Carg02145 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02145
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionbasic helix-loop-helix (bHLH) DNA-binding superfamily protein
LocationCarg_Chr15: 893352 .. 894492 (+)
RNA-Seq ExpressionCarg02145
SyntenyCarg02145
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAATTCAAATCATGTCTTATATATATATATATATATGGTTGTAGAGGAAGCTAGCCAACACAAGTTGGTATAGATTTGAGGCCTCACCATGCCCTTATAAAAACACACACAAGAAAAAGAAACATATAACACACAAACAAAAAAGCTACAATCCATCCATGGTTTCTAGAGAGCACAACAACGCATCTCTTCATCACAACCTCCAATTACTTCGCTCTATTACCAATTCTCATGCTGTAATTTCCCTCTCTTTTCTCTTCATTTTGTTCATTGCGTTCTTCGCGTTCTTCGTGTTCTTCGTGTTCTTCGATTCGATTCTCAATTCATTCATAAAAAGTTATGATTTTTATGTGTGTAGCAGCTCAACAAGGCCTCGATTATAGTGGATGCATCAAAATACATCGAGGAGCTTAAACAAAAAGTAGAAAGATTGAATCAAGATATCTCAACCGTTCAAACTTCAATCCATCCCATGGTATTTTCTTCAATCAACAACAACTTTTTCCCTTGAATTATTCAAGAACAACCAAAAAAAAACATACCCATTTGTTCATTCTAGCAGGTGACAGTGGAAAGCCTAGCAAAGGGGTTTTCCATTAATGTATTTTCAGAAAAGAGCTGCCAAGGCCTGCTCGTGTCGGTATTAGAAGCCTTTGAAGAGCTGGGGCTGAATGTTCTTGAAGCTAGGGTTTCTTGTACAGATAGTTTCCAATTACAAGCTATTGCAGAAGTAAGATACTCCAAGCATCCATCTTTCTATAATTATGCATATAAATCATCATCATTATAAAAACATATATTTATTATTATTATTCCATGGTCAATAAAGTCAAATCAATCATTCATTTTAACAAGAATATTTACCTAAAGTAATGGAATTTTCCATCTAATCATACATTTCAATGTCGGGTTTTTTTTTTTTTTTTTTTTTAATATATGATATTCAATTTAATGTGTGGATTAAAATGGGTGTTTGTAATTTTATAGATTGAGGAACAAGGAGAAGAAGCCATTGATGCTCAAGCTGTGAAAGAAGCAGTAGTTGAAGCTATAAAGATCTGGAGCCAAAGCGGTGAACAAGATTAAAAACATCCTTAATTTCTCCAGCTTGTTCCAATTGCGGCTGTTTTTTTTTTTTT

mRNA sequence

TATAATTCAAATCATGTCTTATATATATATATATATATGGTTGTAGAGGAAGCTAGCCAACACAAGTTGGTATAGATTTGAGGCCTCACCATGCCCTTATAAAAACACACACAAGAAAAAGAAACATATAACACACAAACAAAAAAGCTACAATCCATCCATGGTTTCTAGAGAGCACAACAACGCATCTCTTCATCACAACCTCCAATTACTTCGCTCTATTACCAATTCTCATGCTCAGCTCAACAAGGCCTCGATTATAGTGGATGCATCAAAATACATCGAGGAGCTTAAACAAAAAGTAGAAAGATTGAATCAAGATATCTCAACCGTTCAAACTTCAATCCATCCCATGGTGACAGTGGAAAGCCTAGCAAAGGGGTTTTCCATTAATGTATTTTCAGAAAAGAGCTGCCAAGGCCTGCTCGTGTCGGTATTAGAAGCCTTTGAAGAGCTGGGGCTGAATGTTCTTGAAGCTAGGGTTTCTTGTACAGATAGTTTCCAATTACAAGCTATTGCAGAAATTGAGGAACAAGGAGAAGAAGCCATTGATGCTCAAGCTGTGAAAGAAGCAGTAGTTGAAGCTATAAAGATCTGGAGCCAAAGCGGTGAACAAGATTAAAAACATCCTTAATTTCTCCAGCTTGTTCCAATTGCGGCTGTTTTTTTTTTTTT

Coding sequence (CDS)

ATGGTTTCTAGAGAGCACAACAACGCATCTCTTCATCACAACCTCCAATTACTTCGCTCTATTACCAATTCTCATGCTCAGCTCAACAAGGCCTCGATTATAGTGGATGCATCAAAATACATCGAGGAGCTTAAACAAAAAGTAGAAAGATTGAATCAAGATATCTCAACCGTTCAAACTTCAATCCATCCCATGGTGACAGTGGAAAGCCTAGCAAAGGGGTTTTCCATTAATGTATTTTCAGAAAAGAGCTGCCAAGGCCTGCTCGTGTCGGTATTAGAAGCCTTTGAAGAGCTGGGGCTGAATGTTCTTGAAGCTAGGGTTTCTTGTACAGATAGTTTCCAATTACAAGCTATTGCAGAAATTGAGGAACAAGGAGAAGAAGCCATTGATGCTCAAGCTGTGAAAGAAGCAGTAGTTGAAGCTATAAAGATCTGGAGCCAAAGCGGTGAACAAGATTAA

Protein sequence

MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQTSIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIAEIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD
Homology
BLAST of Carg02145 vs. NCBI nr
Match: KAG7016033.1 (Transcription factor bHLH61 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 283.1 bits (723), Expect = 1.4e-72
Identity = 153/153 (100.00%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD
Sbjct: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 153

BLAST of Carg02145 vs. NCBI nr
Match: XP_023551078.1 (uncharacterized protein LOC111809011 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 281.6 bits (719), Expect = 4.1e-72
Identity = 151/153 (98.69%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEEQGEEAIDAQAVKEAVV+AIKIWSQSGEQD
Sbjct: 121 EIEEQGEEAIDAQAVKEAVVQAIKIWSQSGEQD 153

BLAST of Carg02145 vs. NCBI nr
Match: XP_022939239.1 (uncharacterized protein LOC111445214 isoform X3 [Cucurbita moschata])

HSP 1 Score: 280.4 bits (716), Expect = 9.2e-72
Identity = 150/153 (98.04%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEEQGEEAIDAQ+VKEAVV+AIKIWSQSGEQD
Sbjct: 121 EIEEQGEEAIDAQSVKEAVVQAIKIWSQSGEQD 153

BLAST of Carg02145 vs. NCBI nr
Match: XP_022993986.1 (uncharacterized protein LOC111489817 isoform X3 [Cucurbita maxima])

HSP 1 Score: 279.3 bits (713), Expect = 2.1e-71
Identity = 149/153 (97.39%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNA+LHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNAALHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEE+GEEAIDAQAVKEAVV+AIKIWSQSGEQD
Sbjct: 121 EIEEEGEEAIDAQAVKEAVVQAIKIWSQSGEQD 153

BLAST of Carg02145 vs. NCBI nr
Match: XP_023551076.1 (uncharacterized protein LOC111809011 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.9 bits (707), Expect = 1.0e-70
Identity = 151/154 (98.05%), Postives = 153/154 (99.35%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPM-VTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAI 120
           SIHPM VTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAI
Sbjct: 61  SIHPMQVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAI 120

Query: 121 AEIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           AEIEEQGEEAIDAQAVKEAVV+AIKIWSQSGEQD
Sbjct: 121 AEIEEQGEEAIDAQAVKEAVVQAIKIWSQSGEQD 154

BLAST of Carg02145 vs. ExPASy Swiss-Prot
Match: Q9LXA9 (Transcription factor bHLH61 OS=Arabidopsis thaliana OX=3702 GN=BHLH61 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.8e-06
Identity = 43/154 (27.92%), Postives = 78/154 (50.65%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           +++       L+  L LLRSI     ++++ SI+ DA  Y++EL  K+ +L +D   + +
Sbjct: 153 LMAERRRRKRLNDRLSLLRSIVPKITKMDRTSILGDAIDYMKELLDKINKLQEDEQELGS 212

Query: 61  SIH--PMVTVESLAKGF--------SINVFSEKSC---QGLLVSVLEAFEELGLNVLEAR 120
           + H   ++T ES+ +           +N   +  C    GL+VS +   E LGL + +  
Sbjct: 213 NSHLSTLITNESMVRNSLKFEVDQREVNTHIDICCPTKPGLVVSTVSTLETLGLEIEQCV 272

Query: 121 VSCTDSFQLQAIA-EIEEQGEEAIDAQAVKEAVV 141
           +SC   F LQA   E+ EQ    + ++A K+A++
Sbjct: 273 ISCFSDFSLQASCFEVGEQ-RYMVTSEATKQALI 305

BLAST of Carg02145 vs. ExPASy Swiss-Prot
Match: Q10S44 (Transcription factor BHLH3 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH3 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 6.5e-04
Identity = 33/160 (20.62%), Postives = 72/160 (45.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           +++       L+  L +LRSI    +++++ SI+ D   Y++EL ++++ L ++I     
Sbjct: 184 LMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYVKELTERIKTLEEEIGVTPE 243

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQ---------------------GLLVSVLEAFEEL 120
            +  + T++  + G +  +    S +                     G+L+S + A E L
Sbjct: 244 ELDLLNTMKDSSSGNNNEMLVRNSTKFDVENRGSGNTRIEICCPANPGVLLSTVSALEVL 303

Query: 121 GLNVLEARVSCTDSFQLQAIAEIEEQGEEAIDAQAVKEAV 140
           GL + +  VSC   F +QA    E+   + +    +K+ +
Sbjct: 304 GLEIEQCVVSCFSDFGMQASCLQEDGKRQVVSTDEIKQTL 343

BLAST of Carg02145 vs. ExPASy Swiss-Prot
Match: Q9LPW3 (Transcription factor SCREAM2 OS=Arabidopsis thaliana OX=3702 GN=SCRM2 PE=1 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 8.5e-04
Identity = 39/175 (22.29%), Postives = 84/175 (48.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTV-- 60
           +++       L+  L +LRS+    +++++ASI+ DA  Y++EL Q++  L+ ++ +   
Sbjct: 269 LMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHTELESTPP 328

Query: 61  -QTSIHPMV-TVESLA------------------------------KGFSINVFSEKSCQ 120
             +S+HP+  T ++L+                              K  +I++F  +   
Sbjct: 329 SSSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLREGKAVNIHMFCGRR-P 388

Query: 121 GLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIAEIEEQGEEAIDAQAVKEAVVE 142
           GLL+S + A + LGL+V +A +SC + F L      + Q +  +  + +K  +++
Sbjct: 389 GLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQCQEDHDVLPEQIKAVLLD 442

BLAST of Carg02145 vs. ExPASy TrEMBL
Match: A0A6J1FGK6 (uncharacterized protein LOC111445214 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111445214 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 4.5e-72
Identity = 150/153 (98.04%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEEQGEEAIDAQ+VKEAVV+AIKIWSQSGEQD
Sbjct: 121 EIEEQGEEAIDAQSVKEAVVQAIKIWSQSGEQD 153

BLAST of Carg02145 vs. ExPASy TrEMBL
Match: A0A6J1JXW1 (uncharacterized protein LOC111489817 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111489817 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 9.9e-72
Identity = 149/153 (97.39%), Postives = 153/153 (100.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNA+LHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNAALHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEE+GEEAIDAQAVKEAVV+AIKIWSQSGEQD
Sbjct: 121 EIEEEGEEAIDAQAVKEAVVQAIKIWSQSGEQD 153

BLAST of Carg02145 vs. ExPASy TrEMBL
Match: A0A6J1FG89 (uncharacterized protein LOC111445214 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445214 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.1e-70
Identity = 150/154 (97.40%), Postives = 153/154 (99.35%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPM-VTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAI 120
           SIHPM VTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAI
Sbjct: 61  SIHPMQVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAI 120

Query: 121 AEIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           AEIEEQGEEAIDAQ+VKEAVV+AIKIWSQSGEQD
Sbjct: 121 AEIEEQGEEAIDAQSVKEAVVQAIKIWSQSGEQD 154

BLAST of Carg02145 vs. ExPASy TrEMBL
Match: A0A6J1K1N3 (uncharacterized protein LOC111489817 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489817 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.4e-70
Identity = 149/154 (96.75%), Postives = 153/154 (99.35%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNA+LHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNAALHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPM-VTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAI 120
           SIHPM VTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAI
Sbjct: 61  SIHPMQVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAI 120

Query: 121 AEIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           AEIEE+GEEAIDAQAVKEAVV+AIKIWSQSGEQD
Sbjct: 121 AEIEEEGEEAIDAQAVKEAVVQAIKIWSQSGEQD 154

BLAST of Carg02145 vs. ExPASy TrEMBL
Match: A0A6J1FFD0 (uncharacterized protein LOC111445214 isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111445214 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.2e-70
Identity = 149/153 (97.39%), Postives = 152/153 (99.35%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSREHNNASLHHNLQLLRSITNSHA LNKASIIVDASKYIEELKQKVERLNQDISTVQT
Sbjct: 1   MVSREHNNASLHHNLQLLRSITNSHA-LNKASIIVDASKYIEELKQKVERLNQDISTVQT 60

Query: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIA 120
           SIHPMVTVESLAKGFSINVFSEKSCQGLLVS+LEAFEELGLNVLEARVSCTDSFQLQAIA
Sbjct: 61  SIHPMVTVESLAKGFSINVFSEKSCQGLLVSILEAFEELGLNVLEARVSCTDSFQLQAIA 120

Query: 121 EIEEQGEEAIDAQAVKEAVVEAIKIWSQSGEQD 154
           EIEEQGEEAIDAQ+VKEAVV+AIKIWSQSGEQD
Sbjct: 121 EIEEQGEEAIDAQSVKEAVVQAIKIWSQSGEQD 152

BLAST of Carg02145 vs. TAIR 10
Match: AT2G40435.1 (BEST Arabidopsis thaliana protein match is: transcription regulators (TAIR:AT3G56220.1); Has 289 Blast hits to 289 proteins in 30 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 289; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 159.5 bits (402), Expect = 2.2e-39
Identity = 95/154 (61.69%), Postives = 113/154 (73.38%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           MVSRE    SL    QLLRSITNSHA+ N  SII+DASKYI++LKQKVER NQD +  Q+
Sbjct: 1   MVSREQKRGSLQEKFQLLRSITNSHAE-NDTSIIMDASKYIQKLKQKVERFNQDPTAEQS 60

Query: 61  SIH------PMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSF 120
           S        PMVTVE+L KGF INVFS K+  G+LVSVLEAFE++GLNVLEAR SCTDSF
Sbjct: 61  SSEPTDPKTPMVTVETLDKGFMINVFSGKNQPGMLVSVLEAFEDIGLNVLEARASCTDSF 120

Query: 121 QLQAIAEIEEQGEEAIDAQAVKEAVVEAIKIWSQ 149
            L A+    E GE  +DA+AVK+AV +AI+ W +
Sbjct: 121 SLHAMGLENEDGEN-MDAEAVKQAVTDAIRSWGE 152

BLAST of Carg02145 vs. TAIR 10
Match: AT3G56220.1 (transcription regulators )

HSP 1 Score: 145.2 bits (365), Expect = 4.3e-35
Identity = 87/155 (56.13%), Postives = 111/155 (71.61%), Query Frame = 0

Query: 1   MVSREH-NNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQ 60
           MVSREH   +SL     LLRSIT+SHA+ ++ SIIVDASKYI++LKQKVE++N   ++ Q
Sbjct: 1   MVSREHKRGSSLREKFHLLRSITDSHAE-SETSIIVDASKYIKKLKQKVEKINNATTSEQ 60

Query: 61  -----TSIHPMVTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDSF 120
                +  +PMVTVE+L KGF I V S K+  G+LV VLE FE+LGL+V+EARVSCTD+F
Sbjct: 61  SFRESSDPNPMVTVETLEKGFMIKVMSRKNEAGMLVCVLETFEDLGLDVVEARVSCTDTF 120

Query: 121 QLQAIAEIEEQGEEAIDAQAVKEAVVEAIKIWSQS 150
            L AI        + IDA+AVK+AV EAI+ WS S
Sbjct: 121 SLHAIGSSNNDDGDCIDAEAVKQAVAEAIRTWSDS 154

BLAST of Carg02145 vs. TAIR 10
Match: AT1G29270.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G40435.1); Has 98 Blast hits to 98 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 98; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 61.6 bits (148), Expect = 6.3e-10
Identity = 42/126 (33.33%), Postives = 73/126 (57.94%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIV-DASKYIEELKQKVERLNQDIS--- 60
           MV+ E    +       L+++T+    +++ S+++ +A  YI  LK ++E L ++     
Sbjct: 1   MVASEQKKRASQGKPHFLKNLTHFKFSIHEQSMVIREALLYIAMLKLEIEALQREYEDLK 60

Query: 61  -TVQTSIHPM--VTVESLAKGFSINVFSEKSCQGLLVSVLEAFEELGLNVLEARVSCTDS 120
            T + S+H    V VE + + F + + S +  +  LV++LEAFEE+GLNV +AR SC DS
Sbjct: 61  ITKKESLHQFQEVKVEKIGEMFQVKIKSPRG-ENNLVNILEAFEEMGLNVAQARASCLDS 120

BLAST of Carg02145 vs. TAIR 10
Match: AT5G10570.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.3e-07
Identity = 43/154 (27.92%), Postives = 78/154 (50.65%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTVQT 60
           +++       L+  L LLRSI     ++++ SI+ DA  Y++EL  K+ +L +D   + +
Sbjct: 153 LMAERRRRKRLNDRLSLLRSIVPKITKMDRTSILGDAIDYMKELLDKINKLQEDEQELGS 212

Query: 61  SIH--PMVTVESLAKGF--------SINVFSEKSC---QGLLVSVLEAFEELGLNVLEAR 120
           + H   ++T ES+ +           +N   +  C    GL+VS +   E LGL + +  
Sbjct: 213 NSHLSTLITNESMVRNSLKFEVDQREVNTHIDICCPTKPGLVVSTVSTLETLGLEIEQCV 272

Query: 121 VSCTDSFQLQAIA-EIEEQGEEAIDAQAVKEAVV 141
           +SC   F LQA   E+ EQ    + ++A K+A++
Sbjct: 273 ISCFSDFSLQASCFEVGEQ-RYMVTSEATKQALI 305

BLAST of Carg02145 vs. TAIR 10
Match: AT1G12860.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 6.1e-05
Identity = 39/175 (22.29%), Postives = 84/175 (48.00%), Query Frame = 0

Query: 1   MVSREHNNASLHHNLQLLRSITNSHAQLNKASIIVDASKYIEELKQKVERLNQDISTV-- 60
           +++       L+  L +LRS+    +++++ASI+ DA  Y++EL Q++  L+ ++ +   
Sbjct: 269 LMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHTELESTPP 328

Query: 61  -QTSIHPMV-TVESLA------------------------------KGFSINVFSEKSCQ 120
             +S+HP+  T ++L+                              K  +I++F  +   
Sbjct: 329 SSSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLREGKAVNIHMFCGRR-P 388

Query: 121 GLLVSVLEAFEELGLNVLEARVSCTDSFQLQAIAEIEEQGEEAIDAQAVKEAVVE 142
           GLL+S + A + LGL+V +A +SC + F L      + Q +  +  + +K  +++
Sbjct: 389 GLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQCQEDHDVLPEQIKAVLLD 442

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016033.11.4e-72100.00Transcription factor bHLH61 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023551078.14.1e-7298.69uncharacterized protein LOC111809011 isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022939239.19.2e-7298.04uncharacterized protein LOC111445214 isoform X3 [Cucurbita moschata][more]
XP_022993986.12.1e-7197.39uncharacterized protein LOC111489817 isoform X3 [Cucurbita maxima][more]
XP_023551076.11.0e-7098.05uncharacterized protein LOC111809011 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LXA91.8e-0627.92Transcription factor bHLH61 OS=Arabidopsis thaliana OX=3702 GN=BHLH61 PE=2 SV=1[more]
Q10S446.5e-0420.63Transcription factor BHLH3 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH3 PE=... [more]
Q9LPW38.5e-0422.29Transcription factor SCREAM2 OS=Arabidopsis thaliana OX=3702 GN=SCRM2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FGK64.5e-7298.04uncharacterized protein LOC111445214 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JXW19.9e-7297.39uncharacterized protein LOC111489817 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FG891.1e-7097.40uncharacterized protein LOC111445214 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K1N32.4e-7096.75uncharacterized protein LOC111489817 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1FFD03.2e-7097.39uncharacterized protein LOC111445214 isoform X4 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT2G40435.12.2e-3961.69BEST Arabidopsis thaliana protein match is: transcription regulators (TAIR:AT3G5... [more]
AT3G56220.14.3e-3556.13transcription regulators [more]
AT1G29270.16.3e-1033.33unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G10570.11.3e-0727.92basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G12860.16.1e-0522.29basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 34..61
NoneNo IPR availablePANTHERPTHR31945:SF5TRANSCRIPTION FACTOR SCREAM-LIKE PROTEINcoord: 1..151
NoneNo IPR availablePANTHERPTHR31945TRANSCRIPTION FACTOR SCREAM2-RELATEDcoord: 1..151
NoneNo IPR availableCDDcd04873ACT_UUR-ACR-likecoord: 87..143
e-value: 0.00957476
score: 31.3645
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 4..69
e-value: 7.6E-6
score: 27.9
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 11..70

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02145-RACarg02145-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity