Carg01884 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01884
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectinesterase
LocationCarg_Chr04: 8807358 .. 8810654 (-)
RNA-Seq ExpressionCarg01884
SyntenyCarg01884
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTCAGTTCCCTTCATTTTCCTTCTCTTCCTCTCTCTCGGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGTTCGATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAAGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTCGATGAGGAACTGGTGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTAACGGCACCGCTGTTGAGCGCGGGGAAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAAGCAGAAGGGAGACGGCAAGGGAGGGAAATTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTAATCAAGGTACGATTCGATATGGCGCCGTTGTTATTTGATTTCTGTTTGTCTGTTCGTTCGGTTACACGGGCCATTACATTTGCTTTATTCAATGCATCAAAAAATTGGGCGAAAATAAGTTTTTGTTTGGATGTCGCCACTAAAATATAAAATTAATCTAATAAATAATAATGTTATATTTTTTTCAAAGTAATAGTAAAACAATAAATCATTAACAAATAAGCTTCTTCTTTAAGAATTAATCTTACAAATAAAATTGTTATTAAAATGTGTAATCACCCTTGTAATTAATGCTTCAAAAGTGCATTAAATTCTCCCATAATGACACTATTACTGAGCCGAAATCACAATTGATGGTAGCATTAACGGGAATTAGTGAGGGAAAAAACAGTTGAATATGTTTAAGTATTCATAGTTATATAATTTTAAAACTATGCTTTGATTTAATTTAATGCATATTTAATTGATTTCTGATTTCTTTCATATTACTTGTGAATTACTTACATTGGAATATCATCTTTATACAAAGTGAGGAATTTAACTTTTCATAAAGAACTATAGTTTAATATTTGAATTTTTACCAATATGATCAGTAAGAACATATATTAAATAAATAACAAAAATGTTTTTTAGTGGAAAAAAAGAGTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTGTTCATTAAATTTATGTTAAATACGTTGATAATTTTGTTGGTGTCACGGGAGAGATTCTAGGATTCCCAAGCTCCACCAAATGCATGTTACAAATTCAGATGTGGATTCTCCTCATAATTTTTAATTTGCCTTTTTTATAATTGTTTTATTCCTATCATTTGCCTTTTATTTTATATATATATATATATGTATGTATATATATATGTATGTATGTATGTATGTATGTATGTTTATGTATATGTATATGTATATGTGTGTATATATATACATATATATATAGATATGTATGTATGTGTATATGTATATGTATATGAATATGTATGTGGGCCGGCAACCCGACCAGCTAATAGCAACTCCAAACTCCGATGTCGTAGGTTCAAATTATACAGAATGTGATTCGATTGTTGTAATCGAACGATTTCCTTAAGTTTTGTCCCGTGTAAAAAATCCTAAAACAAGTCTTGATCTATACAAAGTGCACTAGGTTTTTGTCTTTGGGTACTACGAACCCTCTGCCATCTATTGCTGTAGAGCCGTTTCATGAGCAGGGGCTAAGACCAGAAAGAAGAGGCTTTCCTGTGGACTGGGCGTGGGCGTGGGCTAAGCTAGAAGAGTTGATATATATGTTGAAACTAATCTTGGAATTTAAGACAATCTAAGGAGCAATTGTTCCTTCTTTTTTTTTTTTCTCAAGTTCTTTTAATATAAATTAGTTGCTGATTTTTTGAGGTAATTGTATCTTTTTTTTCCTACGAATTTACAATGATCTTAAGTGTTTGAATGAACAGAGACTACATACGATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGAAAGGAAGCGCTTGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATCGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATACGTTATCGTCCCCAAGTACAAAACAAACATAATGCTCATTGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTCGTCGATGGTTGGACGACTTACAATTCCTCTACCTTTAGTAAGCAATTCACAAATCCATAACTTATTCGTTTACAATATTGTGGTATCAAAATTATTCCGAAATTGGTAGCTGAATGTTTGGGATATATGTTGGCAGCTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCATGAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTATTCCAGCAATGTAATCTATACGCTAGAAAGCCACTCCCTAACCAAAAGAACGCCTTCACAGCGCAGGGCCGAACCGATCCGAACCAAAGCACGGGCATCTCAATCCACAACTGCACCATCAAGGCTGCCCCAGACTGGGTGATGGATTTGAATGAGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGTTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGAGCAGGAGCTAACACGAGCTCGAGGGTCAACTGGGCTGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACCATGGGAGATACTTGGCTGCCCTATACAGATATCCCCTTCTCTCGAGGTTTGCTATGA

mRNA sequence

ATGAACTCAGTTCCCTTCATTTTCCTTCTCTTCCTCTCTCTCGGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGTTCGATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAAGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTCGATGAGGAACTGGTGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTAACGGCACCGCTGTTGAGCGCGGGGAAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAAGCAGAAGGGAGACGGCAAGGGAGGGAAATTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTAATCAAGAGACTACATACGATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGAAAGGAAGCGCTTGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATCGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATACGTTATCGTCCCCAAGTACAAAACAAACATAATGCTCATTGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTCGTCGATGGTTGGACGACTTACAATTCCTCTACCTTTACTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCATGAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTATTCCAGCAATGTAATCTATACGCTAGAAAGCCACTCCCTAACCAAAAGAACGCCTTCACAGCGCAGGGCCGAACCGATCCGAACCAAAGCACGGGCATCTCAATCCACAACTGCACCATCAAGGCTGCCCCAGACTGGGTGATGGATTTGAATGAGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGTTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGAGCAGGAGCTAACACGAGCTCGAGGGTCAACTGGGCTGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACCATGGGAGATACTTGGCTGCCCTATACAGATATCCCCTTCTCTCGAGGTTTGCTATGA

Coding sequence (CDS)

