Carg03187 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03187
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Phospholipid:diacylglycerol acyltransferase
Location	Carg_Chr20: 2665557 .. 2666428 (-)
RNA-Seq Expression	Carg03187
Synteny	Carg03187

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTACGTCAACCCTGTTAAGAATTCTTCTATGGGGATCCGAACCATTGATGATGCACATAAATCAGAGGATAAACCTAATGTTGTTGTTGCATATGTGAAGAAAATGCTGCAAAAGAAGCAAGATAAGGACAGGAAGAAGAAAAGGAGCAAGAACATCAATAAGGGGCAATCAAGAGAATGGAAGTGCTTAGATAACTGCTGTTGGATTATTGGATATATGTGTACATTTTGGTGGCTGATGTTTTTCGTCTACCATTATTTGCCTCTTACATTACCTGGCTTTCCAGCGATTGACTCTCCGGGGGCTGTTTTAAAGCGAGATGGCGTATCAGGTCTTCACCCAGTCGTTTTAGTGCCCGGCATTGTCACCGGCGGGCTGGAGCTCTGGGATGGCAAGCCCTGCGCCAAGGGACTGTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGGTTAGCGAATTCCAGATGAATTTTACATTCTGAAGCTTTTGGATTGATACTTGAAGTACCCATTTGAGAATTTGAATTCAAACTTCCAGACCATTATGCTGGTTGGAGCACCTGTCTCTGGATAATGAAACAGGGCTTGACCCACCAGGCATTCGAATCCGGCCAGTCGAAGGCCTTGTGGCCGCTGATTATTTCACCCAAGGCTACTTCGTTTGGGCTGTTCTCATAGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTACATATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCAACGTGACCAAAAACACTCTCTTCTTCTTCTTCCAATCAAATTATACATTTGATCATTGA

mRNA sequence

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTACGTCAACCCTGTTAAGAATTCTTCTATGGGGATCCGAACCATTGATGATGCACATAAATCAGAGGATAAACCTAATGTTGTTGTTGCATATGTGAAGAAAATGCTGCAAAAGAAGCAAGATAAGGACAGGAAGAAGAAAAGGAGCAAGAACATCAATAAGGGGCAATCAAGAGAATGGAAGTGCTTAGATAACTGCTGTTGGATTATTGGATATATGTGTACATTTTGGTGGCTGATGTTTTTCGTCTACCATTATTTGCCTCTTACATTACCTGGCTTTCCAGCGATTGACTCTCCGGGGGCTGTTTTAAAGCGAGATGGCGTATCAGGTCTTCACCCAGTCGTTTTAGTGCCCGGCATTGTCACCGGCGGGCTGGAGCTCTGGGATGGCAAGCCCTGCGCCAAGGGACTGTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCATTATGCTGGTTGGAGCACCTGTCTCTGGATAATGAAACAGGGCTTGACCCACCAGGCATTCGAATCCGGCCAGTCGAAGGCCTTGTGGCCGCTGATTATTTCACCCAAGGCTACTTCGTTTGGGCTGTTCTCATAGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTACATATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCAACGTGACCAAAAACACTCTCTTCTTCTTCTTCCAATCAAATTATACATTTGATCATTGA

Coding sequence (CDS)

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTACGTCAACCCTGTTAAGAATTCTTCTATGGGGATCCGAACCATTGATGATGCACATAAATCAGAGGATAAACCTAATGTTGTTGTTGCATATGTGAAGAAAATGCTGCAAAAGAAGCAAGATAAGGACAGGAAGAAGAAAAGGAGCAAGAACATCAATAAGGGGCAATCAAGAGAATGGAAGTGCTTAGATAACTGCTGTTGGATTATTGGATATATGTGTACATTTTGGTGGCTGATGTTTTTCGTCTACCATTATTTGCCTCTTACATTACCTGGCTTTCCAGCGATTGACTCTCCGGGGGCTGTTTTAAAGCGAGATGGCGTATCAGGTCTTCACCCAGTCGTTTTAGTGCCCGGCATTGTCACCGGCGGGCTGGAGCTCTGGGATGGCAAGCCCTGCGCCAAGGGACTGTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCATTATGCTGGTTGGAGCACCTGTCTCTGGATAATGAAACAGGGCTTGACCCACCAGGCATTCGAATCCGGCCAGTCGAAGGCCTTGTGGCCGCTGATTATTTCACCCAAGGCTACTTCGTTTGGGCTGTTCTCATAGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTACATATGGCTGCATATGATTGGAGGATCGCCTTCCAAAACACAGAGGTATGCAACGTGACCAAAAACACTCTCTTCTTCTTCTTCCAATCAAATTATACATTTGATCATTGA

Protein sequence

MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRSKNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVCNVTKNTLFFFFQSNYTFDH

Homology

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BLAST of Carg03187 vs. NCBI nr
Match: KAG7010679.1 (putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 549.7 bits (1415), Expect = 1.4e-152
Identity = 260/260 (100.00%), Postives = 260/260 (100.00%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

Query: 241 CNVTKNTLFFFFQSNYTFDH 261
           CNVTKNTLFFFFQSNYTFDH
Sbjct: 241 CNVTKNTLFFFFQSNYTFDH 260

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BLAST of Carg03187 vs. NCBI nr
Match: KAG6570836.1 (putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 507.3 bits (1305), Expect = 7.9e-140
Identity = 240/240 (100.00%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

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BLAST of Carg03187 vs. NCBI nr
Match: XP_022944139.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata])

HSP 1 Score: 504.6 bits (1298), Expect = 5.1e-139
Identity = 238/240 (99.17%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGI+TIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKR+
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

[Close]

BLAST of Carg03187 vs. NCBI nr
Match: XP_022986276.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 504.6 bits (1298), Expect = 5.1e-139
Identity = 238/240 (99.17%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGI+TIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKR+
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

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BLAST of Carg03187 vs. NCBI nr
Match: XP_023512597.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497.7 bits (1280), Expect = 6.2e-137
Identity = 234/240 (97.50%), Postives = 237/240 (98.75%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGI++IDDAHKSEDKPNVVVAYVKKMLQKKQD DRKKKR+
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQSIDDAHKSEDKPNVVVAYVKKMLQKKQDNDRKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPG VLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGTVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFT GYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTHGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

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BLAST of Carg03187 vs. ExPASy Swiss-Prot
Match: Q9FYC7 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 4.6e-74
Identity = 126/188 (67.02%), Postives = 150/188 (79.79%), Query Frame = 0


Query: 56  KKKRSKNINKGQSR---EWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPG 115
           K K+S  + K + R      C+D+CCW+IGY+CT WWL+ F+YH +P+      A +SPG
Sbjct: 21  KPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVPVPAM-LQAPESPG 80

Query: 116 AVLKRDGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEH 175
             L RDGV   HPV+LVPGIVTGGLELW+G+PCA+GLFRKRLWG SF+E L+RPLCWLEH
Sbjct: 81  TRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASFSEILRRPLCWLEH 140

Query: 176 LSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWR 235
           LSLD+ETGLDP GIR+R V GLVAADYF   YF WAVLIENLAKIGY+GKNLHMA+YDWR
Sbjct: 141 LSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYDWR 200

Query: 236 IAFQNTEV 241
           ++F NTEV
Sbjct: 201 LSFHNTEV 207

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BLAST of Carg03187 vs. ExPASy Swiss-Prot
Match: Q9FNA9 (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 6.8e-70
Identity = 121/221 (54.75%), Postives = 153/221 (69.23%), Query Frame = 0


Query: 31  KSEDKPNVVVAYVKKMLQKKQDKDRKKKRSKNINKGQSREWKCLDNCCWIIGYMCTFWWL 90
           K  +KP+   +      +  Q K  +  +S +       +W C+D+CCW IG +C  WW 
Sbjct: 8   KPTEKPSTPPSEEVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWF 67

Query: 91  MFFVYHYLPLTLPGF-------PAIDSPGAVLKRDGVSGLHPVVLVPGIVTGGLELWDGK 150
           + F+Y+ +P + P +       P  D PG  LK++G+   HPVV +PGIVTGGLELW+GK
Sbjct: 68  LLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGK 127

Query: 151 PCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQG 210
            CA GLFRKRLWGG+F E  KRPLCW+EH+SLDNETGLDP GIR+R V GLVAADYF  G
Sbjct: 128 QCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPG 187

Query: 211 YFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVCNVT 245
           YFVWAVLI NLA IGY+ KN++MAAYDWR++FQNTEV + T
Sbjct: 188 YFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQT 228

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BLAST of Carg03187 vs. ExPASy Swiss-Prot
Match: O94680 (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=plh1 PE=1 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 2.5e-32
Identity = 60/130 (46.15%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 112 GAVLKRDGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWG--GSFTETLKRPLCW 171
           G  +  +G    HPV++VPG+++ GLE W    C+   FRKRLWG             CW
Sbjct: 134 GLDMYNEGYRSDHPVIMVPGVISSGLESWSFNNCSIPYFRKRLWGSWSMLKAMFLDKQCW 193

Query: 172 LEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAY 231
           LEHL LD +TGLDP GI++R  +G  AAD+F  GY++W+ +IENLA IGY+  N+  A+Y
Sbjct: 194 LEHLMLDKKTGLDPKGIKLRAAQGFEAADFFITGYWIWSKVIENLAAIGYEPNNMLSASY 253

Query: 232 DWRIAFQNTE 240
           DWR+++ N E
Sbjct: 254 DWRLSYANLE 263

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BLAST of Carg03187 vs. ExPASy Swiss-Prot
Match: P40345 (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LRO1 PE=1 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 3.2e-27
Identity = 56/135 (41.48%), Postives = 81/135 (60.00%), Query Frame = 0


Query: 112 GAVLKRD-GVSGLHPVVLVPGIVTGGLELW----DGKPCAKGLFRKRLWGGSF--TETLK 171
           G  L RD  +   HPVV+VPG+++ G+E W    D +  +   FRKRLWG  +     + 
Sbjct: 160 GKQLLRDYNIEAKHPVVMVPGVISTGIESWGVIGDDECDSSAHFRKRLWGSFYMLRTMVM 219

Query: 172 RPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNL 231
             +CWL+H+ LD ETGLDPP   +R  +G  + DYF  GY++W  + +NL  IGY+   +
Sbjct: 220 DKVCWLKHVMLDPETGLDPPNFTLRAAQGFESTDYFIAGYWIWNKVFQNLGVIGYEPNKM 279

Query: 232 HMAAYDWRIAFQNTE 240
             AAYDWR+A+ + E
Sbjct: 280 TSAAYDWRLAYLDLE 294

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BLAST of Carg03187 vs. ExPASy TrEMBL
Match: A0A6J1JAN3 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484072 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.5e-139
Identity = 238/240 (99.17%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGI+TIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKR+
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

[Close]

BLAST of Carg03187 vs. ExPASy TrEMBL
Match: A0A6J1FW53 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=3662 GN=LOC111448684 PE=4 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 2.5e-139
Identity = 238/240 (99.17%), Postives = 240/240 (100.00%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLYYVNPVKNSSMGI+TIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKR+
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

[Close]

BLAST of Carg03187 vs. ExPASy TrEMBL
Match: A0A5A7SR19 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00090 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 4.8e-127
Identity = 214/240 (89.17%), Postives = 234/240 (97.50%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKSEDK NV+VAYVKK++QKKQDKD K+KR+
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KN N+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL++PGFPAIDSPGAVLKR+G+
Sbjct: 61  KNTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGI 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 239

[Close]

BLAST of Carg03187 vs. ExPASy TrEMBL
Match: A0A1S3C9K8 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497986 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 4.8e-127
Identity = 214/240 (89.17%), Postives = 234/240 (97.50%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKSEDK NV+VAYVKK++QKKQDKD K+KR+
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRN 173

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KN N+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL++PGFPAIDSPGAVLKR+G+
Sbjct: 174 KNTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGI 233

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 234 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 293

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 294 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 352

[Close]

BLAST of Carg03187 vs. ExPASy TrEMBL
Match: A0A0A0KGM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.8e-126
Identity = 213/240 (88.75%), Postives = 233/240 (97.08%), Query Frame = 0


Query: 1   MASVLRLRKLYYVNPVKNSSMGIRTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRS 60
           MASVLRLRKLY+VNPVK+SSMG+++I +AHKS++K NV+VAYVK ++QKKQDKD KKKR+
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSI-EAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRN 60

Query: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120
           KNIN+GQSREWKCLDNCCWIIGY+CTFWWLMFF+YHYLPL+LPGFP IDSPGAVLKR+G+
Sbjct: 61  KNINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGI 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SG HPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 239

[Close]

BLAST of Carg03187 vs. TAIR 10
Match: AT3G44830.1 (Lecithin:cholesterol acyltransferase family protein )

HSP 1 Score: 279.3 bits (713), Expect = 3.2e-75
Identity = 126/188 (67.02%), Postives = 150/188 (79.79%), Query Frame = 0


Query: 56  KKKRSKNINKGQSR---EWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPG 115
           K K+S  + K + R      C+D+CCW+IGY+CT WWL+ F+YH +P+      A +SPG
Sbjct: 21  KPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVPVPAM-LQAPESPG 80

Query: 116 AVLKRDGVSGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEH 175
             L RDGV   HPV+LVPGIVTGGLELW+G+PCA+GLFRKRLWG SF+E L+RPLCWLEH
Sbjct: 81  TRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASFSEILRRPLCWLEH 140

Query: 176 LSLDNETGLDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWR 235
           LSLD+ETGLDP GIR+R V GLVAADYF   YF WAVLIENLAKIGY+GKNLHMA+YDWR
Sbjct: 141 LSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYDWR 200

Query: 236 IAFQNTEV 241
           ++F NTEV
Sbjct: 201 LSFHNTEV 207

[Close]

BLAST of Carg03187 vs. TAIR 10
Match: AT5G13640.1 (phospholipid:diacylglycerol acyltransferase )

HSP 1 Score: 265.4 bits (677), Expect = 4.9e-71
Identity = 121/221 (54.75%), Postives = 153/221 (69.23%), Query Frame = 0


Query: 31  KSEDKPNVVVAYVKKMLQKKQDKDRKKKRSKNINKGQSREWKCLDNCCWIIGYMCTFWWL 90
           K  +KP+   +      +  Q K  +  +S +       +W C+D+CCW IG +C  WW 
Sbjct: 8   KPTEKPSTPPSEEVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWF 67

Query: 91  MFFVYHYLPLTLPGF-------PAIDSPGAVLKRDGVSGLHPVVLVPGIVTGGLELWDGK 150
           + F+Y+ +P + P +       P  D PG  LK++G+   HPVV +PGIVTGGLELW+GK
Sbjct: 68  LLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGK 127

Query: 151 PCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRIRPVEGLVAADYFTQG 210
            CA GLFRKRLWGG+F E  KRPLCW+EH+SLDNETGLDP GIR+R V GLVAADYF  G
Sbjct: 128 QCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPG 187

Query: 211 YFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVCNVT 245
           YFVWAVLI NLA IGY+ KN++MAAYDWR++FQNTEV + T
Sbjct: 188 YFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQT 228

The following BLAST results are available for this feature:

BLAST of Carg03187 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010679.1	1.4e-152	100.00	putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyr...	[more]
KAG6570836.1	7.9e-140	100.00	putative phospholipid:diacylglycerol acyltransferase 2, partial [Cucurbita argyr...	[more]
XP_022944139.1	5.1e-139	99.17	putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita moschata]	[more]
XP_022986276.1	5.1e-139	99.17	putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima]	[more]
XP_023512597.1	6.2e-137	97.50	putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita pepo subsp. pe...	[more]

BLAST of Carg03187 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYC7	4.6e-74	67.02	Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana O...	[more]
Q9FNA9	6.8e-70	54.75	Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
O94680	2.5e-32	46.15	Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain...	[more]
P40345	3.2e-27	41.48	Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ...	[more]

BLAST of Carg03187 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JAN3	2.5e-139	99.17	putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=36...	[more]
A0A6J1FW53	2.5e-139	99.17	putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=...	[more]
A0A5A7SR19	4.8e-127	89.17	Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. maku...	[more]
A0A1S3C9K8	4.8e-127	89.17	putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 G...	[more]
A0A0A0KGM0	1.8e-126	88.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1	[more]

BLAST of Carg03187 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G44830.1	3.2e-75	67.02	Lecithin:cholesterol acyltransferase family protein	[more]
AT5G13640.1	4.9e-71	54.75	phospholipid:diacylglycerol acyltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 176..254 e-value: 3.5E-6 score: 28.8
IPR003386	Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferase	PFAM	PF02450	LCAT	coord: 150..237 e-value: 7.0E-18 score: 65.0
None	No IPR available	PANTHER	PTHR11440	LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED	coord: 44..241
None	No IPR available	PANTHER	PTHR11440:SF87	PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATED	coord: 44..241

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03187-RA	Carg03187-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity