Carg04688 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04688
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	nudix hydrolase 18, mitochondrial-like
Location	Carg_Chr13: 9800364 .. 9801572 (-)
RNA-Seq Expression	Carg04688
Synteny	Carg04688

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTCTTTCTTTCCAGAAATATTGTAAATTTTCTTATCTCTTCCCTCTTCTCATCCAAGAAAATCCCTACGAATCTCGTCCCTTCTCAGATTGAAAACATGGTCTCCATGGTGTCAGCCCGTACCGGAAGACACAGGCAGCGCTTCAATAAAGAGTATCGCCTGGTTGTAGGGTATGTTTCTTCCTTTCGTTTTTTCCCCTTTCTTTTTGGGCTTGTTAAGAAAATGTTGAAATTTTACGTGCCATGAAGCCCTCTCAATTGTGGATTTCTCAAATTTTGTATTTCTAGGGGTAGTTTGGATGGTTAATTAATTAAACACCATCTTTTACGCGTTGTTATGAGGCTCTCATTTGCAAATTGTGTATCGTTCCTTTTATCTTGGGATTTCTTTTGTAGCCAGGAAAATCTTATCGATTTCTTGATTTACTATTAATCCCCGGACTTTTTCTTGGGGGTTTTCTGTTTTCAGATGCATTCCTTACAGATACAAAGACGCCCAGAAATCGTTTTCGGTTGACAATATAGAAGTTCTTGTGATTAGTTCGCAGAAAGGGCATGCAATGTTGTTCCCGAAGGTACCATTTTAGGCCGAATGAAAGTGTTGAACATTTGGTCGTGGATCATTCACCCTCTCTCTTGTATACTCAATATTTTTTCATTCGAAAATGCAGGGTGGGTGGGAAAACGACGAGTCAATGGAAACCGCAGCTTCAAGAGAGACATTCGAGGAAGCTGGAGTGACTGGCAACATCGAAGTTAGTATTTCGAATCTAGGATTCCATAATTGGTTGTGTAGCGCAAGTTTTGTTCATCCAAATTCAAATTAAATTTTATTTTACAGAGCAGATTAGGCAAATGGTATTACAAGAGCAAGAGGCATGCGAAGATGCATGAAGCCTACATGTTCGCTTTGCGTGTAACGAAACAACTGGAAAATTGGCCGGAGAAAGATATCCGAAAGCGAGAATGGGTGAGTCTGTCTCAACTCCACTACCTGCCTCGGGTATCTAAATATTGTTCCAAGGTAGAAAGTGTTCTTATCATCATCTCTTACTAACTGAATTTCCTATGGAACTTGTAGATGAGTGTGGAGAAAGCCAGAGAAGTTTGCCCTCACATGTGGATGAAAGAGGCTTTAGATTTATTACTCATACGACAATCAGAAGTTGAGCAGAACTTGGATGACGAAACGCCTTGTTCATAA

mRNA sequence

ATGGGTCTCTTTCTTTCCAGAAATATTGTAAATTTTCTTATCTCTTCCCTCTTCTCATCCAAGAAAATCCCTACGAATCTCGTCCCTTCTCAGATTGAAAACATGGTCTCCATGGTGTCAGCCCGTACCGGAAGACACAGGCAGCGCTTCAATAAAGAGTATCGCCTGGTTGTAGGATGCATTCCTTACAGATACAAAGACGCCCAGAAATCGTTTTCGGTTGACAATATAGAAGTTCTTGTGATTAGTTCGCAGAAAGGGCATGCAATGTTGTTCCCGAAGGGTGGGTGGGAAAACGACGAGTCAATGGAAACCGCAGCTTCAAGAGAGACATTCGAGGAAGCTGGAGTGACTGGCAACATCGAAAGCAGATTAGGCAAATGGTATTACAAGAGCAAGAGGCATGCGAAGATGCATGAAGCCTACATGTTCGCTTTGCGTGTAACGAAACAACTGGAAAATTGGCCGGAGAAAGATATCCGAAAGCGAGAATGGATGAGTGTGGAGAAAGCCAGAGAAGTTTGCCCTCACATGTGGATGAAAGAGGCTTTAGATTTATTACTCATACGACAATCAGAAGTTGAGCAGAACTTGGATGACGAAACGCCTTGTTCATAA