ATGAACTCAGTTCCCTTCATTTTCCTTCTCTTCCTCTCTCTCGGCCTCGCCGGAGCTTCCGATTCTCCTCCGCCGTCCGCCGTCCTCTGTAAATCCACTCTATATCCTAAGCTTTGCCGTTCGATTCTCTCCTCCATCCGATTCTCCCCCTCCGATCCATACGGCTACGGAAAATTCTCCGTCAAGAAGTGTCTCAAACAGGCGATCAAAATGTCGAGCGTTATCGGCGAGTATCTCGACCGAGGAGGCGATTCGAGGTTGAATCGGCAGGAGGTTGGCGCTCTGGAAGACTGCAGAGAGCTTTCCGATTCGAATGTGGAGTTTCTCCAGTCGATTGATGGCGTATTGGAGGCAGCGGAGGGCGTCGATGAGGAACTGGTGGAGCGTATTGAGAGTATTTTGAGCGCGATCGTGACGAACGGCCAGACTTGTATCGACGGATTGGTGGAGTCGAGAAGTAGTTTGGGGAATGCGCTAACGGCACCGCTGTTGAGCGCGGGGAAGCTGTACAGTGTATCGTTAGGACTGGTTAAGAACTCGCTGTCGCGGCGGTGGAAGAAGCGGAAGCAGAAGGGAGACGGCAAGGGAGGGAAATTCCCGGCGGCTGCGCAGAGCAGAGAGCCATTGAACACGCTAATCAAGAGACTACATACGATGGGTTCGTGCAACCAATCCACAAAGTGTTCGGAAAGGAAGCGCTTGTTGACTGATTTGGGCACCACAGGAATTTTGATCAACAACACTGTGGTTGTTAGCTCAACCGGTGCTGACAACTTCACATCAATCGGAGATGCAATTTCATTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGACAAGGATACTACGACGAATACGTTATCGTCCCCAAGTACAAAACAAACATAATGCTCATTGGAGATGGCATTAACCGAACCGTCATCACGGGGAACCATAACGTCGTCGATGGTTGGACGACTTACAATTCCTCTACCTTTACTGTTTATGGGGATGGATTTGTTGCAATAGACATGACATTCAGGAACACAGCAGGAGCAGAGAAGCACCAAGCAGTGGCAGTGAGGAACAGTGCAGATCTCTCAACATTCTACCGCTGTAGCTTTGAGGGATATCAAGACACTCTTTATGTACATTCCATGAGGCAATTCTACAGGGAATGCGACGTTTATGGCACCGTCGACTTCATTTTTGGCAACGCTGCTGCAGTATTCCAGCAATGTAATCTATACGCTAGAAAGCCACTCCCTAACCAAAAGAACGCCTTCACAGCGCAGGGCCGAACCGATCCGAACCAAAGCACGGGCATCTCAATCCACAACTGCACCATCAAGGCTGCCCCAGACTGGGTGATGGATTTGAATGAGACGACGAACTATCTCGGGCGGCCATGGAAGCCGTTCTCAAGGACGGTGTATATGCAGTCTTACATTGGGGATTTGATCAGCCCAGTGGGGTGGTTGGAGTGGAATGGAACGGTGGGATTGGACACATTGTATTATGGGGAGTTTGAGAATTATGGAGCAGGAGCTAACACGAGCTCGAGGGTCAACTGGGCTGGCCATAGCTTGATGAATATCACAGAGGCTATGAATTTTACTGTCTATAACTTCACCATGGGAGATACTTGGCTGCCCTATACAGATATCCCCTTCTCTCGAGGTTTGCTATGA

Protein sequence

MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL
Homology
BLAST of Carg01884 vs. NCBI nr
Match: KAG7032035.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240
           LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT
Sbjct: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQ 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQ
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQ 480

Query: 481 SYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTV 540
           SYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTV
Sbjct: 481 SYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTV 540

Query: 541 YNFTMGDTWLPYTDIPFSRGLL 563
           YNFTMGDTWLPYTDIPFSRGLL
Sbjct: 541 YNFTMGDTWLPYTDIPFSRGLL 562

BLAST of Carg01884 vs. NCBI nr
Match: KAG6601245.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 554/566 (97.88%), Postives = 556/566 (98.23%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSVPFIFLLFLSLGLAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240
           LSRRWKKRKQKG G+GGKFPAAAQSREPLNTLIK LHTMGSCNQSTKCS RKRLLTDLGT
Sbjct: 181 LSRRWKKRKQKGGGEGGKFPAAAQSREPLNTLIKGLHTMGSCNQSTKCSGRKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLN----ETTNYLGRPWKPFSRT 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAP+WVMDLN     T NYLGRPWKPFSRT
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDLNATKTTTMNYLGRPWKPFSRT 480

Query: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAM 540
           VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAM 540

Query: 541 NFTVYNFTMGDTWLPYTDIPFSRGLL 563
           NFTVYNFTMGDTWLPYTDIPFSRGLL
Sbjct: 541 NFTVYNFTMGDTWLPYTDIPFSRGLL 566

BLAST of Carg01884 vs. NCBI nr
Match: XP_023549932.1 (probable pectinesterase/pectinesterase inhibitor 25 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 547/566 (96.64%), Postives = 552/566 (97.53%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLLFLSLGLAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSIPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VKKC+KQAIKMSSVIGEYL+RGGDSRLNRQE GALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCIKQAIKMSSVIGEYLNRGGDSRLNRQEFGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240
           LSRRWKKRKQKGDG+GG FPAAAQSREPLNTLIK LHTMGSCNQSTKCS RKRLLTDLGT
Sbjct: 181 LSRRWKKRKQKGDGEGGNFPAAAQSREPLNTLIKGLHTMGSCNQSTKCSGRKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETT----NYLGRPWKPFSRT 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAP+WVMD N TT    NYLGRPWKPFSRT
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDSNATTTTTMNYLGRPWKPFSRT 480

Query: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAM 540
           VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANT SRVNW GHSLMNITEAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTRSRVNWGGHSLMNITEAM 540

Query: 541 NFTVYNFTMGDTWLPYTDIPFSRGLL 563
           NFTVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 566

BLAST of Carg01884 vs. NCBI nr
Match: XP_023549916.1 (probable pectinesterase/pectinesterase inhibitor 25 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1094.3 bits (2829), Expect = 0.0e+00
Identity = 547/571 (95.80%), Postives = 552/571 (96.67%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLLFLSLGLAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSIPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VKKC+KQAIKMSSVIGEYL+RGGDSRLNRQE GALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCIKQAIKMSSVIGEYLNRGGDSRLNRQEFGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKR-----LHTMGSCNQSTKCSERKRLL 240
           LSRRWKKRKQKGDG+GG FPAAAQSREPLNTLIK      LHTMGSCNQSTKCS RKRLL
Sbjct: 181 LSRRWKKRKQKGDGEGGNFPAAAQSREPLNTLIKSLNEQGLHTMGSCNQSTKCSGRKRLL 240

Query: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV 300
           TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV
Sbjct: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV 300

Query: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360
           PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH
Sbjct: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360

Query: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420
           QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL
Sbjct: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420

Query: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETT----NYLGRPWK 480
           YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAP+WVMD N TT    NYLGRPWK
Sbjct: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDSNATTTTTMNYLGRPWK 480

Query: 481 PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMN 540
           PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANT SRVNW GHSLMN
Sbjct: 481 PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTRSRVNWGGHSLMN 540

Query: 541 ITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL 563
           ITEAMNFTVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 ITEAMNFTVYNFTMGDTWLPYTDIPFSGGLL 571

BLAST of Carg01884 vs. NCBI nr
Match: XP_023549924.1 (probable pectinesterase/pectinesterase inhibitor 25 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 546/571 (95.62%), Postives = 551/571 (96.50%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLLFLSLGLAGASDSPPPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSIPFIFLLFLSLGLAGASDSPPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VKKC+KQAIKMSSVIGEYL+RGGDSRLNRQE GALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCIKQAIKMSSVIGEYLNRGGDSRLNRQEFGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKR-----LHTMGSCNQSTKCSERKRLL 240
           LSRRWKKRKQKGDG+GG FPAAAQSREPLNTLIK      LHTMGSCNQSTKCS RKRLL
Sbjct: 181 LSRRWKKRKQKGDGEGGNFPAAAQSREPLNTLIKSLNEQGLHTMGSCNQSTKCSGRKRLL 240

Query: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV 300
           TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV
Sbjct: 241 TDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIV 300

Query: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360
           PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH
Sbjct: 301 PKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKH 360

Query: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420
           QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL
Sbjct: 361 QAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNL 420

Query: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETT----NYLGRPWK 480
           YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAP+WVMD N TT    NYLGRPWK
Sbjct: 421 YARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDSNATTTTTMNYLGRPWK 480

Query: 481 PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMN 540
           PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANT SRVNW GHSLMN
Sbjct: 481 PFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTRSRVNWGGHSLMN 540

Query: 541 ITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL 563
           ITEAMNFTVYNFTMGDTWLPYTDIPFS G L
Sbjct: 541 ITEAMNFTVYNFTMGDTWLPYTDIPFSGGRL 571

BLAST of Carg01884 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 2.0e-191
Identity = 328/549 (59.74%), Postives = 418/549 (76.14%), Query Frame = 0

Query: 20  SDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVIGEYL 79
           S S  PS + CKST YPKLCR+IL++++ SPSDPY YGKF++K+CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERIESILSAI 139
            R          +E+GA+ DC ELS+ +V +L+++   L+ A+ +   LVE + S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSRRWKKRK-QKGDGKG 199
           VTN QTC+DGLVE++S    A+ +P+ +  +LYS+SLGLV ++L+R  K+ K  KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTT---GILINNTVVVSS 259
           G     +  REPL TLIK L    +C+    C +  R L +LG T    IL++  V+V  
Sbjct: 254 G---GNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 260 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTV 319
             +DNFT+I DAI+ APNN+ P+DGYFVIY R+G Y+EY++VP  K N+ML+GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 320 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 379
           ITGNHNVVDGWTTYN S+F V G+ F+A+D+TFRNTAG EKHQAVA+RN+A+ S+FYRCS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 380 FEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 439
           FEGYQDTLYVHS+RQFYRECD+YGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 440 TDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 499
            DPNQ+TGISI NCTIKAAPD   +      +LGRPWKP+SRTV+MQSYI D++ PVGWL
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWL 553

Query: 500 EWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYT 559
           EWNGT+GLDT+YYGE+ N+G GANT+ RV W G++L+N+ EAMNFTVYNFTMGDTWLP T
Sbjct: 554 EWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQT 613

Query: 560 DIPFSRGLL 563
           DIPF  GLL
Sbjct: 614 DIPFYGGLL 616

BLAST of Carg01884 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 1.1e-189
Identity = 329/549 (59.93%), Postives = 412/549 (75.05%), Query Frame = 0

Query: 22  SPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVIGEYLDR 81
           SP P  + CKST YPKLCR+ILS+++ SPSDPY YGKF++K+CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERIESILSAIVT 141
                     +EV A+ DC EL++ +VE+L+++   L+AAE +   LV+R+ S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSR---RWKKRKQKGDGKG 201
           N QTC+DGLV+++S    A+  PL +  +LYSVSLGLV ++L+R   R+K  K K  G G
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 GKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTT---GILINNTVVVSS 261
            K       REPL TLIK L    +C++   C +  R L +LG T    IL+   V V  
Sbjct: 260 NK-----PVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGP 319

Query: 262 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTV 321
              DNF +I +A++ APN++ P+ GYFVIY R G Y+EYV++   K NIMLIGDGIN+T+
Sbjct: 320 YETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTI 379

Query: 322 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 381
           I+GNH+ +DGWTTYNSSTF V GD FVA+D+TFRNTAG EKHQAVAVRN+AD STFYRCS
Sbjct: 380 ISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCS 439

Query: 382 FEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 441
           FEGYQDTLYVHS+RQFYRECD+YGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GR
Sbjct: 440 FEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGR 499

Query: 442 TDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 501
           TDPNQ TGISI NCTI AAPD   D   T  +LGRPWKP+SRTVY+QSYI D++ PVGWL
Sbjct: 500 TDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWL 559

Query: 502 EWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYT 561
           EWNGT GLDT+ YGE++N+G GA+TS RV W+G+SL+N+ +AMNFTVYNFT+GDTWLP T
Sbjct: 560 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQT 619

Query: 562 DIPFSRGLL 563
           DIPF  GLL
Sbjct: 620 DIPFYGGLL 621

BLAST of Carg01884 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 505.4 bits (1300), Expect = 8.5e-142
Identity = 277/573 (48.34%), Postives = 369/573 (64.40%), Query Frame = 0

Query: 6   FIFLLFLSL-GLAGASDSPPP---SAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 65
           F+  LFLSL  L  AS +  P   S+ +CK+T  PK C+S+    + S  D   YG+FS+
Sbjct: 7   FLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSL 66

Query: 66  KKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQS---IDGVLEA 125
           +K L Q+ K +  I  YL R  ++ L++  VGAL+DCR L+    ++L +      +  +
Sbjct: 67  RKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTS 126

Query: 126 AEGVDEELVERIESILSAIVTNGQTCIDGLVESRSS---LGNALTAPLLSAGKLYSVSLG 185
           ++ +     + I+++LSA +TN QTC+DG+  + SS   + N +  PL++  KL+SVSL 
Sbjct: 127 SKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLA 186

Query: 186 LVKNSLSRRW--KKRKQKGDGKGGKFPAAAQSREPL----NTLIKRLHTMGSCNQSTKCS 245
           L     ++ W  KK+KQ                +P     N  +    T  +       S
Sbjct: 187 L----FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLS 246

Query: 246 ERKRLLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYY 305
            RK    D     +L+++ V V+  G  NFT+I +A++ APN +    GYFVIYV  G Y
Sbjct: 247 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 306

Query: 306 DEYVIVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNT 365
           +E V++ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA++MTFRNT
Sbjct: 307 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 366

Query: 366 AGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAV 425
           AG EKHQAVA+R+SADLS FY CSFE YQDTLY HS+RQFYRECD+YGTVDFIFGNAA V
Sbjct: 367 AGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV 426

Query: 426 FQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRP 485
           FQ CNLY R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTIK A D V        YLGRP
Sbjct: 427 FQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRP 486

Query: 486 WKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSL 545
           WK +SRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G+G++T+ RV W G+ +
Sbjct: 487 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 546

Query: 546 MNITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL 563
           +N T+A NFTV NF +GD W+  + +P+  GLL
Sbjct: 547 INSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Carg01884 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 8.8e-131
Identity = 265/582 (45.53%), Postives = 360/582 (61.86%), Query Frame = 0

Query: 7   IFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLK 66
           I L F    +  +S +   S+ +CK+T  PK C+S+      S  +   YG FS++K L 
Sbjct: 9   ITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSIRKSLS 68

Query: 67  QAIKMSSVIGEYLDRGGDSRLNRQEV-GALEDCRELSDSNVEF-LQSIDGVLEAA----- 126
           Q+ K    +  Y+ R  ++ L++  V  AL+DCR L+   +++ L S + V + +     
Sbjct: 69  QSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSF 128

Query: 127 EGVDEELVERIESILSAIVTNGQTCIDGLVESRS-----SLGNALTAPLLSAGKLYSVSL 186
           + +     + I+++LSA +TN QTC++GL  + S     ++   +  PL++  KL  VSL
Sbjct: 129 KPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSL 188

Query: 187 GLVKNSLSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGS----CNQSTKC-- 246
            L     ++ W  +K+K  G     P +  S    +T   RL   G+      + TK   
Sbjct: 189 AL----FTKGWVPKKKKRAGFAWAQPRSGSS---THTKPFRLFRNGALPLKMTEKTKAVY 248

Query: 247 -SERKRLLTDLGTTG-------ILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYF 306
            S  +R L D  + G       +LI++ V VS  G  NFT+I  A++ APNN+    G+F
Sbjct: 249 ESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFF 308

Query: 307 VIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFV 366
           +IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V    FV
Sbjct: 309 LIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFV 368

Query: 367 AIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVD 426
           A+++TFRNTAG EKHQAVA+R+ AD S FY CSFE YQDTLY HS+RQFYRECDVYGTVD
Sbjct: 369 AVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVD 428

Query: 427 FIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLN 486
           FIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTIK A D V    
Sbjct: 429 FIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNY 488

Query: 487 ETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSS 546
               YLGRPWK +SRTVYMQSYI   + PVGW EWNG   L TLYY E+ N G G+NT++
Sbjct: 489 TVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTN 548

Query: 547 RVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL 563
           RV W G+ ++N T+A NFTV    +   W+  T +P++ GL+
Sbjct: 549 RVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Carg01884 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 251/558 (44.98%), Postives = 340/558 (60.93%), Query Frame = 0

Query: 11  FLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIK 70
           F+S  +    ++  PS + C+    P  CRS+L +    P D Y YG+ S+++ L +A +
Sbjct: 21  FISAQIPAIGNATSPSNI-CRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARR 80

Query: 71  MSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERI 130
             S+I   LDR G     +  VGALEDC+ L+   +++L S     ++ + +     E +
Sbjct: 81  FISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDV 140

Query: 131 ESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSRRWKKRKQ 190
            + LSA +TN QTC++GL  + S   N L+  L +  KLY VSL L     S+ W  R+Q
Sbjct: 141 HTFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQ 200

Query: 191 KG------DGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTTGIL 250
           +         +  KF      + PL  + +R   +         + RK L +D     + 
Sbjct: 201 RSRPIWQPQARFKKFFGFRNGKLPLK-MTERARAV-----YNTVTRRKLLQSD--ADAVQ 260

Query: 251 INNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIML 310
           +++ V V   G  NFT+I  AI+ APN +   +GYF+IYV  G Y+EYV VPK K  +M+
Sbjct: 261 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 320

Query: 311 IGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSA 370
           IGDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAG  K QAVA+R+  
Sbjct: 321 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 380

Query: 371 DLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQ 430
           DLS FY CSFE YQDTLY HS+RQFYRECDVYGTVDFIFGNAA V Q CNLY R+P   Q
Sbjct: 381 DLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQ 440

Query: 431 KNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIG 490
            N  TAQGRTDPNQ+TG +IH CTI+ A D          YLGRPWK +SRTV MQ+YI 
Sbjct: 441 SNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYID 500

Query: 491 DLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFT 550
             + P GW  W+G   L TLYY E+ N G G++T++RV W G+ ++N T+A NFTV NF 
Sbjct: 501 GFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFL 559

Query: 551 MGDTWLPYTDIPFSRGLL 563
           +G+ W+  T +PF  GL+
Sbjct: 561 VGEGWIGQTGVPFVGGLI 559

BLAST of Carg01884 vs. ExPASy TrEMBL
Match: A0A6J1GYC3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 544/566 (96.11%), Postives = 550/566 (97.17%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNSVPFIFLLFLSLGLAGASDS PPSA LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSAPPSAFLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VKKCLKQAIKMSSVIGEYL+RGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VKKCLKQAIKMSSVIGEYLNRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNAL+APLLSAG+LYSVSLGLVKNS
Sbjct: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240
           LSRRWKKR+QKGDG+GG FPAAAQSREPLNTLIK LH M SCNQSTKC  RKRLLTDLGT
Sbjct: 181 LSRRWKKRRQKGDGEGGNFPAAAQSREPLNTLIKGLH-MSSCNQSTKCWGRKRLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSM QDGYFVIYVRQGYYDEYVIVPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMLQDGYFVIYVRQGYYDEYVIVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETT----NYLGRPWKPFSRT 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAP+WVMDLN TT    NYLGRPWKPFSRT
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPEWVMDLNATTTTTMNYLGRPWKPFSRT 480

Query: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAM 540
           VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNW GHSLMNITEAM
Sbjct: 481 VYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWGGHSLMNITEAM 540

Query: 541 NFTVYNFTMGDTWLPYTDIPFSRGLL 563
           NFTVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 NFTVYNFTMGDTWLPYTDIPFSGGLL 565

BLAST of Carg01884 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 537/563 (95.38%), Postives = 548/563 (97.34%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS PFIFL  LSL LAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           V+KCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE
Sbjct: 61  VRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEEL+ERIESILSAIVTNGQTCIDGLVESRSSLGNAL+APLLSAG+LYSVSLGLVKNS
Sbjct: 121 GVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240
           LSRRWKKR QKGD +GG FPAAAQSREPLNTLIK LH+MGSCNQSTKCS RKR+LTDLGT
Sbjct: 181 LSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300
           TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYV+VPKYKT
Sbjct: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNE-TTNYLGRPWKPFSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI AAP+WVMDLNE TTNYLGRPWKPFSRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTITAAPNWVMDLNETTTNYLGRPWKPFSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFT 540
           QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYG GANTS RV+W GHSLMNITEAMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGPGANTSLRVDWDGHSLMNITEAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFSRGLL 563
           VYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 VYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Carg01884 vs. ExPASy TrEMBL
Match: A0A1S3BFR9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 4.2e-285
Identity = 488/564 (86.52%), Postives = 523/564 (92.73%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLL LSL  AGAS SPP S  LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPSSTALCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VK+C+KQAIKMS+VIG+YLDRG DSRLNR+E GAL DCRELSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQAIKMSTVIGDYLDRGRDSRLNRREAGALSDCRELSDLNVEFLRSIESVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVER+ES LSAIVTNGQTC+DGLVESRSSLG AL+APLLSAG+LYSVSLGLV N+
Sbjct: 121 GVDEELVERVESTLSAIVTNGQTCVDGLVESRSSLGKALSAPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSC-NQSTKCSERKRLLTDLG 240
           +SRRWKKR++KG G GG  P   +SRE LNTLIK LH M  C N STKCS RKRLLTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREALNTLIKGLHKMEPCNNHSTKCSGRKRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYK 300
           ++GILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR+GYY+EYV+VPK+K
Sbjct: 241 SSGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVREGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLN-ETTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTIKAAPDWVMD N  TTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYG GANTS RVNW G SLMN+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLMNLTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSRGLL 563
           TVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Carg01884 vs. ExPASy TrEMBL
Match: A0A0A0KUN8 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1)

HSP 1 Score: 987.3 bits (2551), Expect = 2.7e-284
Identity = 485/564 (85.99%), Postives = 524/564 (92.91%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+PFIFLL LSL  AGAS SPPPS  LCKSTLYPKLCRSILS+IRFSPSDPYGYGKFS
Sbjct: 1   MNSLPFIFLLLLSLNFAGASVSPPPSTALCKSTLYPKLCRSILSTIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VK+C+KQA KMS+VIG+YL+RG DSRLNR E GAL DCR+LSD NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCIKQATKMSTVIGDYLNRGRDSRLNRPEAGALSDCRDLSDLNVEFLRSIERVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDEELVER+ESILSAIVTNGQTCIDGLVESRSSLGNAL+ PLLSAG+LYSVSLGLV N+
Sbjct: 121 GVDEELVERVESILSAIVTNGQTCIDGLVESRSSLGNALSGPLLSAGELYSVSLGLVSNA 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSC-NQSTKCSERKRLLTDLG 240
           +SRRWKKR++KG G GG  P   +SREPL+TLIK LH M  C NQSTKC  R+RLLTDLG
Sbjct: 181 MSRRWKKRREKGGGNGG-VPGGGRSREPLDTLIKGLHKMEPCNNQSTKCLGRQRLLTDLG 240

Query: 241 TTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYK 300
           +TGILINNTVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYV++GYY+EYV+VPK+K
Sbjct: 241 STGILINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVKEGYYEEYVVVPKFK 300

Query: 301 TNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVA 360
           TNIMLIGDGINRT+ITGNHNVVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVA
Sbjct: 301 TNIMLIGDGINRTIITGNHNVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 VRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARK 420
           +RNSADLSTFYRCSFEGYQDTLYVHS+RQFYRECD+YGTVDFIFGNAAAVFQQCNLYARK
Sbjct: 361 LRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARK 420

Query: 421 PLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLN-ETTNYLGRPWKPFSRTVY 480
           PLPNQKNAFTAQGRTDPNQ+TGISIHNCTIKAAPDWVMD N  TTNYLGRPWK +SRTVY
Sbjct: 421 PLPNQKNAFTAQGRTDPNQNTGISIHNCTIKAAPDWVMDSNTTTTNYLGRPWKQYSRTVY 480

Query: 481 MQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNF 540
           MQSYIGDLISPVGWLEWNGTVGL+TLYYGE+ENYG GANTS RVNW G SL+N+T+AMNF
Sbjct: 481 MQSYIGDLISPVGWLEWNGTVGLETLYYGEYENYGPGANTSLRVNWPGFSLLNVTQAMNF 540

Query: 541 TVYNFTMGDTWLPYTDIPFSRGLL 563
           TVYNFTMGDTWLPYTDIPFS GLL
Sbjct: 541 TVYNFTMGDTWLPYTDIPFSGGLL 563

BLAST of Carg01884 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 3.1e-280
Identity = 481/563 (85.44%), Postives = 519/563 (92.18%), Query Frame = 0

Query: 1   MNSVPFIFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFS 60
           MNS+P+IFLL LSL LAGASDSPPPSA LCK+TLYPKLC SILSSIRFSPSDPYGYGKFS
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPPSAALCKTTLYPKLCHSILSSIRFSPSDPYGYGKFS 60

Query: 61  VKKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAE 120
           VK+CLKQAIKMSSVIG YL RGG SRLN++E GAL+DCREL+D NVEFL+SI+ VLEAAE
Sbjct: 61  VKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVLEAAE 120

Query: 121 GVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNS 180
           GVDE++VER+ESILSAIVTNGQTCIDGLVES+SSLG+AL+APL +AG+LYSVSLGLVKNS
Sbjct: 121 GVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGLVKNS 180

Query: 181 LSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGT 240
           LSRRWKKR+Q   G GG       +REPL TLIK L  MGSCNQST CS R+RLLTDLGT
Sbjct: 181 LSRRWKKRRQSFPGGGG-------NREPLITLIKGLRKMGSCNQSTICSGRERLLTDLGT 240

Query: 241 TGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKT 300
           TGI INNTVVVSSTGADNFTSIGDAI+F+PNNSMP+DGYFVIYVR+GYY+EYV+VPKYKT
Sbjct: 241 TGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKYKT 300

Query: 301 NIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAV 360
           NIMLIGDGINRTVITGNH+VVDGWTTYNSSTFTV GDGFVAID+TFRNTAG EKHQAVAV
Sbjct: 301 NIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAVAV 360

Query: 361 RNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKP 420
           RNSADLSTFYRCSFEGYQDTLYVHS RQFYRECD+YGTVDFIFGNAAAVFQQCNLYARKP
Sbjct: 361 RNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYARKP 420

Query: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNET-TNYLGRPWKPFSRTVYM 480
           LPNQKNAFTAQGRTDPNQSTGISIHNCTI+AAPDW MD N T  NYLGRPWK +SRTVYM
Sbjct: 421 LPNQKNAFTAQGRTDPNQSTGISIHNCTIRAAPDWAMDSNATIANYLGRPWKQYSRTVYM 480

Query: 481 QSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFT 540
           QSYIGDLISPVGWLEWNGT GL+TLYYGEFEN+G GANTS RVNW G+SLMN T+AMNFT
Sbjct: 481 QSYIGDLISPVGWLEWNGTAGLETLYYGEFENHGPGANTSMRVNWPGYSLMNATQAMNFT 540

Query: 541 VYNFTMGDTWLPYTDIPFSRGLL 563
           VYNFTMGDTWLPYTDIPF+ GL+
Sbjct: 541 VYNFTMGDTWLPYTDIPFNGGLV 556

BLAST of Carg01884 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 670.2 bits (1728), Expect = 1.4e-192
Identity = 328/549 (59.74%), Postives = 418/549 (76.14%), Query Frame = 0

Query: 20  SDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVIGEYL 79
           S S  PS + CKST YPKLCR+IL++++ SPSDPY YGKF++K+CLKQA ++S VI  Y 
Sbjct: 74  SQSQSPS-LACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYA 133

Query: 80  DR--GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERIESILSAI 139
            R          +E+GA+ DC ELS+ +V +L+++   L+ A+ +   LVE + S+LS +
Sbjct: 134 RRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHVNSLLSGV 193

Query: 140 VTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSRRWKKRK-QKGDGKG 199
           VTN QTC+DGLVE++S    A+ +P+ +  +LYS+SLGLV ++L+R  K+ K  KG   G
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKILG 253

Query: 200 GKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTT---GILINNTVVVSS 259
           G     +  REPL TLIK L    +C+    C +  R L +LG T    IL++  V+V  
Sbjct: 254 G---GNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGP 313

Query: 260 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTV 319
             +DNFT+I DAI+ APNN+ P+DGYFVIY R+G Y+EY++VP  K N+ML+GDGIN+T+
Sbjct: 314 FKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTI 373

Query: 320 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 379
           ITGNHNVVDGWTTYN S+F V G+ F+A+D+TFRNTAG EKHQAVA+RN+A+ S+FYRCS
Sbjct: 374 ITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCS 433

Query: 380 FEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 439
           FEGYQDTLYVHS+RQFYRECD+YGTVDFIFGNAAA+FQ CN+YARKP+  QKNA TA GR
Sbjct: 434 FEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGR 493

Query: 440 TDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 499
            DPNQ+TGISI NCTIKAAPD   +      +LGRPWKP+SRTV+MQSYI D++ PVGWL
Sbjct: 494 LDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWL 553

Query: 500 EWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYT 559
           EWNGT+GLDT+YYGE+ N+G GANT+ RV W G++L+N+ EAMNFTVYNFTMGDTWLP T
Sbjct: 554 EWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQT 613

Query: 560 DIPFSRGLL 563
           DIPF  GLL
Sbjct: 614 DIPFYGGLL 616

BLAST of Carg01884 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 664.5 bits (1713), Expect = 7.8e-191
Identity = 329/549 (59.93%), Postives = 412/549 (75.05%), Query Frame = 0

Query: 22  SPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIKMSSVIGEYLDR 81
           SP P  + CKST YPKLCR+ILS+++ SPSDPY YGKF++K+CLKQA ++S VI  +  R
Sbjct: 80  SPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQR 139

Query: 82  --GGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERIESILSAIVT 141
                     +EV A+ DC EL++ +VE+L+++   L+AAE +   LV+R+ S+L  +VT
Sbjct: 140 VEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLGGVVT 199

Query: 142 NGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSR---RWKKRKQKGDGKG 201
           N QTC+DGLV+++S    A+  PL +  +LYSVSLGLV ++L+R   R+K  K K  G G
Sbjct: 200 NQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKGKIFGGG 259

Query: 202 GKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTT---GILINNTVVVSS 261
            K       REPL TLIK L    +C++   C +  R L +LG T    IL+   V V  
Sbjct: 260 NK-----PVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVGP 319

Query: 262 TGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTV 321
              DNF +I +A++ APN++ P+ GYFVIY R G Y+EYV++   K NIMLIGDGIN+T+
Sbjct: 320 YETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTI 379

Query: 322 ITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCS 381
           I+GNH+ +DGWTTYNSSTF V GD FVA+D+TFRNTAG EKHQAVAVRN+AD STFYRCS
Sbjct: 380 ISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCS 439

Query: 382 FEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGR 441
           FEGYQDTLYVHS+RQFYRECD+YGT+DFIFGNAAA+FQ CN+YARKP+ NQKNA TA GR
Sbjct: 440 FEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGR 499

Query: 442 TDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWL 501
           TDPNQ TGISI NCTI AAPD   D   T  +LGRPWKP+SRTVY+QSYI D++ PVGWL
Sbjct: 500 TDPNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWL 559

Query: 502 EWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYT 561
           EWNGT GLDT+ YGE++N+G GA+TS RV W+G+SL+N+ +AMNFTVYNFT+GDTWLP T
Sbjct: 560 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQT 619

Query: 562 DIPFSRGLL 563
           DIPF  GLL
Sbjct: 620 DIPFYGGLL 621

BLAST of Carg01884 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 505.4 bits (1300), Expect = 6.0e-143
Identity = 277/573 (48.34%), Postives = 369/573 (64.40%), Query Frame = 0

Query: 6   FIFLLFLSL-GLAGASDSPPP---SAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSV 65
           F+  LFLSL  L  AS +  P   S+ +CK+T  PK C+S+    + S  D   YG+FS+
Sbjct: 7   FLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVREYGRFSL 66

Query: 66  KKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQS---IDGVLEA 125
           +K L Q+ K +  I  YL R  ++ L++  VGAL+DCR L+    ++L +      +  +
Sbjct: 67  RKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTS 126

Query: 126 AEGVDEELVERIESILSAIVTNGQTCIDGLVESRSS---LGNALTAPLLSAGKLYSVSLG 185
           ++ +     + I+++LSA +TN QTC+DG+  + SS   + N +  PL++  KL+SVSL 
Sbjct: 127 SKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLA 186

Query: 186 LVKNSLSRRW--KKRKQKGDGKGGKFPAAAQSREPL----NTLIKRLHTMGSCNQSTKCS 245
           L     ++ W  KK+KQ                +P     N  +    T  +       S
Sbjct: 187 L----FTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLS 246

Query: 246 ERKRLLTDLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYY 305
            RK    D     +L+++ V V+  G  NFT+I +A++ APN +    GYFVIYV  G Y
Sbjct: 247 RRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVY 306

Query: 306 DEYVIVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNT 365
           +E V++ K K  +M+IGDGINRTV+TGN NVVDGWTT+NS+TF V    FVA++MTFRNT
Sbjct: 307 EENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNT 366

Query: 366 AGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAV 425
           AG EKHQAVA+R+SADLS FY CSFE YQDTLY HS+RQFYRECD+YGTVDFIFGNAA V
Sbjct: 367 AGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV 426

Query: 426 FQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRP 485
           FQ CNLY R+P+ NQ NA TAQGRTDPNQ+TGISIHNCTIK A D V        YLGRP
Sbjct: 427 FQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRP 486

Query: 486 WKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSL 545
           WK +SRTV+MQSYI +++ PVGW EWNG   L TLYY E+ N G+G++T+ RV W G+ +
Sbjct: 487 WKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHV 546

Query: 546 MNITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL 563
           +N T+A NFTV NF +GD W+  + +P+  GLL
Sbjct: 547 INSTDANNFTVENFLLGDGWMVQSGVPYISGLL 572

BLAST of Carg01884 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 468.8 bits (1205), Expect = 6.2e-132
Identity = 265/582 (45.53%), Postives = 360/582 (61.86%), Query Frame = 0

Query: 7   IFLLFLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLK 66
           I L F    +  +S +   S+ +CK+T  PK C+S+      S  +   YG FS++K L 
Sbjct: 9   ITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSIRKSLS 68

Query: 67  QAIKMSSVIGEYLDRGGDSRLNRQEV-GALEDCRELSDSNVEF-LQSIDGVLEAA----- 126
           Q+ K    +  Y+ R  ++ L++  V  AL+DCR L+   +++ L S + V + +     
Sbjct: 69  QSRKFIRTVDRYIKR--NAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSF 128

Query: 127 EGVDEELVERIESILSAIVTNGQTCIDGLVESRS-----SLGNALTAPLLSAGKLYSVSL 186
           + +     + I+++LSA +TN QTC++GL  + S     ++   +  PL++  KL  VSL
Sbjct: 129 KPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSL 188

Query: 187 GLVKNSLSRRWKKRKQKGDGKGGKFPAAAQSREPLNTLIKRLHTMGS----CNQSTKC-- 246
            L     ++ W  +K+K  G     P +  S    +T   RL   G+      + TK   
Sbjct: 189 AL----FTKGWVPKKKKRAGFAWAQPRSGSS---THTKPFRLFRNGALPLKMTEKTKAVY 248

Query: 247 -SERKRLLTDLGTTG-------ILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYF 306
            S  +R L D  + G       +LI++ V VS  G  NFT+I  A++ APNN+    G+F
Sbjct: 249 ESLSRRKLADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFF 308

