Carg03523 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03523
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	disease resistance protein RGA2-like
Location	Carg_Chr14: 2242946 .. 2245354 (-)
RNA-Seq Expression	Carg03523
Synteny	Carg03523

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTATACCATGTTGAGCCTTAAATCAATTCTTTTTGATGCTGAAGAGAAGCAAGAACAAGAACAGAATCATGATCTACGTAAGTGGCTTAAGGAGCTTCAAAACACCTTTTACCAAATTGAGGACACCATGGATGAATTCAAATGGGATTTTGAACAAGAGGGCACTGGAAAAAAGGTACAAGCCCCTTTCTCGTGCTCTCACCCAATTTCTGCACATAAATTTAAACGGTTTCGTAAAATGACGAGACTACGCAATCATTTGGATACACTAGCAGCCAGAATGTATGAATTTCATCTCACTGAAAAGCACCCAGATTCCATAGGAAGAATGAAGACAGCACACTCCTTCCTTAGCACTACTGAAGTTTCAGCAAGACATATGAAACCAAGTTGGCAATTGCAGTACACCTTAACTGATGCTCCAATGGCTTATGATGGCAATCGGTATTCTGAACTTCTGAGAACTTTCAAATCTATTCGTGGGTTCTTCCTCATAGTTGGGGAAGCAGGTATAGGTAAGAGCACACTTGCCAAATTCCTTTACAATGATAAAGAAGTAGTTGAACAGTATCTTTCAAGATTCTGGGTTTGTGTGAAAGAAGGTTTTGATACACAGAGATTGATCGAAGAGGTATATAGTGTTGTAGCCTGTAAAGAAATTTGTAATGACTTGACTAAGAAACAATTGCATTTAAAGGTTCAACAACTTCTGAAGGGTACATCTTTTTTGCTCGTCATTCAAGACCTTTCGACCACAAACTTAGACGATTCATCCGCATTGGTGGAACTATTGGGCATGGGAAACAAGAACAGCAAAATCTCAGTGACCACTCAAAACAGCAAAATCTTAGTGACCACTCAAAGTGAGGAGACTGCAGGTGCTCTAGGACTAGGAATAGTCTATGCGTCTGAGAAACAGTCGGAGAAACAATCGACGCAGAATTCGGACGATAAGAATCAGAAAGTTTCAACAGTCTTCAAACTGGAGAGGCTGTCAGAGGATAAATCATCTAAGTTATTCAAAAGATATGCCTTCAGAGATAGACAAGCAGTAAACAATGAAGAATTCACTGGAATAGAAGACCAAATTCTGCAGAAATGCAAGGGAGTTCCGTTGGCAATAAAGTGTATGGGAAGCTTGCTATCTTCAAAAACTAGAATAGCTGAGTGGAAGAATATCAAGGACAAGTTGTGGGCACAAAAGGAAGAGAATGACGGTATTTTACACATACTCAGATTATGCTATAATCAAATGCCCTCACACCTGAAGCCCTGTTTTCTTCATTGTGCTCAATTACCAGACCGCATATTATCTTCAAATGACGTGATTCAGTTATGGATGGCACATGGGCTCCTCCATTCACCTGAAGAGAATCACGCATCTATGAAAAATATAGGTGAGAAGTATTTCATGGAGCTATGGTCAAGATGTTTCGTTCAAGAAATTGAAGAACATGGCCTCGGCTACTGGTTTAAACTGCACCCTCTTACCCAAAAACTTGCACACGTACTCGCACAAGAACAGTCTGAGGGCTTAGTAGGTACCAAACCAGTCACTGAAATAAAATCCCTAGCCTTCCATGTAAGAGATATGGTGCCACCTAATGCATCCCTACCTGGAATGTGCATTCTGAAGTACAACAGCTTAAGAGTGTTACATTTAGGCAATGCAAACATAGAGGAAATTCCAAATGCTATAGGAACACTTACAAGCCTGAGATACCTCGACTTGCATGGAAATAAGAACATCAAGCGGCTGCCGAATTCAATCTGCAATCTACAAAGTTTGCAAACCTTGATTCTTGAATCTTGTTCCGAACTTAAAGAATTGCCAACTGATATAAGGAATTTGATCAGCCTGAAATACTTGTGGATAACAACAAAAGAGCTTTATTTGCACAAAAACGGAGTTGGAACCATGACTTCTCTGCAATTTCTCGCCATTGGAGGGTGCAAAAACCTAAAAATTCTATTCGAACGGCCAGAGTGCCTTGCAGACCTTGAAACCCTAATGATTTACAATTGCAATACTTTGAAATCGTTGCCAAATGAGATGATACATCTAAAATCACTACAGAATTTGGTTATCTGGGGCTGCAAGCAACTAACACTCACCTTAGAGAACGCCGAATTTAGGCTTCAGAGATTCACAATCAGAGAGCTTCCAGGAGTGGATGAATTACCTGAATGGCTTGACGGATCGATCGAAACTTTGAGAAACCTGCAGATCATCAATTGCCCCATAACCATCAATGTAGGGAGGTTACAAGCGTTTACAGGTGTTGAAACGGCCAAAATTGTAGGATCTGTAAAAGTCAAAGCGGCGGCGCCGGGTTATGGCTTCCAATATTGGAATACGGTGATACGTGGTTCGAGCGGGAGTGGCAGCAGAAAATATTACTAA

