKPSMP 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
           LIHCLSSSNSSSDSISTSDSRA SPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRAGSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           CSRMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPCVVGLENGECDVGIYKTLE PMVIGEKNKKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. NCBI nr
Match: XP_022989236.1 (uncharacterized protein LOC111486376 [Cucurbita maxima])

HSP 1 Score: 721.5 bits (1861), Expect = 3.9e-204
Identity = 376/382 (98.43%), Postives = 378/382 (98.95%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           MDRLLYSPSPTSFKINPNPPLLLPRFRRIDS KLNFPLPTRHGLRPLGL SSKV+KPSMP
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPSMP 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
           LIHCLSSSNSSSDSISTSDSRA SPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           CSRMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPCVVGLENGECDVGIYKTLE PMVIGEKNKKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. NCBI nr
Match: XP_022135872.1 (uncharacterized protein LOC111007714 [Momordica charantia])

HSP 1 Score: 617.1 bits (1590), Expect = 1.0e-172
Identity = 328/382 (85.86%), Postives = 348/382 (91.10%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           M+RLLYSPSPTS KINP+  L LPRFR I SAKLNFP+PTRHGLR +GL S K +KP + 
Sbjct: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
            I  LS  NSSSDSI  SDSRA SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           C+RMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGV+GI
Sbjct: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPC V LENGECDVGI++TL+ PMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPC-VALENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 376

BLAST of Carg05709 vs. ExPASy Swiss-Prot
Match: Q07404 (Urease accessory protein UreH OS=Bacillus sp. (strain TB-90) OX=36824 GN=ureH PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.0e-09
Identity = 61/228 (26.75%), Postives = 94/228 (41.23%), Query Frame = 0

Query: 146 QSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCSRM-ESAAVGALWGCGHDAGQLIF 205
           Q +LLS    GF  G  H +  PDH+ A++ +     ++  S+  G  WG GH +  LIF
Sbjct: 3   QVDLLSILTLGFVLGIKHAME-PDHVIAVSTIVCQSKKLWRSSLAGVFWGIGHTSTLLIF 62

Query: 206 GLLFLLLKDKLHIEILRTWG---TIVVGVTLFVIGVLGIREASEVRTPCVVGLENGECDV 265
           G+  +L+K K+  E    W      +VG+ L   G+  I    +        L +   D 
Sbjct: 63  GMTIILMKKKISQE----WSMSLEFLVGIILVYFGISAILSLKKTHEHSHSRL-HLHTDH 122

Query: 266 GIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLV 325
            IY     P V              +   G++HGL   A M++L    +     G  +++
Sbjct: 123 PIYTYKGIPYV-------------KSLFIGIIHGLAGSAAMVLLTMSTVEKAWEGLLYIL 182

Query: 326 MFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAASFVAIALGL 370
            F  GTV+ M S+T  IG     L  R  RI         F++   G+
Sbjct: 183 FFGAGTVLGMLSFTTLIG-IPFTLSARKIRIHNAFIQITGFISTVFGI 210

BLAST of Carg05709 vs. ExPASy TrEMBL
Match: A0A6J1EK24 (uncharacterized protein LOC111435202 OS=Cucurbita moschata OX=3662 GN=LOC111435202 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 4.5e-206
Identity = 378/382 (98.95%), Postives = 381/382 (99.74%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
           LIHCLSSSNS SDSISTS+SRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSLSDSISTSNSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           CSRMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIG+LGI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGILGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. ExPASy TrEMBL
Match: A0A6J1JJH7 (uncharacterized protein LOC111486376 OS=Cucurbita maxima OX=3661 GN=LOC111486376 PE=4 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 1.9e-204
Identity = 376/382 (98.43%), Postives = 378/382 (98.95%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           MDRLLYSPSPTSFKINPNPPLLLPRFRRIDS KLNFPLPTRHGLRPLGL SSKV+KPSMP
Sbjct: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSVKLNFPLPTRHGLRPLGLLSSKVEKPSMP 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
           LIHCLSSSNSSSDSISTSDSRA SPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP
Sbjct: 61  LIHCLSSSNSSSDSISTSDSRASSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           CSRMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI
Sbjct: 181 CSRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPCVVGLENGECDVGIYKTLE PMVIGEKNKKKKKIGFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPCVVGLENGECDVGIYKTLEKPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA
Sbjct: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. ExPASy TrEMBL
Match: A0A6J1C3Z9 (uncharacterized protein LOC111007714 OS=Momordica charantia OX=3673 GN=LOC111007714 PE=4 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 5.0e-173
Identity = 328/382 (85.86%), Postives = 348/382 (91.10%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           M+RLLYSPSPTS KINP+  L LPRFR I SAKLNFP+PTRHGLR +GL S K +KP + 
Sbjct: 1   MERLLYSPSPTSLKINPSRSLFLPRFRLIGSAKLNFPIPTRHGLRSIGLISCKNEKPPLS 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
            I  LS  NSSSDSI  SDSRA SPK DLLEHF GK+KVK+SLFVLSALALILIQPVFAP
Sbjct: 61  SIRSLSLFNSSSDSICPSDSRAASPKLDLLEHFGGKEKVKRSLFVLSALALILIQPVFAP 120

Query: 121 AAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180
           AA ASF NAAKTG PAAAAVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIG
Sbjct: 121 AASASFPNAAKTGAPAAAAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIG 180

Query: 181 CSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGI 240
           C+RMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGT+VVGVTLF+IGV+GI
Sbjct: 181 CNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTVVVGVTLFIIGVMGI 240

Query: 241 REASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDA 300
           REASEVRTPC V LENGECDVGI++TL+ PMV      KKKK+GFATFATGVVHGLQPDA
Sbjct: 241 REASEVRTPC-VALENGECDVGIFETLDKPMV-----GKKKKLGFATFATGVVHGLQPDA 300

Query: 301 LMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAA 360
           LMIVLPALALPSR+AGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITE+LTWAA
Sbjct: 301 LMIVLPALALPSRIAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITERLTWAA 360

Query: 361 SFVAIALGLAIIISQFLGYSLY 383
           SFVAIALGLAIIISQFLGYSLY
Sbjct: 361 SFVAIALGLAIIISQFLGYSLY 376

BLAST of Carg05709 vs. ExPASy TrEMBL
Match: A0A1S3BJI9 (uncharacterized protein LOC103490284 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490284 PE=4 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.3e-170
Identity = 323/380 (85.00%), Postives = 341/380 (89.74%), Query Frame = 0

Query: 4   LLYSPSPTSFKINPNPPLLLPRFRR-IDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMPLI 63
           LLYS SPTS  INPN  L+LPRF R I S + NFPLPTRHG  PLG  S K DKPS   I
Sbjct: 5   LLYSSSPTSLNINPNSSLVLPRFARIIHSLRPNFPLPTRHGFHPLGFLSFKNDKPSFSSI 64

Query: 64  HCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAPAA 123
            C SS NSS+DSI  SDS   SPKPD L+ FA K+KVK SLFVLSALALILIQPVFAPAA
Sbjct: 65  PCFSSPNSSADSIFQSDSPPDSPKPDFLKPFARKEKVKSSLFVLSALALILIQPVFAPAA 124

Query: 124 FASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCS 183
           FASFQNAAKTGGPAA AVGRRLIQSELL+SAWTGFFAGCLHTLSGPDHLAALAPLSIGCS
Sbjct: 125 FASFQNAAKTGGPAAVAVGRRLIQSELLNSAWTGFFAGCLHTLSGPDHLAALAPLSIGCS 184

Query: 184 RMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGIRE 243
           RMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTI+VGVTLFVIG++GIRE
Sbjct: 185 RMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIIVGVTLFVIGIMGIRE 244

Query: 244 ASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPDALM 303
           ASE+RTP VV L+NGECDVGIY+TLE PMV+G  NKK KK+GFATFATGVVHGLQPDALM
Sbjct: 245 ASEIRTPFVVALDNGECDVGIYETLEKPMVVG--NKKSKKLGFATFATGVVHGLQPDALM 304

Query: 304 IVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWAASF 363
           IVLPALALPSRVAGAAFL+MFLVGTVISMGSYTAFIG+FSEALKDRVPRITE+LTW ASF
Sbjct: 305 IVLPALALPSRVAGAAFLLMFLVGTVISMGSYTAFIGTFSEALKDRVPRITERLTWVASF 364

Query: 364 VAIALGLAIIISQFLGYSLY 383
           VAIALGLAIIISQFLGYSLY
Sbjct: 365 VAIALGLAIIISQFLGYSLY 382

BLAST of Carg05709 vs. ExPASy TrEMBL
Match: A0A6J1I158 (uncharacterized protein LOC111468477 OS=Cucurbita maxima OX=3661 GN=LOC111468477 PE=4 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 9.8e-169
Identity = 326/383 (85.12%), Postives = 344/383 (89.82%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPNPPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPSMP 60
           M+RLLYSPSPTS KIN N  L L    RIDS KLNFPLPT HGL+PLGL   K +KPSM 
Sbjct: 1   MERLLYSPSPTSLKINLNSSLTLLWSGRIDSLKLNFPLPTLHGLKPLGLTFCKNEKPSMS 60

Query: 61  LIHCLSSSNSSSDSISTSDSRAVSPKPDLLEHFAGKKKVKQSLFVLSALALILIQPVFAP 120
              CLSSSN  SDSI  SDS A SPKPD LEHFAGK+KVK+SL VLSAL LILIQP  AP
Sbjct: 61  SFCCLSSSNWLSDSIFPSDSLAGSPKPDFLEHFAGKEKVKRSLCVLSALVLILIQPGLAP 120

Query: 121 AAFASFQN-AAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180
           AAFASFQN AAKTGGPAAA VGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI
Sbjct: 121 AAFASFQNAAAKTGGPAAAVVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHLAALAPLSI 180

Query: 181 GCSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVTLFVIGVLG 240
           GC+RMESAAVGALWGCGHDAGQ+IFGLLFLLLKDKLHIEILRTWGTIVVG+TLF+IGV+G
Sbjct: 181 GCNRMESAAVGALWGCGHDAGQVIFGLLFLLLKDKLHIEILRTWGTIVVGMTLFIIGVMG 240

Query: 241 IREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATGVVHGLQPD 300
           IRE SEVRTPC V LENGECDVGIY+TLE PMV+G+  KKKKKIGFATFATGVVHGLQPD
Sbjct: 241 IREVSEVRTPC-VALENGECDVGIYETLEKPMVVGK--KKKKKIGFATFATGVVHGLQPD 300

Query: 301 ALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPRITEKLTWA 360
           ALMIVLPALALPSRVAGAAFL+MFLVGTV+SMGSYTAFIGSFSEALKDRVPRITE+LTWA
Sbjct: 301 ALMIVLPALALPSRVAGAAFLLMFLVGTVLSMGSYTAFIGSFSEALKDRVPRITERLTWA 360

Query: 361 ASFVAIALGLAIIISQFLGYSLY 383
           ASFVAIALGLAIII+ FLG+SLY
Sbjct: 361 ASFVAIALGLAIIITHFLGFSLY 380

BLAST of Carg05709 vs. TAIR 10
Match: AT2G16800.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 408.7 bits (1049), Expect = 5.2e-114
Identity = 236/393 (60.05%), Postives = 286/393 (72.77%), Query Frame = 0

Query: 1   MDRLLYSPS-----PTSFKINPNPPLLLPRFRRIDSAKLN-FPLPTRHGLRPLGLFSSKV 60
           MDRLL  PS     P+ F+  P+P LL     R+ S  L+ FP   R   R +   S   
Sbjct: 1   MDRLLQPPSSHSIAPSKFQSRPSPLLL----HRLHSTNLSTFPSSRRLESRRISSISCFF 60

Query: 61  DKPSMPLIHCLSSSNSSSDSISTSDSRAVSPKPDLLEH----FAGKKKVKQSLFVL-SAL 120
            +   P       + SS+  I++S + A  P P  ++     F  +K +   + +L SA+
Sbjct: 61  RQNPSP-DTSPGLNQSSNFLIASSQTDASKPNPGFIQRIVSSFEQRKTISAGMVILVSAI 120

Query: 121 ALILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPD 180
           A +L+ P+  P AFASFQ A  +GG  AA VG +L+++E+L+SAWTGFFAGCLHTLSGPD
Sbjct: 121 AALLLNPILVPPAFASFQTATNSGG--AAVVGGKLLRTEVLTSAWTGFFAGCLHTLSGPD 180

Query: 181 HLAALAPLSIGCSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVG 240
           HLAALAPLSIG +RMESAAVGALWGCGHDAGQLIFGLLFLLLKD+LHIE++RTWGT VVG
Sbjct: 181 HLAALAPLSIGRTRMESAAVGALWGCGHDAGQLIFGLLFLLLKDRLHIEVIRTWGTRVVG 240

Query: 241 VTLFVIGVLGIREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFA 300
           +TL VIG +GI+EASE+  PCVV LENGE D              EK+ KKKKIGFATFA
Sbjct: 241 LTLLVIGAMGIKEASEMPEPCVVTLENGETD--------------EKSLKKKKIGFATFA 300

Query: 301 TGVVHGLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRV 360
           TG+VHGLQPDALM+VLPALALPSR+AGA+FL+MFL+GTVI+MGSYT FIGS SEALK++V
Sbjct: 301 TGIVHGLQPDALMMVLPALALPSRIAGASFLIMFLIGTVIAMGSYTVFIGSCSEALKEKV 360

Query: 361 PRITEKLTWAASFVAIALGLAIIISQFLGYSLY 383
           PRITEKLTWA+S VAI LGLAII+SQF G+SLY
Sbjct: 361 PRITEKLTWASSLVAIGLGLAIIVSQFFGFSLY 372

BLAST of Carg05709 vs. TAIR 10
Match: AT4G35080.1 (high-affinity nickel-transport family protein )

HSP 1 Score: 369.8 bits (948), Expect = 2.7e-102
Identity = 219/391 (56.01%), Postives = 274/391 (70.08%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPN--PPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPS 60
           M+RLL  PS +S  I+P+  P    P   R+ S+ L+F    R   R +   S   +   
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVS--SISCNSSQ 60

Query: 61  MPLIHCLSSSNSSSDSISTS-DSRAVSPKPDLLEHFAGKKKVKQ------SLFVLSALAL 120
           +P ++    SN++++S + S  S    P P  L   A     ++      ++ ++SA+A+
Sbjct: 61  IPSLYTPIGSNTTNNSFNGSPKSDESKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 121 ILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHL 180
           +L+ P+ AP AFASFQ AAK+G                L+SAWTGF AGCLHTLSGPDHL
Sbjct: 121 LLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDHL 180

Query: 181 AALAPLSIGCSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVT 240
           AALAPLSIG S+MESAAVGALWGCGHDAGQ+IFGLLFLLLKD+LHIE+L+TWGT +VG+T
Sbjct: 181 AALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGLT 240

Query: 241 LFVIGVLGIREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATG 300
           L +IG +GI+EASE+  PCV      E D+ +  T +  + +     KKKKIGFATFATG
Sbjct: 241 LVIIGAMGIKEASEIPEPCVA----LETDISMVSTEKEALPL----PKKKKIGFATFATG 300

Query: 301 VVHGLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPR 360
           VVHGLQPDALMIVLPALALPSR+AG+AFL+MFLVGTVI+MGSYTAFIGS SEALK++VPR
Sbjct: 301 VVHGLQPDALMIVLPALALPSRLAGSAFLIMFLVGTVIAMGSYTAFIGSCSEALKEKVPR 360

Query: 361 ITEKLTWAASFVAIALGLAIIISQFLGYSLY 383
           ITEKLTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 ITEKLTWVSSLVAIGLGLGIVISPFFGFSLY 365

BLAST of Carg05709 vs. TAIR 10
Match: AT4G35080.3 (high-affinity nickel-transport family protein )

HSP 1 Score: 362.1 bits (928), Expect = 5.6e-100
Identity = 221/415 (53.25%), Postives = 277/415 (66.75%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPN--PPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPS 60
           M+RLL  PS +S  I+P+  P    P   R+ S+ L+F    R   R +   S   +   
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVS--SISCNSSQ 60

Query: 61  MPLIHCLSSSNSSSDSISTS-DSRAVSPKPDLLEHFAG------------------KKKV 120
           +P ++    SN++++S + S  S    P P  L   A                   +KK+
Sbjct: 61  IPSLYTPIGSNTTNNSFNGSPKSDESKPNPGFLTRIATSASEQRKETYEKFVYSLLEKKI 120

Query: 121 KQ------------SLFVLSALALILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQS 180
            +            ++ ++SA+A++L+ P+ AP AFASFQ AAK+G              
Sbjct: 121 PRITKSSNFTLSTGTVILISAVAVLLLNPLLAPPAFASFQTAAKSG-------------- 180

Query: 181 ELLSSAWTGFFAGCLHTLSGPDHLAALAPLSIGCSRMESAAVGALWGCGHDAGQLIFGLL 240
             L+SAWTGF AGCLHTLSGPDHLAALAPLSIG S+MESAAVGALWGCGHDAGQ+IFGLL
Sbjct: 181 -WLTSAWTGFLAGCLHTLSGPDHLAALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLL 240

Query: 241 FLLLKDKLHIEILRTWGTIVVGVTLFVIGVLGIREASEVRTPCVVGLENGECDVGIYKTL 300
           FLLLKD+LHIE+L+TWGT +VG+TL +IG +GI+EASE+  PCV      E D+ +  T 
Sbjct: 241 FLLLKDRLHIEVLQTWGTRIVGLTLVIIGAMGIKEASEIPEPCVA----LETDISMVSTE 300

Query: 301 ENPMVIGEKNKKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRVAGAAFLVMFLVGT 360
           +  + +     KKKKIGFATFATGVVHGLQPDALMIVLPALALPSR+AG+AFL+MFLVGT
Sbjct: 301 KEALPL----PKKKKIGFATFATGVVHGLQPDALMIVLPALALPSRLAGSAFLIMFLVGT 360

Query: 361 VISMGSYTAFIGSFSEALKDRVPRITEKLTWAASFVAIALGLAIIISQFLGYSLY 383
           VI+MGSYTAFIGS SEALK++VPRITEKLTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 VIAMGSYTAFIGSCSEALKEKVPRITEKLTWVSSLVAIGLGLGIVISPFFGFSLY 389

BLAST of Carg05709 vs. TAIR 10
Match: AT4G35080.2 (high-affinity nickel-transport family protein )

HSP 1 Score: 311.2 bits (796), Expect = 1.1e-84
Identity = 195/391 (49.87%), Postives = 246/391 (62.92%), Query Frame = 0

Query: 1   MDRLLYSPSPTSFKINPN--PPLLLPRFRRIDSAKLNFPLPTRHGLRPLGLFSSKVDKPS 60
           M+RLL  PS +S  I+P+  P    P   R+ S+ L+F    R   R +   S   +   
Sbjct: 1   MERLL-QPSSSSSSISPSKFPSRTSPFLPRLRSSGLSFVSTHRPESRRVS--SISCNSSQ 60

Query: 61  MPLIHCLSSSNSSSDSISTS-DSRAVSPKPDLLEHFAGKKKVKQ------SLFVLSALAL 120
           +P ++    SN++++S + S  S    P P  L   A     ++      ++ ++SA+A+
Sbjct: 61  IPSLYTPIGSNTTNNSFNGSPKSDESKPNPGFLTRIATSASEQRKTLSTGTVILISAVAV 120

Query: 121 ILIQPVFAPAAFASFQNAAKTGGPAAAAVGRRLIQSELLSSAWTGFFAGCLHTLSGPDHL 180
           +L+ P+ AP AFASFQ AAK+G                L+SAWTGF AGCLHTLSGPDHL
Sbjct: 121 LLLNPLLAPPAFASFQTAAKSG---------------WLTSAWTGFLAGCLHTLSGPDHL 180

Query: 181 AALAPLSIGCSRMESAAVGALWGCGHDAGQLIFGLLFLLLKDKLHIEILRTWGTIVVGVT 240
           AALAPLSIG S+MESAAVGALWGCGHDAGQ+IFGLLFLLLKD+LHIE+L+TWGT +VG+T
Sbjct: 181 AALAPLSIGRSKMESAAVGALWGCGHDAGQVIFGLLFLLLKDRLHIEVLQTWGTRIVGLT 240

Query: 241 LFVIGVLGIREASEVRTPCVVGLENGECDVGIYKTLENPMVIGEKNKKKKKIGFATFATG 300
           L +IG +GI+EASE+  PCV      E D+ +  T +  + +     KKKKIGFATFATG
Sbjct: 241 LVIIGAMGIKEASEIPEPCVA----LETDISMVSTEKEALPL----PKKKKIGFATFATG 300

Query: 301 VVHGLQPDALMIVLPALALPSRVAGAAFLVMFLVGTVISMGSYTAFIGSFSEALKDRVPR 360
           VVHGLQPDALMIVLPALALPSR                             EALK++VPR
Sbjct: 301 VVHGLQPDALMIVLPALALPSR-----------------------------EALKEKVPR 336

Query: 361 ITEKLTWAASFVAIALGLAIIISQFLGYSLY 383
           ITEKLTW +S VAI LGL I+IS F G+SLY
Sbjct: 361 ITEKLTWVSSLVAIGLGLGIVISPFFGFSLY 336