Coding sequence (CDS)

ATGGGTCTCTTTCTTTCCAGAAATATTGTAAATTTTCTTATCTCTTCCCTCTTCTCATCCAAGAAAATCCCTACGAATCTCGTCCCTTCTCAGATTGAAAACATGGTCTCCATGGTGTCAGCCCGTACCGGAAGACACAGGCAGCGCTTCAATAAAGAGTATCGCCTGGTTGTAGGATGCATTCCTTACAGATACAAAGACGCCCAGAAATCGTTTTCGGTTGACAATATAGAAGTTCTTGTGATTAGTTCGCAGAAAGGGCATGCAATGTTGTTCCCGAAGGGTGGGTGGGAAAACGACGAGTCAATGGAAACCGCAGCTTCAAGAGAGACATTCGAGGAAGCTGGAGTGACTGGCAACATCGAAAGCAGATTAGGCAAATGGTATTACAAGAGCAAGAGGCATGCGAAGATGCATGAAGCCTACATGTTCGCTTTGCGTGTAACGAAACAACTGGAAAATTGGCCGGAGAAAGATATCCGAAAGCGAGAATGGATGAGTGTGGAGAAAGCCAGAGAAGTTTGCCCTCACATGTGGATGAAAGAGGCTTTAGATTTATTACTCATACGACAATCAGAAGTTGAGCAGAACTTGGATGACGAAACGCCTTGTTCATAA

Protein sequence

MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIRQSEVEQNLDDETPCS

Homology

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BLAST of Carg04688 vs. NCBI nr
Match: XP_022923899.1 (nudix hydrolase 18, mitochondrial-like [Cucurbita moschata] >XP_023519987.1 nudix hydrolase 18, mitochondrial-like [Cucurbita pepo subsp. pepo] >KAG7020068.1 Nudix hydrolase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 413.3 bits (1061), Expect = 1.2e-111
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC
Sbjct: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN
Sbjct: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM
Sbjct: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180

Query: 181 KEALDLLLIRQSEVEQNLDDETPCS 206
           KEALDLLLIRQSEVEQNLDDETPCS
Sbjct: 181 KEALDLLLIRQSEVEQNLDDETPCS 205

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BLAST of Carg04688 vs. NCBI nr
Match: XP_023001154.1 (nudix hydrolase 18, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 407.5 bits (1046), Expect = 6.7e-110
Identity = 202/205 (98.54%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGR+RQR+NKEYRLVVGC
Sbjct: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRYRQRYNKEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN
Sbjct: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IESRLGKWYYKSK HAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM
Sbjct: 121 IESRLGKWYYKSKSHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180

Query: 181 KEALDLLLIRQSEVEQNLDDETPCS 206
           KEALDLLLIRQSEVEQNLDDETPCS
Sbjct: 181 KEALDLLLIRQSEVEQNLDDETPCS 205

[Close]

BLAST of Carg04688 vs. NCBI nr
Match: KAG6584480.1 (UV-B-induced protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 333.2 bits (853), Expect = 1.6e-87
Identity = 165/171 (96.49%), Postives = 168/171 (98.25%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC
Sbjct: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN
Sbjct: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKA 172
           IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREW  + ++
Sbjct: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWFLLPRS 171

[Close]

BLAST of Carg04688 vs. NCBI nr
Match: XP_022998739.1 (nudix hydrolase 18, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 320.5 bits (820), Expect = 1.1e-83
Identity = 163/206 (79.13%), Postives = 179/206 (86.89%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLF SRNI NFL+SS FSS+K       SQIEN+VS+VSARTGRHRQR+N+EYRLVVGC
Sbjct: 1   MGLFFSRNIANFLLSSFFSSQKF-----TSQIENVVSVVSARTGRHRQRYNQEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDA KSFSVDNIEVL ISSQKG AM+FPKGGWENDESMETAA RET EEAGV GN
Sbjct: 61  IPYRYKDALKSFSVDNIEVLGISSQKGQAMMFPKGGWENDESMETAALRETLEEAGVVGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IE RLGKWYYKSK  A MHEAYMF L+VTK LENWPEKD R+REWMSVEKAREVCP  WM
Sbjct: 121 IEKRLGKWYYKSKNQATMHEAYMFPLQVTKLLENWPEKDFRRREWMSVEKAREVCP-QWM 180

Query: 181 KEALDLLLIRQSEVEQNLD-DETPCS 206
           KEALDLL+ +++E+EQ+LD +ETPCS
Sbjct: 181 KEALDLLISQRTELEQSLDEEETPCS 200

[Close]

BLAST of Carg04688 vs. NCBI nr
Match: XP_023521214.1 (nudix hydrolase 18, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023525322.1 nudix hydrolase 18, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.1 bits (819), Expect = 1.4e-83
Identity = 162/206 (78.64%), Postives = 179/206 (86.89%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLF SRNI  FL+SS FSS+K      PSQIEN+VS+VS+RTGRHRQR+N+EYRLVVGC
Sbjct: 1   MGLFFSRNIAKFLLSSFFSSQKF-----PSQIENVVSVVSSRTGRHRQRYNQEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDA KSFSVDNIEVL ISSQKG AM+FPKGGWENDESMETAA RET EEAGV GN
Sbjct: 61  IPYRYKDALKSFSVDNIEVLGISSQKGQAMMFPKGGWENDESMETAALRETLEEAGVVGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IE RLGKWYYKSK  A MHEAYMF L+VTK LENWPEKD R+REWMSVEKAREVCP  WM
Sbjct: 121 IEKRLGKWYYKSKNQATMHEAYMFPLQVTKLLENWPEKDFRRREWMSVEKAREVCP-QWM 180

Query: 181 KEALDLLLIRQSEVEQNLD-DETPCS 206
           KEALDLL+ +++E+EQ+LD +ETPCS
Sbjct: 181 KEALDLLISQRTELEQSLDEEETPCS 200

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BLAST of Carg04688 vs. ExPASy Swiss-Prot
Match: Q9LE73 (Nudix hydrolase 4 OS=Arabidopsis thaliana OX=3702 GN=NUDT4 PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.8e-50
Identity = 105/193 (54.40%), Postives = 143/193 (74.09%), Query Frame = 0


Query: 1   MGLFLSR--NIVNFLISSLFSSKKIP-TNLVPSQIENMVSMVSARTGRHRQRFNKE-YRL 60
           + LF+S   N+ ++L S +F  + IP T +VP+QIE +VS+VS RTGR  QR++   YR 
Sbjct: 6   VSLFVSNLSNVASYL-SPIF--ENIPSTKVVPAQIEKVVSLVS-RTGRDLQRYDHAGYRQ 65

Query: 61  VVGCIPYRYKDAQ-KSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEA 120
           VVGC+PYRYK  +        I+VL++S+QKG  MLFPKGGWE DESME AA RET EEA
Sbjct: 66  VVGCVPYRYKKQEVNGVETQVIQVLLVSAQKGKGMLFPKGGWETDESMEEAALRETIEEA 125

Query: 121 GVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVC 180
           GVTG +E +LGKW YKSKRH+ +H+ YMFAL V+++ E WPE ++R+R W+S+++AREVC
Sbjct: 126 GVTGELEEKLGKWQYKSKRHSIIHDGYMFALLVSQEFERWPEAEMRQRRWVSLDEAREVC 185

Query: 181 PHMWMKEALDLLL 189
            + WM+EAL+  +
Sbjct: 186 QNWWMREALEAFI 194

[Close]

BLAST of Carg04688 vs. ExPASy Swiss-Prot
Match: Q9ZU95 (Nudix hydrolase 17, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NUDT17 PE=2 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.1e-46
Identity = 96/172 (55.81%), Postives = 123/172 (71.51%), Query Frame = 0


Query: 32  IENMVSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFSVDNIEVLVISSQKGHAML 91
           +E MV + S RTGR  QR+NK  R VVGC+PYR+K +      D +EVLVISSQKGHA++
Sbjct: 3   VEKMVCLAS-RTGRQFQRYNKGRRQVVGCVPYRFKLSNDGKISDEVEVLVISSQKGHALM 62

Query: 92  FPKGGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQ 151
           FPKGGWE DES+E AASRE  EEAGV GN+E +LGKW + SK     +E  MF + VT+Q
Sbjct: 63  FPKGGWELDESVEEAASRECLEEAGVLGNVEHQLGKWDFLSKSRGTYYEGLMFPMLVTEQ 122

Query: 152 LENWPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIR-QSEVEQNLDDET 203
           LE WPE+ +R+R WM+V +ARE C   WMKEALD+L+ R  S + Q  +++T
Sbjct: 123 LELWPEQHVRQRIWMNVTEAREACRDWWMKEALDVLVERLSSPMNQPKEEKT 173

[Close]

BLAST of Carg04688 vs. ExPASy Swiss-Prot
Match: Q9LQU5 (Nudix hydrolase 18, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NUDT18 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.2e-45
Identity = 92/158 (58.23%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 35  MVSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPK 94
           MV +VS RTGR  QR+NK  R VVGCIPYR K +      D  EVLVISSQKGHA++FPK
Sbjct: 1   MVCLVS-RTGRQSQRYNKGRRQVVGCIPYRLKISSDGTISDEFEVLVISSQKGHALMFPK 60

Query: 95  GGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQLEN 154
           GGWE DES+E AASRE+ EEAGV GN+E +LGKW + SK     +E +MF + V ++LE 
Sbjct: 61  GGWELDESVEEAASRESLEEAGVVGNVERQLGKWDFLSKSKGTFYEGFMFPMLVKEELEL 120

Query: 155 WPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIRQS 193
           WPE+ +R+R WM V++AR+ C   WMKEALD+L+ R S
Sbjct: 121 WPEQHLRQRIWMKVDEARDACRDWWMKEALDVLVQRLS 157

[Close]

BLAST of Carg04688 vs. ExPASy Swiss-Prot
Match: Q8VY81 (Nudix hydrolase 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT21 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 8.7e-44
Identity = 96/178 (53.93%), Postives = 124/178 (69.66%), Query Frame = 0


Query: 20  SKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKE-YRLVVGCIPYRYKDAQKSFSVDNIE 79
           SKKI    VP+  E +VS+VS RTGR  QR+N   YR VVGC+PYRY    K      IE
Sbjct: 28  SKKIVP--VPTPSEKVVSLVS-RTGRDLQRYNTAGYRQVVGCVPYRY----KKHGGGEIE 87

Query: 80  VLVISSQ-KGHAMLFPKGGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAK 139
           VL+IS+Q KG  ML PKGGWE DES+E AA RET EEAGVTG +E  LGKW YKSKRH  
Sbjct: 88  VLLISAQKKGKGMLLPKGGWEIDESIEEAALRETIEEAGVTGQLEESLGKWQYKSKRHTM 147

Query: 140 MHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIRQSEVE 196
           +H+ +MF L V++Q E WPE + R+R+W+S+ +A E+C + WM+EAL+  + R+ + +
Sbjct: 148 IHDGHMFPLLVSQQFEIWPESEFRQRKWVSLSEAIELCQNSWMREALEAFINRKCQTQ 198

[Close]

BLAST of Carg04688 vs. ExPASy Swiss-Prot
Match: Q93ZY7 (Nudix hydrolase 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NUDT12 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.3e-35
Identity = 80/181 (44.20%), Postives = 119/181 (65.75%), Query Frame = 0


Query: 36  VSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFS------VDNIEVLVISSQKGHA 95
           +S++S+RTGR RQR++  +RLV GCIPYR   A ++        V+ +EVL++SS   H 
Sbjct: 1   MSVLSSRTGRDRQRYDNNFRLVSGCIPYRLMKADETEEDSGVDFVNKLEVLMVSSPNRHD 60

Query: 96  MLFPKGGWENDESMETAASRETFEEAGVTGNI-ESRLGKWYYKSKRHAKMHE------AY 155
           ++FPKGGWE+DE++  AASRE  EEAGV G + E  LG W ++SK      E       Y
Sbjct: 61  LVFPKGGWEDDETVLEAASREAIEEAGVKGILRELPLGVWEFRSKSSTVEDECLGGCKGY 120

Query: 156 MFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWMKEAL-DLLLIRQSEVEQNLDDE 203
           MFAL+VT++LE+WPE+  R+R W++V++A E+C + WM+ AL + L + + E     ++E
Sbjct: 121 MFALKVTEELEDWPERKNRERRWLTVKEALELCRYEWMQRALEEFLRVMEDERRLRTEEE 180

[Close]

BLAST of Carg04688 vs. ExPASy TrEMBL
Match: A0A6J1E7N3 (nudix hydrolase 18, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111431483 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 5.9e-112
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC
Sbjct: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN
Sbjct: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM
Sbjct: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180

Query: 181 KEALDLLLIRQSEVEQNLDDETPCS 206
           KEALDLLLIRQSEVEQNLDDETPCS
Sbjct: 181 KEALDLLLIRQSEVEQNLDDETPCS 205

[Close]

BLAST of Carg04688 vs. ExPASy TrEMBL
Match: A0A6J1KLX9 (nudix hydrolase 18, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111495376 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.2e-110
Identity = 202/205 (98.54%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGR+RQR+NKEYRLVVGC
Sbjct: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRYRQRYNKEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN
Sbjct: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IESRLGKWYYKSK HAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM
Sbjct: 121 IESRLGKWYYKSKSHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180

Query: 181 KEALDLLLIRQSEVEQNLDDETPCS 206
           KEALDLLLIRQSEVEQNLDDETPCS
Sbjct: 181 KEALDLLLIRQSEVEQNLDDETPCS 205

[Close]

BLAST of Carg04688 vs. ExPASy TrEMBL
Match: A0A6J1KF58 (nudix hydrolase 18, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111493314 PE=4 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 5.2e-84
Identity = 163/206 (79.13%), Postives = 179/206 (86.89%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLF SRNI NFL+SS FSS+K       SQIEN+VS+VSARTGRHRQR+N+EYRLVVGC
Sbjct: 1   MGLFFSRNIANFLLSSFFSSQKF-----TSQIENVVSVVSARTGRHRQRYNQEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDA KSFSVDNIEVL ISSQKG AM+FPKGGWENDESMETAA RET EEAGV GN
Sbjct: 61  IPYRYKDALKSFSVDNIEVLGISSQKGQAMMFPKGGWENDESMETAALRETLEEAGVVGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IE RLGKWYYKSK  A MHEAYMF L+VTK LENWPEKD R+REWMSVEKAREVCP  WM
Sbjct: 121 IEKRLGKWYYKSKNQATMHEAYMFPLQVTKLLENWPEKDFRRREWMSVEKAREVCP-QWM 180

Query: 181 KEALDLLLIRQSEVEQNLD-DETPCS 206
           KEALDLL+ +++E+EQ+LD +ETPCS
Sbjct: 181 KEALDLLISQRTELEQSLDEEETPCS 200

[Close]

BLAST of Carg04688 vs. ExPASy TrEMBL
Match: A0A6J1GAV3 (nudix hydrolase 18, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111452489 PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.2e-82
Identity = 159/205 (77.56%), Postives = 175/205 (85.37%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLF SRNI  FL+SS FSS+K      PSQIEN+VS+VSARTGRHRQR+N+EYRLVVGC
Sbjct: 1   MGLFFSRNIAKFLLSSFFSSQKF-----PSQIENVVSVVSARTGRHRQRYNQEYRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYKDA KSFSVDNIEVL ISSQKG AM+FPKGGWENDESMETAA RET EEAGV GN
Sbjct: 61  IPYRYKDALKSFSVDNIEVLGISSQKGQAMMFPKGGWENDESMETAALRETLEEAGVVGN 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           IE RLGKWYYKSK  A MHEAYMF L+VTK LENWPEKD R+REWMSVEKAREVCP  WM
Sbjct: 121 IEKRLGKWYYKSKNQATMHEAYMFPLQVTKLLENWPEKDFRRREWMSVEKAREVCP-QWM 180

Query: 181 KEALDLLLIRQSEVEQNLDDETPCS 206
           KEALDLL+ +++E+E   ++ TPCS
Sbjct: 181 KEALDLLISQRTELESLDEEGTPCS 199

[Close]

BLAST of Carg04688 vs. ExPASy TrEMBL
Match: A0A1J7HT24 (Nudix hydrolase domain-containing protein OS=Lupinus angustifolius OX=3871 GN=TanjilG_06128 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.1e-62
Identity = 122/198 (61.62%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 1   MGLFLSRNIVNFLISSLFSSKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKEYRLVVGC 60
           MGLFLSRN+ +  +     SKK P +L P Q+ENM+ +VS+RTGRH QR++K +RLVVGC
Sbjct: 1   MGLFLSRNLASHFLYPFLFSKKFPEDLFPKQLENMMCLVSSRTGRHLQRYDKGHRLVVGC 60

Query: 61  IPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEAGVTGN 120
           IPYRYK          IEVLVIS+QKG  M FPKGGWE+DESME AA RET EEAGV G 
Sbjct: 61  IPYRYK--SNGSQDKEIEVLVISAQKGQGMQFPKGGWESDESMEQAALRETVEEAGVVGK 120

Query: 121 IESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWM 180
           +ES+LGKW+YKSK  A MHE YMF L V KQL+NWPE + RKR WM+V +A+E+CPH WM
Sbjct: 121 VESKLGKWFYKSKSQAIMHEGYMFPLLVKKQLDNWPEMNFRKRRWMTVPEAKEICPHTWM 180

Query: 181 KEALDLLLIRQSEVEQNL 199
           KEALD+L+ RQ + +  L
Sbjct: 181 KEALDVLVSRQIQPQPKL 196

[Close]

BLAST of Carg04688 vs. TAIR 10
Match: AT1G18300.1 (nudix hydrolase homolog 4 )

HSP 1 Score: 199.9 bits (507), Expect = 2.0e-51
Identity = 105/193 (54.40%), Postives = 143/193 (74.09%), Query Frame = 0


Query: 1   MGLFLSR--NIVNFLISSLFSSKKIP-TNLVPSQIENMVSMVSARTGRHRQRFNKE-YRL 60
           + LF+S   N+ ++L S +F  + IP T +VP+QIE +VS+VS RTGR  QR++   YR 
Sbjct: 6   VSLFVSNLSNVASYL-SPIF--ENIPSTKVVPAQIEKVVSLVS-RTGRDLQRYDHAGYRQ 65

Query: 61  VVGCIPYRYKDAQ-KSFSVDNIEVLVISSQKGHAMLFPKGGWENDESMETAASRETFEEA 120
           VVGC+PYRYK  +        I+VL++S+QKG  MLFPKGGWE DESME AA RET EEA
Sbjct: 66  VVGCVPYRYKKQEVNGVETQVIQVLLVSAQKGKGMLFPKGGWETDESMEEAALRETIEEA 125

Query: 121 GVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVC 180
           GVTG +E +LGKW YKSKRH+ +H+ YMFAL V+++ E WPE ++R+R W+S+++AREVC
Sbjct: 126 GVTGELEEKLGKWQYKSKRHSIIHDGYMFALLVSQEFERWPEAEMRQRRWVSLDEAREVC 185

Query: 181 PHMWMKEALDLLL 189
            + WM+EAL+  +
Sbjct: 186 QNWWMREALEAFI 194

[Close]

BLAST of Carg04688 vs. TAIR 10
Match: AT2G01670.1 (nudix hydrolase homolog 17 )

HSP 1 Score: 188.0 bits (476), Expect = 7.8e-48
Identity = 96/172 (55.81%), Postives = 123/172 (71.51%), Query Frame = 0


Query: 32  IENMVSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFSVDNIEVLVISSQKGHAML 91
           +E MV + S RTGR  QR+NK  R VVGC+PYR+K +      D +EVLVISSQKGHA++
Sbjct: 3   VEKMVCLAS-RTGRQFQRYNKGRRQVVGCVPYRFKLSNDGKISDEVEVLVISSQKGHALM 62

Query: 92  FPKGGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQ 151
           FPKGGWE DES+E AASRE  EEAGV GN+E +LGKW + SK     +E  MF + VT+Q
Sbjct: 63  FPKGGWELDESVEEAASRECLEEAGVLGNVEHQLGKWDFLSKSRGTYYEGLMFPMLVTEQ 122

Query: 152 LENWPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIR-QSEVEQNLDDET 203
           LE WPE+ +R+R WM+V +ARE C   WMKEALD+L+ R  S + Q  +++T
Sbjct: 123 LELWPEQHVRQRIWMNVTEAREACRDWWMKEALDVLVERLSSPMNQPKEEKT 173

[Close]

BLAST of Carg04688 vs. TAIR 10
Match: AT1G14860.1 (nudix hydrolase homolog 18 )

HSP 1 Score: 184.5 bits (467), Expect = 8.6e-47
Identity = 92/158 (58.23%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 35  MVSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFSVDNIEVLVISSQKGHAMLFPK 94
           MV +VS RTGR  QR+NK  R VVGCIPYR K +      D  EVLVISSQKGHA++FPK
Sbjct: 1   MVCLVS-RTGRQSQRYNKGRRQVVGCIPYRLKISSDGTISDEFEVLVISSQKGHALMFPK 60

Query: 95  GGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAKMHEAYMFALRVTKQLEN 154
           GGWE DES+E AASRE+ EEAGV GN+E +LGKW + SK     +E +MF + V ++LE 
Sbjct: 61  GGWELDESVEEAASRESLEEAGVVGNVERQLGKWDFLSKSKGTFYEGFMFPMLVKEELEL 120

Query: 155 WPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIRQS 193
           WPE+ +R+R WM V++AR+ C   WMKEALD+L+ R S
Sbjct: 121 WPEQHLRQRIWMKVDEARDACRDWWMKEALDVLVQRLS 157

[Close]

BLAST of Carg04688 vs. TAIR 10
Match: AT1G73540.1 (nudix hydrolase homolog 21 )

HSP 1 Score: 178.3 bits (451), Expect = 6.2e-45
Identity = 96/178 (53.93%), Postives = 124/178 (69.66%), Query Frame = 0


Query: 20  SKKIPTNLVPSQIENMVSMVSARTGRHRQRFNKE-YRLVVGCIPYRYKDAQKSFSVDNIE 79
           SKKI    VP+  E +VS+VS RTGR  QR+N   YR VVGC+PYRY    K      IE
Sbjct: 28  SKKIVP--VPTPSEKVVSLVS-RTGRDLQRYNTAGYRQVVGCVPYRY----KKHGGGEIE 87

Query: 80  VLVISSQ-KGHAMLFPKGGWENDESMETAASRETFEEAGVTGNIESRLGKWYYKSKRHAK 139
           VL+IS+Q KG  ML PKGGWE DES+E AA RET EEAGVTG +E  LGKW YKSKRH  
Sbjct: 88  VLLISAQKKGKGMLLPKGGWEIDESIEEAALRETIEEAGVTGQLEESLGKWQYKSKRHTM 147

Query: 140 MHEAYMFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWMKEALDLLLIRQSEVE 196
           +H+ +MF L V++Q E WPE + R+R+W+S+ +A E+C + WM+EAL+  + R+ + +
Sbjct: 148 IHDGHMFPLLVSQQFEIWPESEFRQRKWVSLSEAIELCQNSWMREALEAFINRKCQTQ 198

[Close]

BLAST of Carg04688 vs. TAIR 10
Match: AT1G12880.1 (nudix hydrolase homolog 12 )

HSP 1 Score: 149.4 bits (376), Expect = 3.1e-36
Identity = 80/181 (44.20%), Postives = 119/181 (65.75%), Query Frame = 0


Query: 36  VSMVSARTGRHRQRFNKEYRLVVGCIPYRYKDAQKSFS------VDNIEVLVISSQKGHA 95
           +S++S+RTGR RQR++  +RLV GCIPYR   A ++        V+ +EVL++SS   H 
Sbjct: 1   MSVLSSRTGRDRQRYDNNFRLVSGCIPYRLMKADETEEDSGVDFVNKLEVLMVSSPNRHD 60

Query: 96  MLFPKGGWENDESMETAASRETFEEAGVTGNI-ESRLGKWYYKSKRHAKMHE------AY 155
           ++FPKGGWE+DE++  AASRE  EEAGV G + E  LG W ++SK      E       Y
Sbjct: 61  LVFPKGGWEDDETVLEAASREAIEEAGVKGILRELPLGVWEFRSKSSTVEDECLGGCKGY 120

Query: 156 MFALRVTKQLENWPEKDIRKREWMSVEKAREVCPHMWMKEAL-DLLLIRQSEVEQNLDDE 203
           MFAL+VT++LE+WPE+  R+R W++V++A E+C + WM+ AL + L + + E     ++E
Sbjct: 121 MFALKVTEELEDWPERKNRERRWLTVKEALELCRYEWMQRALEEFLRVMEDERRLRTEEE 180

The following BLAST results are available for this feature:

BLAST of Carg04688 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923899.1	1.2e-111	100.00	nudix hydrolase 18, mitochondrial-like [Cucurbita moschata] >XP_023519987.1 nudi...	[more]
XP_023001154.1	6.7e-110	98.54	nudix hydrolase 18, mitochondrial-like [Cucurbita maxima]	[more]
KAG6584480.1	1.6e-87	96.49	UV-B-induced protein, chloroplastic, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022998739.1	1.1e-83	79.13	nudix hydrolase 18, mitochondrial-like [Cucurbita maxima]	[more]
XP_023521214.1	1.4e-83	78.64	nudix hydrolase 18, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_02352532...	[more]

BLAST of Carg04688 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LE73	2.8e-50	54.40	Nudix hydrolase 4 OS=Arabidopsis thaliana OX=3702 GN=NUDT4 PE=1 SV=1	[more]
Q9ZU95	1.1e-46	55.81	Nudix hydrolase 17, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NUDT17 PE=2...	[more]
Q9LQU5	1.2e-45	58.23	Nudix hydrolase 18, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NUDT18 PE=2...	[more]
Q8VY81	8.7e-44	53.93	Nudix hydrolase 21, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT21 PE=2...	[more]
Q93ZY7	4.3e-35	44.20	Nudix hydrolase 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=NUDT12 PE=2...	[more]

BLAST of Carg04688 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E7N3	5.9e-112	100.00	nudix hydrolase 18, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1KLX9	3.2e-110	98.54	nudix hydrolase 18, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC1114953...	[more]
A0A6J1KF58	5.2e-84	79.13	nudix hydrolase 18, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC1114933...	[more]
A0A6J1GAV3	2.2e-82	77.56	nudix hydrolase 18, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A1J7HT24	1.1e-62	61.62	Nudix hydrolase domain-containing protein OS=Lupinus angustifolius OX=3871 GN=Ta...	[more]

BLAST of Carg04688 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18300.1	2.0e-51	54.40	nudix hydrolase homolog 4	[more]
AT2G01670.1	7.8e-48	55.81	nudix hydrolase homolog 17	[more]
AT1G14860.1	8.6e-47	58.23	nudix hydrolase homolog 18	[more]
AT1G73540.1	6.2e-45	53.93	nudix hydrolase homolog 21	[more]
AT1G12880.1	3.1e-36	44.20	nudix hydrolase homolog 12	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000086	NUDIX hydrolase domain	PFAM	PF00293	NUDIX	coord: 79..171 e-value: 1.0E-13 score: 51.5
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 53..188 score: 14.986631
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 38..190 e-value: 1.1E-34 score: 121.2
None	No IPR available	PANTHER	PTHR12629:SF65	NUDIX HYDROLASE 4-LIKE	coord: 15..196
None	No IPR available	PANTHER	PTHR12629	DIPHOSPHOINOSITOL POLYPHOSPHATE PHOSPHOHYDROLASE	coord: 15..196
None	No IPR available	CDD	cd04666	Nudix_Hydrolase_9	coord: 56..181 e-value: 6.4164E-45 score: 143.525
IPR020084	NUDIX hydrolase, conserved site	PROSITE	PS00893	NUDIX_BOX	coord: 95..116
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 40..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04688-RA	Carg04688-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016787	hydrolase activity