Carg03347 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03347
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncalmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1
LocationCarg_Chr20: 1829534 .. 1834267 (+)
RNA-Seq ExpressionCarg03347
SyntenyCarg03347
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAAATCACGGAGTAAGCTAGGAAAAAAGAACCGGGAAGAGAGACCGCGCATTATTGCCATTGCGGCGGCCGGCGAAGCAAACCAGTCAGCGAAGGGGCAAAGTCAAGCGCTGAAGCCGACATTTCGCCGCCGTAAGCATGGGATATTTTGGTATGTTCAGTATTTTTAGAACTCTAAAAGCAAAATTTAACCCTAACATTACTCCTTCTGTGCTTGACCAAGAGGCCCAGTCTTTCATGACTTCAAATTAAATGCTTCCATCTAATCATCTCTCCATTAAATTCCCAACTGCTTAAATTTTAGGAAGCGGATAATGATGATCAGATTTCCAAATCGAGAATCGCCTGAATTTTGGTTCTACAAATGAAGAACAGAGACGACTCTGACCCTCACTTCCAGGATCAGAGATCGCAGAGCTCCAACTCCCGTGCCGAACGGCGTTCCGGTGCCCTAAGCAAGCCGAATCATGAATCTATACTTTCATACAAGTACGTAAAAGCGGCTGTTTCTAGATTTTTTAAATCCATTTTCATCGGTCGGAAGAAGAATTCTTCGGATCGATCTGTAATCAGCACCAGAGAAGCTAATAATTCTCGCAGAGAAGTCTCCTGTGAGTTTCCTTGATTTTTGGTTCGTTTTGTTTATTCTTCGGCGAGTTAGATGATTGATTTTGCTTTCTTTGATTTTTAGCTTTTTCGACGGGAAGCTACGGGAGGAACTCGTCGGCTTTGAAGTCGTATGATTCGTATGGTTCATACGGTTCCTCGTCTAGCTATCCCAGCGAATTTTTCGCATCTCCGGAATTCTCGATTAAGGATCTTTATAAGGCGACGGACAATTTCTCTGCTGCTAATGTTGTTGGGACTGGATCGTTTGGAACGGTGTACAAAGGGAAGCTCAAAGACGGTTCTCTTGTTGCCGTAAAGCGAGCCAAAAGGGTACGAAGTTCAAGTTTAAACTTATTTTCTGTTGATTCCTCGAAGTTATGGAACAGGTTGAGTTCATCACGAGTTCGAACTTCAGATTAACAATGGAAAAAGTTGCTAAAGCTATGATTCGAGTGATCATATTAATATAAATTTGATCATTAATTTCTTAGGGTTTAGATTTTTGACAGATTTGATACTTTTGGCAGAATGCGAACGAGAGGTGTTTGCTGAAAGCGTTCTGGAATGAGATACAAGCTCTGTCAAGGATCGAACATTTGAATCTGGTACGTTTGTATGGCTATCTGGAGCAAGGAGATGAGAGGATCATTATTGTTGAATACGTTGGCAATGGAAATCTTCGGGAACATCTCGATGGTAAGCAATTTTGGTGAATCATTCTGAATTGTGATATGTTCTTGATGTATGCATTTAACTCAGAAATCTAGTTTATTGGTATCTTTTGTGCAGGCAAGCGAGGAGACGTCCTTGAGATTGGAGAGCGTCTGGACATTGCCATTGATGTAGCTCATGCTATCACCTACCTTCACATGTACAATGGTATGCACATGATTTTCCTAATATGCATTTGATAGTGCTTCTGTGAGATCCCACATCGGTTAGAGAGGGGAATGAAACATTCCTTAGGGTGTGGGAACCTCTTCCTAGTATACGCATTTTAAAAACCTTGAGGGGAAGTGTGAAAGAGAAAGTTCAAAGAGGACAATATCTGCTAGTAGTGGGTTTGAGCTGTTACAAATGGTATTAGAGCCATACATTGAGAGGTGTGCCAACGAGGATGTTGGGCTCCCAAAGGGGGTGGATTATGAGATCTCATATCGGTTGGAGAGGGGAACAAAACATTTCTTAAGAGGGTGTGGAAACCTCTCCCTAGAATGAAACATTCTTTATAGGGTGTGGAAACCTCTCCCTAGAATGAAACATTCTTTAAAAGGGGTGTGGAAACCTCTCCCTAGAATGAAACATTCTTCATAAGGGTGTAGAAACCTCTCCCTAGCAGACGTGTTTTAAAAACTTTGAGAGAAAGTCCGAAAGGGAAAGTTCTAAGAGGACAATATCTGCTAGCGGTCGACTTAGATTGTTACAGCTCCATATCAATGAGTAGGCTACATACAGTTTAAGGAGTGCAATGAAAAAATCTCTGTCATGCAGTACGAACATCAAAGTTTCTATTATTAGGCTAGAGGCTTAAGAATCCCAATCACTTCAAAATACAAACTACCTCATGTTGTTCAGTTGCTAAGTCAAAGGAACAGTGAGGAATCTGAATCTGAATGTTTAAATGTAACTGACAGATGCGCCAATAATCCATCGAGACATAAAGGCTACAAACATCCTAATCACAGAGAAACTCCGGGCAAAAGTGGCAGATTTTGGGTTTGCACGCCTGGTTGCAGAAGATTCTAACGTTACACATATATCAACCCAAGTTAAAGGAACGGCTGGATACTTGGATCCTGAATACCTAAGGACTTATCAGCTCACTGAGAAGAGTGATGTATACTCCTTCGGGGTGTTGCTGGTAGAGCTCATAACAGGAAGACACCCAATTGAAACAAAGAGGGATGTCAAAGAAAGAGTAACTATAAGATGGGTAAGTCTTTTATTAACTGCACCATTACGTAATTCTGATCCTTTATACCTTGCAATTTTATATCAAATTCTCGTCTGCAATACCTTGGGTGCTTCTTTACATTTTCTAGATAGCGACTATCCTTGTTCAGTTTAGTTTCCCTGCATCTAAAAGTGAATATTCAAATGTCATTTTGAAGATTCTCTGCACGTCCTTTTAGTTCACTAGAGTTATATTCTGGCTACAACGTGTGACACCACGATGAATAAAATTGCAAACTTGTAACAATCTAAGTCCACCGCTAAAAGTTATTGTCCGTTTTGGGCTGTTACGAATCACTATCAACCTCAATTTTTAAAATGTGTCTGCTAGGGAGAGGTTTCCACACCCTTTATAAAAAATGTTTTGTTTCCCCTCTACAACCGATGTGAGATCTCACAATCCACCCCCTTTTAGGGGCCTAGTGTCCTCGCTGGCACACCTCCCAGTGTCTGGCTCTGATACTATTTGTAACGACTCAAGCCCACTACTAGCAGATATTATCCGTTTTTGCCCGTTACGTATTGTCGTCATACTCACGGTTCTTAAAACAAGCCCGCTAGGAAGAAGTTTCCACACCTTTATAAAGAACGCTTTTTTCCCTTTCCCAACTGACAGGGATGTCACAAAACTAAAAGTTTTTATGGGTTGAATTGATTCTTTCTGGCCTCTTAAATGTGAAAGTTCTCATCGCATGACAATTTCATTAGGAGTTGCATACACGTTCTTCCCACTCTTAGTTTTGAATACGTGATATTAATGAATGAAGCCAATGTATTTTCAAGTATGGTGTTTTATTTCACTCATCTTCTTCGTCACATGGTATGTTCCTGTTTACTGCAGACCATTATTAGGTACCAAATTACCAATGCATATTAGGACCTCTAGATATCATGCAAGTAAATCCCACGTGTTTAAGTTGTCTACTGTAGTGTGAGGTCTGGTCTTAATTTTCTCTCTAGAAACTAATGTTTAGAGTAGCTCAAAAAGAACTATAGCAGTTTCTGCCTTGCCTAGACAATACGGACGGGAAAAGGTCTCCAATCTGCCTTATTGTGATCGGAGCATAGTTATTTGTTTTCAGTCAAGTTGTCTATCCCGTCCCTTTCTAGGTTGGAGAGGAGAATGAAATAATCTTTATAAGGGTGTGGAAATCTCTCCATAGCAAATATGTTTTAAAACTGTGAGGCTGACAGCGATACGTAACGGGCCGAAGCAAATAATATCTGCCAGCAGTGGGTTTGGGCTGTTACAAATGGTATCAGAGCCAGATACTGGCAGTGTGCCAGCGAGAGTGCTGAGCCCACAAGGGGGGTGGATTGTGAGATTCCTCCTCGGTTAGAGAGAGGAATAAAGGATTGCTTTTAAGGGTGTGGAAATCTCATCCTAGTAGATGCGTTTTAAACCTGTGAGGCTGACAACGATACGTAACGGGACGAAACGGACAATATCTGCTAGCGGTGGGTTTAGATTGTTATAGAAGTCTTTGAAGAGATGTTTTGTTGGGAACTTAGGGTTAGTAAAATCTGTTTGGACTATTCAAATGAGTATTTTTAGAAATATAAGGTTGTGGTATGAGCCTAAAAACAAACAAATCCAATATGATGCAGGCTATGCAGAAGCTGAAAGATGGAGAAGCTGTGATTGCCATGGATCCCAGGCTGAAAAGGACTTCTGCATCTACCGTAACCATGGAGAAGACGCTCAAATTGGCTCGCCGATGCCTCGATCCCTTGAGACCGTCTCGACCGTCCATGAAAACTTGTTGCGAGGAGTTATGGGGAATTCGAAAGGAATATAGAGATAGATTATTATCTTCTTCTTGCTCGGAGTCGATTCGTTCAGCAGAATTTCCAGGTCAAAATGCAAAAAACAACCAATATGTTTCTTTTGATGATAAGGAGGATGAAGATTTGTACAGCAAATTCCATTCTGTATAAGTCACCAACTTATGGCCATTTCCAGATAAGAGTGAAGCTTTTCATACCTCTTTTCCATAGCTTTTAAGTAACAAGTCCTGGAAGTCTCTCTGTTTTTATTGTATATTTTATATTTTCAAAAGATTCCCCAAACAGCATTTATTCAAAATACATTGCTGCACTTTTCTTTACCGTTTGAATTTTCATATTCATTCTGTTTCCTATCCCATGGGAAACAAGTTTCAATAATA

mRNA sequence

ATGAGAAAATCACGGAGTAAGCTAGGAAAAAAGAACCGGGAAGAGAGACCGCGCATTATTGCCATTGCGGCGGCCGGCGAAGCAAACCAGTCAGCGAAGGGGCAAAGTCAAGCGCTGAAGCCGACATTTCGCCGCCGTAAGCATGGGATATTTTGGAAGCGGATAATGATGATCAGATTTCCAAATCGAGAATCGCCTGAATTTTGGTTCTACAAATGAAGAACAGAGACGACTCTGACCCTCACTTCCAGGATCAGAGATCGCAGAGCTCCAACTCCCGTGCCGAACGGCGTTCCGGTGCCCTAAGCAAGCCGAATCATGAATCTATACTTTCATACAAGTACGTAAAAGCGGCTGTTTCTAGATTTTTTAAATCCATTTTCATCGGTCGGAAGAAGAATTCTTCGGATCGATCTGTAATCAGCACCAGAGAAGCTAATAATTCTCGCAGAGAAGTCTCCTCTTTTTCGACGGGAAGCTACGGGAGGAACTCGTCGGCTTTGAAGTCGTATGATTCGTATGGTTCATACGGTTCCTCGTCTAGCTATCCCAGCGAATTTTTCGCATCTCCGGAATTCTCGATTAAGGATCTTTATAAGGCGACGGACAATTTCTCTGCTGCTAATGTTGTTGGGACTGGATCGTTTGGAACGGTGTACAAAGGGAAGCTCAAAGACGGTTCTCTTGTTGCCGTAAAGCGAGCCAAAAGGAATGCGAACGAGAGGTGTTTGCTGAAAGCGTTCTGGAATGAGATACAAGCTCTGTCAAGGATCGAACATTTGAATCTGGTACGTTTGTATGGCTATCTGGAGCAAGGAGATGAGAGGATCATTATTGTTGAATACGTTGGCAATGGAAATCTTCGGGAACATCTCGATGGCAAGCGAGGAGACGTCCTTGAGATTGGAGAGCGTCTGGACATTGCCATTGATGTAGCTCATGCTATCACCTACCTTCACATGTACAATGATGCGCCAATAATCCATCGAGACATAAAGGCTACAAACATCCTAATCACAGAGAAACTCCGGGCAAAAGTGGCAGATTTTGGGTTTGCACGCCTGGTTGCAGAAGATTCTAACGTTACACATATATCAACCCAAGTTAAAGGAACGGCTGGATACTTGGATCCTGAATACCTAAGGACTTATCAGCTCACTGAGAAGAGTGATGTATACTCCTTCGGGGTGTTGCTGGTAGAGCTCATAACAGGAAGACACCCAATTGAAACAAAGAGGGATGTCAAAGAAAGAGTAACTATAAGATGGGCTATGCAGAAGCTGAAAGATGGAGAAGCTGTGATTGCCATGGATCCCAGGCTGAAAAGGACTTCTGCATCTACCGTAACCATGGAGAAGACGCTCAAATTGGCTCGCCGATGCCTCGATCCCTTGAGACCGTCTCGACCGTCCATGAAAACTTGTTGCGAGGAGTTATGGGGAATTCGAAAGGAATATAGAGATAGATTATTATCTTCTTCTTGCTCGGAGTCGATTCGTTCAGCAGAATTTCCAGGTCAAAATGCAAAAAACAACCAATATGTTTCTTTTGATGATAAGGAGGATGAAGATTTGTACAGCAAATTCCATTCTGTATAAGTCACCAACTTATGGCCATTTCCAGATAAGAGTGAAGCTTTTCATACCTCTTTTCCATAGCTTTTAAGTAACAAGTCCTGGAAGTCTCTCTGTTTTTATTGTATATTTTATATTTTCAAAAGATTCCCCAAACAGCATTTATTCAAAATACATTGCTGCACTTTTCTTTACCGTTTGAATTTTCATATTCATTCTGTTTCCTATCCCATGGGAAACAAGTTTCAATAATA

Coding sequence (CDS)

ATGAAGAACAGAGACGACTCTGACCCTCACTTCCAGGATCAGAGATCGCAGAGCTCCAACTCCCGTGCCGAACGGCGTTCCGGTGCCCTAAGCAAGCCGAATCATGAATCTATACTTTCATACAAGTACGTAAAAGCGGCTGTTTCTAGATTTTTTAAATCCATTTTCATCGGTCGGAAGAAGAATTCTTCGGATCGATCTGTAATCAGCACCAGAGAAGCTAATAATTCTCGCAGAGAAGTCTCCTCTTTTTCGACGGGAAGCTACGGGAGGAACTCGTCGGCTTTGAAGTCGTATGATTCGTATGGTTCATACGGTTCCTCGTCTAGCTATCCCAGCGAATTTTTCGCATCTCCGGAATTCTCGATTAAGGATCTTTATAAGGCGACGGACAATTTCTCTGCTGCTAATGTTGTTGGGACTGGATCGTTTGGAACGGTGTACAAAGGGAAGCTCAAAGACGGTTCTCTTGTTGCCGTAAAGCGAGCCAAAAGGAATGCGAACGAGAGGTGTTTGCTGAAAGCGTTCTGGAATGAGATACAAGCTCTGTCAAGGATCGAACATTTGAATCTGGTACGTTTGTATGGCTATCTGGAGCAAGGAGATGAGAGGATCATTATTGTTGAATACGTTGGCAATGGAAATCTTCGGGAACATCTCGATGGCAAGCGAGGAGACGTCCTTGAGATTGGAGAGCGTCTGGACATTGCCATTGATGTAGCTCATGCTATCACCTACCTTCACATGTACAATGATGCGCCAATAATCCATCGAGACATAAAGGCTACAAACATCCTAATCACAGAGAAACTCCGGGCAAAAGTGGCAGATTTTGGGTTTGCACGCCTGGTTGCAGAAGATTCTAACGTTACACATATATCAACCCAAGTTAAAGGAACGGCTGGATACTTGGATCCTGAATACCTAAGGACTTATCAGCTCACTGAGAAGAGTGATGTATACTCCTTCGGGGTGTTGCTGGTAGAGCTCATAACAGGAAGACACCCAATTGAAACAAAGAGGGATGTCAAAGAAAGAGTAACTATAAGATGGGCTATGCAGAAGCTGAAAGATGGAGAAGCTGTGATTGCCATGGATCCCAGGCTGAAAAGGACTTCTGCATCTACCGTAACCATGGAGAAGACGCTCAAATTGGCTCGCCGATGCCTCGATCCCTTGAGACCGTCTCGACCGTCCATGAAAACTTGTTGCGAGGAGTTATGGGGAATTCGAAAGGAATATAGAGATAGATTATTATCTTCTTCTTGCTCGGAGTCGATTCGTTCAGCAGAATTTCCAGGTCAAAATGCAAAAAACAACCAATATGTTTCTTTTGATGATAAGGAGGATGAAGATTTGTACAGCAAATTCCATTCTGTATAA

Protein sequence

MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRKKNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV
Homology
BLAST of Carg03347 vs. NCBI nr
Match: XP_022944525.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita moschata] >KAG7010520.1 Calmodulin-binding receptor-like cytoplasmic kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 887.1 bits (2291), Expect = 6.5e-254
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60
           MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK
Sbjct: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60

Query: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120
           KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE
Sbjct: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI
Sbjct: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240
           QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV
Sbjct: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240

Query: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300
           AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT
Sbjct: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300

Query: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360
           AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE
Sbjct: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360

Query: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420
           AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS
Sbjct: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420

Query: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 461
           SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV
Sbjct: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 460

BLAST of Carg03347 vs. NCBI nr
Match: XP_022986864.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita maxima] >XP_022986865.1 calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 881.7 bits (2277), Expect = 2.7e-252
Identity = 456/460 (99.13%), Postives = 458/460 (99.57%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60
           MKNRDDSDPHFQDQRSQSSNSRAERRSGAL KPNHES+LSYKYVKAAVSRFFKSIFIGRK
Sbjct: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALRKPNHESVLSYKYVKAAVSRFFKSIFIGRK 60

Query: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120
           KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE
Sbjct: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI
Sbjct: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240
           QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGD LEIGERLDIAIDV
Sbjct: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDGLEIGERLDIAIDV 240

Query: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300
           AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT
Sbjct: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300

Query: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360
           AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRD+KERVTIRWAMQKLKDGE
Sbjct: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDIKERVTIRWAMQKLKDGE 360

Query: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420
           AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS
Sbjct: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420

Query: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 461
           SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV
Sbjct: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 460

BLAST of Carg03347 vs. NCBI nr
Match: XP_023513337.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 879.8 bits (2272), Expect = 1.0e-251
Identity = 456/460 (99.13%), Postives = 458/460 (99.57%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60
           MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK
Sbjct: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60

Query: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120
           KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFF+SPE
Sbjct: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFSSPE 120

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI
Sbjct: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240
           QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV
Sbjct: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240

Query: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300
           AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT
Sbjct: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300

Query: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360
           AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE
Sbjct: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360

Query: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420
           AVIAMDPRL+RTSASTVTMEK LKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS
Sbjct: 361 AVIAMDPRLRRTSASTVTMEKMLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420

Query: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 461
           SSCSESIRSAEFPG NAKNNQYVSFDDKEDEDLYSKFHSV
Sbjct: 421 SSCSESIRSAEFPGGNAKNNQYVSFDDKEDEDLYSKFHSV 460

BLAST of Carg03347 vs. NCBI nr
Match: XP_022944526.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 740.0 bits (1909), Expect = 1.3e-209
Identity = 377/379 (99.47%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 82  SSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 141
           ++FSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT
Sbjct: 7   TTFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 66

Query: 142 GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 201
           GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG
Sbjct: 67  GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 126

Query: 202 DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 261
           DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK
Sbjct: 127 DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 186

Query: 262 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 321
           ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY
Sbjct: 187 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 246

Query: 322 SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 381
           SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK
Sbjct: 247 SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 306

Query: 382 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 441
           TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ
Sbjct: 307 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 366

Query: 442 YVSFDDKEDEDLYSKFHSV 461
           YVSFDDKEDEDLYSKFHSV
Sbjct: 367 YVSFDDKEDEDLYSKFHSV 385

BLAST of Carg03347 vs. NCBI nr
Match: XP_022986866.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 736.9 bits (1901), Expect = 1.1e-208
Identity = 375/379 (98.94%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 82  SSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 141
           ++FSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT
Sbjct: 7   TTFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 66

Query: 142 GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 201
           GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG
Sbjct: 67  GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 126

Query: 202 DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 261
           DERIIIVEYVGNGNLREHLDGKRGD LEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK
Sbjct: 127 DERIIIVEYVGNGNLREHLDGKRGDGLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 186

Query: 262 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 321
           ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY
Sbjct: 187 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 246

Query: 322 SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 381
           SFGVLLVELITGRHPIETKRD+KERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK
Sbjct: 247 SFGVLLVELITGRHPIETKRDIKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 306

Query: 382 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 441
           TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ
Sbjct: 307 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 366

Query: 442 YVSFDDKEDEDLYSKFHSV 461
           YVSFDDKEDEDLYSKFHSV
Sbjct: 367 YVSFDDKEDEDLYSKFHSV 385

BLAST of Carg03347 vs. ExPASy Swiss-Prot
Match: Q9FIL7 (Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CRCK1 PE=1 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 4.5e-109
Identity = 228/467 (48.82%), Postives = 311/467 (66.60%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRS-GALSKPNHESILSYKYVKAAVSRFFKSIFIGR 60
           M+++  +   F + + Q  +S       G   K  + S+L    ++ A  + F  IF+G+
Sbjct: 3   MRSKTPTPLRFSNGKHQRDDSEYSWTDVGTGEKARNVSVLG--AIRRAAKKVFVIIFLGQ 62

Query: 61  KKNS-----SDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSE 120
           +K       SD    ST +  ++    + +S+ S    S+  K    Y   GS    P +
Sbjct: 63  RKLKPTECRSDPGESSTHDRESTLSGWTGYSSPSSFGRSTERKVSGQYRFSGSRFQSPGK 122

Query: 121 FFASPE--------FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRN 180
             +S +        FS  +L +AT NFS+ + +G G FGTV+KGKL DG++VA+KRA++N
Sbjct: 123 DSSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGFGTVFKGKLDDGTIVAIKRARKN 182

Query: 181 ANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGD 240
              +  L  F NEI  LS+IEH+NLV+LYG+LE GDE++I+VEYV NGNLREHLDG RG+
Sbjct: 183 NYGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANGNLREHLDGLRGN 242

Query: 241 VLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAE 300
            LE+ ERL+IAIDVAHA+TYLH Y D+PIIHRDIKA+NILIT KLRAKVADFGFARLV+E
Sbjct: 243 RLEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAKVADFGFARLVSE 302

Query: 301 DSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKER 360
           D   THISTQVKG+AGY+DP+YLRT+QLT+KSDVYSFGVLLVE++TGR PIE KR  K+R
Sbjct: 303 DLGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGRRPIELKRPRKDR 362

Query: 361 VTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEE 420
           +T++WA+++LKD EAV+ MDP LKR  A+    EK L+LA  C+ P R +RP+MK   E+
Sbjct: 363 LTVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTRATRPAMKGIAEK 422

Query: 421 LWGIRKEYRDRLLSSSCSESIRSA---EFPGQNAKNNQYVSFDDKED 451
           LW IR+E ++ ++ SS S S  S+    F G+++        +D E+
Sbjct: 423 LWAIRREMKETMICSSASNSSCSSTTHSFIGRDSDRYALPRIEDNEN 467

BLAST of Carg03347 vs. ExPASy Swiss-Prot
Match: Q8VZJ9 (Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CRCK2 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.5e-99
Identity = 199/371 (53.64%), Postives = 255/371 (68.73%), Query Frame = 0

Query: 54  SIFIGRKKNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPS 113
           S +I   K+SSD ++ S R            STGS    SS  +SY +      +     
Sbjct: 66  SNYIDNSKSSSDNNIRSRRS-----------STGS----SSVQRSYGNANETEHT----- 125

Query: 114 EFFASPEFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANE--RC 173
                  F+  ++Y AT NFS +  +G G FGTVYK KL+DG   AVKRAK++ ++  + 
Sbjct: 126 ------RFTFDEIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQG 185

Query: 174 LLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIG 233
               F +EIQ L+++ HL+LV+ YG++   DE+I++VEYV NG LR+HLD K G  L++ 
Sbjct: 186 ADAEFMSEIQTLAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMA 245

Query: 234 ERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAE-DSNV 293
            RLDIA DVAHAITYLHMY   PIIHRDIK++NIL+TE  RAKVADFGFARL  + DS  
Sbjct: 246 TRLDIATDVAHAITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGA 305

Query: 294 THISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIR 353
           TH+STQVKGTAGYLDPEYL TYQLTEKSDVYSFGVLLVEL+TGR PIE  R  KER+TIR
Sbjct: 306 THVSTQVKGTAGYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIR 365

Query: 354 WAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGI 413
           WA++K   G+ +  +DP+L++ SA+ + +EK L++A +CL P R SRPSMK C E LWGI
Sbjct: 366 WAIKKFTSGDTISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGI 410

Query: 414 RKEYRDRLLSS 422
           RK+YR+ L +S
Sbjct: 426 RKDYRELLNTS 410

BLAST of Carg03347 vs. ExPASy Swiss-Prot
Match: Q9ASQ5 (Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRCK3 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.4e-80
Identity = 175/369 (47.43%), Postives = 228/369 (61.79%), Query Frame = 0

Query: 59  RKKNSSDRSVISTREANNSRREVSSF--STGSYGRNSSALKSYDSYGSYGSSSSYPSEFF 118
           RK NS +   +  +E +NS  +VSSF  S  S     S  ++  S      S   PS + 
Sbjct: 148 RKANSHE---VLPKE-SNSVHQVSSFEMSPSSEKIPQSPFRAPPSPSRVPQS---PSRYA 207

Query: 119 ASP--------EFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNAN 178
            SP          ++  +  AT NF+ ++ +G G FG V+KG L DG +VA+KRAK+   
Sbjct: 208 MSPRPSRLGPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVLDDGQVVAIKRAKKEHF 267

Query: 179 ERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVL 238
           E  L   F +E+  LS+I H NLV+L GY+++GDER+II EYV NG LR+HLDG RG  L
Sbjct: 268 EN-LRTEFKSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRNGTLRDHLDGARGTKL 327

Query: 239 EIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDS 298
              +RL+I IDV H +TYLH Y +  IIHRDIK++NIL+T+ +RAKVADFGFAR    DS
Sbjct: 328 NFNQRLEIVIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRAKVADFGFARGGPTDS 387

Query: 299 NVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVT 358
           N THI TQVKGT GYLDPEY++TY LT KSDVYSFG+LLVE++TGR P+E KR   ER+T
Sbjct: 388 NQTHILTQVKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTGRRPVEAKRLPDERIT 447

Query: 359 IRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELW 418
           +RWA  K  +G     +DP   R       + K   LA +C  P +  RP M+   ++LW
Sbjct: 448 VRWAFDKYNEGRVFELVDPN-ARERVDEKILRKMFSLAFQCAAPTKKERPDMEAVGKQLW 507

BLAST of Carg03347 vs. ExPASy Swiss-Prot
Match: Q8GZ99 (Leucine-rich repeat receptor protein kinase HPCA1 OS=Arabidopsis thaliana OX=3702 GN=HPCA1 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.0e-61
Identity = 136/307 (44.30%), Postives = 192/307 (62.54%), Query Frame = 0

Query: 108 SSSYPSEFFASPEFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNA 167
           SS    +   +  F+ ++L K TDNFS AN VG G +G VY+G L +G L+A+KRA++ +
Sbjct: 606 SSIDAPQLMGAKAFTFEELKKCTDNFSEANDVGGGGYGKVYRGILPNGQLIAIKRAQQGS 665

Query: 168 NERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDV 227
            +  L   F  EI+ LSR+ H N+VRL G+    +E++++ EY+ NG+L++ L GK G  
Sbjct: 666 LQGGL--EFKTEIELLSRVHHKNVVRLLGFCFDRNEQMLVYEYISNGSLKDSLSGKSGIR 725

Query: 228 LEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAED 287
           L+   RL IA+     + YLH   D PIIHRDIK+ NIL+ E L AKVADFG ++LV  D
Sbjct: 726 LDWTRRLKIALGSGKGLAYLHELADPPIIHRDIKSNNILLDENLTAKVADFGLSKLVG-D 785

Query: 288 SNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIE----TKRDV 347
              TH++TQVKGT GYLDPEY  T QLTEKSDVY FGV+L+EL+TGR PIE      R+V
Sbjct: 786 PEKTHVTTQVKGTMGYLDPEYYMTNQLTEKSDVYGFGVVLLELLTGRSPIERGKYVVREV 845

Query: 348 KERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTC 407
           K ++    ++  L++      +D  +  +S +    EK + LA RC++    +RPSM   
Sbjct: 846 KTKMNKSRSLYDLQE-----LLDTTIIASSGNLKGFEKYVDLALRCVEEEGVNRPSMGEV 904

Query: 408 CEELWGI 411
            +E+  I
Sbjct: 906 VKEIENI 904

BLAST of Carg03347 vs. ExPASy Swiss-Prot
Match: Q9S9M5 (Wall-associated receptor kinase-like 1 OS=Arabidopsis thaliana OX=3702 GN=WAKL1 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 4.3e-59
Identity = 138/297 (46.46%), Postives = 182/297 (61.28%), Query Frame = 0

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FS K+L KATDNFS   V+G G  GTVYKG L DGS+VAVKR+K    ++  ++ F NEI
Sbjct: 417 FSSKELRKATDNFSIDRVLGQGGQGTVYKGMLVDGSIVAVKRSKVVDEDK--MEEFINEI 476

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGE-RLDIAID 240
             LS+I H N+V+L G   + +  I++ EY+ NG+L + L  +  D     E RL IAI+
Sbjct: 477 VLLSQINHRNIVKLLGCCLETEVPILVYEYIPNGDLFKRLHDESDDYTMTWEVRLRIAIE 536

Query: 241 VAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKG 300
           +A A+TY+H     PI HRDIK TNIL+ EK RAKV+DFG +R V  D   TH++T V G
Sbjct: 537 IAGALTYMHSAASFPIFHRDIKTTNILLDEKYRAKVSDFGTSRSVTLDQ--THLTTLVAG 596

Query: 301 TAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDG 360
           T GY+DPEY  + Q T KSDVYSFGV+LVELITG  P+   R  + R      ++ +K+ 
Sbjct: 597 TFGYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPLSRVRSEEGRGLATHFLEAMKEN 656

Query: 361 EAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRD 417
             +  +D R+K  S     M    KLAR+CL+    +RP+MK    EL  IR    D
Sbjct: 657 RVIDIIDIRIKDESKLEQVM-AVAKLARKCLNRKGKNRPNMKEVSNELERIRSSPED 708

BLAST of Carg03347 vs. ExPASy TrEMBL
Match: A0A6J1FYQ3 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448958 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.1e-254
Identity = 460/460 (100.00%), Postives = 460/460 (100.00%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60
           MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK
Sbjct: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60

Query: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120
           KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE
Sbjct: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI
Sbjct: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240
           QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV
Sbjct: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240

Query: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300
           AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT
Sbjct: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300

Query: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360
           AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE
Sbjct: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360

Query: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420
           AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS
Sbjct: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420

Query: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 461
           SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV
Sbjct: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 460

BLAST of Carg03347 vs. ExPASy TrEMBL
Match: A0A6J1JHA2 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484476 PE=3 SV=1)

HSP 1 Score: 881.7 bits (2277), Expect = 1.3e-252
Identity = 456/460 (99.13%), Postives = 458/460 (99.57%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAAVSRFFKSIFIGRK 60
           MKNRDDSDPHFQDQRSQSSNSRAERRSGAL KPNHES+LSYKYVKAAVSRFFKSIFIGRK
Sbjct: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALRKPNHESVLSYKYVKAAVSRFFKSIFIGRK 60

Query: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120
           KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE
Sbjct: 61  KNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPE 120

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI
Sbjct: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDV 240
           QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGD LEIGERLDIAIDV
Sbjct: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDGLEIGERLDIAIDV 240

Query: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300
           AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT
Sbjct: 241 AHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGT 300

Query: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGE 360
           AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRD+KERVTIRWAMQKLKDGE
Sbjct: 301 AGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDIKERVTIRWAMQKLKDGE 360

Query: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420
           AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS
Sbjct: 361 AVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLS 420

Query: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 461
           SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV
Sbjct: 421 SSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHSV 460

BLAST of Carg03347 vs. ExPASy TrEMBL
Match: A0A6J1FUN1 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448958 PE=3 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 6.2e-210
Identity = 377/379 (99.47%), Postives = 379/379 (100.00%), Query Frame = 0

Query: 82  SSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 141
           ++FSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT
Sbjct: 7   TTFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 66

Query: 142 GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 201
           GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG
Sbjct: 67  GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 126

Query: 202 DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 261
           DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK
Sbjct: 127 DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 186

Query: 262 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 321
           ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY
Sbjct: 187 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 246

Query: 322 SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 381
           SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK
Sbjct: 247 SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 306

Query: 382 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 441
           TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ
Sbjct: 307 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 366

Query: 442 YVSFDDKEDEDLYSKFHSV 461
           YVSFDDKEDEDLYSKFHSV
Sbjct: 367 YVSFDDKEDEDLYSKFHSV 385

BLAST of Carg03347 vs. ExPASy TrEMBL
Match: A0A6J1JCG5 (calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484476 PE=3 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 5.2e-209
Identity = 375/379 (98.94%), Postives = 378/379 (99.74%), Query Frame = 0

Query: 82  SSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 141
           ++FSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT
Sbjct: 7   TTFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFASPEFSIKDLYKATDNFSAANVVGT 66

Query: 142 GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 201
           GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG
Sbjct: 67  GSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQG 126

Query: 202 DERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 261
           DERIIIVEYVGNGNLREHLDGKRGD LEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK
Sbjct: 127 DERIIIVEYVGNGNLREHLDGKRGDGLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIK 186

Query: 262 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 321
           ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY
Sbjct: 187 ATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVY 246

Query: 322 SFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 381
           SFGVLLVELITGRHPIETKRD+KERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK
Sbjct: 247 SFGVLLVELITGRHPIETKRDIKERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEK 306

Query: 382 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 441
           TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ
Sbjct: 307 TLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDRLLSSSCSESIRSAEFPGQNAKNNQ 366

Query: 442 YVSFDDKEDEDLYSKFHSV 461
           YVSFDDKEDEDLYSKFHSV
Sbjct: 367 YVSFDDKEDEDLYSKFHSV 385

BLAST of Carg03347 vs. ExPASy TrEMBL
Match: A0A5D3CVK9 (Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00430 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 5.2e-201
Identity = 378/462 (81.82%), Postives = 413/462 (89.39%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRSGALSKPNHESILSYKYVKAA---VSRFFKSIFI 60
           MKN +DS   F  QRSQSS SR ERRSGAL+K N++S+LSYK+VKAA   VSRFFKSIF+
Sbjct: 1   MKNTNDSTSQFHSQRSQSSKSRNERRSGALNKRNNDSVLSYKFVKAAVNRVSRFFKSIFV 60

Query: 61  GRKKNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSEFFA 120
           GRKK+SSD SV   +EAN+ R    +FSTGS+GRNS ALKS  SYGSYGSSSS P+EFFA
Sbjct: 61  GRKKDSSDPSVFGNKEANSPR----AFSTGSFGRNSLALKSSGSYGSYGSSSSLPNEFFA 120

Query: 121 SPEFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFW 180
           +  F+I+++Y+AT NFSA NV+G G+FGTVYKGKLKDGSLVAVKRAKRNA+ER LL  F 
Sbjct: 121 AAGFTIEEVYRATGNFSAGNVLGAGAFGTVYKGKLKDGSLVAVKRAKRNASERRLLTEFR 180

Query: 181 NEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGERLDIA 240
           NEIQ LSRIEHLNLVRLYG+LEQGDERI+IVEYVGNGNLREHLDGKRG  LEIGERLDIA
Sbjct: 181 NEIQTLSRIEHLNLVRLYGFLEQGDERIMIVEYVGNGNLREHLDGKRGVGLEIGERLDIA 240

Query: 241 IDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQV 300
           IDVAHAITYLHMYNDAPIIHRDIKATNILIT+KLRAKVADFGFARLV EDSNVTH+STQV
Sbjct: 241 IDVAHAITYLHMYNDAPIIHRDIKATNILITDKLRAKVADFGFARLVLEDSNVTHVSTQV 300

Query: 301 KGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLK 360
           KGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVEL+TGRHPIETKRD+KERVTIRWAMQKLK
Sbjct: 301 KGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELMTGRHPIETKRDIKERVTIRWAMQKLK 360

Query: 361 DGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRDR 420
           +GEAVIAMDPRL+RTSASTVTME  LKLARRCLDP RPSRPSMKTC EELWGIRKEY+DR
Sbjct: 361 EGEAVIAMDPRLRRTSASTVTMENMLKLARRCLDPSRPSRPSMKTCGEELWGIRKEYKDR 420

Query: 421 LLSSSCSESIRSAEFPGQNAKNNQYVSFDDKEDEDLYSKFHS 460
           LLSSS SES+RSA+FP QNA+NN Y SF  KEDEDLY+KF S
Sbjct: 421 LLSSSYSESLRSADFPAQNARNNLYESFGIKEDEDLYNKFIS 458

BLAST of Carg03347 vs. TAIR 10
Match: AT5G58940.1 (calmodulin-binding receptor-like cytoplasmic kinase 1 )

HSP 1 Score: 396.4 bits (1017), Expect = 3.2e-110
Identity = 228/467 (48.82%), Postives = 311/467 (66.60%), Query Frame = 0

Query: 1   MKNRDDSDPHFQDQRSQSSNSRAERRS-GALSKPNHESILSYKYVKAAVSRFFKSIFIGR 60
           M+++  +   F + + Q  +S       G   K  + S+L    ++ A  + F  IF+G+
Sbjct: 3   MRSKTPTPLRFSNGKHQRDDSEYSWTDVGTGEKARNVSVLG--AIRRAAKKVFVIIFLGQ 62

Query: 61  KKNS-----SDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPSE 120
           +K       SD    ST +  ++    + +S+ S    S+  K    Y   GS    P +
Sbjct: 63  RKLKPTECRSDPGESSTHDRESTLSGWTGYSSPSSFGRSTERKVSGQYRFSGSRFQSPGK 122

Query: 121 FFASPE--------FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRN 180
             +S +        FS  +L +AT NFS+ + +G G FGTV+KGKL DG++VA+KRA++N
Sbjct: 123 DSSSSKSWHQGPVIFSFGELQRATANFSSVHQIGEGGFGTVFKGKLDDGTIVAIKRARKN 182

Query: 181 ANERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGD 240
              +  L  F NEI  LS+IEH+NLV+LYG+LE GDE++I+VEYV NGNLREHLDG RG+
Sbjct: 183 NYGKSWLLEFKNEIYTLSKIEHMNLVKLYGFLEHGDEKVIVVEYVANGNLREHLDGLRGN 242

Query: 241 VLEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAE 300
            LE+ ERL+IAIDVAHA+TYLH Y D+PIIHRDIKA+NILIT KLRAKVADFGFARLV+E
Sbjct: 243 RLEMAERLEIAIDVAHALTYLHTYTDSPIIHRDIKASNILITNKLRAKVADFGFARLVSE 302

Query: 301 DSNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKER 360
           D   THISTQVKG+AGY+DP+YLRT+QLT+KSDVYSFGVLLVE++TGR PIE KR  K+R
Sbjct: 303 DLGATHISTQVKGSAGYVDPDYLRTFQLTDKSDVYSFGVLLVEILTGRRPIELKRPRKDR 362

Query: 361 VTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEE 420
           +T++WA+++LKD EAV+ MDP LKR  A+    EK L+LA  C+ P R +RP+MK   E+
Sbjct: 363 LTVKWALRRLKDDEAVLIMDPFLKRNRAAIEVAEKMLRLASECVTPTRATRPAMKGIAEK 422

Query: 421 LWGIRKEYRDRLLSSSCSESIRSA---EFPGQNAKNNQYVSFDDKED 451
           LW IR+E ++ ++ SS S S  S+    F G+++        +D E+
Sbjct: 423 LWAIRREMKETMICSSASNSSCSSTTHSFIGRDSDRYALPRIEDNEN 467

BLAST of Carg03347 vs. TAIR 10
Match: AT4G00330.1 (calmodulin-binding receptor-like cytoplasmic kinase 2 )

HSP 1 Score: 364.0 bits (933), Expect = 1.8e-100
Identity = 199/371 (53.64%), Postives = 255/371 (68.73%), Query Frame = 0

Query: 54  SIFIGRKKNSSDRSVISTREANNSRREVSSFSTGSYGRNSSALKSYDSYGSYGSSSSYPS 113
           S +I   K+SSD ++ S R            STGS    SS  +SY +      +     
Sbjct: 66  SNYIDNSKSSSDNNIRSRRS-----------STGS----SSVQRSYGNANETEHT----- 125

Query: 114 EFFASPEFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANE--RC 173
                  F+  ++Y AT NFS +  +G G FGTVYK KL+DG   AVKRAK++ ++  + 
Sbjct: 126 ------RFTFDEIYDATKNFSPSFRIGQGGFGTVYKVKLRDGKTFAVKRAKKSMHDDRQG 185

Query: 174 LLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIG 233
               F +EIQ L+++ HL+LV+ YG++   DE+I++VEYV NG LR+HLD K G  L++ 
Sbjct: 186 ADAEFMSEIQTLAQVTHLSLVKYYGFVVHNDEKILVVEYVANGTLRDHLDCKEGKTLDMA 245

Query: 234 ERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAE-DSNV 293
            RLDIA DVAHAITYLHMY   PIIHRDIK++NIL+TE  RAKVADFGFARL  + DS  
Sbjct: 246 TRLDIATDVAHAITYLHMYTQPPIIHRDIKSSNILLTENYRAKVADFGFARLAPDTDSGA 305

Query: 294 THISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIR 353
           TH+STQVKGTAGYLDPEYL TYQLTEKSDVYSFGVLLVEL+TGR PIE  R  KER+TIR
Sbjct: 306 THVSTQVKGTAGYLDPEYLTTYQLTEKSDVYSFGVLLVELLTGRRPIELSRGQKERITIR 365

Query: 354 WAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGI 413
           WA++K   G+ +  +DP+L++ SA+ + +EK L++A +CL P R SRPSMK C E LWGI
Sbjct: 366 WAIKKFTSGDTISVLDPKLEQNSANNLALEKVLEMAFQCLAPHRRSRPSMKKCSEILWGI 410

Query: 414 RKEYRDRLLSS 422
           RK+YR+ L +S
Sbjct: 426 RKDYRELLNTS 410

BLAST of Carg03347 vs. TAIR 10
Match: AT2G11520.1 (calmodulin-binding receptor-like cytoplasmic kinase 3 )

HSP 1 Score: 300.1 bits (767), Expect = 3.1e-81
Identity = 175/369 (47.43%), Postives = 228/369 (61.79%), Query Frame = 0

Query: 59  RKKNSSDRSVISTREANNSRREVSSF--STGSYGRNSSALKSYDSYGSYGSSSSYPSEFF 118
           RK NS +   +  +E +NS  +VSSF  S  S     S  ++  S      S   PS + 
Sbjct: 148 RKANSHE---VLPKE-SNSVHQVSSFEMSPSSEKIPQSPFRAPPSPSRVPQS---PSRYA 207

Query: 119 ASP--------EFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNAN 178
            SP          ++  +  AT NF+ ++ +G G FG V+KG L DG +VA+KRAK+   
Sbjct: 208 MSPRPSRLGPLNLTMSQINTATGNFADSHQIGEGGFGVVFKGVLDDGQVVAIKRAKKEHF 267

Query: 179 ERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVL 238
           E  L   F +E+  LS+I H NLV+L GY+++GDER+II EYV NG LR+HLDG RG  L
Sbjct: 268 EN-LRTEFKSEVDLLSKIGHRNLVKLLGYVDKGDERLIITEYVRNGTLRDHLDGARGTKL 327

Query: 239 EIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDS 298
              +RL+I IDV H +TYLH Y +  IIHRDIK++NIL+T+ +RAKVADFGFAR    DS
Sbjct: 328 NFNQRLEIVIDVCHGLTYLHSYAERQIIHRDIKSSNILLTDSMRAKVADFGFARGGPTDS 387

Query: 299 NVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVT 358
           N THI TQVKGT GYLDPEY++TY LT KSDVYSFG+LLVE++TGR P+E KR   ER+T
Sbjct: 388 NQTHILTQVKGTVGYLDPEYMKTYHLTAKSDVYSFGILLVEILTGRRPVEAKRLPDERIT 447

Query: 359 IRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELW 418
           +RWA  K  +G     +DP   R       + K   LA +C  P +  RP M+   ++LW
Sbjct: 448 VRWAFDKYNEGRVFELVDPN-ARERVDEKILRKMFSLAFQCAAPTKKERPDMEAVGKQLW 507

BLAST of Carg03347 vs. TAIR 10
Match: AT5G49760.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 236.5 bits (602), Expect = 4.3e-62
Identity = 136/307 (44.30%), Postives = 192/307 (62.54%), Query Frame = 0

Query: 108 SSSYPSEFFASPEFSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNA 167
           SS    +   +  F+ ++L K TDNFS AN VG G +G VY+G L +G L+A+KRA++ +
Sbjct: 606 SSIDAPQLMGAKAFTFEELKKCTDNFSEANDVGGGGYGKVYRGILPNGQLIAIKRAQQGS 665

Query: 168 NERCLLKAFWNEIQALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDV 227
            +  L   F  EI+ LSR+ H N+VRL G+    +E++++ EY+ NG+L++ L GK G  
Sbjct: 666 LQGGL--EFKTEIELLSRVHHKNVVRLLGFCFDRNEQMLVYEYISNGSLKDSLSGKSGIR 725

Query: 228 LEIGERLDIAIDVAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAED 287
           L+   RL IA+     + YLH   D PIIHRDIK+ NIL+ E L AKVADFG ++LV  D
Sbjct: 726 LDWTRRLKIALGSGKGLAYLHELADPPIIHRDIKSNNILLDENLTAKVADFGLSKLVG-D 785

Query: 288 SNVTHISTQVKGTAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIE----TKRDV 347
              TH++TQVKGT GYLDPEY  T QLTEKSDVY FGV+L+EL+TGR PIE      R+V
Sbjct: 786 PEKTHVTTQVKGTMGYLDPEYYMTNQLTEKSDVYGFGVVLLELLTGRSPIERGKYVVREV 845

Query: 348 KERVTIRWAMQKLKDGEAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTC 407
           K ++    ++  L++      +D  +  +S +    EK + LA RC++    +RPSM   
Sbjct: 846 KTKMNKSRSLYDLQE-----LLDTTIIASSGNLKGFEKYVDLALRCVEEEGVNRPSMGEV 904

Query: 408 CEELWGI 411
            +E+  I
Sbjct: 906 VKEIENI 904

BLAST of Carg03347 vs. TAIR 10
Match: AT1G16120.1 (wall associated kinase-like 1 )

HSP 1 Score: 230.3 bits (586), Expect = 3.1e-60
Identity = 138/297 (46.46%), Postives = 182/297 (61.28%), Query Frame = 0

Query: 121 FSIKDLYKATDNFSAANVVGTGSFGTVYKGKLKDGSLVAVKRAKRNANERCLLKAFWNEI 180
           FS K+L KATDNFS   V+G G  GTVYKG L DGS+VAVKR+K    ++  ++ F NEI
Sbjct: 417 FSSKELRKATDNFSIDRVLGQGGQGTVYKGMLVDGSIVAVKRSKVVDEDK--MEEFINEI 476

Query: 181 QALSRIEHLNLVRLYGYLEQGDERIIIVEYVGNGNLREHLDGKRGDVLEIGE-RLDIAID 240
             LS+I H N+V+L G   + +  I++ EY+ NG+L + L  +  D     E RL IAI+
Sbjct: 477 VLLSQINHRNIVKLLGCCLETEVPILVYEYIPNGDLFKRLHDESDDYTMTWEVRLRIAIE 536

Query: 241 VAHAITYLHMYNDAPIIHRDIKATNILITEKLRAKVADFGFARLVAEDSNVTHISTQVKG 300
           +A A+TY+H     PI HRDIK TNIL+ EK RAKV+DFG +R V  D   TH++T V G
Sbjct: 537 IAGALTYMHSAASFPIFHRDIKTTNILLDEKYRAKVSDFGTSRSVTLDQ--THLTTLVAG 596

Query: 301 TAGYLDPEYLRTYQLTEKSDVYSFGVLLVELITGRHPIETKRDVKERVTIRWAMQKLKDG 360
           T GY+DPEY  + Q T KSDVYSFGV+LVELITG  P+   R  + R      ++ +K+ 
Sbjct: 597 TFGYMDPEYFLSSQYTHKSDVYSFGVVLVELITGEKPLSRVRSEEGRGLATHFLEAMKEN 656

Query: 361 EAVIAMDPRLKRTSASTVTMEKTLKLARRCLDPLRPSRPSMKTCCEELWGIRKEYRD 417
             +  +D R+K  S     M    KLAR+CL+    +RP+MK    EL  IR    D
Sbjct: 657 RVIDIIDIRIKDESKLEQVM-AVAKLARKCLNRKGKNRPNMKEVSNELERIRSSPED 708

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022944525.16.5e-254100.00calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita mosc... [more]
XP_022986864.12.7e-25299.13calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita maxi... [more]
XP_023513337.11.0e-25199.13calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 [Cucurbita pepo... [more]
XP_022944526.11.3e-20999.47calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 [Cucurbita mosc... [more]
XP_022986866.11.1e-20898.94calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 [Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
Q9FIL74.5e-10948.82Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Arabidopsis thaliana OX... [more]
Q8VZJ92.5e-9953.64Calmodulin-binding receptor-like cytoplasmic kinase 2 OS=Arabidopsis thaliana OX... [more]
Q9ASQ54.4e-8047.43Calmodulin-binding receptor-like cytoplasmic kinase 3 OS=Arabidopsis thaliana OX... [more]
Q8GZ996.0e-6144.30Leucine-rich repeat receptor protein kinase HPCA1 OS=Arabidopsis thaliana OX=370... [more]
Q9S9M54.3e-5946.46Wall-associated receptor kinase-like 1 OS=Arabidopsis thaliana OX=3702 GN=WAKL1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FYQ33.1e-254100.00calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 OS=Cucurbita mo... [more]
A0A6J1JHA21.3e-25299.13calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X1 OS=Cucurbita ma... [more]
A0A6J1FUN16.2e-21099.47calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 OS=Cucurbita mo... [more]
A0A6J1JCG55.2e-20998.94calmodulin-binding receptor-like cytoplasmic kinase 1 isoform X2 OS=Cucurbita ma... [more]
A0A5D3CVK95.2e-20181.82Calmodulin-binding receptor-like cytoplasmic kinase 1 OS=Cucumis melo var. makuw... [more]
Match NameE-valueIdentityDescription
AT5G58940.13.2e-11048.82calmodulin-binding receptor-like cytoplasmic kinase 1 [more]
AT4G00330.11.8e-10053.64calmodulin-binding receptor-like cytoplasmic kinase 2 [more]
AT2G11520.13.1e-8147.43calmodulin-binding receptor-like cytoplasmic kinase 3 [more]
AT5G49760.14.3e-6244.30Leucine-rich repeat protein kinase family protein [more]
AT1G16120.13.1e-6046.46wall associated kinase-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 133..409
e-value: 7.1E-41
score: 151.8
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 136..408
e-value: 7.5E-53
score: 179.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 133..409
score: 40.032448
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 105..210
e-value: 4.2E-30
score: 105.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 211..428
e-value: 8.5E-56
score: 190.5
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 138..340
e-value: 2.2E-20
score: 71.1
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 132..345
e-value: 2.2E-16
score: 57.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 66..91
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..27
NoneNo IPR availablePANTHERPTHR45974RECEPTOR-LIKE PROTEIN 55coord: 22..436
NoneNo IPR availablePANTHERPTHR45974:SF26CALMODULIN-BINDING RECEPTOR-LIKE CYTOPLASMIC KINASE 1coord: 22..436
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 255..267
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 139..161
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 107..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg03347-RACarg03347-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity