Carg02474 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02474
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein DETOXIFICATION
LocationCarg_Chr02: 8540240 .. 8541700 (-)
RNA-Seq ExpressionCarg02474
SyntenyCarg02474
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGTGTCTTGTTCTACCTTGGAATCAGGGCCGTTAACCTCTGCAACTACCAAATGGGTTTCCCAAGATTTCACCAACCACATCATTTCCGAGCTGAAATTACAGAGAACCATCGCTCTTCCACTCATCGTTATGAACTTAACGTGGTTCGTAAAGATAACCATAACTACGGCGTTTCTTGGCCGCCTCGGCCAGCTTCCATTAGCCGGCGGAACACTCGGTTTTACCTTCGCTAACGTCACTGGTTTCTCCGTCTTGAACGGCCTCTGCGGCGCCATGGAACCCATTTGCGGCCAAGCTTTCGGTGCCAAGAACTTCAAACTCCTACATAAGACGCTCCTCATGTCCATTTTTCTCTTACTCCTCGTCACGATTCCAATTTCATTCCTCTGGCTCAACGTCGACTCAATTTTGATCCGTTTCGGTCAAGAGAAGGACATTTCCCTCGCCGCTAAGTCCTACCTCCTCTACCTTCTCCCCGATTTGGTCGTCACTTCATTCCTCTGTCCATTAAAATCGTATCTCAGTTCGAAAACTGAAACACTCCCGATCATGATAAGCTCGTCCATGGCTCTGGCTCTTCACGTTCCCATCAACATTTTTCTGGCTAAATCCAAGGGGTTAATTGGGGTTTCAATGGCGATCTGGGTAACCGATTTCGTCGCAATGATTTCGCTCGCGGTGTACGTTTTGGTGAAAGAGACGAATCGCGAAGGTGGATGGTTCGATCAAACGGTTGGAGATTGGATTCGATTGGCTAAACTGTCAGGACCGTGCTGCTTAACCACCTGCCTCGAATGGTGGTGCTACGAGATTCTGATCCTTCTCACGGGCCGTCTTCGCAACGCCAAACAAGCCGTGGGGACCATAGCCATCGTTTTAAACTTCGACTATTTGCTTTTTGCCGTTATGCTCTCGCTAGCCACATGCACGTCCGCGCGCGTCTCAAACGAGCTGGGCGCCAATCGTGCAACGCAAGCGCGTAGATCGGCCGGGGTGTCGGTGGTGGCAAGTGTGGGGTTTGGGTTGTTGGGGGCGGCGACGATGGTGGCGGCGAGAGGGGAATGGGGGAAGATTTTCAGTAAGGATGAAGGGACTCTGAGAATGGTGAAGAAGATGCTGGTTTTGATGGCAGCGATTGAGGTAGTGAACTATCCGTTAGCGGTTTGTGGAGGGATTGTGAGGGGGATAGGGAGGCCATTGATGGGACTATGCGCGAATCTTGGAGGATTCTACGGGGTGGCGTTGCCTTTGGGATTGATCTTGGGGTTTAAAGTTGGGGCTGGGCTTGGTGGGTTGTTGATGGGGTTTTTGGTTGGGGTATTTGGGTGTTTGGGTTTGCTATTGGCGTTCGTTTGGAGGATTGATTGGGAGAAGGAGGGTTTGAAAGCGAGTGCCATGGCAAGTGGCCAAGCCACTGGGGAGGTTGTTGTGGCTGATGTTGATGTAAAAATGTGA

mRNA sequence

ATGTCTGTGTCTTGTTCTACCTTGGAATCAGGGCCGTTAACCTCTGCAACTACCAAATGGGTTTCCCAAGATTTCACCAACCACATCATTTCCGAGCTGAAATTACAGAGAACCATCGCTCTTCCACTCATCGTTATGAACTTAACGTGGTTCGTAAAGATAACCATAACTACGGCGTTTCTTGGCCGCCTCGGCCAGCTTCCATTAGCCGGCGGAACACTCGGTTTTACCTTCGCTAACGTCACTGGTTTCTCCGTCTTGAACGGCCTCTGCGGCGCCATGGAACCCATTTGCGGCCAAGCTTTCGGTGCCAAGAACTTCAAACTCCTACATAAGACGCTCCTCATGTCCATTTTTCTCTTACTCCTCGTCACGATTCCAATTTCATTCCTCTGGCTCAACGTCGACTCAATTTTGATCCGTTTCGGTCAAGAGAAGGACATTTCCCTCGCCGCTAAGTCCTACCTCCTCTACCTTCTCCCCGATTTGGTCGTCACTTCATTCCTCTGTCCATTAAAATCGTATCTCAGTTCGAAAACTGAAACACTCCCGATCATGATAAGCTCGTCCATGGCTCTGGCTCTTCACGTTCCCATCAACATTTTTCTGGCTAAATCCAAGGGGTTAATTGGGGTTTCAATGGCGATCTGGGTAACCGATTTCGTCGCAATGATTTCGCTCGCGGTGTACGTTTTGGTGAAAGAGACGAATCGCGAAGGTGGATGGTTCGATCAAACGGTTGGAGATTGGATTCGATTGGCTAAACTGTCAGGACCGTGCTGCTTAACCACCTGCCTCGAATGGTGGTGCTACGAGATTCTGATCCTTCTCACGGGCCGTCTTCGCAACGCCAAACAAGCCGTGGGGACCATAGCCATCGTTTTAAACTTCGACTATTTGCTTTTTGCCGTTATGCTCTCGCTAGCCACATGCACGTCCGCGCGCGTCTCAAACGAGCTGGGCGCCAATCGTGCAACGCAAGCGCGTAGATCGGCCGGGGTGTCGGTGGTGGCAAGTGTGGGGTTTGGGTTGTTGGGGGCGGCGACGATGGTGGCGGCGAGAGGGGAATGGGGGAAGATTTTCAGTAAGGATGAAGGGACTCTGAGAATGGTGAAGAAGATGCTGGTTTTGATGGCAGCGATTGAGGTAGTGAACTATCCGTTAGCGGTTTGTGGAGGGATTGTGAGGGGGATAGGGAGGCCATTGATGGGACTATGCGCGAATCTTGGAGGATTCTACGGGGTGGCGTTGCCTTTGGGATTGATCTTGGGGTTTAAAGTTGGGGCTGGGCTTGGTGGGTTGTTGATGGGGTTTTTGGTTGGGGTATTTGGGTGTTTGGGTTTGCTATTGGCGTTCGTTTGGAGGATTGATTGGGAGAAGGAGGGTTTGAAAGCGAGTGCCATGGCAAGTGGCCAAGCCACTGGGGAGGTTGTTGTGGCTGATGTTGATGTAAAAATGTGA

Coding sequence (CDS)

ATGTCTGTGTCTTGTTCTACCTTGGAATCAGGGCCGTTAACCTCTGCAACTACCAAATGGGTTTCCCAAGATTTCACCAACCACATCATTTCCGAGCTGAAATTACAGAGAACCATCGCTCTTCCACTCATCGTTATGAACTTAACGTGGTTCGTAAAGATAACCATAACTACGGCGTTTCTTGGCCGCCTCGGCCAGCTTCCATTAGCCGGCGGAACACTCGGTTTTACCTTCGCTAACGTCACTGGTTTCTCCGTCTTGAACGGCCTCTGCGGCGCCATGGAACCCATTTGCGGCCAAGCTTTCGGTGCCAAGAACTTCAAACTCCTACATAAGACGCTCCTCATGTCCATTTTTCTCTTACTCCTCGTCACGATTCCAATTTCATTCCTCTGGCTCAACGTCGACTCAATTTTGATCCGTTTCGGTCAAGAGAAGGACATTTCCCTCGCCGCTAAGTCCTACCTCCTCTACCTTCTCCCCGATTTGGTCGTCACTTCATTCCTCTGTCCATTAAAATCGTATCTCAGTTCGAAAACTGAAACACTCCCGATCATGATAAGCTCGTCCATGGCTCTGGCTCTTCACGTTCCCATCAACATTTTTCTGGCTAAATCCAAGGGGTTAATTGGGGTTTCAATGGCGATCTGGGTAACCGATTTCGTCGCAATGATTTCGCTCGCGGTGTACGTTTTGGTGAAAGAGACGAATCGCGAAGGTGGATGGTTCGATCAAACGGTTGGAGATTGGATTCGATTGGCTAAACTGTCAGGACCGTGCTGCTTAACCACCTGCCTCGAATGGTGGTGCTACGAGATTCTGATCCTTCTCACGGGCCGTCTTCGCAACGCCAAACAAGCCGTGGGGACCATAGCCATCGTTTTAAACTTCGACTATTTGCTTTTTGCCGTTATGCTCTCGCTAGCCACATGCACGTCCGCGCGCGTCTCAAACGAGCTGGGCGCCAATCGTGCAACGCAAGCGCGTAGATCGGCCGGGGTGTCGGTGGTGGCAAGTGTGGGGTTTGGGTTGTTGGGGGCGGCGACGATGGTGGCGGCGAGAGGGGAATGGGGGAAGATTTTCAGTAAGGATGAAGGGACTCTGAGAATGGTGAAGAAGATGCTGGTTTTGATGGCAGCGATTGAGGTAGTGAACTATCCGTTAGCGGTTTGTGGAGGGATTGTGAGGGGGATAGGGAGGCCATTGATGGGACTATGCGCGAATCTTGGAGGATTCTACGGGGTGGCGTTGCCTTTGGGATTGATCTTGGGGTTTAAAGTTGGGGCTGGGCTTGGTGGGTTGTTGATGGGGTTTTTGGTTGGGGTATTTGGGTGTTTGGGTTTGCTATTGGCGTTCGTTTGGAGGATTGATTGGGAGAAGGAGGGTTTGAAAGCGAGTGCCATGGCAAGTGGCCAAGCCACTGGGGAGGTTGTTGTGGCTGATGTTGATGTAAAAATGTGA

Protein sequence

MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREGGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVADVDVKM
Homology
BLAST of Carg02474 vs. NCBI nr
Match: XP_022957742.1 (protein DETOXIFICATION 56 [Cucurbita moschata] >KAG7036096.1 Protein DETOXIFICATION 56, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 923.7 bits (2386), Expect = 6.6e-265
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF
Sbjct: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT
Sbjct: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240
           ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG
Sbjct: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240

Query: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300
           GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL
Sbjct: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300

Query: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360
           LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI
Sbjct: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360

Query: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420
           FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG
Sbjct: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420

Query: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480
           LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA
Sbjct: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480

Query: 481 DVDVKM 487
           DVDVKM
Sbjct: 481 DVDVKM 486

BLAST of Carg02474 vs. NCBI nr
Match: KAG6606151.1 (Protein DETOXIFICATION 56, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 915.2 bits (2364), Expect = 2.3e-262
Identity = 483/486 (99.38%), Postives = 484/486 (99.59%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           MSVS STLESGPLTSATTKWVSQDFTN IISELKLQRTIALPLIVMNLTWFVKITITTAF
Sbjct: 1   MSVSSSTLESGPLTSATTKWVSQDFTNDIISELKLQRTIALPLIVMNLTWFVKITITTAF 60

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT
Sbjct: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240
           ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG
Sbjct: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240

Query: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300
           GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL
Sbjct: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300

Query: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360
           LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGL+GAATMVAARGEWGKI
Sbjct: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLVGAATMVAARGEWGKI 360

Query: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420
           FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG
Sbjct: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420

Query: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480
           LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA
Sbjct: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480

Query: 481 DVDVKM 487
           DVDVKM
Sbjct: 481 DVDVKM 486

BLAST of Carg02474 vs. NCBI nr
Match: XP_023522427.1 (protein DETOXIFICATION 56-like [Cucurbita pepo subsp. pepo] >XP_023532882.1 protein DETOXIFICATION 56-like [Cucurbita pepo subsp. pepo] >XP_023532883.1 protein DETOXIFICATION 56-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.1 bits (2330), Expect = 2.1e-258
Identity = 477/486 (98.15%), Postives = 479/486 (98.56%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           MSVS STLESG LTSATTKWVSQDFTN IISELKLQRTIALPLIVMNLTWFVKITITTAF
Sbjct: 1   MSVSSSTLESGRLTSATTKWVSQDFTNDIISELKLQRTIALPLIVMNLTWFVKITITTAF 60

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL TIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT
Sbjct: 121 LLLATIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240
           ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG
Sbjct: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240

Query: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300
           GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLNFDYL
Sbjct: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYL 300

Query: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360
           LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGL+GAATMVAARGEWGKI
Sbjct: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLVGAATMVAARGEWGKI 360

Query: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420
           FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG
Sbjct: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420

Query: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480
           LILGFKVGAGLGGLL+GFLVGVFGCLGLLLAFVWRIDWEKEGLKASA ASGQATGEVVV 
Sbjct: 421 LILGFKVGAGLGGLLVGFLVGVFGCLGLLLAFVWRIDWEKEGLKASATASGQATGEVVVG 480

Query: 481 DVDVKM 487
           DVDVKM
Sbjct: 481 DVDVKM 486

BLAST of Carg02474 vs. NCBI nr
Match: XP_022996298.1 (protein DETOXIFICATION 56 [Cucurbita maxima])

HSP 1 Score: 890.2 bits (2299), Expect = 8.1e-255
Identity = 473/487 (97.13%), Postives = 476/487 (97.74%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           MSVS STLESGPLTSATTK +SQDFTN IISELKLQRTIALPLI MNLTWFVKITITTAF
Sbjct: 1   MSVSSSTLESGPLTSATTKRISQDFTNDIISELKLQRTIALPLIAMNLTWFVKITITTAF 60

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL TIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT
Sbjct: 121 LLLATIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240
           ETLPIMISSSMALALH+PINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKE NREG
Sbjct: 181 ETLPIMISSSMALALHIPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKERNREG 240

Query: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300
           GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLNFDYL
Sbjct: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYL 300

Query: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360
           LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGL+GAATMVAARGEWGKI
Sbjct: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLVGAATMVAARGEWGKI 360

Query: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420
           FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG
Sbjct: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420

Query: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGE-VVV 480
           LILGFKVGAGLGGLLMGFLVGVFGCLG LL FVWRIDWEKEGLKASAMASGQA GE VVV
Sbjct: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGSLLVFVWRIDWEKEGLKASAMASGQAIGEVVVV 480

Query: 481 ADVDVKM 487
           ADVDVKM
Sbjct: 481 ADVDVKM 487

BLAST of Carg02474 vs. NCBI nr
Match: XP_038875396.1 (protein DETOXIFICATION 56 [Benincasa hispida])

HSP 1 Score: 736.9 bits (1901), Expect = 1.1e-208
Identity = 387/485 (79.79%), Postives = 429/485 (88.45%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           +SVS STLESG  +   TKWVS+DFTN I+SELKLQR IALPLI MNLTWFVKI ITTAF
Sbjct: 38  VSVSSSTLESGS-SPRITKWVSKDFTNSILSELKLQRGIALPLIAMNLTWFVKIAITTAF 97

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLG LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 98  LGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLLMSIFL 157

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL T+PISFLWLNVDSILI FGQ+KDISLAAK+YLLYLLPDLV+TSFLCPLKSYLSS+T
Sbjct: 158 LLLATLPISFLWLNVDSILIHFGQQKDISLAAKTYLLYLLPDLVITSFLCPLKSYLSSQT 217

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKET---- 240
           ETLPIM+SS++ALALHVP+NIFLAKSKGL GVSMAIW+TDFVAMISLA+YV +K++    
Sbjct: 218 ETLPIMLSSALALALHVPVNIFLAKSKGLTGVSMAIWLTDFVAMISLAIYVWLKQSRSND 277

Query: 241 NREGGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLN 300
             EGGWFDQTV DW+RL KLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLN
Sbjct: 278 EEEGGWFDQTVQDWVRLVKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLN 337

Query: 301 FDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGE 360
           FDYLL++VMLSLATC SARVSNELG NR  +AR SAGVSVV SV FGL+GAA MVA RGE
Sbjct: 338 FDYLLYSVMLSLATCASARVSNELGGNRGAEARWSAGVSVVGSVVFGLMGAAAMVAGRGE 397

Query: 361 WGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVA 420
           WG+IF++DE  +RMV+KMLVLMAAIEVVN+P+AVCGG+VRG+G+PLMGL A++GGFYGVA
Sbjct: 398 WGRIFTRDEEVVRMVRKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASVGGFYGVA 457

Query: 421 LPLGLILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGE 480
           LPLG++LGFKVG GL GLL+GFLVG+FGCL LL+ FV RIDW KE  +A  M      GE
Sbjct: 458 LPLGVVLGFKVGVGLSGLLIGFLVGMFGCLVLLMVFVGRIDWGKEAQRAQLMVK---DGE 517

Query: 481 VVVAD 482
           + V D
Sbjct: 518 IAVVD 518

BLAST of Carg02474 vs. ExPASy Swiss-Prot
Match: O49660 (Protein DETOXIFICATION 56 OS=Arabidopsis thaliana OX=3702 GN=DTX56 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.4e-145
Identity = 271/453 (59.82%), Postives = 337/453 (74.39%), Query Frame = 0

Query: 22  SQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANV 81
           S+     I+ ELKLQ  I LPL+VMNL WF K+T T+ FLGR G+L LAGG+LGF+FANV
Sbjct: 20  SKTLMQSIVHELKLQMRIGLPLVVMNLLWFGKMTTTSVFLGRQGELNLAGGSLGFSFANV 79

Query: 82  TGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIR 141
           TGFSVL G+  AMEPICGQAFGAKNFKLLHKTL M++ LLLL+++PISFLWLNV  IL  
Sbjct: 80  TGFSVLYGISAAMEPICGQAFGAKNFKLLHKTLFMAVLLLLLISVPISFLWLNVHKILTG 139

Query: 142 FGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINI 201
           FGQ +DIS  AK YLLYLLP+L + SFLCPLK+YLSS+  TLPIM +++ A +LH+PINI
Sbjct: 140 FGQREDISFIAKKYLLYLLPELPILSFLCPLKAYLSSQGVTLPIMFTTAAATSLHIPINI 199

Query: 202 FLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNRE-----GGWFDQTVGDWIRLAKL 261
            L+K++G+ GV+MA+W+TDF+ +I L  YV+V E  +E     GGW +Q+  DW+ L KL
Sbjct: 200 VLSKARGIEGVAMAVWITDFIVVILLTGYVIVVERMKENKWKQGGWLNQSAQDWLTLIKL 259

Query: 262 SGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARV 321
           SGPCCLT CLEWWCYEIL+LLTGRL N  QAV  + IV NFDYLL+AVMLSL TC + RV
Sbjct: 260 SGPCCLTVCLEWWCYEILVLLTGRLPNPVQAVSILIIVFNFDYLLYAVMLSLGTCVATRV 319

Query: 322 SNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFS-KDEGTLRMVKKML 381
           SNELGAN    A R+A  +++  +  G +GA  M+A RG WG +++  D+  L  VKKM+
Sbjct: 320 SNELGANNPKGAYRAAYTTLIVGIISGCIGALVMIAFRGFWGSLYTHHDQLILNGVKKMM 379

Query: 382 VLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLL 441
           ++MA IEVVN+PL VCG IVRG  +P +G+ ANL GFY +ALPLG  L FK   GL G L
Sbjct: 380 LIMAVIEVVNFPLMVCGEIVRGTAKPSLGMYANLSGFYLLALPLGATLAFKAKQGLQGFL 439

Query: 442 MGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAM 469
           +G  VG+  CL +LL F+ RIDWEKE  KA  +
Sbjct: 440 IGLFVGISLCLSILLIFIARIDWEKEAGKAQIL 472

BLAST of Carg02474 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.2e-75
Identity = 170/458 (37.12%), Postives = 253/458 (55.24%), Query Frame = 0

Query: 30  ISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLNG 89
           ++E K   T+A P+ V  L  +++  ++  FLG+LG L LA G+L   FAN+TG+SVL+G
Sbjct: 54  VTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSG 113

Query: 90  LCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDIS 149
           L   MEP+C QAFGA  FKLL  TL  ++  LL+  +PIS LW NV  I +   Q+ DI+
Sbjct: 114 LALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIA 173

Query: 150 LAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLAK--SK 209
             A++YL++ LPDL+  + L P++ YL ++    P+ ++S      H+P N+FL      
Sbjct: 174 KLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRL 233

Query: 210 GLIGVSMAIWVTDFVAMISLAVYVLVKETNREGGWFDQT---VGDWIRLAKLSGPCCLTT 269
           GL GV++A  +T+   +  L  YV     +    W D T      W  L +L+GP C++ 
Sbjct: 234 GLTGVAVASSITNIFVVAFLVCYVWASGLHAP-TWTDPTRDCFRGWAPLLRLAGPSCVSV 293

Query: 270 CLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARVSNELGANR 329
           CLEWW YEI+I+L G L N +  V  + +++     L+    SL+   S RV NELGANR
Sbjct: 294 CLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGANR 353

Query: 330 ATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRMVKKMLVLMAAIEVV 389
              A+ +A V++V +   G++ AA   + R  WG+IF+ D+  L++    L ++   E+ 
Sbjct: 354 PKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEIG 413

Query: 390 NYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLLMGFLVGVFG 449
           N P  V  G+VRG  RP      NLG FY V +P+ + LGF  G G  GL +G L     
Sbjct: 414 NCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQIS 473

Query: 450 CLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVADV 483
           C GL++  V   DWE E  KA  +   +     ++  V
Sbjct: 474 CAGLMMYVVGTTDWESEAKKAQTLTCAETVENDIIKAV 510

BLAST of Carg02474 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.5e-73
Identity = 165/446 (37.00%), Postives = 257/446 (57.62%), Query Frame = 0

Query: 30  ISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLNG 89
           + E+K    I+ P  +  L  + +  I+  FLG LG+L LAGG+L   FAN+TG+SV++G
Sbjct: 56  LEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISG 115

Query: 90  LCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDIS 149
           L   MEPICGQA+GAK  KLL  TL  ++ LLL  ++PISF WLN+  IL+  GQ+++IS
Sbjct: 116 LSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEIS 175

Query: 150 LAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLA--KSK 209
             A+ +LL+ +PDL + S L PL+ YL ++  TLP+  S+++++ LHVP+N  L      
Sbjct: 176 SVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEM 235

Query: 210 GLIGVSMAIWVTDFVAMISLAVYVLVKETNREGGWFDQTVGD---WIRLAKLSGPCCLTT 269
           G+ GV++A+ +T+   ++ L+ +V     + +  W   T+     W  L  L+ P C++ 
Sbjct: 236 GVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSD-TWVPITIDSLKGWSALLSLAIPTCVSV 295

Query: 270 CLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARVSNELGANR 329
           CLEWW YE +I+L G L N +  V ++ I++    L++    SL+   S R+SNELGA R
Sbjct: 296 CLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKR 355

Query: 330 ATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRMVKKMLVLMAAIEVV 389
             +AR S  +S+  ++  GL+     V  R  WG++F+ D   L++    L ++   E+ 
Sbjct: 356 PAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELG 415

Query: 390 NYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLLMGFLVGVFG 449
           N P     G++RG  RP +G   NLG FY V +P+ ++ GF    G  GL  G L     
Sbjct: 416 NCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQAT 475

Query: 450 CLGLLLAFVWRIDWEKEGLKASAMAS 471
           C  L+L  + R DW+ +  +A  + S
Sbjct: 476 CASLMLCALLRTDWKVQAERAEELTS 500

BLAST of Carg02474 vs. ExPASy Swiss-Prot
Match: Q9LE20 (Protein DETOXIFICATION 54 OS=Arabidopsis thaliana OX=3702 GN=DTX54 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.9e-73
Identity = 180/478 (37.66%), Postives = 262/478 (54.81%), Query Frame = 0

Query: 22  SQDFTNH-------IISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTL 81
           S DFT+H       +I ELK    + LP+  MN   +V+  ++  FLGRLG L LAGG L
Sbjct: 7   SDDFTSHKNPTLPQVIEELKELWAMVLPITAMNCLVYVRAVVSVLFLGRLGSLELAGGAL 66

Query: 82  GFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLN 141
              F N+TG+SV+ GL   +EP+C QA+G+KN+ LL  +L   + +LL+ ++PIS LW+N
Sbjct: 67  SIGFTNITGYSVMVGLASGLEPVCSQAYGSKNWDLLTLSLHRMVVILLMASLPISLLWIN 126

Query: 142 VDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALA 201
           +  I++  GQ  +I+  A  Y LY LPDL+  + L PL+ YL S+  T P+M  +  A+A
Sbjct: 127 LGPIMLFMGQNPEITATAAEYCLYALPDLLTNTLLQPLRVYLRSQRVTKPMMWCTLAAVA 186

Query: 202 LHVPINIFLAKSK--GLIGVSMAIWVTDFVAMISLAVYV-----LVKETNREGGWFDQT- 261
            HVP+N +L   K  G+ GV++A  VT+ + ++ L  YV     L K  + +G     T 
Sbjct: 187 FHVPLNYWLVMVKHWGVPGVAIASVVTNLIMVVLLVGYVWVSGMLQKRVSGDGDGGSTTM 246

Query: 262 -------------VGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAI 321
                        VG    L +++ P CL  CLEWW YEI+I++ G L N K AV    I
Sbjct: 247 VAVVAQSSSVMELVGGLGPLMRVAVPSCLGICLEWWWYEIVIVMGGYLENPKLAVAATGI 306

Query: 322 VLNFDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAA 381
           ++    L++ V ++LA C SARV NELGA R  +AR +A V++  +   G L  A  V  
Sbjct: 307 LIQTTSLMYTVPMALAGCVSARVGNELGAGRPYKARLAANVALACAFVVGALNVAWTVIL 366

Query: 382 RGEWGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFY 441
           +  W  +F+  E    +V  ++ ++   E+ N P     GI+RG GRP +G   NLG FY
Sbjct: 367 KERWAGLFTGYEPLKVLVASVMPIVGLCELGNCPQTTGCGILRGTGRPAVGAHVNLGSFY 426

Query: 442 GVALPLGLILGFKVGAGLGGLLMGFLVGVFGC-LGLLLAFVWRIDWEKEGLKASAMAS 471
            V  P+ + L F +  G  GL  G L     C + +L A + R DWE E +KA  + S
Sbjct: 427 FVGTPVAVGLAFWLKIGFSGLWFGLLSAQAACVVSILYAVLARTDWEGEAVKAMRLTS 484

BLAST of Carg02474 vs. ExPASy Swiss-Prot
Match: Q9FH21 (Protein DETOXIFICATION 55 OS=Arabidopsis thaliana OX=3702 GN=DTX55 PE=2 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.5e-73
Identity = 169/455 (37.14%), Postives = 250/455 (54.95%), Query Frame = 0

Query: 29  IISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLN 88
           ++ ELK    I+ P+  M++  ++K   +   +GRLG L LAGG L   F N+TG+SVL+
Sbjct: 23  VVEELKRIWDISFPVAAMSILNYLKNMTSVVCMGRLGSLELAGGALAIGFTNITGYSVLS 82

Query: 89  GLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDI 148
           GL   MEP+CGQA G+KN  L   TL  +IFLLLL ++PIS LWLN+  +++   Q+ DI
Sbjct: 83  GLATGMEPLCGQAIGSKNPSLASLTLKRTIFLLLLASLPISLLWLNLAPLMLMLRQQHDI 142

Query: 149 SLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLA--KS 208
           +  A  Y  + LPDL+  SFL PL+ YL  K  T P+M  + +++ LH+PI  F     S
Sbjct: 143 TRVASLYCSFSLPDLLANSFLHPLRIYLRCKGTTWPLMWCTLVSVLLHLPITAFFTFYIS 202

Query: 209 KGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG---------------GWFDQTVGD-W 268
            G+ GV+++ ++T+F+++  L  Y+ ++  N +                G  D    D W
Sbjct: 203 LGVPGVAVSSFLTNFISLSLLLCYIYLENNNNDKTTSKSLCLDTPLMLYGSRDSGENDVW 262

Query: 269 IRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLAT 328
             L K + P C+  CLEWW YE + +L G L   K A+   AIV+    L++ +  +L+ 
Sbjct: 263 STLVKFAVPSCIAVCLEWWWYEFMTVLAGYLPEPKVALAAAAIVIQTTSLMYTIPTALSA 322

Query: 329 CTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRM 388
             S RVSNELGA R  +A+ +A V+V A+V   + G       R  WGK+F+ D+  L +
Sbjct: 323 AVSTRVSNELGAGRPEKAKTAATVAVGAAVAVSVFGLVGTTVGREAWGKVFTADKVVLEL 382

Query: 389 VKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAG 448
              ++ ++ A E+ N P  +  GI+RG  RP +G   N   FY V  P+ ++L F  G G
Sbjct: 383 TAAVIPVIGACELANCPQTISCGILRGSARPGIGAKINFYAFYVVGAPVAVVLAFVWGLG 442

Query: 449 LGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKA 466
             GL  G L     C   +L  V+  DW KE LKA
Sbjct: 443 FMGLCYGLLGAQLACAISILTVVYNTDWNKESLKA 477

BLAST of Carg02474 vs. ExPASy TrEMBL
Match: A0A6J1GZZ2 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459195 PE=3 SV=1)

HSP 1 Score: 923.7 bits (2386), Expect = 3.2e-265
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF
Sbjct: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT
Sbjct: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240
           ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG
Sbjct: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240

Query: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300
           GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL
Sbjct: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300

Query: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360
           LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI
Sbjct: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360

Query: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420
           FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG
Sbjct: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420

Query: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480
           LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA
Sbjct: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVA 480

Query: 481 DVDVKM 487
           DVDVKM
Sbjct: 481 DVDVKM 486

BLAST of Carg02474 vs. ExPASy TrEMBL
Match: A0A6J1KAE5 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491567 PE=3 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 3.9e-255
Identity = 473/487 (97.13%), Postives = 476/487 (97.74%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           MSVS STLESGPLTSATTK +SQDFTN IISELKLQRTIALPLI MNLTWFVKITITTAF
Sbjct: 1   MSVSSSTLESGPLTSATTKRISQDFTNDIISELKLQRTIALPLIAMNLTWFVKITITTAF 60

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL
Sbjct: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL TIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT
Sbjct: 121 LLLATIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG 240
           ETLPIMISSSMALALH+PINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKE NREG
Sbjct: 181 ETLPIMISSSMALALHIPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKERNREG 240

Query: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYL 300
           GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLNFDYL
Sbjct: 241 GWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLNFDYL 300

Query: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKI 360
           LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGL+GAATMVAARGEWGKI
Sbjct: 301 LFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLVGAATMVAARGEWGKI 360

Query: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420
           FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG
Sbjct: 361 FSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLG 420

Query: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGE-VVV 480
           LILGFKVGAGLGGLLMGFLVGVFGCLG LL FVWRIDWEKEGLKASAMASGQA GE VVV
Sbjct: 421 LILGFKVGAGLGGLLMGFLVGVFGCLGSLLVFVWRIDWEKEGLKASAMASGQAIGEVVVV 480

Query: 481 ADVDVKM 487
           ADVDVKM
Sbjct: 481 ADVDVKM 487

BLAST of Carg02474 vs. ExPASy TrEMBL
Match: A0A0A0KMR7 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G157920 PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 5.2e-207
Identity = 385/485 (79.38%), Postives = 429/485 (88.45%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           ++VS STLESG  +   TKWVS+DF N IISELKLQR IALPL+ MNLTWFVKI ITTAF
Sbjct: 3   VTVSSSTLESGS-SPRITKWVSKDFINSIISELKLQRGIALPLVAMNLTWFVKIAITTAF 62

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLG LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNF+LLHKTL MSIFL
Sbjct: 63  LGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFQLLHKTLFMSIFL 122

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL T+PISFLWLNVD+ILI FGQ+KD+S+AAK+YL YLLPDL++TSFLCPLKSYLSS+T
Sbjct: 123 LLLATLPISFLWLNVDTILIHFGQQKDLSIAAKTYLFYLLPDLLITSFLCPLKSYLSSQT 182

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKET---N 240
           ETLPIM+SS++ALALHVPIN+FLAKSKGLIGVS+AIWVTDFVAMISLA+YV +K++   N
Sbjct: 183 ETLPIMLSSALALALHVPINLFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQSMSNN 242

Query: 241 RE-GGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLN 300
            E GGWFDQTV DW+RL KLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLN
Sbjct: 243 EEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLN 302

Query: 301 FDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGE 360
           FDYLL++VMLSLATC SARVSNELG N    AR SAGVSVV SV  GL+GAA MVA RGE
Sbjct: 303 FDYLLYSVMLSLATCASARVSNELGRNSELAARWSAGVSVVGSVVVGLMGAAAMVAGRGE 362

Query: 361 WGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVA 420
           WG+IF++DEG +RMVKKMLVLMAAIEVVN+P+AVCGG+VRG+G+PLMGL A+LGGFYGVA
Sbjct: 363 WGRIFTRDEGVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYGVA 422

Query: 421 LPLGLILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGE 480
           LPLG++LGFKVG GLGGLL+GFLVGVF CL LL+ FVWRIDW KE  +A  MA     GE
Sbjct: 423 LPLGMVLGFKVGVGLGGLLIGFLVGVFVCLILLMVFVWRIDWGKEAQRAQLMAK---DGE 482

Query: 481 VVVAD 482
           +VV D
Sbjct: 483 IVVVD 483

BLAST of Carg02474 vs. ExPASy TrEMBL
Match: A0A5A7TN93 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003480 PE=3 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.6e-206
Identity = 387/485 (79.79%), Postives = 426/485 (87.84%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           ++VS STLESG  +   TKWVS+DF N I+SELKLQR IALPLI MNLTWFVKI ITTAF
Sbjct: 3   VTVSSSTLESGS-SPRITKWVSKDFINSILSELKLQRGIALPLIAMNLTWFVKIAITTAF 62

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLG LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTL MSIFL
Sbjct: 63  LGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLFMSIFL 122

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL T+PISFLWLNVD+ILI FGQ+KDIS+AAK+YL YLLPDL++TSFLCPLKSYLSS+T
Sbjct: 123 LLLATLPISFLWLNVDTILIHFGQQKDISIAAKTYLFYLLPDLLITSFLCPLKSYLSSQT 182

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKET---N 240
           ETLP M+SS++ALALHVPINIFLAKSKGLIGVS+AIWVTDFVAMISLA+YV +K++   N
Sbjct: 183 ETLPTMLSSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQSMSNN 242

Query: 241 RE-GGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLN 300
            E GGWFDQTV DW+RL KLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLN
Sbjct: 243 EEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLN 302

Query: 301 FDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGE 360
           FDYLL++VMLSLATC SARVSNELG N    AR SAGVSVV SV  GL+GAA MVA RGE
Sbjct: 303 FDYLLYSVMLSLATCASARVSNELGRNSGLAARWSAGVSVVGSVVVGLMGAAAMVAGRGE 362

Query: 361 WGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVA 420
           WG+IF++DE  +RMVKKMLVLMAAIEVVN+P+AVCGG+VRG+G+PLMGL A+LGGFYGVA
Sbjct: 363 WGRIFTRDEEVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYGVA 422

Query: 421 LPLGLILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGE 480
           LPLG++LGFKVG GLGGLL+GFLVGVF CL LL+ FVWRIDW KE   A  MA     GE
Sbjct: 423 LPLGMVLGFKVGVGLGGLLIGFLVGVFVCLVLLMVFVWRIDWGKEAQAAQLMAK---DGE 482

Query: 481 VVVAD 482
           VVV D
Sbjct: 483 VVVVD 483

BLAST of Carg02474 vs. ExPASy TrEMBL
Match: A0A1S3ATW5 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103482671 PE=3 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 3.4e-206
Identity = 387/485 (79.79%), Postives = 426/485 (87.84%), Query Frame = 0

Query: 1   MSVSCSTLESGPLTSATTKWVSQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAF 60
           ++VS STLESG  +   TKWVS+DF N I+SELKLQR IALPLI MNLTWFVKI ITTAF
Sbjct: 3   VTVSSSTLESGS-SPRITKWVSKDFINSILSELKLQRGIALPLIAMNLTWFVKIAITTAF 62

Query: 61  LGRLGQLPLAGGTLGFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFL 120
           LGRLG LPLA GTLGFTFANVTGFSVLNGLC AMEPICGQAFGAKNFKLLHKTL MSIFL
Sbjct: 63  LGRLGDLPLAAGTLGFTFANVTGFSVLNGLCCAMEPICGQAFGAKNFKLLHKTLFMSIFL 122

Query: 121 LLLVTIPISFLWLNVDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKT 180
           LLL T+PISFLWLNVD+ILI FGQ+KDIS+AAK+YL YLLPDL++TSFLCPLKSYLSS+T
Sbjct: 123 LLLATLPISFLWLNVDTILIHFGQQKDISIAAKTYLFYLLPDLLITSFLCPLKSYLSSQT 182

Query: 181 ETLPIMISSSMALALHVPINIFLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKET---N 240
           ETLP M+SS++ALALHVPINIFLAKSKGLIGVS+AIWVTDFVAMISLA+YV +K++   N
Sbjct: 183 ETLPTMLSSALALALHVPINIFLAKSKGLIGVSIAIWVTDFVAMISLAIYVWLKQSMSNN 242

Query: 241 RE-GGWFDQTVGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLN 300
            E GGWFDQTV DW+RL KLSGPCCLTTCLEWWCYEILILLTGRL NAKQAVGTIAIVLN
Sbjct: 243 EEGGGWFDQTVQDWVRLLKLSGPCCLTTCLEWWCYEILILLTGRLPNAKQAVGTIAIVLN 302

Query: 301 FDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGE 360
           FDYLL++VMLSLATC SARVSNELG N    AR SAGVSVV SV  GL+GAA MVA RGE
Sbjct: 303 FDYLLYSVMLSLATCASARVSNELGRNSGLAARWSAGVSVVGSVVVGLMGAAAMVAGRGE 362

Query: 361 WGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVA 420
           WG+IF++DE  +RMVKKMLVLMAAIEVVN+P+AVCGG+VRG+G+PLMGL A+LGGFYGVA
Sbjct: 363 WGRIFTRDEEVVRMVKKMLVLMAAIEVVNFPVAVCGGVVRGVGKPLMGLGASLGGFYGVA 422

Query: 421 LPLGLILGFKVGAGLGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAMASGQATGE 480
           LPLG++LGFKVG GLGGLL+GFLVGVF CL LL+ FVWRIDW KE   A  MA     GE
Sbjct: 423 LPLGVVLGFKVGVGLGGLLIGFLVGVFVCLVLLMVFVWRIDWGKEAQAAQLMAK---DGE 482

Query: 481 VVVAD 482
           VVV D
Sbjct: 483 VVVVD 483

BLAST of Carg02474 vs. TAIR 10
Match: AT4G22790.1 (MATE efflux family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.0e-146
Identity = 271/453 (59.82%), Postives = 337/453 (74.39%), Query Frame = 0

Query: 22  SQDFTNHIISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANV 81
           S+     I+ ELKLQ  I LPL+VMNL WF K+T T+ FLGR G+L LAGG+LGF+FANV
Sbjct: 20  SKTLMQSIVHELKLQMRIGLPLVVMNLLWFGKMTTTSVFLGRQGELNLAGGSLGFSFANV 79

Query: 82  TGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIR 141
           TGFSVL G+  AMEPICGQAFGAKNFKLLHKTL M++ LLLL+++PISFLWLNV  IL  
Sbjct: 80  TGFSVLYGISAAMEPICGQAFGAKNFKLLHKTLFMAVLLLLLISVPISFLWLNVHKILTG 139

Query: 142 FGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINI 201
           FGQ +DIS  AK YLLYLLP+L + SFLCPLK+YLSS+  TLPIM +++ A +LH+PINI
Sbjct: 140 FGQREDISFIAKKYLLYLLPELPILSFLCPLKAYLSSQGVTLPIMFTTAAATSLHIPINI 199

Query: 202 FLAKSKGLIGVSMAIWVTDFVAMISLAVYVLVKETNRE-----GGWFDQTVGDWIRLAKL 261
            L+K++G+ GV+MA+W+TDF+ +I L  YV+V E  +E     GGW +Q+  DW+ L KL
Sbjct: 200 VLSKARGIEGVAMAVWITDFIVVILLTGYVIVVERMKENKWKQGGWLNQSAQDWLTLIKL 259

Query: 262 SGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARV 321
           SGPCCLT CLEWWCYEIL+LLTGRL N  QAV  + IV NFDYLL+AVMLSL TC + RV
Sbjct: 260 SGPCCLTVCLEWWCYEILVLLTGRLPNPVQAVSILIIVFNFDYLLYAVMLSLGTCVATRV 319

Query: 322 SNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFS-KDEGTLRMVKKML 381
           SNELGAN    A R+A  +++  +  G +GA  M+A RG WG +++  D+  L  VKKM+
Sbjct: 320 SNELGANNPKGAYRAAYTTLIVGIISGCIGALVMIAFRGFWGSLYTHHDQLILNGVKKMM 379

Query: 382 VLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLL 441
           ++MA IEVVN+PL VCG IVRG  +P +G+ ANL GFY +ALPLG  L FK   GL G L
Sbjct: 380 LIMAVIEVVNFPLMVCGEIVRGTAKPSLGMYANLSGFYLLALPLGATLAFKAKQGLQGFL 439

Query: 442 MGFLVGVFGCLGLLLAFVWRIDWEKEGLKASAM 469
           +G  VG+  CL +LL F+ RIDWEKE  KA  +
Sbjct: 440 IGLFVGISLCLSILLIFIARIDWEKEAGKAQIL 472

BLAST of Carg02474 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 285.4 bits (729), Expect = 8.5e-77
Identity = 170/458 (37.12%), Postives = 253/458 (55.24%), Query Frame = 0

Query: 30  ISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLNG 89
           ++E K   T+A P+ V  L  +++  ++  FLG+LG L LA G+L   FAN+TG+SVL+G
Sbjct: 54  VTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITGYSVLSG 113

Query: 90  LCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDIS 149
           L   MEP+C QAFGA  FKLL  TL  ++  LL+  +PIS LW NV  I +   Q+ DI+
Sbjct: 114 LALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLHQDPDIA 173

Query: 150 LAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLAK--SK 209
             A++YL++ LPDL+  + L P++ YL ++    P+ ++S      H+P N+FL      
Sbjct: 174 KLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFLVSYLRL 233

Query: 210 GLIGVSMAIWVTDFVAMISLAVYVLVKETNREGGWFDQT---VGDWIRLAKLSGPCCLTT 269
           GL GV++A  +T+   +  L  YV     +    W D T      W  L +L+GP C++ 
Sbjct: 234 GLTGVAVASSITNIFVVAFLVCYVWASGLHAP-TWTDPTRDCFRGWAPLLRLAGPSCVSV 293

Query: 270 CLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARVSNELGANR 329
           CLEWW YEI+I+L G L N +  V  + +++     L+    SL+   S RV NELGANR
Sbjct: 294 CLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNELGANR 353

Query: 330 ATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRMVKKMLVLMAAIEVV 389
              A+ +A V++V +   G++ AA   + R  WG+IF+ D+  L++    L ++   E+ 
Sbjct: 354 PKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILGLCEIG 413

Query: 390 NYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLLMGFLVGVFG 449
           N P  V  G+VRG  RP      NLG FY V +P+ + LGF  G G  GL +G L     
Sbjct: 414 NCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLLAAQIS 473

Query: 450 CLGLLLAFVWRIDWEKEGLKASAMASGQATGEVVVADV 483
           C GL++  V   DWE E  KA  +   +     ++  V
Sbjct: 474 CAGLMMYVVGTTDWESEAKKAQTLTCAETVENDIIKAV 510

BLAST of Carg02474 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.0e-74
Identity = 165/446 (37.00%), Postives = 257/446 (57.62%), Query Frame = 0

Query: 30  ISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLNG 89
           + E+K    I+ P  +  L  + +  I+  FLG LG+L LAGG+L   FAN+TG+SV++G
Sbjct: 56  LEEVKAIGKISGPTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISG 115

Query: 90  LCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDIS 149
           L   MEPICGQA+GAK  KLL  TL  ++ LLL  ++PISF WLN+  IL+  GQ+++IS
Sbjct: 116 LSMGMEPICGQAYGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEIS 175

Query: 150 LAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLA--KSK 209
             A+ +LL+ +PDL + S L PL+ YL ++  TLP+  S+++++ LHVP+N  L      
Sbjct: 176 SVAQQFLLFAIPDLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEM 235

Query: 210 GLIGVSMAIWVTDFVAMISLAVYVLVKETNREGGWFDQTVGD---WIRLAKLSGPCCLTT 269
           G+ GV++A+ +T+   ++ L+ +V     + +  W   T+     W  L  L+ P C++ 
Sbjct: 236 GVAGVAIAMVLTNLNLVVLLSSFVYFTSVHSD-TWVPITIDSLKGWSALLSLAIPTCVSV 295

Query: 270 CLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLATCTSARVSNELGANR 329
           CLEWW YE +I+L G L N +  V ++ I++    L++    SL+   S R+SNELGA R
Sbjct: 296 CLEWWWYEFMIILCGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKR 355

Query: 330 ATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRMVKKMLVLMAAIEVV 389
             +AR S  +S+  ++  GL+     V  R  WG++F+ D   L++    L ++   E+ 
Sbjct: 356 PAKARVSMIISLFCAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELG 415

Query: 390 NYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAGLGGLLMGFLVGVFG 449
           N P     G++RG  RP +G   NLG FY V +P+ ++ GF    G  GL  G L     
Sbjct: 416 NCPQTTGCGVLRGCARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQAT 475

Query: 450 CLGLLLAFVWRIDWEKEGLKASAMAS 471
           C  L+L  + R DW+ +  +A  + S
Sbjct: 476 CASLMLCALLRTDWKVQAERAEELTS 500

BLAST of Carg02474 vs. TAIR 10
Match: AT1G71870.1 (MATE efflux family protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.4e-74
Identity = 180/478 (37.66%), Postives = 262/478 (54.81%), Query Frame = 0

Query: 22  SQDFTNH-------IISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTL 81
           S DFT+H       +I ELK    + LP+  MN   +V+  ++  FLGRLG L LAGG L
Sbjct: 7   SDDFTSHKNPTLPQVIEELKELWAMVLPITAMNCLVYVRAVVSVLFLGRLGSLELAGGAL 66

Query: 82  GFTFANVTGFSVLNGLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLN 141
              F N+TG+SV+ GL   +EP+C QA+G+KN+ LL  +L   + +LL+ ++PIS LW+N
Sbjct: 67  SIGFTNITGYSVMVGLASGLEPVCSQAYGSKNWDLLTLSLHRMVVILLMASLPISLLWIN 126

Query: 142 VDSILIRFGQEKDISLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALA 201
           +  I++  GQ  +I+  A  Y LY LPDL+  + L PL+ YL S+  T P+M  +  A+A
Sbjct: 127 LGPIMLFMGQNPEITATAAEYCLYALPDLLTNTLLQPLRVYLRSQRVTKPMMWCTLAAVA 186

Query: 202 LHVPINIFLAKSK--GLIGVSMAIWVTDFVAMISLAVYV-----LVKETNREGGWFDQT- 261
            HVP+N +L   K  G+ GV++A  VT+ + ++ L  YV     L K  + +G     T 
Sbjct: 187 FHVPLNYWLVMVKHWGVPGVAIASVVTNLIMVVLLVGYVWVSGMLQKRVSGDGDGGSTTM 246

Query: 262 -------------VGDWIRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAI 321
                        VG    L +++ P CL  CLEWW YEI+I++ G L N K AV    I
Sbjct: 247 VAVVAQSSSVMELVGGLGPLMRVAVPSCLGICLEWWWYEIVIVMGGYLENPKLAVAATGI 306

Query: 322 VLNFDYLLFAVMLSLATCTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAA 381
           ++    L++ V ++LA C SARV NELGA R  +AR +A V++  +   G L  A  V  
Sbjct: 307 LIQTTSLMYTVPMALAGCVSARVGNELGAGRPYKARLAANVALACAFVVGALNVAWTVIL 366

Query: 382 RGEWGKIFSKDEGTLRMVKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFY 441
           +  W  +F+  E    +V  ++ ++   E+ N P     GI+RG GRP +G   NLG FY
Sbjct: 367 KERWAGLFTGYEPLKVLVASVMPIVGLCELGNCPQTTGCGILRGTGRPAVGAHVNLGSFY 426

Query: 442 GVALPLGLILGFKVGAGLGGLLMGFLVGVFGC-LGLLLAFVWRIDWEKEGLKASAMAS 471
            V  P+ + L F +  G  GL  G L     C + +L A + R DWE E +KA  + S
Sbjct: 427 FVGTPVAVGLAFWLKIGFSGLWFGLLSAQAACVVSILYAVLARTDWEGEAVKAMRLTS 484

BLAST of Carg02474 vs. TAIR 10
Match: AT5G49130.1 (MATE efflux family protein )

HSP 1 Score: 276.6 bits (706), Expect = 3.9e-74
Identity = 169/455 (37.14%), Postives = 250/455 (54.95%), Query Frame = 0

Query: 29  IISELKLQRTIALPLIVMNLTWFVKITITTAFLGRLGQLPLAGGTLGFTFANVTGFSVLN 88
           ++ ELK    I+ P+  M++  ++K   +   +GRLG L LAGG L   F N+TG+SVL+
Sbjct: 23  VVEELKRIWDISFPVAAMSILNYLKNMTSVVCMGRLGSLELAGGALAIGFTNITGYSVLS 82

Query: 89  GLCGAMEPICGQAFGAKNFKLLHKTLLMSIFLLLLVTIPISFLWLNVDSILIRFGQEKDI 148
           GL   MEP+CGQA G+KN  L   TL  +IFLLLL ++PIS LWLN+  +++   Q+ DI
Sbjct: 83  GLATGMEPLCGQAIGSKNPSLASLTLKRTIFLLLLASLPISLLWLNLAPLMLMLRQQHDI 142

Query: 149 SLAAKSYLLYLLPDLVVTSFLCPLKSYLSSKTETLPIMISSSMALALHVPINIFLA--KS 208
           +  A  Y  + LPDL+  SFL PL+ YL  K  T P+M  + +++ LH+PI  F     S
Sbjct: 143 TRVASLYCSFSLPDLLANSFLHPLRIYLRCKGTTWPLMWCTLVSVLLHLPITAFFTFYIS 202

Query: 209 KGLIGVSMAIWVTDFVAMISLAVYVLVKETNREG---------------GWFDQTVGD-W 268
            G+ GV+++ ++T+F+++  L  Y+ ++  N +                G  D    D W
Sbjct: 203 LGVPGVAVSSFLTNFISLSLLLCYIYLENNNNDKTTSKSLCLDTPLMLYGSRDSGENDVW 262

Query: 269 IRLAKLSGPCCLTTCLEWWCYEILILLTGRLRNAKQAVGTIAIVLNFDYLLFAVMLSLAT 328
             L K + P C+  CLEWW YE + +L G L   K A+   AIV+    L++ +  +L+ 
Sbjct: 263 STLVKFAVPSCIAVCLEWWWYEFMTVLAGYLPEPKVALAAAAIVIQTTSLMYTIPTALSA 322

Query: 329 CTSARVSNELGANRATQARRSAGVSVVASVGFGLLGAATMVAARGEWGKIFSKDEGTLRM 388
             S RVSNELGA R  +A+ +A V+V A+V   + G       R  WGK+F+ D+  L +
Sbjct: 323 AVSTRVSNELGAGRPEKAKTAATVAVGAAVAVSVFGLVGTTVGREAWGKVFTADKVVLEL 382

Query: 389 VKKMLVLMAAIEVVNYPLAVCGGIVRGIGRPLMGLCANLGGFYGVALPLGLILGFKVGAG 448
              ++ ++ A E+ N P  +  GI+RG  RP +G   N   FY V  P+ ++L F  G G
Sbjct: 383 TAAVIPVIGACELANCPQTISCGILRGSARPGIGAKINFYAFYVVGAPVAVVLAFVWGLG 442

Query: 449 LGGLLMGFLVGVFGCLGLLLAFVWRIDWEKEGLKA 466
             GL  G L     C   +L  V+  DW KE LKA
Sbjct: 443 FMGLCYGLLGAQLACAISILTVVYNTDWNKESLKA 477

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022957742.16.6e-265100.00protein DETOXIFICATION 56 [Cucurbita moschata] >KAG7036096.1 Protein DETOXIFICAT... [more]
KAG6606151.12.3e-26299.38Protein DETOXIFICATION 56, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023522427.12.1e-25898.15protein DETOXIFICATION 56-like [Cucurbita pepo subsp. pepo] >XP_023532882.1 prot... [more]
XP_022996298.18.1e-25597.13protein DETOXIFICATION 56 [Cucurbita maxima][more]
XP_038875396.11.1e-20879.79protein DETOXIFICATION 56 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O496601.4e-14559.82Protein DETOXIFICATION 56 OS=Arabidopsis thaliana OX=3702 GN=DTX56 PE=1 SV=1[more]
Q9SZE21.2e-7537.12Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1[more]
Q9SLV01.5e-7337.00Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1[more]
Q9LE201.9e-7337.66Protein DETOXIFICATION 54 OS=Arabidopsis thaliana OX=3702 GN=DTX54 PE=2 SV=1[more]
Q9FH215.5e-7337.14Protein DETOXIFICATION 55 OS=Arabidopsis thaliana OX=3702 GN=DTX55 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GZZ23.2e-265100.00Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459195 PE=3 SV=1[more]
A0A6J1KAE53.9e-25597.13Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491567 PE=3 SV=1[more]
A0A0A0KMR75.2e-20779.38Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G157920 PE=3 SV=1[more]
A0A5A7TN932.6e-20679.79Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold4... [more]
A0A1S3ATW53.4e-20679.79Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103482671 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G22790.11.0e-14659.82MATE efflux family protein [more]
AT4G29140.18.5e-7737.12MATE efflux family protein [more]
AT1G58340.11.0e-7437.00MATE efflux family protein [more]
AT1G71870.11.4e-7437.66MATE efflux family protein [more]
AT5G49130.13.9e-7437.14MATE efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002528Multi antimicrobial extrusion proteinPFAMPF01554MatEcoord: 42..201
e-value: 4.2E-31
score: 107.8
coord: 259..421
e-value: 4.7E-29
score: 101.1
IPR002528Multi antimicrobial extrusion proteinTIGRFAMTIGR00797TIGR00797coord: 42..434
e-value: 9.9E-50
score: 167.5
NoneNo IPR availablePANTHERPTHR11206:SF93PROTEIN DETOXIFICATION 56coord: 22..471
NoneNo IPR availablePANTHERPTHR11206MULTIDRUG RESISTANCE PROTEINcoord: 22..471
IPR045069Multidrug and toxic compound extrusion family, eukaryoticCDDcd13132MATE_eukaryoticcoord: 32..461
e-value: 4.24435E-116
score: 346.867

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02474-RACarg02474-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015297 antiporter activity
molecular_function GO:0042910 xenobiotic transmembrane transporter activity