Carg04750 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg04750 vs. NCBI nr
Match: XP_022924022.1 (diacylglycerol kinase 1-like [Cucurbita moschata] >XP_022924023.1 diacylglycerol kinase 1-like [Cucurbita moschata] >KAG7020006.1 Diacylglycerol kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1482.6 bits (3837), Expect = 0.0e+00
Identity = 728/728 (100.00%), Postives = 728/728 (100.00%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300
           ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360
           ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP
Sbjct: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360

Query: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420
           LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD
Sbjct: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420

Query: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480
           GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN
Sbjct: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480

Query: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540
           AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN
Sbjct: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540

Query: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600
           KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE
Sbjct: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600

Query: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660
           DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID
Sbjct: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660

Query: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720
           GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL
Sbjct: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720

Query: 721 QEIAKRLT 729
           QEIAKRLT
Sbjct: 721 QEIAKRLT 728

BLAST of Carg04750 vs. NCBI nr
Match: XP_023519698.1 (diacylglycerol kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1473.4 bits (3813), Expect = 0.0e+00
Identity = 723/728 (99.31%), Postives = 725/728 (99.59%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300
           ITHGANEIASSVRASIRSQSKKNKHSR+PSIHASKSGSIRDMSTESTADSHHTVNGYNGT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRRPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360
           ERNINGSRTSELQHQNGDI+DKNISN SFKKNSSFNQKD THVGMNLRYEVIELPPDSRP
Sbjct: 301 ERNINGSRTSELQHQNGDINDKNISNPSFKKNSSFNQKDETHVGMNLRYEVIELPPDSRP 360

Query: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420
           LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD
Sbjct: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420

Query: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480
           GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN
Sbjct: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480

Query: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540
           AAV VLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN
Sbjct: 481 AAVAVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540

Query: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600
           KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE
Sbjct: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600

Query: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660
           DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID
Sbjct: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660

Query: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720
           GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL
Sbjct: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720

Query: 721 QEIAKRLT 729
           QEIAKRLT
Sbjct: 721 QEIAKRLT 728

BLAST of Carg04750 vs. NCBI nr
Match: XP_023000647.1 (diacylglycerol kinase 1-like [Cucurbita maxima] >XP_023000648.1 diacylglycerol kinase 1-like [Cucurbita maxima])

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 722/728 (99.18%), Postives = 725/728 (99.59%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLY+KESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYIKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300
           ITHGANEIAS+VRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT
Sbjct: 241 ITHGANEIASTVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360
           ERNINGSRTSELQHQNGDI DKNISN SFKK+SSFNQKD THVGMNLRYEVIELPPDSRP
Sbjct: 301 ERNINGSRTSELQHQNGDI-DKNISNPSFKKSSSFNQKDETHVGMNLRYEVIELPPDSRP 360

Query: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420
           LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD
Sbjct: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420

Query: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480
           GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN
Sbjct: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480

Query: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540
           AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN
Sbjct: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540

Query: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600
           KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE
Sbjct: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600

Query: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660
           DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID
Sbjct: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660

Query: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720
           GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL
Sbjct: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720

Query: 721 QEIAKRLT 729
           QEIAKRLT
Sbjct: 721 QEIAKRLT 727

BLAST of Carg04750 vs. NCBI nr
Match: XP_008448453.1 (PREDICTED: diacylglycerol kinase 1 isoform X2 [Cucumis melo] >TYJ98925.1 diacylglycerol kinase 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 680/730 (93.15%), Postives = 710/730 (97.26%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDE+WEIGLLFPSWNSKNPTDRLFVISC SAA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT +VPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF++VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSI-HASKSGSIRDMSTESTADSHHTVNGYNG 300
           ITHGANEIASSVRASIRSQSKK+KHSRKPS+ H   SG++RDMSTESTADSH TVNGY+G
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDS 360
           TERN NGSRTSE +HQNGDI DK+ISN+S KK+SS N KD TH+ GMNLRYEVIE+P DS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 660
           NEDETFD+FDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQ++PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEIAKRLT 729
           LLQE+AKRLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of Carg04750 vs. NCBI nr
Match: XP_011650112.1 (diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744670.1 diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744672.1 diacylglycerol kinase 1 isoform X1 [Cucumis sativus] >XP_031744674.1 diacylglycerol kinase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 681/730 (93.29%), Postives = 709/730 (97.12%), Query Frame = 0

Query: 2   DEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIARS 61
           DEEWEIGLLFPSWNSKNPTDRLFVISC SAAIIGILTIAFTAFQWRRNINLSWM+AIARS
Sbjct: 3   DEEWEIGLLFPSWNSKNPTDRLFVISCFSAAIIGILTIAFTAFQWRRNINLSWMRAIARS 62

Query: 62  K-RNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 121
           K RNPKKT +VPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 63  KRRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 122

Query: 122 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 181
           GVAAHLSCSSNAQKDCKCVSMIGF++VMHQWAVRWTEITDQ DETSFCSYCEEPCSGSFL
Sbjct: 123 GVAAHLSCSSNAQKDCKCVSMIGFDHVMHQWAVRWTEITDQSDETSFCSYCEEPCSGSFL 182

Query: 182 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 241
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 183 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNRI-SGGFLSS 242

Query: 242 ITHGANEIASSVRASIRSQSKKNKHSRKPSI-HASKSGSIRDMSTESTADSHHTVNGYNG 301
           ITHGANEIASSVRASIRSQSKK+KHSRKPSI H   SG++RDMSTESTADSHH VNGY+G
Sbjct: 243 ITHGANEIASSVRASIRSQSKKSKHSRKPSIHHTGSSGNLRDMSTESTADSHHRVNGYHG 302

Query: 302 TERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDS 361
           TERN NGSRTSE +HQNGDI+DK+ISN+S KKNSS N KD TH+ GMNLRYEVIE+P D+
Sbjct: 303 TERNCNGSRTSEGRHQNGDINDKSISNTSLKKNSSLNHKDETHILGMNLRYEVIEMPSDA 362

Query: 362 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCG 421
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCG
Sbjct: 363 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 422

Query: 422 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHV 481
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HV
Sbjct: 423 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 482

Query: 482 ENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 541
           ENAAVT+LDRWKVA+VDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 483 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 542

Query: 542 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 601
           MNKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 543 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 602

Query: 602 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 661
           NEDETFD+FD+QSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQ
Sbjct: 603 NEDETFDNFDAQSMHDKLLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 662

Query: 662 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 721
           IDGEPWFQ++PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 663 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 722

Query: 722 LLQEIAKRLT 729
           LLQE+AKRLT
Sbjct: 723 LLQEMAKRLT 731

BLAST of Carg04750 vs. ExPASy Swiss-Prot
Match: Q39017 (Diacylglycerol kinase 1 OS=Arabidopsis thaliana OX=3702 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 531/736 (72.15%), Postives = 618/736 (83.97%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTD----RLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMK 60
           MD++ E+G+ FPSW SKNP D    R  + SC  AA++GILTIA+TAFQWRRNINLSW K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHR 120
           AIARSK+NPK  +KVPVA H W L+ ++R KNL+CCVCLK +SPSQ +   VAS+SF HR
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CNICGVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCS 180
           C ICG AAH +CSS+A KDCKCVSM+GFE+V+HQWAVRWTE  DQ D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGG 240
            SFLGGSPIWCCLWCQR+VHVDCHS+M NETGD+CDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNG 300
           FLSSITHGANE+AS+  ASIR QSKK K + + S     SGS  D STESTAD+  TVNG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YNGTERN----INGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVI 360
            +    N    +NG  ++     NG ++ K     S K+  SF QK+   +   L+YE+ 
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKK----PSVKRTGSFGQKEYHALRSKLKYELA 360

Query: 361 ELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHF 420
           +LP D+RPLLVFINKKSGA+RGDSL+QRL++ LNPVQVFELSS QGPE GL+LFRKVPHF
Sbjct: 361 DLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHF 420

Query: 421 KVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLC 480
           +VLVCGGDGT GWVL+ I+KQNF+SPP VAILPAGTGNDL+RVLNWGGGLGSVERQGGL 
Sbjct: 421 RVLVCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLS 480

Query: 481 TVLRHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPE 540
           TVL+++E+AAVTVLDRWKV+I++QQGKQL+ P++MNNY+G+GCDAKVAL+IHNLREENPE
Sbjct: 481 TVLQNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPE 540

Query: 541 KFYNQFMNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMG 600
           +FY+QFMNKVLYAREGA+SIMDRT+ D PWQVRVEVDGV++EVPEDAEG+LVANIGSYMG
Sbjct: 541 RFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMG 600

Query: 601 GVDLWHNEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLC 660
           GVDLW NEDET+++FD QSMHDKI+EVVSISGTWHLGKLQVGLSRARRLAQG +++IQLC
Sbjct: 601 GVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLC 660

Query: 661 AALPVQIDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVIN 720
           A LPVQIDGEPW Q  PCTL ISHHG+AFMLKRA EEPLGHAAAIITDVLE+AE+N VIN
Sbjct: 661 APLPVQIDGEPWNQQ-PCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVIN 720

Query: 721 ASQKRVLLQEIAKRLT 729
           ASQKR LLQE+A RLT
Sbjct: 721 ASQKRTLLQEMALRLT 728

BLAST of Carg04750 vs. ExPASy Swiss-Prot
Match: Q9FFN7 (Diacylglycerol kinase 2 OS=Arabidopsis thaliana OX=3702 GN=DGK2 PE=1 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 1.0e-200
Identity = 360/730 (49.32%), Postives = 486/730 (66.58%), Query Frame = 0

Query: 6   EIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIARSKRNP 65
           E+G     W   +  D  F+   L    +G+L + +T  +W++  +L+W+KA AR K+  
Sbjct: 3   EVGFSLIQWLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKV 62

Query: 66  KKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNICGVAAH 125
            K  +VP++ H W  +     +  +CCVCL  + P Q +    +    +HRC +CGVAAH
Sbjct: 63  WKRLRVPLSHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAH 122

Query: 126 LSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPI 185
             CSS+A KDCKCV+  G ++V H W+ RW  + D  D T+FC YC+EPC   F+  SP+
Sbjct: 123 FYCSSSAAKDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPM 182

Query: 186 WCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSSITHGA 245
           W CLWCQR++HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    
Sbjct: 183 WHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---K 242

Query: 246 NEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNG-TERNI 305
           NE+     ASIR   ++ +H  K     S +G + +   +S +D   TV   NG   + +
Sbjct: 243 NEL-----ASIRGHVRRKRHRGKNGNGQSLNGKLLE---DSVSDPVKTV--VNGLVVKKL 302

Query: 306 NGSRTSELQHQNGDIDD-KNISN--SSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRPL 365
              R+ +   Q  D+ + K + N     K+N S        +    ++ +++LPPD+RPL
Sbjct: 303 RRDRSIDCLKQVSDMPNAKGLQNGIGGHKRNKS------AALNFMKKFSLVDLPPDARPL 362

Query: 366 LVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDG 425
           LVFIN KSG + G  L +RLNMLLNPVQVFEL S QGP++GL L  KV +F+VLVCGGDG
Sbjct: 363 LVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDG 422

Query: 426 TVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVENA 485
           TV WVL+ I+K+NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L+ +++A
Sbjct: 423 TVAWVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHA 482

Query: 486 AVTVLDRWKVAIVDQQGKQL---KSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 545
           AVT+LDRW V IV++  ++    +  +FM NYLGIGCDAKVA + H +R+E PEKF +QF
Sbjct: 483 AVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQF 542

Query: 546 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 605
           +NK+ YA+EGA+ IMDR  AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW 
Sbjct: 543 VNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQ 602

Query: 606 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 665
           N+ E  D+F  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQG+ IRI + +  PVQ
Sbjct: 603 NDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQ 662

Query: 666 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 725
           IDGEP+ Q   C L I+HHG+ FML+RA +EP GHAAAI+ +VL  AE   VINASQK+V
Sbjct: 663 IDGEPFIQQPGC-LEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKV 712

Query: 726 LLQEIAKRLT 729
           LLQ++A  L+
Sbjct: 723 LLQQMALHLS 712

BLAST of Carg04750 vs. ExPASy Swiss-Prot
Match: P49621 (Diacylglycerol kinase beta OS=Rattus norvegicus OX=10116 GN=Dgkb PE=1 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.1e-72
Identity = 156/384 (40.62%), Postives = 210/384 (54.69%), Query Frame = 0

Query: 326 NSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLL 385
           N    KN       VT  G  L+   I   P + PLLVF+N KSG ++G+ + ++   LL
Sbjct: 404 NKVTDKNKMQRANSVTMDGQGLQITPI---PGTHPLLVFVNPKSGGKQGERIYRKFQYLL 463

Query: 386 NPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILP 445
           NP QV+ L S  GP  GL+ FR VP F+VL CGGDGTVGW+L+CI+K N V  PPVAILP
Sbjct: 464 NPRQVYSL-SGNGPMPGLHFFRDVPDFRVLACGGDGTVGWILDCIEKANVVKHPPVAILP 523

Query: 446 AGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQLKSP- 505
            GTGNDLAR L WGGG         L  +L+ +E++   +LDRWK  +      +   P 
Sbjct: 524 LGTGNDLARCLRWGGGY----EGENLMKILKDIESSTEIMLDRWKFEVTPNDKDEKGDPV 583

Query: 506 --QFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTYADIPW 565
               +NNY  IG DA +A   H +RE++PEKF ++  NK  Y   G       T   +  
Sbjct: 584 PYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHE 643

Query: 566 QVRVEVDGVEVE-VPEDAEGVLVANIGSYMGGVDLW--------HNEDETFDS------- 625
            V +E DGV+++ +    +G+ + NI S  GG +LW        H   E   S       
Sbjct: 644 SVEIECDGVQIDLINISLQGIAILNIPSMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLT 703

Query: 626 ------FDSQSMHDKILEVVSISGTWHLGKLQVGL-SRARRLAQGRSIRIQLCAALPVQI 684
                 F SQ + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+   +LP+QI
Sbjct: 704 DAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQI 763

BLAST of Carg04750 vs. ExPASy Swiss-Prot
Match: Q9Y6T7 (Diacylglycerol kinase beta OS=Homo sapiens OX=9606 GN=DGKB PE=1 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 1.2e-71
Identity = 156/384 (40.62%), Postives = 208/384 (54.17%), Query Frame = 0

Query: 326 NSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLL 385
           N    KN       VT  G  L+   +   P + PLLVF+N KSG ++G+ + ++   LL
Sbjct: 407 NKVIDKNKMQRANSVTVDGQGLQVTPV---PGTHPLLVFVNPKSGGKQGERIYRKFQYLL 466

Query: 386 NPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILP 445
           NP QV+ L S  GP  GL  FR VP F+VL CGGDGTVGWVL+CI+K N    PPVAILP
Sbjct: 467 NPRQVYSL-SGNGPMPGLNFFRDVPDFRVLACGGDGTVGWVLDCIEKANVGKHPPVAILP 526

Query: 446 AGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQLKSP- 505
            GTGNDLAR L WGGG         L  +L+ +EN+   +LDRWK  ++     +   P 
Sbjct: 527 LGTGNDLARCLRWGGGY----EGENLMKILKDIENSTEIMLDRWKFEVIPNDKDEKGDPV 586

Query: 506 --QFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTYADIPW 565
               +NNY  IG DA +A   H +RE++PEKF ++  NK  Y   G       T   +  
Sbjct: 587 PYSIINNYFSIGVDASIAHRFHIMREKHPEKFNSRMKNKFWYFEFGTSETFSATCKKLHE 646

Query: 566 QVRVEVDGVEVE-VPEDAEGVLVANIGSYMGGVDLW--------HNEDETFDS------- 625
            V +E DGV+++ +    EG+ + NI S  GG +LW        H   E   S       
Sbjct: 647 SVEIECDGVQIDLINISLEGIAILNIPSMHGGSNLWGESKKRRSHRRIEKKGSDKRTTVT 706

Query: 626 ------FDSQSMHDKILEVVSISGTWHLGKLQVGL-SRARRLAQGRSIRIQLCAALPVQI 684
                 F SQ + D++LEVV + G   +G++  GL S  RRLAQ   + I+   +LP+QI
Sbjct: 707 DAKELKFASQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSCVVIRTSKSLPMQI 766

BLAST of Carg04750 vs. ExPASy Swiss-Prot
Match: Q6NS52 (Diacylglycerol kinase beta OS=Mus musculus OX=10090 GN=Dgkb PE=1 SV=2)

HSP 1 Score: 272.3 bits (695), Expect = 1.6e-71
Identity = 197/597 (33.00%), Postives = 269/597 (45.06%), Query Frame = 0

Query: 117 CNICGVAAHLSCSSNAQKDCKCVSMIGFEN--VMHQWAVRWTEITDQPDETSFCSYCEE- 176
           C+ C    H  C + A   C    +   +N  VMH +   W E     +  + C  C + 
Sbjct: 274 CSFCKYTVHERCVARAPPSCIKTYVKSKKNTDVMHHY---WVE----GNCPTKCDKCHKT 333

Query: 177 -PCSGSFLGGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRT 236
             C     G      C+WCQ  +H  C S +  E    CD G  +  IL P        T
Sbjct: 334 VKCYQGLTG----LHCVWCQTTLHNKCASHLKPE----CDCGPLKDHILPP-------TT 393

Query: 237 SSGGFLSSITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHH 296
                L+  + GA     SV    +S +KK K S +                        
Sbjct: 394 ICPVVLTMPSAGA-----SVPEERQSTAKKEKSSSQQ----------------------- 453

Query: 297 TVNGYNGTERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVI 356
                                            N +  KN       VT  G  L+   +
Sbjct: 454 --------------------------------PNKATDKNKMQRANSVTMDGQGLQITPV 513

Query: 357 ELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHF 416
              P + PLLVF+N KSG ++G+ + ++   LLNP QV+ L S  GP  GL+ FR VP F
Sbjct: 514 ---PGTHPLLVFVNPKSGGKQGERIYRKFQYLLNPRQVYSL-SGNGPMPGLHFFRDVPDF 573

Query: 417 KVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLC 476
           +VL CGGDGTVGW+L+CI+K N V  PPVAILP GTGNDLAR L WGGG         L 
Sbjct: 574 RVLACGGDGTVGWILDCIEKANVVKHPPVAILPLGTGNDLARCLRWGGGY----EGENLM 633

Query: 477 TVLRHVENAAVTVLDRWKVAIVDQQGKQLKSP---QFMNNYLGIGCDAKVALDIHNLREE 536
            +L+ +E++   +LDRWK  +      +   P     +NNY  IG DA +A   H +RE+
Sbjct: 634 KILKDIESSTEIMLDRWKFEVTPNDKDEKGDPVPYSIINNYFSIGVDASIAHRFHIMREK 693

Query: 537 NPEKFYNQFMNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVE-VPEDAEGVLVANIG 596
           +PEKF ++  NK  Y   G       T   +   V +E DGV+++ +    EG+ + NI 
Sbjct: 694 HPEKFNSRMKNKFWYFEFGTSETFSATCKKLHESVEIECDGVQIDLINISLEGIAILNIP 753

Query: 597 SYMGGVDLW--------HNEDETFDS-------------FDSQSMHDKILEVVSISGTWH 656
           S  GG +LW        H   E   S             F SQ + D++LEVV + G   
Sbjct: 754 SMHGGSNLWGESKKKRSHRRIEKKGSDKRPTLTDAKELKFASQDLSDQLLEVVGLEGAME 779

Query: 657 LGKLQVGL-SRARRLAQGRSIRIQLCAALPVQIDGEPWFQDIPCTLVISHHGEAFML 684
           +G++  GL S  RRLAQ  S+ I+   +LP+QIDGEPW Q  PCT+ I+H  +A ML
Sbjct: 814 MGQIYTGLKSAGRRLAQCSSVVIRTSKSLPMQIDGEPWMQ-TPCTIKITHKNQAPML 779

BLAST of Carg04750 vs. ExPASy TrEMBL
Match: A0A6J1E7Q5 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111431571 PE=3 SV=1)

HSP 1 Score: 1482.6 bits (3837), Expect = 0.0e+00
Identity = 728/728 (100.00%), Postives = 728/728 (100.00%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300
           ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360
           ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP
Sbjct: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360

Query: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420
           LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD
Sbjct: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420

Query: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480
           GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN
Sbjct: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480

Query: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540
           AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN
Sbjct: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540

Query: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600
           KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE
Sbjct: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600

Query: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660
           DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID
Sbjct: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660

Query: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720
           GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL
Sbjct: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720

Query: 721 QEIAKRLT 729
           QEIAKRLT
Sbjct: 721 QEIAKRLT 728

BLAST of Carg04750 vs. ExPASy TrEMBL
Match: A0A6J1KN74 (Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111495021 PE=3 SV=1)

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 722/728 (99.18%), Postives = 725/728 (99.59%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLY+KESNRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYIKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300
           ITHGANEIAS+VRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT
Sbjct: 241 ITHGANEIASTVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRP 360
           ERNINGSRTSELQHQNGDI DKNISN SFKK+SSFNQKD THVGMNLRYEVIELPPDSRP
Sbjct: 301 ERNINGSRTSELQHQNGDI-DKNISNPSFKKSSSFNQKDETHVGMNLRYEVIELPPDSRP 360

Query: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420
           LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD
Sbjct: 361 LLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGD 420

Query: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480
           GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN
Sbjct: 421 GTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVEN 480

Query: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540
           AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN
Sbjct: 481 AAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 540

Query: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600
           KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE
Sbjct: 541 KVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWHNE 600

Query: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660
           DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID
Sbjct: 601 DETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQID 660

Query: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720
           GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL
Sbjct: 661 GEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRVLL 720

Query: 721 QEIAKRLT 729
           QEIAKRLT
Sbjct: 721 QEIAKRLT 727

BLAST of Carg04750 vs. ExPASy TrEMBL
Match: A0A5D3BKN2 (Diacylglycerol kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001240 PE=3 SV=1)

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 680/730 (93.15%), Postives = 710/730 (97.26%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDE+WEIGLLFPSWNSKNPTDRLFVISC SAA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT +VPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF++VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSI-HASKSGSIRDMSTESTADSHHTVNGYNG 300
           ITHGANEIASSVRASIRSQSKK+KHSRKPS+ H   SG++RDMSTESTADSH TVNGY+G
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDS 360
           TERN NGSRTSE +HQNGDI DK+ISN+S KK+SS N KD TH+ GMNLRYEVIE+P DS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 660
           NEDETFD+FDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQ++PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEIAKRLT 729
           LLQE+AKRLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of Carg04750 vs. ExPASy TrEMBL
Match: A0A1S3BKC1 (Diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103490623 PE=3 SV=1)

HSP 1 Score: 1394.8 bits (3609), Expect = 0.0e+00
Identity = 680/730 (93.15%), Postives = 710/730 (97.26%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDE+WEIGLLFPSWNSKNPTDRLFVISC SAA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT +VPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF++VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSI-HASKSGSIRDMSTESTADSHHTVNGYNG 300
           ITHGANEIASSVRASIRSQSKK+KHSRKPS+ H   SG++RDMSTESTADSH TVNGY+G
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDS 360
           TERN NGSRTSE +HQNGDI DK+ISN+S KK+SS N KD TH+ GMNLRYEVIE+P DS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 660
           NEDETFD+FDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQ++PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEIAKRLT 729
           LLQE+AKRLT
Sbjct: 721 LLQEMAKRLT 729

BLAST of Carg04750 vs. ExPASy TrEMBL
Match: A0A1S4DTP2 (Diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103490623 PE=3 SV=1)

HSP 1 Score: 1389.8 bits (3596), Expect = 0.0e+00
Identity = 677/727 (93.12%), Postives = 707/727 (97.25%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60
           MDE+WEIGLLFPSWNSKNPTDRLFVISC SAA+IGILTIAFTAFQWRRNINLSWMKAIAR
Sbjct: 1   MDEDWEIGLLFPSWNSKNPTDRLFVISCFSAAVIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120
           SKRNPKKT +VPVAAHDWILESVSRGKNLSCCVCLK+VSPSQTLGPMVASDSFIHRCNIC
Sbjct: 61  SKRNPKKTQRVPVAAHDWILESVSRGKNLSCCVCLKYVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180
           GVAAHLSCSSNAQKDCKC+SMIGF++VMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCISMIGFDHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKESNR  SGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESNR-MSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSI-HASKSGSIRDMSTESTADSHHTVNGYNG 300
           ITHGANEIASSVRASIRSQSKK+KHSRKPS+ H   SG++RDMSTESTADSH TVNGY+G
Sbjct: 241 ITHGANEIASSVRASIRSQSKKSKHSRKPSVHHTGSSGNLRDMSTESTADSHLTVNGYHG 300

Query: 301 TERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHV-GMNLRYEVIELPPDS 360
           TERN NGSRTSE +HQNGDI DK+ISN+S KK+SS N KD TH+ GMNLRYEVIE+P DS
Sbjct: 301 TERNCNGSRTSEGRHQNGDIADKSISNTSLKKSSSLNHKDETHILGMNLRYEVIEMPSDS 360

Query: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCG 420
           RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS QGPESGLYLFRKVPHFKVLVCG
Sbjct: 361 RPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSTQGPESGLYLFRKVPHFKVLVCG 420

Query: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHV 480
           GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVL HV
Sbjct: 421 GDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHV 480

Query: 481 ENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540
           ENAAVT+LDRWKVA+VDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF
Sbjct: 481 ENAAVTLLDRWKVAMVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 540

Query: 541 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600
           MNKVLYAREGAKSIMDRT+ADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH
Sbjct: 541 MNKVLYAREGAKSIMDRTFADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 600

Query: 601 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 660
           NEDETFD+FDSQSMHDK+LEVVSISGTWHLGKLQVGLSRARRLAQG+SIRIQLCAALPVQ
Sbjct: 601 NEDETFDNFDSQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQ 660

Query: 661 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720
           IDGEPWFQ++PCTLVISHHG+AFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV
Sbjct: 661 IDGEPWFQEVPCTLVISHHGQAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 720

Query: 721 LLQEIAK 726
           LLQE+AK
Sbjct: 721 LLQEMAK 726

BLAST of Carg04750 vs. TAIR 10
Match: AT5G07920.1 (diacylglycerol kinase1 )

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 531/736 (72.15%), Postives = 618/736 (83.97%), Query Frame = 0

Query: 1   MDEEWEIGLLFPSWNSKNPTD----RLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMK 60
           MD++ E+G+ FPSW SKNP D    R  + SC  AA++GILTIA+TAFQWRRNINLSW K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHR 120
           AIARSK+NPK  +KVPVA H W L+ ++R KNL+CCVCLK +SPSQ +   VAS+SF HR
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CNICGVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCS 180
           C ICG AAH +CSS+A KDCKCVSM+GFE+V+HQWAVRWTE  DQ D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGG 240
            SFLGGSPIWCCLWCQR+VHVDCHS+M NETGD+CDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNG 300
           FLSSITHGANE+AS+  ASIR QSKK K + + S     SGS  D STESTAD+  TVNG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YNGTERN----INGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTHVGMNLRYEVI 360
            +    N    +NG  ++     NG ++ K     S K+  SF QK+   +   L+YE+ 
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKK----PSVKRTGSFGQKEYHALRSKLKYELA 360

Query: 361 ELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHF 420
           +LP D+RPLLVFINKKSGA+RGDSL+QRL++ LNPVQVFELSS QGPE GL+LFRKVPHF
Sbjct: 361 DLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPVQVFELSSVQGPEVGLFLFRKVPHF 420

Query: 421 KVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLC 480
           +VLVCGGDGT GWVL+ I+KQNF+SPP VAILPAGTGNDL+RVLNWGGGLGSVERQGGL 
Sbjct: 421 RVLVCGGDGTAGWVLDAIEKQNFISPPAVAILPAGTGNDLSRVLNWGGGLGSVERQGGLS 480

Query: 481 TVLRHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMNNYLGIGCDAKVALDIHNLREENPE 540
           TVL+++E+AAVTVLDRWKV+I++QQGKQL+ P++MNNY+G+GCDAKVAL+IHNLREENPE
Sbjct: 481 TVLQNIEHAAVTVLDRWKVSILNQQGKQLQPPKYMNNYIGVGCDAKVALEIHNLREENPE 540

Query: 541 KFYNQFMNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMG 600
           +FY+QFMNKVLYAREGA+SIMDRT+ D PWQVRVEVDGV++EVPEDAEG+LVANIGSYMG
Sbjct: 541 RFYSQFMNKVLYAREGARSIMDRTFEDFPWQVRVEVDGVDIEVPEDAEGILVANIGSYMG 600

Query: 601 GVDLWHNEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLC 660
           GVDLW NEDET+++FD QSMHDKI+EVVSISGTWHLGKLQVGLSRARRLAQG +++IQLC
Sbjct: 601 GVDLWQNEDETYENFDPQSMHDKIVEVVSISGTWHLGKLQVGLSRARRLAQGSAVKIQLC 660

Query: 661 AALPVQIDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVIN 720
           A LPVQIDGEPW Q  PCTL ISHHG+AFMLKRA EEPLGHAAAIITDVLE+AE+N VIN
Sbjct: 661 APLPVQIDGEPWNQQ-PCTLTISHHGQAFMLKRAAEEPLGHAAAIITDVLENAETNQVIN 720

Query: 721 ASQKRVLLQEIAKRLT 729
           ASQKR LLQE+A RLT
Sbjct: 721 ASQKRTLLQEMALRLT 728

BLAST of Carg04750 vs. TAIR 10
Match: AT5G63770.1 (diacylglycerol kinase 2 )

HSP 1 Score: 701.4 bits (1809), Expect = 7.4e-202
Identity = 360/730 (49.32%), Postives = 486/730 (66.58%), Query Frame = 0

Query: 6   EIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIARSKRNP 65
           E+G     W   +  D  F+   L    +G+L + +T  +W++  +L+W+KA AR K+  
Sbjct: 3   EVGFSLIQWLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKV 62

Query: 66  KKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNICGVAAH 125
            K  +VP++ H W  +     +  +CCVCL  + P Q +    +    +HRC +CGVAAH
Sbjct: 63  WKRLRVPLSHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAH 122

Query: 126 LSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFLGGSPI 185
             CSS+A KDCKCV+  G ++V H W+ RW  + D  D T+FC YC+EPC   F+  SP+
Sbjct: 123 FYCSSSAAKDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPM 182

Query: 186 WCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSSITHGA 245
           W CLWCQR++HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    
Sbjct: 183 WHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---K 242

Query: 246 NEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNG-TERNI 305
           NE+     ASIR   ++ +H  K     S +G + +   +S +D   TV   NG   + +
Sbjct: 243 NEL-----ASIRGHVRRKRHRGKNGNGQSLNGKLLE---DSVSDPVKTV--VNGLVVKKL 302

Query: 306 NGSRTSELQHQNGDIDD-KNISN--SSFKKNSSFNQKDVTHVGMNLRYEVIELPPDSRPL 365
              R+ +   Q  D+ + K + N     K+N S        +    ++ +++LPPD+RPL
Sbjct: 303 RRDRSIDCLKQVSDMPNAKGLQNGIGGHKRNKS------AALNFMKKFSLVDLPPDARPL 362

Query: 366 LVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPESGLYLFRKVPHFKVLVCGGDG 425
           LVFIN KSG + G  L +RLNMLLNPVQVFEL S QGP++GL L  KV +F+VLVCGGDG
Sbjct: 363 LVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDG 422

Query: 426 TVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLRHVENA 485
           TV WVL+ I+K+NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L+ +++A
Sbjct: 423 TVAWVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHA 482

Query: 486 AVTVLDRWKVAIVDQQGKQL---KSPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQF 545
           AVT+LDRW V IV++  ++    +  +FM NYLGIGCDAKVA + H +R+E PEKF +QF
Sbjct: 483 AVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQF 542

Query: 546 MNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVEVEVPEDAEGVLVANIGSYMGGVDLWH 605
           +NK+ YA+EGA+ IMDR  AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW 
Sbjct: 543 VNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQ 602

Query: 606 NEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCAALPVQ 665
           N+ E  D+F  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQG+ IRI + +  PVQ
Sbjct: 603 NDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQ 662

Query: 666 IDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLGHAAAIITDVLESAESNNVINASQKRV 725
           IDGEP+ Q   C L I+HHG+ FML+RA +EP GHAAAI+ +VL  AE   VINASQK+V
Sbjct: 663 IDGEPFIQQPGC-LEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKV 712

Query: 726 LLQEIAKRLT 729
           LLQ++A  L+
Sbjct: 723 LLQQMALHLS 712

BLAST of Carg04750 vs. TAIR 10
Match: AT5G63770.2 (diacylglycerol kinase 2 )

HSP 1 Score: 582.0 bits (1499), Expect = 6.6e-166
Identity = 306/576 (53.12%), Postives = 401/576 (69.62%), Query Frame = 0

Query: 160 DQPDETSFCSYCEEPCSGSFLGGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRL 219
           D  D T+FC YC+EPC   F+  SP+W CLWCQR++HV CH  M  E+GD CDLGS RR+
Sbjct: 3   DNADMTAFCFYCDEPCGIPFIEASPMWHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRV 62

Query: 220 ILSPLYVKESNRTSSGGFLSSITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSI 279
           ILSP++VK +      G L++I    NE+     ASIR   ++ +H  K     S +G +
Sbjct: 63  ILSPVHVKLNEANGVDGVLTTI---KNEL-----ASIRGHVRRKRHRGKNGNGQSLNGKL 122

Query: 280 RDMSTESTADSHHTVNGYNG-TERNINGSRTSELQHQNGDIDD-KNISN--SSFKKNSSF 339
            +   +S +D   TV   NG   + +   R+ +   Q  D+ + K + N     K+N S 
Sbjct: 123 LE---DSVSDPVKTV--VNGLVVKKLRRDRSIDCLKQVSDMPNAKGLQNGIGGHKRNKS- 182

Query: 340 NQKDVTHVGMNLRYEVIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSS 399
                  +    ++ +++LPPD+RPLLVFIN KSG + G  L +RLNMLLNPVQVFEL S
Sbjct: 183 -----AALNFMKKFSLVDLPPDARPLLVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGS 242

Query: 400 AQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGTGNDLARV 459
            QGP++GL L  KV +F+VLVCGGDGTV WVL+ I+K+NF SPPPVAILP GTGNDL+RV
Sbjct: 243 CQGPDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFESPPPVAILPLGTGNDLSRV 302

Query: 460 LNWGGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQL---KSPQFMNNYLG 519
           L WG G+  V+ QG L T L+ +++AAVT+LDRW V IV++  ++    +  +FM NYLG
Sbjct: 303 LQWGRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKFMMNYLG 362

Query: 520 IGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTYADIPWQVRVEVDGVE 579
           IGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGA+ IMDR  AD+PWQV +EVDG +
Sbjct: 363 IGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKD 422

Query: 580 VEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDSFDSQSMHDKILEVVSISGTWHLGKLQ 639
           +E+P+D+EG++V NIGSYMGGVDLW N+ E  D+F  Q MHDK LEVV + G WHLGKLQ
Sbjct: 423 IEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQ 482

Query: 640 VGLSRARRLAQGRSIRIQLCAALPVQIDGEPWFQDIPCTLVISHHGEAFMLKRAVEEPLG 699
           VGLS+ARRLAQG+ IRI + +  PVQIDGEP+ Q   C L I+HHG+ FML+RA +EP G
Sbjct: 483 VGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQPGC-LEITHHGQVFMLRRASDEPRG 542

Query: 700 HAAAIITDVLESAESNNVINASQKRVLLQEIAKRLT 729
           HAAAI+ +VL  AE   VINASQK+VLLQ++A  L+
Sbjct: 543 HAAAIMNEVLLDAECKGVINASQKKVLLQQMALHLS 558

BLAST of Carg04750 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 156.4 bits (394), Expect = 8.9e-38
Identity = 110/377 (29.18%), Postives = 168/377 (44.56%), Query Frame = 0

Query: 360 PLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELS------------------SAQGPES 419
           PL+VF+N KSG R+G  +K+RL  L++  QV++L+                  +++G E 
Sbjct: 90  PLMVFVNPKSGGRQGPLIKERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDEC 149

Query: 420 GLYLFRKVPHFKVLVCGGDGTVGWVLNCIDK---QNFVSPPPVAILPAGTGNDLARVLNW 479
              +  K+   +++V GGDGTVGWVL C+ +   QN +  PPV+I+P GTGNDL+R   W
Sbjct: 150 AKEIREKM---RIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGW 209

Query: 480 GGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN---------- 539
           GG       +  +   L     A ++ LD W + I    G+ +  P  +           
Sbjct: 210 GGSF-PFAWKSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQN 269

Query: 540 -------------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA 599
                              NY  IG DA+VA   H+LR E P        NK++Y+  G 
Sbjct: 270 LEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGC 329

Query: 600 KS-------IMD---RTYADIPWQVRVEVDGVE---VEVPEDAEGVLVANIGSYMGGVDL 659
                    I D   R   +I      ++D  E   V VP+    V+  N+ SY  G + 
Sbjct: 330 SQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNP 389

Query: 660 WHNEDETF---DSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSIRIQLCA 667
           W N  + +     F      D +LE+  +   WH   + V L  A+ +AQ  +IR+++  
Sbjct: 390 WGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRG 449

BLAST of Carg04750 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 153.7 bits (387), Expect = 5.8e-37
Identity = 107/383 (27.94%), Postives = 161/383 (42.04%), Query Frame = 0

Query: 351 VIELPPDSRPLLVFINKKSGARRGDSLKQRLNMLLNPVQVFELSSAQGPE---SGLYLFR 410
           V ++ P + P++VFIN  SG R G  LK+RL  L++  QVF+L+  +  E    GL    
Sbjct: 83  VADMAPHA-PMVVFINPNSGGRHGPVLKERLQQLMSEEQVFDLTEVKPHEFVRYGLGCLE 142

Query: 411 KV------------PHFKVLVCGGDGTVGWVLNC---IDKQNFVSPPPVAILPAGTGNDL 470
           KV               +++V GGDGTVGWVL C   ++K      PPV ++P GTGNDL
Sbjct: 143 KVAAEGDECAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVIPLGTGNDL 202

Query: 471 ARVLNWGGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN---- 530
           +R   WGG      R   +   L       V  LD WK+ +    G+ +  P  +     
Sbjct: 203 SRSFGWGGSFPFAWR-SAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPPYSLKPAEE 262

Query: 531 -------------------------NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVL 590
                                    NYL IG DA+VA   H+LR   P        NK++
Sbjct: 263 NELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQGPISNKII 322

Query: 591 YAREGAKS-------IMDRTYADIPWQVRVEVDGV------EVEVPEDAEGVLVANIGSY 650
           Y+  G          + D     +   +++ +  V      E+ VP++   ++  N+ SY
Sbjct: 323 YSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSIVALNLHSY 382

Query: 651 MGGVDLWHNEDETF---DSFDSQSMHDKILEVVSISGTWHLGKLQVGLSRARRLAQGRSI 667
             G   W N    +     F      D ++E+      WH   +   L  A+ +AQ  ++
Sbjct: 383 GSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAKHIAQAAAV 442