Carg04635 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04635
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPLASMODESMATA CALLOSE-BINDING PROTEIN 5-like
LocationCarg_Chr11: 12802289 .. 12803136 (-)
RNA-Seq ExpressionCarg04635
SyntenyCarg04635
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTGTTCCCTCTTAAACCTCCCCATTTTCTTTCTCTCTCTAAAACGCCTCTCTCCTACCAATGGAGATCGATTTAGCTCTTTTTCTCCTCATTGCCCTGATTATCTGCAGCGCGGCGGAGCCCCAGCATCCGTCGATCAAAGGCCAAGGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTGCAATCCGCACTCGACTGGGCCTGCGGCGCTGGCGGCTCCGACTGTTCCCCAATCCAGCGTAGTGGCGCCTGCTACGACGCCTCCAATGTCCAGAACATGGCCTCCTTCGCCTTCAACGACTACTTCCTCAAACACGGCATGACTGACGATAGTTGCTTCTTTCAAAATTCCGCCGCTATCACTTCCTTAAACCCTAGTCAGTTTCTTCTCCCTCCCGATCTCTCAAATTCTCCATCGAATCTTTGTTTTTCTTTCTAGATCTGTAACTGAATCGCTCATTGATTTGATCTTTCTTGTTTCAGGCTATGGAAATTGCAGATTCCCATCTAGGTAAAAACTTTCTCCATGATCTTTAAACACCCTGTTTCGATTTAGGGAACAATTGATGTCCGTAATCAAGAAATTAATCATCAACCGTCAACGAATCTCCGACTTTAAAGCCTTTTTTTCCAGTTTTAAAGTGATCGATTTCTCTCATTTATGGCGCAGTTCGTTGAGAAATGGGAACTTCTCAAGCCAATCCACATCCGTAGGGCTGGGGCCAAGCGAAGACCTAAGCGGATCGGGTCGAAAATCGGGTCGGATATGGGTTTGGCCCATAGCAATCGGGTATTTGTTCTTTAAAATTATGTTTCTAATTTAA

mRNA sequence

CTCTGTTCCCTCTTAAACCTCCCCATTTTCTTTCTCTCTCTAAAACGCCTCTCTCCTACCAATGGAGATCGATTTAGCTCTTTTTCTCCTCATTGCCCTGATTATCTGCAGCGCGGCGGAGCCCCAGCATCCGTCGATCAAAGGCCAAGGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTGCAATCCGCACTCGACTGGGCCTGCGGCGCTGGCGGCTCCGACTGTTCCCCAATCCAGCGTAGTGGCGCCTGCTACGACGCCTCCAATGTCCAGAACATGGCCTCCTTCGCCTTCAACGACTACTTCCTCAAACACGGCATGACTGACGATAGTTGCTTCTTTCAAAATTCCGCCGCTATCACTTCCTTAAACCCTAGCTATGGAAATTGCAGATTCCCATCTAGTTCGTTGAGAAATGGGAACTTCTCAAGCCAATCCACATCCGTAGGGCTGGGGCCAAGCGAAGACCTAAGCGGATCGGGTCGAAAATCGGGTCGGATATGGGTTTGGCCCATAGCAATCGGGTATTTGTTCTTTAAAATTATGTTTCTAATTTAA

Coding sequence (CDS)

ATGGAGATCGATTTAGCTCTTTTTCTCCTCATTGCCCTGATTATCTGCAGCGCGGCGGAGCCCCAGCATCCGTCGATCAAAGGCCAAGGCGGCGCGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGACGACGCCTCTCTGCAATCCGCACTCGACTGGGCCTGCGGCGCTGGCGGCTCCGACTGTTCCCCAATCCAGCGTAGTGGCGCCTGCTACGACGCCTCCAATGTCCAGAACATGGCCTCCTTCGCCTTCAACGACTACTTCCTCAAACACGGCATGACTGACGATAGTTGCTTCTTTCAAAATTCCGCCGCTATCACTTCCTTAAACCCTAGCTATGGAAATTGCAGATTCCCATCTAGTTCGTTGAGAAATGGGAACTTCTCAAGCCAATCCACATCCGTAGGGCTGGGGCCAAGCGAAGACCTAAGCGGATCGGGTCGAAAATCGGGTCGGATATGGGTTTGGCCCATAGCAATCGGGTATTTGTTCTTTAAAATTATGTTTCTAATTTAA

Protein sequence

MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI
Homology
[Close]
BLAST of Carg04635 vs. NCBI nr
Match: KAG7022965.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 348.6 bits (893), Expect = 3.1e-92
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 174
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 173

[Close]
BLAST of Carg04635 vs. NCBI nr
Match: XP_022930875.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 341.7 bits (875), Expect = 3.8e-90
Identity = 170/173 (98.27%), Postives = 171/173 (98.84%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           SDCSPIQ SGACYDAS+VQ MASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  SDCSPIQPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 174
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 173

[Close]
BLAST of Carg04635 vs. NCBI nr
Match: XP_022989094.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 330.1 bits (845), Expect = 1.1e-86
Identity = 162/173 (93.64%), Postives = 167/173 (96.53%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           M I+ ALFLLI  I+CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MPINFALFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           +DCSPIQ SGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAA+TSLNPSYGNCRF
Sbjct: 61  ADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 174
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGR+S RIWVWPIAIGYLFFKIMFLI
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIGYLFFKIMFLI 173

[Close]
BLAST of Carg04635 vs. NCBI nr
Match: KAG6589266.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 329.3 bits (843), Expect = 2.0e-86
Identity = 163/165 (98.79%), Postives = 163/165 (98.79%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           SDCSPIQ SGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  SDCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYL 166
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIG L
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGLL 165

[Close]
BLAST of Carg04635 vs. NCBI nr
Match: XP_023529864.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 327.4 bits (838), Expect = 7.4e-86
Identity = 160/173 (92.49%), Postives = 167/173 (96.53%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           M I+ ALFLLI LI+CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MAINFALFLLIVLILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           ++CSPIQ SGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  AECSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 174
           PSSSLRNGNFSSQSTSVGLGPSE+LSGSGR+SGRIWVWPIAIGY FFK+ FLI
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSENLSGSGRRSGRIWVWPIAIGYFFFKLTFLI 173

[Close]
BLAST of Carg04635 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 3.1e-34
Identity = 71/132 (53.79%), Postives = 94/132 (71.21%), Query Frame = 0 

Query: 26  IKGQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFA 85
           I GQ    ELWCVAKNNA+D+SLQ+A++WACG GG+DC PIQ+ G C D ++VQ MASF 
Sbjct: 31  IGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFV 90

Query: 86  FNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSSQSTSVGLGPSEDL 145
           FN+Y+LK+G  D++C F N+AA+TSLNPS G C++PSS   N    +  TS+G G + D+
Sbjct: 91  FNNYYLKNGEEDEACNFNNNAALTSLNPSQGTCKYPSSKGANNGRLADDTSMGAGQA-DM 150

Query: 146 SGSGRKSGRIWV 158
           S  GR     W+
Sbjct: 151 SRGGRPISSSWM 161

[Close]
BLAST of Carg04635 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 93.2 bits (230), Expect = 3.1e-18
Identity = 51/116 (43.97%), Postives = 70/116 (60.34%), Query Frame = 0 

Query: 34  ELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFAFNDYFLKH 93
           + +C+A +  D  +LQ+ALDWACG G S+CS IQ   +CY  +NV+  ASFAFN Y+ K 
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 94  GMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSS---QSTSVGLGPSEDLS 147
           G    SC F+  A IT+ +PS+G+C FP S  + GN +     ST V  G +   S
Sbjct: 439 GRASGSCDFKGVAMITTTDPSHGSCIFPGSK-KVGNRTQTVVNSTEVAAGEATSRS 493

[Close]
BLAST of Carg04635 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.3e-16
Identity = 40/101 (39.60%), Postives = 58/101 (57.43%), Query Frame = 0 

Query: 28  GQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFAFN 87
           G GG T+ WC+A + A    LQ+ALDWACG G  DCS +Q    C++   V + AS+AFN
Sbjct: 383 GGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFN 442

Query: 88  DYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNG 129
            Y+ + G +   C F  ++     +PSYGNC +  +   +G
Sbjct: 443 TYYQQSGASSIDCSFNGASVEVDKDPSYGNCLYMIAPATDG 483

[Close]
BLAST of Carg04635 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 1.7e-16
Identity = 48/139 (34.53%), Postives = 74/139 (53.24%), Query Frame = 0 

Query: 25  SIKGQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASF 84
           S+       + +C A+  AD   LQ+ALDWACG G  DCSPI++   CY+  NV   A++
Sbjct: 357 SVLANDTTNQTYCTAREGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANY 416

Query: 85  AFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSSQSTSVGLGPSED 144
           AF+ Y+ + G   D+C F   A+IT+ +PS+G C F  S  R    +  S ++    +  
Sbjct: 417 AFDTYYHQTGNNPDACNFNGVASITTTDPSHGTCVFAGS--RGNGRNGTSVNITAPSANS 476

Query: 145 LSGSGRKSGRIW---VWPI 161
            + SG +S   +   +W I
Sbjct: 477 TTSSGIRSDLYYSRGIWSI 493

[Close]
BLAST of Carg04635 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.2e-16
Identity = 45/111 (40.54%), Postives = 66/111 (59.46%), Query Frame = 0 

Query: 30  GGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFAFNDY 89
           G  +  WCV K    D+ LQ  LD+ACG  G+DC+P    G+C++  NV+   ++A N +
Sbjct: 15  GHTSASWCVCKTGLSDSVLQKTLDYACG-NGADCNPTHPKGSCFNPDNVRAHCNYAVNSF 74

Query: 90  FLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSSQSTSVGLG 141
           F K G   +SC F  +A +T+ +PSY  C FPSS+  +G+  S ST+V  G
Sbjct: 75  FQKKGQASESCNFTGTATLTTTDPSYTGCAFPSSA--SGSSGSGSTTVTPG 122

[Close]
BLAST of Carg04635 vs. ExPASy TrEMBL
Match: A0A6J1EY09 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437228 PE=4 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.8e-90
Identity = 170/173 (98.27%), Postives = 171/173 (98.84%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           SDCSPIQ SGACYDAS+VQ MASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  SDCSPIQPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 174
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 173

[Close]
BLAST of Carg04635 vs. ExPASy TrEMBL
Match: A0A6J1JNE0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486269 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 5.5e-87
Identity = 162/173 (93.64%), Postives = 167/173 (96.53%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           M I+ ALFLLI  I+CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MPINFALFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           +DCSPIQ SGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAA+TSLNPSYGNCRF
Sbjct: 61  ADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMFLI 174
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGR+S RIWVWPIAIGYLFFKIMFLI
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIGYLFFKIMFLI 173

[Close]
BLAST of Carg04635 vs. ExPASy TrEMBL
Match: A0A6J1ES45 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437228 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.0e-85
Identity = 160/165 (96.97%), Postives = 162/165 (98.18%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           SDCSPIQ SGACYDAS+VQ MASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  SDCSPIQPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYL 166
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIG +
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGLM 165

[Close]
BLAST of Carg04635 vs. ExPASy TrEMBL
Match: A0A6J1ERW0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111437228 PE=4 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.0e-85
Identity = 160/163 (98.16%), Postives = 161/163 (98.77%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           SDCSPIQ SGACYDAS+VQ MASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF
Sbjct: 61  SDCSPIQPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIG 164
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIG
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIG 163

[Close]
BLAST of Carg04635 vs. ExPASy TrEMBL
Match: A0A6J1JLF2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486269 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.2e-81
Identity = 152/163 (93.25%), Postives = 157/163 (96.32%), Query Frame = 0 

Query: 1   MEIDLALFLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60
           M I+ ALFLLI  I+CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG
Sbjct: 1   MPINFALFLLIVPILCSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGG 60

Query: 61  SDCSPIQRSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRF 120
           +DCSPIQ SGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAA+TSLNPSYGNCRF
Sbjct: 61  ADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCRF 120

Query: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIG 164
           PSSSLRNGNFSSQSTSVGLGPSEDLSGSGR+S RIWVWPIAIG
Sbjct: 121 PSSSLRNGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIG 163

[Close]
BLAST of Carg04635 vs. TAIR 10
Match: AT3G58100.1 (plasmodesmata callose-binding protein 5 )

HSP 1 Score: 146.4 bits (368), Expect = 2.2e-35
Identity = 71/132 (53.79%), Postives = 94/132 (71.21%), Query Frame = 0 

Query: 26  IKGQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFA 85
           I GQ    ELWCVAKNNA+D+SLQ+A++WACG GG+DC PIQ+ G C D ++VQ MASF 
Sbjct: 31  IGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGPIQQGGPCNDPTDVQKMASFV 90

Query: 86  FNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSSQSTSVGLGPSEDL 145
           FN+Y+LK+G  D++C F N+AA+TSLNPS G C++PSS   N    +  TS+G G + D+
Sbjct: 91  FNNYYLKNGEEDEACNFNNNAALTSLNPSQGTCKYPSSKGANNGRLADDTSMGAGQA-DM 150

Query: 146 SGSGRKSGRIWV 158
           S  GR     W+
Sbjct: 151 SRGGRPISSSWM 161

[Close]
BLAST of Carg04635 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.3e-21
Identity = 50/97 (51.55%), Postives = 65/97 (67.01%), Query Frame = 0 

Query: 28  GQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFAFN 87
           G G  +  WC+AK NA   SLQ ALD+ACG GG+DC  IQ+  ACY+ + +++ ASFAFN
Sbjct: 140 GGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFN 199

Query: 88  DYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSS 125
            Y+ KH    DSC F  +A +TS +PS G+C F SSS
Sbjct: 200 SYYQKH-PGSDSCNFGGAAQLTSTDPSKGSCHFSSSS 235

[Close]
BLAST of Carg04635 vs. TAIR 10
Match: AT4G05430.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 7.5e-20
Identity = 47/99 (47.47%), Postives = 60/99 (60.61%), Query Frame = 0 

Query: 29  QGGATEL----WCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASF 88
           QG  T L    WCVA+  A  A LQ ALDWACG G  DCS I+R G CY+   + + ASF
Sbjct: 12  QGNTTFLEGTTWCVARPGATQAELQRALDWACGIGRVDCSVIERHGDCYEPDTIVSHASF 71

Query: 89  AFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSS 124
           AFN Y+  +G    +C+F  +A  T +NPSYG C + +S
Sbjct: 72  AFNAYYQTNGNNRIACYFGGTATFTKINPSYGKCSYDAS 110

[Close]
BLAST of Carg04635 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.3e-19
Identity = 57/141 (40.43%), Postives = 82/141 (58.16%), Query Frame = 0 

Query: 8   FLLIALIICSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAGGSDCSPIQ 67
           FLL+ L   SAA               +WCVA+ +    +LQ+ALD+AC A G+DC+PIQ
Sbjct: 48  FLLLLLFRSSAA---------------MWCVARFDVTSQALQAALDYAC-AAGADCAPIQ 107

Query: 68  RSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRN 127
            +G C+  + VQ  AS+AFN YF +  M   SC F  ++ I   +PSYG+C +P +S+RN
Sbjct: 108 PNGLCFLPNTVQAHASYAFNSYFQRAAMAPGSCNFAGTSTIAKTDPSYGSCVYP-NSVRN 167

Query: 128 GNFSSQSTSVGLGPSEDLSGS 149
              S+ +T+VG  PS  +  S
Sbjct: 168 AGGSASTTTVGGTPSTTVGNS 171

[Close]
BLAST of Carg04635 vs. TAIR 10
Match: AT1G11820.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.2e-19
Identity = 51/116 (43.97%), Postives = 70/116 (60.34%), Query Frame = 0 

Query: 34  ELWCVAKNNADDASLQSALDWACGAGGSDCSPIQRSGACYDASNVQNMASFAFNDYFLKH 93
           + +C+A +  D  +LQ+ALDWACG G S+CS IQ   +CY  +NV+  ASFAFN Y+ K 
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 94  GMTDDSCFFQNSAAITSLNPSYGNCRFPSSSLRNGNFSS---QSTSVGLGPSEDLS 147
           G    SC F+  A IT+ +PS+G+C FP S  + GN +     ST V  G +   S
Sbjct: 439 GRASGSCDFKGVAMITTTDPSHGSCIFPGSK-KVGNRTQTVVNSTEVAAGEATSRS 493

The following BLAST results are available for this feature:
BLAST of Carg04635 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7022965.13.1e-92100.00PLASMODESMATA CALLOSE-BINDING PROTEIN 5 [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022930875.13.8e-9098.27PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita moschata][more]
XP_022989094.11.1e-8693.64PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita maxima][more]
KAG6589266.12.0e-8698.79PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023529864.17.4e-8692.49PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita pepo subsp. p... [more]
BLAST of Carg04635 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M2K63.1e-3453.79PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5... [more]
O653993.1e-1843.97Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q8VYE51.3e-1639.60Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9C7U51.7e-1634.53Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Q9SD842.2e-1640.54PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2... [more]
BLAST of Carg04635 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1EY091.8e-9098.27PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1JNE05.5e-8793.64PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1ES453.0e-8596.97PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1ERW04.0e-8598.16PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X3 OS=Cucurbita moschata OX... [more]
A0A6J1JLF21.2e-8193.25PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 OS=Cucurbita maxima OX=3... [more]
BLAST of Carg04635 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G58100.12.2e-3553.79plasmodesmata callose-binding protein 5 [more]
AT1G29380.12.3e-2151.55Carbohydrate-binding X8 domain superfamily protein [more]
AT4G05430.17.5e-2047.47Carbohydrate-binding X8 domain superfamily protein [more]
AT4G13600.11.3e-1940.43Carbohydrate-binding X8 domain superfamily protein [more]
AT1G11820.22.2e-1943.97O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 35..120
e-value: 3.3E-40
score: 149.6
IPR012946X8 domainPFAMPF07983X8coord: 35..106
e-value: 1.7E-18
score: 67.1
NoneNo IPR availableGENE3D1.20.58.1040coord: 31..134
e-value: 5.3E-11
score: 44.8
NoneNo IPR availablePANTHERPTHR31044:SF80PLASMODESMATA CALLOSE-BINDING PROTEIN 5coord: 6..152
IPR044788Carbohydrate-binding X8 domain-containing protein, plantPANTHERPTHR31044BETA-1,3 GLUCANASEcoord: 6..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04635-RACarg04635-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma