Carg01997 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01997
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	aspartyl protease AED3
Location	Carg_Chr04: 8139582 .. 8141934 (-)
RNA-Seq Expression	Carg01997
Synteny	Carg01997

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGACAAAATCCCCCTTCTTCCTCGCTCTCCTCCTCCTCCTCTCCATATCGGCCGAATCCCACCACCACCACCACCACCACCAACACCCCAACTGCAACGCCGCCGCCGACCGCAGCTCTACCCTCCAAGTGTTCCACATTTTCAGCCCCTGTTCCCCCTTCCGCCCCTCCAAGCCGCTGTCATGGGCCGACAATGTGCTCCAAATGCAGGCCAAGGACCAAGCCCGCCTCCAGTTCCTGTCCTCCCTTGTCGCCCGCAAGTCCGTCGCCCCCATTGCCTCCGCCCGCCAGCTCATCCAGAGCCCCACCTTCGTAGTCCGAGCCAAGATCGGCACCCCTGCTCAGACCCTCCTCATGGCCCTCGATACCAGCAACGACGCCGCTTGGATTCCCTGTTCCGGCTGTGTCGGCTGCCCATCTACCACCGTCTTCTCCTCCGATAAGTCCTCCTCCTTCCGCCCCCTCCCCTGCCGATCCCCTCAATGCAACCAGGTACCTACCTCTGTTCTATTTCAAATCAATTTTGAATTTTTGCTGCTTTTATTTTTCCAACTCACCAATGTGGGGGCATTTCAATTCCCATATTCTACATCAAACCTTAATTATCGAAGTGCACGCGCTCACGCGCCGCCTGTATCGGTGGAGAGGCCAGATATCCGTTGGAATATTCAATGATTATAAACCTACCTTTATTCACCCACATAGCATTCCCCTTGTATTTGGTAATATGTGGGGTTCCCCTCTTTATTATGCTTTTTTTTTAAAAAAAAAATAATAATAATAATACTTTTATTTTATTTCTAAATCCAATAAAATAAATTAATTGAATATATTTTTTTTTGAAAATTAATAAGAACTTCGGATGCTTTTGTAGAATCAAATCCATGTTCCCACGTGCCCAGTGTCCTTTCCTTGAAGGTGACACGTGTGTCCAAAAACTCCATTCCTCGTGATACGAGCCCCTCTTTATCACGTCAATCATGTAACAAATCAACTATTTCTTAACATTATTTTAAAAGAAAGGAATTAATTTCGATAAAAGTTAAATAATAAATTATTTGTTTATTTTTTAATTTTTGGATTTCTCTAGAATCTATGACTGTCAGTGAGTCACCGACGGGAACGTTCAGTCACGGGCCAAACAACTTCTTCCCAACAGTACTTATCGTATTATTAAACATCGCACGTTGACGGCACGTGACCCTTTTGTTTATCAGGTACCGAACCCCAGTTGCAGCAGTAGCGCGTGCGGCTTCAACTTGACGTACGGCAGCTCGACTGTAGCGGCGGATCTGGTTCAAGACAACATAACTCTAGCCAACGACTCGGTTCCGGCGTACACATTTGGGTGTATCACGAAGGCGACGGGTAGTTCAGTGCCGCCGCAGGGACTATTGGGCCTGGGTCGAGGCCCATTATCGCTTTTGGGCCAGAGCCAGAGTCTGTACCGGTCCACATTCTCGTACTGCCTTCCGAGCTTCAAATCGACCGGCTTTTCTGGGTCGTTGCGTCTTGGGCCTGTGGCCCAGCCCAAGAGGATTAAGTACACGCCGCTGCTCCGAAACCCGAGGCGGTCGTCTTTGTATTATGTGAATCTAATCGCGATCAGAGTCGGCCGGCGAATCGTCGATATTCCTCCCTCTGCTCTTGCTTTTAACTCCGCCACCGGCGCCGGCACCATCATCGATTCCGGTATCGATTTAAATAGGTTTTAAAAATATTTTAGACTAAATTAATAAAAATACACTTTTAATTTTGAAAATATCTTTAATTCAGGGACGACATTCACAAGGCTGGTGGCACCGGCGTACACGGCGGTGAGGAACGAATTCCGGCGAAGAATAGGCAACGCAACCATCTCATCCCTCGGCGGCTTCGACACGTGCTACACAGTCCCCATCATCTCTCCCACCATAACCTTCATGTTCGCCGGAATGAACGTCACTCTTCCGCCGGACAACTTCCTCCTCCACAGCTCCGCCGGAACCACCACCTGCCTCGCCATGGCCGCCGCCCCGGATAACGTCAACTCCGTACTCAACGTCATTGCCAGCATGCAGCAGCAGAACCACCGCATTCTCTTCGACATTCCAAATTCCCGAATCGGCGTCGCTCGCGAACCCTGCTCTTAAACCCCCGTCGTCCGCCGTATCATCTCCAGCTCCGGTGATATTTATTTTTGTTGGTTTTAGAATAATAAGAATGTGTGGTCATCATGGTTTGGTGGGGGGATGGGGAGAAGGGTAAAGTAGTAATTTTGTATGGCTGTTTTTATAGTTACGTTGTTTGGGATTCTTAAGGTTTTAAGCTTATTATATTATGGATGTCTGAAAAAGCAAACCTGTTTTTT

mRNA sequence

ATGAAGACAAAATCCCCCTTCTTCCTCGCTCTCCTCCTCCTCCTCTCCATATCGGCCGAATCCCACCACCACCACCACCACCACCAACACCCCAACTGCAACGCCGCCGCCGACCGCAGCTCTACCCTCCAAGTGTTCCACATTTTCAGCCCCTGTTCCCCCTTCCGCCCCTCCAAGCCGCTGTCATGGGCCGACAATGTGCTCCAAATGCAGGCCAAGGACCAAGCCCGCCTCCAGTTCCTGTCCTCCCTTGTCGCCCGCAAGTCCGTCGCCCCCATTGCCTCCGCCCGCCAGCTCATCCAGAGCCCCACCTTCGTAGTCCGAGCCAAGATCGGCACCCCTGCTCAGACCCTCCTCATGGCCCTCGATACCAGCAACGACGCCGCTTGGATTCCCTGTTCCGGCTGTGTCGGCTGCCCATCTACCACCGTCTTCTCCTCCGATAAGTCCTCCTCCTTCCGCCCCCTCCCCTGCCGATCCCCTCAATGCAACCAGGTACCTACCTCTGTTCTATTTCAAATCAATTTTGAATTTTTGCTGCTTTTATTTTTCCAACTCACCAATTCACGGGCCAAACAACTTCTTCCCAACAGTACTTATCGTATTATTAAACATCGCACGTTGACGGCACGTGACCCTTTTGTTTATCAGGTACCGAACCCCAGTTGCAGCAGTAGCGCGTGCGGCTTCAACTTGACGTACGGCAGCTCGACTGTAGCGGCGGATCTGGTTCAAGACAACATAACTCTAGCCAACGACTCGGTTCCGGCGTACACATTTGGGTGTATCACGAAGGCGACGGGTAGTTCAGTGCCGCCGCAGGGACTATTGGGCCTGGGTCGAGGCCCATTATCGCTTTTGGGCCAGAGCCAGAGTCTGTACCGGTCCACATTCTCGTACTGCCTTCCGAGCTTCAAATCGACCGGCTTTTCTGGGTCGTTGCGTCTTGGGCCTGTGGCCCAGCCCAAGAGGATTAAGTACACGCCGCTGCTCCGAAACCCGAGGCGGTCGTCTTTGTATTATGTGAATCTAATCGCGATCAGAGTCGGCCGGCGAATCGTCGATATTCCTCCCTCTGCTCTTGCTTTTAACTCCGCCACCGGCGCCGGCACCATCATCGATTCCGGGACGACATTCACAAGGCTGGTGGCACCGGCGTACACGGCGGTGAGGAACGAATTCCGGCGAAGAATAGGCAACGCAACCATCTCATCCCTCGGCGGCTTCGACACGTGCTACACAGTCCCCATCATCTCTCCCACCATAACCTTCATGTTCGCCGGAATGAACGTCACTCTTCCGCCGGACAACTTCCTCCTCCACAGCTCCGCCGGAACCACCACCTGCCTCGCCATGGCCGCCGCCCCGGATAACGTCAACTCCGTACTCAACGTCATTGCCAGCATGCAGCAGCAGAACCACCGCATTCTCTTCGACATTCCAAATTCCCGAATCGGCGTCGCTCGCGAACCCTGCTCTTAAACCCCCGTCGTCCGCCGTATCATCTCCAGCTCCGGTGATATTTATTTTTGTTGGTTTTAGAATAATAAGAATGTGTGGTCATCATGGTTTGGTGGGGGGATGGGGAGAAGGGTAAAGTAGTAATTTTGTATGGCTGTTTTTATAGTTACGTTGTTTGGGATTCTTAAGGTTTTAAGCTTATTATATTATGGATGTCTGAAAAAGCAAACCTGTTTTTT

Coding sequence (CDS)

ATGAAGACAAAATCCCCCTTCTTCCTCGCTCTCCTCCTCCTCCTCTCCATATCGGCCGAATCCCACCACCACCACCACCACCACCAACACCCCAACTGCAACGCCGCCGCCGACCGCAGCTCTACCCTCCAAGTGTTCCACATTTTCAGCCCCTGTTCCCCCTTCCGCCCCTCCAAGCCGCTGTCATGGGCCGACAATGTGCTCCAAATGCAGGCCAAGGACCAAGCCCGCCTCCAGTTCCTGTCCTCCCTTGTCGCCCGCAAGTCCGTCGCCCCCATTGCCTCCGCCCGCCAGCTCATCCAGAGCCCCACCTTCGTAGTCCGAGCCAAGATCGGCACCCCTGCTCAGACCCTCCTCATGGCCCTCGATACCAGCAACGACGCCGCTTGGATTCCCTGTTCCGGCTGTGTCGGCTGCCCATCTACCACCGTCTTCTCCTCCGATAAGTCCTCCTCCTTCCGCCCCCTCCCCTGCCGATCCCCTCAATGCAACCAGGTACCTACCTCTGTTCTATTTCAAATCAATTTTGAATTTTTGCTGCTTTTATTTTTCCAACTCACCAATTCACGGGCCAAACAACTTCTTCCCAACAGTACTTATCGTATTATTAAACATCGCACGTTGACGGCACGTGACCCTTTTGTTTATCAGGTACCGAACCCCAGTTGCAGCAGTAGCGCGTGCGGCTTCAACTTGACGTACGGCAGCTCGACTGTAGCGGCGGATCTGGTTCAAGACAACATAACTCTAGCCAACGACTCGGTTCCGGCGTACACATTTGGGTGTATCACGAAGGCGACGGGTAGTTCAGTGCCGCCGCAGGGACTATTGGGCCTGGGTCGAGGCCCATTATCGCTTTTGGGCCAGAGCCAGAGTCTGTACCGGTCCACATTCTCGTACTGCCTTCCGAGCTTCAAATCGACCGGCTTTTCTGGGTCGTTGCGTCTTGGGCCTGTGGCCCAGCCCAAGAGGATTAAGTACACGCCGCTGCTCCGAAACCCGAGGCGGTCGTCTTTGTATTATGTGAATCTAATCGCGATCAGAGTCGGCCGGCGAATCGTCGATATTCCTCCCTCTGCTCTTGCTTTTAACTCCGCCACCGGCGCCGGCACCATCATCGATTCCGGGACGACATTCACAAGGCTGGTGGCACCGGCGTACACGGCGGTGAGGAACGAATTCCGGCGAAGAATAGGCAACGCAACCATCTCATCCCTCGGCGGCTTCGACACGTGCTACACAGTCCCCATCATCTCTCCCACCATAACCTTCATGTTCGCCGGAATGAACGTCACTCTTCCGCCGGACAACTTCCTCCTCCACAGCTCCGCCGGAACCACCACCTGCCTCGCCATGGCCGCCGCCCCGGATAACGTCAACTCCGTACTCAACGTCATTGCCAGCATGCAGCAGCAGAACCACCGCATTCTCTTCGACATTCCAAATTCCCGAATCGGCGTCGCTCGCGAACCCTGCTCTTAA

Protein sequence

MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPCS

Homology

[Close]

BLAST of Carg01997 vs. NCBI nr
Match: KAG7031923.1 (Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 973.8 bits (2516), Expect = 5.6e-280
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 0


Query: 1   MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60
           MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP
Sbjct: 1   MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60

Query: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120
           LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM
Sbjct: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120

Query: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLL 180
           ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLL
Sbjct: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLL 180

Query: 181 LLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVA 240
           LLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVA
Sbjct: 181 LLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVA 240

Query: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300
           ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY
Sbjct: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300

Query: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360
           CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA
Sbjct: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360

Query: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420
           LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP
Sbjct: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420

Query: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI 480
           TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI
Sbjct: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI 480

Query: 481 PNSRIGVAREPCS 494
           PNSRIGVAREPCS
Sbjct: 481 PNSRIGVAREPCS 493

[Close]

BLAST of Carg01997 vs. NCBI nr
Match: KAG6601125.1 (Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 849.4 bits (2193), Expect = 1.6e-242
Identity = 441/493 (89.45%), Postives = 441/493 (89.45%), Query Frame = 0


Query: 1   MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60
           MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP
Sbjct: 1   MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60

Query: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120
           LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM
Sbjct: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120

Query: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLL 180
           ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN                
Sbjct: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN---------------- 180

Query: 181 LLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVA 240
                                               QVPNPSCSSSACGFNLTYGSSTVA
Sbjct: 181 ------------------------------------QVPNPSCSSSACGFNLTYGSSTVA 240

Query: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300
           ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY
Sbjct: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300

Query: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360
           CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA
Sbjct: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360

Query: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420
           LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP
Sbjct: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420

Query: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI 480
           TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI
Sbjct: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI 441

Query: 481 PNSRIGVAREPCS 494
           PNSRIGVAREPCS
Sbjct: 481 PNSRIGVAREPCS 441

[Close]

BLAST of Carg01997 vs. NCBI nr
Match: XP_023526864.1 (aspartyl protease AED3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 837.8 bits (2163), Expect = 4.8e-239
Identity = 437/494 (88.46%), Postives = 440/494 (89.07%), Query Frame = 0


Query: 1   MKTKSPFFLA-LLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK 60
           MKTKSPFFLA LLLLLSISAES HHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK
Sbjct: 1   MKTKSPFFLALLLLLLSISAESLHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK 60

Query: 61  PLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLL 120
           PLSWADNVLQMQAKDQARLQFLSSLVARKSVAPI+SARQLIQSPTFVVRAKIGTPAQTLL
Sbjct: 61  PLSWADNVLQMQAKDQARLQFLSSLVARKSVAPISSARQLIQSPTFVVRAKIGTPAQTLL 120

Query: 121 MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFL 180
           MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN               
Sbjct: 121 MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN--------------- 180

Query: 181 LLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTV 240
                                                QVPNPSCSSS+CGFNLTYGSSTV
Sbjct: 181 -------------------------------------QVPNPSCSSSSCGFNLTYGSSTV 240

Query: 241 AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS 300
           AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS
Sbjct: 241 AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS 300

Query: 301 YCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS 360
           YCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS
Sbjct: 301 YCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS 360

Query: 361 ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS 420
           ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS
Sbjct: 361 ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS 420

Query: 421 PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 480
           PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD
Sbjct: 421 PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 442

Query: 481 IPNSRIGVAREPCS 494
           +PNSRIGVAREPCS
Sbjct: 481 VPNSRIGVAREPCS 442

[Close]

BLAST of Carg01997 vs. NCBI nr
Match: XP_022957241.1 (aspartyl protease AED3 [Cucurbita moschata])

HSP 1 Score: 830.1 bits (2143), Expect = 1.0e-236
Identity = 434/493 (88.03%), Postives = 436/493 (88.44%), Query Frame = 0


Query: 1   MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60
           MKTKSPF L+LLLLLSISAESHHHH    HPNCNAAADRSSTLQVFHIFSPCSPFRPSKP
Sbjct: 1   MKTKSPFLLSLLLLLSISAESHHHH----HPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60

Query: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120
           LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM
Sbjct: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120

Query: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLL 180
           ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN                
Sbjct: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN---------------- 180

Query: 181 LLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVA 240
                                               QVPNPSCSSSACGFNLTYGSSTVA
Sbjct: 181 ------------------------------------QVPNPSCSSSACGFNLTYGSSTVA 240

Query: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300
           ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY
Sbjct: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300

Query: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360
           CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA
Sbjct: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360

Query: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420
           LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP
Sbjct: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420

Query: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI 480
           TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD+
Sbjct: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDV 437

Query: 481 PNSRIGVAREPCS 494
           PNSRIGVAREPCS
Sbjct: 481 PNSRIGVAREPCS 437

[Close]

BLAST of Carg01997 vs. NCBI nr
Match: XP_022988941.1 (aspartyl protease AED3-like [Cucurbita maxima])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 430/494 (87.04%), Postives = 434/494 (87.85%), Query Frame = 0


Query: 1   MKTKSPFFLA-LLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK 60
           MKTKSPF L+ LLLLLSISAES HHHH   HPNCNAAADRSSTLQVFHIFSPCSPFRPSK
Sbjct: 1   MKTKSPFLLSLLLLLLSISAESLHHHH---HPNCNAAADRSSTLQVFHIFSPCSPFRPSK 60

Query: 61  PLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLL 120
           PLSWADNVLQMQAKDQARLQFLSSLVA KSVAPIASARQLIQSPTFVVRAKIGTPAQTLL
Sbjct: 61  PLSWADNVLQMQAKDQARLQFLSSLVACKSVAPIASARQLIQSPTFVVRAKIGTPAQTLL 120

Query: 121 MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFL 180
           MALDTSNDAAWIPCSGCVGCPSTTVFSSDKS+SFRPLPCRSPQCN               
Sbjct: 121 MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFRPLPCRSPQCN--------------- 180

Query: 181 LLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTV 240
                                                QVPNPSCSSSACGFNLTYGSSTV
Sbjct: 181 -------------------------------------QVPNPSCSSSACGFNLTYGSSTV 240

Query: 241 AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS 300
           AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS
Sbjct: 241 AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS 300

Query: 301 YCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS 360
           YCLPSFKST FSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS
Sbjct: 301 YCLPSFKSTSFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS 360

Query: 361 ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS 420
           ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS
Sbjct: 361 ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS 420

Query: 421 PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 480
           PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD
Sbjct: 421 PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 439

Query: 481 IPNSRIGVAREPCS 494
           +PNSR+GVAREPCS
Sbjct: 481 VPNSRMGVAREPCS 439

[Close]

BLAST of Carg01997 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.0e-110
Identity = 230/495 (46.46%), Postives = 302/495 (61.01%), Query Frame = 0


Query: 7   FFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK-PLSWAD 66
           FFL LLL  + +  +       +     AA D S  L +  I + CSPF P+    S  D
Sbjct: 10  FFLTLLLPFTFTTAT-------RDTCATAAPDGSDDLSIIPINAKCSPFAPTHVSASVID 69

Query: 67  NVLQMQAKDQARLQFLSSLVARK---SVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 126
            VL M + D  RL +LSSLVA K   +  P+AS  QL     +VVRAK+GTP Q + M L
Sbjct: 70  TVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPPQLMFMVL 129

Query: 127 DTSNDAAWIPCSGCVGCP-STTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLL 186
           DTSNDA W+PCSGC GC  ++T F+++ SS++  + C + QC Q                
Sbjct: 130 DTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQA--------------- 189

Query: 187 LFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTY-GSSTVA 246
                                   R LT         P+ S   S C FN +Y G S+ +
Sbjct: 190 ------------------------RGLTC--------PSSSPQPSVCSFNQSYGGDSSFS 249

Query: 247 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 306
           A LVQD +TLA D +P ++FGCI  A+G+S+PPQGL+GLGRGP+SL+ Q+ SLY   FSY
Sbjct: 250 ASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTSLYSGVFSY 309

Query: 307 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 366
           CLPSF+S  FSGSL+LG + QPK I+YTPLLRNPRR SLYYVNL  + VG   V + P  
Sbjct: 310 CLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGVSVGSVQVPVDPVY 369

Query: 367 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTV--PII 426
           L F++ +GAGTIIDSGT  TR   P Y A+R+EFR+++  ++ S+LG FDTC++     +
Sbjct: 370 LTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTLGAFDTCFSADNENV 429

Query: 427 SPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILF 486
           +P IT     +++ LP +N L+HSSAGT TCL+MA    N N+VLNVIA++QQQN RILF
Sbjct: 430 APKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRILF 449

Query: 487 DIPNSRIGVAREPCS 494
           D+PNSRIG+A EPC+
Sbjct: 490 DVPNSRIGIAPEPCN 449

[Close]

BLAST of Carg01997 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 6.4e-93
Identity = 202/468 (43.16%), Postives = 278/468 (59.40%), Query Frame = 0


Query: 37  ADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSV--APIA 96
           A  ++ L V+H   P SP     PL   ++++ +   D ARL FLSS  A   V  AP+A
Sbjct: 19  AAAAAELSVYHNVHPSSP----SPL---ESIIALARDDDARLLFLSSKAATAGVSSAPVA 78

Query: 97  SARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFR 156
           S +     P++VVRA +G+P+Q LL+ALDTS DA W  CS C  CPS+++F+   SSS+ 
Sbjct: 79  SGQ---APPSYVVRAGLGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANSSSYA 138

Query: 157 PLPCRSPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPF 216
            LPC S  C                    FQ     A Q   ++                
Sbjct: 139 SLPCSSSWCP------------------LFQGQACPAPQGGGDAA--------------- 198

Query: 217 VYQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKATG--SSVP 276
                 P  +   C F+  +  ++  A L  D + L  D++P YTFGC++  TG  +++P
Sbjct: 199 -----PPPATLPTCAFSKPFADASFQAALASDTLRLGKDAIPNYTFGCVSSVTGPTTNMP 258

Query: 277 PQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGP-VAQPKRIKYTPLL 336
            QGLLGLGRGP++LL Q+ SLY   FSYCLPS++S  FSGSLRLG    QP+ ++YTP+L
Sbjct: 259 RQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYRSYYFSGSLRLGAGGGQPRSVRYTPML 318

Query: 337 RNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVR 396
           RNP RSSLYYVN+  + VG   V +P  + AF++ATGAGT++DSGT  TR  AP Y A+R
Sbjct: 319 RNPHRSSLYYVNVTGLSVGHAWVKVPAGSFAFDAATGAGTVVDSGTVITRWTAPVYAALR 378

Query: 397 NEFRRRIGNAT-ISSLGGFDTCYTVPII----SPTIT-FMFAGMNVTLPPDNFLLHSSAG 456
            EFRR++   +  +SLG FDTC+    +    +P +T  M  G+++ LP +N L+HSSA 
Sbjct: 379 EEFRRQVAAPSGYTSLGAFDTCFNTDEVAAGGAPAVTVHMDGGVDLALPMENTLIHSSAT 438

Query: 457 TTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPCS 494
              CLAMA AP NVNSV+NVIA++QQQN R++FD+ NSR+G A+E C+
Sbjct: 439 PLACLAMAEAPQNVNSVVNVIANLQQQNIRVVFDVANSRVGFAKESCN 438

[Close]

BLAST of Carg01997 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 5.1e-42
Identity = 119/403 (29.53%), Postives = 182/403 (45.16%), Query Frame = 0


Query: 102 SPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGC--PSTTVFSSDKSSSFRPLPCR 161
           S  +  R  +GTPA+ + M LDT +D  W+ C+ C  C   S  +F   KS ++  +PC 
Sbjct: 139 SGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCS 198

Query: 162 SPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVP 221
           SP C ++ ++                  N+R K                           
Sbjct: 199 SPHCRRLDSAG----------------CNTRRK--------------------------- 258

Query: 222 NPSCSSSACGFNLTYG-SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLG 281
                   C + ++YG  S    D   + +T   + V     GC     G  V   GLLG
Sbjct: 259 -------TCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLG 318

Query: 282 LGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSS 341
           LG+G LS  GQ+   +   FSYCL    ++    S+  G  A  +  ++TPLL NP+  +
Sbjct: 319 LGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDT 378

Query: 342 LYYVNLIAIRV-GRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRR 401
            YYV L+ I V G R+  +  S    +     G IIDSGT+ TRL+ PAY A+R+ F  R
Sbjct: 379 FYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--R 438

Query: 402 IGNATIS---SLGGFDTCYTVPIIS----PTITFMFAGMNVTLPPDNFLLHSSAGTTTCL 461
           +G  T+        FDTC+ +  ++    PT+   F G +V+LP  N+L+        C 
Sbjct: 439 VGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCF 485

Query: 462 AMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPCS 494
           A A         L++I ++QQQ  R+++D+ +SR+G A   C+
Sbjct: 499 AFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Carg01997 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 8.5e-37
Identity = 116/400 (29.00%), Postives = 166/400 (41.50%), Query Frame = 0


Query: 102 SPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGC--PSTTVFSSDKSSSFRPLPCR 161
           S  + VR  +G+P +   M +D+ +D  W+ C  C  C   S  VF   KS S+  + C 
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187

Query: 162 SPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVP 221
           S  C+                                                    ++ 
Sbjct: 188 SSVCD----------------------------------------------------RIE 247

Query: 222 NPSCSSSACGFNLTYG-SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLG 281
           N  C S  C + + YG  S     L  + +T A   V     GC  +  G  +   GLLG
Sbjct: 248 NSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMFIGAAGLLG 307

Query: 282 LGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSS 341
           +G G +S +GQ        F YCL S + T  +GSL  G  A P    + PL+RNPR  S
Sbjct: 308 IGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWVPLVRNPRAPS 367

Query: 342 LYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRI 401
            YYV L  + VG   + +P            G ++D+GT  TRL   AY A R+ F+ + 
Sbjct: 368 FYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQT 427

Query: 402 GN-ATISSLGGFDTCYT----VPIISPTITFMFA-GMNVTLPPDNFLLHSSAGTTTCLAM 461
            N    S +  FDTCY     V +  PT++F F  G  +TLP  NFL+      T C A 
Sbjct: 428 ANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAF 470

Query: 462 AAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPC 493
           AA+P      L++I ++QQ+  ++ FD  N  +G     C
Sbjct: 488 AASPTG----LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Carg01997 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.9e-36
Identity = 133/476 (27.94%), Postives = 205/476 (43.07%), Query Frame = 0


Query: 36  AADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIAS 95
           A++  S+L+V H+   CS       +   D +++   +DQAR++ + S +++ S   ++ 
Sbjct: 58  ASNTKSSLRVVHMHGACSHLSSDARVD-HDEIIR---RDQARVESIYSKLSKNSANEVSE 117

Query: 96  ARQ---------LIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVG-CPS--TT 155
           A+           + S  ++V   IGTP   L +  DT +D  W  C  C+G C S    
Sbjct: 118 AKSTELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEP 177

Query: 156 VFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRII 215
            F+   SS+++ + C SP C                                        
Sbjct: 178 KFNPSSSSTYQNVSCSSPMCEDA------------------------------------- 237

Query: 216 KHRTLTARDPFVYQVPNPSCSSSACGFNLTYG-SSTVAADLVQDNITLAN-DSVPAYTFG 275
                             SCS+S C +++ YG  S     L ++  TL N D +    FG
Sbjct: 238 -----------------ESCSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFG 297

Query: 276 CITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQ 335
           C     G      GLLGLG G LSL  Q+ + Y + FSYCLPSF S   +G L  G    
Sbjct: 298 CGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNS-TGHLTFGSAGI 357

Query: 336 PKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTR 395
            + +K+TP+   P   + Y +++I I VG + + I P     NS +  G IIDSGT FTR
Sbjct: 358 SESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITP-----NSFSTEGAIIDSGTVFTR 417

Query: 396 LVAPAYTAVRNEFRRRIGN-ATISSLGGFDTCYTV----PIISPTITFMFAGMNVTLPPD 455
           L    Y  +R+ F+ ++ +  + S  G FDTCY       +  PTI F FAG  V     
Sbjct: 418 LPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDG 464

Query: 456 NFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPC 493
           + +      +  CLA A   D    +  +  ++QQ    +++D+   R+G A   C
Sbjct: 478 SGISLPIKISQVCLAFAGNDD----LPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of Carg01997 vs. ExPASy TrEMBL
Match: A0A6J1GYP2 (aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 4.9e-237
Identity = 434/493 (88.03%), Postives = 436/493 (88.44%), Query Frame = 0


Query: 1   MKTKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60
           MKTKSPF L+LLLLLSISAESHHHH    HPNCNAAADRSSTLQVFHIFSPCSPFRPSKP
Sbjct: 1   MKTKSPFLLSLLLLLSISAESHHHH----HPNCNAAADRSSTLQVFHIFSPCSPFRPSKP 60

Query: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120
           LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM
Sbjct: 61  LSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLM 120

Query: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLL 180
           ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN                
Sbjct: 121 ALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCN---------------- 180

Query: 181 LLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVA 240
                                               QVPNPSCSSSACGFNLTYGSSTVA
Sbjct: 181 ------------------------------------QVPNPSCSSSACGFNLTYGSSTVA 240

Query: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300
           ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY
Sbjct: 241 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 300

Query: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360
           CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA
Sbjct: 301 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 360

Query: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420
           LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP
Sbjct: 361 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISP 420

Query: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDI 480
           TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD+
Sbjct: 421 TITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDV 437

Query: 481 PNSRIGVAREPCS 494
           PNSRIGVAREPCS
Sbjct: 481 PNSRIGVAREPCS 437

[Close]

BLAST of Carg01997 vs. ExPASy TrEMBL
Match: A0A6J1JIM3 (aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.6e-233
Identity = 430/494 (87.04%), Postives = 434/494 (87.85%), Query Frame = 0


Query: 1   MKTKSPFFLA-LLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK 60
           MKTKSPF L+ LLLLLSISAES HHHH   HPNCNAAADRSSTLQVFHIFSPCSPFRPSK
Sbjct: 1   MKTKSPFLLSLLLLLLSISAESLHHHH---HPNCNAAADRSSTLQVFHIFSPCSPFRPSK 60

Query: 61  PLSWADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLL 120
           PLSWADNVLQMQAKDQARLQFLSSLVA KSVAPIASARQLIQSPTFVVRAKIGTPAQTLL
Sbjct: 61  PLSWADNVLQMQAKDQARLQFLSSLVACKSVAPIASARQLIQSPTFVVRAKIGTPAQTLL 120

Query: 121 MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFL 180
           MALDTSNDAAWIPCSGCVGCPSTTVFSSDKS+SFRPLPCRSPQCN               
Sbjct: 121 MALDTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFRPLPCRSPQCN--------------- 180

Query: 181 LLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTV 240
                                                QVPNPSCSSSACGFNLTYGSSTV
Sbjct: 181 -------------------------------------QVPNPSCSSSACGFNLTYGSSTV 240

Query: 241 AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS 300
           AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS
Sbjct: 241 AADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFS 300

Query: 301 YCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS 360
           YCLPSFKST FSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS
Sbjct: 301 YCLPSFKSTSFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPS 360

Query: 361 ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS 420
           ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS
Sbjct: 361 ALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIIS 420

Query: 421 PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 480
           PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD
Sbjct: 421 PTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFD 439

Query: 481 IPNSRIGVAREPCS 494
           +PNSR+GVAREPCS
Sbjct: 481 VPNSRMGVAREPCS 439

[Close]

BLAST of Carg01997 vs. ExPASy TrEMBL
Match: A0A5A7SU62 (Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003150 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.8e-214
Identity = 398/492 (80.89%), Postives = 412/492 (83.74%), Query Frame = 0


Query: 3   TKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 62
           TKSPF    LLLL +   S      H   NCN A DRSSTL+VFHIFSPCSPFRPSKPLS
Sbjct: 4   TKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKPLS 63

Query: 63  WADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 122
           WADNVLQMQAKDQARLQFLSSLVAR+SV PIASARQLIQSPTFVVRAKIGTPAQTLL+AL
Sbjct: 64  WADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 123

Query: 123 DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLL 182
           DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPC+SPQCN                  
Sbjct: 124 DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCN------------------ 183

Query: 183 FFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVAAD 242
                                             QVPNPSCS +ACGFNLTYGSSTVAAD
Sbjct: 184 ----------------------------------QVPNPSCSGTACGFNLTYGSSTVAAD 243

Query: 243 LVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL 302
           LVQDN+TLA DSVPAYTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL
Sbjct: 244 LVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL 303

Query: 303 PSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA 362
           PSFKS  FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA
Sbjct: 304 PSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA 363

Query: 363 FNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCYTVPIISPT 422
           FNSATGAGTIIDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCYTVPIISPT
Sbjct: 364 FNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPT 423

Query: 423 ITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 482
           ITFMFAGMNVTLPPDNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP
Sbjct: 424 ITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 443

Query: 483 NSRIGVAREPCS 494
           NSR+GVAREPCS
Sbjct: 484 NSRVGVAREPCS 443

[Close]

BLAST of Carg01997 vs. ExPASy TrEMBL
Match: A0A1S3BGB2 (aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103489519 PE=4 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.8e-214
Identity = 398/492 (80.89%), Postives = 412/492 (83.74%), Query Frame = 0


Query: 3   TKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 62
           TKSPF    LLLL +   S      H   NCN A DRSSTL+VFHIFSPCSPFRPSKPLS
Sbjct: 4   TKSPFLPLPLLLLLLLLFSISTAKSHIPLNCNPADDRSSTLKVFHIFSPCSPFRPSKPLS 63

Query: 63  WADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 122
           WADNVLQMQAKDQARLQFLSSLVAR+SV PIASARQLIQSPTFVVRAKIGTPAQTLL+AL
Sbjct: 64  WADNVLQMQAKDQARLQFLSSLVARRSVVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 123

Query: 123 DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLL 182
           DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPC+SPQCN                  
Sbjct: 124 DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCQSPQCN------------------ 183

Query: 183 FFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVAAD 242
                                             QVPNPSCS +ACGFNLTYGSSTVAAD
Sbjct: 184 ----------------------------------QVPNPSCSGTACGFNLTYGSSTVAAD 243

Query: 243 LVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL 302
           LVQDN+TLA DSVPAYTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL
Sbjct: 244 LVQDNVTLATDSVPAYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL 303

Query: 303 PSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA 362
           PSFKS  FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA
Sbjct: 304 PSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA 363

Query: 363 FNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCYTVPIISPT 422
           FNSATGAGTIIDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCYTVPIISPT
Sbjct: 364 FNSATGAGTIIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPT 423

Query: 423 ITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 482
           ITFMFAGMNVTLPPDNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP
Sbjct: 424 ITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 443

Query: 483 NSRIGVAREPCS 494
           NSR+GVAREPCS
Sbjct: 484 NSRVGVAREPCS 443

[Close]

BLAST of Carg01997 vs. ExPASy TrEMBL
Match: A0A0A0KTR5 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=4 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 3.8e-213
Identity = 394/492 (80.08%), Postives = 412/492 (83.74%), Query Frame = 0


Query: 3   TKSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLS 62
           TKSPF L LLLL   S  +      H   NCN AADRSSTLQVFHIFSPCSPFRPSKPLS
Sbjct: 4   TKSPFLLLLLLLHLFSIST---AKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLS 63

Query: 63  WADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 122
           WADNVLQMQAKDQARLQFLSSLVAR+S  PIASARQLIQSPTFVVRAKIGTPAQTLL+AL
Sbjct: 64  WADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 123

Query: 123 DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLL 182
           DTSNDAAWIPCSGC+GCPSTTVFSSDKSSSFRPLPC+SPQCN                  
Sbjct: 124 DTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCN------------------ 183

Query: 183 FFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTYGSSTVAAD 242
                                             QVPNPSCS SACGFNLTYGSSTVAAD
Sbjct: 184 ----------------------------------QVPNPSCSGSACGFNLTYGSSTVAAD 243

Query: 243 LVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYCL 302
           LVQDN+TLA DSVP+YTFGCI KATGSSVPPQGLLGLGRGPLSLLGQSQSLY+STFSYCL
Sbjct: 244 LVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCL 303

Query: 303 PSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALA 362
           PSFKS  FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLI+IRVGR+IVDIPPSALA
Sbjct: 304 PSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALA 363

Query: 363 FNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIG-NATISSLGGFDTCYTVPIISPT 422
           FNSATGAGT+IDSGTTFTRLVAPAYTAVR+EFRRR+G N T+SSLGGFDTCYTVPIISPT
Sbjct: 364 FNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPT 423

Query: 423 ITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 482
           ITFMFAGMNVTLPPDNFL+HS+AG+TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP
Sbjct: 424 ITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 440

Query: 483 NSRIGVAREPCS 494
           NSR+GVARE CS
Sbjct: 484 NSRVGVARESCS 440

[Close]

BLAST of Carg01997 vs. TAIR 10
Match: AT5G07030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-159
Identity = 282/470 (60.00%), Postives = 345/470 (73.40%), Query Frame = 0


Query: 30  HPNCN--AAADRSSTLQVFHIFSPCSPFRPSKPLSWADNVLQMQAKDQARLQFLSSLVAR 89
           HPNC+     D+ STL++FHI SPCSPF+ S PLSW   VLQ  A+DQARLQ+LSSLVA 
Sbjct: 38  HPNCDLTKTQDQGSTLRIFHIDSPCSPFKSSSPLSWEARVLQTLAQDQARLQYLSSLVAG 97

Query: 90  KSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTTVFSS 149
           +SV PIAS RQ++QS T++V+A IGTPAQ LL+A+DTS+D AWIPCSGCVGCPS T FS 
Sbjct: 98  RSVVPIASGRQMLQSTTYIVKALIGTPAQPLLLAMDTSSDVAWIPCSGCVGCPSNTAFSP 157

Query: 150 DKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRT 209
            KS+SF+ + C +PQC                                            
Sbjct: 158 AKSTSFKNVSCSAPQCK------------------------------------------- 217

Query: 210 LTARDPFVYQVPNPSCSSSACGFNLTYGSSTVAADLVQDNITLANDSVPAYTFGCITKAT 269
                    QVPNP+C + AC FNLTYGSS++AA+L QD I LA D + A+TFGC+ K  
Sbjct: 218 ---------QVPNPTCGARACSFNLTYGSSSIAANLSQDTIRLAADPIKAFTFGCVNKVA 277

Query: 270 GSSV--PPQGLLGLGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRI 329
           G     PPQGLLGLGRGPLSL+ Q+QS+Y+STFSYCLPSF+S  FSGSLRLGP +QP+R+
Sbjct: 278 GGGTIPPPQGLLGLGRGPLSLMSQAQSIYKSTFSYCLPSFRSLTFSGSLRLGPTSQPQRV 337

Query: 330 KYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAP 389
           KYT LLRNPRRSSLYYVNL+AIRVGR++VD+PP+A+AFN +TGAGTI DSGT +TRL  P
Sbjct: 338 KYTQLLRNPRRSSLYYVNLVAIRVGRKVVDLPPAAIAFNPSTGAGTIFDSGTVYTRLAKP 397

Query: 390 AYTAVRNEFRRRI--GNATISSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLLHSS 449
            Y AVRNEFR+R+    A ++SLGGFDTCY+  +  PTITFMF G+N+T+P DN +LHS+
Sbjct: 398 VYEAVRNEFRKRVKPTTAVVTSLGGFDTCYSGQVKVPTITFMFKGVNMTMPADNLMLHST 455

Query: 450 AGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPCS 494
           AG+T+CLAMAAAP+NVNSV+NVIASMQQQNHR+L D+PN R+G+ARE CS
Sbjct: 458 AGSTSCLAMAAAPENVNSVVNVIASMQQQNHRVLIDVPNGRLGLARERCS 455

[Close]

BLAST of Carg01997 vs. TAIR 10
Match: AT3G54400.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 544.3 bits (1401), Expect = 1.0e-154
Identity = 291/492 (59.15%), Postives = 348/492 (70.73%), Query Frame = 0


Query: 4   KSPFFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 63
           +S   + L+ LL + +ES          NCN  +  SS L+VFHI S CSPF+ S  +SW
Sbjct: 2   RSHLLILLISLLILKSES---------INCNEKS-HSSDLRVFHINSLCSPFKTS--VSW 61

Query: 64  ADNVLQMQAKDQARLQFLSSLV-ARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 123
           AD +LQ    D+AR  +LSSL   RKS  PIAS R ++QSPT++VRA IGTPAQ +L+AL
Sbjct: 62  ADTLLQ----DKARFLYLSSLAGVRKSSVPIASGRAIVQSPTYIVRANIGTPAQPMLVAL 121

Query: 124 DTSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLLL 183
           DTSNDAAWIPCSGCVGC S+ +F   KSSS R L C +PQC                   
Sbjct: 122 DTSNDAAWIPCSGCVGCSSSVLFDPSKSSSSRTLQCEAPQCK------------------ 181

Query: 184 FFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCS-SSACGFNLTYGSSTVAA 243
                                             Q PNPSC+ S +CGFN+TYG ST+ A
Sbjct: 182 ----------------------------------QAPNPSCTVSKSCGFNMTYGGSTIEA 241

Query: 244 DLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSYC 303
            L QD +TLA+D +P YTFGCI KA+G+S+P QGL+GLGRGPLSL+ QSQ+LY+STFSYC
Sbjct: 242 YLTQDTLTLASDVIPNYTFGCINKASGTSLPAQGLMGLGRGPLSLISQSQNLYQSTFSYC 301

Query: 304 LPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSAL 363
           LP+ KS+ FSGSLRLGP  QP RIK TPLL+NPRRSSLYYVNL+ IRVG +IVDIP SAL
Sbjct: 302 LPNSKSSNFSGSLRLGPKNQPIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPTSAL 361

Query: 364 AFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTVPIISPT 423
           AF+ ATGAGTI DSGT +TRLV PAY AVRNEFRRR+ NA  +SLGGFDTCY+  ++ P+
Sbjct: 362 AFDPATGAGTIFDSGTVYTRLVEPAYVAVRNEFRRRVKNANATSLGGFDTCYSGSVVFPS 421

Query: 424 ITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIP 483
           +TFMFAGMNVTLPPDN L+HSSAG  +CLAMAAAP NVNSVLNVIASMQQQNHR+L D+P
Sbjct: 422 VTFMFAGMNVTLPPDNLLIHSSAGNLSCLAMAAAPVNVNSVLNVIASMQQQNHRVLIDVP 425

Query: 484 NSRIGVAREPCS 494
           NSR+G++RE C+
Sbjct: 482 NSRLGISRETCT 425

[Close]

BLAST of Carg01997 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.4e-111
Identity = 230/495 (46.46%), Postives = 302/495 (61.01%), Query Frame = 0


Query: 7   FFLALLLLLSISAESHHHHHHHQHPNCNAAADRSSTLQVFHIFSPCSPFRPSK-PLSWAD 66
           FFL LLL  + +  +       +     AA D S  L +  I + CSPF P+    S  D
Sbjct: 10  FFLTLLLPFTFTTAT-------RDTCATAAPDGSDDLSIIPINAKCSPFAPTHVSASVID 69

Query: 67  NVLQMQAKDQARLQFLSSLVARK---SVAPIASARQLIQSPTFVVRAKIGTPAQTLLMAL 126
            VL M + D  RL +LSSLVA K   +  P+AS  QL     +VVRAK+GTP Q + M L
Sbjct: 70  TVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQL-HIGNYVVRAKLGTPPQLMFMVL 129

Query: 127 DTSNDAAWIPCSGCVGCP-STTVFSSDKSSSFRPLPCRSPQCNQVPTSVLFQINFEFLLL 186
           DTSNDA W+PCSGC GC  ++T F+++ SS++  + C + QC Q                
Sbjct: 130 DTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQA--------------- 189

Query: 187 LFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVPNPSCSSSACGFNLTY-GSSTVA 246
                                   R LT         P+ S   S C FN +Y G S+ +
Sbjct: 190 ------------------------RGLTC--------PSSSPQPSVCSFNQSYGGDSSFS 249

Query: 247 ADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYRSTFSY 306
           A LVQD +TLA D +P ++FGCI  A+G+S+PPQGL+GLGRGP+SL+ Q+ SLY   FSY
Sbjct: 250 ASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSLVSQTTSLYSGVFSY 309

Query: 307 CLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVDIPPSA 366
           CLPSF+S  FSGSL+LG + QPK I+YTPLLRNPRR SLYYVNL  + VG   V + P  
Sbjct: 310 CLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTGVSVGSVQVPVDPVY 369

Query: 367 LAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTV--PII 426
           L F++ +GAGTIIDSGT  TR   P Y A+R+EFR+++  ++ S+LG FDTC++     +
Sbjct: 370 LTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTLGAFDTCFSADNENV 429

Query: 427 SPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHRILF 486
           +P IT     +++ LP +N L+HSSAGT TCL+MA    N N+VLNVIA++QQQN RILF
Sbjct: 430 APKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLRILF 449

Query: 487 DIPNSRIGVAREPCS 494
           D+PNSRIG+A EPC+
Sbjct: 490 DVPNSRIGIAPEPCN 449

[Close]

BLAST of Carg01997 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 173.7 bits (439), Expect = 3.6e-43
Identity = 119/403 (29.53%), Postives = 182/403 (45.16%), Query Frame = 0


Query: 102 SPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGC--PSTTVFSSDKSSSFRPLPCR 161
           S  +  R  +GTPA+ + M LDT +D  W+ C+ C  C   S  +F   KS ++  +PC 
Sbjct: 139 SGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCS 198

Query: 162 SPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVP 221
           SP C ++ ++                  N+R K                           
Sbjct: 199 SPHCRRLDSAG----------------CNTRRK--------------------------- 258

Query: 222 NPSCSSSACGFNLTYG-SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLG 281
                   C + ++YG  S    D   + +T   + V     GC     G  V   GLLG
Sbjct: 259 -------TCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLFVGAAGLLG 318

Query: 282 LGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSS 341
           LG+G LS  GQ+   +   FSYCL    ++    S+  G  A  +  ++TPLL NP+  +
Sbjct: 319 LGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPLLSNPKLDT 378

Query: 342 LYYVNLIAIRV-GRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRR 401
            YYV L+ I V G R+  +  S    +     G IIDSGT+ TRL+ PAY A+R+ F  R
Sbjct: 379 FYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAF--R 438

Query: 402 IGNATIS---SLGGFDTCYTVPIIS----PTITFMFAGMNVTLPPDNFLLHSSAGTTTCL 461
           +G  T+        FDTC+ +  ++    PT+   F G +V+LP  N+L+        C 
Sbjct: 439 VGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLIPVDTNGKFCF 485

Query: 462 AMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPCS 494
           A A         L++I ++QQQ  R+++D+ +SR+G A   C+
Sbjct: 499 AFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Carg01997 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 172.2 bits (435), Expect = 1.1e-42
Identity = 126/406 (31.03%), Postives = 181/406 (44.58%), Query Frame = 0


Query: 102 SPTFVVRAKIGTPAQTLLMALDTSNDAAWIPCSGCVGCPSTT--VFSSDKSSSFRPLPCR 161
           S  + +R  +GTPA  + M LDT +D  W+ CS C  C + T  +F   KS +F  +PC 
Sbjct: 132 SGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCG 191

Query: 162 SPQCNQVPTSVLFQINFEFLLLLFFQLTNSRAKQLLPNSTYRIIKHRTLTARDPFVYQVP 221
           S  C ++  S                                      +T R        
Sbjct: 192 SRLCRRLDDS-----------------------------------SECVTRR-------- 251

Query: 222 NPSCSSSACGFNLTYG-SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLG 281
                S  C + ++YG  S    D   + +T     V     GC     G  V   GLLG
Sbjct: 252 -----SKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDNEGLFVGAAGLLG 311

Query: 282 LGRGPLSLLGQSQSLYRSTFSYCLPSFKSTGFS----GSLRLGPVAQPKRIKYTPLLRNP 341
           LGRG LS   Q+++ Y   FSYCL    S+G S     ++  G  A PK   +TPLL NP
Sbjct: 312 LGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVPKTSVFTPLLTNP 371

Query: 342 RRSSLYYVNLIAIRV-GRRIVDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNE 401
           +  + YY+ L+ I V G R+  +  S    ++    G IIDSGT+ TRL  PAY A+R+ 
Sbjct: 372 KLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTRLTQPAYVALRDA 431

Query: 402 FR---RRIGNATISSLGGFDTCYTV----PIISPTITFMFAGMNVTLPPDNFLLHSSAGT 461
           FR    ++  A   SL  FDTC+ +     +  PT+ F F G  V+LP  N+L+  +   
Sbjct: 432 FRLGATKLKRAPSYSL--FDTCFDLSGMTTVKVPTVVFHFGGGEVSLPASNYLIPVNTEG 483

Query: 462 TTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRIGVAREPC 493
             C A A    +    L++I ++QQQ  R+ +D+  SR+G     C
Sbjct: 492 RFCFAFAGTMGS----LSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of Carg01997 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031923.1	5.6e-280	100.00	Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6601125.1	1.6e-242	89.45	Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023526864.1	4.8e-239	88.46	aspartyl protease AED3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022957241.1	1.0e-236	88.03	aspartyl protease AED3 [Cucurbita moschata]	[more]
XP_022988941.1	1.1e-232	87.04	aspartyl protease AED3-like [Cucurbita maxima]	[more]

BLAST of Carg01997 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04496	2.0e-110	46.46	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]
Q6F4N5	6.4e-93	43.16	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]
Q9LNJ3	5.1e-42	29.53	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	8.5e-37	29.00	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LEW3	1.9e-36	27.94	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]

BLAST of Carg01997 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GYP2	4.9e-237	88.03	aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1	[more]
A0A6J1JIM3	5.6e-233	87.04	aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=...	[more]
A0A5A7SU62	5.8e-214	80.89	Aspartyl protease AED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4...	[more]
A0A1S3BGB2	5.8e-214	80.89	aspartyl protease AED3 OS=Cucumis melo OX=3656 GN=LOC103489519 PE=4 SV=1	[more]
A0A0A0KTR5	3.8e-213	80.08	Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=...	[more]

BLAST of Carg01997 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07030.1	1.1e-159	60.00	Eukaryotic aspartyl protease family protein	[more]
AT3G54400.1	1.0e-154	59.15	Eukaryotic aspartyl protease family protein	[more]
AT1G09750.1	1.4e-111	46.46	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	3.6e-43	29.53	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.1e-42	31.03	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 340..486 e-value: 1.4E-27 score: 96.5
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 105..165 e-value: 7.5E-12 score: 45.8 coord: 197..317 e-value: 2.5E-19 score: 70.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 318..493 e-value: 7.2E-46 score: 158.1
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 185..317 e-value: 5.5E-17 score: 64.2 coord: 82..171 e-value: 2.0E-13 score: 52.6
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 104..492
None	No IPR available	PANTHER	PTHR13683:SF798	ASPARTYL PROTEASE AED3-LIKE	coord: 9..169 coord: 205..493
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 9..169 coord: 205..493
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 105..488 score: 35.762848

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01997-RA	Carg01997-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity