Carg04438 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04438
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncinnamoyl-CoA reductase-like SNL6
LocationCarg_Chr04: 20404527 .. 20406727 (-)
RNA-Seq ExpressionCarg04438
SyntenyCarg04438
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCCTCGCTATCTCACTCTCTCTGTCCTCTAGATTTTCAGTGTAATCGGCGAAATGGGTGTTTTATTTCGTTCCGAGGAAACCTTGGTATCGGAGTTGGAGCAGCTCCGCCGCAAGCTCGTTGCCTGCGGCGGCATCCAGAGGAGGAAGGACGGCGAAGAGTTCAAAAGTCTCAAGTCGAAGCTGATTGACGATGCAGAGGAGAAAGTCGTGTGTGTAACTAGCGGCGTTTCGTTTCTTGGTTCCGCCATCGTTAACGAGCTCTTAGTTCATGGATACTCTGTTCGAGTCATTGTTGATAATCCTGGTGAGTGATTGAAGCTCTTTTGAGTCTTCGATTTGAATTTAATTTCGTGTATCTTGTAGCGTAATCGATCAATTTCAATTGGTTTATGGTTGATCGGAGTAGTTTTTTTGGATTAATAGACTAATGGAAACCCTAAATGTCAGAGGATGTGAACAAATTGGACGAGACGACGAGCGGCCGCCCTAATGTGACGGTAGTTGTGGCGAAGCTTACAGATGTAAATAGCTTAGTGGAAGCCTTCAATGGCTGCCGTGGCGTTTTTCACACCTCTTCTTCCATTGATCCTGCTGGTCTTTCTGGTTATACGGTGAGGGTTTTTTGCTTTACTTTTCTTGGAAAGAAATTTGCTGCTATTTGATGAATGAACCATCCCTCCCCGTCGTCCTCACATCTGATAAGATCGTTTTGAATCTGTTTTCTGCATTGAGAGCTACGATTGCGTTTTATACTTCAAGAACTATAGATACAAACTTCAAATTCAGCCTAATATTAGAATACATTTGTTTGATGAAGAAATTAAAACAACATTGAATGATGCAAACACTTATTCTTAGATATAATGCGATGAAAGTGATAGTTTAAGGTGAAGTTAAGTTAAGTTTAGAGATTAAAATGGGTTAATTGTAACTGTCAAAATCTTGGGTAGATATTGTCCTCTTTAGATTTTCCATTTCAGGTTTACTGGTGTTTCGTTCTCCTCCTCAATTGATATGGGATCTCACAATCCACCCCTTTCGGGACCCAGCATCCTCGCTGGCACACCGTCCAGTGTCTACCCCATGTTGTTCCCCTACGCTAGGCCCTAAAGAGGGGTGGATTGTGAGATCCCACATCGATTATTGAAGAGAACAAAACATTCTTTAGAAGGGTGTGGAAACCTGTCCCTAATAGAAGTGTTTTAAAAACCTTGAGGGAAGGAGTCAAAGAATACCATAGTTACATTATTTCTCCGGAAACTATTGAAAGTTTTCTAAAGCTATTTGGGGATTGGACTTTTGTACACAGAAAGTAATGAGCGAAGTGGAAATGAGGAGCACTGAGAATGTGATGGAGGCGTGCGCAAGAACAGAAACAGTACGAAATTGTGTTCTTACATCTTCTCTCCTTGCTTGCATCTGGCGGGACCAACCTGAGAGTACTGGAACCTCACGAGTTGTCGACCATGGTTGCTGGAGCAGTTTCCAGCTCTGCCAAGATAAAAAGGTCGCTCCCTTTCTTTTTCCTCGGTCCACCATTATGAATTTAATTACTGTAGAAAGCTTTAGAATTGAGATTAGATGGAGCTCTAACAAACAAGTTGATGATTATGCAGCTATGGTTTGCATTGGGGAAGCTAAAATCTGAAAAAGTAGCATGGAAAATAGCACAAGAAAGTGGCCTAAAACTAGCCACCATCTGTTCACCTCTCATAACTGGCTCCAACTTCCATTCCAGAAATTCAACTCCAACAATGGCTTATCTCAAAGGTAAAACTTCAAGAACAAACAACCCAGCAGAAGCTTTCATTGATTCCATTTTCCATTAACAAGAACACATTATGCATATCAAACTCAAAGTCTTCATTGATATCAATGCAGGAGCTCGAGAGATGTACAAAAATGGTCTACTGGCAACAGTGGACGTGACCAAATTGGCCAAAGCACATGTATGTATATATGAAGAATTAAAGGAAAATGGTTCCGGCAGATACATTTGCTTCGACCGAGTTATCTCGACGGTCGACGAGGCGACAAAGTTGGCAGCAGAGATCAGAGTCCCTGTCGGGACTATAGTCGAAACCACCCTTCCAATCGACTCGCCAATACCGTTTCAATTGTCGAACAAGAAGGTATCCGATCTAATGTCTAGAAGTAACGTTGGACGTAGCTGTTTGAATGAAAACAGATTTTATTGA

mRNA sequence

TTCCCTCGCTATCTCACTCTCTCTGTCCTCTAGATTTTCAGTGTAATCGGCGAAATGGGTGTTTTATTTCGTTCCGAGGAAACCTTGGTATCGGAGTTGGAGCAGCTCCGCCGCAAGCTCGTTGCCTGCGGCGGCATCCAGAGGAGGAAGGACGGCGAAGAGTTCAAAAGTCTCAAGTCGAAGCTGATTGACGATGCAGAGGAGAAAGTCGTGTGTGTAACTAGCGGCGTTTCGTTTCTTGGTTCCGCCATCGTTAACGAGCTCTTAGTTCATGGATACTCTGTTCGAGTCATTGTTGATAATCCTGAGGATGTGAACAAATTGGACGAGACGACGAGCGGCCGCCCTAATGTGACGGTAGTTGTGGCGAAGCTTACAGATGTAAATAGCTTAGTGGAAGCCTTCAATGGCTGCCGTGGCGTTTTTCACACCTCTTCTTCCATTGATCCTGCTGGTCTTTCTGGTTATACGGCGTGCGCAAGAACAGAAACAGTACGAAATTGTGTTCTTACATCTTCTCTCCTTGCTTGCATCTGGCGGGACCAACCTGAGAGTACTGGAACCTCACGAGTTGTCGACCATGGTTGCTGGAGCAGTTTCCAGCTCTGCCAAGATAAAAAGCTATGGTTTGCATTGGGGAAGCTAAAATCTGAAAAAGTAGCATGGAAAATAGCACAAGAAAGTGGCCTAAAACTAGCCACCATCTGTTCACCTCTCATAACTGGCTCCAACTTCCATTCCAGAAATTCAACTCCAACAATGGCTTATCTCAAAGGAGCTCGAGAGATGTACAAAAATGGTCTACTGGCAACAGTGGACGTGACCAAATTGGCCAAAGCACATGTATGTATATATGAAGAATTAAAGGAAAATGGTTCCGGCAGATACATTTGCTTCGACCGAGTTATCTCGACGGTCGACGAGGCGACAAAGTTGGCAGCAGAGATCAGAGTCCCTGTCGGGACTATAGTCGAAACCACCCTTCCAATCGACTCGCCAATACCGTTTCAATTGTCGAACAAGAAGGTATCCGATCTAATGTCTAGAAGTAACGTTGGACGTAGCTGTTTGAATGAAAACAGATTTTATTGA

Coding sequence (CDS)

ATGGGTGTTTTATTTCGTTCCGAGGAAACCTTGGTATCGGAGTTGGAGCAGCTCCGCCGCAAGCTCGTTGCCTGCGGCGGCATCCAGAGGAGGAAGGACGGCGAAGAGTTCAAAAGTCTCAAGTCGAAGCTGATTGACGATGCAGAGGAGAAAGTCGTGTGTGTAACTAGCGGCGTTTCGTTTCTTGGTTCCGCCATCGTTAACGAGCTCTTAGTTCATGGATACTCTGTTCGAGTCATTGTTGATAATCCTGAGGATGTGAACAAATTGGACGAGACGACGAGCGGCCGCCCTAATGTGACGGTAGTTGTGGCGAAGCTTACAGATGTAAATAGCTTAGTGGAAGCCTTCAATGGCTGCCGTGGCGTTTTTCACACCTCTTCTTCCATTGATCCTGCTGGTCTTTCTGGTTATACGGCGTGCGCAAGAACAGAAACAGTACGAAATTGTGTTCTTACATCTTCTCTCCTTGCTTGCATCTGGCGGGACCAACCTGAGAGTACTGGAACCTCACGAGTTGTCGACCATGGTTGCTGGAGCAGTTTCCAGCTCTGCCAAGATAAAAAGCTATGGTTTGCATTGGGGAAGCTAAAATCTGAAAAAGTAGCATGGAAAATAGCACAAGAAAGTGGCCTAAAACTAGCCACCATCTGTTCACCTCTCATAACTGGCTCCAACTTCCATTCCAGAAATTCAACTCCAACAATGGCTTATCTCAAAGGAGCTCGAGAGATGTACAAAAATGGTCTACTGGCAACAGTGGACGTGACCAAATTGGCCAAAGCACATGTATGTATATATGAAGAATTAAAGGAAAATGGTTCCGGCAGATACATTTGCTTCGACCGAGTTATCTCGACGGTCGACGAGGCGACAAAGTTGGCAGCAGAGATCAGAGTCCCTGTCGGGACTATAGTCGAAACCACCCTTCCAATCGACTCGCCAATACCGTTTCAATTGTCGAACAAGAAGGTATCCGATCTAATGTCTAGAAGTAACGTTGGACGTAGCTGTTTGAATGAAAACAGATTTTATTGA

Protein sequence

MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGCRGVFHTSSSIDPAGLSGYTACARTETVRNCVLTSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRVISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRFY
Homology
BLAST of Carg04438 vs. NCBI nr
Match: KAG7033115.1 (Cinnamoyl-CoA reductase-like SNL6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 685.3 bits (1767), Expect = 2.8e-193
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYTACARTETVRNCVLTSSLLACIWRDQPESTGTSRVVDHGCWS 180
           RGVFHTSSSIDPAGLSGYTACARTETVRNCVLTSSLLACIWRDQPESTGTSRVVDHGCWS
Sbjct: 121 RGVFHTSSSIDPAGLSGYTACARTETVRNCVLTSSLLACIWRDQPESTGTSRVVDHGCWS 180

Query: 181 SFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITGSNFHSRNSTPTMAYLK 240
           SFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITGSNFHSRNSTPTMAYLK
Sbjct: 181 SFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITGSNFHSRNSTPTMAYLK 240

Query: 241 GAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRVISTVDEATKLAAEIRV 300
           GAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRVISTVDEATKLAAEIRV
Sbjct: 241 GAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRVISTVDEATKLAAEIRV 300

Query: 301 PVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRFY 346
           PVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRFY
Sbjct: 301 PVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRFY 345

BLAST of Carg04438 vs. NCBI nr
Match: KAG6602435.1 (Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.9 bits (1740), Expect = 3.8e-190
Identity = 345/361 (95.57%), Postives = 345/361 (95.57%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAGLSGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240
           PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG
Sbjct: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240

Query: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300
           SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV
Sbjct: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300

Query: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 346
           ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF
Sbjct: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 360

BLAST of Carg04438 vs. NCBI nr
Match: XP_022953600.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita moschata])

HSP 1 Score: 673.7 bits (1737), Expect = 8.4e-190
Identity = 344/361 (95.29%), Postives = 345/361 (95.57%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAGLSGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240
           PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG
Sbjct: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240

Query: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300
           SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV
Sbjct: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300

Query: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 346
           ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSN+GRSCLNENRF
Sbjct: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNLGRSCLNENRF 360

BLAST of Carg04438 vs. NCBI nr
Match: XP_022990996.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita maxima])

HSP 1 Score: 667.5 bits (1721), Expect = 6.0e-188
Identity = 340/361 (94.18%), Postives = 343/361 (95.01%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEF+SLKSKLIDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFRSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAGLSGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240
           PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG
Sbjct: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240

Query: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300
           SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVC+YEELKENGSGRYICFDRV
Sbjct: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCVYEELKENGSGRYICFDRV 300

Query: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 346
           ISTVDEATKLA EI+VPV TIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF
Sbjct: 301 ISTVDEATKLAVEIKVPVETIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 360

BLAST of Carg04438 vs. NCBI nr
Match: XP_023528175.1 (cinnamoyl-CoA reductase-like SNL6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 661.4 bits (1705), Expect = 4.3e-186
Identity = 338/361 (93.63%), Postives = 341/361 (94.46%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFR EETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAE+KVVCVTSGVS
Sbjct: 1   MGVLFRPEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEQKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETT GRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTCGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAGLSGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240
           PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG
Sbjct: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240

Query: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300
           SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVC+YEELKE GSGRYICFDRV
Sbjct: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCVYEELKETGSGRYICFDRV 300

Query: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 346
           ISTVDEATKLAAEIRVPVGTIVETTLPI+SPI FQLSNKKVSDLMSRSNVGRSCLNENRF
Sbjct: 301 ISTVDEATKLAAEIRVPVGTIVETTLPINSPILFQLSNKKVSDLMSRSNVGRSCLNENRF 360

BLAST of Carg04438 vs. ExPASy Swiss-Prot
Match: Q0JKZ0 (Cinnamoyl-CoA reductase-like SNL6 OS=Oryza sativa subsp. japonica OX=39947 GN=SNL6 PE=3 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.9e-70
Identity = 152/364 (41.76%), Postives = 208/364 (57.14%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLV----------ACGGIQRRKDGEEFKSLKSKLIDDAEE 60
           MGVL RS +++ +E+E++R  L+          A  G +      + K         A  
Sbjct: 1   MGVL-RSTQSMQAEVEEMRAALLHGHGGGAAAAAAAGWRPSAGDADVKRTAGGDGGAAGP 60

Query: 61  KVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNKLDET----TSGRPNVTVVVAK 120
           + VCVT G+SF+G A+V+ LL HGY+VR+ ++  ED++KL E       GR  V  V+A 
Sbjct: 61  RTVCVTGGISFVGFAVVDRLLRHGYTVRLALETQEDLDKLREMEMFGEDGRDGVWTVMAN 120

Query: 121 LTDVNSLVEAFNGCRGVFHTSSSIDPAGLSGYT----------------ACARTETVRNC 180
           +TD  SL  AF+GC GVFHTS+ +DP G+SGYT                AC RTE+VR C
Sbjct: 121 VTDPESLHRAFDGCAGVFHTSAFVDPGGMSGYTKHMASLEAKAAEQVIEACVRTESVRKC 180

Query: 181 VLTSSLLACIWR-DQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQE 240
           V TSSLLAC+WR + P       ++D  CWS    C+D KLWFALGK  +EK AW+ A+ 
Sbjct: 181 VFTSSLLACVWRQNYPHDRRFPTIIDENCWSDESFCRDNKLWFALGKTAAEKTAWRAARG 240

Query: 241 SGLKLATICSPLITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEE 300
             LKL T+C  L+TG  F  RNST ++AYLKGAR M  +GLLAT  V  +A+AHV +YE 
Sbjct: 241 RDLKLVTVCPALVTGPGFRRRNSTASIAYLKGARAMLADGLLATASVETVAEAHVRVYEA 300

Query: 301 LKEN-GSGRYICFDRVISTVDEATKLAAEIRVP--VGTIVETTLPIDSPIPFQLSNKKVS 331
           + +N   GRYIC+D V+   +E  +L  ++ +P             D P  F L  +K++
Sbjct: 301 MGDNTAGGRYICYDHVVKRPEEFAELERQLGIPRRAAAAAAAQDSGDRPARFDLCRQKLA 360

BLAST of Carg04438 vs. ExPASy Swiss-Prot
Match: A0A059TC02 (Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-33
Identity = 105/309 (33.98%), Postives = 150/309 (48.54%), Query Frame = 0

Query: 51  KVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNK--LDETTSGRPNVTVVVAKLT 110
           +VVCVT    F+ S +V  LL  GY+VR  V NP+D     L E    +  +T+  A L 
Sbjct: 7   QVVCVTGAGGFIASWLVKILLEKGYTVRGTVRNPDDPKNGHLRELEGAKERLTLCKADLL 66

Query: 111 DVNSLVEAFNGCRGVFHTSS--------SIDPA--GLSGYTACARTETVRNCVLTSSLLA 170
           D  SL EA NGC GVFHT+S         ++PA  G       A    VR  V TSS + 
Sbjct: 67  DYQSLREAINGCDGVFHTASPVTDDPEQMVEPAVIGTKNVINAAAEANVRRVVFTSS-IG 126

Query: 171 CIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATIC 230
            ++ D      T  VVD  CWS    C++ K W+  GK+ +E+ AW+ A+E G+ L  I 
Sbjct: 127 AVYMDPNRDPET--VVDETCWSDPDFCKNTKNWYCYGKMVAEQAAWEEAKEKGVDLVVIN 186

Query: 231 SPLITGSNFHSRNSTPT---MAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGS 290
             L+ G    +  +      + YL G+ + Y N + A VDV  +A AH+ +YE      S
Sbjct: 187 PVLVQGPLLQTTVNASVLHILKYLTGSAKTYANSVQAYVDVKDVALAHILLYE--TPEAS 246

Query: 291 GRYICFDRVI---STVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRS 342
           GRY+C + V+     V+  +K   E  +P     + T P     P++ SN+K+ DL    
Sbjct: 247 GRYLCAESVLHRGDVVEILSKFFPEYPIPT-KCSDVTKP--RVKPYKFSNQKLKDLGLEF 306

BLAST of Carg04438 vs. ExPASy Swiss-Prot
Match: Q6K9A2 (Cinnamoyl-CoA reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.1e-26
Identity = 94/314 (29.94%), Postives = 143/314 (45.54%), Query Frame = 0

Query: 46  DDAEEKVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNK--LDETTSGRPNVTVV 105
           ++ E+++VCVT    F+GS +V ELL+ GY VR    +P D     L E       +++ 
Sbjct: 11  NNGEKQLVCVTGAGGFIGSWVVKELLIRGYHVRGTARDPADSKNAHLLELEGADERLSLC 70

Query: 106 VAKLTDVNSLVEAFNGCRGVFHTSSSI--DP-------AGLSGYTACARTETVRNCVLTS 165
            A + D  SL  AF+GC GVFH +S +  DP        G       A    VR  V TS
Sbjct: 71  RADVLDAASLRAAFSGCHGVFHVASPVSNDPDLVPVAVEGTRNVINAAADMGVRRVVFTS 130

Query: 166 SLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKL 225
           S  A        +     V+D  CWS ++ C+     +   K+ +E  A + A + GL+L
Sbjct: 131 SYGAV---HMNPNRSPDAVLDETCWSDYEFCKQTDNLYCCAKMMAEMTATEEAAKRGLEL 190

Query: 226 ATICSPLITGSNFHSR---NSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELK 285
           A +   +  G         ++     YL G ++ Y N + A VDV  +A+AHV +YE  +
Sbjct: 191 AVVVPSMTMGPMLQQTLNFSTNHVARYLMGTKKSYPNAVAAYVDVRDVARAHVLVYE--R 250

Query: 286 ENGSGRYICFDRVISTVDEATKLAAEI--RVPVGTIVETTLPIDSPI--PFQLSNKKVSD 342
               GRY+C   V+    E  ++  E+  R P     E       P+  P++ SN+++ D
Sbjct: 251 PEARGRYLCIGTVLHRA-ELLRMLRELFPRYPATAKCEDD---GKPMAKPYKFSNQRLKD 310

BLAST of Carg04438 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-24
Identity = 82/274 (29.93%), Postives = 135/274 (49.27%), Query Frame = 0

Query: 45  IDDAEEKVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNKLD---ETTSGRPNVT 104
           +D A+ K VCVT    FL S +V  LL+ GY V   V +P +  KL    +    +  + 
Sbjct: 1   MDQAKGK-VCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGNEKKLAHLWKLEGAKERLR 60

Query: 105 VVVAKLTDVNSLVEAFNGCRGVFHTSSSI------------DPA---GLSGYTACARTET 164
           +V A L +  S   A  GC+GVFHT+S +             PA    L+   +C +  +
Sbjct: 61  LVKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPS 120

Query: 165 VRNCVLTSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKI 224
           ++  VLTSS      RD  +       +D   W+S +LC+  ++W+AL K  +E+ AWK 
Sbjct: 121 LKRVVLTSSSSTVRIRDDFD---PKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKF 180

Query: 225 AQESGLKLATICSPLITGSNFH---SRNSTPTMAYLKGAREMYK-NGLLATVDVTKLAKA 284
           ++E+G+ L T+    + G +        ++  +  LKG  E ++ +G +  V +  +A+ 
Sbjct: 181 SEENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVART 240

Query: 285 HVCIYEELKENGSGRYICFDRVISTVDEATKLAA 297
           H+ ++E   E   GRYIC   VIS  +  + L+A
Sbjct: 241 HIVVFEH--EAAQGRYICSSNVISLEELVSFLSA 268

BLAST of Carg04438 vs. ExPASy Swiss-Prot
Match: Q9XES5 (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica OX=3750 GN=DFR PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 5.0e-20
Identity = 92/310 (29.68%), Postives = 141/310 (45.48%), Query Frame = 0

Query: 48  AEEKVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNP---EDVNKLDETTSGRPNVTVVV 107
           +E + VCVT    F+GS +V  LL HGY+VR  V +P   + V  L +      ++T+  
Sbjct: 3   SESESVCVTGASGFIGSWLVMRLLEHGYTVRATVRDPTNQKKVKHLLDLPKAETHLTLWK 62

Query: 108 AKLTDVNSLVEAFNGCRGVFHTSS-----SIDPAG----------LSGYTACARTETVRN 167
           A L D  S  EA  GC GVFH ++     S DP            L    AC + +TVR 
Sbjct: 63  ADLADEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRK 122

Query: 168 CVLTSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKL--W-FALGKLKSEKVAWKI 227
            V TSS       +  +      V D   WS  + C+  K+  W + + K  +E+ AWK 
Sbjct: 123 LVFTSSAGTVNVEEHQKP-----VYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKY 182

Query: 228 AQESGLKLATICSPLITGSNFHSRNSTPTMAYLKGAREMYKN----GLLAT---VDVTKL 287
           A+E+ +   TI   L+ G  F   +  P++  + G   + +N    G++     V +  L
Sbjct: 183 AKENNIDFITIIPTLVIGP-FLMPSMPPSL--ITGLSPILRNESHYGIIKQGQYVHLDDL 242

Query: 288 AKAHVCIYEELKENGSGRYICFDRVISTVDEATKLAAEIRVPVGTIVETTLPIDSPI-PF 329
             +H+ +YE  K    GRYIC     +T+ E  K+  E + P   I      ID  + P 
Sbjct: 243 CLSHIYLYEHPK--AEGRYICSSH-DATIHELVKMLRE-KYPEYNIPTKFKGIDDNLEPV 300

BLAST of Carg04438 vs. ExPASy TrEMBL
Match: A0A6J1GQ53 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111456087 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.1e-190
Identity = 344/361 (95.29%), Postives = 345/361 (95.57%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAGLSGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240
           PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG
Sbjct: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240

Query: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300
           SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV
Sbjct: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300

Query: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 346
           ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSN+GRSCLNENRF
Sbjct: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNLGRSCLNENRF 360

BLAST of Carg04438 vs. ExPASy TrEMBL
Match: A0A6J1JPH8 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111487721 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 2.9e-188
Identity = 340/361 (94.18%), Postives = 343/361 (95.01%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEF+SLKSKLIDDAEEKVVCVTSGVS
Sbjct: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFRSLKSKLIDDAEEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDPAGLSGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPAGLSGYTKVMSEVEMRSTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240
           PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG
Sbjct: 181 PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLITG 240

Query: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFDRV 300
           SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVC+YEELKENGSGRYICFDRV
Sbjct: 241 SNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCVYEELKENGSGRYICFDRV 300

Query: 301 ISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 346
           ISTVDEATKLA EI+VPV TIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF
Sbjct: 301 ISTVDEATKLAVEIKVPVETIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNENRF 360

BLAST of Carg04438 vs. ExPASy TrEMBL
Match: A0A6J1K2V0 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111489565 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 2.5e-155
Identity = 288/361 (79.78%), Postives = 311/361 (86.15%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGV+ R EET VSELE+ RR LVAC G+QRRK G+EFKSLK K IDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRYEETFVSELERCRRMLVACAGVQRRKGGKEFKSLKPKPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNEL +HGYSVRVIVDNPEDVNKL+ETTSGR NVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYSVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+G+SGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMKTTENVMQACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 ---PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPL 240
               ES G+SR+VDH CWSS QLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICS L
Sbjct: 181 AVESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 ITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICF 300
           +TG NFH R+ST T+AYLKGAREMY NGLLATVDVTKLAKAHVC+YE +KEN S RYICF
Sbjct: 241 LTGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNE 343
           DRVIST +EAT LAAEI VPVG+IV+TT PIDSP+ FQLSNKK+SDLM RSN+ RSCLNE
Sbjct: 301 DRVISTPNEATMLAAEIGVPVGSIVQTTPPIDSPVSFQLSNKKLSDLMFRSNLQRSCLNE 360

BLAST of Carg04438 vs. ExPASy TrEMBL
Match: A0A6J1FEE8 (cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111444756 PE=3 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 3.6e-154
Identity = 285/360 (79.17%), Postives = 309/360 (85.83%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGV+ RSEET VSELE+ RR LVAC G+QRRKDG+EFKSLK + IDDA+EKVVCVTSGVS
Sbjct: 1   MGVVVRSEETFVSELERCRRMLVACAGVQRRKDGKEFKSLKPRPIDDADEKVVCVTSGVS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLGSAIVNEL +HGY VRVIVDNPEDVNKL+ETTSGR NVTVVVA LTDVNSLVEAFNGC
Sbjct: 61  FLGSAIVNELSLHGYFVRVIVDNPEDVNKLEETTSGRHNVTVVVADLTDVNSLVEAFNGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+G+SGYT                ACARTETVRNCVLTSSLLACIWRDQ
Sbjct: 121 RGVFHTSSSIDPSGVSGYTKVMSEVEMRTTENVMEACARTETVRNCVLTSSLLACIWRDQ 180

Query: 181 ---PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPL 240
               ES G+SR+VDH CWSS QLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICS L
Sbjct: 181 AGESESIGSSRIVDHACWSSLQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSAL 240

Query: 241 ITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICF 300
           ITG NFH R+ST T+AYLKGAREMY NGLLATVDVTKLAKAHVC+YE +KEN S RYICF
Sbjct: 241 ITGPNFHRRSSTATIAYLKGAREMYTNGLLATVDVTKLAKAHVCVYEGMKENSSSRYICF 300

Query: 301 DRVISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNE 342
           DRVIST +EA+ LA EI VPVG+IV+TT PI+SP+ FQLSNKK+ DLM RSN+ RSCLNE
Sbjct: 301 DRVISTANEASMLATEIGVPVGSIVQTTPPINSPVSFQLSNKKLCDLMFRSNLQRSCLNE 360

BLAST of Carg04438 vs. ExPASy TrEMBL
Match: A0A6J1BZP5 (cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111006177 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.0e-152
Identity = 279/362 (77.07%), Postives = 313/362 (86.46%), Query Frame = 0

Query: 1   MGVLFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKSKLIDDAEEKVVCVTSGVS 60
           MGV+FR+EETLV ELEQLRRKLVACGGIQRRKDG+E+++LKSK IDDAEEK+VCVTSG S
Sbjct: 1   MGVVFRAEETLVLELEQLRRKLVACGGIQRRKDGDEYRNLKSKPIDDAEEKIVCVTSGAS 60

Query: 61  FLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTSGRPNVTVVVAKLTDVNSLVEAFNGC 120
           FLG+A+VNELL+HGYSVRVIVDNPEDV+KL+ET S R +VTVV AKLTDV SLVEAF+GC
Sbjct: 61  FLGAALVNELLLHGYSVRVIVDNPEDVSKLEETMSNRHDVTVVAAKLTDVESLVEAFDGC 120

Query: 121 RGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLTSSLLACIWRDQ 180
           RGVFHTSSSIDP+GLSGYT                AC RTETVRNCVLTSSLLACIW+DQ
Sbjct: 121 RGVFHTSSSIDPSGLSGYTKIMSEVEMKTTENVMEACTRTETVRNCVLTSSLLACIWQDQ 180

Query: 181 --PESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLKLATICSPLI 240
                + TSRVVDHGCWSS QLCQDKKLW+ALGKLKSEKVAW++AQESGLKLATICS LI
Sbjct: 181 AGESESETSRVVDHGCWSSLQLCQDKKLWYALGKLKSEKVAWRMAQESGLKLATICSALI 240

Query: 241 TGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEELKENGSGRYICFD 300
           TG NFH RNSTPTMAYLKGAREMY NG+LATV+VTKLAKAHVC+YE +KEN SGRYICFD
Sbjct: 241 TGPNFHRRNSTPTMAYLKGAREMYANGVLATVEVTKLAKAHVCVYEGMKENASGRYICFD 300

Query: 301 RVISTVDEATKLAAEIRVPVGTIVETTLPIDSPIPFQLSNKKVSDLMSRSNVGRSCLNEN 345
           RVISTV EA KLAAE+ VPV T+++TT P  S IPFQLSNKK+S LMSR+++ R+CLNE+
Sbjct: 301 RVISTVGEAEKLAAEMAVPVTTMLQTTPPTHSSIPFQLSNKKLSHLMSRTDLPRTCLNES 360

BLAST of Carg04438 vs. TAIR 10
Match: AT5G14700.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 4.4e-88
Identity = 181/374 (48.40%), Postives = 241/374 (64.44%), Query Frame = 0

Query: 4   LFRSEETLVSELEQLRRKLVACGGIQRRKDGEEFKSLKS--------KLIDDAEEKVVCV 63
           + R+ ET  +EL++       C  +QRRKD + F+  +          L  DA  ++VCV
Sbjct: 3   IVRATETFSAELKEF-----MCAAVQRRKDDDGFRGSRGGGKSRNAMDLDSDAGNRLVCV 62

Query: 64  TSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNKLD------ETTSGRPNVTVVVAKLTD 123
           T GVS+LG AIV  LLVHGYSVR++VD PED  K+       ET S    +T VV++LT+
Sbjct: 63  TGGVSYLGRAIVKRLLVHGYSVRIVVDCPEDKEKVSEMEADAETASFSNMITSVVSRLTE 122

Query: 124 VNSLVEAFNGCRGVFHTSSSIDPAGLSGYT----------------ACARTETVRNCVLT 183
           ++SL++AF+GC GVFHTS+ +DPAG+SGY+                AC RT +VR CV T
Sbjct: 123 IDSLIKAFDGCAGVFHTSAFVDPAGVSGYSKSMAELEAKVSESVIEACTRTASVRKCVFT 182

Query: 184 SSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGLK 243
           SSLLAC W+  P ++    V++   WS  QLC D KLW+ALGKLK+EK AW+IA   GLK
Sbjct: 183 SSLLACAWQKNPCNSLDHSVINEESWSDEQLCIDNKLWYALGKLKAEKAAWRIADSKGLK 242

Query: 244 LATICSPLITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEEL-KE 303
           LATIC  LITG +F +RNST T+AYLKGA+EMY NGLLAT+DV +LAKAHVC++E L  +
Sbjct: 243 LATICPALITGPDFFNRNSTSTLAYLKGAKEMYSNGLLATMDVNRLAKAHVCLWEGLGNK 302

Query: 304 NGSGRYICFDRVISTVDEATKLAAEIRVPV----GTIVETTLPIDSPIPFQLSNKKVSDL 343
              GRYICFD ++S  D A KLA +I V +    G   ++    ++    Q+S+KK+ DL
Sbjct: 303 TAFGRYICFDTILSR-DGAEKLAKDIDVQIEKICGNSNDSDANTETEASLQISDKKLLDL 362

BLAST of Carg04438 vs. TAIR 10
Match: AT2G23910.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 7.7e-40
Identity = 101/301 (33.55%), Postives = 155/301 (51.50%), Query Frame = 0

Query: 49  EEKVV---CVTSGVSFLGSAIVNELLVHGYSVRVIV-DNPEDV--NKLDETTSGRPNVTV 108
           +EK +   CV    +++G  I+  LL  GYSV   +  N E V   K+ +  +    + V
Sbjct: 3   QEKTISCCCVLDASTYVGFWILKRLLTRGYSVHAAIRKNGESVLEEKIRDMEANEERLEV 62

Query: 109 VVAKLTDVNSLVEAFNGCRGVF---------HTSSSID---PAGLSGYTACARTETVRNC 168
               + D  S++ + N C  VF              +D      ++   ACARTE++   
Sbjct: 63  YDVDVLDYQSILISLNNCNAVFCCLDNPEGYDEEKEVDLEVRGAINVVEACARTESIEKI 122

Query: 169 VLTSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQES 228
           V +SSL A IWRD   + GT + VD  CWS    C  KKLW AL K +SEK AW +A + 
Sbjct: 123 VFSSSLTAAIWRD---NIGTQKDVDEKCWSDLDFCLKKKLWHALAKTQSEKAAWALAMDR 182

Query: 229 GLKLATICSPLITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEEL 288
            + + ++   LI G +    N  PTM+YLKGA +MY+NG+LA VDV  +A  H+  +E+ 
Sbjct: 183 MVNMVSVNPGLIVGPSVAQHNPRPTMSYLKGAAQMYENGVLAYVDVEFVADVHIRAFED- 242

Query: 289 KENGSGRYICFDRVISTVDEATKLAAEIR--VPVGTIVETTLPIDSPIPFQLSNKKVSDL 330
             +  GRY CF+++++T +EA KL   +   +P+    E  +        +L NKK++ L
Sbjct: 243 -TSACGRYFCFNQIVNTEEEALKLVQTLSPLIPMPPRHEKEMQGSEVYEERLRNKKLNKL 298

BLAST of Carg04438 vs. TAIR 10
Match: AT4G30470.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.0e-39
Identity = 103/310 (33.23%), Postives = 158/310 (50.97%), Query Frame = 0

Query: 47  DAEEKVVCVTSGVSFLGSAIVNELLVHGYSVRVIV-----DNPEDVNKLDETTSGRPNVT 106
           D      CV    +++G  I+ +LL  GYSV   +        E++ +  ETT  R  + 
Sbjct: 4   DKSTSCCCVLDASTYVGFWILKKLLSRGYSVHAAIRRNGESEIEEMIREMETTEER--LV 63

Query: 107 VVVAKLTDVNSLVEAFNGCRGVF--------HTSSSID---PAGLSGYTACARTETVRNC 166
           V    + D  S++ +   C  VF        +    +D      ++   AC RTE++   
Sbjct: 64  VYDVDVLDYQSILVSLKTCNVVFCCLDSPEGYDEKEVDLEVRGAINVVEACGRTESIEKI 123

Query: 167 VLTSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQES 226
           V +SSL A IWRD   + GT + VD  CWS    C+ KKLW AL K+ SEK AW +A + 
Sbjct: 124 VFSSSLTASIWRD---NIGTQKDVDEKCWSDQDFCRSKKLWHALAKMLSEKAAWALAMDR 183

Query: 227 GLKLATICSPLITGSNFHSRNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEEL 286
            L + +I   L+ G +    N+ PTM+YLKGA +MY+NG+LA VDV  LA  H+  +E++
Sbjct: 184 RLNMVSINPGLVVGPSVAQHNARPTMSYLKGAAQMYENGVLAYVDVKFLADVHIRAFEDV 243

Query: 287 KENGSGRYICFDRVISTVDEATKLAAEIR--VPVGTIVETTLPIDSPIPFQLSNKKVSDL 339
             +  GRY CF+++++T +EA KL   +   +P+    E  +        +L N K+S L
Sbjct: 244 --SACGRYFCFNQIVNTEEEALKLVESLSPLIPMPPRYENEMHGSEVYEERLRNNKLSKL 303

BLAST of Carg04438 vs. TAIR 10
Match: AT1G51410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 4.4e-27
Identity = 81/257 (31.52%), Postives = 126/257 (49.03%), Query Frame = 0

Query: 49  EEKVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTS---GRPNVTVVVA 108
           EEK VCVT    ++ S IV  LL+ GY+V+  V +P D  K +   +       + +  A
Sbjct: 5   EEKTVCVTGASGYIASWIVKLLLLRGYTVKASVRDPNDPRKTEHLLALEGAEERLKLFKA 64

Query: 109 KLTDVNSLVEAFNGCRGVFHTSSS------------IDPA---GLSGYTACARTETVRNC 168
            L +  S   A +GC GVFHT+S             +DPA    ++  ++C +T +V+  
Sbjct: 65  NLLEEGSFDSAIDGCEGVFHTASPFYHDVKDPQAELLDPAVKGTINVLSSCLKTSSVKRV 124

Query: 169 VLTSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQES 228
           VLTSS+ A  +   P +  T  +VD   ++    C+  KLW+ L K  +E  AWK A+E+
Sbjct: 125 VLTSSIAAVAFNGMPRTPET--IVDETWFADPDYCRASKLWYVLSKTLAENAAWKFAKEN 184

Query: 229 GLKLATICSPLITGSNFHSRNSTPTMAYL---KGAREMYKNGLLATVDVTKLAKAHVCIY 285
            L+L +I   ++ G       +T   A L   KGA + + N     V+V  +A AH+  +
Sbjct: 185 NLQLVSINPAMVIGPLLQPTLNTSAAAVLSLIKGA-QTFPNATFGWVNVKDVANAHIQAF 244

BLAST of Carg04438 vs. TAIR 10
Match: AT1G09510.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 9.7e-27
Identity = 93/300 (31.00%), Postives = 146/300 (48.67%), Query Frame = 0

Query: 51  KVVCVTSGVSFLGSAIVNELLVHGYSVRVIVDNPEDVNKLDETTS---GRPNVTVVVAKL 110
           K+VCVT    ++ S IV  LL+ GY+VR  V +P D  K +   +    +  + +  A L
Sbjct: 6   KMVCVTGASGYVASWIVKLLLLRGYTVRATVRDPSDEKKTEHLLALDGAKEKLKLFKADL 65

Query: 111 TDVNSLVEAFNGCRGVFHTSSS------------IDPA---GLSGYTACARTETVRNCVL 170
            +  S  +A  GC  VFHT+S             IDPA    L+    CA+  +V+  ++
Sbjct: 66  LEEGSFEQAIEGCDAVFHTASPVSLTVTDPQIELIDPAVKGTLNVLKTCAKVSSVKRVIV 125

Query: 171 TSSLLACIWRDQPESTGTSRVVDHGCWSSFQLCQDKKLWFALGKLKSEKVAWKIAQESGL 230
           TSS+ A ++R+   + G + +VD  C+S    C +KKLW+AL K  +E  AW+ A+E GL
Sbjct: 126 TSSMAAVLFRE--PTLGPNDLVDESCFSDPNFCTEKKLWYALSKTLAEDEAWRFAKEKGL 185

Query: 231 KLATICSPLITGSNFHS--RNSTPTMAYLKGAREMYKNGLLATVDVTKLAKAHVCIYEEL 290
            L  I   L+ G         S   +  L   ++ + N     VDV  +A AH+  +E  
Sbjct: 186 DLVVINPGLVLGPLLKPSLTFSVNVIVELITGKDNFINKDFRLVDVRDVALAHIKAFETP 245

Query: 291 KENGSGRYICFDRVISTVDEATKLAAEI--RVPVGTIVETTLPIDSPIPFQLSNKKVSDL 329
             N  GRYI    V+ T+++  K+  E    + +G   E +  I  P+ ++L  +KV  L
Sbjct: 246 SAN--GRYIIEGPVV-TINDIEKILREFFPDLNLGNKGEASEII--PVIYKLCVEKVKSL 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033115.12.8e-193100.00Cinnamoyl-CoA reductase-like SNL6 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6602435.13.8e-19095.57Cinnamoyl-CoA reductase-like SNL6, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022953600.18.4e-19095.29cinnamoyl-CoA reductase-like SNL6 [Cucurbita moschata][more]
XP_022990996.16.0e-18894.18cinnamoyl-CoA reductase-like SNL6 [Cucurbita maxima][more]
XP_023528175.14.3e-18693.63cinnamoyl-CoA reductase-like SNL6 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q0JKZ06.9e-7041.76Cinnamoyl-CoA reductase-like SNL6 OS=Oryza sativa subsp. japonica OX=39947 GN=SN... [more]
A0A059TC021.4e-3333.98Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1[more]
Q6K9A22.1e-2629.94Cinnamoyl-CoA reductase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CCR1 PE=1 ... [more]
Q500U81.5e-2429.93Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
Q9XES55.0e-2029.68Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestic... [more]
Match NameE-valueIdentityDescription
A0A6J1GQ534.1e-19095.29cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111456087 ... [more]
A0A6J1JPH82.9e-18894.18cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111487721 PE... [more]
A0A6J1K2V02.5e-15579.78cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita maxima OX=3661 GN=LOC111489565 PE... [more]
A0A6J1FEE83.6e-15479.17cinnamoyl-CoA reductase-like SNL6 OS=Cucurbita moschata OX=3662 GN=LOC111444756 ... [more]
A0A6J1BZP52.0e-15277.07cinnamoyl-CoA reductase-like SNL6 OS=Momordica charantia OX=3673 GN=LOC111006177... [more]
Match NameE-valueIdentityDescription
AT5G14700.14.4e-8848.40NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G23910.17.7e-4033.55NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G30470.11.0e-3933.23NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G51410.14.4e-2731.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09510.19.7e-2731.00NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 62..157
e-value: 8.0E-9
score: 35.6
NoneNo IPR availableGENE3D3.40.50.720coord: 42..341
e-value: 4.7E-53
score: 182.5
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 38..340
NoneNo IPR availablePANTHERPTHR10366:SF483CINNAMOYL COA REDUCTASE-LIKE PROTEINcoord: 38..340
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 49..306

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04438-RACarg04438-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010190 cytochrome b6f complex assembly
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity