Carg03214 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg03214 vs. NCBI nr
Match: KAG7010652.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 504.6 bits (1298), Expect = 5.1e-139
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60
           KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF
Sbjct: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60

Query: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120
           DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC
Sbjct: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD
Sbjct: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180

Query: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240
           SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE
Sbjct: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240

Query: 241 HLPSSQMFSDENPNACTVM 260
           HLPSSQMFSDENPNACTVM
Sbjct: 241 HLPSSQMFSDENPNACTVM 259

BLAST of Carg03214 vs. NCBI nr
Match: XP_022944012.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita moschata])

HSP 1 Score: 490.0 bits (1260), Expect = 1.3e-134
Identity = 250/259 (96.53%), Postives = 254/259 (98.07%), Query Frame = 0

Query: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60
           KQN  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 4   KQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120
           DASTLREKLADKT KKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC
Sbjct: 64  DASTLREKLADKTNKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD
Sbjct: 124 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 183

Query: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240
           +DDGDGGDKNKSGGEV QE+GGGETDGDRLDYMAVPAPGYEYG+GYGYGYGYGHGGFVGE
Sbjct: 184 NDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 260
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 262

BLAST of Carg03214 vs. NCBI nr
Match: XP_023513163.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 459.9 bits (1182), Expect = 1.4e-125
Identity = 239/259 (92.28%), Postives = 244/259 (94.21%), Query Frame = 0

Query: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60
           KQN  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 4   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120
           DASTLREKLA KTKKKIDIVS+EGKKEKKSKKP    EDEKPKD+E PVTTATLKVELHC
Sbjct: 64  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKP----EDEKPKDEEAPVTTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQ DLVTVKGKMDVKAL+HNLEDKLKRKVAVVVPKKD
Sbjct: 124 QGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKD 183

Query: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240
            DDGDG DKNKSGGEV QENGGGETDGDRLDYMAVPAPGYE  YG+GYGYGYGHGGFVGE
Sbjct: 184 KDDGDGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYE--YGFGYGYGYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 260
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 256

BLAST of Carg03214 vs. NCBI nr
Match: XP_022985576.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita maxima])

HSP 1 Score: 449.5 bits (1155), Expect = 1.9e-122
Identity = 233/259 (89.96%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60
           K+N  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 4   KKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120
           DAS+LREKLADKTKKKIDIVS EGKKEK SKKP    EDEKPKD+E P TTATLKVELHC
Sbjct: 64  DASSLREKLADKTKKKIDIVSLEGKKEKGSKKP----EDEKPKDEEAPATTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180
           QGC+ERIYKVVSRTKGVEDMAME QKDLVTVKGKMDVKAL+HNLE+KLKRKVAVVVPKKD
Sbjct: 124 QGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKD 183

Query: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240
            DDGDGGDKNKSGGEVAQ++GGGETDGDRLDYMAVPAPGYE  YG+GYGY YGHGGFVGE
Sbjct: 184 KDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYE--YGFGYGYVYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 260
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 256

BLAST of Carg03214 vs. NCBI nr
Match: KAG6570811.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 416.0 bits (1068), Expect = 2.4e-112
Identity = 215/220 (97.73%), Postives = 218/220 (99.09%), Query Frame = 0

Query: 40  QGVERVRVDWEANKLTVIGKFDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAED 99
           +GVERVR DWEANKLTVIGKFDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAED
Sbjct: 96  EGVERVRADWEANKLTVIGKFDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAED 155

Query: 100 EKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKA 159
           EKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKA
Sbjct: 156 EKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKA 215

Query: 160 LVHNLEDKLKRKVAVVVPKKDSDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPG 219
           LVHNLEDKLKRKVAVVVPKKDSDDGDGGDKNKSGGEV QE+GGGETDGDRLDYMAVPAPG
Sbjct: 216 LVHNLEDKLKRKVAVVVPKKDSDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPG 275

Query: 220 YEYGYGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 260
           YEYG+GYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM
Sbjct: 276 YEYGFGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 315

BLAST of Carg03214 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.1e-51
Identity = 132/281 (46.98%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 1   KQNSGHGSDKQE-DSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGK 60
           K+  G    K E  S T V  +DMHCEGCA+++ +CVR  QGVE V+ +    KLTV G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA 70

Query: 61  FDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEK------------PKDKEI 120
            D   LREKL +KTKKK+D+VS + KKEK+ +  ++  ED+K             K KE 
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 121 PVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDK 180
           PVTTA LK+  HCQGC+ +I K V++TKGV  + M+++K+L+TVKG MDVK LV  L +K
Sbjct: 131 PVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEK 190

Query: 181 LKRKVAVVVPKKDSDDGDG---GDKNKSGG------EVAQENGGGETDGDRLDYMAVPAP 240
           LKR V +V PKK+ D  +G   G+K K GG      E     GGGE   + ++YMA   P
Sbjct: 191 LKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAA-QP 250

Query: 241 GYEYGYGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 260
            Y YGY  G  YGY     +  H P  Q+FSDENPNAC VM
Sbjct: 251 AYGYGYYPGGPYGYP----IQAHAP--QIFSDENPNACVVM 283

BLAST of Carg03214 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 137.1 bits (344), Expect = 2.8e-31
Identity = 111/334 (33.23%), Postives = 151/334 (45.21%), Query Frame = 0

Query: 18  VFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLREKLADKTKKKI 77
           V+ +DMHCEGCA K++R V+   GV+ V  D   NKL V+GK D   L+EKL +KTK+K+
Sbjct: 53  VYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVVGKIDPVKLQEKLEEKTKRKV 112

Query: 78  DIVSSEGK---------KEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHCQGCVERIY 137
            + +   K          EKK+   D+EA    P       +   LK+ LHC+GC+++I 
Sbjct: 113 VLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIK 172

Query: 138 KVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKDSDD----- 197
           K++ + KGVE +A++  KD+VTVKG +DVK LV  L  KLKR V  +VP K  D      
Sbjct: 173 KIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLLTKKLKRTVEPLVPAKKDDGAAENK 232

Query: 198 -----------------------------------GDGGDKNKSGGEVAQE----NGGGE 257
                                              GDGG+K K GG+  ++      GGE
Sbjct: 233 KTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGGE 292

Query: 258 TDGD------------RLDYMAVPA---------PGYEYGYGY---------------GY 260
              D            ++DY    A          G+ YG  Y               G 
Sbjct: 293 KKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQEGHVYGQSYSMTGQNYPVGGQSYPGS 352

BLAST of Carg03214 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.0e-29
Identity = 122/380 (32.11%), Postives = 168/380 (44.21%), Query Frame = 0

Query: 7   GSDKQEDS--TTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDAST 66
           G  K  D   TT V  +DMHCEGC  K++R  +  +GVE V++D+++NKLTVIG  D   
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVE 73

Query: 67  LREKLADKTKKKIDIVS-----------SEGKKEKKS----------------KKPDEEA 126
           +R+K+ADK K+ +++VS           S G  EKK                 +KP E+ 
Sbjct: 74  VRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKK 133

Query: 127 EDEKPKDKEIPV--------TTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLV 186
           E++K ++ E           +T  LK +LHC+GC  +I ++V++ KGV  +A++  KDLV
Sbjct: 134 EEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLV 193

Query: 187 TVKGKMDVKALVHNLEDKLKRKVAVVVPKKD----------------SDDGDGGDKNK-- 246
            VKG +DVK L   L +KLKR V VV  KKD                  D   G+K +  
Sbjct: 194 IVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEIK 253

Query: 247 --------SGGEVAQE--------NGGGETDGDRLD--------YMAVPAPGYEY----- 260
                    GGE  +E         GGG  DG  +D        Y   P P Y Y     
Sbjct: 254 DVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGYPPQPMYYYPEGQV 313

BLAST of Carg03214 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 6.4e-28
Identity = 95/259 (36.68%), Postives = 140/259 (54.05%), Query Frame = 0

Query: 10  KQEDSTTA----VFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIG-KFDAST 69
           K++++TT+    V  ID HC+GC  ++ R  R+++GVE VR D ++NKLT+IG   D   
Sbjct: 20  KKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPVK 79

Query: 70  LREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKE---IPVTTATLKVELHCQ 129
           + EKL  K+KKK++++S + KK+ K      E  ++K  DK    + VTT  LKV   C 
Sbjct: 80  IAEKLQKKSKKKVELISPKPKKDTK------ENNEKKANDKTQTVVAVTTVVLKVNCSCD 139

Query: 130 GCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVV-VPKKD 189
           GC++RI K VS TKGV  + M+++K+ VTV G MD+K++  NL+ KLK+ V VV   KK 
Sbjct: 140 GCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKKKK 199

Query: 190 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 249
             D D  + N   G   Q                 P  G  +G G    Y +  G     
Sbjct: 200 KKDKDNAEVNTKVGSPCQ-----------------PGNGCTHGIG---PYRFMEG----- 245

Query: 250 HLPSSQMFSDENPNACTVM 260
             P +  FS+E+ + C+VM
Sbjct: 260 --PMTGFFSEEDQSYCSVM 245

BLAST of Carg03214 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.0e-17
Identity = 79/272 (29.04%), Postives = 120/272 (44.12%), Query Frame = 0

Query: 18  VFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLREKLADKTKKKI 77
           +  +D+HC GCA K+ R + +I+GVE V +D   N++T+ G  D   +  K+  KTK+  
Sbjct: 59  ILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNENQVTIKGVLDPQAVCNKIKKKTKRMA 118

Query: 78  DIVSSEGKKEKKSKKPDEEAEDEKP---KDKEIPVTTATLKVELHCQGCVERIYKVVSRT 137
            ++         S  P  E E   P         +TT  L V +HCQ C +++ K + + 
Sbjct: 119 KVL---------SPLPAAEGEPLPPIITSQVSGGLTTVELSVNMHCQACADQLKKKILKM 178

Query: 138 KGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVV-------------VPKKDS 197
           +GV+    E     V V G MD + LV  +  + K++  +V               +K  
Sbjct: 179 RGVQTTVTEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARIVPQPDPEPEAPAAAQEEKKE 238

Query: 198 DDGDGGDK-----------NKSGGEVAQENGGGE----TDGDRLDYMAVPAPGYEYGYGY 257
           + G+G +K            K  GE   E GGGE    T+ +R D         E   G 
Sbjct: 239 ESGEGNEKPPETGEEKEEEKKKEGEENGEEGGGEEAAATEEERRDNEMTAMAQEE---GM 298

Query: 258 GYGYGYGHGGFVGEHLPSSQMFSDENPNACTV 259
                Y    +V E +P  Q+FSDENPNAC +
Sbjct: 299 KRMMYYYQPSYVIERIPPPQLFSDENPNACCI 318

BLAST of Carg03214 vs. ExPASy TrEMBL
Match: A0A6J1FUL5 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111448569 PE=4 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 6.3e-135
Identity = 250/259 (96.53%), Postives = 254/259 (98.07%), Query Frame = 0

Query: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60
           KQN  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 4   KQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120
           DASTLREKLADKT KKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC
Sbjct: 64  DASTLREKLADKTNKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD
Sbjct: 124 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 183

Query: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240
           +DDGDGGDKNKSGGEV QE+GGGETDGDRLDYMAVPAPGYEYG+GYGYGYGYGHGGFVGE
Sbjct: 184 NDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 260
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 262

BLAST of Carg03214 vs. ExPASy TrEMBL
Match: A0A6J1J8L7 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111483606 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 9.4e-123
Identity = 233/259 (89.96%), Postives = 243/259 (93.82%), Query Frame = 0

Query: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60
           K+N  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 4   KKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120
           DAS+LREKLADKTKKKIDIVS EGKKEK SKKP    EDEKPKD+E P TTATLKVELHC
Sbjct: 64  DASSLREKLADKTKKKIDIVSLEGKKEKGSKKP----EDEKPKDEEAPATTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180
           QGC+ERIYKVVSRTKGVEDMAME QKDLVTVKGKMDVKAL+HNLE+KLKRKVAVVVPKKD
Sbjct: 124 QGCIERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKD 183

Query: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240
            DDGDGGDKNKSGGEVAQ++GGGETDGDRLDYMAVPAPGYE  YG+GYGY YGHGGFVGE
Sbjct: 184 KDDGDGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYE--YGFGYGYVYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 260
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 256

BLAST of Carg03214 vs. ExPASy TrEMBL
Match: A0A1S3CP34 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103502993 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 1.0e-100
Identity = 206/270 (76.30%), Postives = 230/270 (85.19%), Query Frame = 0

Query: 7   GSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLR 66
           G  K +D TTAVF ID+HCEGCANKLR+ VR+I GV RVR DWEANKLTVIGKFDA+ LR
Sbjct: 2   GEKKNDDITTAVFKIDIHCEGCANKLRKSVREIPGVARVRADWEANKLTVIGKFDAAKLR 61

Query: 67  EKLADKTKKKIDIVSSEGKKEKKS------KKPDEEAEDEK-PKDKEIPVTTATLKVELH 126
           + LADKTKKKIDIVSSEGKKEK+S      +KPD++ ED+K PKDKEIPVTTATLKVELH
Sbjct: 62  DNLADKTKKKIDIVSSEGKKEKESAKKQDDEKPDKKTEDKKQPKDKEIPVTTATLKVELH 121

Query: 127 CQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKK 186
           CQGC+E+IYKVVSRTKGVEDMA+ERQKDLV VKGKMDVKAL+ NLE+KLKRKVAVVVPKK
Sbjct: 122 CQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKK 181

Query: 187 DSDD----GDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHG 246
           D DD    GDGGDKNK+GGEVAQE  GG  +G+RLDY+AVP PG  YGYGYGYGYGYG+G
Sbjct: 182 DKDDGAKGGDGGDKNKTGGEVAQE--GGAMEGNRLDYVAVPVPG--YGYGYGYGYGYGNG 241

Query: 247 GFVGEHLP------SSQMFSDENPNACTVM 260
           GFVG+HLP      S QMFSDENPNAC++M
Sbjct: 242 GFVGQHLPQPQPLLSPQMFSDENPNACSLM 267

BLAST of Carg03214 vs. ExPASy TrEMBL
Match: A0A0A0KDD7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G147620 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.2e-98
Identity = 203/270 (75.19%), Postives = 227/270 (84.07%), Query Frame = 0

Query: 7   GSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLR 66
           G  K +D+TTAVF ID+HCEGCANKLRR VRQI GV RVR DWEANKLTVIGKFD + LR
Sbjct: 2   GEKKNDDTTTAVFKIDIHCEGCANKLRRSVRQIPGVSRVRADWEANKLTVIGKFDPAKLR 61

Query: 67  EKLADKTKKKIDIVSSEGKKEKKS------KKPDEEAEDEK-PKDKEIPVTTATLKVELH 126
           + LADK  KKIDIVSSE KKEK+S      +KPD++ ED+K PKDKEIPVTTATLKVELH
Sbjct: 62  DYLADKETKKIDIVSSESKKEKESTKKQDDEKPDKKTEDKKQPKDKEIPVTTATLKVELH 121

Query: 127 CQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKK 186
           CQGC+E+IYKVVSRTKGVEDMA+ERQKDLV VKGKMDVKAL+ NLE+KLKRKVAVVVPKK
Sbjct: 122 CQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKK 181

Query: 187 DSDD----GDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHG 246
           D D+    GDGGDKNK+GGEVAQ  GGG  +G+RLDY+AVP PG  YGYGYGYGYGYG+G
Sbjct: 182 DKDEGAKGGDGGDKNKTGGEVAQ--GGGAMEGNRLDYVAVPVPG--YGYGYGYGYGYGNG 241

Query: 247 GFVGEHLP------SSQMFSDENPNACTVM 260
           GFVG+H+P      S QMFSDENPNAC+VM
Sbjct: 242 GFVGQHMPPAQPLISPQMFSDENPNACSVM 267

BLAST of Carg03214 vs. ExPASy TrEMBL
Match: A0A6J1H2S6 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111459839 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.7e-95
Identity = 195/264 (73.86%), Postives = 214/264 (81.06%), Query Frame = 0

Query: 7   GSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLR 66
           G  K ED+TTAVF IDMHCEGCANK+R+CVR+I+GVER R DWEANKLTVIGKFD + LR
Sbjct: 2   GEKKNEDTTTAVFKIDMHCEGCANKIRKCVREIEGVERARADWEANKLTVIGKFDPAKLR 61

Query: 67  EKLADKTKKKIDIVSSEGKKEKKSKKPDEEA------EDEKPKDKEIPVTTATLKVELHC 126
           EKLADKTKKKIDIVSSE KKEK+SKKPDE A      E++K KDKE PVTT TLKVELHC
Sbjct: 62  EKLADKTKKKIDIVSSENKKEKESKKPDEPAVGDKKPEEKKSKDKETPVTTVTLKVELHC 121

Query: 127 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 186
           QGC+E+IYKVV+RTKGVEDMA+ERQKDLV VKGKMDVKAL+ NLE+KL+RKVAVVVPKKD
Sbjct: 122 QGCIEKIYKVVARTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLRRKVAVVVPKKD 181

Query: 187 SDD----GDGGDKNKSGGEVAQ-ENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHG 246
            DD    GD  DKNKSGG+ AQ  NG G  DG RLD+MAVP  GY YGYGYG        
Sbjct: 182 KDDEAKGGDSSDKNKSGGDAAQPANGSGGGDGHRLDHMAVPVLGYGYGYGYG-------- 241

Query: 247 GFVGEHLPSSQMFSDENPNACTVM 260
              GEHLPS QMFSDENPNAC+VM
Sbjct: 242 ---GEHLPSVQMFSDENPNACSVM 254

BLAST of Carg03214 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 203.0 bits (515), Expect = 2.9e-52
Identity = 132/281 (46.98%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 1   KQNSGHGSDKQE-DSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGK 60
           K+  G    K E  S T V  +DMHCEGCA+++ +CVR  QGVE V+ +    KLTV G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA 70

Query: 61  FDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEK------------PKDKEI 120
            D   LREKL +KTKKK+D+VS + KKEK+ +  ++  ED+K             K KE 
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 121 PVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDK 180
           PVTTA LK+  HCQGC+ +I K V++TKGV  + M+++K+L+TVKG MDVK LV  L +K
Sbjct: 131 PVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEK 190

Query: 181 LKRKVAVVVPKKDSDDGDG---GDKNKSGG------EVAQENGGGETDGDRLDYMAVPAP 240
           LKR V +V PKK+ D  +G   G+K K GG      E     GGGE   + ++YMA   P
Sbjct: 191 LKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAA-QP 250

Query: 241 GYEYGYGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 260
            Y YGY  G  YGY     +  H P  Q+FSDENPNAC VM
Sbjct: 251 AYGYGYYPGGPYGYP----IQAHAP--QIFSDENPNACVVM 283

BLAST of Carg03214 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 8.6e-52
Identity = 131/281 (46.62%), Postives = 170/281 (60.50%), Query Frame = 0

Query: 1   KQNSGHGSDKQE-DSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGK 60
           K+  G    K E  S T V  +DMHCEGCA+++ +CVR  QGVE V+ +    KLTV G 
Sbjct: 11  KKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGA 70

Query: 61  FDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEK------------PKDKEI 120
            D   LREKL +KTKKK+D+VS + KKEK+ +  ++  ED+K             K KE 
Sbjct: 71  LDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKET 130

Query: 121 PVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDK 180
           PVTTA LK+  HCQGC+ +I K V++TKGV  + M+++K+L+TVKG MDVK LV  L +K
Sbjct: 131 PVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEK 190

Query: 181 LKRKVAVVVPKKDSDDGDG---GDKNKSGG------EVAQENGGGETDGDRLDYMAVPAP 240
           LKR V +V PKK+ D  +G   G+K K GG      E     GGGE   + ++YMA   P
Sbjct: 191 LKRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAA-QP 250

Query: 241 GYEYGYGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 260
            Y YGY  G  YGY     +  H P  Q+FSDENPNAC V+
Sbjct: 251 AYGYGYYPGGPYGYP----IQAHAP--QIFSDENPNACVVI 283

BLAST of Carg03214 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.0e-32
Identity = 111/334 (33.23%), Postives = 151/334 (45.21%), Query Frame = 0

Query: 18  VFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLREKLADKTKKKI 77
           V+ +DMHCEGCA K++R V+   GV+ V  D   NKL V+GK D   L+EKL +KTK+K+
Sbjct: 53  VYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVVGKIDPVKLQEKLEEKTKRKV 112

Query: 78  DIVSSEGK---------KEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHCQGCVERIY 137
            + +   K          EKK+   D+EA    P       +   LK+ LHC+GC+++I 
Sbjct: 113 VLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIK 172

Query: 138 KVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKDSDD----- 197
           K++ + KGVE +A++  KD+VTVKG +DVK LV  L  KLKR V  +VP K  D      
Sbjct: 173 KIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLLTKKLKRTVEPLVPAKKDDGAAENK 232

Query: 198 -----------------------------------GDGGDKNKSGGEVAQE----NGGGE 257
                                              GDGG+K K GG+  ++      GGE
Sbjct: 233 KTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGGE 292

Query: 258 TDGD------------RLDYMAVPA---------PGYEYGYGY---------------GY 260
              D            ++DY    A          G+ YG  Y               G 
Sbjct: 293 KKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQEGHVYGQSYSMTGQNYPVGGQSYPGS 352

BLAST of Carg03214 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.4e-30
Identity = 122/380 (32.11%), Postives = 168/380 (44.21%), Query Frame = 0

Query: 7   GSDKQEDS--TTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDAST 66
           G  K  D   TT V  +DMHCEGC  K++R  +  +GVE V++D+++NKLTVIG  D   
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVE 73

Query: 67  LREKLADKTKKKIDIVS-----------SEGKKEKKS----------------KKPDEEA 126
           +R+K+ADK K+ +++VS           S G  EKK                 +KP E+ 
Sbjct: 74  VRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKK 133

Query: 127 EDEKPKDKEIPV--------TTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLV 186
           E++K ++ E           +T  LK +LHC+GC  +I ++V++ KGV  +A++  KDLV
Sbjct: 134 EEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLV 193

Query: 187 TVKGKMDVKALVHNLEDKLKRKVAVVVPKKD----------------SDDGDGGDKNK-- 246
            VKG +DVK L   L +KLKR V VV  KKD                  D   G+K +  
Sbjct: 194 IVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEIK 253

Query: 247 --------SGGEVAQE--------NGGGETDGDRLD--------YMAVPAPGYEY----- 260
                    GGE  +E         GGG  DG  +D        Y   P P Y Y     
Sbjct: 254 DVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGYPPQPMYYYPEGQV 313

BLAST of Carg03214 vs. TAIR 10
Match: AT5G03380.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 125.6 bits (314), Expect = 6.0e-29
Identity = 116/364 (31.87%), Postives = 162/364 (44.51%), Query Frame = 0

Query: 21  IDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKFDASTLREKLADKTKKKIDIV 80
           +DMHCEGC  K++R  +  +GVE V++D+++NKLTVIG  D   +R+K+ADK K+ +++V
Sbjct: 3   LDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVEVRDKVADKIKRPVELV 62

Query: 81  S-----------SEGKKEKKS----------------KKPDEEAEDEKPKDKEIPV---- 140
           S           S G  EKK                 +KP E+ E++K ++ E       
Sbjct: 63  STVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPP 122

Query: 141 ----TTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLE 200
               +T  LK +LHC+GC  +I ++V++ KGV  +A++  KDLV VKG +DVK L   L 
Sbjct: 123 PPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLN 182

Query: 201 DKLKRKVAVVVPKKD----------------SDDGDGGDKNK----------SGGEVAQE 260
           +KLKR V VV  KKD                  D   G+K +           GGE  +E
Sbjct: 183 EKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEIKDVGEKKVDGGGEKKKE 242