Carg02506 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02506
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein DETOXIFICATION
LocationCarg_Chr02: 8404265 .. 8405794 (-)
RNA-Seq ExpressionCarg02506
SyntenyCarg02506
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCTTCTTCTGTTCCTCTACTTTCCGACCAACCAAATAGTCCGACAACTAATCATTCACTTACACCTCCTCTGATCTCCGAAAACCCAACCATTTCAACGACAGTAGCAGACAAATATCCATTTCTCTGTCTTCTTCTCTCGGAGTGCAAATCTATAGCCCACGTCGCTCTTCCGATGATTTTTGTCGGATTTTTTATGTACTCTCGTTCCATGATTTCCATGTTGTTCCTCGGCCAGTTGGGTGACTTGTCCTTGGCCGGCGGTTCCCTGGCAATTGGGTTTGCTAATATCACTGGCTACTCTGTTCTGTCTGGATTGGCCAGTGGCATGGAGCCCATCTGCGGCCAAGCTTTTGGGGCTAAAAAATTCAAACTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTTCTCCTTTCTTCGATACCCATTTCGTTTTTGTGGCTCAATATGAAGAAAATTCTGATCTTTTGTGGACAAGAGGATGATATTGCCAATGAAGCTCATTCCTTCATCCTCTGTTCTATCCCTGATTTAATTGCTCTGTCTTTTCTTCACCCTTTACGAATTTACCTTCGTTGTCAATCAATTAACCTCCCTCTCACTTACTGCGTTGGATTGTCAGTTCTTTTTCACATTCCAATTAATTACCTTCTCGTCTCTGTTCTCGATTTGGGAATTCACGGCGTCGCTTTGGGAGCTGTGTGGACGAACTTCAACCTCGTCGGATCGGTGATTATTTTCATCCTCGTCTTCGGCGTGTACAAGAATTCCTGGCCGGGATTCTCGTCGGAGTGTTTGAAGGAGTGGAAGTCGCTTCTTAGGTTAGCAATTCCAAGCTGTGTTTCGGTTTGTTTGGAATGGTGGTGGTACGAAATCATGATTATGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCCATGGGGATTTTGATTCAAACCACAGCTTTAATCTACATTTTCCCATCGTCACTAAGCGTTGGAGTTTCAACAAGAGTTGGAAACGAATTGGGAGCAAACCAACCAAACAGAGCCAAATTAGCCGCCATTGTTGGGCTCTGTTTCAGCTTCTTCTCTGGCCTTTCAGCATTGGTATTCGCTTTCAAAGTTCGAAAAGTGTGGGCCACAATGTTCACAAATGACACAGAAATCATAGAATTGACATCGCTTGTCCTCCCAATCATCGGACTTTGCGAGCTGGGGAACTGCCCACAGACGACAACCTGCGGGGTTTTGAGAGGGACAGCAAGGCCAACATTGGGAGCGAATATAAATTTGGTGTGTTTTTACATGGTGGGAATGCCGATTGCGATTTGGTTGAGCTTCTACGGTGGGTGGGACTTCAAAGGACTGTGGATTGGGCTGCTGGCGGCGCAGGCCTCATGTGCAATGGCGATGCTCATGGCGCTGGCTCGGACCAATTGGGAAGAACAGGCCGAGAGGGCTCAGGAGTTGACTAGAACCGGTCCGTTGGAGATTGAAAAATGGATAGAAAGGATGTCGAAACAAAAATGA

mRNA sequence

ATGTCTGCTTCTTCTGTTCCTCTACTTTCCGACCAACCAAATAGTCCGACAACTAATCATTCACTTACACCTCCTCTGATCTCCGAAAACCCAACCATTTCAACGACAGTAGCAGACAAATATCCATTTCTCTGTCTTCTTCTCTCGGAGTGCAAATCTATAGCCCACGTCGCTCTTCCGATGATTTTTGTCGGATTTTTTATGTACTCTCGTTCCATGATTTCCATGTTGTTCCTCGGCCAGTTGGGTGACTTGTCCTTGGCCGGCGGTTCCCTGGCAATTGGGTTTGCTAATATCACTGGCTACTCTGTTCTGTCTGGATTGGCCAGTGGCATGGAGCCCATCTGCGGCCAAGCTTTTGGGGCTAAAAAATTCAAACTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTTCTCCTTTCTTCGATACCCATTTCGTTTTTGTGGCTCAATATGAAGAAAATTCTGATCTTTTGTGGACAAGAGGATGATATTGCCAATGAAGCTCATTCCTTCATCCTCTGTTCTATCCCTGATTTAATTGCTCTGTCTTTTCTTCACCCTTTACGAATTTACCTTCGTTGTCAATCAATTAACCTCCCTCTCACTTACTGCGTTGGATTGTCAGTTCTTTTTCACATTCCAATTAATTACCTTCTCGTCTCTGTTCTCGATTTGGGAATTCACGGCGTCGCTTTGGGAGCTGTGTGGACGAACTTCAACCTCGTCGGATCGGTGATTATTTTCATCCTCGTCTTCGGCGTGTACAAGAATTCCTGGCCGGGATTCTCGTCGGAGTGTTTGAAGGAGTGGAAGTCGCTTCTTAGGTTAGCAATTCCAAGCTGTGTTTCGGTTTGTTTGGAATGGTGGTGGTACGAAATCATGATTATGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCCATGGGGATTTTGATTCAAACCACAGCTTTAATCTACATTTTCCCATCGTCACTAAGCGTTGGAGTTTCAACAAGAGTTGGAAACGAATTGGGAGCAAACCAACCAAACAGAGCCAAATTAGCCGCCATTGTTGGGCTCTGTTTCAGCTTCTTCTCTGGCCTTTCAGCATTGGTATTCGCTTTCAAAGTTCGAAAAGTGTGGGCCACAATGTTCACAAATGACACAGAAATCATAGAATTGACATCGCTTGTCCTCCCAATCATCGGACTTTGCGAGCTGGGGAACTGCCCACAGACGACAACCTGCGGGGTTTTGAGAGGGACAGCAAGGCCAACATTGGGAGCGAATATAAATTTGGTGTGTTTTTACATGGTGGGAATGCCGATTGCGATTTGGTTGAGCTTCTACGGTGGGTGGGACTTCAAAGGACTGTGGATTGGGCTGCTGGCGGCGCAGGCCTCATGTGCAATGGCGATGCTCATGGCGCTGGCTCGGACCAATTGGGAAGAACAGGCCGAGAGGGCTCAGGAGTTGACTAGAACCGGTCCGTTGGAGATTGAAAAATGGATAGAAAGGATGTCGAAACAAAAATGA

Coding sequence (CDS)

ATGTCTGCTTCTTCTGTTCCTCTACTTTCCGACCAACCAAATAGTCCGACAACTAATCATTCACTTACACCTCCTCTGATCTCCGAAAACCCAACCATTTCAACGACAGTAGCAGACAAATATCCATTTCTCTGTCTTCTTCTCTCGGAGTGCAAATCTATAGCCCACGTCGCTCTTCCGATGATTTTTGTCGGATTTTTTATGTACTCTCGTTCCATGATTTCCATGTTGTTCCTCGGCCAGTTGGGTGACTTGTCCTTGGCCGGCGGTTCCCTGGCAATTGGGTTTGCTAATATCACTGGCTACTCTGTTCTGTCTGGATTGGCCAGTGGCATGGAGCCCATCTGCGGCCAAGCTTTTGGGGCTAAAAAATTCAAACTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTTCTCCTTTCTTCGATACCCATTTCGTTTTTGTGGCTCAATATGAAGAAAATTCTGATCTTTTGTGGACAAGAGGATGATATTGCCAATGAAGCTCATTCCTTCATCCTCTGTTCTATCCCTGATTTAATTGCTCTGTCTTTTCTTCACCCTTTACGAATTTACCTTCGTTGTCAATCAATTAACCTCCCTCTCACTTACTGCGTTGGATTGTCAGTTCTTTTTCACATTCCAATTAATTACCTTCTCGTCTCTGTTCTCGATTTGGGAATTCACGGCGTCGCTTTGGGAGCTGTGTGGACGAACTTCAACCTCGTCGGATCGGTGATTATTTTCATCCTCGTCTTCGGCGTGTACAAGAATTCCTGGCCGGGATTCTCGTCGGAGTGTTTGAAGGAGTGGAAGTCGCTTCTTAGGTTAGCAATTCCAAGCTGTGTTTCGGTTTGTTTGGAATGGTGGTGGTACGAAATCATGATTATGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCCATGGGGATTTTGATTCAAACCACAGCTTTAATCTACATTTTCCCATCGTCACTAAGCGTTGGAGTTTCAACAAGAGTTGGAAACGAATTGGGAGCAAACCAACCAAACAGAGCCAAATTAGCCGCCATTGTTGGGCTCTGTTTCAGCTTCTTCTCTGGCCTTTCAGCATTGGTATTCGCTTTCAAAGTTCGAAAAGTGTGGGCCACAATGTTCACAAATGACACAGAAATCATAGAATTGACATCGCTTGTCCTCCCAATCATCGGACTTTGCGAGCTGGGGAACTGCCCACAGACGACAACCTGCGGGGTTTTGAGAGGGACAGCAAGGCCAACATTGGGAGCGAATATAAATTTGGTGTGTTTTTACATGGTGGGAATGCCGATTGCGATTTGGTTGAGCTTCTACGGTGGGTGGGACTTCAAAGGACTGTGGATTGGGCTGCTGGCGGCGCAGGCCTCATGTGCAATGGCGATGCTCATGGCGCTGGCTCGGACCAATTGGGAAGAACAGGCCGAGAGGGCTCAGGAGTTGACTAGAACCGGTCCGTTGGAGATTGAAAAATGGATAGAAAGGATGTCGAAACAAAAATGA

Protein sequence

MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEEQAERAQELTRTGPLEIEKWIERMSKQK
Homology
BLAST of Carg02506 vs. NCBI nr
Match: KAG7036064.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 990.7 bits (2560), Expect = 4.6e-285
Identity = 509/509 (100.00%), Postives = 509/509 (100.00%), Query Frame = 0

Query: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60
           MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP
Sbjct: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60

Query: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120
           MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF
Sbjct: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120

Query: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180
           GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD
Sbjct: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180

Query: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240
           LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN
Sbjct: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240

Query: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300
           FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS
Sbjct: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300

Query: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360
           GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF
Sbjct: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360

Query: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420
           SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT
Sbjct: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420

Query: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480
           ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW
Sbjct: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480

Query: 481 EEQAERAQELTRTGPLEIEKWIERMSKQK 510
           EEQAERAQELTRTGPLEIEKWIERMSKQK
Sbjct: 481 EEQAERAQELTRTGPLEIEKWIERMSKQK 509

BLAST of Carg02506 vs. NCBI nr
Match: KAG6606121.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 967.6 bits (2500), Expect = 4.2e-278
Identity = 497/499 (99.60%), Postives = 498/499 (99.80%), Query Frame = 0

Query: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60
           MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP
Sbjct: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60

Query: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120
           MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF
Sbjct: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120

Query: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180
           GAK+FKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD
Sbjct: 121 GAKRFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180

Query: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240
           LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN
Sbjct: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240

Query: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300
           FNLVGSVIIFILVFGVYKNSWPGFSS CLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS
Sbjct: 241 FNLVGSVIIFILVFGVYKNSWPGFSSACLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300

Query: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360
           GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF
Sbjct: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360

Query: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420
           SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT
Sbjct: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420

Query: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480
           ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW
Sbjct: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480

Query: 481 EEQAERAQELTRTGPLEIE 500
           EEQAERAQELTRTGPLEIE
Sbjct: 481 EEQAERAQELTRTGPLEIE 499

BLAST of Carg02506 vs. NCBI nr
Match: XP_022958539.1 (protein DETOXIFICATION 49-like [Cucurbita moschata])

HSP 1 Score: 962.6 bits (2487), Expect = 1.3e-276
Identity = 494/499 (99.00%), Postives = 497/499 (99.60%), Query Frame = 0

Query: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60
           MSASSVPLLSDQPNSPTTNHSLT PLISENPTISTTVADKYPFLCLLLSECKSIAHVALP
Sbjct: 1   MSASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60

Query: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120
           MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF
Sbjct: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120

Query: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180
           GAK+FKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD
Sbjct: 121 GAKRFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180

Query: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240
           LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFH+PINYLLVSVLDLGIHGVALGAVWTN
Sbjct: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHVPINYLLVSVLDLGIHGVALGAVWTN 240

Query: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300
           FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS
Sbjct: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300

Query: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360
           GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF
Sbjct: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360

Query: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420
           SFF GLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT
Sbjct: 361 SFFFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420

Query: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480
           ARPTLGANINLVCFY+VGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW
Sbjct: 421 ARPTLGANINLVCFYIVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480

Query: 481 EEQAERAQELTRTGPLEIE 500
           EEQAERAQELTRTGPLEIE
Sbjct: 481 EEQAERAQELTRTGPLEIE 499

BLAST of Carg02506 vs. NCBI nr
Match: XP_022995790.1 (protein DETOXIFICATION 49-like [Cucurbita maxima])

HSP 1 Score: 949.9 bits (2454), Expect = 9.0e-273
Identity = 487/499 (97.60%), Postives = 492/499 (98.60%), Query Frame = 0

Query: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60
           MSASSVPLLSDQPNSPTTNHSLT PLISENPTISTTVADKYPFLCL+LSECKSIAHVALP
Sbjct: 1   MSASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLVLSECKSIAHVALP 60

Query: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120
           MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF
Sbjct: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120

Query: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180
           GAK+FKLLGLALQRTVILLLLSSIPISF WLNMKKILIFCGQEDDIANEA SFILCSIPD
Sbjct: 121 GAKRFKLLGLALQRTVILLLLSSIPISFSWLNMKKILIFCGQEDDIANEAQSFILCSIPD 180

Query: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240
           LIALSFLHPLRIYLRCQSINLPLTYCV LSVL H+PINYLLVSVLDLGIHGVALGAVWTN
Sbjct: 181 LIALSFLHPLRIYLRCQSINLPLTYCVALSVLLHVPINYLLVSVLDLGIHGVALGAVWTN 240

Query: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300
           FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSC+SVCLEWWWYEIM+MLS
Sbjct: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCISVCLEWWWYEIMVMLS 300

Query: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360
           GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVG ELGANQPNRAKLAAIVGLCF
Sbjct: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGKELGANQPNRAKLAAIVGLCF 360

Query: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420
           SFF GLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT
Sbjct: 361 SFFFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420

Query: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480
           ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW
Sbjct: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480

Query: 481 EEQAERAQELTRTGPLEIE 500
           EEQAERAQELTRTGPLEIE
Sbjct: 481 EEQAERAQELTRTGPLEIE 499

BLAST of Carg02506 vs. NCBI nr
Match: XP_022970176.1 (protein DETOXIFICATION 49-like [Cucurbita maxima])

HSP 1 Score: 793.5 bits (2048), Expect = 1.1e-225
Identity = 407/492 (82.72%), Postives = 439/492 (89.23%), Query Frame = 0

Query: 3   ASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMI 62
           + S  +LSDQP  PT   SL  PLISE         +++P LCL+L+E K IA +ALPM+
Sbjct: 6   SGSGSVLSDQPKIPTNTTSLKAPLISE--------TERFPNLCLVLTELKCIADIALPMV 65

Query: 63  FVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGA 122
            VGF MYSRSMISMLFLG+LG LSLAGGSLAIGFANITGYSVLSGLA GMEPICGQAFGA
Sbjct: 66  LVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGA 125

Query: 123 KKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLI 182
           +KFKLLGLALQRTVILLL SSIPISFLW NMKKIL+ CGQ DDIA+EAHS+ILCS+PDLI
Sbjct: 126 QKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLI 185

Query: 183 ALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFN 242
           ALSFLHPLRIYLR QSINLPLTYC GL+++FHIPINYLLVSV D GI+GVALGAVWTNFN
Sbjct: 186 ALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDWGIYGVALGAVWTNFN 245

Query: 243 LVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGF 302
           LVG +I+FI+V GVYK +WPG SSECLKEWKSLL LAIPSC+SVCLEWWWYEIMI+LSGF
Sbjct: 246 LVGLLILFIIVSGVYKKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGF 305

Query: 303 MMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSF 362
           MMNPQSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PN+AKLAAIVGLC SF
Sbjct: 306 MMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSF 365

Query: 363 FSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTAR 422
           F GLSAL FAF VRKVWA MFTNDT IIELT LVLPIIGLCELGNCPQTT+CGVLRGTAR
Sbjct: 366 FLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTAR 425

Query: 423 PTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEE 482
           P LGANINL CFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCA+AMLMAL RTNWEE
Sbjct: 426 PKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQGSCAIAMLMALVRTNWEE 485

Query: 483 QAERAQELTRTG 495
           +AERA+ELT +G
Sbjct: 486 EAERAKELTGSG 489

BLAST of Carg02506 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.1e-177
Identity = 312/444 (70.27%), Postives = 375/444 (84.46%), Query Frame = 0

Query: 48  LSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLS-LAGGSLAIGFANITGYSVLS 107
           + E KSIA ++LP+I  G  +YSRSMISMLFLG+L DLS L+GGSLA+GFANITGYS+LS
Sbjct: 30  IQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLS 89

Query: 108 GLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDI 167
           GL+ GMEPIC QAFGAK+FKLLGLALQRT +LLLL S+PIS LWLN+KKIL+F GQ+++I
Sbjct: 90  GLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEI 149

Query: 168 ANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLD 227
           +N+A  FIL S+PDLI  SFLHP+RIYLR QSI LPLTY    +VL HIPINYLLVS L 
Sbjct: 150 SNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLG 209

Query: 228 LGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSV 287
           LG+ GVALGA+WTN NL+G +II+I+  GVY+ +W GFS +C K W+SL++LAIPSCVSV
Sbjct: 210 LGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSV 269

Query: 288 CLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQ 347
           CLEWWWYEIMI+L G ++NPQ+TVASMGILIQTTALIYIFPSSLS+ VSTRVGNELGANQ
Sbjct: 270 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 329

Query: 348 PNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELG 407
           P++A++AA  GL  S   GL A+ FA  VR  WA +FT++ EI++LTS+VLPIIGLCELG
Sbjct: 330 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 389

Query: 408 NCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQAS 467
           NCPQTT CGVLRG+ARP LGANINL CFY VGMP+A+WLSF+ G+DFKGLW+GL AAQ S
Sbjct: 390 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 449

Query: 468 CAMAMLMALARTNWEEQAERAQEL 491
           C ++ML+ LART+WE +  RA+EL
Sbjct: 450 CLISMLVVLARTDWEVEVHRAKEL 473

BLAST of Carg02506 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 5.1e-154
Identity = 279/456 (61.18%), Postives = 348/456 (76.32%), Query Frame = 0

Query: 40  KYPFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANI 99
           ++P     L E K+I  ++ P    G  MYSR+MISMLFLG LG+L LAGGSL+IGFANI
Sbjct: 48  RWPSFLEGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANI 107

Query: 100 TGYSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIF 159
           TGYSV+SGL+ GMEPICGQA+GAK+ KLLGL LQRTV+LLL  S+PISF WLNM++IL++
Sbjct: 108 TGYSVISGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLW 167

Query: 160 CGQEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINY 219
           CGQ+++I++ A  F+L +IPDL  LS LHPLRIYLR Q+I LP+TY   +SVL H+P+NY
Sbjct: 168 CGQDEEISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNY 227

Query: 220 LLVSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLA 279
           LLV  L++G+ GVA+  V TN NLV  +  F+    V+ ++W   + + LK W +LL LA
Sbjct: 228 LLVVKLEMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLA 287

Query: 280 IPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVG 339
           IP+CVSVCLEWWWYE MI+L G + NP++TVASMGILIQTTAL+Y+FPSSLS+GVSTR+ 
Sbjct: 288 IPTCVSVCLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRIS 347

Query: 340 NELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPI 399
           NELGA +P +A+++ I+ L  +   GL A+VFA  VR  W  +FT D EI++LTS+ LPI
Sbjct: 348 NELGAKRPAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPI 407

Query: 400 IGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIG 459
           +GLCELGNCPQTT CGVLRG ARPTLGANINL  FY VGMP+AI   F     F GLW G
Sbjct: 408 VGLCELGNCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFG 467

Query: 460 LLAAQASCAMAMLMALARTNWEEQAERAQELTRTGP 496
           LLAAQA+CA  ML AL RT+W+ QAERA+ELT   P
Sbjct: 468 LLAAQATCASLMLCALLRTDWKVQAERAEELTSQTP 503

BLAST of Carg02506 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.9e-140
Identity = 261/482 (54.15%), Postives = 347/482 (71.99%), Query Frame = 0

Query: 23  TPPLISENPTISTTVADK------YPFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISM 82
           TP +IS    +S    +K      +P +  L SE +S+  +A P I     +Y+RS ISM
Sbjct: 3   TPNIISHTNLLSKIDLEKQNPAPIFPTITELKSEARSLFSLAFPTILAALILYARSAISM 62

Query: 83  LFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTV 142
           LFLG +G+L LAGGSLAI FANITGYSVL+GLA GM+P+C QAFGA + KLL L LQRTV
Sbjct: 63  LFLGHIGELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFGAGRPKLLSLTLQRTV 122

Query: 143 ILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRC 202
           + LL SS+ I  LWLN+ KI+I+  Q+  I++ A ++ILCSIPDL+  SFLHPLRIYLR 
Sbjct: 123 LFLLTSSVVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDLLTNSFLHPLRIYLRA 182

Query: 203 QSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGV 262
           Q I  PLT       +FHIP+N+ LVS L  G  GV++ A  +N  +V  ++  + + G+
Sbjct: 183 QGITSPLTLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNLLVVIFLVAHVWIAGL 242

Query: 263 YKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGIL 322
           ++ +W   SSEC K+W  ++ LAIPSC+ VCLEWWWYEIM +L G +++P + VASMGIL
Sbjct: 243 HQPTWTRPSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCGLLIDPSTPVASMGIL 302

Query: 323 IQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVR 382
           IQTT+L+YIFPSSL + VSTRVGNELG+N+PN+A+L+AIV + F+   GL+A  FA+ V 
Sbjct: 303 IQTTSLLYIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFAGVMGLTASAFAWGVS 362

Query: 383 KVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYM 442
            VW  +FTND  II+LT+  LPI+GLCELGNCPQT  CGV+RGTARP++ ANINL  FY+
Sbjct: 363 DVWGWIFTNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTARPSMAANINLGAFYL 422

Query: 443 VGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEEQAERAQELTRTGPL 499
           VG P+A+ L+F+  + F GLW+GLLAAQ  CA  ML  +A T+WE++A RA++LT T  +
Sbjct: 423 VGTPVAVGLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWEKEAIRARKLTCTEGV 482

BLAST of Carg02506 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 1.4e-138
Identity = 253/454 (55.73%), Postives = 337/454 (74.23%), Query Frame = 0

Query: 44  LCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYS 103
           L + L+E  SI  ++ P++  G F+Y RS +S+ FLG LGD +LAGGSLA  FANITGYS
Sbjct: 29  LSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYS 88

Query: 104 VLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQE 163
           + SGL  G+E IC QAFGA+++  +  +++R +ILLL++S+P++ LW+NM+KIL+   Q+
Sbjct: 89  LFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQD 148

Query: 164 DDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVS 223
             +A+EAH F+L S+PDL+A SFLHPLR+YLR QS  LPL+ C  ++   H+PI + LVS
Sbjct: 149 KKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVS 208

Query: 224 VLDLGIHGVALGAVWTNFNLVGSVIIFILVF--GVYKNSWPGFSSE----CLKEWKSLLR 283
            L LGI G+AL  V +NFNLV  + ++I  F   +  N     + E     ++EWK LL 
Sbjct: 209 YLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITEETCEDSVREWKKLLC 268

Query: 284 LAIPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTR 343
           LAIPSC+SVCLEWW YEIMI+L GF+++P+++VASMGILIQ T+L+YIFP SLS+GVSTR
Sbjct: 269 LAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTR 328

Query: 344 VGNELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVL 403
           VGNELG+NQP RA+ AAIVGL  S   G +A  F   VR  WA  FT+D EI++LT++ L
Sbjct: 329 VGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMAL 388

Query: 404 PIIGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLW 463
           PI+GLCELGNCPQTT CGVLRG+ARP +GANIN V FY VG+P+   L+F+ G+ FKGLW
Sbjct: 389 PIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLW 448

Query: 464 IGLLAAQASCAMAMLMALARTNWEEQAERAQELT 492
           +G+LAAQ +C + M+ A  RT+WE +AERA+ LT
Sbjct: 449 LGMLAAQITCVIGMMAATCRTDWELEAERAKVLT 482

BLAST of Carg02506 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 7.4e-137
Identity = 245/450 (54.44%), Postives = 322/450 (71.56%), Query Frame = 0

Query: 42  PFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITG 101
           P +   ++E KS+  +A P+      +Y RS +SM FLGQLGDL LA GSLAI FANITG
Sbjct: 48  PLMAEAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITG 107

Query: 102 YSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCG 161
           YSVLSGLA GMEP+C QAFGA +FKLL L L RTV+ LL+  +PIS LW N+ KI ++  
Sbjct: 108 YSVLSGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLH 167

Query: 162 QEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLL 221
           Q+ DIA  A ++++ S+PDL+  + LHP+RIYLR Q I  P+T       +FH+P N  L
Sbjct: 168 QDPDIAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFL 227

Query: 222 VSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIP 281
           VS L LG+ GVA+ +  TN  +V  ++ ++   G++  +W   + +C + W  LLRLA P
Sbjct: 228 VSYLRLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGP 287

Query: 282 SCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNE 341
           SCVSVCLEWWWYEIMI+L G ++NP+STVA+MG+LIQTT+ +Y+FPSSLS  VSTRVGNE
Sbjct: 288 SCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNE 347

Query: 342 LGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIG 401
           LGAN+P  AKL A V + F+  +G+ A  FA+ VR  W  +FT D EI++LT+  LPI+G
Sbjct: 348 LGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILG 407

Query: 402 LCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLL 461
           LCE+GNCPQT  CGV+RGTARP+  AN+NL  FY+VGMP+A+ L F+ G  F GLW+GLL
Sbjct: 408 LCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLL 467

Query: 462 AAQASCAMAMLMALARTNWEEQAERAQELT 492
           AAQ SCA  M+  +  T+WE +A++AQ LT
Sbjct: 468 AAQISCAGLMMYVVGTTDWESEAKKAQTLT 497

BLAST of Carg02506 vs. ExPASy TrEMBL
Match: A0A6J1H5E0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459742 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.5e-277
Identity = 494/499 (99.00%), Postives = 497/499 (99.60%), Query Frame = 0

Query: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60
           MSASSVPLLSDQPNSPTTNHSLT PLISENPTISTTVADKYPFLCLLLSECKSIAHVALP
Sbjct: 1   MSASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60

Query: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120
           MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF
Sbjct: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120

Query: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180
           GAK+FKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD
Sbjct: 121 GAKRFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180

Query: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240
           LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFH+PINYLLVSVLDLGIHGVALGAVWTN
Sbjct: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHVPINYLLVSVLDLGIHGVALGAVWTN 240

Query: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300
           FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS
Sbjct: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300

Query: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360
           GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF
Sbjct: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360

Query: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420
           SFF GLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT
Sbjct: 361 SFFFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420

Query: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480
           ARPTLGANINLVCFY+VGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW
Sbjct: 421 ARPTLGANINLVCFYIVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480

Query: 481 EEQAERAQELTRTGPLEIE 500
           EEQAERAQELTRTGPLEIE
Sbjct: 481 EEQAERAQELTRTGPLEIE 499

BLAST of Carg02506 vs. ExPASy TrEMBL
Match: A0A6J1K4X4 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491221 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.4e-273
Identity = 487/499 (97.60%), Postives = 492/499 (98.60%), Query Frame = 0

Query: 1   MSASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALP 60
           MSASSVPLLSDQPNSPTTNHSLT PLISENPTISTTVADKYPFLCL+LSECKSIAHVALP
Sbjct: 1   MSASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLVLSECKSIAHVALP 60

Query: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120
           MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF
Sbjct: 61  MIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAF 120

Query: 121 GAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPD 180
           GAK+FKLLGLALQRTVILLLLSSIPISF WLNMKKILIFCGQEDDIANEA SFILCSIPD
Sbjct: 121 GAKRFKLLGLALQRTVILLLLSSIPISFSWLNMKKILIFCGQEDDIANEAQSFILCSIPD 180

Query: 181 LIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTN 240
           LIALSFLHPLRIYLRCQSINLPLTYCV LSVL H+PINYLLVSVLDLGIHGVALGAVWTN
Sbjct: 181 LIALSFLHPLRIYLRCQSINLPLTYCVALSVLLHVPINYLLVSVLDLGIHGVALGAVWTN 240

Query: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLS 300
           FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSC+SVCLEWWWYEIM+MLS
Sbjct: 241 FNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCISVCLEWWWYEIMVMLS 300

Query: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCF 360
           GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVG ELGANQPNRAKLAAIVGLCF
Sbjct: 301 GFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGKELGANQPNRAKLAAIVGLCF 360

Query: 361 SFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420
           SFF GLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT
Sbjct: 361 SFFFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGT 420

Query: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480
           ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW
Sbjct: 421 ARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNW 480

Query: 481 EEQAERAQELTRTGPLEIE 500
           EEQAERAQELTRTGPLEIE
Sbjct: 481 EEQAERAQELTRTGPLEIE 499

BLAST of Carg02506 vs. ExPASy TrEMBL
Match: A0A6J1HZW9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111469149 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 5.2e-226
Identity = 407/492 (82.72%), Postives = 439/492 (89.23%), Query Frame = 0

Query: 3   ASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMI 62
           + S  +LSDQP  PT   SL  PLISE         +++P LCL+L+E K IA +ALPM+
Sbjct: 6   SGSGSVLSDQPKIPTNTTSLKAPLISE--------TERFPNLCLVLTELKCIADIALPMV 65

Query: 63  FVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGA 122
            VGF MYSRSMISMLFLG+LG LSLAGGSLAIGFANITGYSVLSGLA GMEPICGQAFGA
Sbjct: 66  LVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFGA 125

Query: 123 KKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLI 182
           +KFKLLGLALQRTVILLL SSIPISFLW NMKKIL+ CGQ DDIA+EAHS+ILCS+PDLI
Sbjct: 126 QKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDLI 185

Query: 183 ALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFN 242
           ALSFLHPLRIYLR QSINLPLTYC GL+++FHIPINYLLVSV D GI+GVALGAVWTNFN
Sbjct: 186 ALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDWGIYGVALGAVWTNFN 245

Query: 243 LVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGF 302
           LVG +I+FI+V GVYK +WPG SSECLKEWKSLL LAIPSC+SVCLEWWWYEIMI+LSGF
Sbjct: 246 LVGLLILFIIVSGVYKKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSGF 305

Query: 303 MMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSF 362
           MMNPQSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PN+AKLAAIVGLC SF
Sbjct: 306 MMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTSF 365

Query: 363 FSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTAR 422
           F GLSAL FAF VRKVWA MFTNDT IIELT LVLPIIGLCELGNCPQTT+CGVLRGTAR
Sbjct: 366 FLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTAR 425

Query: 423 PTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEE 482
           P LGANINL CFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCA+AMLMAL RTNWEE
Sbjct: 426 PKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQGSCAIAMLMALVRTNWEE 485

Query: 483 QAERAQELTRTG 495
           +AERA+ELT +G
Sbjct: 486 EAERAKELTGSG 489

BLAST of Carg02506 vs. ExPASy TrEMBL
Match: A0A6J1E3V0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430342 PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.5e-225
Identity = 407/493 (82.56%), Postives = 442/493 (89.66%), Query Frame = 0

Query: 3   ASSVPLLSDQPNSPT-TNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPM 62
           + S  +LSDQP  PT T  SL  PLISE         +++P LC++L+E K IA +ALPM
Sbjct: 2   SGSGSVLSDQPKIPTNTTTSLNAPLISE--------TERFPNLCVVLTELKCIADIALPM 61

Query: 63  IFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFG 122
           I VGF +YSRSMISMLFLG+LG LSLAGGSLAIGFANITGYSVLSGLA GMEPICGQAFG
Sbjct: 62  ILVGFLIYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFG 121

Query: 123 AKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDL 182
           AKKFKLLGLALQRTV+LLL SS+PISFLW NMKKIL+ CGQ DDIA+EAHS+ILCS+PDL
Sbjct: 122 AKKFKLLGLALQRTVVLLLFSSVPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDL 181

Query: 183 IALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNF 242
           IALSFLHPLRIYLR QSINLPLTYC GL+++FHIPINYLLVSV DLGI+GVALGAVWTNF
Sbjct: 182 IALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNF 241

Query: 243 NLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSG 302
           NLVG +I+FI+V GVY+N+WPG SSECLKEWKSLL LAIPSC+SVCLEWWWYEIMI+LSG
Sbjct: 242 NLVGLLILFIVVSGVYENTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSG 301

Query: 303 FMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFS 362
           FM+NPQSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PN+AKLAAIVGLC S
Sbjct: 302 FMINPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTS 361

Query: 363 FFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTA 422
           FF GLSAL FAF VRKVWA MFTNDT IIELT LVLPIIGLCELGNCPQTT+CGVLRGTA
Sbjct: 362 FFLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTA 421

Query: 423 RPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWE 482
           RP LGANINL CFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQ SCA+AMLMAL RTNWE
Sbjct: 422 RPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWE 481

Query: 483 EQAERAQELTRTG 495
           EQAERA+ELT +G
Sbjct: 482 EQAERAKELTGSG 486

BLAST of Carg02506 vs. ExPASy TrEMBL
Match: A0A0A0KKM4 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G155540 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 2.0e-217
Identity = 387/480 (80.62%), Postives = 425/480 (88.54%), Query Frame = 0

Query: 21  SLTPPLISENPTISTTVADKYPF-LCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFL 80
           +L  PLI E  T       K+P+    +LSE KSIA +A PMI VGF MYSRSMISMLFL
Sbjct: 12  TLKTPLIEETET-------KFPYRFSHVLSEAKSIADIAFPMILVGFLMYSRSMISMLFL 71

Query: 81  GQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILL 140
           G+LG LSLAGGSLAIGFANITGYS+LSGLA GMEPICGQAFGAK+FKLLGL LQRT+ILL
Sbjct: 72  GRLGGLSLAGGSLAIGFANITGYSLLSGLAMGMEPICGQAFGAKRFKLLGLTLQRTIILL 131

Query: 141 LLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSI 200
           L+SS+PISFLW NMKKIL+FCGQ+ DIANEAHS+ILCS+PDL+ALSFLHPLRIYLR QSI
Sbjct: 132 LISSLPISFLWFNMKKILLFCGQDVDIANEAHSYILCSLPDLVALSFLHPLRIYLRSQSI 191

Query: 201 NLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGVYKN 260
           NLPLTYC  L++LFHIPINY  V V + GI GVALGAVWTNFN VGS+++F+L  GVYK 
Sbjct: 192 NLPLTYCAILAILFHIPINYFFVCVFEWGIRGVALGAVWTNFNFVGSLVVFVLFSGVYKK 251

Query: 261 SWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQT 320
           +WPG SS+CLKEWKSLL LAIPSC+SVCLEWWWYEIMI+LSGFM+NPQSTVASMGILIQT
Sbjct: 252 TWPGMSSDCLKEWKSLLGLAIPSCISVCLEWWWYEIMILLSGFMLNPQSTVASMGILIQT 311

Query: 321 TALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVW 380
           TALIYIFPSSLS GVSTRVGNELGAN PN+AKLAAIVGLC SFF G+SAL+FAFK+RKVW
Sbjct: 312 TALIYIFPSSLSFGVSTRVGNELGANHPNKAKLAAIVGLCISFFLGISALLFAFKIRKVW 371

Query: 381 ATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGM 440
           ATMFT D +IIELTSL+LPIIGLCELGNCPQTT+CGVLRGTARP LGANINL CFYMVGM
Sbjct: 372 ATMFTEDIQIIELTSLILPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGM 431

Query: 441 PIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEEQAERAQELTRTGPLEIE 500
           P+AIWLSFYGGWDFKGLWIGLLAAQASCAM MLM L RTNWEEQAERA+ELT+ G  EIE
Sbjct: 432 PVAIWLSFYGGWDFKGLWIGLLAAQASCAMTMLMVLTRTNWEEQAERAKELTKNGLEEIE 484

BLAST of Carg02506 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 624.4 bits (1609), Expect = 8.1e-179
Identity = 312/444 (70.27%), Postives = 375/444 (84.46%), Query Frame = 0

Query: 48  LSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLS-LAGGSLAIGFANITGYSVLS 107
           + E KSIA ++LP+I  G  +YSRSMISMLFLG+L DLS L+GGSLA+GFANITGYS+LS
Sbjct: 30  IQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGYSLLS 89

Query: 108 GLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDI 167
           GL+ GMEPIC QAFGAK+FKLLGLALQRT +LLLL S+PIS LWLN+KKIL+F GQ+++I
Sbjct: 90  GLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQDEEI 149

Query: 168 ANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLD 227
           +N+A  FIL S+PDLI  SFLHP+RIYLR QSI LPLTY    +VL HIPINYLLVS L 
Sbjct: 150 SNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLVSSLG 209

Query: 228 LGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSV 287
           LG+ GVALGA+WTN NL+G +II+I+  GVY+ +W GFS +C K W+SL++LAIPSCVSV
Sbjct: 210 LGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPSCVSV 269

Query: 288 CLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQ 347
           CLEWWWYEIMI+L G ++NPQ+TVASMGILIQTTALIYIFPSSLS+ VSTRVGNELGANQ
Sbjct: 270 CLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNELGANQ 329

Query: 348 PNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELG 407
           P++A++AA  GL  S   GL A+ FA  VR  WA +FT++ EI++LTS+VLPIIGLCELG
Sbjct: 330 PDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGLCELG 389

Query: 408 NCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQAS 467
           NCPQTT CGVLRG+ARP LGANINL CFY VGMP+A+WLSF+ G+DFKGLW+GL AAQ S
Sbjct: 390 NCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFAAQGS 449

Query: 468 CAMAMLMALARTNWEEQAERAQEL 491
           C ++ML+ LART+WE +  RA+EL
Sbjct: 450 CLISMLVVLARTDWEVEVHRAKEL 473

BLAST of Carg02506 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 545.8 bits (1405), Expect = 3.6e-155
Identity = 279/456 (61.18%), Postives = 348/456 (76.32%), Query Frame = 0

Query: 40  KYPFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANI 99
           ++P     L E K+I  ++ P    G  MYSR+MISMLFLG LG+L LAGGSL+IGFANI
Sbjct: 48  RWPSFLEGLEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANI 107

Query: 100 TGYSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIF 159
           TGYSV+SGL+ GMEPICGQA+GAK+ KLLGL LQRTV+LLL  S+PISF WLNM++IL++
Sbjct: 108 TGYSVISGLSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLW 167

Query: 160 CGQEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINY 219
           CGQ+++I++ A  F+L +IPDL  LS LHPLRIYLR Q+I LP+TY   +SVL H+P+NY
Sbjct: 168 CGQDEEISSVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNY 227

Query: 220 LLVSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLA 279
           LLV  L++G+ GVA+  V TN NLV  +  F+    V+ ++W   + + LK W +LL LA
Sbjct: 228 LLVVKLEMGVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLA 287

Query: 280 IPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVG 339
           IP+CVSVCLEWWWYE MI+L G + NP++TVASMGILIQTTAL+Y+FPSSLS+GVSTR+ 
Sbjct: 288 IPTCVSVCLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRIS 347

Query: 340 NELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPI 399
           NELGA +P +A+++ I+ L  +   GL A+VFA  VR  W  +FT D EI++LTS+ LPI
Sbjct: 348 NELGAKRPAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPI 407

Query: 400 IGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIG 459
           +GLCELGNCPQTT CGVLRG ARPTLGANINL  FY VGMP+AI   F     F GLW G
Sbjct: 408 VGLCELGNCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFG 467

Query: 460 LLAAQASCAMAMLMALARTNWEEQAERAQELTRTGP 496
           LLAAQA+CA  ML AL RT+W+ QAERA+ELT   P
Sbjct: 468 LLAAQATCASLMLCALLRTDWKVQAERAEELTSQTP 503

BLAST of Carg02506 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.3e-141
Identity = 261/482 (54.15%), Postives = 347/482 (71.99%), Query Frame = 0

Query: 23  TPPLISENPTISTTVADK------YPFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISM 82
           TP +IS    +S    +K      +P +  L SE +S+  +A P I     +Y+RS ISM
Sbjct: 3   TPNIISHTNLLSKIDLEKQNPAPIFPTITELKSEARSLFSLAFPTILAALILYARSAISM 62

Query: 83  LFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTV 142
           LFLG +G+L LAGGSLAI FANITGYSVL+GLA GM+P+C QAFGA + KLL L LQRTV
Sbjct: 63  LFLGHIGELELAGGSLAIAFANITGYSVLAGLALGMDPLCSQAFGAGRPKLLSLTLQRTV 122

Query: 143 ILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRC 202
           + LL SS+ I  LWLN+ KI+I+  Q+  I++ A ++ILCSIPDL+  SFLHPLRIYLR 
Sbjct: 123 LFLLTSSVVIVALWLNLGKIMIYLHQDPSISSLAQTYILCSIPDLLTNSFLHPLRIYLRA 182

Query: 203 QSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGV 262
           Q I  PLT       +FHIP+N+ LVS L  G  GV++ A  +N  +V  ++  + + G+
Sbjct: 183 QGITSPLTLATLAGTIFHIPMNFFLVSYLGWGFMGVSMAAAASNLLVVIFLVAHVWIAGL 242

Query: 263 YKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGIL 322
           ++ +W   SSEC K+W  ++ LAIPSC+ VCLEWWWYEIM +L G +++P + VASMGIL
Sbjct: 243 HQPTWTRPSSECFKDWGPVVTLAIPSCIGVCLEWWWYEIMTVLCGLLIDPSTPVASMGIL 302

Query: 323 IQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVR 382
           IQTT+L+YIFPSSL + VSTRVGNELG+N+PN+A+L+AIV + F+   GL+A  FA+ V 
Sbjct: 303 IQTTSLLYIFPSSLGLAVSTRVGNELGSNRPNKARLSAIVAVSFAGVMGLTASAFAWGVS 362

Query: 383 KVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYM 442
            VW  +FTND  II+LT+  LPI+GLCELGNCPQT  CGV+RGTARP++ ANINL  FY+
Sbjct: 363 DVWGWIFTNDVAIIKLTAAALPILGLCELGNCPQTVGCGVVRGTARPSMAANINLGAFYL 422

Query: 443 VGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEEQAERAQELTRTGPL 499
           VG P+A+ L+F+  + F GLW+GLLAAQ  CA  ML  +A T+WE++A RA++LT T  +
Sbjct: 423 VGTPVAVGLTFWAAYGFCGLWVGLLAAQICCAAMMLYVVATTDWEKEAIRARKLTCTEGV 482

BLAST of Carg02506 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 494.6 bits (1272), Expect = 9.6e-140
Identity = 253/454 (55.73%), Postives = 337/454 (74.23%), Query Frame = 0

Query: 44  LCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYS 103
           L + L+E  SI  ++ P++  G F+Y RS +S+ FLG LGD +LAGGSLA  FANITGYS
Sbjct: 29  LSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLGGLGDATLAGGSLAAAFANITGYS 88

Query: 104 VLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQE 163
           + SGL  G+E IC QAFGA+++  +  +++R +ILLL++S+P++ LW+NM+KIL+   Q+
Sbjct: 89  LFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLLVTSLPVTLLWMNMEKILLILKQD 148

Query: 164 DDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVS 223
             +A+EAH F+L S+PDL+A SFLHPLR+YLR QS  LPL+ C  ++   H+PI + LVS
Sbjct: 149 KKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKTLPLSICTVIASFLHLPITFFLVS 208

Query: 224 VLDLGIHGVALGAVWTNFNLVGSVIIFILVF--GVYKNSWPGFSSE----CLKEWKSLLR 283
            L LGI G+AL  V +NFNLV  + ++I  F   +  N     + E     ++EWK LL 
Sbjct: 209 YLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSVNEDEKITEETCEDSVREWKKLLC 268

Query: 284 LAIPSCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTR 343
           LAIPSC+SVCLEWW YEIMI+L GF+++P+++VASMGILIQ T+L+YIFP SLS+GVSTR
Sbjct: 269 LAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMGILIQITSLVYIFPHSLSLGVSTR 328

Query: 344 VGNELGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVL 403
           VGNELG+NQP RA+ AAIVGL  S   G +A  F   VR  WA  FT+D EI++LT++ L
Sbjct: 329 VGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVSVRNTWAMFFTDDKEIMKLTAMAL 388

Query: 404 PIIGLCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLW 463
           PI+GLCELGNCPQTT CGVLRG+ARP +GANIN V FY VG+P+   L+F+ G+ FKGLW
Sbjct: 389 PIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAFYAVGIPVGAVLAFWFGFGFKGLW 448

Query: 464 IGLLAAQASCAMAMLMALARTNWEEQAERAQELT 492
           +G+LAAQ +C + M+ A  RT+WE +AERA+ LT
Sbjct: 449 LGMLAAQITCVIGMMAATCRTDWELEAERAKVLT 482

BLAST of Carg02506 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 488.8 bits (1257), Expect = 5.3e-138
Identity = 245/450 (54.44%), Postives = 322/450 (71.56%), Query Frame = 0

Query: 42  PFLCLLLSECKSIAHVALPMIFVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITG 101
           P +   ++E KS+  +A P+      +Y RS +SM FLGQLGDL LA GSLAI FANITG
Sbjct: 48  PLMAEAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITG 107

Query: 102 YSVLSGLASGMEPICGQAFGAKKFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCG 161
           YSVLSGLA GMEP+C QAFGA +FKLL L L RTV+ LL+  +PIS LW N+ KI ++  
Sbjct: 108 YSVLSGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLH 167

Query: 162 QEDDIANEAHSFILCSIPDLIALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLL 221
           Q+ DIA  A ++++ S+PDL+  + LHP+RIYLR Q I  P+T       +FH+P N  L
Sbjct: 168 QDPDIAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFL 227

Query: 222 VSVLDLGIHGVALGAVWTNFNLVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIP 281
           VS L LG+ GVA+ +  TN  +V  ++ ++   G++  +W   + +C + W  LLRLA P
Sbjct: 228 VSYLRLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGP 287

Query: 282 SCVSVCLEWWWYEIMIMLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNE 341
           SCVSVCLEWWWYEIMI+L G ++NP+STVA+MG+LIQTT+ +Y+FPSSLS  VSTRVGNE
Sbjct: 288 SCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNE 347

Query: 342 LGANQPNRAKLAAIVGLCFSFFSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIG 401
           LGAN+P  AKL A V + F+  +G+ A  FA+ VR  W  +FT D EI++LT+  LPI+G
Sbjct: 348 LGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILG 407

Query: 402 LCELGNCPQTTTCGVLRGTARPTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLL 461
           LCE+GNCPQT  CGV+RGTARP+  AN+NL  FY+VGMP+A+ L F+ G  F GLW+GLL
Sbjct: 408 LCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLL 467

Query: 462 AAQASCAMAMLMALARTNWEEQAERAQELT 492
           AAQ SCA  M+  +  T+WE +A++AQ LT
Sbjct: 468 AAQISCAGLMMYVVGTTDWESEAKKAQTLT 497

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7036064.14.6e-285100.00Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6606121.14.2e-27899.60Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022958539.11.3e-27699.00protein DETOXIFICATION 49-like [Cucurbita moschata][more]
XP_022995790.19.0e-27397.60protein DETOXIFICATION 49-like [Cucurbita maxima][more]
XP_022970176.11.1e-22582.72protein DETOXIFICATION 49-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O827521.1e-17770.27Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1[more]
Q9SLV05.1e-15461.18Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1[more]
Q4PSF41.9e-14054.15Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1[more]
Q9FJ871.4e-13855.73Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1[more]
Q9SZE27.4e-13754.44Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H5E06.5e-27799.00Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459742 PE=3 SV=1[more]
A0A6J1K4X44.4e-27397.60Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491221 PE=3 SV=1[more]
A0A6J1HZW95.2e-22682.72Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111469149 PE=3 SV=1[more]
A0A6J1E3V01.5e-22582.56Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430342 PE=3 SV=1[more]
A0A0A0KKM42.0e-21780.63Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G155540 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G23030.18.1e-17970.27MATE efflux family protein [more]
AT1G58340.13.6e-15561.18MATE efflux family protein [more]
AT5G19700.11.3e-14154.15MATE efflux family protein [more]
AT5G52050.19.6e-14055.73MATE efflux family protein [more]
AT4G29140.15.3e-13854.44MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 60..220
e-value: 3.4E-37
score: 127.6
coord: 281..443
e-value: 3.6E-34
score: 117.8
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 60..457
e-value: 1.5E-62
score: 209.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR11206:SF374PROTEIN DETOXIFICATION 49coord: 41..495
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 41..495
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 50..486
e-value: 5.66325E-174
score: 495.169

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02506-RACarg02506-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity