Carg03460 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03460
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionethylene-responsive transcription factor 3-like
LocationCarg_Chr20: 1191194 .. 1191853 (+)
RNA-Seq ExpressionCarg03460
SyntenyCarg03460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGCGAGGCAGACCCTCAGATCCATTTACAGCTACAGAACTACCAAAATTGACAGTTCATCCTGAAAATCCTGTCAAAGAGATACGATACAGAGGCGTTCGGAAGCGTCCTTGGGGCAGATACGCCGCTGAGATCAGAGATCCCTGGAAGAAAACTAGGGTTTGGCTCGGTACCTTTGATTCAGCTGAAGAGGCTGCTCGGGCCTACGATAATGCTGCTCGGACCCTCCGTGGAGCCAAGGCCAAAACTAACTTCCCTCTACATCCATCCACGATTTCCATTCAACAACAAATTCCGTTCTACCAATCGCCGCCAGATTTGCACGATTCGAACGGTTATGGTGCCAAGATTGAGTGTGTTCAGGCTAATAGGCCTACGTCGAGCAGTTTGAGTTCCACCGTGGAGTCGTTTAGTGGACCGCGGATTTCTAACCCTTTGTCGTCGCTGTCTATGGTTCGCCGGAGGCCGGTGCAGCCAGTTTCGCCGGACGACTGCCATAGCGACTGTGATTCGTCCTCCTCGGTTGTTGATGATGATGAGGTCTGTGTGCTCGCTTCTTTCCGTCGAGCCTTGCCCTTCGATCTTAACTTACCGCCTCCCATGGACGTCGTGGATTTAACAGGTGACGATCTTCAAGCTACTGCTTTATGCCTCTGA

mRNA sequence

ATGCGGCGAGGCAGACCCTCAGATCCATTTACAGCTACAGAACTACCAAAATTGACAGTTCATCCTGAAAATCCTGTCAAAGAGATACGATACAGAGGCGTTCGGAAGCGTCCTTGGGGCAGATACGCCGCTGAGATCAGAGATCCCTGGAAGAAAACTAGGGTTTGGCTCGGTACCTTTGATTCAGCTGAAGAGGCTGCTCGGGCCTACGATAATGCTGCTCGGACCCTCCGTGGAGCCAAGGCCAAAACTAACTTCCCTCTACATCCATCCACGATTTCCATTCAACAACAAATTCCGTTCTACCAATCGCCGCCAGATTTGCACGATTCGAACGGTTATGGTGCCAAGATTGAGTGTGTTCAGGCTAATAGGCCTACGTCGAGCAGTTTGAGTTCCACCGTGGAGTCGTTTAGTGGACCGCGGATTTCTAACCCTTTGTCGTCGCTGTCTATGGTTCGCCGGAGGCCGGTGCAGCCAGTTTCGCCGGACGACTGCCATAGCGACTGTGATTCGTCCTCCTCGGTTGTTGATGATGATGAGGTCTGTGTGCTCGCTTCTTTCCGTCGAGCCTTGCCCTTCGATCTTAACTTACCGCCTCCCATGGACGTCGTGGATTTAACAGGTGACGATCTTCAAGCTACTGCTTTATGCCTCTGA

Coding sequence (CDS)

ATGCGGCGAGGCAGACCCTCAGATCCATTTACAGCTACAGAACTACCAAAATTGACAGTTCATCCTGAAAATCCTGTCAAAGAGATACGATACAGAGGCGTTCGGAAGCGTCCTTGGGGCAGATACGCCGCTGAGATCAGAGATCCCTGGAAGAAAACTAGGGTTTGGCTCGGTACCTTTGATTCAGCTGAAGAGGCTGCTCGGGCCTACGATAATGCTGCTCGGACCCTCCGTGGAGCCAAGGCCAAAACTAACTTCCCTCTACATCCATCCACGATTTCCATTCAACAACAAATTCCGTTCTACCAATCGCCGCCAGATTTGCACGATTCGAACGGTTATGGTGCCAAGATTGAGTGTGTTCAGGCTAATAGGCCTACGTCGAGCAGTTTGAGTTCCACCGTGGAGTCGTTTAGTGGACCGCGGATTTCTAACCCTTTGTCGTCGCTGTCTATGGTTCGCCGGAGGCCGGTGCAGCCAGTTTCGCCGGACGACTGCCATAGCGACTGTGATTCGTCCTCCTCGGTTGTTGATGATGATGAGGTCTGTGTGCTCGCTTCTTTCCGTCGAGCCTTGCCCTTCGATCTTAACTTACCGCCTCCCATGGACGTCGTGGATTTAACAGGTGACGATCTTCAAGCTACTGCTTTATGCCTCTGA

Protein sequence

MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIECVQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDDEVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Homology
BLAST of Carg03460 vs. NCBI nr
Match: XP_022944504.1 (ethylene-responsive transcription factor 3-like [Cucurbita moschata] >XP_022986032.1 ethylene-responsive transcription factor 3-like [Cucurbita maxima] >KAG6570551.1 Ethylene-responsive transcription factor 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010409.1 Ethylene-responsive transcription factor 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 446.8 bits (1148), Expect = 1.1e-121
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. NCBI nr
Match: XP_038902603.1 (ethylene-responsive transcription factor 3-like [Benincasa hispida])

HSP 1 Score: 436.0 bits (1120), Expect = 1.9e-118
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATE+PK T+ PENP+KEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATEIPKSTIQPENPLKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNG+G KIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGFGGKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. NCBI nr
Match: XP_004143195.1 (ethylene-responsive transcription factor 3 [Cucumis sativus] >XP_008456400.1 PREDICTED: ethylene-responsive transcription factor 3-like [Cucumis melo] >KAA0054487.1 ethylene-responsive transcription factor 3-like [Cucumis melo var. makuwa] >KGN47005.1 hypothetical protein Csa_020596 [Cucumis sativus] >TYK14552.1 ethylene-responsive transcription factor 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 435.6 bits (1119), Expect = 2.5e-118
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATE+P  T+ PENP+KEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATEIPNSTIQPENPLKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNG+GAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGFGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. NCBI nr
Match: XP_023512775.1 (ethylene-responsive transcription factor 3-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 426.8 bits (1096), Expect = 1.1e-115
Identity = 209/211 (99.05%), Postives = 210/211 (99.53%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNF LHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFSLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDD 212
           EVCVLASFRRALPFDLNLPPPMDVVDLTG+D
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGED 211

BLAST of Carg03460 vs. NCBI nr
Match: XP_023512776.1 (ethylene-responsive transcription factor 3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.0 bits (1081), Expect = 6.2e-114
Identity = 207/208 (99.52%), Postives = 207/208 (99.52%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNF LHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFSLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLT 209
           EVCVLASFRRALPFDLNLPPPMDVVDLT
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLT 208

BLAST of Carg03460 vs. ExPASy Swiss-Prot
Match: Q9SXS8 (Ethylene-responsive transcription factor 3 OS=Nicotiana tabacum OX=4097 GN=ERF3 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-51
Identity = 129/233 (55.36%), Postives = 146/233 (62.66%), Query Frame = 0

Query: 1   MRRGR-PSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGT 60
           MRRGR  + P   T  P    +     KEIR+RGVRKRPWGR+AAEIRDPWKKTRVWLGT
Sbjct: 1   MRRGRAAAAPAPVTGEP----NGSGGSKEIRFRGVRKRPWGRFAAEIRDPWKKTRVWLGT 60

Query: 61  FDSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIE 120
           FDSAE+AARAYD AAR LRG KAKTNFPL P     Q       +     DS  Y  +  
Sbjct: 61  FDSAEDAARAYDAAARALRGPKAKTNFPL-PYAHHHQFNQGHNPNNDPFVDSRFY-PQDN 120

Query: 121 CVQANRPTSSSLSSTVESFSGPR---ISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSV 180
            + + RPTSSS+SSTVESFSGPR        ++ S  +     PV PDDCHSDCDSSSSV
Sbjct: 121 PIISQRPTSSSMSSTVESFSGPRPPPAPRQQTTASSRKYTRSPPVVPDDCHSDCDSSSSV 180

Query: 181 VD----------DDEVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           VD          D++    +SFR+ L FDLNLPPPMD  D   DDL  TALCL
Sbjct: 181 VDHGDCEKENDNDNDNIASSSFRKPLLFDLNLPPPMD--DAGADDLHCTALCL 225

BLAST of Carg03460 vs. ExPASy Swiss-Prot
Match: O80339 (Ethylene-responsive transcription factor 3 OS=Arabidopsis thaliana OX=3702 GN=ERF3 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 8.6e-50
Identity = 127/233 (54.51%), Postives = 145/233 (62.23%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGR S   +A   P +       VKEIR+RGVRKRPWGR+AAEIRDPWKK RVWLGTF
Sbjct: 1   MRRGRGS---SAVAGPTVVAAINGSVKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPST----------ISIQQQIPFYQSPPDLHD 120
           DSAEEAARAYD+AAR LRG KAKTNFP+  S+          I  Q Q    Q  P +  
Sbjct: 61  DSAEEAARAYDSAARNLRGPKAKTNFPIDSSSPPPPNLRFNQIRNQNQ---NQVDPFMDH 120

Query: 121 SNGYGAKIECVQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDC 180
                 + +    NRPTSSS+SSTVESFSGPR    +   +  R     PV P+DCHSDC
Sbjct: 121 RLFTDHQQQFPIVNRPTSSSMSSTVESFSGPR-PTTMKPATTKRYPRTPPVVPEDCHSDC 180

Query: 181 DSSSSVVDDDEVCVLASFRRALP--FDLNLPPPMDVVDL--TGDDLQATALCL 220
           DSSSSV+DDD+    +S RR  P  FDLN  PP+D VDL    DDL  T L L
Sbjct: 181 DSSSSVIDDDDDIASSSRRRNPPFQFDLNF-PPLDCVDLFNGADDLHCTDLRL 225

BLAST of Carg03460 vs. ExPASy Swiss-Prot
Match: Q9LDE4 (Ethylene-responsive transcription factor 7 OS=Arabidopsis thaliana OX=3702 GN=ERF7 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.4e-46
Identity = 127/251 (50.60%), Postives = 149/251 (59.36%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MR+GR S    +   P L V     VKE RYRGVRKRPWGR+AAEIRDP KK+RVWLGTF
Sbjct: 1   MRKGRGS----SVVGPALPVTAGGSVKEPRYRGVRKRPWGRFAAEIRDPLKKSRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQ-----SPPDL------- 120
           DSA +AARAYD AAR LRG KAKTNFP+  S  S  Q + +       SPP +       
Sbjct: 61  DSAVDAARAYDTAARNLRGPKAKTNFPIDCSPSSPLQPLTYLHNQNLCSPPVIQNQIDPF 120

Query: 121 --HDSNGYGAKIECVQ---ANRPTSSSLSSTVESFSGPR----------ISNPLSSLSMV 180
             H   G G   E  Q    +RP SSS+SSTV+S SGPR          ++ PL ++   
Sbjct: 121 MDHRLYGGGNFQEQQQQQIISRPASSSMSSTVKSCSGPRPMEAAAASSSVAKPLHAIKRY 180

Query: 181 RRRPVQPVSPDDCHSDCDSSSSVVDDDEVCVLASFRRALP--FDLNLPPPMDVVDLTG-- 220
            R P  PV+P+DCHSDCDSSSSV+DD +    +S RR  P  FDLN  PP+D VDL    
Sbjct: 181 PRTP--PVAPEDCHSDCDSSSSVIDDGDDIASSSSRRKTPFQFDLNF-PPLDGVDLFAGG 240

BLAST of Carg03460 vs. ExPASy Swiss-Prot
Match: O80340 (Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ERF4 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.7e-27
Identity = 91/215 (42.33%), Postives = 101/215 (46.98%), Query Frame = 0

Query: 16  PKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDSAEEAARAYDNAAR 75
           P  T    N  KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTFD+AEEAARAYD AAR
Sbjct: 10  PATTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAR 69

Query: 76  TLRGAKAKTNFPLHPSTISIQQQIPFYQS-------------------------PPDLHD 135
             RGAKAKTNFP        +    F +S                         PP L  
Sbjct: 70  DFRGAKAKTNFPTFLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLEL 129

Query: 136 SNGYGAKIECVQ--ANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHS 195
           S G G    C Q   +RP        +          P    S  R   V   +   C +
Sbjct: 130 SLGGGGGGSCYQIPMSRPV---YFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMACGA 189

Query: 196 DCDSSSSVVDDDEVCVLASFRRALPFDLNLPPPMD 204
             DS SS V D E   +    + L  DLNLPPP +
Sbjct: 190 QSDSDSSSVVDFE-GGMEKRSQLLDLDLNLPPPSE 220

BLAST of Carg03460 vs. ExPASy Swiss-Prot
Match: Q9MAI5 (Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ERF8 PE=1 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 3.0e-26
Identity = 68/119 (57.14%), Postives = 74/119 (62.18%), Query Frame = 0

Query: 4   GRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDSA 63
           G   DP   T     T H  N  KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTFD+A
Sbjct: 7   GLKPDPVAPT---NPTHHESNAAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTA 66

Query: 64  EEAARAYDNAARTLRGAKAKTNF-------PLHPSTISIQQQIPFYQSPPDLHDSNGYG 116
           ++AARAYD AAR  RG KAKTNF       P   ST+        + +PP L  S G G
Sbjct: 67  QQAARAYDAAARDFRGVKAKTNFGVIVGSSPTQSSTVVDSPTAARFITPPHLELSLGGG 122

BLAST of Carg03460 vs. ExPASy TrEMBL
Match: A0A6J1J6H0 (ethylene-responsive transcription factor 3-like OS=Cucurbita maxima OX=3661 GN=LOC111483890 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.1e-122
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. ExPASy TrEMBL
Match: A0A6J1FWT6 (ethylene-responsive transcription factor 3-like OS=Cucurbita moschata OX=3662 GN=LOC111448942 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.1e-122
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. ExPASy TrEMBL
Match: A0A5A7UIU5 (Ethylene-responsive transcription factor 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold552G00350 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATE+P  T+ PENP+KEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATEIPNSTIQPENPLKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNG+GAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGFGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. ExPASy TrEMBL
Match: A0A0A0KBH0 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G167230 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATE+P  T+ PENP+KEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATEIPNSTIQPENPLKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNG+GAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGFGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. ExPASy TrEMBL
Match: A0A1S3C351 (ethylene-responsive transcription factor 3-like OS=Cucumis melo OX=3656 GN=LOC103496350 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 212/219 (96.80%), Postives = 216/219 (98.63%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGRPSDPFTATE+P  T+ PENP+KEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF
Sbjct: 1   MRRGRPSDPFTATEIPNSTIQPENPLKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGYGAKIEC 120
           DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNG+GAKIEC
Sbjct: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQSPPDLHDSNGFGAKIEC 120

Query: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180
           VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD
Sbjct: 121 VQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDCDSSSSVVDDD 180

Query: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 220
           EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL
Sbjct: 181 EVCVLASFRRALPFDLNLPPPMDVVDLTGDDLQATALCL 219

BLAST of Carg03460 vs. TAIR 10
Match: AT1G50640.1 (ethylene responsive element binding factor 3 )

HSP 1 Score: 198.4 bits (503), Expect = 6.1e-51
Identity = 127/233 (54.51%), Postives = 145/233 (62.23%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MRRGR S   +A   P +       VKEIR+RGVRKRPWGR+AAEIRDPWKK RVWLGTF
Sbjct: 1   MRRGRGS---SAVAGPTVVAAINGSVKEIRFRGVRKRPWGRFAAEIRDPWKKARVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPST----------ISIQQQIPFYQSPPDLHD 120
           DSAEEAARAYD+AAR LRG KAKTNFP+  S+          I  Q Q    Q  P +  
Sbjct: 61  DSAEEAARAYDSAARNLRGPKAKTNFPIDSSSPPPPNLRFNQIRNQNQ---NQVDPFMDH 120

Query: 121 SNGYGAKIECVQANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHSDC 180
                 + +    NRPTSSS+SSTVESFSGPR    +   +  R     PV P+DCHSDC
Sbjct: 121 RLFTDHQQQFPIVNRPTSSSMSSTVESFSGPR-PTTMKPATTKRYPRTPPVVPEDCHSDC 180

Query: 181 DSSSSVVDDDEVCVLASFRRALP--FDLNLPPPMDVVDL--TGDDLQATALCL 220
           DSSSSV+DDD+    +S RR  P  FDLN  PP+D VDL    DDL  T L L
Sbjct: 181 DSSSSVIDDDDDIASSSRRRNPPFQFDLNF-PPLDCVDLFNGADDLHCTDLRL 225

BLAST of Carg03460 vs. TAIR 10
Match: AT3G20310.1 (ethylene response factor 7 )

HSP 1 Score: 186.0 bits (471), Expect = 3.2e-47
Identity = 127/251 (50.60%), Postives = 149/251 (59.36%), Query Frame = 0

Query: 1   MRRGRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTF 60
           MR+GR S    +   P L V     VKE RYRGVRKRPWGR+AAEIRDP KK+RVWLGTF
Sbjct: 1   MRKGRGS----SVVGPALPVTAGGSVKEPRYRGVRKRPWGRFAAEIRDPLKKSRVWLGTF 60

Query: 61  DSAEEAARAYDNAARTLRGAKAKTNFPLHPSTISIQQQIPFYQ-----SPPDL------- 120
           DSA +AARAYD AAR LRG KAKTNFP+  S  S  Q + +       SPP +       
Sbjct: 61  DSAVDAARAYDTAARNLRGPKAKTNFPIDCSPSSPLQPLTYLHNQNLCSPPVIQNQIDPF 120

Query: 121 --HDSNGYGAKIECVQ---ANRPTSSSLSSTVESFSGPR----------ISNPLSSLSMV 180
             H   G G   E  Q    +RP SSS+SSTV+S SGPR          ++ PL ++   
Sbjct: 121 MDHRLYGGGNFQEQQQQQIISRPASSSMSSTVKSCSGPRPMEAAAASSSVAKPLHAIKRY 180

Query: 181 RRRPVQPVSPDDCHSDCDSSSSVVDDDEVCVLASFRRALP--FDLNLPPPMDVVDLTG-- 220
            R P  PV+P+DCHSDCDSSSSV+DD +    +S RR  P  FDLN  PP+D VDL    
Sbjct: 181 PRTP--PVAPEDCHSDCDSSSSVIDDGDDIASSSSRRKTPFQFDLNF-PPLDGVDLFAGG 240

BLAST of Carg03460 vs. TAIR 10
Match: AT3G15210.1 (ethylene responsive element binding factor 4 )

HSP 1 Score: 123.6 bits (309), Expect = 1.9e-28
Identity = 91/215 (42.33%), Postives = 101/215 (46.98%), Query Frame = 0

Query: 16  PKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDSAEEAARAYDNAAR 75
           P  T    N  KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTFD+AEEAARAYD AAR
Sbjct: 10  PATTNQTHNNAKEIRYRGVRKRPWGRYAAEIRDPGKKTRVWLGTFDTAEEAARAYDTAAR 69

Query: 76  TLRGAKAKTNFPLHPSTISIQQQIPFYQS-------------------------PPDLHD 135
             RGAKAKTNFP        +    F +S                         PP L  
Sbjct: 70  DFRGAKAKTNFPTFLELSDQKVPTGFARSPSQSSTLDCASPPTLVVPSATAGNVPPQLEL 129

Query: 136 SNGYGAKIECVQ--ANRPTSSSLSSTVESFSGPRISNPLSSLSMVRRRPVQPVSPDDCHS 195
           S G G    C Q   +RP        +          P    S  R   V   +   C +
Sbjct: 130 SLGGGGGGSCYQIPMSRPV---YFLDLMGIGNVGRGQPPPVTSAFRSPVVHVATKMACGA 189

Query: 196 DCDSSSSVVDDDEVCVLASFRRALPFDLNLPPPMD 204
             DS SS V D E   +    + L  DLNLPPP +
Sbjct: 190 QSDSDSSSVVDFE-GGMEKRSQLLDLDLNLPPPSE 220

BLAST of Carg03460 vs. TAIR 10
Match: AT1G53170.1 (ethylene response factor 8 )

HSP 1 Score: 120.2 bits (300), Expect = 2.1e-27
Identity = 68/119 (57.14%), Postives = 74/119 (62.18%), Query Frame = 0

Query: 4   GRPSDPFTATELPKLTVHPENPVKEIRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDSA 63
           G   DP   T     T H  N  KEIRYRGVRKRPWGRYAAEIRDP KKTRVWLGTFD+A
Sbjct: 7   GLKPDPVAPT---NPTHHESNAAKEIRYRGVRKRPWGRYAAEIRDPVKKTRVWLGTFDTA 66

Query: 64  EEAARAYDNAARTLRGAKAKTNF-------PLHPSTISIQQQIPFYQSPPDLHDSNGYG 116
           ++AARAYD AAR  RG KAKTNF       P   ST+        + +PP L  S G G
Sbjct: 67  QQAARAYDAAARDFRGVKAKTNFGVIVGSSPTQSSTVVDSPTAARFITPPHLELSLGGG 122

BLAST of Carg03460 vs. TAIR 10
Match: AT1G28370.1 (ERF domain protein 11 )

HSP 1 Score: 116.3 bits (290), Expect = 3.1e-26
Identity = 83/179 (46.37%), Postives = 95/179 (53.07%), Query Frame = 0

Query: 29  IRYRGVRKRPWGRYAAEIRDPWKKTRVWLGTFDSAEEAARAYDNAARTLRGAKAKTNFPL 88
           +RYRGVRKRPWGRYAAEIRDP+KK+RVWLGTFD+ EEAARAYD  A   RGAKAKTNFP 
Sbjct: 18  VRYRGVRKRPWGRYAAEIRDPFKKSRVWLGTFDTPEEAARAYDKRAIEFRGAKAKTNFPC 77

Query: 89  HPSTISIQQQIPFYQSPPDLHDSNGYGAKIECVQANRPTSSSLSSTVESFSGPRISNPLS 148
                                    Y     C+   +  S S SSTVES S P ++  L 
Sbjct: 78  -------------------------YNINAHCLSLTQ--SLSQSSTVES-SFPNLN--LG 137

Query: 149 SLSMVRRRPVQPVSPD------DCHSDCDSSSSVVDDDEVCVLASFRRALPFDLNLPPP 202
           S S+  R P   +         D  S+ DSSS V+D   V      R  L  DLN PPP
Sbjct: 138 SDSVSSRFPFPKIQVKAGMMVFDERSESDSSSVVMD---VVRYEGRRVVLDLDLNFPPP 163

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022944504.11.1e-121100.00ethylene-responsive transcription factor 3-like [Cucurbita moschata] >XP_0229860... [more]
XP_038902603.11.9e-11896.80ethylene-responsive transcription factor 3-like [Benincasa hispida][more]
XP_004143195.12.5e-11896.80ethylene-responsive transcription factor 3 [Cucumis sativus] >XP_008456400.1 PRE... [more]
XP_023512775.11.1e-11599.05ethylene-responsive transcription factor 3-like isoform X1 [Cucurbita pepo subsp... [more]
XP_023512776.16.2e-11499.52ethylene-responsive transcription factor 3-like isoform X2 [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
Q9SXS83.5e-5155.36Ethylene-responsive transcription factor 3 OS=Nicotiana tabacum OX=4097 GN=ERF3 ... [more]
O803398.6e-5054.51Ethylene-responsive transcription factor 3 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Q9LDE44.4e-4650.60Ethylene-responsive transcription factor 7 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
O803402.7e-2742.33Ethylene-responsive transcription factor 4 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Q9MAI53.0e-2657.14Ethylene-responsive transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=ER... [more]
Match NameE-valueIdentityDescription
A0A6J1J6H05.1e-122100.00ethylene-responsive transcription factor 3-like OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FWT65.1e-122100.00ethylene-responsive transcription factor 3-like OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7UIU51.2e-11896.80Ethylene-responsive transcription factor 3-like OS=Cucumis melo var. makuwa OX=1... [more]
A0A0A0KBH01.2e-11896.80AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G167230 PE=... [more]
A0A1S3C3511.2e-11896.80ethylene-responsive transcription factor 3-like OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT1G50640.16.1e-5154.51ethylene responsive element binding factor 3 [more]
AT3G20310.13.2e-4750.60ethylene response factor 7 [more]
AT3G15210.11.9e-2842.33ethylene responsive element binding factor 4 [more]
AT1G53170.12.1e-2757.14ethylene response factor 8 [more]
AT1G28370.13.1e-2646.37ERF domain protein 11 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 63..83
NoneNo IPR availablePANTHERPTHR31677AP2 DOMAIN CLASS TRANSCRIPTION FACTORcoord: 25..194
NoneNo IPR availablePANTHERPTHR31677:SF157ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 3coord: 25..194
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 31..42
score: 66.67
coord: 53..69
score: 71.55
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 30..93
e-value: 1.6E-35
score: 134.0
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 30..79
e-value: 4.1E-14
score: 52.6
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 30..87
score: 23.419575
IPR001471AP2/ERF domainCDDcd00018AP2coord: 30..87
e-value: 4.13791E-33
score: 111.98
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 29..89
e-value: 1.7E-27
score: 97.3
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 30..88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03460-RACarg03460-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity