Carg02217 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02217
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription initiation factor TFIID subunit 8-like
LocationCarg_Chr15: 1318417 .. 1319457 (+)
RNA-Seq ExpressionCarg02217
SyntenyCarg02217
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGATGGAGGTGAAGACTTCCCACGGGCGCTTGCTAGGATTGCTGTATCCCAAATATGTGAGAGTGAGGGGTTTCAAACTTTTCAACAGTCTGCCCTCGAAACACTGGCGGACGTTGCAGCTCGGTATGTTCATAACATGGGAAGGGCCGCAAACTTCTGTGCTAACTTCGCCGGCAGAACAGAGTGTAATGTATTGGATATAATGAAAGCACAGGAAGATTTGGGGTCTGTCCAAGGCTTTGCAGGTGCTTCGGATATTGAATATTGTCTCGCGAGTTCCGGCACTGCTAAAGAGATTTCTCGGTATGTAGCTCGAGCTGAAGAAATCCCATTTGCCTACTCTGTTCCCAAATTTCCATTTGTCAAGGAGCGAAAGTTGAGGCCTACTTTTTGGCAAATTGGGGAAGAACCTCCTGGAGAACATATACCTTCTTGGTTGCCTGCAATCCCTGATCCTGAAGCAGACATTGAGCCCTCCACTGTGAAGGAGGAAGTAGTGGAGCCTCAAACGATTAAAACTGAGCTAGAAAAACCATGTAAAAGTGCAGAACAATCCATCTGGAATCTGCAGAGGTGGTTGTTTTGCCATGGATTGGAAGGATACCAAAGAGAAGGCCCTAGAAATGCAGCTATGATCAAACAAACACAAGAAAGTAACCCATTTTTTGCTCCCCCACTGCCATTTGGTGAAAAGGAAATTTCTTCTTTCGTTCTTCCAGATAGTATATTGAACAATTCAAGTACAGAATATCCTGTTCCTATCATGGAGAACTGTCTTGTGGATACACATGTCTCAGTGCTGGAGACTTTTGCTCCTGCTATTGAATCAATAAAGAACAATTTGTACGATTCCGATGAAAAGTTCTCTCTGAACAGACGATCCACTATGCAGTTTAAGATTGGAACTGGAAAGAAGCCTGTCGGTAGCAGGATAGAATTGAAGGTTCCAAACAATGGTGTGAAGACGTCTTCGTCCTGGTTTGTAGCAGAAGATGAGAAGTATGACAAGAAAAGGAAGGTCGAGAAGATTCTTTAG

mRNA sequence

ATGAGCGATGGAGGTGAAGACTTCCCACGGGCGCTTGCTAGGATTGCTGTATCCCAAATATGTGAGAGTGAGGGGTTTCAAACTTTTCAACAGTCTGCCCTCGAAACACTGGCGGACGTTGCAGCTCGGTATGTTCATAACATGGGAAGGGCCGCAAACTTCTGTGCTAACTTCGCCGGCAGAACAGAGTGTAATGTATTGGATATAATGAAAGCACAGGAAGATTTGGGGTCTGTCCAAGGCTTTGCAGGTGCTTCGGATATTGAATATTGTCTCGCGAGTTCCGGCACTGCTAAAGAGATTTCTCGGTATGTAGCTCGAGCTGAAGAAATCCCATTTGCCTACTCTGTTCCCAAATTTCCATTTGTCAAGGAGCGAAAGTTGAGGCCTACTTTTTGGCAAATTGGGGAAGAACCTCCTGGAGAACATATACCTTCTTGGTTGCCTGCAATCCCTGATCCTGAAGCAGACATTGAGCCCTCCACTGTGAAGGAGGAAGTAGTGGAGCCTCAAACGATTAAAACTGAGCTAGAAAAACCATGTAAAAGTGCAGAACAATCCATCTGGAATCTGCAGAGGTGGTTGTTTTGCCATGGATTGGAAGGATACCAAAGAGAAGGCCCTAGAAATGCAGCTATGATCAAACAAACACAAGAAAGTAACCCATTTTTTGCTCCCCCACTGCCATTTGGTGAAAAGGAAATTTCTTCTTTCGTTCTTCCAGATAGTATATTGAACAATTCAAGTACAGAATATCCTGTTCCTATCATGGAGAACTGTCTTGTGGATACACATGTCTCAGTGCTGGAGACTTTTGCTCCTGCTATTGAATCAATAAAGAACAATTTGTACGATTCCGATGAAAAGTTCTCTCTGAACAGACGATCCACTATGCAGTTTAAGATTGGAACTGGAAAGAAGCCTGTCGGTAGCAGGATAGAATTGAAGGTTCCAAACAATGGTGTGAAGACGTCTTCGTCCTGGTTTGTAGCAGAAGATGAGAAGTATGACAAGAAAAGGAAGGTCGAGAAGATTCTTTAG

Coding sequence (CDS)

ATGAGCGATGGAGGTGAAGACTTCCCACGGGCGCTTGCTAGGATTGCTGTATCCCAAATATGTGAGAGTGAGGGGTTTCAAACTTTTCAACAGTCTGCCCTCGAAACACTGGCGGACGTTGCAGCTCGGTATGTTCATAACATGGGAAGGGCCGCAAACTTCTGTGCTAACTTCGCCGGCAGAACAGAGTGTAATGTATTGGATATAATGAAAGCACAGGAAGATTTGGGGTCTGTCCAAGGCTTTGCAGGTGCTTCGGATATTGAATATTGTCTCGCGAGTTCCGGCACTGCTAAAGAGATTTCTCGGTATGTAGCTCGAGCTGAAGAAATCCCATTTGCCTACTCTGTTCCCAAATTTCCATTTGTCAAGGAGCGAAAGTTGAGGCCTACTTTTTGGCAAATTGGGGAAGAACCTCCTGGAGAACATATACCTTCTTGGTTGCCTGCAATCCCTGATCCTGAAGCAGACATTGAGCCCTCCACTGTGAAGGAGGAAGTAGTGGAGCCTCAAACGATTAAAACTGAGCTAGAAAAACCATGTAAAAGTGCAGAACAATCCATCTGGAATCTGCAGAGGTGGTTGTTTTGCCATGGATTGGAAGGATACCAAAGAGAAGGCCCTAGAAATGCAGCTATGATCAAACAAACACAAGAAAGTAACCCATTTTTTGCTCCCCCACTGCCATTTGGTGAAAAGGAAATTTCTTCTTTCGTTCTTCCAGATAGTATATTGAACAATTCAAGTACAGAATATCCTGTTCCTATCATGGAGAACTGTCTTGTGGATACACATGTCTCAGTGCTGGAGACTTTTGCTCCTGCTATTGAATCAATAAAGAACAATTTGTACGATTCCGATGAAAAGTTCTCTCTGAACAGACGATCCACTATGCAGTTTAAGATTGGAACTGGAAAGAAGCCTGTCGGTAGCAGGATAGAATTGAAGGTTCCAAACAATGGTGTGAAGACGTCTTCGTCCTGGTTTGTAGCAGAAGATGAGAAGTATGACAAGAAAAGGAAGGTCGAGAAGATTCTTTAG

Protein sequence

MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL
Homology
BLAST of Carg02217 vs. NCBI nr
Match: KAG7016105.1 (Transcription initiation factor TFIID subunit 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 699.1 bits (1803), Expect = 1.9e-197
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60
           MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG
Sbjct: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60

Query: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120
           RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF
Sbjct: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120

Query: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180
           PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP
Sbjct: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180

Query: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240
           CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL
Sbjct: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240

Query: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300
           PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF
Sbjct: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300

Query: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL
Sbjct: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 346

BLAST of Carg02217 vs. NCBI nr
Match: KAG6578544.1 (Transcription initiation factor TFIID subunit 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693.7 bits (1789), Expect = 7.9e-196
Identity = 343/346 (99.13%), Postives = 344/346 (99.42%), Query Frame = 0

Query: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60
           MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVA RYVHNMGRAANFCANFAG
Sbjct: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAVRYVHNMGRAANFCANFAG 60

Query: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120
           RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLAS GTAKEISRYVARAEEIPFAYSVPKF
Sbjct: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASYGTAKEISRYVARAEEIPFAYSVPKF 120

Query: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180
           PFVKERKLRP+FWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP
Sbjct: 121 PFVKERKLRPSFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180

Query: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240
           CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL
Sbjct: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240

Query: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300
           PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF
Sbjct: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300

Query: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL
Sbjct: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 346

BLAST of Carg02217 vs. NCBI nr
Match: XP_022939031.1 (transcription initiation factor TFIID subunit 8-like [Cucurbita moschata])

HSP 1 Score: 690.3 bits (1780), Expect = 8.7e-195
Identity = 341/346 (98.55%), Postives = 343/346 (99.13%), Query Frame = 0

Query: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60
           MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVA RYVHNMGRAANFCANFAG
Sbjct: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAVRYVHNMGRAANFCANFAG 60

Query: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120
           RTECNVLD+MKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF
Sbjct: 61  RTECNVLDVMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120

Query: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180
           PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP
Sbjct: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180

Query: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240
           C+SAEQSIWNLQRWLFCHGLEG QREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL
Sbjct: 181 CRSAEQSIWNLQRWLFCHGLEGSQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240

Query: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300
           PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF
Sbjct: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300

Query: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDE YDKKRKVEKIL
Sbjct: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEMYDKKRKVEKIL 346

BLAST of Carg02217 vs. NCBI nr
Match: XP_022993704.1 (transcription initiation factor TFIID subunit 8-like [Cucurbita maxima])

HSP 1 Score: 657.9 bits (1696), Expect = 4.8e-185
Identity = 330/346 (95.38%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60
           MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVA RYVHNMGRAANFCANFAG
Sbjct: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAVRYVHNMGRAANFCANFAG 60

Query: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120
           RTE NVLDIMKA EDLGSVQGFAGASDIEYCLA SGT KEI+RYVARAEEIPFAYSVPKF
Sbjct: 61  RTEYNVLDIMKALEDLGSVQGFAGASDIEYCLARSGTVKEIARYVARAEEIPFAYSVPKF 120

Query: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180
           P VKERKLRPTF QIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP
Sbjct: 121 PVVKERKLRPTFLQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180

Query: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240
           C+SAEQSIWNLQRWLFCHGLEG QREGPRNAAMIKQTQESNPFFAPPL FGEKEISSFVL
Sbjct: 181 CRSAEQSIWNLQRWLFCHGLEGSQREGPRNAAMIKQTQESNPFFAPPLQFGEKEISSFVL 240

Query: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300
           PDSILNNSSTEY VPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEK SLNR STMQF
Sbjct: 241 PDSILNNSSTEYLVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKLSLNRLSTMQF 300

Query: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           KIGTGKKPVGSRIE KVPNNGVKTSSSWFVAEDE+YDKKRKVEKIL
Sbjct: 301 KIGTGKKPVGSRIESKVPNNGVKTSSSWFVAEDEQYDKKRKVEKIL 346

BLAST of Carg02217 vs. NCBI nr
Match: XP_023538136.1 (transcription initiation factor TFIID subunit 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 565.1 bits (1455), Expect = 4.2e-157
Identity = 277/343 (80.76%), Postives = 308/343 (89.80%), Query Frame = 0

Query: 4   GGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTE 63
           GGEDFP+ALA+IAV+QICESEGFQTFQQSALETLAD+A RYV N+G+ ANFCANFAGRTE
Sbjct: 22  GGEDFPQALAKIAVAQICESEGFQTFQQSALETLADIAVRYVQNIGKTANFCANFAGRTE 81

Query: 64  CNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFV 123
           CNV DI++A EDLGS+QGFAGA DIE+CLASS T KEI+RYVA+AEEIPFAY+VPKFP V
Sbjct: 82  CNVFDIIQALEDLGSIQGFAGALDIEHCLASSSTVKEIARYVAQAEEIPFAYAVPKFPVV 141

Query: 124 KERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKS 183
           KERKLRPTF QIGEE PG+HIPSWLPA+PDPE  I   TV+E+VVEPQ IKTE EK CKS
Sbjct: 142 KERKLRPTFLQIGEETPGKHIPSWLPALPDPETYIASPTVEEKVVEPQKIKTEPEKQCKS 201

Query: 184 AEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDS 243
           AE+S+WNLQ+WLFC+GLEG +REGPRNAAM KQTQESNPF APPL FGEKE+SS VLPD 
Sbjct: 202 AEKSLWNLQQWLFCNGLEGSRREGPRNAAMTKQTQESNPFLAPPLQFGEKEVSSVVLPDR 261

Query: 244 ILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIG 303
           ILNNSST+YPVP  ENCLVDTHVSVLETFAPAIES+KN+L+DS+EKFSLNRRST+QFKIG
Sbjct: 262 ILNNSSTQYPVPARENCLVDTHVSVLETFAPAIESMKNSLHDSEEKFSLNRRSTVQFKIG 321

Query: 304 TGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           TGKKPV S IELK  NNGVK SSSWFV +D+K DKKRK EKIL
Sbjct: 322 TGKKPVSSMIELKALNNGVKNSSSWFVGDDKKDDKKRKAEKIL 364

BLAST of Carg02217 vs. ExPASy Swiss-Prot
Match: Q9SYZ9 (Transcription initiation factor TFIID subunit 8 OS=Arabidopsis thaliana OX=3702 GN=TAF8 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 8.3e-39
Identity = 112/343 (32.65%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 7   DFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECNV 66
           +F  A A+ AV+Q+CES G++ F+  ALE+L+  A +Y+  +G+ A   AN  GR++CNV
Sbjct: 25  EFSHAAAKAAVAQVCESVGYENFKDPALESLSGFALQYILQLGKTATSFANLTGRSQCNV 84

Query: 67  LDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFV--- 126
            DI+ A +DL    G  G S     L  S   +EI  +V  +EE+PF+  +P FP     
Sbjct: 85  FDIILALDDLTDNNGEQGISSESCSLGRSIKLREIIDFVNSSEEVPFSQPLPSFPVAISD 144

Query: 127 KERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKS 186
           + RK+ P+F +IGE PPG+HIP WLPA PDP    E     E V +P+  K E  +  + 
Sbjct: 145 RSRKMIPSFVEIGETPPGKHIPLWLPAFPDPHTYKETPMWIERVSDPRGDKIEQARQRRK 204

Query: 187 AEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDS 246
           AE+++ +LQR L C         G  +   +K+    +      +  GEK  S       
Sbjct: 205 AERALLSLQRKLVCKISSRNPVWGDMDG--VKEEMRDDESELRSVSSGEKVES------- 264

Query: 247 ILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIG 306
            LN                   +SV+E FAPA+E+ ++             +     K+ 
Sbjct: 265 -LNRDG----------------LSVIEAFAPAMEAARDGFSSEAHTEWKKNKPVALSKLR 324

Query: 307 TGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           T KK +G  ++L +   G     S FV E+++ DK+R+ E IL
Sbjct: 325 TEKKFLGQPLDLSLQMKGEDRPIS-FVREEDRDDKRRRAEFIL 340

BLAST of Carg02217 vs. ExPASy Swiss-Prot
Match: Q5VWG9 (Transcription initiation factor TFIID subunit 3 OS=Homo sapiens OX=9606 GN=TAF3 PE=1 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.1e-10
Identity = 55/203 (27.09%), Postives = 92/203 (45.32%), Query Frame = 0

Query: 6   EDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECN 65
           E + R+L R++V+QIC++ G+ + Q SA   L DV  RY+  +GR  +  +   GRT+  
Sbjct: 3   ESYSRSLLRVSVAQICQALGWDSVQLSACHLLTDVLQRYLQQLGRGCHRYSELYGRTD-P 62

Query: 66  VLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFVKE 125
           +LD      D+G      G S             E+  Y+   E + F + +P FP  K 
Sbjct: 63  ILD------DVGEAFQLMGVS-----------LHELEDYIHNIEPVTFPHQIPSFPVSKN 122

Query: 126 RKLR-PTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEE-VVEPQTIKTELEKPCKS 185
             L+ P       E   E+IP +LP I   + + E   V  +     + ++  LE+  + 
Sbjct: 123 NVLQFPQPGSKDAEERKEYIPDYLPPIVSSQEEEEEEQVPTDGGTSAEAMQVPLEEDDEL 182

Query: 186 AEQSIWNLQRWLFCHGLEGYQRE 207
            E+ I N + +L    L+  + E
Sbjct: 183 EEEEIINDENFLGKRPLDSPEAE 187

BLAST of Carg02217 vs. ExPASy Swiss-Prot
Match: Q5HZG4 (Transcription initiation factor TFIID subunit 3 OS=Mus musculus OX=10090 GN=Taf3 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 8.1e-10
Identity = 48/192 (25.00%), Postives = 88/192 (45.83%), Query Frame = 0

Query: 6   EDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECN 65
           E + R+L R++V+QIC++ G+ + Q SA   L DV  RY+  +GR  +  +   GRT+  
Sbjct: 3   ESYSRSLLRVSVAQICQALGWDSVQLSACHLLTDVLQRYLQQLGRGCHRYSELYGRTDPI 62

Query: 66  VLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFVKE 125
           + D+ +A + +G                      E+  Y+   E + F + +P FP  K 
Sbjct: 63  LDDVGEAFQLMGV------------------NLHELEDYIHNIEPVTFPHQIPSFPVSKN 122

Query: 126 RKLR-PTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEE-VVEPQTIKTELEKPCKS 185
             L+ P       E   ++IP +LP I   + + E   V  +     + ++  LE+  + 
Sbjct: 123 NVLQFPQPGSKDAEERKDYIPDYLPPIVSSQEEEEEEQVPTDGGTSAEAMQVPLEEDDEM 176

Query: 186 AEQSIWNLQRWL 196
            E+ + N + +L
Sbjct: 183 EEEEVINDENFL 176

BLAST of Carg02217 vs. ExPASy Swiss-Prot
Match: Q5F489 (Transcription initiation factor TFIID subunit 3 OS=Gallus gallus OX=9031 GN=TAF3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 2.4e-09
Identity = 54/203 (26.60%), Postives = 88/203 (43.35%), Query Frame = 0

Query: 6   EDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECN 65
           E + R L R++V+QIC++ G+ + Q SA + L DV  RY+  +GR  +      GRT+  
Sbjct: 3   ETYSRWLLRVSVAQICQALGWDSVQVSACDLLTDVLQRYLQGLGRGCHRYCELYGRTD-P 62

Query: 66  VLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFVKE 125
           +LD      D+G      G +             E+  Y+   E + FA+ +P FP  K 
Sbjct: 63  ILD------DVGDAFKLMGVN-----------LHELEDYIHNIEPVTFAHQIPSFPVSKN 122

Query: 126 RKLR-PTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEE-VVEPQTIKTELEKPCKS 185
             L+ P       E   E+IP ++P I   + + E   V  +     + ++  LE+    
Sbjct: 123 NVLQFPQPGSKDAEERKEYIPDYMPPIVSSQEEEEEEQVPTDGGTSAEAMQVPLEEEGDM 182

Query: 186 AEQSIWNLQRWLFCHGLEGYQRE 207
            E    N + +L    LE    E
Sbjct: 183 EEDEAINDENYLSKRPLESPDAE 187

BLAST of Carg02217 vs. ExPASy Swiss-Prot
Match: Q28J24 (Transcription initiation factor TFIID subunit 8 OS=Xenopus tropicalis OX=8364 GN=taf8 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 9.3e-06
Identity = 42/179 (23.46%), Postives = 77/179 (43.02%), Query Frame = 0

Query: 10  RALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECNVLDI 69
           R   ++ VS +    GF++ +++A+E+L ++   Y+  +GR+A        RT+  + DI
Sbjct: 24  RRTLQVVVSSLLTEAGFESAEKAAVESLTEMLQSYLSEIGRSAKSYCEHTARTQPTLPDI 83

Query: 70  MKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFVKERKLR 129
           +    ++G         ++E   +    AK   R V  A  +     VPK     + K  
Sbjct: 84  VVTLIEMG--------FNVE---SLPAYAKRSQRMVITAPPVTNNPVVPKALSAGQNKQH 143

Query: 130 PTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKSAEQSI 189
           P            HIPS  P  PDP   I+  T +E V + Q ++ +     +  E+++
Sbjct: 144 PA-----------HIPSHFPEFPDPHTYIKTPTYREPVCDYQVLREKAASQRRDVERAL 180

BLAST of Carg02217 vs. ExPASy TrEMBL
Match: A0A6J1FES1 (transcription initiation factor TFIID subunit 8-like OS=Cucurbita moschata OX=3662 GN=LOC111445056 PE=4 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.2e-195
Identity = 341/346 (98.55%), Postives = 343/346 (99.13%), Query Frame = 0

Query: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60
           MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVA RYVHNMGRAANFCANFAG
Sbjct: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAVRYVHNMGRAANFCANFAG 60

Query: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120
           RTECNVLD+MKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF
Sbjct: 61  RTECNVLDVMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120

Query: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180
           PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP
Sbjct: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180

Query: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240
           C+SAEQSIWNLQRWLFCHGLEG QREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL
Sbjct: 181 CRSAEQSIWNLQRWLFCHGLEGSQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240

Query: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300
           PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF
Sbjct: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300

Query: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDE YDKKRKVEKIL
Sbjct: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEMYDKKRKVEKIL 346

BLAST of Carg02217 vs. ExPASy TrEMBL
Match: A0A6J1K0V4 (transcription initiation factor TFIID subunit 8-like OS=Cucurbita maxima OX=3661 GN=LOC111489615 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.3e-185
Identity = 330/346 (95.38%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAG 60
           MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVA RYVHNMGRAANFCANFAG
Sbjct: 1   MSDGGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAVRYVHNMGRAANFCANFAG 60

Query: 61  RTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKF 120
           RTE NVLDIMKA EDLGSVQGFAGASDIEYCLA SGT KEI+RYVARAEEIPFAYSVPKF
Sbjct: 61  RTEYNVLDIMKALEDLGSVQGFAGASDIEYCLARSGTVKEIARYVARAEEIPFAYSVPKF 120

Query: 121 PFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180
           P VKERKLRPTF QIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP
Sbjct: 121 PVVKERKLRPTFLQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKP 180

Query: 181 CKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVL 240
           C+SAEQSIWNLQRWLFCHGLEG QREGPRNAAMIKQTQESNPFFAPPL FGEKEISSFVL
Sbjct: 181 CRSAEQSIWNLQRWLFCHGLEGSQREGPRNAAMIKQTQESNPFFAPPLQFGEKEISSFVL 240

Query: 241 PDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQF 300
           PDSILNNSSTEY VPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEK SLNR STMQF
Sbjct: 241 PDSILNNSSTEYLVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKLSLNRLSTMQF 300

Query: 301 KIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           KIGTGKKPVGSRIE KVPNNGVKTSSSWFVAEDE+YDKKRKVEKIL
Sbjct: 301 KIGTGKKPVGSRIESKVPNNGVKTSSSWFVAEDEQYDKKRKVEKIL 346

BLAST of Carg02217 vs. ExPASy TrEMBL
Match: A0A6J1E0M7 (Transcription initiation factor TFIID subunit 8 OS=Cucurbita moschata OX=3662 GN=LOC111429744 PE=3 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 3.9e-156
Identity = 276/343 (80.47%), Postives = 305/343 (88.92%), Query Frame = 0

Query: 4   GGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTE 63
           GGEDFP+ALA+IAV+QICESEGFQTFQQSALETLAD+A RYV N+G+ ANFCANFAGRTE
Sbjct: 22  GGEDFPQALAKIAVAQICESEGFQTFQQSALETLADIAVRYVQNIGKTANFCANFAGRTE 81

Query: 64  CNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFV 123
           CNV D+++A EDLGSVQGFAGA DIE CLASS T KEI+RYVA+AEEIPFAY+VPKFP V
Sbjct: 82  CNVFDVIQALEDLGSVQGFAGALDIERCLASSSTVKEIARYVAQAEEIPFAYAVPKFPVV 141

Query: 124 KERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKS 183
           KERKLRPTF Q GEE PG+HIPSWLPA+PDPE  I   TV+E+VVEPQ IKTE EK CKS
Sbjct: 142 KERKLRPTFLQTGEETPGKHIPSWLPALPDPETYIASPTVEEKVVEPQKIKTEPEKQCKS 201

Query: 184 AEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDS 243
           AE+S+WNLQ+WLFC+GLEG +REGPRNAAM KQTQESNPF APPL FGEKE+SS VLPD 
Sbjct: 202 AEKSLWNLQQWLFCNGLEGSRREGPRNAAMTKQTQESNPFLAPPLQFGEKEVSSVVLPDR 261

Query: 244 ILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIG 303
           ILNNSSTEYPVP  ENCLVDTHVSVLETFAPAIES+KN+L+DS+EKFSLNRRST+QFKIG
Sbjct: 262 ILNNSSTEYPVPARENCLVDTHVSVLETFAPAIESLKNSLHDSEEKFSLNRRSTVQFKIG 321

Query: 304 TGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           TGKKP  S IELK  NNGVK SSSWFV +D+K DKKRK EKIL
Sbjct: 322 TGKKPESSMIELKALNNGVKNSSSWFVGDDKKDDKKRKAEKIL 364

BLAST of Carg02217 vs. ExPASy TrEMBL
Match: A0A6J1JHK2 (Transcription initiation factor TFIID subunit 8 OS=Cucurbita maxima OX=3661 GN=LOC111487069 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 8.6e-156
Identity = 276/343 (80.47%), Postives = 306/343 (89.21%), Query Frame = 0

Query: 4   GGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTE 63
           GGEDFP+ALA+IA++QICESEGFQTFQQSALETLAD+A RYV N+G+ ANFCANFAGRTE
Sbjct: 22  GGEDFPQALAKIAIAQICESEGFQTFQQSALETLADIAVRYVQNIGKTANFCANFAGRTE 81

Query: 64  CNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFV 123
           CNV DI++A EDLGSVQGFAGA DIE+CLASS T KEI+RYVA+AEEIPFAY+VPKFP V
Sbjct: 82  CNVFDIIQALEDLGSVQGFAGALDIEHCLASSSTVKEIARYVAQAEEIPFAYAVPKFPVV 141

Query: 124 KERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKS 183
           KERKLRPTF QIGEE PG+HIPSWLPA+PDPE  I   TV+E+VVEPQ IKTE EK CK 
Sbjct: 142 KERKLRPTFLQIGEETPGKHIPSWLPALPDPETYIASPTVEEKVVEPQKIKTEPEKQCKG 201

Query: 184 AEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDS 243
           AE+S+WNLQ+ LFC+GLEG +REGPRNAAM KQTQESNPF APPL FGEKE+SS VLPD 
Sbjct: 202 AEKSLWNLQQGLFCNGLEGSRREGPRNAAMTKQTQESNPFLAPPLQFGEKEVSSVVLPDR 261

Query: 244 ILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIG 303
           ILNNSSTEYPVP  ENCLVDTHVSVLETFAPAIES+KN+L+DS+EKFSLNRRST+QFKIG
Sbjct: 262 ILNNSSTEYPVPARENCLVDTHVSVLETFAPAIESMKNSLHDSEEKFSLNRRSTVQFKIG 321

Query: 304 TGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           TGKKPV S IELK  NNGVK SSSWFV +D+K DKKRK EKIL
Sbjct: 322 TGKKPVSSMIELKALNNGVKNSSSWFVGDDKKDDKKRKAEKIL 364

BLAST of Carg02217 vs. ExPASy TrEMBL
Match: A0A0A0KSJ1 (Transcription initiation factor TFIID subunit 8 OS=Cucumis sativus OX=3659 GN=Csa_5G639490 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.8e-153
Identity = 274/343 (79.88%), Postives = 300/343 (87.46%), Query Frame = 0

Query: 4   GGEDFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTE 63
           G EDFPRALA+IAV+QICESEGFQ FQQSALETLADVA RYV NMG  ANFCANFAGRTE
Sbjct: 22  GSEDFPRALAKIAVAQICESEGFQIFQQSALETLADVAVRYVQNMGSTANFCANFAGRTE 81

Query: 64  CNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFV 123
           CN+ DI++A EDLGSVQGFAGASDIE+CLASS T KE +RYVA+AEE+PFAYSVPKFP V
Sbjct: 82  CNLFDIIQALEDLGSVQGFAGASDIEHCLASSSTVKEFARYVAQAEEVPFAYSVPKFPVV 141

Query: 124 KERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKS 183
           KERKLRP+F QIGEEPPGEHIPSWLPA+PDPE  IE   VKEEVVEPQTIKTE EK C++
Sbjct: 142 KERKLRPSFLQIGEEPPGEHIPSWLPALPDPETYIESPIVKEEVVEPQTIKTEPEKQCRT 201

Query: 184 AEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDS 243
            E+S WNLQ+WLFC+GLEG QRE PRNAAM KQ QESNPF APPL FGEKE+SS VLPD 
Sbjct: 202 -EKSFWNLQQWLFCNGLEGSQREDPRNAAMTKQIQESNPFLAPPLQFGEKEVSSIVLPDK 261

Query: 244 ILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIG 303
           +LNNSSTEY VP+MENC VDTHVSVLETFAPAIESIKNN + S+EK+SLNR+ST+QFKIG
Sbjct: 262 VLNNSSTEYHVPVMENCQVDTHVSVLETFAPAIESIKNNFHMSEEKYSLNRKSTVQFKIG 321

Query: 304 TGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           TGKK  G+ IEL+  NNGVK SSSWFV EDEK DKKRK EKIL
Sbjct: 322 TGKKAAGNMIELRALNNGVKKSSSWFVGEDEKDDKKRKAEKIL 363

BLAST of Carg02217 vs. TAIR 10
Match: AT3G02160.1 (Bromodomain transcription factor )

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-51
Identity = 132/359 (36.77%), Postives = 191/359 (53.20%), Query Frame = 0

Query: 5   GEDFPRALARIAVSQICES------------EG--FQTFQQSALETLADVAARYVHNMGR 64
           G DF  ++AR+AV+QICES            EG  F +FQ+SAL+TL DVA +Y+ ++G+
Sbjct: 40  GNDFAFSIARMAVAQICESVEVNPYQESQTREGVRFSSFQESALDTLTDVAVQYIQSIGK 99

Query: 65  AANFCANFAGRTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEE 124
            A+  AN AGR + N LDI++A EDLGS  GFAG SD ++CLA SG  K+I RY   AEE
Sbjct: 100 TAHLYANLAGRVDGNSLDILQALEDLGSGLGFAGVSDTDHCLADSGVVKDIIRYTGEAEE 159

Query: 125 IPFAYSVPKFPFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEP 184
           IPF YS+P+FPF KE+K  P+F ++G EPP EHIP WLPA P+ E        + E    
Sbjct: 160 IPFVYSLPRFPFSKEKKPAPSFSEVGAEPPDEHIPVWLPAFPETEL-----CDRSEETNA 219

Query: 185 QTIKTELEKPCKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPF 244
            TI+ E+  P K    S+ ++Q      G     +         +   E N F   PL F
Sbjct: 220 ATIEGEI--PSKENGSSLPSMQLSFDGGGRLEIHKSSKDVGESTEAVVEGNLFLTAPLRF 279

Query: 245 GEKEISSFVLPDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNN---LYDSD 304
            EK +S  V P  + N       VP  ++   + H+ +LE  AP+ +    N   +    
Sbjct: 280 VEKNVSPVVRPLELSNEVVRTNHVP-DKHVRNNHHIPILEASAPSDKINNKNWLAISKDV 339

Query: 305 EKFSLNRR--STMQFKIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEK 345
           EK  + R+  + ++FKIGT K+       + +  N       WF   ++K +K  ++++
Sbjct: 340 EKVDVARKELTLVRFKIGTTKR------SMCLAKNRSFQEEGWFQEGEDKREKTSEIKE 384

BLAST of Carg02217 vs. TAIR 10
Match: AT5G15570.1 (Bromodomain transcription factor )

HSP 1 Score: 195.7 bits (496), Expect = 6.3e-50
Identity = 131/362 (36.19%), Postives = 191/362 (52.76%), Query Frame = 0

Query: 5   GEDFPRALARIAVSQICES-------------EG--FQTFQQSALETLADVAARYVHNMG 64
           G DF  ALAR+A +QICES             EG  F +FQ++ALETL DV  +Y+ N+G
Sbjct: 24  GNDFAYALARMATAQICESVEINSYQESSQSREGLRFSSFQETALETLTDVVIQYIQNIG 83

Query: 65  RAANFCANFAGRTECNVLDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAE 124
           + A F  N AGR E N LDI++A EDLGS  GF GA D+E+CLA SG  K+I RY   AE
Sbjct: 84  KTAQFYVNMAGRVESNALDIVQALEDLGSGLGFDGAHDVEHCLADSGVVKDIIRYTGEAE 143

Query: 125 EIPFAYSVPKFPFVKERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVE 184
           EIPF YS+P+FPF + ++  P+F  IG EPP EHIP WLPA P+ +     S   EE+  
Sbjct: 144 EIPFVYSLPRFPFNRGKRPAPSFSDIGVEPPDEHIPVWLPAFPETKM----SNGSEEI-- 203

Query: 185 PQTIKTELEKPCKSAEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLP 244
               K E +   +    S+ ++Q+ +    L+  +    ++     +  E NPF A P+ 
Sbjct: 204 -NVDKIERDVQSRDNGSSLMSVQQSVDVDRLKVQKSMDQKDVQKPIEEPEGNPFLAAPIW 263

Query: 245 FGEKEI--SSFVLPDSILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIE-SIKNNLYDS 304
            GEK +  S  V P  +     +   +P  ++  +  H+  LE +A + + + KN L   
Sbjct: 264 VGEKNVSLSRVVCPSELRKEEISTNHLP-EKHMSMSHHIPALEAYALSDKINDKNRLAGM 323

Query: 305 DEKFSLNRRST----MQFKIGTGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKV 345
           +++   +   T    ++FKIGT K     +I   +   G      WF  +  K +KK + 
Sbjct: 324 EDEQKRDGARTQGALLRFKIGTRKASECWKINQCLEEKG------WFKEDGNKREKKVER 371

BLAST of Carg02217 vs. TAIR 10
Match: AT4G34340.1 (TBP-associated factor 8 )

HSP 1 Score: 162.5 bits (410), Expect = 5.9e-40
Identity = 112/343 (32.65%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 7   DFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECNV 66
           +F  A A+ AV+Q+CES G++ F+  ALE+L+  A +Y+  +G+ A   AN  GR++CNV
Sbjct: 25  EFSHAAAKAAVAQVCESVGYENFKDPALESLSGFALQYILQLGKTATSFANLTGRSQCNV 84

Query: 67  LDIMKAQEDLGSVQGFAGASDIEYCLASSGTAKEISRYVARAEEIPFAYSVPKFPFV--- 126
            DI+ A +DL    G  G S     L  S   +EI  +V  +EE+PF+  +P FP     
Sbjct: 85  FDIILALDDLTDNNGEQGISSESCSLGRSIKLREIIDFVNSSEEVPFSQPLPSFPVAISD 144

Query: 127 KERKLRPTFWQIGEEPPGEHIPSWLPAIPDPEADIEPSTVKEEVVEPQTIKTELEKPCKS 186
           + RK+ P+F +IGE PPG+HIP WLPA PDP    E     E V +P+  K E  +  + 
Sbjct: 145 RSRKMIPSFVEIGETPPGKHIPLWLPAFPDPHTYKETPMWIERVSDPRGDKIEQARQRRK 204

Query: 187 AEQSIWNLQRWLFCHGLEGYQREGPRNAAMIKQTQESNPFFAPPLPFGEKEISSFVLPDS 246
           AE+++ +LQR L C         G  +   +K+    +      +  GEK  S       
Sbjct: 205 AERALLSLQRKLVCKISSRNPVWGDMDG--VKEEMRDDESELRSVSSGEKVES------- 264

Query: 247 ILNNSSTEYPVPIMENCLVDTHVSVLETFAPAIESIKNNLYDSDEKFSLNRRSTMQFKIG 306
            LN                   +SV+E FAPA+E+ ++             +     K+ 
Sbjct: 265 -LNRDG----------------LSVIEAFAPAMEAARDGFSSEAHTEWKKNKPVALSKLR 324

Query: 307 TGKKPVGSRIELKVPNNGVKTSSSWFVAEDEKYDKKRKVEKIL 347
           T KK +G  ++L +   G     S FV E+++ DK+R+ E IL
Sbjct: 325 TEKKFLGQPLDLSLQMKGEDRPIS-FVREEDRDDKRRRAEFIL 340

BLAST of Carg02217 vs. TAIR 10
Match: AT1G31240.1 (Bromodomain transcription factor )

HSP 1 Score: 77.0 bits (188), Expect = 3.3e-14
Identity = 53/159 (33.33%), Postives = 80/159 (50.31%), Query Frame = 0

Query: 7   DFPRALARIAVSQICESEGFQTFQQSALETLADVAARYVHNMGRAANFCANFAGRTECNV 66
           +F  +L +IAVSQIC+S G++    SAL TL     +++ ++   A+  +N A RTE N+
Sbjct: 25  EFSFSLTKIAVSQICQSIGYKATDASALNTLTLTTTKFLQSLAELASSFSNTANRTEVNL 84

Query: 67  LDIMKAQED--LGSVQGFAGAS---DIE-YCLASSGTAKEISRYVARAEEIPFAYSVPKF 126
            DI+   +D  L +   F G S   DIE  CL  S   + +S +V  A EIPFA  +P+ 
Sbjct: 85  FDIVNGLQDIALSTSDCFPGGSTVHDIESQCLIKSAVLRNLSDFVTYAPEIPFAKPLPR- 144

Query: 127 PFVKERKLRPTFW------QIGEEPPGEHIPSWLPAIPD 154
                R+   +F        +        +P+WLP  PD
Sbjct: 145 -----RERDGSFGGDLDHVAVTRSVDVTSVPAWLPPFPD 177

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016105.11.9e-197100.00Transcription initiation factor TFIID subunit 8, partial [Cucurbita argyrosperma... [more]
KAG6578544.17.9e-19699.13Transcription initiation factor TFIID subunit 8, partial [Cucurbita argyrosperma... [more]
XP_022939031.18.7e-19598.55transcription initiation factor TFIID subunit 8-like [Cucurbita moschata][more]
XP_022993704.14.8e-18595.38transcription initiation factor TFIID subunit 8-like [Cucurbita maxima][more]
XP_023538136.14.2e-15780.76transcription initiation factor TFIID subunit 8-like [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q9SYZ98.3e-3932.65Transcription initiation factor TFIID subunit 8 OS=Arabidopsis thaliana OX=3702 ... [more]
Q5VWG92.1e-1027.09Transcription initiation factor TFIID subunit 3 OS=Homo sapiens OX=9606 GN=TAF3 ... [more]
Q5HZG48.1e-1025.00Transcription initiation factor TFIID subunit 3 OS=Mus musculus OX=10090 GN=Taf3... [more]
Q5F4892.4e-0926.60Transcription initiation factor TFIID subunit 3 OS=Gallus gallus OX=9031 GN=TAF3... [more]
Q28J249.3e-0623.46Transcription initiation factor TFIID subunit 8 OS=Xenopus tropicalis OX=8364 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1FES14.2e-19598.55transcription initiation factor TFIID subunit 8-like OS=Cucurbita moschata OX=36... [more]
A0A6J1K0V42.3e-18595.38transcription initiation factor TFIID subunit 8-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1E0M73.9e-15680.47Transcription initiation factor TFIID subunit 8 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JHK28.6e-15680.47Transcription initiation factor TFIID subunit 8 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0KSJ11.8e-15379.88Transcription initiation factor TFIID subunit 8 OS=Cucumis sativus OX=3659 GN=Cs... [more]
Match NameE-valueIdentityDescription
AT3G02160.12.0e-5136.77Bromodomain transcription factor [more]
AT5G15570.16.3e-5036.19Bromodomain transcription factor [more]
AT4G34340.15.9e-4032.65TBP-associated factor 8 [more]
AT1G31240.13.3e-1433.33Bromodomain transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006565Bromodomain associated domainSMARTSM0057617neu3coord: 6..82
e-value: 1.4E-23
score: 94.3
IPR006565Bromodomain associated domainPFAMPF07524Bromo_TPcoord: 9..77
e-value: 1.4E-18
score: 66.7
IPR009072Histone-foldGENE3D1.10.20.10Histone, subunit Acoord: 3..89
e-value: 8.8E-21
score: 75.4
IPR009072Histone-foldSUPERFAMILY47113Histone-foldcoord: 17..74
IPR037818Transcription initiation factor TFIID subunit 8PANTHERPTHR46338TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 8coord: 4..346
NoneNo IPR availablePANTHERPTHR46338:SF7BROMODOMAIN TRANSCRIPTION FACTORcoord: 4..346

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02217-RACarg02217-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
cellular_component GO:0005669 transcription factor TFIID complex
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0003743 translation initiation factor activity