Carg04440 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04440
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	Carg_Chr04: 20394474 .. 20397600 (-)
RNA-Seq Expression	Carg04440
Synteny	Carg04440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCGAAACTGGGAAACAGAGATCGTTAGAAGAGAGAGGGCTTATCGCGGAAAATCGATTACAGAGACGACAATGGAGGGAGAACCGACTCGAATAATGCTTGCTGTGAATCAGTCAACCATCAAGGGCTATCCACACGCTTCCATAAGCAGCAGTAGGGCCTTCGAATGGACTCTCCAGAAGATCGTCCGTTCCAACACCTCTGGTTTCAAGTTTCTCTTCCTTCATGTTCAAGTACCAGACGAAGACGGTTTGCTCTCCTTCTCTTGATTTCGTTACTCTTTTCTCGTTTTTTTTTTCTTTCCTTTTATGGCCTCTTTGCTTGATGCTGATTGACTTTGTTGAGATGCGATATTGTTCTGTTCGTTGTTTCTTGTTTTGCTGAATCTTGAACTTGGTTAGATGAACTCCGACTTTTCATATGACGAAATATTGGTTGATTGGAAGCATAGAAGGGAATTGTTGTTTTATAAGTGGTGTACAAGGGCATTTAGTCTCGTATTTGATTCAAGAAGTACGGCTTATATGTCTTTCTTGTGATCTATGCCCTAGAATCTTGTTTTCTCCTTGATCTGAACTTGCAAATTAAACGCTAAGAAATATAGAGATCGCACCGATGACTGATTCAATAATTAGCATGTGGATGATGGATTATCTGCTTTAAAATTTCTTGAAGTTTCGAATCTACAAATAGGAAACGAGCTTCCTCTATCTTTTTCTTGGTGTATTTCACTGACGACTTTGTATGGTAGAGCGATCCAAGTTTTCTAACTCTTTTGACTTTTGAACAAAGATTTGAACTGTAGACTAATGCAAATTTTTACATTTCACTCGATCACATCTCTGTGAAATATCTTTTATATAATTATAGAAAACAAAATTCAGTACAACCAGTAAGTATAATACATGTATTCACGTTGCAATATTCAGTTTTAAGCAACTTTATTCCTGGAAGGAAGCGCTCTCCCCTACTTATATAATCATATGTCCTACAAATAAATAACAAGCATCTCAAACAAAAAACTTCCTGATCTAATATTACGGCTTGTTCGAATTTAAGTGTTTAAATAAGTGTTATGTGGAAAAAAAGAAATCTTCTAAGCACTTAGAAAGTTTTTGAAACATGCTCTTAATCTTCAAGTCTTATCACACAACGATTCTAGTCCTTTGCCCCCTGTTTCGTTTCTACATAGTGTTTATTATTTCTCAGTACTACTATTATTAAATAATTGTTAGTGACTCCTTCCTTCATTTCTCTCCTGAGCATGTCTTCTATGTGCCTAAATGTGCCGGAGTTCCTTAGAACTTGAAGACACATAAACAGAGGTCACCTGTTATCCTGGTCTTGTAAGGTTTTGACATGCCCCATTTTCAAACATGCCCAAAATTTTTAAATTAAAAAAAAAATTAAAAGACATGCATCCAAATCACATGTAGTGCACGTTTTTTCTTATAAATATTCTGTACAAAAACAATTTGATGTTTGTGCTTCCCTTAACTCCTATTTGGTTTCTATTTTTTTTGCCATAATTGGATGTTTTTTCTTAGAATGAGTTTGAATAGCTTTTGAGACGCTGGAAAGTACTTTAATCATTTTTAGTTTTTCGTTTGATTATCAAAATAACTTCAAAAATTCTCGAAGCCCTTGTCTTTAAGATAGCACATAATTTTGCTCAAGGTAAATGGCGAATAGACTATTTTCTTGTGTGGTTTTATACATATATATGCACACACATGTAGTTATGTATGTATAAACCATTTACCATCTTCTTGAAAATTATAGATTCATTGGCCATTTCTGAGAAAGATTATCTCCTTTTTTACTGTTTCATAATAGTTCTGAACATAATGTTTACTGGTTTCGTTAGAAGGTACTATACTATACTATGACTCGAGGATGTGGATTATATTTTGTACAGTATTACTATGATGCTATATTTCTTGGAATAGTTTCGATAGTTTGACCGTCTTGATGACTTTTCCCATCTCTTTGATTTTTTCTTCTTTTTTACAAGGGAACTGAGGTGTGGGATGGCAGTGTACGCACCATATTTCAAAATGATACTGATTCCACGTATCACATGTTAGAAAATAACTAGTAACTGAAACAGTACTCTGCTCATTTTATTTCTTGTAGGTTTTGACGACGTAGATAGTATCTTTGCGTCACCTGATGATTTTAAAGACCTGAAGCAAAGGGACAATGCACGAGGGCTTCAACTACTGGAGTTCTTTGTCAATAGATGTCATGAAATTGGGGTAATCCTTGTTTATACGACTTTTTCTGCTGGATTGATCTATCAATGTAAATATGCAAAATTATGATCACACTTTACCTACTGTTGGTACACCAGAAAAATCAAACAATAATAGTCTGTTTAACACCAAAAAGAAGGCAATGTTAGGACAATCTTTTTTGGATAATTAGACAGACTATTTTCTTTCCTGACATAGTTCTATTGCAATTGGTCGTGTTCATTGCTTGTTGCAATACTTTTCTGTAGGTTGCTTGTGAAGCTTGGCTCAAGAAGGGTGATCCCACTGAAGTAATTTGCCACGAGGTGAAACGCGTGCAACCTGATTTTCTAGTTGTGGGAAGCAGGGGAATTGGCCGGTTCAAAAGGTGTGCTTGAGTTGTATTAGCTCTTCGACATTACTGTTTCTAAATATAGGACTCAATTAATCATTTCTTAGCCTGTTTTTACTTAATACCTTGATGGCATTGGAAGCCTAACTGCCTGGAAAATCCGCTTGTCGATGTCTGCAGGGTCTTTGTAGGCACTGTAAGTGAATTCTGTGCAAAACATGCCGAGTGCCCTGTAATTACGGTCAAACGCACGGTGGACGAGACACCTGAAGATCAAGTCGACGACTGACTTTCCAGCCGTCATAACATGTAGTCGTGCCATTTTGATTTCATTTTATCTTGTTGATAAATAAGAAACTATTGGTGAGCGTGGTCGTGTGTATTTAGTGTGCAAAAGTTGCCAATTAGCCATTGATTTGCATTTGTGTTGTTGGTTCATTGTTTGTTCAATTGATTGATGAAGAATTGGGAATTGGTAACAGACTTTTCATGTGAGATATAGCTAAAACCATTCTCTCTCTAGAAAAAAACAGGTCTTTT

mRNA sequence

CATCGAAACTGGGAAACAGAGATCGTTAGAAGAGAGAGGGCTTATCGCGGAAAATCGATTACAGAGACGACAATGGAGGGAGAACCGACTCGAATAATGCTTGCTGTGAATCAGTCAACCATCAAGGGCTATCCACACGCTTCCATAAGCAGCAGTAGGGCCTTCGAATGGACTCTCCAGAAGATCGTCCGTTCCAACACCTCTGGTTTCAAGTTTCTCTTCCTTCATGTTCAAGTACCAGACGAAGACGGTTTTGACGACGTAGATAGTATCTTTGCGTCACCTGATGATTTTAAAGACCTGAAGCAAAGGGACAATGCACGAGGGCTTCAACTACTGGAGTTCTTTGTCAATAGATGTCATGAAATTGGGGTTGCTTGTGAAGCTTGGCTCAAGAAGGGTGATCCCACTGAAGTAATTTGCCACGAGGTGAAACGCGTGCAACCTGATTTTCTAGTTGTGGGAAGCAGGGGAATTGGCCGGTTCAAAAGGGTCTTTGTAGGCACTGTAAGTGAATTCTGTGCAAAACATGCCGAGTGCCCTGTAATTACGGTCAAACGCACGGTGGACGAGACACCTGAAGATCAAGTCGACGACTGACTTTCCAGCCGTCATAACATGTAGTCGTGCCATTTTGATTTCATTTTATCTTGTTGATAAATAAGAAACTATTGGTGAGCGTGGTCGTGTGTATTTAGTGTGCAAAAGTTGCCAATTAGCCATTGATTTGCATTTGTGTTGTTGGTTCATTGTTTGTTCAATTGATTGATGAAGAATTGGGAATTGGTAACAGACTTTTCATGTGAGATATAGCTAAAACCATTCTCTCTCTAGAAAAAAACAGGTCTTTT

Coding sequence (CDS)

ATGGAGGGAGAACCGACTCGAATAATGCTTGCTGTGAATCAGTCAACCATCAAGGGCTATCCACACGCTTCCATAAGCAGCAGTAGGGCCTTCGAATGGACTCTCCAGAAGATCGTCCGTTCCAACACCTCTGGTTTCAAGTTTCTCTTCCTTCATGTTCAAGTACCAGACGAAGACGGTTTTGACGACGTAGATAGTATCTTTGCGTCACCTGATGATTTTAAAGACCTGAAGCAAAGGGACAATGCACGAGGGCTTCAACTACTGGAGTTCTTTGTCAATAGATGTCATGAAATTGGGGTTGCTTGTGAAGCTTGGCTCAAGAAGGGTGATCCCACTGAAGTAATTTGCCACGAGGTGAAACGCGTGCAACCTGATTTTCTAGTTGTGGGAAGCAGGGGAATTGGCCGGTTCAAAAGGGTCTTTGTAGGCACTGTAAGTGAATTCTGTGCAAAACATGCCGAGTGCCCTGTAATTACGGTCAAACGCACGGTGGACGAGACACCTGAAGATCAAGTCGACGACTGA

Protein sequence

MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDGFDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD

Homology

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BLAST of Carg04440 vs. NCBI nr
Match: XP_022960327.1 (universal stress protein A-like protein [Cucurbita moschata] >KAG6602433.1 Universal stress protein A-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033113.1 Universal stress protein A-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362.8 bits (930), Expect = 1.6e-96
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG
Sbjct: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV
Sbjct: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD
Sbjct: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 175

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BLAST of Carg04440 vs. NCBI nr
Match: XP_023536546.1 (universal stress protein A-like protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.1 bits (915), Expect = 8.9e-95
Identity = 173/175 (98.86%), Postives = 174/175 (99.43%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG
Sbjct: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVIC EV
Sbjct: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICLEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQV+D
Sbjct: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVED 175

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BLAST of Carg04440 vs. NCBI nr
Match: XP_022989906.1 (universal stress protein A-like protein [Cucurbita maxima])

HSP 1 Score: 356.7 bits (914), Expect = 1.2e-94
Identity = 173/175 (98.86%), Postives = 173/175 (98.86%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG
Sbjct: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVIC EV
Sbjct: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICLEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPED VDD
Sbjct: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDHVDD 175

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BLAST of Carg04440 vs. NCBI nr
Match: XP_038890577.1 (universal stress protein A-like protein [Benincasa hispida])

HSP 1 Score: 326.6 bits (836), Expect = 1.3e-85
Identity = 156/175 (89.14%), Postives = 163/175 (93.14%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGE TRIM+AVNQSTIKGYPH SISS RAFEWTLQKIVRSNTSGFKFLFLHV VPDEDG
Sbjct: 1   MEGEQTRIMVAVNQSTIKGYPHPSISSKRAFEWTLQKIVRSNTSGFKFLFLHVLVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFK+LKQRDNARGL LLE+FV RCHEIGVACE+WLKKGDPTEVIC E 
Sbjct: 61  FDDVDSIFASPDDFKELKQRDNARGLHLLEYFVTRCHEIGVACESWLKKGDPTEVICLEA 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRVQPDFLVVGSRG+G FK+VFVGTVSEFCAKHAECPVIT+KR  DETPED VDD
Sbjct: 121 KRVQPDFLVVGSRGLGPFKKVFVGTVSEFCAKHAECPVITIKRREDETPEDPVDD 175

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BLAST of Carg04440 vs. NCBI nr
Match: XP_008458777.1 (PREDICTED: universal stress protein A-like protein [Cucumis melo])

HSP 1 Score: 325.9 bits (834), Expect = 2.2e-85
Identity = 156/175 (89.14%), Postives = 164/175 (93.71%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           ME EPTRIMLAVNQSTIKGYPH SISS RAFEWTLQKIVRSNTSGFKFLFLHV VPDEDG
Sbjct: 1   MEEEPTRIMLAVNQSTIKGYPHPSISSKRAFEWTLQKIVRSNTSGFKFLFLHVLVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FD+VDSIFASPDDFK+LK+RDNARGL LLE+FVNRCHEIGVACEAWLKKGDPTEVIC EV
Sbjct: 61  FDEVDSIFASPDDFKELKKRDNARGLHLLEYFVNRCHEIGVACEAWLKKGDPTEVICLEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRV PDFLVVGSRG+G FK+VFVGTVSEFCAKHAECPVIT+KR  DETP+D VDD
Sbjct: 121 KRVLPDFLVVGSRGLGPFKKVFVGTVSEFCAKHAECPVITIKRREDETPDDPVDD 175

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BLAST of Carg04440 vs. ExPASy Swiss-Prot
Match: Q8LGG8 (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 282.7 bits (722), Expect = 2.8e-75
Identity = 129/175 (73.71%), Postives = 150/175 (85.71%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           M  EPT++M+AVN STIK YP+ SIS  RAFEWTL+KIVRSNTS FK L LHVQV DEDG
Sbjct: 1   MGSEPTKVMVAVNASTIKDYPNPSISCKRAFEWTLEKIVRSNTSDFKILLLHVQVVDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSI+ASP+DF+D++Q + A+GL LLEFFVN+CHEIGV CEAW+K GDP +VIC EV
Sbjct: 61  FDDVDSIYASPEDFRDMRQSNKAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRV+PDFLVVGSRG+GRF++VFVGTVS FC KHAECPV+T+KR  DETP D  DD
Sbjct: 121 KRVRPDFLVVGSRGLGRFQKVFVGTVSAFCVKHAECPVMTIKRNADETPSDPADD 175

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BLAST of Carg04440 vs. ExPASy Swiss-Prot
Match: P74148 (Universal stress protein Sll1388 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1388 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.5e-07
Identity = 28/88 (31.82%), Postives = 44/88 (50.00%), Query Frame = 0


Query: 75  KDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQPDFLVVGSRG 134
           K+  +       + L+  V +  E GVACE  +K G+P   I    K    D +V+G RG
Sbjct: 66  KEHLEEQQTEAREWLQSIVQQVQEDGVACEWDVKVGEPGRWIRDMAKNWDADLVVLGRRG 125

Query: 135 IGRFKRVFVGTVSEFCAKHAECPVITVK 163
           +     VF+G+VS +   H +C V+ V+
Sbjct: 126 LKGLAEVFLGSVSSYVIHHVQCSVLIVQ 153

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BLAST of Carg04440 vs. ExPASy Swiss-Prot
Match: P42297 (Universal stress protein YxiE OS=Bacillus subtilis (strain 168) OX=224308 GN=yxiE PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 5.5e-07
Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 0


Query: 71  PDDFKD-LKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQPDFLV 130
           P+ F D ++      GL++LE    +  E GV  E     G+P   I +  K      +V
Sbjct: 56  PEHFIDEIRNEVKKEGLKILENAKEKAAEKGVQAETIYANGEPAHEILNHAKEKGVSLIV 115

Query: 131 VGSRGIGRFKRVFVGTVSEFCAKHAECPVITVK 163
           VGSRGI   K + +G+VS   ++ + CPV+ V+
Sbjct: 116 VGSRGISGLKEMMLGSVSHKVSQLSTCPVLIVR 148

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BLAST of Carg04440 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 8.8e-05
Identity = 31/94 (32.98%), Postives = 48/94 (51.06%), Query Frame = 0


Query: 72  DDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQPDFLVVG 131
           D F   K  D A+ L+   F     H+I +     +K  D  E +C E +R+    +++G
Sbjct: 120 DAFTSSKVADLAKPLKEAGF----PHKIHI-----VKDHDMRERLCLETERLNLSAVIMG 179

Query: 132 SRGIGRFKRVF---VGTVSEFCAKHAECPVITVK 163
           SRG G  KR     +G+VS++C  H  CPV+ V+
Sbjct: 180 SRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVR 204

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BLAST of Carg04440 vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 8.8e-05
Identity = 24/83 (28.92%), Postives = 42/83 (50.60%), Query Frame = 0


Query: 85  GLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQPDFLVVGSRGIGRFKRVFVG 144
           G + L+       E GV     + +G P   I    ++ + D +V+G+ G    +R+ +G
Sbjct: 88  GQEALKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLIVMGTTGKTGLERILLG 147

Query: 145 TVSEFCAKHAECPVITVKRTVDE 168
           +V+E   K+A CPV+ VK+   E
Sbjct: 148 SVAERVIKNAHCPVLVVKKPKKE 170

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BLAST of Carg04440 vs. ExPASy TrEMBL
Match: A0A6J1H737 (universal stress protein A-like protein OS=Cucurbita moschata OX=3662 GN=LOC111461085 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 7.8e-97
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG
Sbjct: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV
Sbjct: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD
Sbjct: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 175

[Close]

BLAST of Carg04440 vs. ExPASy TrEMBL
Match: A0A6J1JLH1 (universal stress protein A-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486957 PE=4 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 5.6e-95
Identity = 173/175 (98.86%), Postives = 173/175 (98.86%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG
Sbjct: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVIC EV
Sbjct: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICLEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPED VDD
Sbjct: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDHVDD 175

[Close]

BLAST of Carg04440 vs. ExPASy TrEMBL
Match: A0A1S3C9W4 (universal stress protein A-like protein OS=Cucumis melo OX=3656 GN=LOC103498079 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 1.1e-85
Identity = 156/175 (89.14%), Postives = 164/175 (93.71%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           ME EPTRIMLAVNQSTIKGYPH SISS RAFEWTLQKIVRSNTSGFKFLFLHV VPDEDG
Sbjct: 1   MEEEPTRIMLAVNQSTIKGYPHPSISSKRAFEWTLQKIVRSNTSGFKFLFLHVLVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FD+VDSIFASPDDFK+LK+RDNARGL LLE+FVNRCHEIGVACEAWLKKGDPTEVIC EV
Sbjct: 61  FDEVDSIFASPDDFKELKKRDNARGLHLLEYFVNRCHEIGVACEAWLKKGDPTEVICLEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRV PDFLVVGSRG+G FK+VFVGTVSEFCAKHAECPVIT+KR  DETP+D VDD
Sbjct: 121 KRVLPDFLVVGSRGLGPFKKVFVGTVSEFCAKHAECPVITIKRREDETPDDPVDD 175

[Close]

BLAST of Carg04440 vs. ExPASy TrEMBL
Match: A0A6J1BX18 (universal stress protein A-like protein OS=Momordica charantia OX=3673 GN=LOC111006432 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 5.3e-85
Identity = 154/175 (88.00%), Postives = 161/175 (92.00%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           MEGEPTRIMLAVNQSTIKGYPHASISS+RAF+WTL KIVRSNTSGFK LFLHVQ+PDED 
Sbjct: 1   MEGEPTRIMLAVNQSTIKGYPHASISSTRAFQWTLHKIVRSNTSGFKLLFLHVQIPDEDA 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSIFASPDDFKDLK R+ ARGL LLEFFVNRCHEIGV CEAWLKKGDPTEVIC E 
Sbjct: 61  FDDVDSIFASPDDFKDLKHRERARGLLLLEFFVNRCHEIGVGCEAWLKKGDPTEVICLEA 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRV+PDFLVVGSRG+G FKRVFVGTVSEFC KHAECPVIT+KR  DETPED VDD
Sbjct: 121 KRVRPDFLVVGSRGLGPFKRVFVGTVSEFCVKHAECPVITIKRGEDETPEDPVDD 175

[Close]

BLAST of Carg04440 vs. ExPASy TrEMBL
Match: A0A0A0KP43 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524760 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.2e-83
Identity = 152/175 (86.86%), Postives = 162/175 (92.57%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           ME  PTRIMLAVNQSTIKGYPH SISS RAFEWTLQKIVRSNTSGF+FLFLHV VPDEDG
Sbjct: 1   MEEGPTRIMLAVNQSTIKGYPHPSISSKRAFEWTLQKIVRSNTSGFRFLFLHVLVPDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FD+VDSIFASPDDFK+LK+RDNARGL LLE+FV RCHEIGVA EAWLKKGDPTEVIC EV
Sbjct: 61  FDEVDSIFASPDDFKELKKRDNARGLHLLEYFVTRCHEIGVASEAWLKKGDPTEVICLEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KR+QPDFLVVGSRG+G FK+VFVGTVSEFCAKHAECPVIT+KR  DETP+D VDD
Sbjct: 121 KRIQPDFLVVGSRGLGPFKKVFVGTVSEFCAKHAECPVITIKRREDETPDDPVDD 175

[Close]

BLAST of Carg04440 vs. TAIR 10
Match: AT5G14680.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 299.3 bits (765), Expect = 2.0e-81
Identity = 132/175 (75.43%), Postives = 160/175 (91.43%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           ME EPTR+M+AVN+ST+KGYPHASISS +AFEWTL+KIVRSNTSGFK L LHVQV DEDG
Sbjct: 1   MESEPTRVMVAVNESTLKGYPHASISSKKAFEWTLKKIVRSNTSGFKLLLLHVQVQDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDD+DSI+ASPDDF+ +++R+ A+GL LLEFFV +CH+IGV CEAW++KGDPTE+ICHEV
Sbjct: 61  FDDMDSIYASPDDFRQMRERNKAKGLHLLEFFVKKCHDIGVGCEAWIRKGDPTELICHEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           +RV+PDFLVVGSRG+G F++VFVGTVSEFC KHAECPVIT+KRT +E+P+D  DD
Sbjct: 121 RRVRPDFLVVGSRGLGPFQKVFVGTVSEFCVKHAECPVITIKRTAEESPQDPADD 175

[Close]

BLAST of Carg04440 vs. TAIR 10
Match: AT3G01520.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 2.0e-76
Identity = 129/175 (73.71%), Postives = 150/175 (85.71%), Query Frame = 0


Query: 1   MEGEPTRIMLAVNQSTIKGYPHASISSSRAFEWTLQKIVRSNTSGFKFLFLHVQVPDEDG 60
           M  EPT++M+AVN STIK YP+ SIS  RAFEWTL+KIVRSNTS FK L LHVQV DEDG
Sbjct: 1   MGSEPTKVMVAVNASTIKDYPNPSISCKRAFEWTLEKIVRSNTSDFKILLLHVQVVDEDG 60

Query: 61  FDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEV 120
           FDDVDSI+ASP+DF+D++Q + A+GL LLEFFVN+CHEIGV CEAW+K GDP +VIC EV
Sbjct: 61  FDDVDSIYASPEDFRDMRQSNKAKGLHLLEFFVNKCHEIGVGCEAWIKTGDPKDVICQEV 120

Query: 121 KRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVKRTVDETPEDQVDD 176
           KRV+PDFLVVGSRG+GRF++VFVGTVS FC KHAECPV+T+KR  DETP D  DD
Sbjct: 121 KRVRPDFLVVGSRGLGRFQKVFVGTVSAFCVKHAECPVMTIKRNADETPSDPADD 175

[Close]

BLAST of Carg04440 vs. TAIR 10
Match: AT3G11930.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.3e-16
Identity = 35/97 (36.08%), Postives = 58/97 (59.79%), Query Frame = 0


Query: 66  SIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQP 125
           +++AS    + +K+        LL   +  C    +  E  + +G+  E+IC  V+++  
Sbjct: 96  TVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICEAVEKMHV 155

Query: 126 DFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVK 163
           D LVVGSRG+G+ KR F+G+VS++CA HA CP++ VK
Sbjct: 156 DLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK 192

[Close]

BLAST of Carg04440 vs. TAIR 10
Match: AT3G11930.4 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.3e-16
Identity = 35/97 (36.08%), Postives = 58/97 (59.79%), Query Frame = 0


Query: 66  SIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACEAWLKKGDPTEVICHEVKRVQP 125
           +++AS    + +K+        LL   +  C    +  E  + +G+  E+IC  V+++  
Sbjct: 98  AVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTETLVLEGEAKEMICEAVEKMHV 157

Query: 126 DFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVK 163
           D LVVGSRG+G+ KR F+G+VS++CA HA CP++ VK
Sbjct: 158 DLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK 194

[Close]

BLAST of Carg04440 vs. TAIR 10
Match: AT3G11930.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 3.0e-16
Identity = 40/118 (33.90%), Postives = 64/118 (54.24%), Query Frame = 0


Query: 51  LHVQVPDED------GFDDVDSIFASPDDFKDLKQRDNARGLQLLEFFVNRCHEIGVACE 110
           +HVQ P         G     +++AS    + +K+        LL   +  C    +  E
Sbjct: 76  IHVQSPFNHFAAFPAGPGGATAVYASSSMIESVKKAQQETSAALLSRALQMCRAKQIRTE 135

Query: 111 AWLKKGDPTEVICHEVKRVQPDFLVVGSRGIGRFKRVFVGTVSEFCAKHAECPVITVK 163
             + +G+  E+IC  V+++  D LVVGSRG+G+ KR F+G+VS++CA HA CP++ VK
Sbjct: 136 TLVLEGEAKEMICEAVEKMHVDLLVVGSRGLGKIKRAFLGSVSDYCAHHANCPILIVK 193

The following BLAST results are available for this feature:

BLAST of Carg04440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022960327.1	1.6e-96	100.00	universal stress protein A-like protein [Cucurbita moschata] >KAG6602433.1 Unive...	[more]
XP_023536546.1	8.9e-95	98.86	universal stress protein A-like protein [Cucurbita pepo subsp. pepo]	[more]
XP_022989906.1	1.2e-94	98.86	universal stress protein A-like protein [Cucurbita maxima]	[more]
XP_038890577.1	1.3e-85	89.14	universal stress protein A-like protein [Benincasa hispida]	[more]
XP_008458777.1	2.2e-85	89.14	PREDICTED: universal stress protein A-like protein [Cucumis melo]	[more]

BLAST of Carg04440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LGG8	2.8e-75	73.71	Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g0...	[more]
P74148	2.5e-07	31.82	Universal stress protein Sll1388 OS=Synechocystis sp. (strain PCC 6803 / Kazusa)...	[more]
P42297	5.5e-07	32.26	Universal stress protein YxiE OS=Bacillus subtilis (strain 168) OX=224308 GN=yxi...	[more]
Q8L4N1	8.8e-05	32.98	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
Q57951	8.8e-05	28.92	Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]

BLAST of Carg04440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H737	7.8e-97	100.00	universal stress protein A-like protein OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1JLH1	5.6e-95	98.86	universal stress protein A-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486...	[more]
A0A1S3C9W4	1.1e-85	89.14	universal stress protein A-like protein OS=Cucumis melo OX=3656 GN=LOC103498079 ...	[more]
A0A6J1BX18	5.3e-85	88.00	universal stress protein A-like protein OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KP43	2.2e-83	86.86	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G524760 PE=4 SV...	[more]

BLAST of Carg04440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14680.1	2.0e-81	75.43	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G01520.1	2.0e-76	73.71	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.1	2.3e-16	36.08	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.4	2.3e-16	36.08	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G11930.2	3.0e-16	33.90	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 140..162 score: 32.0 coord: 122..134 score: 36.31
IPR006016	UspA	PFAM	PF00582	Usp	coord: 24..162 e-value: 1.7E-18 score: 67.5
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 2..175 e-value: 3.3E-69 score: 234.6
IPR044187	Universal stress protein A-like protein, plant	PANTHER	PTHR47710	ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN	coord: 1..175
None	No IPR available	CDD	cd00293	USP_Like	coord: 7..161 e-value: 7.86278E-23 score: 86.2698
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 6..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04440-RA	Carg04440-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016208	AMP binding