Query: 307 VIYVRQGYYDEYVIVPKYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFV 366
           +IYV  G Y+EY+ + K K  +M+IGDGIN+TV+TGN +VVDGWTT+NS+TF V    FV
Sbjct: 309 LIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFV 368

Query: 367 AIDMTFRNTAGAEKHQAVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVD 426
           A+++TFRNTAG EKHQAVA+R+ AD S FY CSFE YQDTLY HS+RQFYRECDVYGTVD
Sbjct: 369 AVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVD 428

Query: 427 FIFGNAAAVFQQCNLYARKPLPNQKNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLN 486
           FIFGNAA VFQ CNLY RKP+PNQ NA TAQGR+DPNQ+TG SI NCTIK A D V    
Sbjct: 429 FIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSNY 488

Query: 487 ETTNYLGRPWKPFSRTVYMQSYIGDLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSS 546
               YLGRPWK +SRTVYMQSYI   + PVGW EWNG   L TLYY E+ N G G+NT++
Sbjct: 489 TVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTN 548

Query: 547 RVNWAGHSLMNITEAMNFTVYNFTMGDTWLPYTDIPFSRGLL 563
           RV W G+ ++N T+A NFTV    +   W+  T +P++ GL+
Sbjct: 549 RVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLI 578

BLAST of Carg01884 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 464.9 bits (1195), Expect = 9.0e-131
Identity = 251/558 (44.98%), Postives = 340/558 (60.93%), Query Frame = 0

Query: 11  FLSLGLAGASDSPPPSAVLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKKCLKQAIK 70
           F+S  +    ++  PS + C+    P  CRS+L +    P D Y YG+ S+++ L +A +
Sbjct: 21  FISAQIPAIGNATSPSNI-CRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARR 80

Query: 71  MSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVLEAAEGVDEELVERI 130
             S+I   LDR G     +  VGALEDC+ L+   +++L S     ++ + +     E +
Sbjct: 81  FISMIDAELDRKGKVAA-KSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDV 140

Query: 131 ESILSAIVTNGQTCIDGLVESRSSLGNALTAPLLSAGKLYSVSLGLVKNSLSRRWKKRKQ 190
            + LSA +TN QTC++GL  + S   N L+  L +  KLY VSL L     S+ W  R+Q
Sbjct: 141 HTFLSAAITNEQTCLEGLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQ 200

Query: 191 KG------DGKGGKFPAAAQSREPLNTLIKRLHTMGSCNQSTKCSERKRLLTDLGTTGIL 250
           +         +  KF      + PL  + +R   +         + RK L +D     + 
Sbjct: 201 RSRPIWQPQARFKKFFGFRNGKLPLK-MTERARAV-----YNTVTRRKLLQSD--ADAVQ 260

Query: 251 INNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVIVPKYKTNIML 310
           +++ V V   G  NFT+I  AI+ APN +   +GYF+IYV  G Y+EYV VPK K  +M+
Sbjct: 261 VSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMM 320

Query: 311 IGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQAVAVRNSA 370
           IGDGIN+TVITGN +VVDGWTT+NS+TF + G  F+ +++T RNTAG  K QAVA+R+  
Sbjct: 321 IGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGG 380

Query: 371 DLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLYARKPLPNQ 430
           DLS FY CSFE YQDTLY HS+RQFYRECDVYGTVDFIFGNAA V Q CNLY R+P   Q
Sbjct: 381 DLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQ 440

Query: 431 KNAFTAQGRTDPNQSTGISIHNCTIKAAPDWVMDLNETTNYLGRPWKPFSRTVYMQSYIG 490
            N  TAQGRTDPNQ+TG +IH CTI+ A D          YLGRPWK +SRTV MQ+YI 
Sbjct: 441 SNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYID 500

Query: 491 DLISPVGWLEWNGTVGLDTLYYGEFENYGAGANTSSRVNWAGHSLMNITEAMNFTVYNFT 550
             + P GW  W+G   L TLYY E+ N G G++T++RV W G+ ++N T+A NFTV NF 
Sbjct: 501 GFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFL 559

Query: 551 MGDTWLPYTDIPFSRGLL 563
           +G+ W+  T +PF  GL+
Sbjct: 561 VGEGWIGQTGVPFVGGLI 559

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032035.10.0e+00100.00putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosp... [more]
KAG6601245.10.0e+0097.88putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosp... [more]
XP_023549932.10.0e+0096.64probable pectinesterase/pectinesterase inhibitor 25 isoform X3 [Cucurbita pepo s... [more]
XP_023549916.10.0e+0095.80probable pectinesterase/pectinesterase inhibitor 25 isoform X1 [Cucurbita pepo s... [more]
XP_023549924.10.0e+0095.62probable pectinesterase/pectinesterase inhibitor 25 isoform X2 [Cucurbita pepo s... [more]
Match NameE-valueIdentityDescription
Q94CB12.0e-19159.74Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Q9FF771.1e-18959.93Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3... [more]
Q8RXK78.5e-14248.34Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
Q9SRX48.8e-13145.53Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
O222561.3e-12944.98Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GYC30.0e+0096.11Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1[more]
A0A6J1INW00.0e+0095.38Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1[more]
A0A1S3BFR94.2e-28586.52Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103489353 PE=3 SV=1[more]
A0A0A0KUN82.7e-28485.99Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G612810 PE=3 SV=1[more]
A0A6J1D8L43.1e-28085.44Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G10720.21.4e-19259.74Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G04970.17.8e-19159.93Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.16.0e-14348.34Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G02810.16.2e-13245.53Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.19.0e-13144.98Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 20..176
e-value: 9.1E-22
score: 88.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..174
e-value: 4.6E-17
score: 62.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 27..180
e-value: 5.3E-23
score: 83.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 29..179
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 243..561
e-value: 1.5E-114
score: 384.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 249..547
e-value: 2.2E-135
score: 450.1
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 6..561
NoneNo IPR availablePANTHERPTHR31707:SF231PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATEDcoord: 6..561
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 29..179
e-value: 8.81525E-28
score: 107.139
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 395..404
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 244..560

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01884-RACarg01884-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity