Homology
BLAST of Carg02987 vs. NCBI nr
Match:
KAG7024560.1 (Isoamylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1549.6 bits (4011), Expect = 0.0e+00
Identity = 738/738 (100.00%), Postives = 738/738 (100.00%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV
Sbjct: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG
Sbjct: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA
Sbjct: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN
Sbjct: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI
Sbjct: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 738
BLAST of Carg02987 vs. NCBI nr
Match:
XP_022936809.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata])
HSP 1 Score: 1445.3 bits (3740), Expect = 0.0e+00
Identity = 696/738 (94.31%), Postives = 696/738 (94.31%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV
Sbjct: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG
Sbjct: 181 -------------------DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA
Sbjct: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN
Sbjct: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI
Sbjct: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 696
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 696
BLAST of Carg02987 vs. NCBI nr
Match:
XP_023535183.1 (isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 690/738 (93.50%), Postives = 692/738 (93.77%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPG SSSSQEVFSE KQGGV
Sbjct: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPG--SSSSQEVFSEAKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
KICTVSAFGRRDQDRVLEQEAPENLDTGPMLK+VPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKIVPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG
Sbjct: 181 -------------------DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA
Sbjct: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RGPDGAPLSAPPLIRAISKD ILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGPDGAPLSAPPLIRAISKDDILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKP+HGINFVIAHDGFTLRDLVSYNVKHNN
Sbjct: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPFHGINFVIAHDGFTLRDLVSYNVKHNN 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI
Sbjct: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 694
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 694
BLAST of Carg02987 vs. NCBI nr
Match:
XP_022975928.1 (isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima])
HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 688/738 (93.22%), Postives = 691/738 (93.63%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKR PG SSSSQEVFSETKQGGV
Sbjct: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRGPG--SSSSQEVFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
KICTVSAFGRRDQD VLEQEAPENLDTGPMLK+VPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KICTVSAFGRRDQDHVLEQEAPENLDTGPMLKIVPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DLPRKNVLYGYRIDGPQGWHHGHRFDD IVLLDPYAKFVEG
Sbjct: 181 -------------------DLPRKNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
S+RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA
Sbjct: 301 SLRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN
Sbjct: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFH+ALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHMALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI
Sbjct: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 694
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 694
BLAST of Carg02987 vs. NCBI nr
Match:
XP_038896292.1 (isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoamylase 3, chloroplastic isoform X1 [Benincasa hispida])
HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 642/738 (86.99%), Postives = 666/738 (90.24%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
M+R +PLHHSA LQLPLFASSCT AA+SV+FR KPQGK APG SSS SQ++FSE KQGGV
Sbjct: 1 MVRYLPLHHSASLQLPLFASSCTRAAYSVEFRSKPQGKLAPG-SSSCSQDIFSEIKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
K+CTV AFGRRDQ+RVLEQEAPENLDTGPMLK+ PGQAFPLGVSEV +GINFAIFSQHAT
Sbjct: 61 KLCTVCAFGRRDQERVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVADGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
+VTICLS NERIDDR LEFKLDPD+NRTGDIWHIC Q
Sbjct: 121 SVTICLSLNERIDDRMLEFKLDPDNNRTGDIWHICFQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DLPRK+VLYGYRIDGPQGWHHGHRFD S VLLDPYAKFVEG
Sbjct: 181 -------------------DLPRKSVLYGYRIDGPQGWHHGHRFDGSTVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWG+DYKLPNILEKDLVIYEMNVR FTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGNDYKLPNILEKDLVIYEMNVRGFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
S RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR+HMIN WGYSTINFFA
Sbjct: 301 STRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINMWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALH AGIEVILDVVYNHTNEADDV PYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHAAGIEVILDVVYNHTNEADDVNPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQ+LNFSGCGNTLNCNHPVVMELILDSLRHWVV+YHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQYLNFSGCGNTLNCNHPVVMELILDSLRHWVVDYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RG DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRV+GSSDLYKVN RKPYHGINFVIAHDGFTLRDLVSYNVKHN+
Sbjct: 541 IRKFIKGDCGMKGSFATRVAGSSDLYKVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHND 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
NGEGGNDG NDNFSWNCGFEGETED +I+ALR+RQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 VNGEGGNDGSNDNFSWNCGFEGETEDTSIKALRTRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHD ALNHFLW QLEARK DHFRFFSEVIKFRRKHPHLFSRENFL +NDI
Sbjct: 661 HTRYGNNNSYGHDNALNHFLWDQLEARKTDHFRFFSEVIKFRRKHPHLFSRENFLSKNDI 695
Query: 721 TWHESNWDNPESKFLAYT 739
+WHESNWDNPESKFLAYT
Sbjct: 721 SWHESNWDNPESKFLAYT 695
BLAST of Carg02987 vs. ExPASy Swiss-Prot
Match:
Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)
HSP 1 Score: 1014.2 bits (2621), Expect = 7.4e-295
Identity = 487/681 (71.51%), Postives = 544/681 (79.88%), Query Frame = 0
Query: 64 TVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHATAVT 123
T+ RR +RV+E+EA +T + KV G+ PLGVS+VD GINFA+FSQ+AT+VT
Sbjct: 57 TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116
Query: 124 ICLSF-----NERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSS 183
+CLS ++ DD +E LDP N+TGD WHIC++
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVE--------------------- 176
Query: 184 LILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFV 243
DLP NVLYGYR+DGP W GHRFD SI+LLDPYAK V
Sbjct: 177 ---------------------DLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLV 236
Query: 244 EGRRYFG-GENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSG 303
+G FG K + F GTYDF+S PFDWGDDYK PNI EKDLVIYEMNVRAFTADESSG
Sbjct: 237 KGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSG 296
Query: 304 LASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTIN 363
+ +I GSYLG IEKIPHL +LG+NAVELLPVFEFDELE QR NPR+HM+NTWGYST+N
Sbjct: 297 MDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVN 356
Query: 364 FFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRG 423
FFAPMSRYAS G P+ AS+EFKEMVKALH AGIEVILDVVYNHTNEADD YPYTTSFRG
Sbjct: 357 FFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRG 416
Query: 424 IDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLAS 483
IDNKVYYMLD N Q LNFSGCGNTLNCNHPVVMELILDSLRHWV EYHVDGFRFDLAS
Sbjct: 417 IDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLAS 476
Query: 484 VLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIY 543
VLCR DG+PLSAPPLIRAI+KD++LSRCKIIAEPWDCGGLYLVG+FPNWDRWAEWNG+Y
Sbjct: 477 VLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMY 536
Query: 544 RDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVK 603
RDD+R+FIKGD GMKGSFATRVSGSSDLY+VN RKPYHG+NFVIAHDGFTLRDLVSYN K
Sbjct: 537 RDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFK 596
Query: 604 HNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGD 663
HN ANGEGGNDGCNDN SWNCGFEGET DA+I++LR+RQMKNFHLALM SQGTPMMLMGD
Sbjct: 597 HNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGD 656
Query: 664 EYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGE 723
EYGHTRYGNNNSYGHDT+LN+F W +L+A+K++HFRFFSEVIKFR H H+ ENFL +
Sbjct: 657 EYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSH-HVLKHENFLTQ 691
Query: 724 NDITWHESNWDNPESKFLAYT 739
+ITWHE NWDN ESKFLA+T
Sbjct: 717 GEITWHEDNWDNSESKFLAFT 691
BLAST of Carg02987 vs. ExPASy Swiss-Prot
Match:
B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)
HSP 1 Score: 992.3 bits (2564), Expect = 3.0e-288
Identity = 471/659 (71.47%), Postives = 528/659 (80.12%), Query Frame = 0
Query: 86 DTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHATAVTICLSFNER--IDDR---TLEFK 145
DT K G+AFPLGVS+V+ G+NFA+FSQHA++V +CL R D++ +EF
Sbjct: 93 DTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQHASSVILCLKLPGRGTEDEKGADVVEFV 152
Query: 146 LDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLILAVTYKLYGTVLWPLDLKMD 205
LD N+TGDIWH+ ++
Sbjct: 153 LDQQKNKTGDIWHVIVE------------------------------------------G 212
Query: 206 LPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEGRRYFG-GENKSSGFLGTYDF 265
LP VLYGYR+ GPQGW GHRFD S VLLDPYAK V GR+YFG E KSS GTYDF
Sbjct: 213 LPASGVLYGYRVGGPQGWDQGHRFDSSTVLLDPYAKLVSGRKYFGVAEEKSSQHFGTYDF 272
Query: 266 DSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLEL 325
DS PFDWGDDY+LPN+ E DLVIYEMNVRAFTADESSGL S+ RGSYLG+I+KIPHLLEL
Sbjct: 273 DSSPFDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLEL 332
Query: 326 GVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFAPMSRYASAGGGPLNASREF 385
GVNAVELLPVFE+DELEF+R+PNPR+HM+NTWGYSTINFFAPMSRYASAGGGP+ AS+E
Sbjct: 333 GVNAVELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAPMSRYASAGGGPVAASKEL 392
Query: 386 KEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSG 445
K+MVK LH AGIEVILDVVYNHTNEADD +PY TSFRGIDNKVYYMLDL N + LNFSG
Sbjct: 393 KQMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSG 452
Query: 446 CGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISK 505
CGNTLNCNHPVV ELILDSLRHWV EYH+DGFRFDLASVLCRGPDG PL APPLI+ I+K
Sbjct: 453 CGNTLNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCRGPDGCPLDAPPLIKEIAK 512
Query: 506 DAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRV 565
DA+LSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNG YRDD+R+FIKGD GMKG FATRV
Sbjct: 513 DAVLSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRV 572
Query: 566 SGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCG 625
SGS+DLY+VN RKPYHG+NFVIAHDGFTL DLVSYN+KHN+ANGEGG DGCNDNFSWNCG
Sbjct: 573 SGSADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCG 632
Query: 626 FEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHF 685
EGET D N+ +LRSRQMKNFH+ALM SQGTPMMLMGDEYGHTRYGNNNSYGHDT +N+F
Sbjct: 633 VEGETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNF 692
Query: 686 LWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHESNWDNPESKFLAYT 739
W QLE R+ HFRFFSE+IKFR +P + R+ FL +ND+TWHE W+N ESKFLA+T
Sbjct: 693 QWEQLEQRRDGHFRFFSEMIKFRHSNP-ILRRDRFLNKNDVTWHEDCWENQESKFLAFT 708
BLAST of Carg02987 vs. ExPASy Swiss-Prot
Match:
D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 550.4 bits (1417), Expect = 3.0e-155
Identity = 316/710 (44.51%), Postives = 403/710 (56.76%), Query Frame = 0
Query: 69 GRRDQDRVLEQE-------APENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHATA 128
G +++ V E+E PE G +V+ G PLG + +D G+NFA++S A+A
Sbjct: 65 GEDEEEGVEEEEEEVEAVVMPERYALGGACRVLAGMPAPLGATALDGGVNFAVYSAGASA 124
Query: 129 VTICLSFNERI--DDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSL 188
++CL + + D+ T E LDP NRTG++WH+ I+ L
Sbjct: 125 ASLCLFTPDDLEADEVTEEVPLDPLFNRTGNVWHVFIEGEL------------------- 184
Query: 189 ILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVE 248
N+LYGYR DG H G FD S V++DPYAK V
Sbjct: 185 ------------------------HNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVI 244
Query: 249 GRRYFG----GENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADES 308
R +G G + G FDW D L +KDLVIYEM++R FT S
Sbjct: 245 SRGEYGVPGPGGDCWPQMAGMIPLPYSTFDWQGDLPL-RYPQKDLVIYEMHLRGFTKHSS 304
Query: 309 SGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYST 368
S + G+Y+G I K+ +L ELGVN VEL+P EF+ELE+ + +N WGYST
Sbjct: 305 SNVEHP--GTYIGAISKLDYLKELGVNCVELMPCHEFNELEY----FSCSSKMNFWGYST 364
Query: 369 INFFAPMSRYASAGGGPLNASR----EFKEMVKALHGAGIEVILDVVYNHTNEADDVYPY 428
INFF+PM RY+S GG N R EFK V+ H GIEVI+DVV+NHT E ++ P
Sbjct: 365 INFFSPMIRYSS--GGIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGP- 424
Query: 429 TTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGF 488
SFRGIDN YYM L G+F N+SGCGNT NCNHPVV E I+D LR+WV E HVDGF
Sbjct: 425 ILSFRGIDNSTYYM--LAPKGEFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGF 484
Query: 489 RFDLASVLCRG-------------------PDGAPLSAPPLIRAISKDAILSRCKIIAEP 548
RFDLAS++ RG G PL+ PPLI IS D IL K+IAE
Sbjct: 485 RFDLASIMTRGCSLWDPVNVYGSPVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEA 544
Query: 549 WDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRK 608
WD GGLY VG+FP+W W+EWNG YRD +R+FIKG G G FA + GS LY+ RK
Sbjct: 545 WDAGGLYQVGQFPHWKIWSEWNGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRK 604
Query: 609 PYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDANIRAL 668
P+H INFV AHDGFTL DLV+YN K+N++NGE DG N N SWNCG EGE +++ L
Sbjct: 605 PWHSINFVCAHDGFTLADLVTYNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRL 664
Query: 669 RSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHF 728
R RQM+NF ++LM SQG PM MGDEYGHT+ GNNN+Y HD +N+F W + E D
Sbjct: 665 RKRQMRNFFVSLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKE-ESSDLQ 717
Query: 729 RFFSEVIKFRRKHPHLFSRENFLGENDITWHESNWDNPE----SKFLAYT 739
RF S + KFR++ L +F + WH P+ S+F+A++
Sbjct: 725 RFCSLMTKFRKQCESL-GLADFPTAQRLHWHGHQPGKPDWSETSRFVAFS 717
BLAST of Carg02987 vs. ExPASy Swiss-Prot
Match:
O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 546.6 bits (1407), Expect = 4.4e-154
Identity = 308/676 (45.56%), Postives = 394/676 (58.28%), Query Frame = 0
Query: 96 GQAFPLGVSEVDNGINFAIFSQHATAVTICL-SFNE-RIDDRTLEFKLDPDDNRTGDIWH 155
G P G + D+G+NF+++S ++ + TICL S ++ R + T E +LDP NRTG +WH
Sbjct: 72 GLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWH 131
Query: 156 ICIQFTLIILHSPFGSSVHRKPSSSLILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRID 215
+ L F K++LYGYR D
Sbjct: 132 -------VFLRGDF------------------------------------KDMLYGYRFD 191
Query: 216 GPQGWHHGHRFDDSIVLLDPYAKFVEGRRYFG----GENKSSGFLGTYDFDSLPFDWGDD 275
G GH +D S +LLDPYAK + R FG +N FDW D
Sbjct: 192 GKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGD 251
Query: 276 YKLPNILEKDLVIYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPV 335
L + +KDLVIYEM+VR FT ESS + G+Y GV EK+ HL ELG+N +EL+P
Sbjct: 252 MHL-KLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPC 311
Query: 336 FEFDELEFQRHPN-PRNHMINTWGYSTINFFAPMSRYASAGGGPL--NASREFKEMVKAL 395
EF+ELE+ + +H +N WGYSTI FF+PM RYASA A EFK +VK
Sbjct: 312 HEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEA 371
Query: 396 HGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNC 455
H GIEVI+DVV NHT E ++ P SFRG+DN VYYM L G+F N+SGCGNT NC
Sbjct: 372 HKRGIEVIMDVVLNHTAEGNEKGP-IFSFRGVDNSVYYM--LAPKGEFYNYSGCGNTFNC 431
Query: 456 NHPVVMELILDSLRHWVVEYHVDGFRFDLASVLCR-----------GPD--------GAP 515
NHPVV + ILD LR+WV E HVDGFRFDL S++ R G D G P
Sbjct: 432 NHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTP 491
Query: 516 LSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKG 575
+S PP+I IS D IL K+IAE WD GGLY VG FP+W W+EWNG +RD +R+FIKG
Sbjct: 492 ISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKG 551
Query: 576 DCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGN 635
G G+FA + GS +LY+ RKP+H INF+ AHDGFTL DLV+YN K+N ANGE N
Sbjct: 552 TDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENN 611
Query: 636 DGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNN 695
DG N N+SWNCG EG+ +++ LR RQM+NF ++LM SQG PM+ MGDEYGHT+ GNN
Sbjct: 612 DGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNN 671
Query: 696 NSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHE--- 739
N+Y HD +N+F W + E D FRF +IKFR + L +F + WH
Sbjct: 672 NTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESL-GLNDFPTAKRLQWHGLAP 695
BLAST of Carg02987 vs. ExPASy Swiss-Prot
Match:
P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)
HSP 1 Score: 436.4 bits (1121), Expect = 6.4e-121
Identity = 280/673 (41.60%), Postives = 358/673 (53.19%), Query Frame = 0
Query: 81 APENLDTGPMLKVV-PGQAFPLGVSEVDNGINFAIFSQHATAVTICLSFNERIDDRTLEF 140
A E+ TGP L V PG A+PLG + G NF++FS+ A V +CL ID+ +E
Sbjct: 6 AGESDGTGPALPTVWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCL-----IDEDGVES 65
Query: 141 KLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLILAVTYKLYGTVLWPLDLKM 200
++ P D G +WH + P+ +
Sbjct: 66 RI-PLDEVDGYVWHAYL------------------PNIT--------------------- 125
Query: 201 DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDF 260
YG+R+ GP GHR D S +LLDPY K G F G+ S + D
Sbjct: 126 ----PGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTF-GQALYSYDVNAVDP 185
Query: 261 DSLP------------------FDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLASS 320
DS P FDW D + P + VIYE +V+ T S +
Sbjct: 186 DSTPPMVDSLGHTMTSVVINPFFDWAYD-RSPRTPYHETVIYEAHVKGMTQTHPS-IPPE 245
Query: 321 IRGSYLGVIEK--IPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFF 380
+RG+Y G+ I HL EL V AVEL+PV +F RN+ WGY+T FF
Sbjct: 246 LRGTYAGLAHPVIIDHLNELNVTAVELMPVHQFLHDSRLLDLGLRNY----WGYNTFGFF 305
Query: 381 APMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGID 440
AP +YAS +A EFK MV++LH AGIEVILDVVYNHT E + + P T +FRGID
Sbjct: 306 APHHQYASTRQAG-SAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGP-TINFRGID 365
Query: 441 NKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVL 500
N YY L + + +F+G GN+LN HP ++LI+DSLR+WV+E HVDGFRFDLAS L
Sbjct: 366 NTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGFRFDLASTL 425
Query: 501 CRG-PDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCG-GLYLVGRFPNWDRWAEWNGIY 560
R D LSA + +D ++S+ K+IAEPWD G G Y VG FP W EWNG Y
Sbjct: 426 ARELHDVDRLSA--FFDLVQQDPVVSQVKLIAEPWDVGEGGYQVGNFPG--LWTEWNGKY 485
Query: 561 RDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVK 620
RD +R + +G+ G FA+R++GSSDLY+ R+P INFV AHDGFTL DLVSYN K
Sbjct: 486 RDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLNDLVSYNDK 545
Query: 621 HNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGD 680
HN ANGE DG + N SWNCG EG T+D +I ALR+RQM+N LM SQGTPM+ GD
Sbjct: 546 HNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRNMWATLMVSQGTPMIAHGD 605
Query: 681 EYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGE 723
E G T+YGNNN Y D+ L+ W ++ + D F + R+ H R F GE
Sbjct: 606 EIGRTQYGNNNVYCQDSELSWMDWSLVD-KNADLLAFARKATTLRKNHKVFRRRRFFEGE 615
BLAST of Carg02987 vs. ExPASy TrEMBL
Match:
A0A6J1F9D1 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443282 PE=4 SV=1)
HSP 1 Score: 1445.3 bits (3740), Expect = 0.0e+00
Identity = 696/738 (94.31%), Postives = 696/738 (94.31%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV
Sbjct: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG
Sbjct: 181 -------------------DLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA
Sbjct: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN
Sbjct: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI
Sbjct: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 696
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 696
BLAST of Carg02987 vs. ExPASy TrEMBL
Match:
A0A6J1IKM9 (isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476487 PE=4 SV=1)
HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 688/738 (93.22%), Postives = 691/738 (93.63%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKR PG SSSSQEVFSETKQGGV
Sbjct: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRGPG--SSSSQEVFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
KICTVSAFGRRDQD VLEQEAPENLDTGPMLK+VPGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KICTVSAFGRRDQDHVLEQEAPENLDTGPMLKIVPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DLPRKNVLYGYRIDGPQGWHHGHRFDD IVLLDPYAKFVEG
Sbjct: 181 -------------------DLPRKNVLYGYRIDGPQGWHHGHRFDDRIVLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS
Sbjct: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
S+RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA
Sbjct: 301 SLRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN
Sbjct: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFH+ALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHMALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI
Sbjct: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 694
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 694
BLAST of Carg02987 vs. ExPASy TrEMBL
Match:
A0A5A7TAT5 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 PE=4 SV=1)
HSP 1 Score: 1326.2 bits (3431), Expect = 0.0e+00
Identity = 634/738 (85.91%), Postives = 658/738 (89.16%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRS+PLHHS LQ PLF SSCTGA H + FRLK Q K GSSSS S+E+FSETKQGGV
Sbjct: 1 MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
K+ TVSAF RRDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
+VTICLS + RIDD LEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DL RKNVLYGYRIDGPQGWHHGHR+DD +LLDPYAKFVEG
Sbjct: 181 ------------------QDLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RR+FGGENKSSGFLGTYDF+SLPFDWG+DYKLPNI EKDLVIYEMNVR FTADESSGL S
Sbjct: 241 RRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
S RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR+HMINTWGYSTINFFA
Sbjct: 301 STRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNAS+EFK+MVKALH AGIEVILDVVYNHTNE DDV PYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDL+NNGQ+ NFSGCGNTLNCNHPVVMELIL+SLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RG DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRV+GSSDLY VN RKPYHGINFVIAHDGFTLRDLVSYNVKHN+
Sbjct: 541 IRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHND 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETED +I+ALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHD ALNHFLW QLEARKRDHFRFFSEVIKFRRKHPHLFSRENFL +NDI
Sbjct: 661 HTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDI 697
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 697
BLAST of Carg02987 vs. ExPASy TrEMBL
Match:
A0A1S3CS22 (isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 PE=4 SV=1)
HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 634/738 (85.91%), Postives = 658/738 (89.16%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRS+PLHHS LQ PLF SSCTGA H + FRLK Q K GSSSS S+E+FSETKQGGV
Sbjct: 1 MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
K+ TVSAF RRDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
+VTICLS + RIDD LEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DL RKNVLYGYRIDGPQGWHHGHR+DD +LLDPYAKFVEG
Sbjct: 181 -------------------DLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RR+FGGENKSSGFLGTYDF+SLPFDWG+DYKLPNI EKDLVIYEMNVR FTADESSGL S
Sbjct: 241 RRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
S RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR+HMINTWGYSTINFFA
Sbjct: 301 STRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNAS+EFK+MVKALH AGIEVILDVVYNHTNE DDV PYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDL+NNGQ+ NFSGCGNTLNCNHPVVMELIL+SLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RG DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRV+GSSDLY VN RKPYHGINFVIAHDGFTLRDLVSYNVKHN+
Sbjct: 541 IRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHND 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETED +I+ALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHD ALNHFLW QLEARKRDHFRFFSEVIKFRRKHPHLFSRENFL +NDI
Sbjct: 661 HTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDI 696
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 696
BLAST of Carg02987 vs. ExPASy TrEMBL
Match:
A0A5D3E5B0 (Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 PE=4 SV=1)
HSP 1 Score: 1325.8 bits (3430), Expect = 0.0e+00
Identity = 634/738 (85.91%), Postives = 658/738 (89.16%), Query Frame = 0
Query: 1 MIRSIPLHHSAPLQLPLFASSCTGAAHSVQFRLKPQGKRAPGSSSSSSQEVFSETKQGGV 60
MIRS+PLHHS LQ PLF SSCTGA H + FRLK Q K GSSSS S+E+FSETKQGGV
Sbjct: 1 MIRSLPLHHSTTLQFPLFPSSCTGATHFLHFRLKRQVKLVSGSSSSDSREIFSETKQGGV 60
Query: 61 KICTVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHAT 120
K+ TVSAF RRDQDRVLEQEAPENLDTGPMLK+ PGQAFPLGVSEVDNGINFAIFSQHAT
Sbjct: 61 KLWTVSAFSRRDQDRVLEQEAPENLDTGPMLKIFPGQAFPLGVSEVDNGINFAIFSQHAT 120
Query: 121 AVTICLSFNERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSSLI 180
+VTICLS + RIDD LEFKLDPDDNRTGDIWHICIQ
Sbjct: 121 SVTICLSLDGRIDDGMLEFKLDPDDNRTGDIWHICIQ----------------------- 180
Query: 181 LAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFVEG 240
DL RKNVLYGYRIDGPQGWHHGHR+DD +LLDPYAKFVEG
Sbjct: 181 -------------------DLQRKNVLYGYRIDGPQGWHHGHRYDDGTMLLDPYAKFVEG 240
Query: 241 RRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSGLAS 300
RR+FGGENKSSGFLGTYDF+SLPFDWG+DYKLPNI EKDLVIYEMNVR FTADESSGL S
Sbjct: 241 RRFFGGENKSSGFLGTYDFESLPFDWGNDYKLPNIPEKDLVIYEMNVRGFTADESSGLPS 300
Query: 301 SIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTINFFA 360
S RGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPR+HMINTWGYSTINFFA
Sbjct: 301 STRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRDHMINTWGYSTINFFA 360
Query: 361 PMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDN 420
PMSRYASAGGGPLNAS+EFK+MVKALH AGIEVILDVVYNHTNE DDV PYTTSFRGIDN
Sbjct: 361 PMSRYASAGGGPLNASQEFKQMVKALHAAGIEVILDVVYNHTNEGDDVNPYTTSFRGIDN 420
Query: 421 KVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLC 480
KVYYMLDL+NNGQ+ NFSGCGNTLNCNHPVVMELIL+SLRHWVVEYHVDGFRFDLASVLC
Sbjct: 421 KVYYMLDLKNNGQYFNFSGCGNTLNCNHPVVMELILESLRHWVVEYHVDGFRFDLASVLC 480
Query: 481 RGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
RG DGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD
Sbjct: 481 RGTDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDD 540
Query: 541 IRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNN 600
IRKFIKGDCGMKGSFATRV+GSSDLY VN RKPYHGINFVIAHDGFTLRDLVSYNVKHN+
Sbjct: 541 IRKFIKGDCGMKGSFATRVAGSSDLYNVNKRKPYHGINFVIAHDGFTLRDLVSYNVKHND 600
Query: 601 ANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
ANGEGGNDGCNDNFSWNCGFEGETED +I+ALRSRQMKNFHLALMTSQGTPMMLMGDEYG
Sbjct: 601 ANGEGGNDGCNDNFSWNCGFEGETEDTSIKALRSRQMKNFHLALMTSQGTPMMLMGDEYG 660
Query: 661 HTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDI 720
HTRYGNNNSYGHD ALNHFLW QLEARKRDHFRFFSEVIKFRRKHPHLFSRENFL +NDI
Sbjct: 661 HTRYGNNNSYGHDNALNHFLWEQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLNKNDI 696
Query: 721 TWHESNWDNPESKFLAYT 739
TWHESNWDNPESKFLAYT
Sbjct: 721 TWHESNWDNPESKFLAYT 696
BLAST of Carg02987 vs. TAIR 10
Match:
AT4G09020.1 (isoamylase 3 )
HSP 1 Score: 1014.2 bits (2621), Expect = 5.2e-296
Identity = 487/681 (71.51%), Postives = 544/681 (79.88%), Query Frame = 0
Query: 64 TVSAFGRRDQDRVLEQEAPENLDTGPMLKVVPGQAFPLGVSEVDNGINFAIFSQHATAVT 123
T+ RR +RV+E+EA +T + KV G+ PLGVS+VD GINFA+FSQ+AT+VT
Sbjct: 57 TLKVTCRRAHERVVEEEASTMTET-KLFKVSSGEVSPLGVSQVDKGINFALFSQNATSVT 116
Query: 124 ICLSF-----NERIDDRTLEFKLDPDDNRTGDIWHICIQFTLIILHSPFGSSVHRKPSSS 183
+CLS ++ DD +E LDP N+TGD WHIC++
Sbjct: 117 LCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVE--------------------- 176
Query: 184 LILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRIDGPQGWHHGHRFDDSIVLLDPYAKFV 243
DLP NVLYGYR+DGP W GHRFD SI+LLDPYAK V
Sbjct: 177 ---------------------DLPLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLV 236
Query: 244 EGRRYFG-GENKSSGFLGTYDFDSLPFDWGDDYKLPNILEKDLVIYEMNVRAFTADESSG 303
+G FG K + F GTYDF+S PFDWGDDYK PNI EKDLVIYEMNVRAFTADESSG
Sbjct: 237 KGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSG 296
Query: 304 LASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDELEFQRHPNPRNHMINTWGYSTIN 363
+ +I GSYLG IEKIPHL +LG+NAVELLPVFEFDELE QR NPR+HM+NTWGYST+N
Sbjct: 297 MDPAIGGSYLGFIEKIPHLQDLGINAVELLPVFEFDELELQRRSNPRDHMVNTWGYSTVN 356
Query: 364 FFAPMSRYASAGGGPLNASREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRG 423
FFAPMSRYAS G P+ AS+EFKEMVKALH AGIEVILDVVYNHTNEADD YPYTTSFRG
Sbjct: 357 FFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRG 416
Query: 424 IDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLAS 483
IDNKVYYMLD N Q LNFSGCGNTLNCNHPVVMELILDSLRHWV EYHVDGFRFDLAS
Sbjct: 417 IDNKVYYMLD--PNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLAS 476
Query: 484 VLCRGPDGAPLSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIY 543
VLCR DG+PLSAPPLIRAI+KD++LSRCKIIAEPWDCGGLYLVG+FPNWDRWAEWNG+Y
Sbjct: 477 VLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPNWDRWAEWNGMY 536
Query: 544 RDDIRKFIKGDCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVK 603
RDD+R+FIKGD GMKGSFATRVSGSSDLY+VN RKPYHG+NFVIAHDGFTLRDLVSYN K
Sbjct: 537 RDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFK 596
Query: 604 HNNANGEGGNDGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGD 663
HN ANGEGGNDGCNDN SWNCGFEGET DA+I++LR+RQMKNFHLALM SQGTPMMLMGD
Sbjct: 597 HNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGD 656
Query: 664 EYGHTRYGNNNSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGE 723
EYGHTRYGNNNSYGHDT+LN+F W +L+A+K++HFRFFSEVIKFR H H+ ENFL +
Sbjct: 657 EYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSH-HVLKHENFLTQ 691
Query: 724 NDITWHESNWDNPESKFLAYT 739
+ITWHE NWDN ESKFLA+T
Sbjct: 717 GEITWHEDNWDNSESKFLAFT 691
BLAST of Carg02987 vs. TAIR 10
Match:
AT2G39930.1 (isoamylase 1 )
HSP 1 Score: 546.6 bits (1407), Expect = 3.1e-155
Identity = 308/676 (45.56%), Postives = 394/676 (58.28%), Query Frame = 0
Query: 96 GQAFPLGVSEVDNGINFAIFSQHATAVTICL-SFNE-RIDDRTLEFKLDPDDNRTGDIWH 155
G P G + D+G+NF+++S ++ + TICL S ++ R + T E +LDP NRTG +WH
Sbjct: 72 GLPSPFGPTVRDDGVNFSVYSTNSVSATICLISLSDLRQNKVTEEIQLDPSRNRTGHVWH 131
Query: 156 ICIQFTLIILHSPFGSSVHRKPSSSLILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRID 215
+ L F K++LYGYR D
Sbjct: 132 -------VFLRGDF------------------------------------KDMLYGYRFD 191
Query: 216 GPQGWHHGHRFDDSIVLLDPYAKFVEGRRYFG----GENKSSGFLGTYDFDSLPFDWGDD 275
G GH +D S +LLDPYAK + R FG +N FDW D
Sbjct: 192 GKFSPEEGHYYDSSNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEEFDWEGD 251
Query: 276 YKLPNILEKDLVIYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPV 335
L + +KDLVIYEM+VR FT ESS + G+Y GV EK+ HL ELG+N +EL+P
Sbjct: 252 MHL-KLPQKDLVIYEMHVRGFTRHESSKI--EFPGTYQGVAEKLDHLKELGINCIELMPC 311
Query: 336 FEFDELEFQRHPN-PRNHMINTWGYSTINFFAPMSRYASAGGGPL--NASREFKEMVKAL 395
EF+ELE+ + +H +N WGYSTI FF+PM RYASA A EFK +VK
Sbjct: 312 HEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEA 371
Query: 396 HGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNC 455
H GIEVI+DVV NHT E ++ P SFRG+DN VYYM L G+F N+SGCGNT NC
Sbjct: 372 HKRGIEVIMDVVLNHTAEGNEKGP-IFSFRGVDNSVYYM--LAPKGEFYNYSGCGNTFNC 431
Query: 456 NHPVVMELILDSLRHWVVEYHVDGFRFDLASVLCR-----------GPD--------GAP 515
NHPVV + ILD LR+WV E HVDGFRFDL S++ R G D G P
Sbjct: 432 NHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTP 491
Query: 516 LSAPPLIRAISKDAILSRCKIIAEPWDCGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKG 575
+S PP+I IS D IL K+IAE WD GGLY VG FP+W W+EWNG +RD +R+FIKG
Sbjct: 492 ISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGMFPHWGIWSEWNGKFRDVVRQFIKG 551
Query: 576 DCGMKGSFATRVSGSSDLYKVNNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGN 635
G G+FA + GS +LY+ RKP+H INF+ AHDGFTL DLV+YN K+N ANGE N
Sbjct: 552 TDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAHDGFTLADLVTYNNKNNLANGEENN 611
Query: 636 DGCNDNFSWNCGFEGETEDANIRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNN 695
DG N N+SWNCG EG+ +++ LR RQM+NF ++LM SQG PM+ MGDEYGHT+ GNN
Sbjct: 612 DGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGDEYGHTKGGNN 671
Query: 696 NSYGHDTALNHFLWGQLEARKRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHE--- 739
N+Y HD +N+F W + E D FRF +IKFR + L +F + WH
Sbjct: 672 NTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRDECESL-GLNDFPTAKRLQWHGLAP 695
BLAST of Carg02987 vs. TAIR 10
Match:
AT1G03310.2 (debranching enzyme 1 )
HSP 1 Score: 325.1 bits (832), Expect = 1.5e-88
Identity = 224/654 (34.25%), Postives = 316/654 (48.32%), Query Frame = 0
Query: 96 GQAFPLGVSE--VDNGINFAIFSQHATAVTICLSFNERIDDRTLEFKLDPDDNRTGDIWH 155
G PLG+S D+ NF+ FS+ +T V +CL + D LE LDP NRTGD+WH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289
Query: 156 ICIQFTLIILHSPFGSSVHRKPSSSLILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRID 215
+ T W V YGYR
Sbjct: 290 ASVDNT---------------------------------WDF----------VRYGYRCK 349
Query: 216 GPQGWHHGHRFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLP 275
+ ++LDPYA V G++ S +LG+ S FDWG+D P
Sbjct: 350 ETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLGSLS-KSPSFDWGEDVS-P 409
Query: 276 NILEKDLVIYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFD 335
NI + L++Y +NV+ FT SS L S++ G++ GV EK+ HL LG NAV L P+F F
Sbjct: 410 NIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFS 469
Query: 336 ELEFQRHPNPRNHMINTWGYSTINFFAPMSRYASAGGGPLN----ASREFKEMVKALHGA 395
E Q+ P Y +FF+PM Y GP N A K MVK LH
Sbjct: 470 E---QKGP-----------YFPFHFFSPMDIY-----GPSNSLESAVNSMKVMVKKLHSE 529
Query: 396 GIEVILDVVYNHTNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHP 455
GIEV+L+VV+ HT ++ + RGID+ YY N+ ++ LNCN+P
Sbjct: 530 GIEVLLEVVFTHTADSG-------ALRGIDDSSYYYKGRANDLDSKSY------LNCNYP 589
Query: 456 VVMELILDSLRHWVVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKII 515
VV +L+L+SLR+WV E+HVDGF F AS L RG G LS PPL+ AI+ D +L+ K+I
Sbjct: 590 VVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLI 649
Query: 516 AEPWD-CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKV 575
A+ WD + RFP+W RWAE N Y ++R F++G G+ ATR+ GS D++
Sbjct: 650 ADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVF-T 709
Query: 576 NNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDAN 635
+ R P N++ + G +L D+VS++ SWNCG EG T +
Sbjct: 710 DGRGPAFSFNYISRNSGLSLVDIVSFS-----------GPELASELSWNCGEEGATNKSA 769
Query: 636 IRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQL-EAR 695
+ R +Q++NF S G P++ MGDE G + G+ + F W L A
Sbjct: 770 VLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLL----ESRKPFDWNLLASAF 782
Query: 696 KRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHESN-----WDNPESKFLA 737
+F S + R + +F R +FL +I W+ ++ W++P SKFLA
Sbjct: 830 GTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLA 782
BLAST of Carg02987 vs. TAIR 10
Match:
AT1G03310.1 (debranching enzyme 1 )
HSP 1 Score: 325.1 bits (832), Expect = 1.5e-88
Identity = 224/654 (34.25%), Postives = 316/654 (48.32%), Query Frame = 0
Query: 96 GQAFPLGVSE--VDNGINFAIFSQHATAVTICLSFNERIDDRTLEFKLDPDDNRTGDIWH 155
G PLG+S D+ NF+ FS+ +T V +CL + D LE LDP NRTGD+WH
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELDLDPYVNRTGDVWH 289
Query: 156 ICIQFTLIILHSPFGSSVHRKPSSSLILAVTYKLYGTVLWPLDLKMDLPRKNVLYGYRID 215
+ T W V YGYR
Sbjct: 290 ASVDNT---------------------------------WDF----------VRYGYRCK 349
Query: 216 GPQGWHHGHRFDDSIVLLDPYAKFVEGRRYFGGENKSSGFLGTYDFDSLPFDWGDDYKLP 275
+ ++LDPYA V G++ S +LG+ S FDWG+D P
Sbjct: 350 ETAHSKEDVDVEGEPIVLDPYATVV-------GKSVSQKYLGSLS-KSPSFDWGEDVS-P 409
Query: 276 NILEKDLVIYEMNVRAFTADESSGLASSIRGSYLGVIEKIPHLLELGVNAVELLPVFEFD 335
NI + L++Y +NV+ FT SS L S++ G++ GV EK+ HL LG NAV L P+F F
Sbjct: 410 NIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPIFSFS 469
Query: 336 ELEFQRHPNPRNHMINTWGYSTINFFAPMSRYASAGGGPLN----ASREFKEMVKALHGA 395
E Q+ P Y +FF+PM Y GP N A K MVK LH
Sbjct: 470 E---QKGP-----------YFPFHFFSPMDIY-----GPSNSLESAVNSMKVMVKKLHSE 529
Query: 396 GIEVILDVVYNHTNEADDVYPYTTSFRGIDNKVYYMLDLRNNGQFLNFSGCGNTLNCNHP 455
GIEV+L+VV+ HT ++ + RGID+ YY N+ ++ LNCN+P
Sbjct: 530 GIEVLLEVVFTHTADSG-------ALRGIDDSSYYYKGRANDLDSKSY------LNCNYP 589
Query: 456 VVMELILDSLRHWVVEYHVDGFRFDLASVLCRGPDGAPLSAPPLIRAISKDAILSRCKII 515
VV +L+L+SLR+WV E+HVDGF F AS L RG G LS PPL+ AI+ D +L+ K+I
Sbjct: 590 VVQQLVLESLRYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLI 649
Query: 516 AEPWD-CGGLYLVGRFPNWDRWAEWNGIYRDDIRKFIKGDCGMKGSFATRVSGSSDLYKV 575
A+ WD + RFP+W RWAE N Y ++R F++G G+ ATR+ GS D++
Sbjct: 650 ADCWDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRGR-GVLSDLATRICGSGDVF-T 709
Query: 576 NNRKPYHGINFVIAHDGFTLRDLVSYNVKHNNANGEGGNDGCNDNFSWNCGFEGETEDAN 635
+ R P N++ + G +L D+VS++ SWNCG EG T +
Sbjct: 710 DGRGPAFSFNYISRNSGLSLVDIVSFS-----------GPELASELSWNCGEEGATNKSA 769
Query: 636 IRALRSRQMKNFHLALMTSQGTPMMLMGDEYGHTRYGNNNSYGHDTALNHFLWGQL-EAR 695
+ R +Q++NF S G P++ MGDE G + G+ + F W L A
Sbjct: 770 VLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLL----ESRKPFDWNLLASAF 782
Query: 696 KRDHFRFFSEVIKFRRKHPHLFSRENFLGENDITWHESN-----WDNPESKFLA 737
+F S + R + +F R +FL +I W+ ++ W++P SKFLA
Sbjct: 830 GTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLA 782
BLAST of Carg02987 vs. TAIR 10
Match:
AT5G04360.1 (limit dextrinase )
HSP 1 Score: 112.1 bits (279), Expect = 1.9e-24
Identity = 99/331 (29.91%), Postives = 149/331 (45.02%), Query Frame = 0
Query: 232 DPYAK--FVEGRRYFGGENKSSGFLGTYDFDSL-PFDWGD--DYKLPNILEKDLVIYEMN 291
DPYA+ +GR+ FL D D L P W + D K D+ IYE++
Sbjct: 289 DPYARGLSADGRK---------TFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELH 348
Query: 292 VRAFTADESSGLASSIRGSYLGVIEK-------IPHLLELGVNAVELLPVFEFDELEFQ- 351
VR F+A++ + + RG YL K + L++ G+ + LLP F+F +++ +
Sbjct: 349 VRDFSANDET-VEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEK 408
Query: 352 -----------------------RHPNPRNHMINTWGYSTINFFAPMSRYASAGGGPLNA 411
R +N WGY+ + + P YAS GP
Sbjct: 409 ENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRI 468
Query: 412 SREFKEMVKALHGAGIEVILDVVYNHTNEADDVYPYTTSFRGIDNKV---YYMLDLRNNG 471
EF++MV+AL+ G+ V+LDVVYNH + + P+ + +K+ YY+ RN+
Sbjct: 469 I-EFRKMVQALNCTGLNVVLDVVYNHLHASG---PHDK--ESVLDKIVPGYYL--RRNSD 528
Query: 472 QFLNFSGCGNTLNCNHPVVMELILDSLRHWVVEYHVDGFRFDLASVLCRGPDGAPLSAPP 524
F+ S C N H +V LI D L +WVV Y VDGFRFDL + + SA
Sbjct: 529 GFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIG 588
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7024560.1 | 0.0e+00 | 100.00 | Isoamylase 3, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
XP_022936809.1 | 0.0e+00 | 94.31 | isoamylase 3, chloroplastic isoform X1 [Cucurbita moschata] | [more] |
XP_023535183.1 | 0.0e+00 | 93.50 | isoamylase 3, chloroplastic [Cucurbita pepo subsp. pepo] | [more] |
XP_022975928.1 | 0.0e+00 | 93.22 | isoamylase 3, chloroplastic isoform X1 [Cucurbita maxima] | [more] |
XP_038896292.1 | 0.0e+00 | 86.99 | isoamylase 3, chloroplastic isoform X1 [Benincasa hispida] >XP_038896293.1 isoam... | [more] |
Match Name | E-value | Identity | Description | |
Q9M0S5 | 7.4e-295 | 71.51 | Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2 | [more] |
B9G434 | 3.0e-288 | 71.47 | Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... | [more] |
D0TZF0 | 3.0e-155 | 44.51 | Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... | [more] |
O04196 | 4.4e-154 | 45.56 | Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1 | [more] |
P0A4Y5 | 6.4e-121 | 41.60 | Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1F9D1 | 0.0e+00 | 94.31 | isoamylase 3, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11144... | [more] |
A0A6J1IKM9 | 0.0e+00 | 93.22 | isoamylase 3, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114764... | [more] |
A0A5A7TAT5 | 0.0e+00 | 85.91 | Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold92G002290 P... | [more] |
A0A1S3CS22 | 0.0e+00 | 85.91 | isoamylase 3, chloroplastic isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503684 P... | [more] |
A0A5D3E5B0 | 0.0e+00 | 85.91 | Isoamylase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005950 ... | [more] |