Carg05157 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05157
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nitrile-specifier protein 5
Location	Carg_Chr07: 3227336 .. 3228566 (-)
RNA-Seq Expression	Carg05157
Synteny	Carg05157

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGACGCCATTGCTATCTGCCGATTGAGCTCTGGACTTTCCGCAAATGGCTCCGATGAACGGAAAATGGACGAAGGTCAGTCCCTTAAGCTAATTAAGCTCATTGATTCAATCTTCAATTCACCATTTTATGAATTCATCTTCATAATTTCAACTTCTATTGCCACAAATTTCATTTACTTTTCGAAATCTTGATCAATTTGTTGCAGATCGATCAAAACGGCGCAGGACCAGGCGCAAGAAGCTCTCACGCGATCACAGTCGTAGGGAAAAAGGCCTACGCCTTCGGCGGCGAGTTTTCGCCGCGGATCCCCGTCGACAACGAACTCCACGTCTTCGATCTCGACGAACACACTTGGACGGTCGCTTCTGTCTCCGGCGACGTTCCTCCCCCGCGTGTTGGCGTCACGATGGCTGCCGTCGGCCAAATCGTATACGTCTTCGGCGGTAGGGATTTGGAGCATAAGGAACTTAACGAACTCTACTCTTTCGACACATCGATCAAAAAATGGACGCTGCTCTCTGGCGGCGAGTCCGGGCCGCCTCACCGGAGCTACCACTCCACCACCGCTGATGACCGCCGCGTCTACATCTTCGGCGGATGCGGCAACTCCGGCCGCCTCAATGACTTGTGGGCTTACGATACGATAGAGGGAAAATGGATCGAGTATCCGTCGGCAGGGGAGGGGTTAAAGGGCCGAGGTGGGCCGGGCTTAGCTGTGGCCCAAGGGAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTACACTACTTCGATCCGGCCCAAAACAAATGGGCCCAAGTTGAGACGACAGGGGAAAGACCCACGGCTCGGAGCGTGTTCTCCACGTGCGCGATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGACATGGGGCATTTGGGCGCCGGAAAGTTCGCGGGAGAGGTGTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTAGAAGATGGGCTGGGCCTGGAGAATCATCCTGGGCCGAGAGGGTGGAGTGCGTTTTCAGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTAGTTTATGGGGGAAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTCACTCCTGTGTGAGCGAGAATGGTAAATGTAGTTGACGGTCCTGATTTGATCGATTCCTATGATGGGTGTGATTATGTGTATATTTCGAGTTTTT

mRNA sequence

CGACGCCATTGCTATCTGCCGATTGAGCTCTGGACTTTCCGCAAATGGCTCCGATGAACGGAAAATGGACGAAGATCGATCAAAACGGCGCAGGACCAGGCGCAAGAAGCTCTCACGCGATCACAGTCGTAGGGAAAAAGGCCTACGCCTTCGGCGGCGAGTTTTCGCCGCGGATCCCCGTCGACAACGAACTCCACGTCTTCGATCTCGACGAACACACTTGGACGGTCGCTTCTGTCTCCGGCGACGTTCCTCCCCCGCGTGTTGGCGTCACGATGGCTGCCGTCGGCCAAATCGTATACGTCTTCGGCGGTAGGGATTTGGAGCATAAGGAACTTAACGAACTCTACTCTTTCGACACATCGATCAAAAAATGGACGCTGCTCTCTGGCGGCGAGTCCGGGCCGCCTCACCGGAGCTACCACTCCACCACCGCTGATGACCGCCGCGTCTACATCTTCGGCGGATGCGGCAACTCCGGCCGCCTCAATGACTTGTGGGCTTACGATACGATAGAGGGAAAATGGATCGAGTATCCGTCGGCAGGGGAGGGGTTAAAGGGCCGAGGTGGGCCGGGCTTAGCTGTGGCCCAAGGGAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTACACTACTTCGATCCGGCCCAAAACAAATGGGCCCAAGTTGAGACGACAGGGGAAAGACCCACGGCTCGGAGCGTGTTCTCCACGTGCGCGATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGACATGGGGCATTTGGGCGCCGGAAAGTTCGCGGGAGAGGTGTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTAGAAGATGGGCTGGGCCTGGAGAATCATCCTGGGCCGAGAGGGTGGAGTGCGTTTTCAGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTAGTTTATGGGGGAAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTCACTCCTGTGTGAGCGAGAATGGTAAATGTAGTTGACGGTCCTGATTTGATCGATTCCTATGATGGGTGTGATTATGTGTATATTTCGAGTTTTT

Coding sequence (CDS)

ATGGCTCCGATGAACGGAAAATGGACGAAGATCGATCAAAACGGCGCAGGACCAGGCGCAAGAAGCTCTCACGCGATCACAGTCGTAGGGAAAAAGGCCTACGCCTTCGGCGGCGAGTTTTCGCCGCGGATCCCCGTCGACAACGAACTCCACGTCTTCGATCTCGACGAACACACTTGGACGGTCGCTTCTGTCTCCGGCGACGTTCCTCCCCCGCGTGTTGGCGTCACGATGGCTGCCGTCGGCCAAATCGTATACGTCTTCGGCGGTAGGGATTTGGAGCATAAGGAACTTAACGAACTCTACTCTTTCGACACATCGATCAAAAAATGGACGCTGCTCTCTGGCGGCGAGTCCGGGCCGCCTCACCGGAGCTACCACTCCACCACCGCTGATGACCGCCGCGTCTACATCTTCGGCGGATGCGGCAACTCCGGCCGCCTCAATGACTTGTGGGCTTACGATACGATAGAGGGAAAATGGATCGAGTATCCGTCGGCAGGGGAGGGGTTAAAGGGCCGAGGTGGGCCGGGCTTAGCTGTGGCCCAAGGGAAAATATGGGTCGTGTACGGATTCGCGGGTATGGAAATGGACGACGTACACTACTTCGATCCGGCCCAAAACAAATGGGCCCAAGTTGAGACGACAGGGGAAAGACCCACGGCTCGGAGCGTGTTCTCCACGTGCGCGATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGACATGGGGCATTTGGGCGCCGGAAAGTTCGCGGGAGAGGTGTATGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTAGAAGATGGGCTGGGCCTGGAGAATCATCCTGGGCCGAGAGGGTGGAGTGCGTTTTCAGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTAGTTTATGGGGGAAATTCGCCGAGCAATGATCGGCTGGGGGATATCTACTTTTTCACTCCTGTGTGA

Protein sequence

MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVYGGNSPSNDRLGDIYFFTPV

Homology

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BLAST of Carg05157 vs. NCBI nr
Match: KAG7026998.1 (Nitrile-specifier protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691.0 bits (1782), Expect = 4.8e-195
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

[Close]

BLAST of Carg05157 vs. NCBI nr
Match: XP_022947523.1 (nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 689.1 bits (1777), Expect = 1.8e-194
Identity = 322/323 (99.69%), Postives = 322/323 (99.69%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

[Close]

BLAST of Carg05157 vs. NCBI nr
Match: XP_023533621.1 (nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 680.2 bits (1754), Expect = 8.4e-192
Identity = 317/323 (98.14%), Postives = 319/323 (98.76%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAIT+VGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYS DTSI KWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSLDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGW AFSVGRKG ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGRELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

[Close]

BLAST of Carg05157 vs. NCBI nr
Match: XP_022971053.1 (nitrile-specifier protein 5 [Cucurbita maxima])

HSP 1 Score: 675.6 bits (1742), Expect = 2.1e-190
Identity = 314/323 (97.21%), Postives = 318/323 (98.45%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAIT+VGKKAYAFGGEFSPRIPVDN+LHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTM AVGQIVYVFGGRDLEHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTI+GKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPT RSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

[Close]

BLAST of Carg05157 vs. NCBI nr
Match: XP_008464157.1 (PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >KAA0060815.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa] >TYK08820.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 621.3 bits (1601), Expect = 4.6e-174
Identity = 281/322 (87.27%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAP 322

[Close]

BLAST of Carg05157 vs. ExPASy Swiss-Prot
Match: Q93XW5 (Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 3.6e-105
Identity = 173/320 (54.06%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           M P+  KW K+ Q GAGPGARSSHA+TVVG K Y FGGE  P I +DN+L+VFDL+   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           ++A  +G+ P P  GV+M  +G  +YV+GGRD + +  N L+S+DT   +W LL+  E G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD ++ KW+EYP+AGE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           V +G+IWV++GF G E+ D+H FD A  +W  VETTG+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           E +P ++ H+GAGK +GEVY LDTE L W R+  G   E  P  RGW AF+   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

[Close]

BLAST of Carg05157 vs. ExPASy Swiss-Prot
Match: O04316 (Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 4.0e-96
Identity = 153/318 (48.11%), Postives = 217/318 (68.24%), Query Frame = 0


Query: 4   MNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVA 63
           ++GKWTK+D+NG GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFD++  TW+++
Sbjct: 298 LHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIESRTWSIS 357

Query: 64  SVSGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPP 123
             +GD+P    +GV M ++G  +YVFGGRD   ++ N  YSFDT+  +W LL+  E GP 
Sbjct: 358 PATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPT 417

Query: 124 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 183
            RS+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V 
Sbjct: 418 PRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVV 477

Query: 184 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 243
           QGK+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ A+GK++ I+GGE+
Sbjct: 478 QGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIVIFGGEI 537

Query: 244 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLL 303
               + H+G G+     + LDTE L+W R++   G E  P  RGW+A +    GG+ GL+
Sbjct: 538 AMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIGGKKGLV 597

Query: 304 VYGGNSPSNDRLGDIYFF 321
           ++GG +P+NDR  D++F+
Sbjct: 598 MHGGKAPTNDRFDDLFFY 614

[Close]

BLAST of Carg05157 vs. ExPASy Swiss-Prot
Match: Q9SDM9 (Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2)

HSP 1 Score: 346.7 bits (888), Expect = 2.9e-94
Identity = 154/316 (48.73%), Postives = 212/316 (67.09%), Query Frame = 0


Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           GKW K++Q G GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD   ++ N  YSFDT+  +W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R++   G E  P  RGW+A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of Carg05157 vs. ExPASy Swiss-Prot
Match: O49326 (Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 5.1e-91
Identity = 154/319 (48.28%), Postives = 213/319 (66.77%), Query Frame = 0


Query: 4   MNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVA 63
           + GKW K++Q G GPG R SH I  VG K ++FGGE +P  P+D  L+VFDL+  TW+++
Sbjct: 150 LRGKWIKVEQKGEGPGPRCSHDIAQVGNKIFSFGGELTPNQPIDKHLYVFDLETRTWSIS 209

Query: 64  SVSGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPP 123
             +GDVP    +GV M ++G  +YVFGGRD   K  N  YSFDT+  +W LL+  E GP 
Sbjct: 210 PATGDVPNLSCLGVRMVSIGSSLYVFGGRDASRK-YNGFYSFDTTKNEWKLLTPVEQGPT 269

Query: 124 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 183
            RS+HS TAD+  VY+FGG   + RL  L AY+ ++ KW++  + G     RGG GL V 
Sbjct: 270 PRSFHSMTADENNVYVFGGVSATVRLKTLDAYNIVDHKWVQCSTPGGSCSVRGGAGLEVV 329

Query: 184 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 243
           QGK+WVVYGF G E+DDVH +DPAQ+KW QVET GE+P ARSVF++  +GK++ ++GGE+
Sbjct: 330 QGKVWVVYGFNGCEVDDVHCYDPAQDKWTQVETFGEKPCARSVFASAVVGKHILVFGGEI 389

Query: 244 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENH-PGPRGWSAFSVGRKGGELGL 303
                 H G G+ +G  + LDTE L+W ++ D LG E   P  RGWSA + G   G+ GL
Sbjct: 390 AMDPKAHEGPGQLSGGTFALDTETLKWEKL-DKLGEEEETPSIRGWSASTTGTIDGKKGL 449

Query: 304 LVYGGNSPSNDRLGDIYFF 321
           +++GG + +NDR GD++F+
Sbjct: 450 VMFGGKAQTNDRFGDLFFY 466

[Close]

BLAST of Carg05157 vs. ExPASy Swiss-Prot
Match: O04318 (Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 2.1e-89
Identity = 147/316 (46.52%), Postives = 205/316 (64.87%), Query Frame = 0


Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           G W K++QNG GPG R SH I  VG K Y+FGGE  P  P+D  L+VFDL+  TW++A  
Sbjct: 151 GNWIKVEQNGEGPGLRCSHGIAQVGNKIYSFGGELIPNQPIDKHLYVFDLETRTWSIAPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +Y FGGRD   ++ N  YSFDT+  +W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYTFGGRDFS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG G   R+  L +Y+ ++  W    + G+    RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVGAMDRIKTLDSYNIVDKTWFHCSNPGDSFSIRGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+W+VYGF G E+DDVH++DPA++KW QVET G +P  RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWIVYGFNGCEVDDVHFYDPAEDKWTQVETFGVKPNERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
               H+G G+     + LDTE L+W R++     E  P  RGW+A + G   G+ GL+++
Sbjct: 391 DPRAHVGPGQLIDGTFALDTETLQWERLDK---FEGTPSSRGWTASTTGTIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 462

[Close]

BLAST of Carg05157 vs. ExPASy TrEMBL
Match: A0A6J1G6U4 (nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 8.8e-195
Identity = 322/323 (99.69%), Postives = 322/323 (99.69%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

[Close]

BLAST of Carg05157 vs. ExPASy TrEMBL
Match: A0A6J1I5Q1 (nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.0e-190
Identity = 314/323 (97.21%), Postives = 318/323 (98.45%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAPMNGKWTKIDQNGAGPGARSSHAIT+VGKKAYAFGGEFSPRIPVDN+LHVFDLDEHTW
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TVASVSGDVPPPRVGVTM AVGQIVYVFGGRDLEHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTI+GKWIEYPSAGEGLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPT RSVFSTCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTPV 324
           LLVYGGNSPSNDRLGDIYFFTPV
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTPV 323

[Close]

BLAST of Carg05157 vs. ExPASy TrEMBL
Match: A0A1S3CLA8 (nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.2e-174
Identity = 281/322 (87.27%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAP 322

[Close]

BLAST of Carg05157 vs. ExPASy TrEMBL
Match: A0A5D3CBW1 (Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00270 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.2e-174
Identity = 281/322 (87.27%), Postives = 300/322 (93.17%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSI KWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAP 322

[Close]

BLAST of Carg05157 vs. ExPASy TrEMBL
Match: A0A0A0KDY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.7e-172
Identity = 277/322 (86.02%), Postives = 296/322 (91.93%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           MAP  G W KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LH+FDL +H W
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           TV + SGD+PPPRVGVTMA+VGQI+YVFGGRD EHKELNELYSFDTSI KWTLLSGG+ G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
           PP RSYHSTTADDRR+YIFGGCGNSGRLNDLWAYDTI+GKWIEYP+AGEGLKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYF P QNKW QVET GERPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           EVDPSD+GHLGAGKF GEVYVLDTEALEWSRVEDGLGLE+HPGPRGW AF+VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFTP 323
           LLVYGGNSPSNDRLGDI+FF P
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAP 322

[Close]

BLAST of Carg05157 vs. TAIR 10
Match: AT3G07720.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 485.0 bits (1247), Expect = 4.8e-137
Identity = 213/320 (66.56%), Postives = 253/320 (79.06%), Query Frame = 0


Query: 3   PMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTV 62
           PM GKW ++ Q G GPGARSSHAI +VG K YAFGGEF PR+PVDN+L+VFDL+  TW++
Sbjct: 5   PMEGKWVQLKQKGTGPGARSSHAIALVGNKMYAFGGEFQPRVPVDNQLYVFDLETQTWSI 64

Query: 63  ASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPP 122
              SGD PPPRVGV MAAVG I+Y FGGRD  H+ELNELY F+T   +W LLS GE+GP 
Sbjct: 65  QEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTHQELNELYCFNTLTNQWKLLSSGETGPQ 124

Query: 123 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 182
           +RSYHS TAD + VY+FGGCG  GRLNDLWAY+ ++ KWI++PS GE  +GRGGPGL V 
Sbjct: 125 NRSYHSITADSQNVYVFGGCGVDGRLNDLWAYNVVDQKWIKFPSPGEACRGRGGPGLEVV 184

Query: 183 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 242
           QGKIWVVYGFAG E DDVH FD A+ +W +VET GE+P+ARSVFST  +GK + I GGE+
Sbjct: 185 QGKIWVVYGFAGEEADDVHCFDIAKGEWKEVETKGEKPSARSVFSTAVVGKQILISGGEI 244

Query: 243 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLL 302
           DPSD+GH+GAG F G+ Y LDTE LEW + EDG+G   HPGPRGW AF+ G + G+ GLL
Sbjct: 245 DPSDLGHMGAGCFTGDAYGLDTETLEWRKWEDGVGSVEHPGPRGWCAFAAGSRDGKQGLL 304

Query: 303 VYGGNSPSNDRLGDIYFFTP 323
           VYGGNSPSNDRL DI+FFTP
Sbjct: 305 VYGGNSPSNDRLDDIFFFTP 324

[Close]

BLAST of Carg05157 vs. TAIR 10
Match: AT5G48180.1 (nitrile specifier protein 5 )

HSP 1 Score: 382.9 bits (982), Expect = 2.6e-106
Identity = 173/320 (54.06%), Postives = 223/320 (69.69%), Query Frame = 0


Query: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60
           M P+  KW K+ Q GAGPGARSSHA+TVVG K Y FGGE  P I +DN+L+VFDL+   W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESG 120
           ++A  +G+ P P  GV+M  +G  +YV+GGRD + +  N L+S+DT   +W LL+  E G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD ++ KW+EYP+AGE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240
           V +G+IWV++GF G E+ D+H FD A  +W  VETTG+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300
           E +P ++ H+GAGK +GEVY LDTE L W R+  G   E  P  RGW AF+   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

[Close]

BLAST of Carg05157 vs. TAIR 10
Match: AT3G16410.1 (nitrile specifier protein 4 )

HSP 1 Score: 352.8 bits (904), Expect = 2.9e-97
Identity = 153/318 (48.11%), Postives = 217/318 (68.24%), Query Frame = 0


Query: 4   MNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVA 63
           ++GKWTK+D+NG GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFD++  TW+++
Sbjct: 298 LHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIESRTWSIS 357

Query: 64  SVSGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPP 123
             +GD+P    +GV M ++G  +YVFGGRD   ++ N  YSFDT+  +W LL+  E GP 
Sbjct: 358 PATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPT 417

Query: 124 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVA 183
            RS+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V 
Sbjct: 418 PRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVV 477

Query: 184 QGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEV 243
           QGK+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ A+GK++ I+GGE+
Sbjct: 478 QGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIVIFGGEI 537

Query: 244 DPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLL 303
               + H+G G+     + LDTE L+W R++   G E  P  RGW+A +    GG+ GL+
Sbjct: 538 AMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIGGKKGLV 597

Query: 304 VYGGNSPSNDRLGDIYFF 321
           ++GG +P+NDR  D++F+
Sbjct: 598 MHGGKAPTNDRFDDLFFY 614

[Close]

BLAST of Carg05157 vs. TAIR 10
Match: AT3G16400.1 (nitrile specifier protein 1 )

HSP 1 Score: 346.7 bits (888), Expect = 2.1e-95
Identity = 154/316 (48.73%), Postives = 212/316 (67.09%), Query Frame = 0


Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           GKW K++Q G GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD   ++ N  YSFDT+  +W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R++   G E  P  RGW+A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of Carg05157 vs. TAIR 10
Match: AT3G16400.2 (nitrile specifier protein 1 )

HSP 1 Score: 346.7 bits (888), Expect = 2.1e-95
Identity = 154/316 (48.73%), Postives = 212/316 (67.09%), Query Frame = 0


Query: 6   GKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTWTVASV 65
           GKW K++Q G GPG R SH I  VG K Y+FGGEF+P  P+D  L+VFDL+  TW+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSIKKWTLLSGGESGPPHR 125
           +GDVP    +GV M +VG  +YVFGGRD   ++ N  YSFDT+  +W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLAVAQG 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ ++ KW    + G+ L  RGG GL V QG
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF++ AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R++   G E  P  RGW+A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

The following BLAST results are available for this feature:

BLAST of Carg05157 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026998.1	4.8e-195	100.00	Nitrile-specifier protein 5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022947523.1	1.8e-194	99.69	nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier...	[more]
XP_023533621.1	8.4e-192	98.14	nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022971053.1	2.1e-190	97.21	nitrile-specifier protein 5 [Cucurbita maxima]	[more]
XP_008464157.1	4.6e-174	87.27	PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED:...	[more]

BLAST of Carg05157 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93XW5	3.6e-105	54.06	Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1	[more]
O04316	4.0e-96	48.11	Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1	[more]
Q9SDM9	2.9e-94	48.73	Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2	[more]
O49326	5.1e-91	48.28	Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1	[more]
O04318	2.1e-89	46.52	Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1	[more]

BLAST of Carg05157 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G6U4	8.8e-195	99.69	nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 S...	[more]
A0A6J1I5Q1	1.0e-190	97.21	nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=...	[more]
A0A1S3CLA8	2.2e-174	87.27	nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1	[more]
A0A5D3CBW1	2.2e-174	87.27	Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A0A0KDY9	4.7e-172	86.02	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1	[more]

BLAST of Carg05157 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07720.1	4.8e-137	66.56	Galactose oxidase/kelch repeat superfamily protein	[more]
AT5G48180.1	2.6e-106	54.06	nitrile specifier protein 5	[more]
AT3G16410.1	2.9e-97	48.11	nitrile specifier protein 4	[more]
AT3G16400.1	2.1e-95	48.73	nitrile specifier protein 1	[more]
AT3G16400.2	2.1e-95	48.73	nitrile specifier protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006652	Kelch repeat type 1	SMART	SM00612	kelc_smart	coord: 185..233 e-value: 6.2 score: 4.7 coord: 135..184 e-value: 0.08 score: 21.3
IPR006652	Kelch repeat type 1	PFAM	PF01344	Kelch_1	coord: 174..213 e-value: 2.8E-6 score: 26.8
None	No IPR available	PFAM	PF13415	Kelch_3	coord: 30..80 e-value: 2.7E-10 score: 40.3
None	No IPR available	PFAM	PF13418	Kelch_4	coord: 222..274 e-value: 1.8E-5 score: 24.6 coord: 123..161 e-value: 6.4E-11 score: 42.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR47435	KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)	coord: 4..322
None	No IPR available	PANTHER	PTHR47435:SF4	KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)	coord: 4..322
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 2..193 e-value: 2.5E-41 score: 143.6
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 195..323 e-value: 1.7E-16 score: 62.0
IPR011043	Galactose oxidase/kelch, beta-propeller	SUPERFAMILY	50965	Galactose oxidase, central domain	coord: 3..318

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05157-RA	Carg05157-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0080028	nitrile biosynthetic process
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0010043	response to zinc ion
cellular_component	GO:0005634	nucleus
molecular_function	GO:0030234	enzyme regulator activity
molecular_function	GO:0005515	protein binding