Carg03353 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03353
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description50S ribosomal protein L4
LocationCarg_Chr20: 1806900 .. 1809306 (+)
RNA-Seq ExpressionCarg03353
SyntenyCarg03353
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGTACTCCTCTACGACCTGGCGGAGGTGTGATCTTCGGGCCGAAACCTAGAGATTGGTCCGTTAAGATCAACAGAAAGGAGAAGAGATTGGCAATATCGACGGCCGTGGCTAGCGCGGCGGTAAATACGATAGTGGTGGAAGATTTCGGCGATAAGTTCGAGAAGCCGAAAACTAAAGAGTTCATTGCTGCGCTGAAGAGATGGGGAATCGATCCAAAGGAGAAATCACTGTTCTTAATGACGGAGGTTTCTGATAATGTACGATTATCTAGCCGTAACGTCGGAACATTGAAATTGTTGACGCCGAGAACGCTGAATTTGTTCGATATTTTGAATTCCGATAAGTTAGTCTTGACGCCGGCGGCGGTAGATTATCTGAATGAGAGATATGGAAACAATTACGAAGAAGGAATCGCAGACGAGGAAGAAGAAGACGAAGAAGGTAGATTTTCTTACCTTTCTCCAATTCAATTGTTCAATTATAGAAATCAAGAACCTCCATTATCGTTCCATACTTGAGTTTTGCCTAATTCAACATTCAAATCACATTAGAATTATGCTATGAATTCTGGTTCTGCTTCTGCTTCTGTCAACATGGCAAATGCTGGCTTAGGCAATAGTCTGCTTGCTTCCAAATGGGTTCTGCATCATCATTTTGTTGAATCGATTTTCATTCAGATTTCTTGTATTCTCTGTTTTGTTTCATTTCTGTCAGAAATGTTAGAGGAGTAATCAAAATGGAAAGCTTATTCAGGATTGATATTGACTGTCTTCACCCTAAAACCTATGATTTTCTTAACATTGCTCTTTCTTGCTGCTGCTTCCATTGTTTCACACAGAAACTAATGTTTAGAGTGAGCAATTGTAATTCTTCCATTAATTCCTTCTACTGTTTCACATCAAAATGGAGAAGCTCTTGATGAACTCTCCCTTGGAAGTGTGGCTCGAGAGAAATAGTAGAATTTTTAGGGGTAGGAGAAGTCAATGGAGGATGTGTGGGAGGTGGTGAGGGCAACGTATGCTCAAACCTCTAGCCCAAACCCAAGTCCACCACTAACCGATATTGTCCTCTTTGTGTTTTCCCTTTCGGGCTTCCCTTCAAGGTTCCAAAGAAACGTTAAGGAGAGGCTTTCACACCATGTGGGATCTCACCCTTATAAGCAATGTTTCATTCCCCTCCGACCGATGTGGGATCTCACAATCCACCCCCTTATCTAGCGTCTTCGTTGGCACATCACCCGGTATCTATCTCTGATGCCATTTGTAACAGCCAAACCCCACCTCTTTGGGTTTTTTCCTTTCGGTCTTTCCCTCAAGGTTTTTAAACGCGTTTGTTAAGAAAAGGTTTCCACACCCTTATAAAAAGAATGTTTCGTTCCCCTCTCCATCCATGGTGGGATCTCACATCGTGTCTTTCTTTTTTTGGATTGGAATCCGTTCTTGTATCTTGTTGTGAATTTCTTTTATGGGCTTGCTTTATGTAGAAATCTCAGTTTCTTACTACAAAAACAGAACACACCTACTGAAGTTAATAGGGAGAGAGATTGTAGTTCTTCCATTAACATCTTCTTCACTTTTGAAAACAGGGCTGGAGGAGGTAGGTGAGGATTCGGACGCAGCATAATGAATCTGCCATGTGCAAATCATGAAAGGAGCTTTCAGGGTATGTTAACTTTCAAGCTTTGATCGTATTCTTTTTTTGTTCTTATACACACACAACGGTATCTTCTTGTCCTTTTATCCTCCGATATCCTAAAGTGTTATTAGCTGATGATCTCTTCAACAACAATCTTTTATTCTTTTGGAACTTGCATTCTGTTTAGGCTTCCCTCTACTTTCTTCCTAAGTTAGGAGCTTCCATTAGTATTCTGAGTCTCCTATGGAACTTCCTTTGTCCATAACATATGAAAACGTTCCGAGAGAAAGAGAACGAGTTTGCAGAAGATGATACTTAAAATGTTTTGAGTTCCTTGAAAGTCTTCCTAGAAACGGCCCCTTGTTTGGGTGTTTTTAGTTCAATGAGTGAGGTTGAGAGATTTGAACTTTTGACATCTTTGGTCCATAATGTAAGTCTAAATCGTTTGAGCTATGATCAGATTGGCTAAAGTTGAAGACGGGGTCTTGCCCGTAAATTTCAGACATGTAGCTCAAAGATTGTCCGACCCTTTATAACTAGGAAAAATGTGGCTTATAATAGAGTCATGCTTAAACTTTTAGATGATAACCTATGTCTTATTAACCGAGATATGCTTGGTTTGAAAAAACCGTCCCAAGTATTTGACTATTTCTAGAAAGGTTTGTTTTTCTATATCGCATATTTTAAAATTCTCTTAAGATTGATAACAACCAATAATACACACAAATAATTATAATTATTCCGTGATCAAGTAAATCCATGATTTAA

mRNA sequence

ATGCGTACTCCTCTACGACCTGGCGGAGGTGTGATCTTCGGGCCGAAACCTAGAGATTGGTCCGTTAAGATCAACAGAAAGGAGAAGAGATTGGCAATATCGACGGCCGTGGCTAGCGCGGCGGTAAATACGATAGTGGTGGAAGATTTCGGCGATAAGTTCGAGAAGCCGAAAACTAAAGAGTTCATTGCTGCGCTGAAGAGATGGGGAATCGATCCAAAGGAGAAATCACTGTTCTTAATGACGGAGGTTTCTGATAATGTACGATTATCTAGCCGTAACGTCGGAACATTGAAATTGTTGACGCCGAGAACGCTGAATTTGTTCGATATTTTGAATTCCGATAAGTTAGTCTTGACGCCGGCGGCGGTAGATTATCTGAATGAGAGATATGGAAACAATTACGAAGAAGGAATCGCAGACGAGGAAGAAGAAGACGAAGAAGGGCTGGAGGAGGTAGGTGAGGATTCGGACGCAGCATAATGAATCTGCCATGTGCAAATCATGAAAGGAGCTTTCAGGGTATGTTAACTTTCAAGCTTTGATCGTATTCTTTTTTTGTTCTTATACACACACAACGGTATCTTCTTGTCCTTTTATCCTCCGATATCCTAAAGTGTTATTAGCTGATGATCTCTTCAACAACAATCTTTTATTCTTTTGGAACTTGCATTCTGTTTAGGCTTCCCTCTACTTTCTTCCTAAGTTAGGAGCTTCCATTAGTATTCTGAGTCTCCTATGGAACTTCCTTTGTCCATAACATATGAAAACGTTCCGAGAGAAAGAGAACGAGTTTGCAGAAGATGATACTTAAAATGTTTTGAGTTCCTTGAAAGTCTTCCTAGAAACGGCCCCTTGTTTGGGTGTTTTTAGTTCAATGAGTGAGGTTGAGAGATTTGAACTTTTGACATCTTTGGTCCATAATGTAAGTCTAAATCGTTTGAGCTATGATCAGATTGGCTAAAGTTGAAGACGGGGTCTTGCCCGTAAATTTCAGACATGTAGCTCAAAGATTGTCCGACCCTTTATAACTAGGAAAAATGTGGCTTATAATAGAGTCATGCTTAAACTTTTAGATGATAACCTATGTCTTATTAACCGAGATATGCTTGGTTTGAAAAAACCGTCCCAAGTATTTGACTATTTCTAGAAAGGTTTGTTTTTCTATATCGCATATTTTAAAATTCTCTTAAGATTGATAACAACCAATAATACACACAAATAATTATAATTATTCCGTGATCAAGTAAATCCATGATTTAA

Coding sequence (CDS)

ATGCGTACTCCTCTACGACCTGGCGGAGGTGTGATCTTCGGGCCGAAACCTAGAGATTGGTCCGTTAAGATCAACAGAAAGGAGAAGAGATTGGCAATATCGACGGCCGTGGCTAGCGCGGCGGTAAATACGATAGTGGTGGAAGATTTCGGCGATAAGTTCGAGAAGCCGAAAACTAAAGAGTTCATTGCTGCGCTGAAGAGATGGGGAATCGATCCAAAGGAGAAATCACTGTTCTTAATGACGGAGGTTTCTGATAATGTACGATTATCTAGCCGTAACGTCGGAACATTGAAATTGTTGACGCCGAGAACGCTGAATTTGTTCGATATTTTGAATTCCGATAAGTTAGTCTTGACGCCGGCGGCGGTAGATTATCTGAATGAGAGATATGGAAACAATTACGAAGAAGGAATCGCAGACGAGGAAGAAGAAGACGAAGAAGGGCTGGAGGAGGTAGGTGAGGATTCGGACGCAGCATAA

Protein sequence

MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLTPAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA
Homology
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BLAST of Carg03353 vs. NCBI nr
Match: KAG6570669.1 (50S ribosomal protein L4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010514.1 50S ribosomal protein L4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 310.1 bits (793), Expect = 1.1e-80
Identity = 160/160 (100.00%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA 161
           PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA
Sbjct: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA 160

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BLAST of Carg03353 vs. NCBI nr
Match: XP_023512087.1 (50S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023512088.1 50S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 309.7 bits (792), Expect = 1.5e-80
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGVIFGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVIFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA 161
           PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA
Sbjct: 247 PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSDAA 286

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BLAST of Carg03353 vs. NCBI nr
Match: XP_022948154.1 (50S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6605425.1 50S ribosomal protein L4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035374.1 50S ribosomal protein L4, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.7 bits (735), Expect = 6.0e-74
Identity = 150/165 (90.91%), Postives = 155/165 (93.94%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLE-----EVGEDSDAA 161
           PAAVDYLNERYG NYEEGI DEEEE+EE LE     + GED DAA
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEEELEGEEGLDAGEDLDAA 291

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BLAST of Carg03353 vs. NCBI nr
Match: XP_023532270.1 (50S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.3 bits (734), Expect = 7.9e-74
Identity = 150/165 (90.91%), Postives = 155/165 (93.94%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLE-----EVGEDSDAA 161
           PAAVDYLNERYG NYEEGI DEEEE+EE LE     E GE+ DAA
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEEELEGEEGLEAGEELDAA 291

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BLAST of Carg03353 vs. NCBI nr
Match: XP_023007555.1 (50S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 285.8 bits (730), Expect = 2.3e-73
Identity = 151/165 (91.52%), Postives = 155/165 (93.94%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEED-----EEGLEEVGEDSDAA 161
           PAAVDYLNERYG NYEEGI DEEEE+     EEGL E GED DAA
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEELEGEEGL-ETGEDLDAA 290

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BLAST of Carg03353 vs. ExPASy Swiss-Prot
Match: O80361 (50S ribosomal protein L4, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL4 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 5.7e-59
Identity = 115/158 (72.78%), Postives = 140/158 (88.61%), Query Frame = 0 

Query: 2   RTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTKE 61
           RTPLRPGGGV+FGPKP+DWSVKIN+KEKRLAISTA++SA+ NTIVVE+F DKFEKPKTKE
Sbjct: 123 RTPLRPGGGVVFGPKPKDWSVKINKKEKRLAISTALSSASENTIVVEEFNDKFEKPKTKE 182

Query: 62  FIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLTP 121
           FI  ++RWG+DPKEKSLFLMT+VSDNV LSSRN+GTLK+LTPRTLNLFDIL+S+KLVLT 
Sbjct: 183 FIDLMRRWGLDPKEKSLFLMTDVSDNVILSSRNIGTLKMLTPRTLNLFDILDSEKLVLTK 242

Query: 122 AAVDYLNERYG--NNYEEGIADEEEEDEEGLEEVGEDS 158
           +AVD+LNERYG  N +E+   D++E+++ G  E   +S
Sbjct: 243 SAVDFLNERYGDENEWEDEEEDDQEDNDGGEAEESTES 280

[Close]
BLAST of Carg03353 vs. ExPASy Swiss-Prot
Match: O50061 (50S ribosomal protein L4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL4 PE=2 SV=2)

HSP 1 Score: 213.8 bits (543), Expect = 1.5e-54
Identity = 106/154 (68.83%), Postives = 135/154 (87.66%), Query Frame = 0 

Query: 2   RTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAV---NTIVVEDFGDKFEKPK 61
           RTPLRPGGGV+FGP+P+DWS+KINRKEK+LAISTA++SAA      IVVE+FG+KFEKPK
Sbjct: 129 RTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGEKFEKPK 188

Query: 62  TKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLV 121
           TK+F+AA++RWG+DPKEK++FLM +V +NV  SSRN+GTL++LTPRTLNLFDILN+DKLV
Sbjct: 189 TKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDILNADKLV 248

Query: 122 LTPAAVDYLNERYGNNYEEGIADEEEEDEEGLEE 153
           LTPAAV++LN RYG +  E   D++E++ EG EE
Sbjct: 249 LTPAAVEFLNARYGVDAVEE-EDDDEDETEGSEE 281

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BLAST of Carg03353 vs. ExPASy Swiss-Prot
Match: O49937 (50S ribosomal protein L4, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL4 PE=1 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 3.7e-50
Identity = 97/153 (63.40%), Postives = 127/153 (83.01%), Query Frame = 0 

Query: 3   TPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTKEF 62
           +PLRPGGGVIFGPKPRDW++K+N+KE+RLA+STA+ASA  N+ VVE+F + FEKPKTK+F
Sbjct: 131 SPLRPGGGVIFGPKPRDWTIKMNKKERRLALSTAIASAVGNSFVVEEFAENFEKPKTKDF 190

Query: 63  IAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLTPA 122
           IAA++RWG+DP EKSLF + ++ +NV  S RN+ TLKLLTPR+LNLFD+LN++KLV T  
Sbjct: 191 IAAMQRWGLDPAEKSLFFLMDLVENVEKSGRNIRTLKLLTPRSLNLFDVLNAEKLVFTEG 250

Query: 123 AVDYLNERYGNNYEEGIADEEEEDEEGLEEVGE 156
            + YLN+RYG +  E   +EEEE+EEG EEV +
Sbjct: 251 TIQYLNQRYGVDTLEDEDEEEEEEEEG-EEVDD 282

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BLAST of Carg03353 vs. ExPASy Swiss-Prot
Match: B0JI02 (50S ribosomal protein L4 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=rplD PE=3 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-35
Identity = 71/132 (53.79%), Postives = 99/132 (75.00%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           +R+PL  GGGVIFGPKPRD+ VK+NRKEKRLA+ TA+ S A N IVVE F ++F +PKTK
Sbjct: 78  IRSPLWRGGGVIFGPKPRDFEVKVNRKEKRLALRTALISQADNFIVVESFAEQFSQPKTK 137

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           E  AAL RWG  P+EK L ++TE+ +NV LS RN+  LK++   +LN++D++ +D+++ T
Sbjct: 138 ELTAALSRWGASPEEKILLILTEIPENVYLSGRNICNLKIIRADSLNVYDVILADRVIAT 197

Query: 121 PAAVDYLNERYG 133
            AA+  + E YG
Sbjct: 198 AAALAKIEEVYG 209

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BLAST of Carg03353 vs. ExPASy Swiss-Prot
Match: Q8DMN0 (50S ribosomal protein L4 OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=rplD PE=3 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 7.5e-35
Identity = 71/133 (53.38%), Postives = 99/133 (74.44%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           +R+PL  GGGVIFGPKPRD+S K+NRKE+RLA+ TA+ S   + IVV+DF D   +PKTK
Sbjct: 78  IRSPLWRGGGVIFGPKPRDYSQKMNRKERRLALRTALMSRVEDLIVVQDFADHLPRPKTK 137

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           E + AL+RWGI+P++K L L  ++++ V LS+RNV TLKLL    LN+FD+L +D++V T
Sbjct: 138 ELVTALQRWGIEPEQKVLLLAADITETVALSARNVPTLKLLRADQLNVFDLLYADRIVAT 197

Query: 121 PAAVDYLNERYGN 134
             A+  + E YG+
Sbjct: 198 TGAIAKIQEVYGD 210

[Close]
BLAST of Carg03353 vs. ExPASy TrEMBL
Match: A0A6J1G917 (50S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451819 PE=3 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.9e-74
Identity = 150/165 (90.91%), Postives = 155/165 (93.94%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLE-----EVGEDSDAA 161
           PAAVDYLNERYG NYEEGI DEEEE+EE LE     + GED DAA
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEEELEGEEGLDAGEDLDAA 291

[Close]
BLAST of Carg03353 vs. ExPASy TrEMBL
Match: A0A6J1L3A5 (50S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111500015 PE=3 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.1e-73
Identity = 151/165 (91.52%), Postives = 155/165 (93.94%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEED-----EEGLEEVGEDSDAA 161
           PAAVDYLNERYG NYEEGI DEEEE+     EEGL E GED DAA
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEELEGEEGL-ETGEDLDAA 290

[Close]
BLAST of Carg03353 vs. ExPASy TrEMBL
Match: A0A1S3CM35 (50S ribosomal protein L4, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502034 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.2e-73
Identity = 148/163 (90.80%), Postives = 155/163 (95.09%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVE+FGDKFEKPKTK
Sbjct: 128 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEEFGDKFEKPKTK 187

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDIL+SDKLVLT
Sbjct: 188 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILDSDKLVLT 247

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLE---EVGEDSDAA 161
           PAAVDYLNERYG NYEEGI +EEEE+EE  E   E GEDSDAA
Sbjct: 248 PAAVDYLNERYGINYEEGIEEEEEEEEEEEEEGVEAGEDSDAA 290

[Close]
BLAST of Carg03353 vs. ExPASy TrEMBL
Match: A0A6J1G8Y3 (50S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451819 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 7.2e-73
Identity = 146/158 (92.41%), Postives = 152/158 (96.20%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEEGLEEVGEDSD 159
           PAAVDYLNERYG NYEEGI DEEEE+EE LE  GE+ +
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEEELE--GEEEE 282

[Close]
BLAST of Carg03353 vs. ExPASy TrEMBL
Match: A0A6J1KZ16 (50S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111500015 PE=3 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 1.2e-72
Identity = 142/148 (95.95%), Postives = 146/148 (98.65%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 60
           MRTPLRPGGGV+FGPKPRDWS+KINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK
Sbjct: 127 MRTPLRPGGGVVFGPKPRDWSIKINRKEKRLAISTAVASAAVNTIVVEDFGDKFEKPKTK 186

Query: 61  EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLVLT 120
           EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRN+GTLKLLTPRTLNLFDILNSDKLVLT
Sbjct: 187 EFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNIGTLKLLTPRTLNLFDILNSDKLVLT 246

Query: 121 PAAVDYLNERYGNNYEEGIADEEEEDEE 149
           PAAVDYLNERYG NYEEGI DEEEE+EE
Sbjct: 247 PAAVDYLNERYGINYEEGIEDEEEEEEE 274

[Close]
BLAST of Carg03353 vs. TAIR 10
Match: AT1G07320.2 (ribosomal protein L4 )

HSP 1 Score: 215.3 bits (547), Expect = 3.5e-56
Identity = 105/150 (70.00%), Postives = 132/150 (88.00%), Query Frame = 0 

Query: 2   RTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAV---NTIVVEDFGDKFEKPK 61
           RTPLRPGGGV+FGP+P+DWS+KINRKEK+LAISTA++SAA      IVVE+FG+KFEKPK
Sbjct: 129 RTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGEKFEKPK 188

Query: 62  TKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLV 121
           TK+F+AA++RWG+DPKEK++FLM +V +NV  SSRN+GTL++LTPRTLNLFDILN+DKLV
Sbjct: 189 TKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDILNADKLV 248

Query: 122 LTPAAVDYLNERYGNNYEEGIADEEEEDEE 149
           LTPAAV++LN RYG +  E   D+E+E EE
Sbjct: 249 LTPAAVEFLNARYGVDAVEEEDDDEDETEE 278

[Close]
BLAST of Carg03353 vs. TAIR 10
Match: AT1G07320.3 (ribosomal protein L4 )

HSP 1 Score: 214.2 bits (544), Expect = 7.9e-56
Identity = 104/149 (69.80%), Postives = 131/149 (87.92%), Query Frame = 0 

Query: 2   RTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAV---NTIVVEDFGDKFEKPK 61
           RTPLRPGGGV+FGP+P+DWS+KINRKEK+LAISTA++SAA      IVVE+FG+KFEKPK
Sbjct: 129 RTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGEKFEKPK 188

Query: 62  TKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLV 121
           TK+F+AA++RWG+DPKEK++FLM +V +NV  SSRN+GTL++LTPRTLNLFDILN+DKLV
Sbjct: 189 TKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDILNADKLV 248

Query: 122 LTPAAVDYLNERYGNNYEEGIADEEEEDE 148
           LTPAAV++LN RYG +  E   D+E+E E
Sbjct: 249 LTPAAVEFLNARYGVDAVEEEDDDEDETE 277

[Close]
BLAST of Carg03353 vs. TAIR 10
Match: AT1G07320.4 (ribosomal protein L4 )

HSP 1 Score: 214.2 bits (544), Expect = 7.9e-56
Identity = 104/149 (69.80%), Postives = 131/149 (87.92%), Query Frame = 0 

Query: 2   RTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAV---NTIVVEDFGDKFEKPK 61
           RTPLRPGGGV+FGP+P+DWS+KINRKEK+LAISTA++SAA      IVVE+FG+KFEKPK
Sbjct: 129 RTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGEKFEKPK 188

Query: 62  TKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLV 121
           TK+F+AA++RWG+DPKEK++FLM +V +NV  SSRN+GTL++LTPRTLNLFDILN+DKLV
Sbjct: 189 TKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDILNADKLV 248

Query: 122 LTPAAVDYLNERYGNNYEEGIADEEEEDE 148
           LTPAAV++LN RYG +  E   D+E+E E
Sbjct: 249 LTPAAVEFLNARYGVDAVEEEDDDEDETE 277

[Close]
BLAST of Carg03353 vs. TAIR 10
Match: AT1G07320.1 (ribosomal protein L4 )

HSP 1 Score: 213.8 bits (543), Expect = 1.0e-55
Identity = 106/154 (68.83%), Postives = 135/154 (87.66%), Query Frame = 0 

Query: 2   RTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAV---NTIVVEDFGDKFEKPK 61
           RTPLRPGGGV+FGP+P+DWS+KINRKEK+LAISTA++SAA      IVVE+FG+KFEKPK
Sbjct: 129 RTPLRPGGGVVFGPRPKDWSIKINRKEKKLAISTALSSAASAEGGAIVVEEFGEKFEKPK 188

Query: 62  TKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLV 121
           TK+F+AA++RWG+DPKEK++FLM +V +NV  SSRN+GTL++LTPRTLNLFDILN+DKLV
Sbjct: 189 TKDFLAAMQRWGLDPKEKAMFLMIDVDENVAKSSRNIGTLRMLTPRTLNLFDILNADKLV 248

Query: 122 LTPAAVDYLNERYGNNYEEGIADEEEEDEEGLEE 153
           LTPAAV++LN RYG +  E   D++E++ EG EE
Sbjct: 249 LTPAAVEFLNARYGVDAVEE-EDDDEDETEGSEE 281

[Close]
BLAST of Carg03353 vs. TAIR 10
Match: AT2G20060.1 (Ribosomal protein L4/L1 family )

HSP 1 Score: 52.0 bits (123), Expect = 5.2e-07
Identity = 35/132 (26.52%), Postives = 71/132 (53.79%), Query Frame = 0 

Query: 1   MRTPLRPGGGVIFGPKPRDWSVKINRKEKRLAISTAVASAAV--NTIVVEDFGDKFEKPK 60
           +R P   GG V+ GPKPR  ++K+N++ +RL +  A+ + A     +V +D G      K
Sbjct: 165 LRGPQFRGGCVMHGPKPRSHAIKMNKQVRRLGLKIALTARAAEGKLLVFDDLG--LPTHK 224

Query: 61  TKEFIAALKRWGIDPKEKSLFLMTEVSDNVRLSSRNVGTLKLLTPRTLNLFDILNSDKLV 120
           TK  +    +   + K+  +     + + ++L+++N+  + ++    LN++ IL  D L+
Sbjct: 225 TKNIVNYYNQME-NTKKVLVVEGGPIDEKLKLATQNLHYVNIIPSIGLNVYSILLHDTLL 284

Query: 121 LTPAAVDYLNER 131
           ++  AV+ + ER
Sbjct: 285 MSRDAVNKIVER 293

The following BLAST results are available for this feature:
BLAST of Carg03353 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6570669.11.1e-80100.0050S ribosomal protein L4, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_023512087.11.5e-8099.3850S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_02... [more]
XP_022948154.16.0e-7490.9150S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita moschata] >KA... [more]
XP_023532270.17.9e-7490.9150S ribosomal protein L4, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_023007555.12.3e-7391.5250S ribosomal protein L4, chloroplastic-like isoform X1 [Cucurbita maxima][more]
BLAST of Carg03353 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O803615.7e-5972.7850S ribosomal protein L4, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RPL4 PE=... [more]
O500611.5e-5468.8350S ribosomal protein L4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPL4 ... [more]
O499373.7e-5063.4050S ribosomal protein L4, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPL4 PE=... [more]
B0JI021.5e-3553.7950S ribosomal protein L4 OS=Microcystis aeruginosa (strain NIES-843) OX=449447 G... [more]
Q8DMN07.5e-3553.3850S ribosomal protein L4 OS=Thermosynechococcus elongatus (strain BP-1) OX=19722... [more]
BLAST of Carg03353 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G9172.9e-7490.9150S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1L3A51.1e-7391.5250S ribosomal protein L4, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A1S3CM353.2e-7390.8050S ribosomal protein L4, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1G8Y37.2e-7392.4150S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1KZ161.2e-7295.9550S ribosomal protein L4, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3... [more]
BLAST of Carg03353 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G07320.23.5e-5670.00ribosomal protein L4 [more]
AT1G07320.37.9e-5669.80ribosomal protein L4 [more]
AT1G07320.47.9e-5669.80ribosomal protein L4 [more]
AT1G07320.11.0e-5568.83ribosomal protein L4 [more]
AT2G20060.15.2e-0726.52Ribosomal protein L4/L1 family [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023574Ribosomal protein L4 domain superfamilyGENE3D3.40.1370.10coord: 1..134
e-value: 4.8E-35
score: 122.7
IPR023574Ribosomal protein L4 domain superfamilySUPERFAMILY52166Ribosomal protein L4coord: 2..132
IPR002136Ribosomal protein L4/L1ePFAMPF00573Ribosomal_L4coord: 1..130
e-value: 2.4E-31
score: 108.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..160
IPR01300550S ribosomal protein uL4PANTHERPTHR1074650S RIBOSOMAL PROTEIN L4coord: 1..148

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03353-RACarg03353-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005840 ribosome
molecular_function GO:0003735 structural constituent of ribosome