Carg05330 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05330
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationCarg_Chr07: 4274253 .. 4277948 (+)
RNA-Seq ExpressionCarg05330
SyntenyCarg05330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCCCTTCCTTACATTGTCGAAGATTGCATGGGCGTGCTCCAACTCTACAGCGACGGCACCGTCTTCCGCTCCGACGAAATCCGTTTCCCGTTTCCAATCACCGACGACAGCTCCGTCGTCTTCCGCGACGTTCTCTACGACGCCGTTCACGGTCTCCATCTCCGTCTCATAAAACCAGCAGTATCATTAAATTCCAACACTAAACTTCCAGTCCTGTTCTTCTTCCACGGCGGCGGTTTCTGCGTCGGTTCTCGATCCTGGGCGAATCCGCACAATTGCTGCGTTCGGCTTGCCCTAGGGTTGGAAGCACTAGTAATCGTACCGGACTACCGTCTAGCGCCGGAAAATCGGCTTCCTGCGGCGGTGGAAGACGGAGAAAAAGCGATCGAGTGGGTGAGAAAAGGGGAAGACGCGTGGATTGAAGAAGGAGCGGATTTGGAGAGGGTATTTGTGATGGGAGACTCGTCGGGAGGGAACATTGCGCATCATTTGGCGGTAGGGTTTGGGAACGATCGTGGAAGATTTGGAGTGAGAATTAGAGGATTTGTTTTAATGGCGCCGTTCTTCGGCGGAGTGGTGAGGACGAAATCAGAGGAGGGTCCGCCGGAAGATTTTCTGAACTTGGACGCGCTTGACAGGTACACGTCCTCCTCTCTCTCTAACTATAAGATTCCCTTTTATTTATTTATTTATTTATTTATTTATAATATTAATAATTCTGCATTTTTTTTCTTCAATAAATCTTAATTTAGTAACTTTTTTAAAAAATAAATTAATATTTCTCTGTAAATTTTCAAAAATATGATTTGTTTTTTATACAACATTTAGGGCTAATTACTTAATTTTACTCTTTTTAGTAAAAGAAAAAAAAATAAATAACTATAAAAATTAATATTAAGATTTATAAAATAGACAGAGAAAATTGATATTTTAAATTTAAAATTATCACAACATTATATTATTTAAAATTGGTAGTGATGTCATTTTTAAATTATTATTTATTTATTATTATTATTATTAGAAGTTATATTGTTTAAATATTTTATCTAAAATCAATTGCACAATGCAAATGACGAAAATAACCCTAGATAAATATCACAAAATTTCAGAAGTATTTTTTTAAAAAACACATAAATACCACAAAATTTCATTTTTAAAATCAGAATATTTTAATCAATTTCTTACCCAATTTTGGCTTTTAAAAATTCAGTCTACCCATGCAGTATATATATATATATATATATATATATTTCTATCCATTGGTCTAAACATTTGATTTTTTTTTTTTTTTAATCTCTCAATTTTTATGATTCTTTGAAGAAATTATTTATAAGATAATAAATTAGTTATTTTGTTTTTGGAAAAAAAGAAAAAGGGGGAGTGAGAAAATAATAAAAAGTAGAAGAAAAAGAAAGAAAAGAAGGCATGCAGTGCAAATGGATGATGGCAAAATAGTCCAAAACCAAATATTTGACTTTCTTTTCTTCTCAATCTAACCTAATCTTCTATCATCTTCCCAAACATCTATTTCCACCTCATCACCCACTCCACGTGGCTATTATTCTTTTCCTAGTCAGCCACAAAAATATTATTAATTTTCTTTTTTTAAAAAAAATAAAAAAAACGTATATTTCTATTTTGAATGTTAAAGGTTGAAATCTATGGTCCACTTCTAACCACTCTCTTATTATTTATTTATTTTATTAAAAAATTATGAATTTAAGAAAATAATTTCTTAGAAATGTCATATTCAAATCTCCACCAGGTAAAAGGAGAGATCTAGTCCATGCCTTTGACTTTTGACCCACCATTATCAAACCACAACTTCCTGGATTTATTGCTAAAATCCAAATCACACAATATATATACCTAAACTAAAATAATTATAATTATAATAATATAAAAATACCAAAATTTAGAGTTCATTAAAAATAAATTTGGTGTAATTTGAGTTCAATATTTTAGATTAAGCTTAGGTTTGAAATTAGTATCTCAAGATGGTGAGATAAGATTCAATTAAATTTGTTCTTCGTCAGCAAATAAAAAATAAAAATATAATGTTTGGAGCCACATTTTTTTCCTTCATTTATGATATTTTGTAAGAGCACTTATAAATTATAATGCATTTAGAAAGTGACTTTAGGGGAGAAAAACATTATTAAAGTTTTAATTATAATATTTTTTTGAAATCTGTTAGGATTTAGCCACTTTATTTATATTTATTTAAATAAAAAATTTAATGGATAAGTTCATTCAATTAAACTTATCCATTAATTTAATTTATAATATGATTGTTTTTTTGTTTTGTTTTTGTTTTGTTTCATAGTGGAGTTACAATCAACTGATTATAGTTTAAACAAAATTATGGAGGAGACAATGATTAAACAATGAAATAAAAGAAGCATAGTGGAAGGTTTAATAATCTCATACCCTAATTATCCACTAACTAAATTACATTTTCTTAATATTCTTTCCTTAAATCTCACACATAATTCATAAATAAAGTTCGAATCTTTTGGAAAAAGAGAGAGATAGCGACTTATGGATTCTAACCAGATCTCTATGCTTCATGATCTCTATGCTTCATGGATTCTAACCAGATGTCACAACCCAACCACTCAAACCTATACTTATTTGTGGATAACACTATAGAGTATATACAATAGATATATATATAATTTCTCAAACAAGGTTTTAGATTTTTTTATTTTTAATATCTGAATTTTGAGTAATTCAAGCCTTTTAAAAAATAAAAAATAAAAGTTGGTAACAGTCCAAATCTACTACTAGTATATTTGTCTTTTTTGGCTCTCTCTTTTGGGCTTCTACTCCTAAGTTTTAAAATGTGTCGGTTAGGAAGAAGTTTCTATACCATTATAAAGACTCTATTTTGGGCTTGCTAAGAAAAAGTTTTCACACCCTTATAAAGACTTTCTCTTTTGGGCTTCTACTCGAAGTTTTAAAACGTGTCTGTTAGGAAGAAATTTTCACACCCTTATAAAGACTCTCTTTTGAGCTTCGAGGTTTTAAAACGTGTCTGTTAGGAAGAAGTTTCCACACCCTTATAAAGACTCTTTTGGTCTTCTACTCAAAGTTTTAAAACGGGTCTGTTAAGAGAAAGTTTCCACACCCTTATAAAGAATGGTTCGTAAGGTCTTACAATCTACCCTCCTTTAGGGCCAACGTCCTTGCTGGCACTCGTTCTCCTCTCTAATCGATGTGGGATCTCTCAATAGGTTACTATCATTGAAATTATTTATAAACATCAAAAAGATGTCCTCCTTCAAGTATCGATGTCAATTCGAGTAGATGGATGACATTACTAAGATTCGAACACATGTCTTTTGTGAACACTTTAATGAGTCTCACAATTGATGGTTCAGGTTTTGGAGGTTATCATTACCCTTGGGAGAAGATAGAGACCACCCATTGGCAAACCCATTTGGGCCATCAAGCAAGAGCCTTGAAGGAGTGAATTTGGAGCCGATCATGGTGATGGTTGGTGAAAATGAATTGCTGAAAGACAGAGTCCAACACTATGCCAAAACACTCTCACAATTGGGACACAGAATTGAGTATGTGGAGTTTGAAGGCAAACAACATGGGTTCTTCACTAGTTCTCAAGATACTCAACTGGGCGATCAAGTCCTGGCTATTATCAAACGTTTTATGCTTCAAAATTCTCTTTGA

mRNA sequence

ATGGGTTCCCTTCCTTACATTGTCGAAGATTGCATGGGCGTGCTCCAACTCTACAGCGACGGCACCGTCTTCCGCTCCGACGAAATCCGTTTCCCGTTTCCAATCACCGACGACAGCTCCGTCGTCTTCCGCGACGTTCTCTACGACGCCGTTCACGGTCTCCATCTCCGTCTCATAAAACCAGCAGTATCATTAAATTCCAACACTAAACTTCCAGTCCTGTTCTTCTTCCACGGCGGCGGTTTCTGCGTCGGTTCTCGATCCTGGGCGAATCCGCACAATTGCTGCGTTCGGCTTGCCCTAGGGTTGGAAGCACTAGTAATCGTACCGGACTACCGTCTAGCGCCGGAAAATCGGCTTCCTGCGGCGGTGGAAGACGGAGAAAAAGCGATCGAGTGGGTGAGAAAAGGGGAAGACGCGTGGATTGAAGAAGGAGCGGATTTGGAGAGGGTATTTGTGATGGGAGACTCGTCGGGAGGGAACATTGCGCATCATTTGGCGGTAGGGTTTGGGAACGATCGTGGAAGATTTGGAGTGAGAATTAGAGGATTTGTTTTAATGGCGCCGTTCTTCGGCGGAGTGGTGAGGACGAAATCAGAGGAGGGTCCGCCGGAAGATTTTCTGAACTTGGACGCGCTTGACAGGTTTTGGAGGTTATCATTACCCTTGGGAGAAGATAGAGACCACCCATTGGCAAACCCATTTGGGCCATCAAGCAAGAGCCTTGAAGGAGTGAATTTGGAGCCGATCATGGTGATGGTTGGTGAAAATGAATTGCTGAAAGACAGAGTCCAACACTATGCCAAAACACTCTCACAATTGGGACACAGAATTGAGTATGTGGAGTTTGAAGGCAAACAACATGGGTTCTTCACTAGTTCTCAAGATACTCAACTGGGCGATCAAGTCCTGGCTATTATCAAACGTTTTATGCTTCAAAATTCTCTTTGA

Coding sequence (CDS)

ATGGGTTCCCTTCCTTACATTGTCGAAGATTGCATGGGCGTGCTCCAACTCTACAGCGACGGCACCGTCTTCCGCTCCGACGAAATCCGTTTCCCGTTTCCAATCACCGACGACAGCTCCGTCGTCTTCCGCGACGTTCTCTACGACGCCGTTCACGGTCTCCATCTCCGTCTCATAAAACCAGCAGTATCATTAAATTCCAACACTAAACTTCCAGTCCTGTTCTTCTTCCACGGCGGCGGTTTCTGCGTCGGTTCTCGATCCTGGGCGAATCCGCACAATTGCTGCGTTCGGCTTGCCCTAGGGTTGGAAGCACTAGTAATCGTACCGGACTACCGTCTAGCGCCGGAAAATCGGCTTCCTGCGGCGGTGGAAGACGGAGAAAAAGCGATCGAGTGGGTGAGAAAAGGGGAAGACGCGTGGATTGAAGAAGGAGCGGATTTGGAGAGGGTATTTGTGATGGGAGACTCGTCGGGAGGGAACATTGCGCATCATTTGGCGGTAGGGTTTGGGAACGATCGTGGAAGATTTGGAGTGAGAATTAGAGGATTTGTTTTAATGGCGCCGTTCTTCGGCGGAGTGGTGAGGACGAAATCAGAGGAGGGTCCGCCGGAAGATTTTCTGAACTTGGACGCGCTTGACAGGTTTTGGAGGTTATCATTACCCTTGGGAGAAGATAGAGACCACCCATTGGCAAACCCATTTGGGCCATCAAGCAAGAGCCTTGAAGGAGTGAATTTGGAGCCGATCATGGTGATGGTTGGTGAAAATGAATTGCTGAAAGACAGAGTCCAACACTATGCCAAAACACTCTCACAATTGGGACACAGAATTGAGTATGTGGAGTTTGAAGGCAAACAACATGGGTTCTTCACTAGTTCTCAAGATACTCAACTGGGCGATCAAGTCCTGGCTATTATCAAACGTTTTATGCTTCAAAATTCTCTTTGA

Protein sequence

MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIKPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVRIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLGDQVLAIIKRFMLQNSL
Homology
BLAST of Carg05330 vs. NCBI nr
Match: KAG7027165.1 (putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 653.7 bits (1685), Expect = 8.3e-184
Identity = 316/316 (100.00%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK
Sbjct: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60

Query: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120
           PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL
Sbjct: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120

Query: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180
           PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR
Sbjct: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180

Query: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240
           IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK
Sbjct: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240

Query: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300
           SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG
Sbjct: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300

Query: 301 DQVLAIIKRFMLQNSL 317
           DQVLAIIKRFMLQNSL
Sbjct: 301 DQVLAIIKRFMLQNSL 316

BLAST of Carg05330 vs. NCBI nr
Match: XP_022963184.1 (probable carboxylesterase 15 [Cucurbita moschata])

HSP 1 Score: 652.1 bits (1681), Expect = 2.4e-183
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK
Sbjct: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60

Query: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120
           PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL
Sbjct: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120

Query: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180
           PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR
Sbjct: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180

Query: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240
           IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK
Sbjct: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240

Query: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300
           SLEGVNLEPIMVMVGENELLK+RVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG
Sbjct: 241 SLEGVNLEPIMVMVGENELLKERVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300

Query: 301 DQVLAIIKRFMLQNSL 317
           DQVLAIIKRFMLQNSL
Sbjct: 301 DQVLAIIKRFMLQNSL 316

BLAST of Carg05330 vs. NCBI nr
Match: XP_023517355.1 (probable carboxylesterase 15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.4 bits (1679), Expect = 4.1e-183
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK
Sbjct: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60

Query: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120
           PAVSLNSNTKLPVLFF HGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL
Sbjct: 61  PAVSLNSNTKLPVLFFLHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120

Query: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180
           PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR
Sbjct: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180

Query: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240
           IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK
Sbjct: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240

Query: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300
           SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG
Sbjct: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300

Query: 301 DQVLAIIKRFMLQNSL 317
           DQVLAIIKRFMLQNSL
Sbjct: 301 DQVLAIIKRFMLQNSL 316

BLAST of Carg05330 vs. NCBI nr
Match: XP_023003802.1 (probable carboxylesterase 15 [Cucurbita maxima])

HSP 1 Score: 637.5 bits (1643), Expect = 6.1e-179
Identity = 306/316 (96.84%), Postives = 312/316 (98.73%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLPYIVEDCMGVLQLYSDGTVFRSD+IRFPFPI DDSSVVFRDVLYDAVHGLHLRLIK
Sbjct: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDDIRFPFPIADDSSVVFRDVLYDAVHGLHLRLIK 60

Query: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120
           PAVSLNSNTKLPVLFF HGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL
Sbjct: 61  PAVSLNSNTKLPVLFFLHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120

Query: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180
           PAAVEDGEKAIEWV+KGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFG+D GRFGVR
Sbjct: 121 PAAVEDGEKAIEWVKKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGSDGGRFGVR 180

Query: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240
           +RGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGP SK
Sbjct: 181 VRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPLSK 240

Query: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300
           +LEG+NLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG
Sbjct: 241 NLEGMNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300

Query: 301 DQVLAIIKRFMLQNSL 317
           DQVLAIIKRFMLQNSL
Sbjct: 301 DQVLAIIKRFMLQNSL 316

BLAST of Carg05330 vs. NCBI nr
Match: KAG6595149.1 (putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 629.8 bits (1623), Expect = 1.3e-176
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0

Query: 12  MGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIKPAVSLNSNTKL 71
           MGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIKPAVSLNSNTKL
Sbjct: 1   MGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIKPAVSLNSNTKL 60

Query: 72  PVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVEDGEKAI 131
           PVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVEDGEKAI
Sbjct: 61  PVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVEDGEKAI 120

Query: 132 EWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVRIRGFVLMAPFF 191
           EWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVRIRGFVLMAPFF
Sbjct: 121 EWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVRIRGFVLMAPFF 180

Query: 192 GGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSKSLEGVNLEPIM 251
           GGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSKSLEGVNLEPIM
Sbjct: 181 GGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSKSLEGVNLEPIM 240

Query: 252 VMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLGDQVLAIIKRFM 311
           VMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLGDQVLAIIKRFM
Sbjct: 241 VMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLGDQVLAIIKRFM 300

Query: 312 LQNSL 317
           LQNSL
Sbjct: 301 LQNSL 305

BLAST of Carg05330 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.8e-94
Identity = 173/323 (53.56%), Postives = 227/323 (70.28%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRF---PFPITDDSSVVFRDVLYDAVHGLHLR 60
           +G  P + EDCMG+LQL S+GTV RS+ I       P  ++ +V+F+D +Y   + LHLR
Sbjct: 4   LGEEPQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLR 63

Query: 61  LIKPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPE 120
           L KP +S ++ T LPV+ FFHGGGFC GSRSW + HN C+ LA  L ALV+ PDYRLAPE
Sbjct: 64  LYKP-ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPE 123

Query: 121 NRLPAAVEDGEKAIEW-----VRKGEDAWIEEGADL--ERVFVMGDSSGGNIAHHLAVGF 180
           +RLPAA ED E  + W     V  G + W E+G D+  +RVFV+GDSSGGNIAH LAV F
Sbjct: 124 HRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRF 183

Query: 181 GNDRGRF-GVRIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDH 240
           G+       VR+RG+VLM PFFGG  RT SE GP E  L+LD LD+FWRLSLP G  RDH
Sbjct: 184 GSGSIELTPVRVRGYVLMGPFFGGEERTNSENGPSEALLSLDLLDKFWRLSLPNGATRDH 243

Query: 241 PLANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQL-GHRIEYVEFEGKQH 300
            +ANPFGP+S +LE ++LEP++V+VG +ELL+DR + YA  L ++ G R++Y+EFE K+H
Sbjct: 244 HMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEH 303

Query: 301 GFFTSSQDTQLGDQVLAIIKRFM 312
           GF+++   ++  +QVL II  FM
Sbjct: 304 GFYSNYPSSEAAEQVLRIIGDFM 325

BLAST of Carg05330 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.5e-42
Identity = 102/308 (33.12%), Postives = 159/308 (51.62%), Query Frame = 0

Query: 7   IVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIKPAVSLN 66
           +VE+  G++++++DG V R   +    P    SS      +  +        I  A + +
Sbjct: 28  VVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLSNDTWTRVYIPDAAAAS 87

Query: 67  SNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVED 126
            +  LP+L +FHGGGFCVGS +W+  H+    LA+    +++  +YRLAPE+RLPAA +D
Sbjct: 88  PSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYDD 147

Query: 127 GEKAIEWVRK-------GEDAWIEEGADLERVFVMGDSSGGNIAHHLAVG-FGNDRGRFG 186
           G   + W+ K       G  +W+ +  +L  VF+ GDS+G NIA+ +AV    + +    
Sbjct: 148 GVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVRIMASGKYANT 207

Query: 187 VRIRGFVLMAPFFGGVVRTKSEE---GPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPF 246
           + ++G +L+ PFFGG  RT SE+         L L A D +WRL+LP G  RDHP  NP 
Sbjct: 208 LHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCNPL 267

Query: 247 GPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQ 304
             S+    G  L   MV + E ++LK+R     K +   G R+E +   G  H F     
Sbjct: 268 MSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHILDN 327

BLAST of Carg05330 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.1e-41
Identity = 102/267 (38.20%), Postives = 146/267 (54.68%), Query Frame = 0

Query: 7   IVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDS-----SVVFRDVLYDAVHGLHLRLIKP 66
           +V++  G++++Y DG V RS  +    P  D S      V   DV+ D +  +  RL  P
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLL----PCVDPSLPLELGVTCSDVVIDKLTNVWARLYVP 82

Query: 67  AVSLNSN-TKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 126
             +  S+ +KLP++ +FHGGGFCVGS SW   H    RL+     LV+  +YRLAPEN L
Sbjct: 83  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 142

Query: 127 PAAVEDGEKAIEWVRK--GEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFG 186
           PAA EDG  AI W+ K   ++ W ++  D  R+F+ GDS+GGNIA  +A    +      
Sbjct: 143 PAAYEDGVNAILWLNKARNDNLWAKQ-CDFGRIFLAGDSAGGNIAQQVAARLASPED-LA 202

Query: 187 VRIRGFVLMAPFFGGVVRTKSEEGPPED---FLNLDALDRFWRLSLPLGEDRDHPLANPF 246
           ++I G +L+ PF+ G  RT+SE     D    L L + D +WR+SLP G +R+HP   P 
Sbjct: 203 LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPV 262

Query: 247 GPSSKSLEGVNLEPIMVMVGENELLKD 263
               KS     +   +V V E +LL D
Sbjct: 263 KMIIKS---STVTRTLVCVAEMDLLMD 280

BLAST of Carg05330 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 9.0e-40
Identity = 97/248 (39.11%), Postives = 139/248 (56.05%), Query Frame = 0

Query: 46  VLYDAVHGLHLRLIKPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEA 105
           +L+   HG  L L KP   L++   +PVL FFHGG F   S + A     C RL      
Sbjct: 85  LLHQTRHGT-LELTKP---LSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGV 144

Query: 106 LVIVPDYRLAPENRLPAAVEDGEKAIEWVRKGEDAWIEEGADLE-RVFVMGDSSGGNIAH 165
           +V+  DYR +PE+R P A +DG  A+ WV+     W++ G D    V++ GDSSGGNIAH
Sbjct: 145 VVVSVDYRRSPEHRYPCAYDDGWNALNWVK--SRVWLQSGKDSNVYVYLAGDSSGGNIAH 204

Query: 166 HLAVGFGNDRGRFGVRIRGFVLMAPFFGGVVRTKSEEG-PPEDFLNLDALDRFWRLSLPL 225
           ++AV   N+    GV++ G +L+ P FGG  RT+SE+    + F+ +   D +WR  LP 
Sbjct: 205 NVAVRATNE----GVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPE 264

Query: 226 GEDRDHPLANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEF 285
           GEDRDHP  NPFGP  +SL+GVN    +V+V   +L++D    Y   L + G  +  +  
Sbjct: 265 GEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYL 322

Query: 286 EGKQHGFF 292
           +    GF+
Sbjct: 325 KQATIGFY 322

BLAST of Carg05330 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 107/306 (34.97%), Postives = 167/306 (54.58%), Query Frame = 0

Query: 20  DGTVFRSDEIRFP-FPITDDSS----VVFRDVLYDAVHGLHLRLIKPAVSLN----SNTK 79
           DG++ R D   FP    T D S     V +D+  + +    LRL  P+ ++N    S+ K
Sbjct: 21  DGSITR-DLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQK 80

Query: 80  LPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVEDGEKA 139
           LP++ ++HGGGF + S      H+ C  +A  L A+V+ P YRLAPE+RLPAA +DG +A
Sbjct: 81  LPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEA 140

Query: 140 IEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFG-VRIRGFVLMAP 199
           ++W++  +D WI+  AD   VF+MG S+GGN+A+++ +   +       ++IRG +L  P
Sbjct: 141 LDWIKTSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHHP 200

Query: 200 FFGGVVRTKSEEGPPEDFLNLDAL-DRFWRLSLPLGEDRDHPLANP-FGPSSKSLEGVN- 259
           FFGG  R++SE     D +    + D  W LSLP+G DRDH  +NP  G  S+ LE +  
Sbjct: 201 FFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIGR 260

Query: 260 -LEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLGDQVLA 312
               +M++ GE++ + D  +  AK + + G  +E VE     H      +D      +  
Sbjct: 261 LRWKVMMIGGEDDPMIDLQKDVAKLMKKKG--VEVVEHYTGGHVHGAEIRDPSKRKTLFL 320

BLAST of Carg05330 vs. ExPASy TrEMBL
Match: A0A6J1HJC4 (probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111463468 PE=4 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.2e-183
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK
Sbjct: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60

Query: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120
           PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL
Sbjct: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120

Query: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180
           PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR
Sbjct: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180

Query: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240
           IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK
Sbjct: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240

Query: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300
           SLEGVNLEPIMVMVGENELLK+RVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG
Sbjct: 241 SLEGVNLEPIMVMVGENELLKERVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300

Query: 301 DQVLAIIKRFMLQNSL 317
           DQVLAIIKRFMLQNSL
Sbjct: 301 DQVLAIIKRFMLQNSL 316

BLAST of Carg05330 vs. ExPASy TrEMBL
Match: A0A6J1KNM6 (probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111497276 PE=4 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.0e-179
Identity = 306/316 (96.84%), Postives = 312/316 (98.73%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLPYIVEDCMGVLQLYSDGTVFRSD+IRFPFPI DDSSVVFRDVLYDAVHGLHLRLIK
Sbjct: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDDIRFPFPIADDSSVVFRDVLYDAVHGLHLRLIK 60

Query: 61  PAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120
           PAVSLNSNTKLPVLFF HGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL
Sbjct: 61  PAVSLNSNTKLPVLFFLHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 120

Query: 121 PAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGVR 180
           PAAVEDGEKAIEWV+KGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFG+D GRFGVR
Sbjct: 121 PAAVEDGEKAIEWVKKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGSDGGRFGVR 180

Query: 181 IRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSSK 240
           +RGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGP SK
Sbjct: 181 VRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPLSK 240

Query: 241 SLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300
           +LEG+NLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG
Sbjct: 241 NLEGMNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQLG 300

Query: 301 DQVLAIIKRFMLQNSL 317
           DQVLAIIKRFMLQNSL
Sbjct: 301 DQVLAIIKRFMLQNSL 316

BLAST of Carg05330 vs. ExPASy TrEMBL
Match: A0A6J1BWM5 (probable carboxylesterase 15 OS=Momordica charantia OX=3673 GN=LOC111005401 PE=4 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.6e-145
Identity = 253/317 (79.81%), Postives = 279/317 (88.01%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITD-DSSVVFRDVLYDAVHGLHLRLI 60
           MGSLP+IVEDCMGVLQLYSDGTV RSD+I FPFPI   DSSVVFRDVLYDA HGLHLRL 
Sbjct: 1   MGSLPHIVEDCMGVLQLYSDGTVSRSDDINFPFPIAAYDSSVVFRDVLYDAAHGLHLRLF 60

Query: 61  KPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENR 120
           KPA S  S  KLPVL+F HGGGFCVGSRSW N HNCC RLALGLEALV+ PDYRLAPENR
Sbjct: 61  KPATS--SRAKLPVLYFIHGGGFCVGSRSWPNSHNCCFRLALGLEALVVAPDYRLAPENR 120

Query: 121 LPAAVEDGEKAIEWVRKGEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFGV 180
           LPAAVEDG +AIEW+R+  D WI++  DLERVFVMGDSSGGNIAHHLAVGF     R  V
Sbjct: 121 LPAAVEDGGRAIEWLRREADEWIDDRVDLERVFVMGDSSGGNIAHHLAVGF-----RSSV 180

Query: 181 RIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPFGPSS 240
           R+RGF+LMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLP+GEDRDHPLANPFGP S
Sbjct: 181 RVRGFILMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPVGEDRDHPLANPFGPRS 240

Query: 241 KSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQDTQL 300
             LEGV+L PIMVMVG NE+LKDRVQ+YAKTL+QLG++I+YVEFEGKQHGFFT+ QD+Q 
Sbjct: 241 MGLEGVDLGPIMVMVGGNEMLKDRVQNYAKTLAQLGNKIQYVEFEGKQHGFFTNCQDSQF 300

Query: 301 GDQVLAIIKRFMLQNSL 317
           GD+++AIIK F++QNSL
Sbjct: 301 GDEIVAIIKHFIIQNSL 310

BLAST of Carg05330 vs. ExPASy TrEMBL
Match: A0A5D3C8G7 (Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001890 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.8e-139
Identity = 252/325 (77.54%), Postives = 274/325 (84.31%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLP+IVEDCMGVLQLYSDGTV RSD I FPFPIT D+SVVFRDVLYDA H LHLRL K
Sbjct: 1   MGSLPHIVEDCMGVLQLYSDGTVSRSDNIHFPFPITLDTSVVFRDVLYDASHALHLRLYK 60

Query: 61  PAVSLNS-------NTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYR 120
           P  S +S       N KLP+LFFFHGGGFCVGSRSW N HNCCVRLALGL+ALVI PDYR
Sbjct: 61  PPSSSSSSSSSPTTNKKLPILFFFHGGGFCVGSRSWPNSHNCCVRLALGLDALVIAPDYR 120

Query: 121 LAPENRLPAAVEDGEKAIEWVRKGE--DAWIEEGADLERVFVMGDSSGGNIAHHLAVGFG 180
           LAPE+RLPAAVEDG KAIEWVRK    D WIEE  DL+RVFVMGDSSGGNIAHHLAV  G
Sbjct: 121 LAPEHRLPAAVEDGVKAIEWVRKAGKLDEWIEESGDLKRVFVMGDSSGGNIAHHLAVRIG 180

Query: 181 NDRGRFGVRIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPL 240
            +  +FGV  RGFVLMAPFFGGV RTKSEEGP E   +L+ALDRFWRLSLP+GEDRDHPL
Sbjct: 181 IENEKFGV--RGFVLMAPFFGGVRRTKSEEGPAEQLFDLEALDRFWRLSLPIGEDRDHPL 240

Query: 241 ANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFF 300
           ANPFG SS SLE V LEPI+V+VG +E+LKDR ++YAKTLSQLG RIEYVEFEGKQHGFF
Sbjct: 241 ANPFGASSMSLEEVKLEPILVIVGGDEMLKDRAENYAKTLSQLGKRIEYVEFEGKQHGFF 300

Query: 301 TSSQDTQLGDQVLAIIKRFMLQNSL 317
           T+SQD QLG QV+AIIK+FML NS+
Sbjct: 301 TNSQDPQLGHQVIAIIKKFMLHNSV 323

BLAST of Carg05330 vs. ExPASy TrEMBL
Match: A0A1S3CHD3 (probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103500946 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.8e-139
Identity = 252/325 (77.54%), Postives = 274/325 (84.31%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIK 60
           MGSLP+IVEDCMGVLQLYSDGTV RSD I FPFPIT D+SVVFRDVLYDA H LHLRL K
Sbjct: 1   MGSLPHIVEDCMGVLQLYSDGTVSRSDNIHFPFPITLDTSVVFRDVLYDASHALHLRLYK 60

Query: 61  PAVSLNS-------NTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYR 120
           P  S +S       N KLP+LFFFHGGGFCVGSRSW N HNCCVRLALGL+ALVI PDYR
Sbjct: 61  PPSSSSSSSSSPTTNKKLPILFFFHGGGFCVGSRSWPNSHNCCVRLALGLDALVIAPDYR 120

Query: 121 LAPENRLPAAVEDGEKAIEWVRKGE--DAWIEEGADLERVFVMGDSSGGNIAHHLAVGFG 180
           LAPE+RLPAAVEDG KAIEWVRK    D WIEE  DL+RVFVMGDSSGGNIAHHLAV  G
Sbjct: 121 LAPEHRLPAAVEDGVKAIEWVRKAGKLDEWIEESGDLKRVFVMGDSSGGNIAHHLAVRIG 180

Query: 181 NDRGRFGVRIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDHPL 240
            +  +FGV  RGFVLMAPFFGGV RTKSEEGP E   +L+ALDRFWRLSLP+GEDRDHPL
Sbjct: 181 IENEKFGV--RGFVLMAPFFGGVRRTKSEEGPAEQLFDLEALDRFWRLSLPIGEDRDHPL 240

Query: 241 ANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFF 300
           ANPFG SS SLE V LEPI+V+VG +E+LKDR ++YAKTLSQLG RIEYVEFEGKQHGFF
Sbjct: 241 ANPFGASSMSLEEVKLEPILVIVGGDEMLKDRAENYAKTLSQLGKRIEYVEFEGKQHGFF 300

Query: 301 TSSQDTQLGDQVLAIIKRFMLQNSL 317
           T+SQD QLG QV+AIIK+FML NS+
Sbjct: 301 TNSQDPQLGHQVIAIIKKFMLHNSV 323

BLAST of Carg05330 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.0e-95
Identity = 173/323 (53.56%), Postives = 227/323 (70.28%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRF---PFPITDDSSVVFRDVLYDAVHGLHLR 60
           +G  P + EDCMG+LQL S+GTV RS+ I       P  ++ +V+F+D +Y   + LHLR
Sbjct: 4   LGEEPQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLR 63

Query: 61  LIKPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPE 120
           L KP +S ++ T LPV+ FFHGGGFC GSRSW + HN C+ LA  L ALV+ PDYRLAPE
Sbjct: 64  LYKP-ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPE 123

Query: 121 NRLPAAVEDGEKAIEW-----VRKGEDAWIEEGADL--ERVFVMGDSSGGNIAHHLAVGF 180
           +RLPAA ED E  + W     V  G + W E+G D+  +RVFV+GDSSGGNIAH LAV F
Sbjct: 124 HRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRF 183

Query: 181 GNDRGRF-GVRIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDH 240
           G+       VR+RG+VLM PFFGG  RT SE GP E  L+LD LD+FWRLSLP G  RDH
Sbjct: 184 GSGSIELTPVRVRGYVLMGPFFGGEERTNSENGPSEALLSLDLLDKFWRLSLPNGATRDH 243

Query: 241 PLANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQL-GHRIEYVEFEGKQH 300
            +ANPFGP+S +LE ++LEP++V+VG +ELL+DR + YA  L ++ G R++Y+EFE K+H
Sbjct: 244 HMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEH 303

Query: 301 GFFTSSQDTQLGDQVLAIIKRFM 312
           GF+++   ++  +QVL II  FM
Sbjct: 304 GFYSNYPSSEAAEQVLRIIGDFM 325

BLAST of Carg05330 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.0e-95
Identity = 173/323 (53.56%), Postives = 227/323 (70.28%), Query Frame = 0

Query: 1   MGSLPYIVEDCMGVLQLYSDGTVFRSDEIRF---PFPITDDSSVVFRDVLYDAVHGLHLR 60
           +G  P + EDCMG+LQL S+GTV RS+ I       P  ++ +V+F+D +Y   + LHLR
Sbjct: 4   LGEEPQVAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLR 63

Query: 61  LIKPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPE 120
           L KP +S ++ T LPV+ FFHGGGFC GSRSW + HN C+ LA  L ALV+ PDYRLAPE
Sbjct: 64  LYKP-ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPE 123

Query: 121 NRLPAAVEDGEKAIEW-----VRKGEDAWIEEGADL--ERVFVMGDSSGGNIAHHLAVGF 180
           +RLPAA ED E  + W     V  G + W E+G D+  +RVFV+GDSSGGNIAH LAV F
Sbjct: 124 HRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRF 183

Query: 181 GNDRGRF-GVRIRGFVLMAPFFGGVVRTKSEEGPPEDFLNLDALDRFWRLSLPLGEDRDH 240
           G+       VR+RG+VLM PFFGG  RT SE GP E  L+LD LD+FWRLSLP G  RDH
Sbjct: 184 GSGSIELTPVRVRGYVLMGPFFGGEERTNSENGPSEALLSLDLLDKFWRLSLPNGATRDH 243

Query: 241 PLANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQL-GHRIEYVEFEGKQH 300
            +ANPFGP+S +LE ++LEP++V+VG +ELL+DR + YA  L ++ G R++Y+EFE K+H
Sbjct: 244 HMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEH 303

Query: 301 GFFTSSQDTQLGDQVLAIIKRFM 312
           GF+++   ++  +QVL II  FM
Sbjct: 304 GFYSNYPSSEAAEQVLRIIGDFM 325

BLAST of Carg05330 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 174.9 bits (442), Expect = 1.0e-43
Identity = 102/308 (33.12%), Postives = 159/308 (51.62%), Query Frame = 0

Query: 7   IVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDSSVVFRDVLYDAVHGLHLRLIKPAVSLN 66
           +VE+  G++++++DG V R   +    P    SS      +  +        I  A + +
Sbjct: 28  VVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDIKLSNDTWTRVYIPDAAAAS 87

Query: 67  SNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRLPAAVED 126
            +  LP+L +FHGGGFCVGS +W+  H+    LA+    +++  +YRLAPE+RLPAA +D
Sbjct: 88  PSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHRLPAAYDD 147

Query: 127 GEKAIEWVRK-------GEDAWIEEGADLERVFVMGDSSGGNIAHHLAVG-FGNDRGRFG 186
           G   + W+ K       G  +W+ +  +L  VF+ GDS+G NIA+ +AV    + +    
Sbjct: 148 GVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVRIMASGKYANT 207

Query: 187 VRIRGFVLMAPFFGGVVRTKSEE---GPPEDFLNLDALDRFWRLSLPLGEDRDHPLANPF 246
           + ++G +L+ PFFGG  RT SE+         L L A D +WRL+LP G  RDHP  NP 
Sbjct: 208 LHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGASRDHPWCNPL 267

Query: 247 GPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEFEGKQHGFFTSSQ 304
             S+    G  L   MV + E ++LK+R     K +   G R+E +   G  H F     
Sbjct: 268 MSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGGVGHAFHILDN 327

BLAST of Carg05330 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 5.8e-42
Identity = 102/267 (38.20%), Postives = 146/267 (54.68%), Query Frame = 0

Query: 7   IVEDCMGVLQLYSDGTVFRSDEIRFPFPITDDS-----SVVFRDVLYDAVHGLHLRLIKP 66
           +V++  G++++Y DG V RS  +    P  D S      V   DV+ D +  +  RL  P
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLL----PCVDPSLPLELGVTCSDVVIDKLTNVWARLYVP 82

Query: 67  AVSLNSN-TKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEALVIVPDYRLAPENRL 126
             +  S+ +KLP++ +FHGGGFCVGS SW   H    RL+     LV+  +YRLAPEN L
Sbjct: 83  MTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAPENPL 142

Query: 127 PAAVEDGEKAIEWVRK--GEDAWIEEGADLERVFVMGDSSGGNIAHHLAVGFGNDRGRFG 186
           PAA EDG  AI W+ K   ++ W ++  D  R+F+ GDS+GGNIA  +A    +      
Sbjct: 143 PAAYEDGVNAILWLNKARNDNLWAKQ-CDFGRIFLAGDSAGGNIAQQVAARLASPED-LA 202

Query: 187 VRIRGFVLMAPFFGGVVRTKSEEGPPED---FLNLDALDRFWRLSLPLGEDRDHPLANPF 246
           ++I G +L+ PF+ G  RT+SE     D    L L + D +WR+SLP G +R+HP   P 
Sbjct: 203 LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANREHPYCKPV 262

Query: 247 GPSSKSLEGVNLEPIMVMVGENELLKD 263
               KS     +   +V V E +LL D
Sbjct: 263 KMIIKS---STVTRTLVCVAEMDLLMD 280

BLAST of Carg05330 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 165.6 bits (418), Expect = 6.4e-41
Identity = 97/248 (39.11%), Postives = 139/248 (56.05%), Query Frame = 0

Query: 46  VLYDAVHGLHLRLIKPAVSLNSNTKLPVLFFFHGGGFCVGSRSWANPHNCCVRLALGLEA 105
           +L+   HG  L L KP   L++   +PVL FFHGG F   S + A     C RL      
Sbjct: 85  LLHQTRHGT-LELTKP---LSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGV 144

Query: 106 LVIVPDYRLAPENRLPAAVEDGEKAIEWVRKGEDAWIEEGADLE-RVFVMGDSSGGNIAH 165
           +V+  DYR +PE+R P A +DG  A+ WV+     W++ G D    V++ GDSSGGNIAH
Sbjct: 145 VVVSVDYRRSPEHRYPCAYDDGWNALNWVK--SRVWLQSGKDSNVYVYLAGDSSGGNIAH 204

Query: 166 HLAVGFGNDRGRFGVRIRGFVLMAPFFGGVVRTKSEEG-PPEDFLNLDALDRFWRLSLPL 225
           ++AV   N+    GV++ G +L+ P FGG  RT+SE+    + F+ +   D +WR  LP 
Sbjct: 205 NVAVRATNE----GVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPE 264

Query: 226 GEDRDHPLANPFGPSSKSLEGVNLEPIMVMVGENELLKDRVQHYAKTLSQLGHRIEYVEF 285
           GEDRDHP  NPFGP  +SL+GVN    +V+V   +L++D    Y   L + G  +  +  
Sbjct: 265 GEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYL 322

Query: 286 EGKQHGFF 292
           +    GF+
Sbjct: 325 KQATIGFY 322

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027165.18.3e-184100.00putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022963184.12.4e-18399.68probable carboxylesterase 15 [Cucurbita moschata][more]
XP_023517355.14.1e-18399.68probable carboxylesterase 15 [Cucurbita pepo subsp. pepo][more]
XP_023003802.16.1e-17996.84probable carboxylesterase 15 [Cucurbita maxima][more]
KAG6595149.11.3e-176100.00putative carboxylesterase 15, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9FG132.8e-9453.56Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q9LFR71.5e-4233.12Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9SX258.1e-4138.20Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LYC19.0e-4039.11Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q9LVB81.5e-3934.97Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HJC41.2e-18399.68probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111463468 PE=4 ... [more]
A0A6J1KNM63.0e-17996.84probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111497276 PE=4 SV... [more]
A0A6J1BWM51.6e-14579.81probable carboxylesterase 15 OS=Momordica charantia OX=3673 GN=LOC111005401 PE=4... [more]
A0A5D3C8G71.8e-13977.54Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CHD31.8e-13977.54probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103500946 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G06570.12.0e-9553.56alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.22.0e-9553.56alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.11.0e-4333.12carboxyesterase 17 [more]
AT1G68620.15.8e-4238.20alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.16.4e-4139.11alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 74..291
e-value: 5.9E-57
score: 192.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 14..315
e-value: 1.7E-76
score: 259.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 4..310
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 4..312
NoneNo IPR availablePANTHERPTHR23024:SF406CARBOXYLESTERASE 15-RELATEDcoord: 4..312

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05330-RACarg05330-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity