Carg04883 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04883
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Plant invertase/pectin methylesterase inhibitor superfamily
Location	Carg_Chr13: 8754916 .. 8757640 (+)
RNA-Seq Expression	Carg04883
Synteny	Carg04883

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTGTTAATCTCTGCAGGTTTGTTCGTCTTTCTTTTGCGAGTCTCTGTCTCGCCGGCGTTTATGTCCGACGAGACAAAACCGTTCGTCTTCCACTCTTTTCTCTCCTCATTTCGTGGTTTCTGTCCTTACAGGTGAGGTCTAAGGGTTCGTATGAGGCTGACGTTTTTTGAAGTCGCCGGAGTTTGGTTACGCTCGCCGTAAAACCTAGTATTCTTCATCTGTGTGTTTGTAACTTATTTGCTAAATTTCGTCTTCCACGCTCTTCTCTCTTCATTTTGTGGTTTCTGTCCTTACAGGTGAGATTCGTTGGAGGCTGTTTTCCAAGTCGCCGGAGTACTCGCCGGAAAACTTCGGTATTCTCCATCTCTGTGTTTGTAACTTCTTTGCTAACTTTCGTCTTCAACTCTTTTCTCTCTTTATTTCGTGGTTTCTGTAGTTACAGGTGAGGTCTAAGGGTTTGGAGACAGACTTATTCGAAGTCGCCGGAGTTTGGGTTACGGTCGCCGGAAAACTTTGGTATTATCCATCTTTTTCAATTTTTCTTTCTTTCTTCGTCTTTGTCTCTTTGTCTCTTTGTCTCTTTGTCTCGGTAACTTTTAAATGAAAATGACATCTAACCTCTCGTTTACAAATTTAGTGATGTTTAACTTTTGGGTTTACATATTTAGCTGACAGTGACATTTAACATCTGAATAGACAGTGACAGTGACATGTAACATTTTCTTTGCTATAAGAAGAAATATACATTTTTTCCTCTCTTCCATTTTCGTCTCTTCTCTTTCCCATATAAATGAACTGAACTGATTTTAATAATAAACACACCTCAATCTCGCTCACTCTCAATCTGCTCTCCATTTTCTCTCAATCTGTTCTCCATTTTCTCTCAATCTTCTCTCAATCTCTTTCTCAATCGGCTCTTAATCTCGCTCATTTAAATTTCAATCTCTGCACCTCTATACATCTTCAAAATGTCTTGCTTTTTCATCCTCTTCTTTGTTACATTTTTCTGTACTACATCCTCCTGCATTTCTTATAATGCAACCGTCTCATTGGACGGGAAGGGAGATTATACAACAATCGGTGCCGCTATAGCAGCGGCACCGAATAACAGTGACTCCAGGTTTATCATTCATGTTAAACCTGGAATGTATTTTGAATATATAAAAATCGAATCGTCGAAAACCTTCATTACATTAAAGGGCGACGATGCGTCTACCACAACTATAGTGGGTAACAGGAGCCAGGCCACTGGCTTTGGAACTGCGGATTCAGCCACATTCGGTAAGCTAGCTGTTGTCACTTTTTTCACTTTTTTTGAAGATTTTAGAGATAAAAAAGTGTTTGACCATTTTTTTAAATTAGAAGTTAAATGTTCTAAGTCATTAATACTTTTTTAAAAAAATTAATTTATAATGTCCCTATTTGTTTCTTTAAATATTAAAATTAGTCTGTTTGCAACCAGCATTATTGAAATGGATTAAAAGTTAAAAAAGTTAAAAATTAGTCTGTTTGCAACAAGGAAACGGATTAAAAGTTAAATATTTGTGTTAATTTGCTTTTAGAATGTCTTTTAATTTGTGTTTTAACAAGTTTTTGAGAAATTTAAAATTTTTAAAAATTAATTATATTTATATTTAATACTATTGACCCCTCCTTATTTTAATCGATTTATATCATTAAAATATCTAAATATCTAAACTTTCTCACACATTTTTGAAAAAGTAATTTTTATTAAAAATAAATACAATTTTTATCTCTATTACTCATAACCACTCTTTATTTTAATAAATTTATACCATTAAAATAGGAAAATAAATACAATTTTTATCTTTAATATTAATAACCACTCTATTTTAATAAATTTATAAGATTTCTACCCGGGCGTTAGACAAGTTTTTATTTTCAATGGATTAGATATCAGTTTTTTCCTGGGTTAGGGATTAAATTTTGTTTTTTTCATTTTCTTATATTTCAATTTTATGAATGAGACCTTTTAATAATTATTATTCTCTTCAACTTTGTAGTTGTGGGTGCAAACCACTTCCTAGCTGTTTCTATTACATTTTCAAATTCTGCTGGAGCTGGACCTAAACACGGCCAAGCAGTTGCTCTTCTGAACAGCAATAATGTAAATTATACCGTATTTTACAAGTGCGTTTTTCTTGGGTATCAAGACACACTTTTCATAAATGGAAAGATGTTGTTTTTCAAAGATTGTGAAATCTACGGTACAGTAGATTTCATATTTGGAAACGCACTTGTTGTTTTTCAAGATTGTAGAATTTACGCCCGACTACTGAATAACACTGTTACTGTCACTGCACAATCTAAAGATAACATAAAACAATTGAGTGGCTTTTCGTTTCAGAATTGCAGTGTAATGGTCTCTCCTGAACTTGGGCCAGTAAATGAAACGATTGTGTTTCTTGGCCGTCCTTGGAGAGACTATTCCACTGTGATTTTCATGGAATCGTTTCTAGACTCGGTGGTGAATCCTGCCGGATGGTCAGAATGGTCCAATAGTAGTTCTCTGAAATTATTATTTTATGCTGAGTATGACAACAGAGGACCGGGGGCTAACTTATCCGGAAGAGTCAAGTGGCCGGGATTCCATGCAATAGAGAATCGAAAAGAAGCTACTCGATTCAGTGTACGGGAATTTATTAATGGGACTACATGGTTGCCAGAAACTGGTGTACCATATAGACCCGATTTGTAA

mRNA sequence

ATGGTTGTGTTAATCTCTGCAGGTTTGTTCGTCTTTCTTTTGCGAGTCTCTGTCTCGCCGGCGTTTATGTCCGACGAGACAAAACCTCGCCGGAGTTTGGTTACGCTCGCCGTAAAACCTAGTATTCTTCATCTGTGTGTGAGATTCGTTGGAGGCTGTTTTCCAAGTCGCCGGAGTACTCGCCGGAAAACTTCGTTACAGGTGAGGTCTAAGGGTTTGGAGACAGACTTATTCGAAGTCGCCGGAGTTTGGGTTACGGGAGATTATACAACAATCGGTGCCGCTATAGCAGCGGCACCGAATAACAGTGACTCCAGGTTTATCATTCATGTTAAACCTGGAATGTATTTTGAATATATAAAAATCGAATCGTCGAAAACCTTCATTACATTAAAGGGCGACGATGCGTCTACCACAACTATAGTGGGTAACAGGAGCCAGGCCACTGGCTTTGGAACTGCGGATTCAGCCACATTCGTTGTGGGTGCAAACCACTTCCTAGCTGTTTCTATTACATTTTCAAATTCTGCTGGAGCTGGACCTAAACACGGCCAAGCAGTTGCTCTTCTGAACAGCAATAATGTAAATTATACCGTATTTTACAAGTGCGTTTTTCTTGGGTATCAAGACACACTTTTCATAAATGGAAAGATGTTGTTTTTCAAAGATTGTGAAATCTACGGTACAGTAGATTTCATATTTGGAAACGCACTTGTTGTTTTTCAAGATTGTAGAATTTACGCCCGACTACTGAATAACACTGTTACTGTCACTGCACAATCTAAAGATAACATAAAACAATTGAGTGGCTTTTCGTTTCAGAATTGCAGTGTAATGGTCTCTCCTGAACTTGGGCCAGTAAATGAAACGATTGTGTTTCTTGGCCGTCCTTGGAGAGACTATTCCACTGTGATTTTCATGGAATCGTTTCTAGACTCGGTGGTGAATCCTGCCGGATGGTCAGAATGGTCCAATAGTAGTTCTCTGAAATTATTATTTTATGCTGAGTATGACAACAGAGGACCGGGGGCTAACTTATCCGGAAGAGTCAAGTGGCCGGGATTCCATGCAATAGAGAATCGAAAAGAAGCTACTCGATTCAGTGTACGGGAATTTATTAATGGGACTACATGGTTGCCAGAAACTGGTGTACCATATAGACCCGATTTGTAA

Coding sequence (CDS)

ATGGTTGTGTTAATCTCTGCAGGTTTGTTCGTCTTTCTTTTGCGAGTCTCTGTCTCGCCGGCGTTTATGTCCGACGAGACAAAACCTCGCCGGAGTTTGGTTACGCTCGCCGTAAAACCTAGTATTCTTCATCTGTGTGTGAGATTCGTTGGAGGCTGTTTTCCAAGTCGCCGGAGTACTCGCCGGAAAACTTCGTTACAGGTGAGGTCTAAGGGTTTGGAGACAGACTTATTCGAAGTCGCCGGAGTTTGGGTTACGGGAGATTATACAACAATCGGTGCCGCTATAGCAGCGGCACCGAATAACAGTGACTCCAGGTTTATCATTCATGTTAAACCTGGAATGTATTTTGAATATATAAAAATCGAATCGTCGAAAACCTTCATTACATTAAAGGGCGACGATGCGTCTACCACAACTATAGTGGGTAACAGGAGCCAGGCCACTGGCTTTGGAACTGCGGATTCAGCCACATTCGTTGTGGGTGCAAACCACTTCCTAGCTGTTTCTATTACATTTTCAAATTCTGCTGGAGCTGGACCTAAACACGGCCAAGCAGTTGCTCTTCTGAACAGCAATAATGTAAATTATACCGTATTTTACAAGTGCGTTTTTCTTGGGTATCAAGACACACTTTTCATAAATGGAAAGATGTTGTTTTTCAAAGATTGTGAAATCTACGGTACAGTAGATTTCATATTTGGAAACGCACTTGTTGTTTTTCAAGATTGTAGAATTTACGCCCGACTACTGAATAACACTGTTACTGTCACTGCACAATCTAAAGATAACATAAAACAATTGAGTGGCTTTTCGTTTCAGAATTGCAGTGTAATGGTCTCTCCTGAACTTGGGCCAGTAAATGAAACGATTGTGTTTCTTGGCCGTCCTTGGAGAGACTATTCCACTGTGATTTTCATGGAATCGTTTCTAGACTCGGTGGTGAATCCTGCCGGATGGTCAGAATGGTCCAATAGTAGTTCTCTGAAATTATTATTTTATGCTGAGTATGACAACAGAGGACCGGGGGCTAACTTATCCGGAAGAGTCAAGTGGCCGGGATTCCATGCAATAGAGAATCGAAAAGAAGCTACTCGATTCAGTGTACGGGAATTTATTAATGGGACTACATGGTTGCCAGAAACTGGTGTACCATATAGACCCGATTTGTAA

Protein sequence

MVVLISAGLFVFLLRVSVSPAFMSDETKPRRSLVTLAVKPSILHLCVRFVGGCFPSRRSTRRKTSLQVRSKGLETDLFEVAGVWVTGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNIKQLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGVPYRPDL

Homology

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BLAST of Carg04883 vs. NCBI nr
Match: KAG7019877.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 784.3 bits (2024), Expect = 5.0e-223
Identity = 390/390 (100.00%), Postives = 390/390 (100.00%), Query Frame = 0


Query: 1   MVVLISAGLFVFLLRVSVSPAFMSDETKPRRSLVTLAVKPSILHLCVRFVGGCFPSRRST 60
           MVVLISAGLFVFLLRVSVSPAFMSDETKPRRSLVTLAVKPSILHLCVRFVGGCFPSRRST
Sbjct: 1   MVVLISAGLFVFLLRVSVSPAFMSDETKPRRSLVTLAVKPSILHLCVRFVGGCFPSRRST 60

Query: 61  RRKTSLQVRSKGLETDLFEVAGVWVTGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYI 120
           RRKTSLQVRSKGLETDLFEVAGVWVTGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYI
Sbjct: 61  RRKTSLQVRSKGLETDLFEVAGVWVTGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYI 120

Query: 121 KIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAG 180
           KIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAG
Sbjct: 121 KIESSKTFITLKGDDASTTTIVGNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAG 180

Query: 181 PKHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVV 240
           PKHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVV
Sbjct: 181 PKHGQAVALLNSNNVNYTVFYKCVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVV 240

Query: 241 FQDCRIYARLLNNTVTVTAQSKDNIKQLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRD 300
           FQDCRIYARLLNNTVTVTAQSKDNIKQLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRD
Sbjct: 241 FQDCRIYARLLNNTVTVTAQSKDNIKQLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRD 300

Query: 301 YSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIEN 360
           YSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIEN
Sbjct: 301 YSTVIFMESFLDSVVNPAGWSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIEN 360

Query: 361 RKEATRFSVREFINGTTWLPETGVPYRPDL 391
           RKEATRFSVREFINGTTWLPETGVPYRPDL
Sbjct: 361 RKEATRFSVREFINGTTWLPETGVPYRPDL 390

[Close]

BLAST of Carg04883 vs. NCBI nr
Match: KAG6584282.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 605.5 bits (1560), Expect = 3.2e-169
Identity = 296/304 (97.37%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 87  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRS 146
           GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYI+IESSKTFITLKGDDASTTTIVGNRS
Sbjct: 32  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIRIESSKTFITLKGDDASTTTIVGNRS 91

Query: 147 QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVFL 206
           QATGFGTADSATFVVGANHFLAVSITFSNSAGAGP HGQAVALLNSNNVNYTVFYKCVFL
Sbjct: 92  QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPNHGQAVALLNSNNVNYTVFYKCVFL 151

Query: 207 GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNIK 266
           GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSK+N  
Sbjct: 152 GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKENQL 211

Query: 267 QLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS 326
           QLSGFSFQNCSVM SPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS
Sbjct: 212 QLSGFSFQNCSVMASPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS 271

Query: 327 SSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGVPY 386
           SSLKLLFYAEY NRGPGANLSGRVKWPGFHAIENRKEATRFSVR+FINGTTWLPETGVPY
Sbjct: 272 SSLKLLFYAEYGNRGPGANLSGRVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPY 331

Query: 387 RPDL 391
           RPDL
Sbjct: 332 RPDL 335

[Close]

BLAST of Carg04883 vs. NCBI nr
Match: KAG6584281.1 (putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 604.7 bits (1558), Expect = 5.4e-169
Identity = 296/304 (97.37%), Postives = 299/304 (98.36%), Query Frame = 0


Query: 87  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRS 146
           GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRS
Sbjct: 32  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRS 91

Query: 147 QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVFL 206
           QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNN NYTVFYKCVFL
Sbjct: 92  QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNENYTVFYKCVFL 151

Query: 207 GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNIK 266
           GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSK+  +
Sbjct: 152 GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKEYKR 211

Query: 267 QLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS 326
           QLSGFSFQNCSVM SPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS
Sbjct: 212 QLSGFSFQNCSVMASPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS 271

Query: 327 SSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGVPY 386
           SSLKLLFYAEY NRGPGANLSGRVKWPGFHAIENRKEATRFSVR+FINGTTWLPETGVPY
Sbjct: 272 SSLKLLFYAEYGNRGPGANLSGRVKWPGFHAIENRKEATRFSVRQFINGTTWLPETGVPY 331

Query: 387 RPDL 391
           RPDL
Sbjct: 332 RPDL 335

[Close]

BLAST of Carg04883 vs. NCBI nr
Match: KAG6584284.1 (putative pectinesterase/pectinesterase inhibitor 42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 405.6 bits (1041), Expect = 4.8e-109
Identity = 217/305 (71.15%), Postives = 219/305 (71.80%), Query Frame = 0


Query: 87  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRS 146
           GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDD STTTIVGNRS
Sbjct: 32  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDGSTTTIVGNRS 91

Query: 147 QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVFL 206
           QATGFGTADSATF                                               
Sbjct: 92  QATGFGTADSATF----------------------------------------------- 151

Query: 207 GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNIK 266
                                  VDFIFGNAL VFQDCRIYARLLNN VTVTAQSK+   
Sbjct: 152 -----------------------VDFIFGNALAVFQDCRIYARLLNNIVTVTAQSKEYKL 211

Query: 267 QLSGFSFQNCSVMVSPELGPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSNS 326
           QLSG SFQNCSVM SPELGPVNE IVFLG PWRDYSTVIFMESFLDSVVNPAGWSEW NS
Sbjct: 212 QLSGISFQNCSVMASPELGPVNEMIVFLGHPWRDYSTVIFMESFLDSVVNPAGWSEWFNS 266

Query: 327 SSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLP-ETGVP 386
           SSLKLLFYAEY NRG GANLS RVKW GFHAIENRKEATRFSVR+FINGTTWLP ETGVP
Sbjct: 272 SSLKLLFYAEYGNRGSGANLSERVKWSGFHAIENRKEATRFSVRQFINGTTWLPVETGVP 266

Query: 387 YRPDL 391
           YRPDL
Sbjct: 332 YRPDL 266

[Close]

BLAST of Carg04883 vs. NCBI nr
Match: XP_022148123.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 365.9 bits (938), Expect = 4.2e-97
Identity = 179/306 (58.50%), Postives = 225/306 (73.53%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           +GDY  I  AIAAAPNN   RF IHVKPG+Y E+I++   KTFI+L GDDASTT IV +R
Sbjct: 38  SGDYVHISDAIAAAPNNGSIRFYIHVKPGIYKEHIEVPELKTFISLIGDDASTTVIVDDR 97

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S   GF T  SATF V A HF+A  +TF NS  AGP++GQAVA+L  +  NYT +YKCVF
Sbjct: 98  SNHAGFSTTSSATFTVKAKHFVAQFLTFQNS--AGPENGQAVAVL--SEANYTAYYKCVF 157

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265
           +GYQDTL++ G    FK+C++YG++DFIFG+ L +FQDC +YAR+L    T+TAQSK   
Sbjct: 158 MGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGLAIFQDCNVYARILG--TTITAQSKSYK 217

Query: 266 KQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWS 325
            Q SGF+FQNC+V VSPE+  + + + V LGRPWR+YSTVIFMESFLD +V P GWSEW 
Sbjct: 218 DQRSGFTFQNCNVTVSPEIQSIKDQVTVSLGRPWRNYSTVIFMESFLDDIVEPGGWSEWP 277

Query: 326 NSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGV 385
             + + LLFYAEY+N G GA+ S RVKWPG+H + N  EA+RF+V  FI+G  WL ETG+
Sbjct: 278 R-TPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLHNEDEASRFTVEIFIDGLEWLSETGI 336

Query: 386 PYRPDL 391
           P+R  L
Sbjct: 338 PFRRGL 336

[Close]

BLAST of Carg04883 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.9e-76
Identity = 141/304 (46.38%), Postives = 202/304 (66.45%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           TG++TTI  A+ AAPN S++RFII++K G YFE +++   KT I   GD    T I  NR
Sbjct: 229 TGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANR 288

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S+  G+ T  + T  V    ++A  I+F NS  AGP   QAVA  + +  +++ FY+C F
Sbjct: 289 SRIDGWSTFQTPTVGVKGKGYIAKDISFVNS--AGPAKAQAVAFRSGS--DHSAFYRCEF 348

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLN--NTVTVTAQSKD 265
            GYQDTL+++    F+++C+IYGT+DFIFGNA VVFQ+  +YAR  N  + +  TAQS++
Sbjct: 349 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 408

Query: 266 NIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSE 325
              Q +G S  NC ++ +P+L PV E    +LGRPWR YS  + ++SF+D +++PAGW E
Sbjct: 409 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 468

Query: 326 WSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPET 385
                +L+ L+Y EY N GPGAN++ RV WPGF  IEN+ EAT+F+V  FI+G+TWL  T
Sbjct: 469 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 528

Query: 386 GVPY 387
           G+P+
Sbjct: 529 GIPF 528

[Close]

BLAST of Carg04883 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.6e-75
Identity = 141/308 (45.78%), Postives = 207/308 (67.21%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           TG+YTTIG AI+AAPN+S++RF+I++K G YFE I+I   KT I   GD    T I  NR
Sbjct: 215 TGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANR 274

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S A G+    SAT  V  + F+A  ++F N   AGP+  QAVAL +S+++  + +Y+C F
Sbjct: 275 SYADGWTAFHSATVGVRGSGFIAKDLSFVNY--AGPEKHQAVALRSSSDL--SAYYRCSF 334

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARL--LNNTVTVTAQSKD 265
             YQDT++++    F+++C+IYGTVDFIFG+A VVFQ+C +YAR    N  +  TAQ ++
Sbjct: 335 ESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRE 394

Query: 266 NIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSE 325
           N ++ +G S  +  ++ +P+L PV      +LGRPW+ YS  + M+SF+D +V+PAGW +
Sbjct: 395 NSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLK 454

Query: 326 WSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPET 385
           W +  +L+ L+Y EY N GPG+N++ RV+WPGF  IE  +EA++FSV  FI+G  WL  T
Sbjct: 455 WKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNST 514

Query: 386 GVPYRPDL 391
            +P+  DL
Sbjct: 515 RIPFTLDL 518

[Close]

BLAST of Carg04883 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.8e-74
Identity = 140/311 (45.02%), Postives = 203/311 (65.27%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSR---FIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIV 145
           TG++T I AA+AAAPNN+D     F+I+V  G+Y EYI I  +K ++ + GD  + T + 
Sbjct: 272 TGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVT 331

Query: 146 GNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYK 205
           GNRS   G+ T +SATF V A +F+AV+ITF N+  AGP+  QAVAL   +  ++++FY 
Sbjct: 332 GNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT--AGPEKHQAVAL--RSGADFSIFYS 391

Query: 206 CVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYAR--LLNNTVTVTAQ 265
           C F  YQDTL+ +    F+++C++YGTVDFIFGNA VVFQ+C +Y R  + N    +TAQ
Sbjct: 392 CSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQ 451

Query: 266 SKDNIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAG 325
            + +  Q +G S QNC++  + +L   N T+  +LGRPW++YS  ++M+S++D  V P G
Sbjct: 452 GRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVG 511

Query: 326 WSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWL 385
           W EW+   +L  L+YAEY+N GPG+N + RV WPG+H I N  +A  F+V        W+
Sbjct: 512 WREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFTVTGLFIEADWI 571

Query: 386 PETGVPYRPDL 391
            +TGVPY   L
Sbjct: 572 WKTGVPYTSGL 577

[Close]

BLAST of Carg04883 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 2.4e-71
Identity = 131/307 (42.67%), Postives = 201/307 (65.47%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSR---FIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIV 145
           TG++TTI AAIAAAPN +D     F+I+V  G+Y EY+++  +K ++ + GD  + T I 
Sbjct: 253 TGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVIT 312

Query: 146 GNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYK 205
           GNRS   G+ T +SATF++   +F+ V+IT  N+  AGP  GQAVAL +  ++  +VFY 
Sbjct: 313 GNRSVVDGWTTFNSATFILSGPNFIGVNITIRNT--AGPTKGQAVALRSGGDL--SVFYS 372

Query: 206 CVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYAR--LLNNTVTVTAQ 265
           C F  YQDTL+ +    F+++C++YGTVDFIFGNA VV Q+C +Y R      +  VTAQ
Sbjct: 373 CSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQ 432

Query: 266 SKDNIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAG 325
            + +  Q +G +   C++  + +L   N T+  +LGRPW++YS  + M++++D  + P+G
Sbjct: 433 GRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSG 492

Query: 326 WSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWL 385
           W+ WS   +L  L+YAEY+N GPG++ + RV WPG+H I N  +A+ F+V  F+ G  W+
Sbjct: 493 WNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWI 552

Query: 386 PETGVPY 387
            +TGVP+
Sbjct: 553 GQTGVPF 554

[Close]

BLAST of Carg04883 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 2.4e-71
Identity = 133/307 (43.32%), Postives = 201/307 (65.47%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSR---FIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIV 145
           TG++TTI  A+ +APN +D     F+I+V  G+Y E + I  +K ++ + GD  + T + 
Sbjct: 266 TGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVT 325

Query: 146 GNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYK 205
           GNR+   G+ T +SATF V + +F+AV++TF N+  AGP+  QAVA+ +S   + ++FY 
Sbjct: 326 GNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT--AGPEKHQAVAMRSS--ADLSIFYS 385

Query: 206 CVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYAR--LLNNTVTVTAQ 265
           C F  YQDTL+ +    F+++C+IYGTVDFIFGNA VVFQDC +Y R  + N    +TAQ
Sbjct: 386 CSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQ 445

Query: 266 SKDNIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAG 325
            + +  Q +G S  NC++  + +L   N T+  +LGRPW++YS  +FM+S++D VV P G
Sbjct: 446 GRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVG 505

Query: 326 WSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWL 385
           W EW+   +L  L+YAEY+N G G++ + RV WPG+H I N  +A  F+V  F+ G  W+
Sbjct: 506 WREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDANNFTVENFLLGDGWM 565

Query: 386 PETGVPY 387
            ++GVPY
Sbjct: 566 VQSGVPY 567

[Close]

BLAST of Carg04883 vs. ExPASy TrEMBL
Match: A0A6J1D381 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 2.1e-97
Identity = 179/306 (58.50%), Postives = 225/306 (73.53%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           +GDY  I  AIAAAPNN   RF IHVKPG+Y E+I++   KTFI+L GDDASTT IV +R
Sbjct: 38  SGDYVHISDAIAAAPNNGSIRFYIHVKPGIYKEHIEVPELKTFISLIGDDASTTVIVDDR 97

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S   GF T  SATF V A HF+A  +TF NS  AGP++GQAVA+L  +  NYT +YKCVF
Sbjct: 98  SNHAGFSTTSSATFTVKAKHFVAQFLTFQNS--AGPENGQAVAVL--SEANYTAYYKCVF 157

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265
           +GYQDTL++ G    FK+C++YG++DFIFG+ L +FQDC +YAR+L    T+TAQSK   
Sbjct: 158 MGYQDTLYVRGAFQLFKECDVYGSIDFIFGDGLAIFQDCNVYARILG--TTITAQSKSYK 217

Query: 266 KQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWS 325
            Q SGF+FQNC+V VSPE+  + + + V LGRPWR+YSTVIFMESFLD +V P GWSEW 
Sbjct: 218 DQRSGFTFQNCNVTVSPEIQSIKDQVTVSLGRPWRNYSTVIFMESFLDDIVEPGGWSEWP 277

Query: 326 NSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGV 385
             + + LLFYAEY+N G GA+ S RVKWPG+H + N  EA+RF+V  FI+G  WL ETG+
Sbjct: 278 R-TPVYLLFYAEYNNSGSGADTSQRVKWPGYHVLHNEDEASRFTVEIFIDGLEWLSETGI 336

Query: 386 PYRPDL 391
           P+R  L
Sbjct: 338 PFRRGL 336

[Close]

BLAST of Carg04883 vs. ExPASy TrEMBL
Match: A0A6J1KPG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.1e-95
Identity = 181/306 (59.15%), Postives = 225/306 (73.53%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           TG++  I  AI AAPN + +RF IH+KPG+Y E+I+I S KTFI+L GD+ASTT IV NR
Sbjct: 36  TGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEIPSQKTFISLIGDNASTTIIVDNR 95

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S+ATGF TA+SAT  V   +F+A  +TF NS  AGPK GQAVA+L  +   +T +YKCVF
Sbjct: 96  SKATGFSTANSATLTVNGANFMAKFLTFQNS--AGPKMGQAVAVL--DQAKHTAYYKCVF 155

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265
           LGYQDTL+      FFK+C+IYGTVDFIFGN LVVFQDC IYARL++   TVTAQSK N+
Sbjct: 156 LGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLVDIQSTVTAQSKANL 215

Query: 266 KQLSGFSFQNCSVMVSPELGPV-NETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWS 325
            + SGF FQNC+V VSPE+    ++ +VFLGRPWR YS V F+ESFLD+VV P GW EW 
Sbjct: 216 DEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESFLDNVVQPKGWLEWL 275

Query: 326 NSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGV 385
               + LL+Y EY+NRG GAN S RV WPGFH + +  +  +F+V  FINGTTWLP+T +
Sbjct: 276 -GVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVAKFTVENFINGTTWLPQTTI 335

Query: 386 PYRPDL 391
           P+R  L
Sbjct: 336 PFRSGL 336

[Close]

BLAST of Carg04883 vs. ExPASy TrEMBL
Match: A0A6J1D863 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.6e-94
Identity = 180/305 (59.02%), Postives = 213/305 (69.84%), Query Frame = 0


Query: 87  GDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNRS 146
           G++  +  AIAAAPN S SRF IHVKPG Y E++++ + KTFI L GDDASTT IV NRS
Sbjct: 39  GNFFRVNDAIAAAPNFSTSRFYIHVKPGTYNEHVEVPAQKTFIALIGDDASTTIIVDNRS 98

Query: 147 QATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVFL 206
             TGF TA SAT  V   +F+A S+TF NS  AGP  GQAVA+L  +   +TV+YKCVFL
Sbjct: 99  NGTGFSTATSATLTVSGENFMAQSLTFQNS--AGPDKGQAVAVL--DQAKHTVYYKCVFL 158

Query: 207 GYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNIK 266
           GYQDTLF      FFK+C+IYG+VDFIFGN L +FQDC IYARL +  +TVTAQSKDN+ 
Sbjct: 159 GYQDTLFAGAVPQFFKECDIYGSVDFIFGNGLAIFQDCNIYARLSDIHITVTAQSKDNVD 218

Query: 267 QLSGFSFQNCSVMVSPEL-GPVNETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWSN 326
             SGF FQ C+V VSPE+    N+  VFLGRPWR YS V F+ESFLD VV P GW EW  
Sbjct: 219 NHSGFIFQACNVTVSPEIVSSKNKVTVFLGRPWRTYSMVAFIESFLDDVVQPKGWLEWP- 278

Query: 327 SSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGVP 386
            + + LLFYAEY N G G+  S RV WPGFH      E   F+V  FI+GT WLPETG+P
Sbjct: 279 GTPVNLLFYAEYKNSGTGSETSNRVDWPGFHLFSYAAEVANFTVENFIDGTQWLPETGIP 338

Query: 387 YRPDL 391
           +R  L
Sbjct: 339 FRTGL 338

[Close]

BLAST of Carg04883 vs. ExPASy TrEMBL
Match: A0A6J1GIG0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 3.6e-94
Identity = 178/306 (58.17%), Postives = 222/306 (72.55%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           TG++  I  AI AAPN + +RF IH+KPG+Y E+I++   KTFI+L GD+ASTT IV NR
Sbjct: 36  TGNFVKISDAIVAAPNFTTTRFYIHIKPGIYNEHIEVPFQKTFISLIGDNASTTIIVDNR 95

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S+A GF T++SAT  V   +F+A  +TF NS  AGPK GQAVA+L  +   +T +YKCVF
Sbjct: 96  SKAAGFSTSNSATLTVNGANFMAKFLTFQNS--AGPKMGQAVAVL--DQAKHTAYYKCVF 155

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265
           LGYQDTL+      FFK+C+IYGTVDFIFGN LVVFQDC IYARL++   TVTAQSK N+
Sbjct: 156 LGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVVFQDCNIYARLVDIHTTVTAQSKANL 215

Query: 266 KQLSGFSFQNCSVMVSPELGPV-NETIVFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWS 325
            + SGF FQNC+V VSPE+    ++ +VFLGRPWR YS V F+ESFLD+VV P GW EW 
Sbjct: 216 DEQSGFVFQNCTVTVSPEIASSRDKVLVFLGRPWRAYSMVAFVESFLDNVVQPKGWLEWL 275

Query: 326 NSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGV 385
               + LL+Y EY+NRG GAN S RV WPGFH + +  +   F+V  FINGTTWLPET +
Sbjct: 276 -GVPVNLLYYGEYNNRGGGANTSQRVDWPGFHVLHSLTDVANFTVENFINGTTWLPETTI 335

Query: 386 PYRPDL 391
           P+R  L
Sbjct: 336 PFRAGL 336

[Close]

BLAST of Carg04883 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 1.1e-93
Identity = 172/306 (56.21%), Postives = 218/306 (71.24%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           +G++  +  AIAAAPN S +RF IHVKPG+Y E++++   KTFI L G+DASTT IV NR
Sbjct: 38  SGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKEHVEVPVQKTFIALIGEDASTTIIVDNR 97

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S  +GF T  +AT +V   +F+A  +TF NS  A  K GQA+A++  +   +T +YKCVF
Sbjct: 98  SNGSGFSTQTTATLIVKGQNFMAQFLTFENS--AEQKEGQAIAVM--DQAKHTAYYKCVF 157

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLNNTVTVTAQSKDNI 265
           L YQDTL+      FFK+C+IYG +DFIFGN LVVFQDC IYAR+    + VTAQSK+ +
Sbjct: 158 LSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQDCNIYARIPGTHIIVTAQSKNAV 217

Query: 266 KQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSEWS 325
           +  SGF FQNC+V VSPE+    + + +FLGRPWRDYS VIFMESFLD VV P GW EW 
Sbjct: 218 EDQSGFIFQNCNVTVSPEIASSKDNVTMFLGRPWRDYSMVIFMESFLDRVVQPKGWLEWP 277

Query: 326 NSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPETGV 385
               + LLFYAEY+NRGPGA+ S RV WPG+H + N  E T+F+V  FINGT WLPETG+
Sbjct: 278 -GRPVNLLFYAEYNNRGPGADTSQRVDWPGYHVLHNAMEVTKFTVENFINGTKWLPETGI 337

Query: 386 PYRPDL 391
           P+R  L
Sbjct: 338 PFRAGL 338

[Close]

BLAST of Carg04883 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 287.7 bits (735), Expect = 1.4e-77
Identity = 141/304 (46.38%), Postives = 202/304 (66.45%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           TG++TTI  A+ AAPN S++RFII++K G YFE +++   KT I   GD    T I  NR
Sbjct: 229 TGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANR 288

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S+  G+ T  + T  V    ++A  I+F NS  AGP   QAVA  + +  +++ FY+C F
Sbjct: 289 SRIDGWSTFQTPTVGVKGKGYIAKDISFVNS--AGPAKAQAVAFRSGS--DHSAFYRCEF 348

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARLLN--NTVTVTAQSKD 265
            GYQDTL+++    F+++C+IYGT+DFIFGNA VVFQ+  +YAR  N  + +  TAQS++
Sbjct: 349 DGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRN 408

Query: 266 NIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSE 325
              Q +G S  NC ++ +P+L PV E    +LGRPWR YS  + ++SF+D +++PAGW E
Sbjct: 409 QSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLE 468

Query: 326 WSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPET 385
                +L+ L+Y EY N GPGAN++ RV WPGF  IEN+ EAT+F+V  FI+G+TWL  T
Sbjct: 469 GKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGPFIDGSTWLNST 528

Query: 386 GVPY 387
           G+P+
Sbjct: 529 GIPF 528

[Close]

BLAST of Carg04883 vs. TAIR 10
Match: AT4G02320.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 283.5 bits (724), Expect = 2.6e-76
Identity = 141/308 (45.78%), Postives = 207/308 (67.21%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSRFIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIVGNR 145
           TG+YTTIG AI+AAPN+S++RF+I++K G YFE I+I   KT I   GD    T I  NR
Sbjct: 215 TGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIGRTVIKANR 274

Query: 146 SQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYKCVF 205
           S A G+    SAT  V  + F+A  ++F N   AGP+  QAVAL +S+++  + +Y+C F
Sbjct: 275 SYADGWTAFHSATVGVRGSGFIAKDLSFVNY--AGPEKHQAVALRSSSDL--SAYYRCSF 334

Query: 206 LGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYARL--LNNTVTVTAQSKD 265
             YQDT++++    F+++C+IYGTVDFIFG+A VVFQ+C +YAR    N  +  TAQ ++
Sbjct: 335 ESYQDTIYVHSHKQFYRECDIYGTVDFIFGDASVVFQNCSLYARRPNPNQKIIYTAQGRE 394

Query: 266 NIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAGWSE 325
           N ++ +G S  +  ++ +P+L PV      +LGRPW+ YS  + M+SF+D +V+PAGW +
Sbjct: 395 NSREPTGISIISSRILAAPDLIPVQANFKAYLGRPWQLYSRTVIMKSFIDDLVDPAGWLK 454

Query: 326 WSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWLPET 385
           W +  +L+ L+Y EY N GPG+N++ RV+WPGF  IE  +EA++FSV  FI+G  WL  T
Sbjct: 455 WKDDFALETLYYGEYMNEGPGSNMTNRVQWPGFKRIETVEEASQFSVGPFIDGNKWLNST 514

Query: 386 GVPYRPDL 391
            +P+  DL
Sbjct: 515 RIPFTLDL 518

[Close]

BLAST of Carg04883 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 281.2 bits (718), Expect = 1.3e-75
Identity = 140/311 (45.02%), Postives = 203/311 (65.27%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSR---FIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIV 145
           TG++T I AA+AAAPNN+D     F+I+V  G+Y EYI I  +K ++ + GD  + T + 
Sbjct: 272 TGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVT 331

Query: 146 GNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYK 205
           GNRS   G+ T +SATF V A +F+AV+ITF N+  AGP+  QAVAL   +  ++++FY 
Sbjct: 332 GNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNT--AGPEKHQAVAL--RSGADFSIFYS 391

Query: 206 CVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYAR--LLNNTVTVTAQ 265
           C F  YQDTL+ +    F+++C++YGTVDFIFGNA VVFQ+C +Y R  + N    +TAQ
Sbjct: 392 CSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQ 451

Query: 266 SKDNIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAG 325
            + +  Q +G S QNC++  + +L   N T+  +LGRPW++YS  ++M+S++D  V P G
Sbjct: 452 GRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVG 511

Query: 326 WSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWL 385
           W EW+   +L  L+YAEY+N GPG+N + RV WPG+H I N  +A  F+V        W+
Sbjct: 512 WREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVI-NSTDAANFTVTGLFIEADWI 571

Query: 386 PETGVPYRPDL 391
            +TGVPY   L
Sbjct: 572 WKTGVPYTSGL 577

[Close]

BLAST of Carg04883 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 270.8 bits (691), Expect = 1.7e-72
Identity = 131/307 (42.67%), Postives = 201/307 (65.47%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSR---FIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIV 145
           TG++TTI AAIAAAPN +D     F+I+V  G+Y EY+++  +K ++ + GD  + T I 
Sbjct: 253 TGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVIT 312

Query: 146 GNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYK 205
           GNRS   G+ T +SATF++   +F+ V+IT  N+  AGP  GQAVAL +  ++  +VFY 
Sbjct: 313 GNRSVVDGWTTFNSATFILSGPNFIGVNITIRNT--AGPTKGQAVALRSGGDL--SVFYS 372

Query: 206 CVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYAR--LLNNTVTVTAQ 265
           C F  YQDTL+ +    F+++C++YGTVDFIFGNA VV Q+C +Y R      +  VTAQ
Sbjct: 373 CSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQ 432

Query: 266 SKDNIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAG 325
            + +  Q +G +   C++  + +L   N T+  +LGRPW++YS  + M++++D  + P+G
Sbjct: 433 GRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSG 492

Query: 326 WSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWL 385
           W+ WS   +L  L+YAEY+N GPG++ + RV WPG+H I N  +A+ F+V  F+ G  W+
Sbjct: 493 WNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWI 552

Query: 386 PETGVPY 387
            +TGVP+
Sbjct: 553 GQTGVPF 554

[Close]

BLAST of Carg04883 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 270.8 bits (691), Expect = 1.7e-72
Identity = 133/307 (43.32%), Postives = 201/307 (65.47%), Query Frame = 0


Query: 86  TGDYTTIGAAIAAAPNNSDSR---FIIHVKPGMYFEYIKIESSKTFITLKGDDASTTTIV 145
           TG++TTI  A+ +APN +D     F+I+V  G+Y E + I  +K ++ + GD  + T + 
Sbjct: 266 TGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVT 325

Query: 146 GNRSQATGFGTADSATFVVGANHFLAVSITFSNSAGAGPKHGQAVALLNSNNVNYTVFYK 205
           GNR+   G+ T +SATF V + +F+AV++TF N+  AGP+  QAVA+ +S   + ++FY 
Sbjct: 326 GNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT--AGPEKHQAVAMRSS--ADLSIFYS 385

Query: 206 CVFLGYQDTLFINGKMLFFKDCEIYGTVDFIFGNALVVFQDCRIYAR--LLNNTVTVTAQ 265
           C F  YQDTL+ +    F+++C+IYGTVDFIFGNA VVFQDC +Y R  + N    +TAQ
Sbjct: 386 CSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAITAQ 445

Query: 266 SKDNIKQLSGFSFQNCSVMVSPELGPVNETI-VFLGRPWRDYSTVIFMESFLDSVVNPAG 325
            + +  Q +G S  NC++  + +L   N T+  +LGRPW++YS  +FM+S++D VV P G
Sbjct: 446 GRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVEPVG 505

Query: 326 WSEWSNSSSLKLLFYAEYDNRGPGANLSGRVKWPGFHAIENRKEATRFSVREFINGTTWL 385
           W EW+   +L  L+YAEY+N G G++ + RV WPG+H I N  +A  F+V  F+ G  W+
Sbjct: 506 WREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDANNFTVENFLLGDGWM 565

Query: 386 PETGVPY 387
            ++GVPY
Sbjct: 566 VQSGVPY 567

The following BLAST results are available for this feature:

BLAST of Carg04883 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019877.1	5.0e-223	100.00	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584282.1	3.2e-169	97.37	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584281.1	5.4e-169	97.37	putative pectinesterase/pectinesterase inhibitor 39, partial [Cucurbita argyrosp...	[more]
KAG6584284.1	4.8e-109	71.15	putative pectinesterase/pectinesterase inhibitor 42, partial [Cucurbita argyrosp...	[more]
XP_022148123.1	4.2e-97	58.50	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]

BLAST of Carg04883 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	1.9e-76	46.38	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O81301	3.6e-75	45.78	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	1.8e-74	45.02	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]
O22256	2.4e-71	42.67	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
Q8RXK7	2.4e-71	43.32	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg04883 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D381	2.1e-97	58.50	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016876 PE=4 SV=1	[more]
A0A6J1KPG6	1.1e-95	59.15	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496427 PE=4 SV=1	[more]
A0A6J1D863	1.6e-94	59.02	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018522 PE=4 SV=1	[more]
A0A6J1GIG0	3.6e-94	58.17	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454459 PE=4 SV=1	[more]
A0A6J1DAG5	1.1e-93	56.21	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]

BLAST of Carg04883 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	1.4e-77	46.38	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02320.1	2.6e-76	45.78	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	1.3e-75	45.02	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	1.7e-72	42.67	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	1.7e-72	43.32	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 86..375 e-value: 3.9E-100 score: 334.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 81..390 e-value: 2.4E-105 score: 354.4
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 52..387
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 52..387
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 226..235
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 86..388

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04883-RA	Carg04883-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity