Carg02726 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02726
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAP2-like ethylene-responsive transcription factor PLT2
LocationCarg_Chr02: 7183833 .. 7184423 (-)
RNA-Seq ExpressionCarg02726
SyntenyCarg02726
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAATCCACCAATTTGATTGTTGTTGGGGGCTTTCACGGGTGCGAAAACCAATTGGGCGGTCAACAGATTGAAGCATGGGGAGTCCCGCTAAATCTTTTGAGAGATATTCACTAATCTTTCCTTTTACCCTCTTCTTCTTCCCTGTTTTACCTCTGTTCCTGAAGAAAATGGGGTCCATCAATTCAAACAATTGCCTTTCCTGTCCACTTCCACCCACTAACCCTTCATCCTCCTTGCCGGAAGTCCCATTTCAAGCCCCCAATGGTATGTATGTTTATACATTCACAAATCCCACTTGCATATCAATCTTGGATTCATCATTGTTTGTCTCTGTTTTGTAGAGTGGAATCTGATAAGTAGTGAAGGCGGTGGTGAAGCTCCAAAAGTTGCAGACTTTTTAGGCGTAAGCAAGGCAGAAAATGAGACACATCTCATGGGATTTAATGAAATTCATGCAGATTACTTGTTCCCAATCACACCATTTAATGTGGACTCCACCACTACCAATTCAAATAGTCTGCAATGTTTGAATCTTTCAATGGGGAGTGGGAAGGGCGAACCCCCCCCCCCCCCCCCCCCACCACTTTAA

mRNA sequence

GTTAATCCACCAATTTGATTGTTGTTGGGGGCTTTCACGGGTGCGAAAACCAATTGGGCGGTCAACAGATTGAAGCATGGGGAGTCCCGCTAAATCTTTTGAGAGATATTCACTAATCTTTCCTTTTACCCTCTTCTTCTTCCCTGTTTTACCTCTGTTCCTGAAGAAAATGGGGTCCATCAATTCAAACAATTGCCTTTCCTGTCCACTTCCACCCACTAACCCTTCATCCTCCTTGCCGGAAGTCCCATTTCAAGCCCCCAATGAGTGGAATCTGATAAGTAGTGAAGGCGGTGGTGAAGCTCCAAAAGTTGCAGACTTTTTAGGCGTAAGCAAGGCAGAAAATGAGACACATCTCATGGGATTTAATGAAATTCATGCAGATTACTTGTTCCCAATCACACCATTTAATGTGGACTCCACCACTACCAATTCAAATAGTCTGCAATGTTTGAATCTTTCAATGGGGAGTGGGAAGGGCGAACCCCCCCCCCCCCCCCCCCCACCACTTTAA

Coding sequence (CDS)

ATGGGGAGTCCCGCTAAATCTTTTGAGAGATATTCACTAATCTTTCCTTTTACCCTCTTCTTCTTCCCTGTTTTACCTCTGTTCCTGAAGAAAATGGGGTCCATCAATTCAAACAATTGCCTTTCCTGTCCACTTCCACCCACTAACCCTTCATCCTCCTTGCCGGAAGTCCCATTTCAAGCCCCCAATGAGTGGAATCTGATAAGTAGTGAAGGCGGTGGTGAAGCTCCAAAAGTTGCAGACTTTTTAGGCGTAAGCAAGGCAGAAAATGAGACACATCTCATGGGATTTAATGAAATTCATGCAGATTACTTGTTCCCAATCACACCATTTAATGTGGACTCCACCACTACCAATTCAAATAGTCTGCAATGTTTGAATCTTTCAATGGGGAGTGGGAAGGGCGAACCCCCCCCCCCCCCCCCCCCACCACTTTAA

Protein sequence

MGSPAKSFERYSLIFPFTLFFFPVLPLFLKKMGSINSNNCLSCPLPPTNPSSSLPEVPFQAPNEWNLISSEGGGEAPKVADFLGVSKAENETHLMGFNEIHADYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEPPPPPPPPL
Homology
BLAST of Carg02726 vs. NCBI nr
Match: KAG7035846.1 (AP2-like ethylene-responsive transcription factor PLT1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 299.7 bits (766), Expect = 1.4e-77
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0

Query: 1   MGSPAKSFERYSLIFPFTLFFFPVLPLFLKKMGSINSNNCLSCPLPPTNPSSSLPEVPFQ 60
           MGSPAKSFERYSLIFPFTLFFFPVLPLFLKKMGSINSNNCLSCPLPPTNPSSSLPEVPFQ
Sbjct: 1   MGSPAKSFERYSLIFPFTLFFFPVLPLFLKKMGSINSNNCLSCPLPPTNPSSSLPEVPFQ 60

Query: 61  APNEWNLISSEGGGEAPKVADFLGVSKAENETHLMGFNEIHADYLFPITPFNVDSTTTNS 120
           APNEWNLISSEGGGEAPKVADFLGVSKAENETHLMGFNEIHADYLFPITPFNVDSTTTNS
Sbjct: 61  APNEWNLISSEGGGEAPKVADFLGVSKAENETHLMGFNEIHADYLFPITPFNVDSTTTNS 120

Query: 121 NSLQCLNLSMGSGKGEPPPPPPPPL 146
           NSLQCLNLSMGSGKGEPPPPPPPPL
Sbjct: 121 NSLQCLNLSMGSGKGEPPPPPPPPL 145

BLAST of Carg02726 vs. NCBI nr
Match: XP_022958273.1 (LOW QUALITY PROTEIN: AP2-like ethylene-responsive transcription factor PLT1 [Cucurbita moschata])

HSP 1 Score: 214.2 bits (544), Expect = 7.7e-52
Identity = 102/110 (92.73%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPSSSLPEVPFQAPNEWNLISSEGGGEAPKVADFLGVSKAENE 91
           MGS+NSNNCLS PL PTNPS SLPE+PFQAPNEWNLIS+EGGGEAPKVADFLGVSKAENE
Sbjct: 1   MGSMNSNNCLSFPLSPTNPSFSLPEIPFQAPNEWNLISTEGGGEAPKVADFLGVSKAENE 60

Query: 92  THLMGFNEIHADYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEPPPPP 142
           THLMGFNEIH DYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEP PPP
Sbjct: 61  THLMGFNEIHTDYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEPTPPP 110

BLAST of Carg02726 vs. NCBI nr
Match: KAG6605902.1 (AP2-like ethylene-responsive transcription factor PLT2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 198.4 bits (503), Expect = 4.4e-47
Identity = 94/100 (94.00%), Postives = 96/100 (96.00%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPSSSLPEVPFQAPNEWNLISSEGGGEAPKVADFLGVSKAENE 91
           MGSINSNNCLSCPLPPTNPSSSLPEVPFQAPNEWNLISSEGGGEAPKVADFLGVSKAENE
Sbjct: 1   MGSINSNNCLSCPLPPTNPSSSLPEVPFQAPNEWNLISSEGGGEAPKVADFLGVSKAENE 60

Query: 92  THLMGFNEIHADYLFPITPFNVDSTTTNSNSLQCLNLSMG 132
           THLMGFNEIHADYLFPITPFNVDSTTTNSN LQ + +S G
Sbjct: 61  THLMGFNEIHADYLFPITPFNVDSTTTNSNKLQSIAVSPG 100

BLAST of Carg02726 vs. NCBI nr
Match: XP_022944693.1 (AP2-like ethylene-responsive transcription factor PLT2 [Cucurbita moschata])

HSP 1 Score: 124.4 bits (311), Expect = 8.0e-25
Identity = 77/146 (52.74%), Postives = 86/146 (58.90%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPS--------------------SSLPEVPFQAPNEWNLISSE 91
           MGS+NSNN LS PL PTNPS                    +   ++PFQ  +EWNLIS +
Sbjct: 1   MGSMNSNNWLSFPLSPTNPSLPPHLQTPTHAHHFSLGNLENDNMDIPFQT-HEWNLISGQ 60

Query: 92  GGGEAPKVADFLGVSKAENETHLMGFNEIH----ADYLFPIT--------------PFNV 135
           GGGE PKVADFLGVSKAENE  L+GFN+IH     DYLFPIT              P N+
Sbjct: 61  GGGEVPKVADFLGVSKAENEADLIGFNQIHEANDTDYLFPITRLVPLQQHPTPAAAPINM 120

BLAST of Carg02726 vs. NCBI nr
Match: XP_022985835.1 (AP2-like ethylene-responsive transcription factor PLT2 [Cucurbita maxima])

HSP 1 Score: 124.4 bits (311), Expect = 8.0e-25
Identity = 77/146 (52.74%), Postives = 86/146 (58.90%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPS--------------------SSLPEVPFQAPNEWNLISSE 91
           MGS+NSNN LS PL PTNPS                    +   ++PFQ  +EWNLIS +
Sbjct: 1   MGSMNSNNWLSFPLSPTNPSLPPHLQTPTHAHHFSLGNLENDNMDIPFQT-HEWNLISGQ 60

Query: 92  GGGEAPKVADFLGVSKAENETHLMGFNEIH----ADYLFPIT--------------PFNV 135
           GGGE PKVADFLGVSKAENE  L+GFN+IH     DYLFPIT              P N+
Sbjct: 61  GGGEVPKVADFLGVSKAENEADLIGFNQIHEANDTDYLFPITRLVPLQQHPTPAAAPINM 120

BLAST of Carg02726 vs. ExPASy Swiss-Prot
Match: Q5YGP8 (AP2-like ethylene-responsive transcription factor PLT1 OS=Arabidopsis thaliana OX=3702 GN=PLT1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.0e-10
Identity = 57/141 (40.43%), Postives = 70/141 (49.65%), Query Frame = 0

Query: 35  INSNNCLSCPLPPTNPSSSLP-------------EVPFQAPNEWNLISSEG-----GGEA 94
           +NSNN L  PL P N  SSLP             + PFQ   EWN+I+  G     GGE 
Sbjct: 1   MNSNNWLGFPLSPNN--SSLPPHEYNLGLVSDHMDNPFQT-QEWNMINPHGGGGDEGGEV 60

Query: 95  PKVADFLGVSKA-ENET-HLMGFNE----IHADYLFPITPFN------VDSTTTNSNS-- 136
           PKVADFLGVSK  EN++ HL+ +N+     H + L P    N       DS T N++S  
Sbjct: 61  PKVADFLGVSKPDENQSNHLVAYNDSDYYFHTNSLMPSVQSNDVVVAACDSNTPNNSSYH 120

BLAST of Carg02726 vs. ExPASy Swiss-Prot
Match: Q5YGP7 (AP2-like ethylene-responsive transcription factor PLT2 OS=Arabidopsis thaliana OX=3702 GN=PLT2 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.1e-08
Identity = 52/141 (36.88%), Postives = 66/141 (46.81%), Query Frame = 0

Query: 35  INSNNCLSCPLPPTNPSSSLP--------------------EVPFQAPNEWNLISSEG-- 94
           +NSNN L+ PL PT+  SSLP                    + PFQ    WN+I+  G  
Sbjct: 1   MNSNNWLAFPLSPTH--SSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQG-WNMINPHGGG 60

Query: 95  --GGEAPKVADFLGVSKA---ENETHLMGFNEIH----ADYLFPITPFNVDSTTTNSN-- 133
             GGE PKVADFLGVSK+     + +L+ +N+IH    +DY F          T  SN  
Sbjct: 61  GEGGEVPKVADFLGVSKSGDHHTDHNLVPYNDIHQTNASDYYFQTNSLLPTVVTCASNAP 120

BLAST of Carg02726 vs. ExPASy TrEMBL
Match: A0A6J1H2P2 (LOW QUALITY PROTEIN: AP2-like ethylene-responsive transcription factor PLT1 OS=Cucurbita moschata OX=3662 GN=LOC111459548 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-52
Identity = 102/110 (92.73%), Postives = 105/110 (95.45%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPSSSLPEVPFQAPNEWNLISSEGGGEAPKVADFLGVSKAENE 91
           MGS+NSNNCLS PL PTNPS SLPE+PFQAPNEWNLIS+EGGGEAPKVADFLGVSKAENE
Sbjct: 1   MGSMNSNNCLSFPLSPTNPSFSLPEIPFQAPNEWNLISTEGGGEAPKVADFLGVSKAENE 60

Query: 92  THLMGFNEIHADYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEPPPPP 142
           THLMGFNEIH DYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEP PPP
Sbjct: 61  THLMGFNEIHTDYLFPITPFNVDSTTTNSNSLQCLNLSMGSGKGEPTPPP 110

BLAST of Carg02726 vs. ExPASy TrEMBL
Match: A0A6J1JCD5 (AP2-like ethylene-responsive transcription factor PLT2 OS=Cucurbita maxima OX=3661 GN=LOC111483764 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 3.9e-25
Identity = 77/146 (52.74%), Postives = 86/146 (58.90%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPS--------------------SSLPEVPFQAPNEWNLISSE 91
           MGS+NSNN LS PL PTNPS                    +   ++PFQ  +EWNLIS +
Sbjct: 1   MGSMNSNNWLSFPLSPTNPSLPPHLQTPTHAHHFSLGNLENDNMDIPFQT-HEWNLISGQ 60

Query: 92  GGGEAPKVADFLGVSKAENETHLMGFNEIH----ADYLFPIT--------------PFNV 135
           GGGE PKVADFLGVSKAENE  L+GFN+IH     DYLFPIT              P N+
Sbjct: 61  GGGEVPKVADFLGVSKAENEADLIGFNQIHEANDTDYLFPITRLVPLQQHPTPAAAPINM 120

BLAST of Carg02726 vs. ExPASy TrEMBL
Match: A0A6J1FYB3 (AP2-like ethylene-responsive transcription factor PLT2 OS=Cucurbita moschata OX=3662 GN=LOC111449076 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 3.9e-25
Identity = 77/146 (52.74%), Postives = 86/146 (58.90%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPS--------------------SSLPEVPFQAPNEWNLISSE 91
           MGS+NSNN LS PL PTNPS                    +   ++PFQ  +EWNLIS +
Sbjct: 1   MGSMNSNNWLSFPLSPTNPSLPPHLQTPTHAHHFSLGNLENDNMDIPFQT-HEWNLISGQ 60

Query: 92  GGGEAPKVADFLGVSKAENETHLMGFNEIH----ADYLFPIT--------------PFNV 135
           GGGE PKVADFLGVSKAENE  L+GFN+IH     DYLFPIT              P N+
Sbjct: 61  GGGEVPKVADFLGVSKAENEADLIGFNQIHEANDTDYLFPITRLVPLQQHPTPAAAPINM 120

BLAST of Carg02726 vs. ExPASy TrEMBL
Match: A0A1S4E5A7 (AP2-like ethylene-responsive transcription factor PLT2 OS=Cucumis melo OX=3656 GN=LOC103503191 PE=4 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.8e-22
Identity = 76/159 (47.80%), Postives = 88/159 (55.35%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPSSSLP------------------------EVPFQAPNEWNL 91
           MGS+NSNN LS PL PTNP  SLP                        ++PFQ  +EWNL
Sbjct: 1   MGSMNSNNWLSFPLSPTNP--SLPPHLQTTTHHSHHFSLGNLENDHNMDIPFQT-HEWNL 60

Query: 92  ISSEGGGEAPKVADFLGVSKAENETHLMGFNEIH-----ADYLFPIT------------- 135
           IS++GGGE PK+ADFLGVSKAENET L+GFNEIH      DYLFPIT             
Sbjct: 61  ISNQGGGEVPKIADFLGVSKAENETDLIGFNEIHHQSNDTDYLFPITRLVPLHQQQQQQT 120

BLAST of Carg02726 vs. ExPASy TrEMBL
Match: A0A5D3BC19 (AP2-like ethylene-responsive transcription factor PLT2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001210 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.4e-22
Identity = 76/160 (47.50%), Postives = 88/160 (55.00%), Query Frame = 0

Query: 32  MGSINSNNCLSCPLPPTNPSSSLP------------------------EVPFQAPNEWNL 91
           MGS+NSNN LS PL PTNP  SLP                        ++PFQ  +EWNL
Sbjct: 1   MGSMNSNNWLSFPLSPTNP--SLPPHLQTTTHHSHHFSLGNLENDHNMDIPFQT-HEWNL 60

Query: 92  ISSEGGGEAPKVADFLGVSKAENETHLMGFNEIH-----ADYLFPIT------------- 135
           IS++GGGE PK+ADFLGVSKAENET L+GFNEIH      DYLFPIT             
Sbjct: 61  ISNQGGGEVPKIADFLGVSKAENETDLIGFNEIHHQSNDTDYLFPITRLVPLHQQQQQQQ 120

BLAST of Carg02726 vs. TAIR 10
Match: AT3G20840.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.4e-11
Identity = 57/141 (40.43%), Postives = 70/141 (49.65%), Query Frame = 0

Query: 35  INSNNCLSCPLPPTNPSSSLP-------------EVPFQAPNEWNLISSEG-----GGEA 94
           +NSNN L  PL P N  SSLP             + PFQ   EWN+I+  G     GGE 
Sbjct: 1   MNSNNWLGFPLSPNN--SSLPPHEYNLGLVSDHMDNPFQT-QEWNMINPHGGGGDEGGEV 60

Query: 95  PKVADFLGVSKA-ENET-HLMGFNE----IHADYLFPITPFN------VDSTTTNSNS-- 136
           PKVADFLGVSK  EN++ HL+ +N+     H + L P    N       DS T N++S  
Sbjct: 61  PKVADFLGVSKPDENQSNHLVAYNDSDYYFHTNSLMPSVQSNDVVVAACDSNTPNNSSYH 120

BLAST of Carg02726 vs. TAIR 10
Match: AT1G51190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 2.9e-09
Identity = 52/141 (36.88%), Postives = 66/141 (46.81%), Query Frame = 0

Query: 35  INSNNCLSCPLPPTNPSSSLP--------------------EVPFQAPNEWNLISSEG-- 94
           +NSNN L+ PL PT+  SSLP                    + PFQ    WN+I+  G  
Sbjct: 1   MNSNNWLAFPLSPTH--SSLPPHIHSSQNSHFNLGLVNDNIDNPFQNQG-WNMINPHGGG 60

Query: 95  --GGEAPKVADFLGVSKA---ENETHLMGFNEIH----ADYLFPITPFNVDSTTTNSN-- 133
             GGE PKVADFLGVSK+     + +L+ +N+IH    +DY F          T  SN  
Sbjct: 61  GEGGEVPKVADFLGVSKSGDHHTDHNLVPYNDIHQTNASDYYFQTNSLLPTVVTCASNAP 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035846.11.4e-77100.00AP2-like ethylene-responsive transcription factor PLT1 [Cucurbita argyrosperma s... [more]
XP_022958273.17.7e-5292.73LOW QUALITY PROTEIN: AP2-like ethylene-responsive transcription factor PLT1 [Cuc... [more]
KAG6605902.14.4e-4794.00AP2-like ethylene-responsive transcription factor PLT2, partial [Cucurbita argyr... [more]
XP_022944693.18.0e-2552.74AP2-like ethylene-responsive transcription factor PLT2 [Cucurbita moschata][more]
XP_022985835.18.0e-2552.74AP2-like ethylene-responsive transcription factor PLT2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5YGP82.0e-1040.43AP2-like ethylene-responsive transcription factor PLT1 OS=Arabidopsis thaliana O... [more]
Q5YGP74.1e-0836.88AP2-like ethylene-responsive transcription factor PLT2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1H2P23.7e-5292.73LOW QUALITY PROTEIN: AP2-like ethylene-responsive transcription factor PLT1 OS=C... [more]
A0A6J1JCD53.9e-2552.74AP2-like ethylene-responsive transcription factor PLT2 OS=Cucurbita maxima OX=36... [more]
A0A6J1FYB33.9e-2552.74AP2-like ethylene-responsive transcription factor PLT2 OS=Cucurbita moschata OX=... [more]
A0A1S4E5A71.8e-2247.80AP2-like ethylene-responsive transcription factor PLT2 OS=Cucumis melo OX=3656 G... [more]
A0A5D3BC192.4e-2247.50AP2-like ethylene-responsive transcription factor PLT2 OS=Cucumis melo var. maku... [more]
Match NameE-valueIdentityDescription
AT3G20840.11.4e-1140.43Integrase-type DNA-binding superfamily protein [more]
AT1G51190.12.9e-0936.88Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR32467:SF143AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR PLT2coord: 56..135
NoneNo IPR availablePANTHERPTHR32467AP2-LIKE ETHYLENE-RESPONSIVE TRANSCRIPTION FACTORcoord: 56..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02726-RACarg02726-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity