Carg03174 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03174
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ELMO domain-containing protein A
Location	Carg_Chr20: 2736730 .. 2739319 (+)
RNA-Seq Expression	Carg03174
Synteny	Carg03174

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTGTGGTAAATGTAGCTAATGCTATGGTTGGACCACGATCATGGATCGGAGGAATCTTCAATCGCTCTGGCAATAAAAAGAACGATAAGTTTCAATACCCTTTGAGTCCTCTTCAGGTAATTAACGTCAATAATGTGTTGGTAGTCTGTGTACTGTGTCTCATTCATTGGCTTTATTTGTGTTGCATTATTCCACTAGTGGTATGGTTTGTAAGTGTGAGGAAAGTTTTACCTCTTGTTCATGAAAAGGCATTGGCACCAATAAGTGGGACTATAATATTGTCTGTTTTGGCCCGTTACGTATCGCCGTCAGCCTCGCGGTTTTAAAACGCGTCTACTAGTGAGAGGTTTCTACACCTTTATAAGGAAGGTTTCCACACCCTTATAAGGAAGGTTTCCACACCCTTATAAGGACTGTTTCGTTTCTGACTAAAGAAGATTTTGAGCTTTGCACATCCCCTTTCTCTCCACCTACCTATAGAGCATGCATGTAATAGAACTAGAACCTTGTGTTTCTATCAACATAACTTTCTCCAACAGGTTGTAAGTTTATATCTCCACCCCTAACATCTGATGTAATGTTCTTAAAACAATATATTCTCATGTGATTGCGAGAATGTCTATATGCATGGATTATGGAGCCTTATATGCCCTCAGTTTATTAATTTAGAACTCGTCGTCCACTTTTCATTGGATGAGACGTTCTAATGTTCAAGTAAACATACTTATATTTAAGACTGTTTTTCTAATGGCTTGTTGATAGGGGTGTATGAAGGTTGAGCATGTATGTTCTAACATGTGACTTATTCTACCAGGAGGAAAGGCTTCAGAGGCTACAAGACCGAATTCATATACCTTATGATGAGATGTGCATTGAGCATCAAGTACTTTTCTTTATTCGTTTTAGCACTCTTCAGGTTTTAAACTTCTATGCTCTATGAAGATTATCTGAGCGCTCTGAGATGAGTCTAAGCAACGAACTCACTGCATTTTTCGTCATTGACAAGATCCCATTTTAGAAGCCCAAAACCTCGTCCTTGATTTGAGTTTTGATCTATTTGAATTTTGAATAAGGCCATCATTCATGTCTTTCATTTCTACTGCATGCAGACAGCTAGAAGTTTAGCGTTTTCCCATTTATGCTTTGTTTTCATCATCTGTGAGCTCAAGTAATCATCCTTCATTATTTATAGGAAGCTCTGAGGGCATTGTGGGATGTTGCCTTTCCAGATGTTGAGCTTAAAGGGTTGATATCTGAGCAATGGAAAGAAATGGGTTGGCAAGGTGCTAACCCATCCACTGACTTTAGGTATAATCCAAGCCACCATTTCAATGTATATTCTTTGTTGTCTTACCTCTTATTAATGGTGATAGTGAAGTAAATGTTGAGGCTTGTTTTAATTTTTACAAAGACTTAGCAACCCATCTAAAATATAGTTTGAAGACTAGAGCTATCATAGGAGACGAGCAACATTATTTGTAACAGCCCAAGCTCACTGCTAACAAATATTATCCTTTTGGGCTTTCCTTTCAAGGTTTTTAGAACGCGTCGTCTAGGAAGAAGTTTCCACACCCTTATAAAGAATGTTTTGTTCCCCTCTCCAATTGATGTGCGATCTCACAATCCAACTGTAACGCCCCTAATTTTCTTAGCCTAAATTAGTGGCGTTACAAATTGTATTAGAGCCAGACACTGAGCAGTGTGCTAGCAAGGACGCTGGGTCCTGAAGGGGGTGGATTGCGAGATCCCACATTGGTTGGAGAGGGGAACAAAGCTTTTTTTGTAAGGGTGTGGAAACCTTTATCTAGCAGACGCGTTTTAAAAACCTTGTGGCGAAAGCCCAAAGAGGACAATATCTGCTAGTGGTGGGTTTAGAGAGATTGAGCGTGTGAGACAGTACAGTGCACACACGTGCGTACATCTGCGTTTGATTGAGGGAGGAATTCATTTTCTTCTAAATGCATTACACTTTAGTGGATTGGTAAAAAGAAACTGTTCATATGTTAGCTGAATACTTTGTTTTTTATACTTAAAATCAAATATTTTCTCACAGTTGGCTGCTCTTCTTAGGGGTTGCGGTTTCATTTCCCTTGAAAACTTGCTGTTTTTTTCCAGGACATTTCCGGTATGTCCTATTCTGTTGTATCCTCGTTTCCTTAATGCGTGAGTGAGTTATCACTAATCTCATACAAATTCAAAATGAAGGCATCTTTTCGTAGGTTATTATGGAAGGAAGGTGGCAATCGAGCAACTTGGGAATATCCGTTTGCTGTTGCTGGCATAAACATATCGTATATGTTGATTCAAATGTTGGATTTAAAATCAGGTATGTATCTGCATTTTTCGACTCGAATAGTTCGCTTTAATAATGGGAATGATCTCTGCAGCAGGTCTTGTTACCATGAACCCAACAAAATATGTAACAAAACCAATAACAAAGAACATGATTTAAAAGCCTTCTCGAAATCAACTCTGTCTAATACGTTCCCATCCCAGCAACTAAAAATTTTTGTTATCTCATCTCTTGTGTACAGCAAAGCCAAAGTGTATTCCAGGACTCAATTTTATGAGACTATTAGGAGGTACTGA

mRNA sequence

CTTGTGGTAAATGTAGCTAATGCTATGGTTGGACCACGATCATGGATCGGAGGAATCTTCAATCGCTCTGGCAATAAAAAGAACGATAAGTTTCAATACCCTTTGAGTCCTCTTCAGGAGGAAAGGCTTCAGAGGCTACAAGACCGAATTCATATACCTTATGATGAGATGTGCATTGAGCATCAAGTACTTTTCTTTATTCGTTTTAGCACTCTTCAGGAAGCTCTGAGGGCATTGTGGGATGTTGCCTTTCCAGATGTTGAGCTTAAAGGGTTGATATCTGAGCAATGGAAAGAAATGGGTTGGCAAGGTGCTAACCCATCCACTGACTTTAGGGGTTGCGGTTTCATTTCCCTTGAAAACTTGCTGTTTTTTTCCAGGACATTTCCGGCATCTTTTCGTAGGTTATTATGGAAGGAAGGTGGCAATCGAGCAACTTGGGAATATCCGTTTGCTGTTGCTGGCATAAACATATCGTATATGTTGATTCAAATGTTGGATTTAAAATCAGGTATGTATCTGCATTTTTCGACTCGAATAGTTCGCTTTAATAATGGGAATGATCTCTGCAGCAGGTCTTGTTACCATGAACCCAACAAAATATGTAACAAAACCAATAACAAAGAACATGATTTAAAAGCCTTCTCGAAATCAACTCTGTCTAATACGTTCCCATCCCAGCAACTAAAAATTTTTGTTATCTCATCTCTTGTGTACAGCAAAGCCAAAGTGTATTCCAGGACTCAATTTTATGAGACTATTAGGAGGTACTGA

Coding sequence (CDS)

CTTGTGGTAAATGTAGCTAATGCTATGGTTGGACCACGATCATGGATCGGAGGAATCTTCAATCGCTCTGGCAATAAAAAGAACGATAAGTTTCAATACCCTTTGAGTCCTCTTCAGGAGGAAAGGCTTCAGAGGCTACAAGACCGAATTCATATACCTTATGATGAGATGTGCATTGAGCATCAAGTACTTTTCTTTATTCGTTTTAGCACTCTTCAGGAAGCTCTGAGGGCATTGTGGGATGTTGCCTTTCCAGATGTTGAGCTTAAAGGGTTGATATCTGAGCAATGGAAAGAAATGGGTTGGCAAGGTGCTAACCCATCCACTGACTTTAGGGGTTGCGGTTTCATTTCCCTTGAAAACTTGCTGTTTTTTTCCAGGACATTTCCGGCATCTTTTCGTAGGTTATTATGGAAGGAAGGTGGCAATCGAGCAACTTGGGAATATCCGTTTGCTGTTGCTGGCATAAACATATCGTATATGTTGATTCAAATGTTGGATTTAAAATCAGGTATGTATCTGCATTTTTCGACTCGAATAGTTCGCTTTAATAATGGGAATGATCTCTGCAGCAGGTCTTGTTACCATGAACCCAACAAAATATGTAACAAAACCAATAACAAAGAACATGATTTAAAAGCCTTCTCGAAATCAACTCTGTCTAATACGTTCCCATCCCAGCAACTAAAAATTTTTGTTATCTCATCTCTTGTGTACAGCAAAGCCAAAGTGTATTCCAGGACTCAATTTTATGAGACTATTAGGAGGTACTGA

Protein sequence

LVVNVANAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKSGMYLHFSTRIVRFNNGNDLCSRSCYHEPNKICNKTNNKEHDLKAFSKSTLSNTFPSQQLKIFVISSLVYSKAKVYSRTQFYETIRRY

Homology

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BLAST of Carg03174 vs. NCBI nr
Match: KAG7010691.1 (ELMO domain-containing protein B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 529.3 bits (1362), Expect = 1.9e-146
Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 0


Query: 1   LVVNVANAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIE 60
           LVVNVANAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIE
Sbjct: 1   LVVNVANAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIE 60

Query: 61  HQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLE 120
           HQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLE
Sbjct: 61  HQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLE 120

Query: 121 NLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKSGMYLHFSTRI 180
           NLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKSGMYLHFSTRI
Sbjct: 121 NLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKSGMYLHFSTRI 180

Query: 181 VRFNNGNDLCSRSCYHEPNKICNKTNNKEHDLKAFSKSTLSNTFPSQQLKIFVISSLVYS 240
           VRFNNGNDLCSRSCYHEPNKICNKTNNKEHDLKAFSKSTLSNTFPSQQLKIFVISSLVYS
Sbjct: 181 VRFNNGNDLCSRSCYHEPNKICNKTNNKEHDLKAFSKSTLSNTFPSQQLKIFVISSLVYS 240

Query: 241 KAKVYSRTQFYETIRRY 258
           KAKVYSRTQFYETIRRY
Sbjct: 241 KAKVYSRTQFYETIRRY 257

[Close]

BLAST of Carg03174 vs. NCBI nr
Match: XP_022943361.1 (ELMO domain-containing protein A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 318.5 bits (815), Expect = 5.1e-83
Identity = 153/164 (93.29%), Postives = 153/164 (93.29%), Query Frame = 0


Query: 7   NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFF 66
           NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH     
Sbjct: 54  NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH----- 113

Query: 67  IRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 126
                 QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS
Sbjct: 114 ------QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 173

Query: 127 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 174 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 206

[Close]

BLAST of Carg03174 vs. NCBI nr
Match: KAG6570845.1 (ELMO domain-containing protein A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.5 bits (815), Expect = 5.1e-83
Identity = 153/164 (93.29%), Postives = 153/164 (93.29%), Query Frame = 0


Query: 7   NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFF 66
           NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH     
Sbjct: 61  NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH----- 120

Query: 67  IRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 126
                 QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS
Sbjct: 121 ------QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 180

Query: 127 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 181 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 213

[Close]

BLAST of Carg03174 vs. NCBI nr
Match: XP_022943363.1 (ELMO domain-containing protein A-like isoform X3 [Cucurbita moschata] >XP_022943364.1 ELMO domain-containing protein A-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 314.7 bits (805), Expect = 7.4e-82
Identity = 151/162 (93.21%), Postives = 151/162 (93.21%), Query Frame = 0


Query: 9   MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFFIR 68
           MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH       
Sbjct: 1   MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH------- 60

Query: 69  FSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT 128
               QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT
Sbjct: 61  ----QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT 120

Query: 129 FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 121 FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 151

[Close]

BLAST of Carg03174 vs. NCBI nr
Match: XP_023513039.1 (ELMO domain-containing protein A-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.8 bits (795), Expect = 1.1e-80
Identity = 151/164 (92.07%), Postives = 151/164 (92.07%), Query Frame = 0


Query: 7   NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFF 66
           NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSP QEERLQRLQDRIHIPYDEMCIEH     
Sbjct: 54  NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPRQEERLQRLQDRIHIPYDEMCIEH----- 113

Query: 67  IRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 126
                 QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS
Sbjct: 114 ------QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 173

Query: 127 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           RTFPASFRRLL KEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 174 RTFPASFRRLLSKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 206

[Close]

BLAST of Carg03174 vs. ExPASy Swiss-Prot
Match: Q54VR8 (ELMO domain-containing protein B OS=Dictyostelium discoideum OX=44689 GN=elmoB PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.1e-17
Identity = 48/144 (33.33%), Postives = 78/144 (54.17%), Query Frame = 0


Query: 46  LQDRIHIPYDEMCIEHQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGA 105
           ++ +  IPYD   +EH+            +L  LW+   PDV     +S++W  +G+QG 
Sbjct: 110 IEKKQKIPYDNNNLEHEA-----------SLERLWEALLPDVRRSARLSKEWGTLGFQGM 169

Query: 106 NPSTDFRGCGFISLENLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQM 165
           +P+TDFRG G + L+NL++FS       R +L K   ++    YPFA+ GINI+ +++ +
Sbjct: 170 DPATDFRGMGILGLDNLIYFSTQHSEDAREIL-KNSNSKCC--YPFAITGINITALVLNL 229

Query: 166 LDLKSGMYLHFSTRIVRFNNGNDL 190
           +D       HF  +I  F NG+ L
Sbjct: 230 IDKP-----HF--KIYFFKNGSTL 232

[Close]

BLAST of Carg03174 vs. ExPASy Swiss-Prot
Match: Q54YW1 (ELMO domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=elmoA PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.6e-16
Identity = 43/124 (34.68%), Postives = 71/124 (57.26%), Query Frame = 0


Query: 39  QEERLQRLQDRIHIPYDEMCIEHQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWK 98
           Q  R Q + +R ++ +++   EH  L           L  LW + +P V+L+  +SEQWK
Sbjct: 362 QRHRFQVITNRKNVTFNKESSEHDAL-----------LMKLWSLTYPGVKLESRVSEQWK 421

Query: 99  EMGWQGANPSTDFRGCGFISLENLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINI 158
           +MG+QG +P TDFR  G   L+NL++F++ +   FR+++     +R   EYP A AGI +
Sbjct: 422 QMGFQGTDPCTDFRAMGIWGLDNLIYFAQNYNEKFRKIV-NSQIDRKEREYPTATAGIVL 473

Query: 159 SYML 163
           ++ L
Sbjct: 482 TFEL 473

[Close]

BLAST of Carg03174 vs. ExPASy Swiss-Prot
Match: Q8IZ81 (ELMO domain-containing protein 2 OS=Homo sapiens OX=9606 GN=ELMOD2 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.9e-14
Identity = 39/114 (34.21%), Postives = 62/114 (54.39%), Query Frame = 0


Query: 53  PYDEMCIEHQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFR 112
           PYD   ++H           +E L  LW++  P  +L   IS+QW E+G+QG +P TDFR
Sbjct: 119 PYDSDNLQH-----------EELLMKLWNLLMPTKKLNARISKQWAEIGFQGDDPKTDFR 178

Query: 113 GCGFISLENLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQML 167
           G G + L NL++FS  + +   ++L +   N     Y +A+ GIN++ M   +L
Sbjct: 179 GMGILGLINLVYFSENYTSEAHQILSR--SNHPKLGYSYAIVGINLTEMAYSLL 219

[Close]

BLAST of Carg03174 vs. ExPASy Swiss-Prot
Match: Q08DZ3 (ELMO domain-containing protein 2 OS=Bos taurus OX=9913 GN=ELMOD2 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-13
Identity = 39/114 (34.21%), Postives = 63/114 (55.26%), Query Frame = 0


Query: 53  PYDEMCIEHQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFR 112
           PYD   ++H+ L           L  LW++  P  +LK  IS+QW ++G+QG +P TDFR
Sbjct: 119 PYDSDNLQHEKL-----------LIKLWNLLMPTKKLKARISKQWADIGFQGDDPKTDFR 178

Query: 113 GCGFISLENLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQML 167
           G G + L NL++FS  + +   ++L +   N     Y +A+ GIN++ M   +L
Sbjct: 179 GMGILGLINLVYFSENYTSEAHQILSR--SNHPKLGYSYAIVGINLTEMAYSLL 219

[Close]

BLAST of Carg03174 vs. ExPASy Swiss-Prot
Match: Q8BGF6 (ELMO domain-containing protein 2 OS=Mus musculus OX=10090 GN=Elmod2 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.3e-12
Identity = 37/114 (32.46%), Postives = 60/114 (52.63%), Query Frame = 0


Query: 53  PYDEMCIEHQVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFR 112
           PYD    +H           ++ L  LW +  P  +L   IS+QW ++G+QG +P TDFR
Sbjct: 119 PYDSANAQH-----------EKMLLKLWSLLMPTKKLTARISKQWADIGFQGDDPKTDFR 178

Query: 113 GCGFISLENLLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQML 167
           G G + L NL++FS  + +   ++L +   N     Y +A+ GIN++ M   +L
Sbjct: 179 GMGILGLINLVYFSENYTSEAHQILSR--SNHPKLGYSYAIVGINLTEMAYSLL 219

[Close]

BLAST of Carg03174 vs. ExPASy TrEMBL
Match: A0A6J1FRI0 (ELMO domain-containing protein A-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448148 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 2.5e-83
Identity = 153/164 (93.29%), Postives = 153/164 (93.29%), Query Frame = 0


Query: 7   NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFF 66
           NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH     
Sbjct: 54  NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH----- 113

Query: 67  IRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 126
                 QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS
Sbjct: 114 ------QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 173

Query: 127 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 174 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 206

[Close]

BLAST of Carg03174 vs. ExPASy TrEMBL
Match: A0A6J1FU36 (ELMO domain-containing protein A-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111448148 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 3.6e-82
Identity = 151/162 (93.21%), Postives = 151/162 (93.21%), Query Frame = 0


Query: 9   MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFFIR 68
           MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH       
Sbjct: 1   MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH------- 60

Query: 69  FSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT 128
               QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT
Sbjct: 61  ----QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT 120

Query: 129 FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 121 FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 151

[Close]

BLAST of Carg03174 vs. ExPASy TrEMBL
Match: A0A6J1J7B4 (ELMO domain-containing protein A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484122 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.2e-80
Identity = 151/164 (92.07%), Postives = 151/164 (92.07%), Query Frame = 0


Query: 7   NAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFF 66
           NAMVGPRSWI GIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH     
Sbjct: 54  NAMVGPRSWIVGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH----- 113

Query: 67  IRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 126
                 QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS
Sbjct: 114 ------QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFS 173

Query: 127 RTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           RTFPASFRRLL KEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 174 RTFPASFRRLLSKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 206

[Close]

BLAST of Carg03174 vs. ExPASy TrEMBL
Match: A0A6J1JFV1 (ELMO domain-containing protein A-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111484122 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.7e-79
Identity = 149/162 (91.98%), Postives = 149/162 (91.98%), Query Frame = 0


Query: 9   MVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQVLFFIR 68
           MVGPRSWI GIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH       
Sbjct: 1   MVGPRSWIVGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH------- 60

Query: 69  FSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT 128
               QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT
Sbjct: 61  ----QEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENLLFFSRT 120

Query: 129 FPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           FPASFRRLL KEGGNRATWEYPFAVAGINISYMLIQMLDLKS
Sbjct: 121 FPASFRRLLSKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 151

[Close]

BLAST of Carg03174 vs. ExPASy TrEMBL
Match: A0A6J1CGE4 (ELMO domain-containing protein A isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010989 PE=4 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 3.6e-74
Identity = 138/169 (81.66%), Postives = 147/169 (86.98%), Query Frame = 0


Query: 2   VVNVANAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH 61
           VVNVANAMVGPRSWI G+FNRSGNKKND+FQYPLSPLQEERLQRLQ+R+ IP+DE CI H
Sbjct: 45  VVNVANAMVGPRSWITGLFNRSGNKKNDRFQYPLSPLQEERLQRLQERMQIPFDETCIVH 104

Query: 62  QVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLEN 121
                      QEALR LW+ AFPDVELKG+ISEQWKEMGWQG NPSTDFRGCGFISLEN
Sbjct: 105 -----------QEALRTLWNAAFPDVELKGMISEQWKEMGWQGPNPSTDFRGCGFISLEN 164

Query: 122 LLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           LLFFSRTFPASFRRLL KE GNRA WEYPFAVAGIN+S+MLIQMLDL S
Sbjct: 165 LLFFSRTFPASFRRLLLKEDGNRAEWEYPFAVAGINVSFMLIQMLDLNS 202

[Close]

BLAST of Carg03174 vs. TAIR 10
Match: AT1G03620.1 (ELMO/CED-12 family protein )

HSP 1 Score: 228.8 bits (582), Expect = 5.0e-60
Identity = 109/168 (64.88%), Postives = 131/168 (77.98%), Query Frame = 0


Query: 3   VNVANAMVGPRSWIGGIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQ 62
           V  A  MVG RSWIGG+F RS  +++    Y LSPLQEERLQRLQDR+ +P+DE   +H 
Sbjct: 27  VVTATGMVGTRSWIGGLFTRSNRRQDKAVDYTLSPLQEERLQRLQDRMVVPFDETRPDH- 86

Query: 63  VLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENL 122
                     QE+L+ALW+VAFP+V L GL++EQWKEMGWQG NPSTDFRGCGFI+LENL
Sbjct: 87  ----------QESLKALWNVAFPNVHLTGLVTEQWKEMGWQGPNPSTDFRGCGFIALENL 146

Query: 123 LFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           LF +RT+P  FRRLL K+ G+RA WEYPFAVAGINIS+MLIQMLDL++
Sbjct: 147 LFSARTYPVCFRRLLLKQRGDRAKWEYPFAVAGINISFMLIQMLDLQN 183

[Close]

BLAST of Carg03174 vs. TAIR 10
Match: AT3G60260.1 (ELMO/CED-12 family protein )

HSP 1 Score: 178.7 bits (452), Expect = 5.9e-45
Identity = 89/168 (52.98%), Postives = 114/168 (67.86%), Query Frame = 0


Query: 6   ANAMVGPRSWIG---GIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQ 65
           A  + G  +W+G         G   + +  + L+P QEE LQRLQ RI + YD    +H 
Sbjct: 29  AEVVAGSAAWLGRGLSCVCVQGRDGDPRPSFDLTPAQEECLQRLQSRIDVAYDSSIPQH- 88

Query: 66  VLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENL 125
                     QEAL+ LW +AFP+ EL G++S+QWKEMGWQG +PSTDFRG GFISLENL
Sbjct: 89  ----------QEALKDLWKLAFPEEELHGIVSDQWKEMGWQGKDPSTDFRGGGFISLENL 148

Query: 126 LFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           L+F+R FP SF  LL K+ G+R+ WEYPFAVAGINI++MLIQMLDL++
Sbjct: 149 LYFARKFPKSFHGLLRKQVGDRSVWEYPFAVAGINITFMLIQMLDLEA 185

[Close]

BLAST of Carg03174 vs. TAIR 10
Match: AT3G60260.2 (ELMO/CED-12 family protein )

HSP 1 Score: 178.7 bits (452), Expect = 5.9e-45
Identity = 89/168 (52.98%), Postives = 114/168 (67.86%), Query Frame = 0


Query: 6   ANAMVGPRSWIG---GIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQ 65
           A  + G  +W+G         G   + +  + L+P QEE LQRLQ RI + YD    +H 
Sbjct: 29  AEVVAGSAAWLGRGLSCVCVQGRDGDPRPSFDLTPAQEECLQRLQSRIDVAYDSSIPQH- 88

Query: 66  VLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENL 125
                     QEAL+ LW +AFP+ EL G++S+QWKEMGWQG +PSTDFRG GFISLENL
Sbjct: 89  ----------QEALKDLWKLAFPEEELHGIVSDQWKEMGWQGKDPSTDFRGGGFISLENL 148

Query: 126 LFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           L+F+R FP SF  LL K+ G+R+ WEYPFAVAGINI++MLIQMLDL++
Sbjct: 149 LYFARKFPKSFHGLLRKQVGDRSVWEYPFAVAGINITFMLIQMLDLEA 185

[Close]

BLAST of Carg03174 vs. TAIR 10
Match: AT3G60260.4 (ELMO/CED-12 family protein )

HSP 1 Score: 178.7 bits (452), Expect = 5.9e-45
Identity = 89/168 (52.98%), Postives = 114/168 (67.86%), Query Frame = 0


Query: 6   ANAMVGPRSWIG---GIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEHQ 65
           A  + G  +W+G         G   + +  + L+P QEE LQRLQ RI + YD    +H 
Sbjct: 29  AEVVAGSAAWLGRGLSCVCVQGRDGDPRPSFDLTPAQEECLQRLQSRIDVAYDSSIPQH- 88

Query: 66  VLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLENL 125
                     QEAL+ LW +AFP+ EL G++S+QWKEMGWQG +PSTDFRG GFISLENL
Sbjct: 89  ----------QEALKDLWKLAFPEEELHGIVSDQWKEMGWQGKDPSTDFRGGGFISLENL 148

Query: 126 LFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           L+F+R FP SF  LL K+ G+R+ WEYPFAVAGINI++MLIQMLDL++
Sbjct: 149 LYFARKFPKSFHGLLRKQVGDRSVWEYPFAVAGINITFMLIQMLDLEA 185

[Close]

BLAST of Carg03174 vs. TAIR 10
Match: AT3G60260.3 (ELMO/CED-12 family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.0e-44
Identity = 88/169 (52.07%), Postives = 115/169 (68.05%), Query Frame = 0


Query: 5   VANAMVGPRSWIG---GIFNRSGNKKNDKFQYPLSPLQEERLQRLQDRIHIPYDEMCIEH 64
           ++  + G  +W+G         G   + +  + L+P QEE LQRLQ RI + YD    +H
Sbjct: 6   LSEVVAGSAAWLGRGLSCVCVQGRDGDPRPSFDLTPAQEECLQRLQSRIDVAYDSSIPQH 65

Query: 65  QVLFFIRFSTLQEALRALWDVAFPDVELKGLISEQWKEMGWQGANPSTDFRGCGFISLEN 124
                      QEAL+ LW +AFP+ EL G++S+QWKEMGWQG +PSTDFRG GFISLEN
Sbjct: 66  -----------QEALKDLWKLAFPEEELHGIVSDQWKEMGWQGKDPSTDFRGGGFISLEN 125

Query: 125 LLFFSRTFPASFRRLLWKEGGNRATWEYPFAVAGINISYMLIQMLDLKS 171
           LL+F+R FP SF  LL K+ G+R+ WEYPFAVAGINI++MLIQMLDL++
Sbjct: 126 LLYFARKFPKSFHGLLRKQVGDRSVWEYPFAVAGINITFMLIQMLDLEA 163

The following BLAST results are available for this feature:

BLAST of Carg03174 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010691.1	1.9e-146	100.00	ELMO domain-containing protein B, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022943361.1	5.1e-83	93.29	ELMO domain-containing protein A-like isoform X1 [Cucurbita moschata]	[more]
KAG6570845.1	5.1e-83	93.29	ELMO domain-containing protein A, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022943363.1	7.4e-82	93.21	ELMO domain-containing protein A-like isoform X3 [Cucurbita moschata] >XP_022943...	[more]
XP_023513039.1	1.1e-80	92.07	ELMO domain-containing protein A-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg03174 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54VR8	5.1e-17	33.33	ELMO domain-containing protein B OS=Dictyostelium discoideum OX=44689 GN=elmoB P...	[more]
Q54YW1	5.6e-16	34.68	ELMO domain-containing protein A OS=Dictyostelium discoideum OX=44689 GN=elmoA P...	[more]
Q8IZ81	6.9e-14	34.21	ELMO domain-containing protein 2 OS=Homo sapiens OX=9606 GN=ELMOD2 PE=1 SV=1	[more]
Q08DZ3	1.5e-13	34.21	ELMO domain-containing protein 2 OS=Bos taurus OX=9913 GN=ELMOD2 PE=1 SV=1	[more]
Q8BGF6	1.3e-12	32.46	ELMO domain-containing protein 2 OS=Mus musculus OX=10090 GN=Elmod2 PE=1 SV=1	[more]

BLAST of Carg03174 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FRI0	2.5e-83	93.29	ELMO domain-containing protein A-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1FU36	3.6e-82	93.21	ELMO domain-containing protein A-like isoform X3 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1J7B4	1.2e-80	92.07	ELMO domain-containing protein A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1JFV1	1.7e-79	91.98	ELMO domain-containing protein A-like isoform X3 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1CGE4	3.6e-74	81.66	ELMO domain-containing protein A isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of Carg03174 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G03620.1	5.0e-60	64.88	ELMO/CED-12 family protein	[more]
AT3G60260.1	5.9e-45	52.98	ELMO/CED-12 family protein	[more]
AT3G60260.2	5.9e-45	52.98	ELMO/CED-12 family protein	[more]
AT3G60260.4	5.9e-45	52.98	ELMO/CED-12 family protein	[more]
AT3G60260.3	1.0e-44	52.07	ELMO/CED-12 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006816	ELMO domain	PFAM	PF04727	ELMO_CED12	coord: 64..169 e-value: 2.4E-30 score: 105.7
IPR006816	ELMO domain	PROSITE	PS51335	ELMO	coord: 71..257 score: 18.784479
None	No IPR available	PANTHER	PTHR12771:SF64	BNAC05G01970D PROTEIN	coord: 3..170
None	No IPR available	PANTHER	PTHR12771	ENGULFMENT AND CELL MOTILITY	coord: 3..170

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03174-RA	Carg03174-RA	mRNA