mRNA sequence

ATGGAGTATACCATGTTGAGCCTTAAATCAATTCTTTTTGATGCTGAAGAGAAGCAAGAACAAGAACAGAATCATGATCTACGTAAGTGGCTTAAGGAGCTTCAAAACACCTTTTACCAAATTGAGGACACCATGGATGAATTCAAATGGGATTTTGAACAAGAGGGCACTGGAAAAAAGGTACAAGCCCCTTTCTCGTGCTCTCACCCAATTTCTGCACATAAATTTAAACGGTTTCGTAAAATGACGAGACTACGCAATCATTTGGATACACTAGCAGCCAGAATGTATGAATTTCATCTCACTGAAAAGCACCCAGATTCCATAGGAAGAATGAAGACAGCACACTCCTTCCTTAGCACTACTGAAGTTTCAGCAAGACATATGAAACCAAGTTGGCAATTGCAGTACACCTTAACTGATGCTCCAATGGCTTATGATGGCAATCGGTATTCTGAACTTCTGAGAACTTTCAAATCTATTCGTGGGTTCTTCCTCATAGTTGGGGAAGCAGGTATAGGTAAGAGCACACTTGCCAAATTCCTTTACAATGATAAAGAAGTAGTTGAACAGTATCTTTCAAGATTCTGGGTTTGTGTGAAAGAAGGTTTTGATACACAGAGATTGATCGAAGAGGTATATAGTGTTGTAGCCTGTAAAGAAATTTGTAATGACTTGACTAAGAAACAATTGCATTTAAAGGTTCAACAACTTCTGAAGGGTACATCTTTTTTGCTCGTCATTCAAGACCTTTCGACCACAAACTTAGACGATTCATCCGCATTGGTGGAACTATTGGGCATGGGAAACAAGAACAGCAAAATCTCAGTGACCACTCAAAACAGCAAAATCTTAGTGACCACTCAAAGTGAGGAGACTGCAGGTGCTCTAGGACTAGGAATAGTCTATGCGTCTGAGAAACAGTCGGAGAAACAATCGACGCAGAATTCGGACGATAAGAATCAGAAAGTTTCAACAGTCTTCAAACTGGAGAGGCTGTTACAAGCGTTTACAGGTGTTGAAACGGCCAAAATTGTAGGATCTGTAAAAGTCAAAGCGGCGGCGCCGGGTTATGGCTTCCAATATTGGAATACGGTGATACGTGGTTCGAGCGGGAGTGGCAGCAGAAAATATTACTAA

Coding sequence (CDS)

ATGGAGTATACCATGTTGAGCCTTAAATCAATTCTTTTTGATGCTGAAGAGAAGCAAGAACAAGAACAGAATCATGATCTACGTAAGTGGCTTAAGGAGCTTCAAAACACCTTTTACCAAATTGAGGACACCATGGATGAATTCAAATGGGATTTTGAACAAGAGGGCACTGGAAAAAAGGTACAAGCCCCTTTCTCGTGCTCTCACCCAATTTCTGCACATAAATTTAAACGGTTTCGTAAAATGACGAGACTACGCAATCATTTGGATACACTAGCAGCCAGAATGTATGAATTTCATCTCACTGAAAAGCACCCAGATTCCATAGGAAGAATGAAGACAGCACACTCCTTCCTTAGCACTACTGAAGTTTCAGCAAGACATATGAAACCAAGTTGGCAATTGCAGTACACCTTAACTGATGCTCCAATGGCTTATGATGGCAATCGGTATTCTGAACTTCTGAGAACTTTCAAATCTATTCGTGGGTTCTTCCTCATAGTTGGGGAAGCAGGTATAGGTAAGAGCACACTTGCCAAATTCCTTTACAATGATAAAGAAGTAGTTGAACAGTATCTTTCAAGATTCTGGGTTTGTGTGAAAGAAGGTTTTGATACACAGAGATTGATCGAAGAGGTATATAGTGTTGTAGCCTGTAAAGAAATTTGTAATGACTTGACTAAGAAACAATTGCATTTAAAGGTTCAACAACTTCTGAAGGGTACATCTTTTTTGCTCGTCATTCAAGACCTTTCGACCACAAACTTAGACGATTCATCCGCATTGGTGGAACTATTGGGCATGGGAAACAAGAACAGCAAAATCTCAGTGACCACTCAAAACAGCAAAATCTTAGTGACCACTCAAAGTGAGGAGACTGCAGGTGCTCTAGGACTAGGAATAGTCTATGCGTCTGAGAAACAGTCGGAGAAACAATCGACGCAGAATTCGGACGATAAGAATCAGAAAGTTTCAACAGTCTTCAAACTGGAGAGGCTGTTACAAGCGTTTACAGGTGTTGAAACGGCCAAAATTGTAGGATCTGTAAAAGTCAAAGCGGCGGCGCCGGGTTATGGCTTCCAATATTGGAATACGGTGATACGTGGTTCGAGCGGGAGTGGCAGCAGAAAATATTACTAA

Protein sequence

MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKKVQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLGIVYASEKQSEKQSTQNSDDKNQKVSTVFKLERLLQAFTGVETAKIVGSVKVKAAAPGYGFQYWNTVIRGSSGSGSRKYY

Homology

[Close]

BLAST of Carg03523 vs. NCBI nr
Match: KAG7017603.1 (Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 741.5 bits (1913), Expect = 3.6e-210
Identity = 379/379 (100.00%), Postives = 379/379 (100.00%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60
           MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60

Query: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120
           VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS
Sbjct: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120

Query: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 180
           TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK
Sbjct: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 180

Query: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240
           FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK
Sbjct: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240

Query: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG 300
           GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG
Sbjct: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG 300

Query: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERLLQAFTGVETAKIVGSVKVKAAAPGYGF 360
           IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERLLQAFTGVETAKIVGSVKVKAAAPGYGF
Sbjct: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERLLQAFTGVETAKIVGSVKVKAAAPGYGF 360

Query: 361 QYWNTVIRGSSGSGSRKYY 380
           QYWNTVIRGSSGSGSRKYY
Sbjct: 361 QYWNTVIRGSSGSGSRKYY 379

[Close]

BLAST of Carg03523 vs. NCBI nr
Match: KAG6580847.1 (Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 648.3 bits (1671), Expect = 4.2e-182
Identity = 331/333 (99.40%), Postives = 332/333 (99.70%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60
           MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60

Query: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120
           VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS
Sbjct: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120

Query: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 180
           TTEVSARHMKPSWQLQYTLTDAPMAYDGNRY+ELLRTFKSIRGFFLIVGEAGIGKSTLAK
Sbjct: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYAELLRTFKSIRGFFLIVGEAGIGKSTLAK 180

Query: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240
           FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK
Sbjct: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240

Query: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG 300
           GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKI VTTQNSKILVTTQSEETAGALGLG
Sbjct: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKILVTTQNSKILVTTQSEETAGALGLG 300

Query: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 334
           IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL
Sbjct: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 333

[Close]

BLAST of Carg03523 vs. NCBI nr
Match: KAG6580847.1 (Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 93.2 bits (230), Expect = 5.2e-15
Identity = 45/46 (97.83%), Postives = 45/46 (97.83%), Query Frame = 0


Query: 334 LQAFTGVETAKIVGSVKVKAAAPGYGFQYWNTVIRGSSGSGSRKYY 380
           LQAFTGVETAKIVGSVKVK AAPGYGFQYWNTVIRGSSGSGSRKYY
Sbjct: 679 LQAFTGVETAKIVGSVKVKGAAPGYGFQYWNTVIRGSSGSGSRKYY 724

HSP 2 Score: 605.9 bits (1561), Expect = 2.4e-169
Identity = 312/333 (93.69%), Postives = 315/333 (94.59%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60
           MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60

Query: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120
           VQAPFSCSHPISAHKF RFRKM RLRNHLDTLAARMYEFHLTEKHPDSIGR KTAHSFLS
Sbjct: 61  VQAPFSCSHPISAHKFIRFRKMMRLRNHLDTLAARMYEFHLTEKHPDSIGRRKTAHSFLS 120

Query: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 180
           TTEVS RHMKPSWQLQYTLTDAPM YDG+RY+ELL TFKSI GFFLIVGEAGIGKSTLAK
Sbjct: 121 TTEVSVRHMKPSWQLQYTLTDAPMTYDGDRYAELLTTFKSIHGFFLIVGEAGIGKSTLAK 180

Query: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240
           FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQ LLK
Sbjct: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQHLLK 240

Query: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG 300
           GTSFLLVIQDLSTTNLDDSS L+ELLGMGNK         NSKILVTTQSEETAGALGLG
Sbjct: 241 GTSFLLVIQDLSTTNLDDSSTLMELLGMGNK---------NSKILVTTQSEETAGALGLG 300

Query: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 334
           IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL
Sbjct: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 324

[Close]

BLAST of Carg03523 vs. NCBI nr
Match: XP_022934599.1 (disease resistance protein RGA2-like [Cucurbita moschata])

HSP 1 Score: 93.2 bits (230), Expect = 5.2e-15
Identity = 45/46 (97.83%), Postives = 45/46 (97.83%), Query Frame = 0


Query: 334 LQAFTGVETAKIVGSVKVKAAAPGYGFQYWNTVIRGSSGSGSRKYY 380
           LQAFTGVETAKIVGSVKVK AAPGYGFQYWNTVIRGSSGSGSRKYY
Sbjct: 748 LQAFTGVETAKIVGSVKVKGAAPGYGFQYWNTVIRGSSGSGSRKYY 793

HSP 2 Score: 582.4 bits (1500), Expect = 2.8e-162
Identity = 302/333 (90.69%), Postives = 310/333 (93.09%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60
           MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD EQEGTGKK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDSEQEGTGKK 60

Query: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120
           VQAPFSCS PISAHKF RFRKM RLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS
Sbjct: 61  VQAPFSCSDPISAHKFIRFRKMMRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120

Query: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 180
           TTEVS RHMKPSWQLQYTLTDAPMAYDG+ Y+ELL TFKSI GFFLIVGEAGIGKSTLAK
Sbjct: 121 TTEVSVRHMKPSWQLQYTLTDAPMAYDGDPYAELLTTFKSIHGFFLIVGEAGIGKSTLAK 180

Query: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240
           FLY+ +EVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK
Sbjct: 181 FLYHHQEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240

Query: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG 300
           GTSFLLVIQDLSTTNLDDS+ L++LLGMGN         +NSKILVTTQSE TAGALGL 
Sbjct: 241 GTSFLLVIQDLSTTNLDDSNTLMKLLGMGN---------ENSKILVTTQSEMTAGALGLR 300

Query: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 334
            VYASEKQSEKQSTQNSDDKNQKVST FKLERL
Sbjct: 301 TVYASEKQSEKQSTQNSDDKNQKVSTGFKLERL 324

[Close]

BLAST of Carg03523 vs. NCBI nr
Match: XP_023528962.1 (putative disease resistance protein RGA4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 62.4 bits (150), Expect = 9.8e-06
Identity = 32/46 (69.57%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 334 LQAFTGVETAKIVGSVKVKAAAPGYGFQYWNTVIRGSSGSGSRKYY 380
           LQAF GVETAKI+GSVKV+   PGYGFQY NTVIR   G  S+K Y
Sbjct: 748 LQAFEGVETAKILGSVKVRETPPGYGFQYKNTVIRVPRGIDSKKEY 793

HSP 2 Score: 515.8 bits (1327), Expect = 3.2e-142
Identity = 275/330 (83.33%), Postives = 289/330 (87.58%), Query Frame = 0


Query: 5   MLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGKKVQA 64
           MLSLKSILFDAEEKQEQEQNHDLR WLKELQN FYQIED+MDEFKWD FEQ GTGKKVQA
Sbjct: 1   MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 60

Query: 65  PFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 124
           PF CS PISAHK K  RKM RL NHL+TLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE
Sbjct: 61  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 120

Query: 125 VSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAKFLY 184
           VS RHMKPSWQLQYTL DAP  Y  NRY++LL+TFKS  GFFLIVGEAGIGKSTLAKFLY
Sbjct: 121 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 180

Query: 185 NDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTS 244
           + K VVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL+KKQLHLKV+QLLKGTS
Sbjct: 181 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 240

Query: 245 FLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLGIVY 304
           FLLVIQDLSTTNLDDSSAL+ELL MGN         ++SKILVTTQSEETA ALG+ IVY
Sbjct: 241 FLLVIQDLSTTNLDDSSALMELLDMGN---------EDSKILVTTQSEETADALGVRIVY 300

Query: 305 ASEKQSEKQSTQNSDDKNQKVSTVFKLERL 334
           A     EKQS QNS+DKNQ+V T FKLERL
Sbjct: 301 A----FEKQSMQNSNDKNQEVPTGFKLERL 317

[Close]

BLAST of Carg03523 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 6.5e-21
Identity = 73/279 (26.16%), Postives = 136/279 (48.75%), Query Frame = 0


Query: 7   SLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKKVQAPFS 66
           +++++L DA+EK  Q  N  L  WL++L    Y+++D +DE+K         +  Q+ + 
Sbjct: 40  TIQAVLEDAQEK--QLNNKPLENWLQKLNAATYEVDDILDEYK-----TKATRFSQSEYG 99

Query: 67  CSHP-ISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVS 126
             HP +   + K  ++M ++   L  +A     FHL EK  +     +   S L+  +V 
Sbjct: 100 RYHPKVIPFRHKVGKRMDQVMKKLKAIAEERKNFHLHEKIVERQAVRRETGSVLTEPQVY 159

Query: 127 ARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAKFLYND 186
            R  +    ++  + +     D    S L            I+G  G+GK+TLA+ ++ND
Sbjct: 160 GRDKEKDEIVKILINNVS---DAQHLSVL-----------PILGMGGLGKTTLAQMVFND 219

Query: 187 KEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTSFL 246
           + V E + S+ W+CV E FD +RLI+ +   +  + +  ++    L  K+Q+LL G  +L
Sbjct: 220 QRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGRPLLGEMDLAPLQKKLQELLNGKRYL 279

Query: 247 LVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKI 285
           LV+ D+   +    + L  +L +G   + +  TT+  K+
Sbjct: 280 LVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKV 297

[Close]

BLAST of Carg03523 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 4.7e-19
Identity = 69/279 (24.73%), Postives = 141/279 (50.54%), Query Frame = 0


Query: 7   SLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKKVQAPFS 66
           +++++L DA+EKQ  ++   L  WL++L    Y+++D +DE+K         + +Q+ + 
Sbjct: 40  TIQAVLEDAQEKQLNDK--PLENWLQKLNAATYEVDDILDEYK-----TKATRFLQSEYG 99

Query: 67  CSHP-ISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVS 126
             HP +   + K  ++M ++   L+ +A    +FHL EK  +     +   S L+  +V 
Sbjct: 100 RYHPKVIPFRHKVGKRMDQVMKKLNAIAEERKKFHLQEKIIERQAATRETGSVLTEPQVY 159

Query: 127 ARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAKFLYND 186
            R  +    ++  +  A    D  + S L            I+G  G+GK+TL++ ++ND
Sbjct: 160 GRDKEKDEIVKILINTAS---DAQKLSVL-----------PILGMGGLGKTTLSQMVFND 219

Query: 187 KEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTSFL 246
           + V E++  + W+C+ + F+ +RLI+ +   +  K + +D+    L  K+Q+LL G  + 
Sbjct: 220 QRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSL-SDMDLAPLQKKLQELLNGKRYF 279

Query: 247 LVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKI 285
           LV+ D+   +    + L  +L +G   + +  TT+  K+
Sbjct: 280 LVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKV 296

[Close]

BLAST of Carg03523 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 6.8e-18
Identity = 71/278 (25.54%), Postives = 135/278 (48.56%), Query Frame = 0


Query: 8   LKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKKVQAPFSC 67
           ++++L DA+EKQ + +   ++ WL++L    Y+++D +D+ K +       +  QA    
Sbjct: 41  IQAVLEDAQEKQLKYK--AIKNWLQKLNVAAYEVDDILDDCKTE-----AARFKQAVLGR 100

Query: 68  SHPIS-AHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSA 127
            HP +    +K  ++M  +   LD +A     FHL E+  +     +     L+  +V  
Sbjct: 101 YHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLDERIIERQAARRQTGFVLTEPKVYG 160

Query: 128 RHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAKFLYNDK 187
           R  +    ++  + +         YSE +           I+G  G+GK+TLA+ ++ND+
Sbjct: 161 REKEEDEIVKILINNV-------SYSEEVPVLP-------ILGMGGLGKTTLAQMVFNDQ 220

Query: 188 EVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTSFLL 247
            + E +  + WVCV + FD +RLI+ +   +  K +  D+    L  K+Q+LL G  + L
Sbjct: 221 RITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSL-GDMDLAPLQKKLQELLNGKRYFL 280

Query: 248 VIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKI 285
           V+ D+   + +    L  +L +G   + I +TT+  KI
Sbjct: 281 VLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKI 296

[Close]

BLAST of Carg03523 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.4e-17
Identity = 76/285 (26.67%), Postives = 139/285 (48.77%), Query Frame = 0


Query: 7   SLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD---FEQEGTGKKVQA 66
           +++++L DA+EKQ +++   +  WL++L +  Y+++D + E K +   FEQ   G     
Sbjct: 40  TIQAVLQDAQEKQLKDK--AIENWLQKLNSAAYEVDDILGECKNEAIRFEQSRLG----- 99

Query: 67  PFSCSHP-ISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTT 126
                HP I   + K  R+M  +   LD ++    +FH  EK  +      T  +    T
Sbjct: 100 ---FYHPGIINFRHKIGRRMKEIMEKLDAISEERRKFHFLEKITERQAAAATRETGFVLT 159

Query: 127 E--VSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 186
           E  V  R  +    ++  + +  +A +                 F I+G  G+GK+TLA+
Sbjct: 160 EPKVYGRDKEEDEIVKILINNVNVAEE--------------LPVFPIIGMGGLGKTTLAQ 219

Query: 187 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVY-SVVACKEICNDLTKKQLHLKVQQLL 246
            ++ND+ V + +  + WVCV + FD +RLI+ +  ++        DL   Q   K+Q+LL
Sbjct: 220 MIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQ--KKLQELL 279

Query: 247 KGTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKI 285
            G  +LLV+ D+   +L+  + L  +L +G + + I  TT+  K+
Sbjct: 280 NGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRLEKV 298

[Close]

BLAST of Carg03523 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 9.2e-15
Identity = 83/301 (27.57%), Postives = 137/301 (45.51%), Query Frame = 0


Query: 5   MLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKKVQAP 64
           +L++ ++L DAEEK  Q  N  + KW+ EL++  Y  ED +D    D   E     + A 
Sbjct: 46  LLTITAVLIDAEEK--QITNPVVEKWVNELRDVVYHAEDALD----DIATEALRLNIGAE 105

Query: 65  FSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEV 124
            S     S+++ ++ R    L + LD  +  + E  L EK    + R+ +  + L   E+
Sbjct: 106 SS-----SSNRLRQLRGRMSLGDFLDGNSEHL-ETRL-EKVTIRLERLASQRNILGLKEL 165

Query: 125 SARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRG------FFLIVGEAGIGKSTL 184
           +A   K        + ++ +    +   E++R      G         IVG  G+GK+TL
Sbjct: 166 TAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTL 225

Query: 185 AKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQL 244
           ++ LYND+ V   + ++ W  V E FD  ++ ++VY  V  +  C       L +K+++ 
Sbjct: 226 SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRP-CEFTDLDVLQVKLKER 285

Query: 245 LKGTS--FLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGA 298
           L GT   FLLV+ DL   N  D   L                 Q S+ILVTT+S+  A  
Sbjct: 286 LTGTGLPFLLVLDDLWNENFADWDLL---------RQPFIHAAQGSQILVTTRSQRVASI 323

[Close]

BLAST of Carg03523 vs. ExPASy TrEMBL
Match: A0A6J1F328 (disease resistance protein RGA2-like OS=Cucurbita moschata OX=3662 GN=LOC111441733 PE=4 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 1.1e-169
Identity = 312/333 (93.69%), Postives = 315/333 (94.59%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60
           MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK
Sbjct: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60

Query: 61  VQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLS 120
           VQAPFSCSHPISAHKF RFRKM RLRNHLDTLAARMYEFHLTEKHPDSIGR KTAHSFLS
Sbjct: 61  VQAPFSCSHPISAHKFIRFRKMMRLRNHLDTLAARMYEFHLTEKHPDSIGRRKTAHSFLS 120

Query: 121 TTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAK 180
           TTEVS RHMKPSWQLQYTLTDAPM YDG+RY+ELL TFKSI GFFLIVGEAGIGKSTLAK
Sbjct: 121 TTEVSVRHMKPSWQLQYTLTDAPMTYDGDRYAELLTTFKSIHGFFLIVGEAGIGKSTLAK 180

Query: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLK 240
           FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQ LLK
Sbjct: 181 FLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQHLLK 240

Query: 241 GTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLG 300
           GTSFLLVIQDLSTTNLDDSS L+ELLGMGNK         NSKILVTTQSEETAGALGLG
Sbjct: 241 GTSFLLVIQDLSTTNLDDSSTLMELLGMGNK---------NSKILVTTQSEETAGALGLG 300

Query: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 334
           IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL
Sbjct: 301 IVYASEKQSEKQSTQNSDDKNQKVSTVFKLERL 324

[Close]

BLAST of Carg03523 vs. ExPASy TrEMBL
Match: A0A6J1F328 (disease resistance protein RGA2-like OS=Cucurbita moschata OX=3662 GN=LOC111441733 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.5e-15
Identity = 45/46 (97.83%), Postives = 45/46 (97.83%), Query Frame = 0


Query: 334 LQAFTGVETAKIVGSVKVKAAAPGYGFQYWNTVIRGSSGSGSRKYY 380
           LQAFTGVETAKIVGSVKVK AAPGYGFQYWNTVIRGSSGSGSRKYY
Sbjct: 748 LQAFTGVETAKIVGSVKVKGAAPGYGFQYWNTVIRGSSGSGSRKYY 793

HSP 2 Score: 515.8 bits (1327), Expect = 1.6e-142
Identity = 275/330 (83.33%), Postives = 289/330 (87.58%), Query Frame = 0


Query: 5   MLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGKKVQA 64
           MLSLKSILFDAEEKQEQEQNHDLR WLKELQN FYQIED+MDEFKWD FEQ GTGKKVQA
Sbjct: 1   MLSLKSILFDAEEKQEQEQNHDLRNWLKELQNIFYQIEDSMDEFKWDFFEQGGTGKKVQA 60

Query: 65  PFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 124
           PF CS PISAHK K  RKM RL NHL+TLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE
Sbjct: 61  PFLCSDPISAHKSKLIRKMKRLCNHLNTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTE 120

Query: 125 VSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLAKFLY 184
           VS RHMKPSWQLQYTL DAP  Y  NRY++LL+TFKS  GFFLIVGEAGIGKSTLAKFLY
Sbjct: 121 VSVRHMKPSWQLQYTLVDAPTDYHDNRYADLLQTFKSSHGFFLIVGEAGIGKSTLAKFLY 180

Query: 185 NDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTS 244
           + K VVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDL+KKQLHLKV+QLLKGTS
Sbjct: 181 HHKVVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLSKKQLHLKVRQLLKGTS 240

Query: 245 FLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGALGLGIVY 304
           FLLVIQDLSTTNLDDSSAL+ELL MGN         ++SKILVTTQSEETA ALG+ IVY
Sbjct: 241 FLLVIQDLSTTNLDDSSALMELLDMGN---------EDSKILVTTQSEETADALGVRIVY 300

Query: 305 ASEKQSEKQSTQNSDDKNQKVSTVFKLERL 334
           A     EKQS QNS+DKNQ+V T FKLERL
Sbjct: 301 A----FEKQSMQNSNDKNQEVPTGFKLERL 317

[Close]

BLAST of Carg03523 vs. ExPASy TrEMBL
Match: A0A6J1J5Q0 (putative disease resistance protein RGA1 OS=Cucurbita maxima OX=3661 GN=LOC111481987 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.4e-12
Identity = 41/46 (89.13%), Postives = 43/46 (93.48%), Query Frame = 0


Query: 334 LQAFTGVETAKIVGSVKVKAAAPGYGFQYWNTVIRGSSGSGSRKYY 380
           LQAF GVETAKI+GSVKVK AAPGYGFQY NTVIRGSSGSGSRKY+
Sbjct: 741 LQAFKGVETAKILGSVKVKEAAPGYGFQYRNTVIRGSSGSGSRKYF 786

HSP 2 Score: 325.1 bits (832), Expect = 3.9e-85
Identity = 188/314 (59.87%), Postives = 228/314 (72.61%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKK 60
           MEYTMLSLK+IL DAEEK  QEQNH LR WLKELQ+ FYQ ED++DE KW  EQEG G+K
Sbjct: 66  MEYTMLSLKAILLDAEEK--QEQNHGLRNWLKELQDVFYQTEDSIDELKW--EQEGIGRK 125

Query: 61  VQAPFSCSHPISAH-KFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFL 120
           V+A FSCS   +AH K     KM +L +HLD +A++MYEFHLTE+H  SI RM+TAHSFL
Sbjct: 126 VRARFSCSDKTAAHKKTNPTSKMKKLCDHLDEIASKMYEFHLTEEHTSSI-RMETAHSFL 185

Query: 121 STTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTF-KSIRGFFLIVGEAGIGKSTL 180
           S TEVS RHM+PSWQL Y LTDAP +Y  +RY ELL  F  S + FF IVGEAGIGKSTL
Sbjct: 186 SATEVSTRHMRPSWQLFYPLTDAPNSYCNSRYVELLNDFIHSKQQFFHIVGEAGIGKSTL 245

Query: 181 AKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQL 240
           AKF Y+  +V   + SR WVCVK+G++T+RL++EVYS    KE+ +D TKKQL LKVQ+ 
Sbjct: 246 AKFFYSHHDVCSSFTSRSWVCVKDGYNTKRLLKEVYSFQDDKEVRDDFTKKQLLLKVQRF 305

Query: 241 LKGTSFLLVIQDLSTTNL-DDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGAL 300
           L+G +FL+V QDLS+ NL DD S L  LL +GN           SKI+VTTQ+EE A  L
Sbjct: 306 LRGQTFLVVFQDLSSKNLEDDRSMLKNLLELGN---------YGSKIIVTTQNEEIADDL 365

Query: 301 GLGIVYASEKQSEK 312
            L  VY ++K SE+
Sbjct: 366 KLKKVYKTKKLSEQ 365

[Close]

BLAST of Carg03523 vs. ExPASy TrEMBL
Match: A0A0A0LBH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G815400 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 5.7e-76
Identity = 174/325 (53.54%), Postives = 217/325 (66.77%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGK 60
           +EY M SLK++L DAEEKQEQ Q   L+ WLKELQN FYQ+ED++DEFKW+ F+Q+  GK
Sbjct: 26  IEYAMSSLKAVLLDAEEKQEQNQR--LQNWLKELQNVFYQVEDSIDEFKWEIFKQKDIGK 85

Query: 61  KVQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFL 120
           +V APFSCS+ ISA+K K+  K  ++ + L+ +A RMYEFHL  KH DSI  M+T H+F 
Sbjct: 86  QVLAPFSCSNQISANKLKQKCKRKQVCDELNNIATRMYEFHLKVKHIDSIS-METTHTFP 145

Query: 121 STTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLIVGEAGIGKSTLA 180
           S +E+S RH+KPSWQL Y L DA   YD   Y  +L  F      F IVGEAGIGKST+A
Sbjct: 146 SASEISIRHLKPSWQLLYPLIDASRTYD-EIYDGILNVFNECTHVFHIVGEAGIGKSTVA 205

Query: 181 KFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLL 240
           +FLYN   VV +Y SR+WVCV+EGF+  RL++EVYS    KEIC DLT +QL  KV++LL
Sbjct: 206 RFLYNHNNVVGKYTSRYWVCVEEGFNKHRLVKEVYSHADNKEICEDLTTEQLLSKVKRLL 265

Query: 241 KGTSFLLVIQDLSTTNLDDSSALV--ELLGMGNKNSKISVTTQNSKILVTTQ------SE 300
           K  +FLLV QDLS TNL D S LV  ELL MG   SKI VTTQ  +I    Q        
Sbjct: 266 KVETFLLVFQDLSITNLKDRSPLVLNELLEMGQHGSKIIVTTQTEEIANYLQDRGYKTER 325

Query: 301 ETAGALGLGIVYASEKQSEKQSTQN 317
            +   L  G   ++  QS  ++TQN
Sbjct: 326 RSKENLANGDRVSAHNQSLTKNTQN 346

[Close]

BLAST of Carg03523 vs. ExPASy TrEMBL
Match: A0A1S3B6X8 (putative disease resistance protein RGA1 OS=Cucumis melo OX=3656 GN=LOC103486832 PE=4 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 4.5e-73
Identity = 176/353 (49.86%), Postives = 229/353 (64.87%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-FEQEGTGK 60
           +EYTM SLK+IL DAEEKQEQ Q   L  WLKELQN FYQ+ED++DEFKW+ F+Q+ TGK
Sbjct: 26  IEYTMSSLKAILLDAEEKQEQNQR--LHDWLKELQNVFYQVEDSIDEFKWEFFKQKDTGK 85

Query: 61  KVQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFL 120
           +V A FSCS  ISA+K K+  K  ++ + L+ +AA MYEFHL  KH  SI  M+T HSF 
Sbjct: 86  QVLAHFSCSSRISANKLKQNFKRNKVCDELNKIAANMYEFHLKVKHIGSIS-METTHSFP 145

Query: 121 STTEVSARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTF-KSIRGFFLIVGEAGIGKSTL 180
           S +E+S RH+KPSWQL Y L  A   YD   Y  +L  F K   GFF IVGEAGIGKST+
Sbjct: 146 SASEISTRHLKPSWQLLYPLKHASRNYD-EAYDGILNVFNKYTHGFFHIVGEAGIGKSTM 205

Query: 181 AKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQL 240
           A+FLYN + VV++Y  R+WVCV+EGF+T RL++E+YS    KE   DLT +QL  K  +L
Sbjct: 206 ARFLYNHENVVDRYTRRYWVCVEEGFNTHRLMKEIYSHADNKETREDLTTEQLLSKFIRL 265

Query: 241 LKGTSFLLVIQDLSTTNLD--DSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGA 300
            +  +FLLV QDLS TNLD   +S L++LL MG+          +SKI+VTTQ+EE A  
Sbjct: 266 QREETFLLVFQDLSITNLDKCSTSVLIKLLEMGH---------HDSKIIVTTQTEEIAKD 325

Query: 301 LGLGIVYASEKQSEKQSTQNSDDKNQKVSTVFKLERLLQAFTGVETAKIVGSV 350
           + +   Y +E QS+K      +    K S     +   QA  G +T +  G++
Sbjct: 326 IDVH-GYKTEIQSKKNLANGDEVSADKQSLTKNTQN--QAVPGSQTEETAGAI 362

[Close]

BLAST of Carg03523 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 82.8 bits (203), Expect = 6.5e-16
Identity = 83/301 (27.57%), Postives = 137/301 (45.51%), Query Frame = 0


Query: 5   MLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWDFEQEGTGKKVQAP 64
           +L++ ++L DAEEK  Q  N  + KW+ EL++  Y  ED +D    D   E     + A 
Sbjct: 46  LLTITAVLIDAEEK--QITNPVVEKWVNELRDVVYHAEDALD----DIATEALRLNIGAE 105

Query: 65  FSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEV 124
            S     S+++ ++ R    L + LD  +  + E  L EK    + R+ +  + L   E+
Sbjct: 106 SS-----SSNRLRQLRGRMSLGDFLDGNSEHL-ETRL-EKVTIRLERLASQRNILGLKEL 165

Query: 125 SARHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRG------FFLIVGEAGIGKSTL 184
           +A   K        + ++ +    +   E++R      G         IVG  G+GK+TL
Sbjct: 166 TAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNGITVVAIVGIGGVGKTTL 225

Query: 185 AKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQL 244
           ++ LYND+ V   + ++ W  V E FD  ++ ++VY  V  +  C       L +K+++ 
Sbjct: 226 SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRP-CEFTDLDVLQVKLKER 285

Query: 245 LKGTS--FLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETAGA 298
           L GT   FLLV+ DL   N  D   L                 Q S+ILVTT+S+  A  
Sbjct: 286 LTGTGLPFLLVLDDLWNENFADWDLL---------RQPFIHAAQGSQILVTTRSQRVASI 323

[Close]

BLAST of Carg03523 vs. TAIR 10
Match: AT3G50950.2 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 61.2 bits (147), Expect = 2.0e-09
Identity = 67/291 (23.02%), Postives = 128/291 (43.99%), Query Frame = 0


Query: 8   LKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKW-DFEQEGTGKKVQAPFS 67
           ++S L DAE  +++  N  LR  + +L+   Y+ ED + + +  D +     +   A  S
Sbjct: 41  MQSFLKDAE--RQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWLS 100

Query: 68  CSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSA 127
             HP  A    +++K  RL+              + E+      +++    F++ + V  
Sbjct: 101 RLHP--ARVPLQYKKSKRLQ-------------EINERITKIKSQVEPYFEFITPSNVGR 160

Query: 128 RHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLI---VGEAGIGKSTLAKFLY 187
            +    W          +  +G++       F+S     LI   VG  G+GK+T+A+ ++
Sbjct: 161 DNGTDRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGMGGLGKTTIAQEVF 220

Query: 188 NDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTS 247
           NDKE+  ++  R WV V + F  ++++  +   +    + +D+    L  K+QQ L G  
Sbjct: 221 NDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDI--GTLLRKIQQYLLGKR 280

Query: 248 FLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETA 295
           +L+V+ D+   NL     + + L  G          Q   ++VTT+SE  A
Sbjct: 281 YLIVMDDVWDKNLSWWDKIYQGLPRG----------QGGSVIVTTRSESVA 302

[Close]

BLAST of Carg03523 vs. TAIR 10
Match: AT3G50950.1 (HOPZ-ACTIVATED RESISTANCE 1 )

HSP 1 Score: 61.2 bits (147), Expect = 2.0e-09
Identity = 67/291 (23.02%), Postives = 128/291 (43.99%), Query Frame = 0


Query: 8   LKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKW-DFEQEGTGKKVQAPFS 67
           ++S L DAE  +++  N  LR  + +L+   Y+ ED + + +  D +     +   A  S
Sbjct: 41  MQSFLKDAE--RQKRTNETLRTLVADLRELVYEAEDILVDCQLADGDDGNEQRSSNAWLS 100

Query: 68  CSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMKTAHSFLSTTEVSA 127
             HP  A    +++K  RL+              + E+      +++    F++ + V  
Sbjct: 101 RLHP--ARVPLQYKKSKRLQ-------------EINERITKIKSQVEPYFEFITPSNVGR 160

Query: 128 RHMKPSWQLQYTLTDAPMAYDGNRYSELLRTFKSIRGFFLI---VGEAGIGKSTLAKFLY 187
            +    W          +  +G++       F+S     LI   VG  G+GK+T+A+ ++
Sbjct: 161 DNGTDRWSSPVYDHTQVVGLEGDKRKIKEWLFRSNDSQLLIMAFVGMGGLGKTTIAQEVF 220

Query: 188 NDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICNDLTKKQLHLKVQQLLKGTS 247
           NDKE+  ++  R WV V + F  ++++  +   +    + +D+    L  K+QQ L G  
Sbjct: 221 NDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGDDI--GTLLRKIQQYLLGKR 280

Query: 248 FLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSKILVTTQSEETA 295
           +L+V+ D+   NL     + + L  G          Q   ++VTT+SE  A
Sbjct: 281 YLIVMDDVWDKNLSWWDKIYQGLPRG----------QGGSVIVTTRSESVA 302

[Close]

BLAST of Carg03523 vs. TAIR 10
Match: AT1G58390.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 56.2 bits (134), Expect = 6.5e-08
Identity = 35/123 (28.46%), Postives = 68/123 (55.28%), Query Frame = 0


Query: 167 IVGEAGIGKSTLAKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKEICND- 226
           I G  G+GK+TLA+ ++N ++V  Q+    WVCV + F  + + + +   +  +E  ++ 
Sbjct: 188 ITGMGGLGKTTLARQVFNHEDVKHQFDRLAWVCVSQEFTRKNVWQMILQNLTSREKKDEI 247

Query: 227 --LTKKQLHLKVQQLLKGTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQNSK 286
             + + +LH K+ QLL+ +  L+V  D+     D+   L++ +   NK  K+ +T+QN  
Sbjct: 248 LQMEEAELHDKLFQLLETSKSLIVFDDIWK---DEDWDLIKPIFPPNKGWKVLLTSQNES 307

[Close]

BLAST of Carg03523 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-07
Identity = 64/312 (20.51%), Postives = 126/312 (40.38%), Query Frame = 0


Query: 1   MEYTMLSLKSILFDAEEKQEQEQNHDLRKWLKELQNTFYQIEDTMDEFKWD-------FE 60
           ++  +++   +L DA+++ E  +  +++ WL  +++ F+Q ED +DE + +        E
Sbjct: 39  LKVALVTANPVLADADQRAEHVR--EVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAE 98

Query: 61  QEGTGKKVQAPFSCSHPISAHKFKRFRKMTRLRNHLDTLAARMYEFHLTEKHPDSIGRMK 120
             G G   Q   +    I      +  K+ RL  H           H+  KH + IG  +
Sbjct: 99  AGGLGGLFQNLMAGREAIQKKIEPKMEKVVRLLEH-----------HV--KHIEVIGLKE 158

Query: 121 TAHSFLSTTEVSARHMKPSWQ--LQYTLTDAPMAYDGNRYSELLRTFKSI---------- 180
            + +            +P W+   +    D P      R  + L     +          
Sbjct: 159 YSET-----------REPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGK 218

Query: 181 RGFFLIVGEAGIGKSTLAKFLYNDKEVVEQYLSRFWVCVKEGFDTQRLIEEVYSVVACKE 240
                +VG  G+GK+TL + ++ND  V E +  + W+     F+   + + V   +    
Sbjct: 219 PAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSA 278

Query: 241 I-CNDLTKKQLHLKVQQLLKGTSFLLVIQDLSTTNLDDSSALVELLGMGNKNSKISVTTQ 293
           +   DL   Q+ LK  + L G  FLLV+ D  + +  +  +         + SKI +TT+
Sbjct: 279 VNTEDLPSLQIQLK--KTLSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTR 322

The following BLAST results are available for this feature:

BLAST of Carg03523 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017603.1	3.6e-210	100.00	Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. argyrosp...	[more]
KAG6580847.1	4.2e-182	99.40	Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6580847.1	5.2e-15	97.83	Disease resistance protein RGA2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022934599.1	5.2e-15	97.83	disease resistance protein RGA2-like [Cucurbita moschata]	[more]
XP_023528962.1	9.8e-06	69.57	putative disease resistance protein RGA4 [Cucurbita pepo subsp. pepo]	[more]

Pages

BLAST of Carg03523 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XBQ9	6.5e-21	26.16	Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 ...	[more]
Q7XA42	4.7e-19	24.73	Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA40	6.8e-18	25.54	Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q7XA39	4.4e-17	26.67	Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=R...	[more]
Q9LRR4	9.2e-15	27.57	Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Carg03523 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F328	1.1e-169	93.69	disease resistance protein RGA2-like OS=Cucurbita moschata OX=3662 GN=LOC1114417...	[more]
A0A6J1F328	2.5e-15	97.83	disease resistance protein RGA2-like OS=Cucurbita moschata OX=3662 GN=LOC1114417...	[more]
A0A6J1J5Q0	4.4e-12	89.13	putative disease resistance protein RGA1 OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A0A0LBH9	5.7e-76	53.54	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G815400 PE=4 SV=1	[more]
A0A1S3B6X8	4.5e-73	49.86	putative disease resistance protein RGA1 OS=Cucumis melo OX=3656 GN=LOC103486832...	[more]

BLAST of Carg03523 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14470.1	6.5e-16	27.57	NB-ARC domain-containing disease resistance protein	[more]
AT3G50950.2	2.0e-09	23.02	HOPZ-ACTIVATED RESISTANCE 1	[more]
AT3G50950.1	2.0e-09	23.02	HOPZ-ACTIVATED RESISTANCE 1	[more]
AT1G58390.1	6.5e-08	28.46	Disease resistance protein (CC-NBS-LRR class) family	[more]
AT3G14460.1	4.2e-07	20.51	LRR and NB-ARC domains-containing disease resistance protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.5.4130		coord: 1..96 e-value: 1.4E-10 score: 43.2
None	No IPR available	PANTHER	PTHR23155:SF941	OS06G0268500 PROTEIN	coord: 147..289
IPR002182	NB-ARC	PFAM	PF00931	NB-ARC	coord: 164..286 e-value: 4.1E-14 score: 52.4
IPR041118	Rx, N-terminal	PFAM	PF18052	Rx_N	coord: 4..56 e-value: 9.0E-10 score: 38.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 147..299 e-value: 9.1E-20 score: 72.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 153..313
IPR044974	Disease resistance protein, plants	PANTHER	PTHR23155	DISEASE RESISTANCE PROTEIN RP	coord: 147..289

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03523-RA	Carg03523-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
molecular_function	GO:0043531	ADP binding