Carg01775 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01775
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description1-phosphatidylinositol 4-kinase
LocationCarg_Chr04: 9380463 .. 9382400 (-)
RNA-Seq ExpressionCarg01775
SyntenyCarg01775
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCACGGAAGTTGGACAGTCCAGTTCAGACTCAAATGGCAGTTGCAGCACTCAAGAGCCCACTCAGCAGGGCTTACCGCTCGAAAAATGGAATGGAAGAAAAACAGCCAGTTGGAAGGAGACAAGTTTTTGTGCAAACTGATACGGGATGTGTCCTGGGAATGGAATTAGATCGCAGTGACAATGCTCATACGGTGAAAAGGAGGTTGCAGCTTGCCCTCAATATTCCCACAGAGGAGAGCTCCCTAACATTTGGGGATGTGATATTGAAGAATGATCTTAGTGCCATTCGAAATGATTCTCCTCTTCTCCTCACGAGAAATCTGTTGCACAGAAGTTCATCTACTCCATGTCTCTCGCCCACTGGGAAGGATCTCCGTCTTAGAGACCAGAGTGGACCTATTGAGATATTAGGGCATCCAGATCATTTTGCTGGCACGCGACGGTTAGTCAAAGAGATTGTGAAAGGTATAAAGTTGGGTATTGATCCGATTCCTGTTCATAGTGGGCTTGGTGGTGCATACTACTTTAGGAACGGGAAAGGTGAAAGTGTCGCAATTGTGAAGCCAACTGATGAAGAACCTTTTGCACCAAATAATCCAAAAGGTTTTGTTGGAAAAGCTCTTGGACAACCTGGATTTAAGCGCTCTGTGCGTGTTGGGGAGACTGGATTCAGAGAAGTTGCAGCTTATCTTCTTGACTATGAACATTTTGCAAACGTGCCTCCGACAGCCTTAGTGAAGATCACTCACTCAATCTTCAATGTAAATGATTGGGTGAATGGTAACAAGCCTCAGGCTCACAAGAAAAAGCCAGTTAGTAAAATTGCTTCCTTCCAACAATTCATTCCACATGATTTTGATGCCAGTGATCATGGGACTTCTAGTTTCCCAGTCGCGGCTGTTCATCGTATAGGTATTTTAGATATTAGGATTTTTAATACGGATAGGCATGCTGGGAATCTCTTAGTTCGGAAACTTGACGGAATAGGAAGTTTTGGACAAGTGGAGCTTATTCCTATAGATCACGGTCTTTGTTTGCCGGAAACTTTAGAGGATCCGTACTTTGAGTGGATTCATTGGCCTCAAGCATCAATCCCATTTTCAGATGATGAGCTCAAGTATATTGAAAATCTTGATCCGATAAAAGACTGTGAGATGCTTAGGATGGAGCTACCCATGATCCGTGAAGCTTGTCTTCGGGTTTTGGTACTGTGTACAATTTTTCTGAAGGAAGCTGCTGCTTCTGGTCTCTGTCTTGCTGAGATTGGTGAGATGATGAGCAGAGAATTTCGTAGTGGAGAAGAGGAACCCAGTGAACTTGAGCTAGTATGTCTTGAGGCTAGGCAGATGATAGCAGAGAGGGAGTTCTCTTCTCCAATATATGAGTGGGGCAATGAGGATTTCCAGTTTGACTTAGACTGTGAGGATGGAGAATTAGACTATAACTCAAGTATGGCAGCGGATCAGTGTTTGGGCTGGTCACATTACCAGCTTGGTGGACTTGTTGGAGGCCTGCATGGACGCAATCCACTCTCGAAATTGGAGGAAAGCATTGAGGAAGGGGGAGAAGATAGTGGAGGGGAGGAGCTAGGAGAAACAGGCATCTTTCCACCTTCTGAACCCATTTCTGTGTCAAAGCTTTCTATGTCTCTCAAGAATACCATACTGGGCAATAAGAACCAGAAAAATCAAAAGTACCAGGTCACAAAACCTGAGTTTGGATATATTACCAACACATCTGCAGGGCACAGGAGTGCAAACGAGCAGTTTCCTCCAAGTGTCAGTTTTGTAAAGCTTGGAGATATGAACGAGGAAGACTGGTCGCTGTTCCTGGATAAATTTCCAGAGCTGCTTCGCCCAGCGTTTGCCAAACGCAAATCTGCAGCTCTCGGGCCGAGGCAGAGGCAGAGACTTGGTACTTCTTGCCAGTTTTGA

mRNA sequence

ATGTCACGGAAGTTGGACAGTCCAGTTCAGACTCAAATGGCAGTTGCAGCACTCAAGAGCCCACTCAGCAGGGCTTACCGCTCGAAAAATGGAATGGAAGAAAAACAGCCAGTTGGAAGGAGACAAGTTTTTGTGCAAACTGATACGGGATGTGTCCTGGGAATGGAATTAGATCGCAGTGACAATGCTCATACGGTGAAAAGGAGGTTGCAGCTTGCCCTCAATATTCCCACAGAGGAGAGCTCCCTAACATTTGGGGATGTGATATTGAAGAATGATCTTAGTGCCATTCGAAATGATTCTCCTCTTCTCCTCACGAGAAATCTGTTGCACAGAAGTTCATCTACTCCATGTCTCTCGCCCACTGGGAAGGATCTCCGTCTTAGAGACCAGAGTGGACCTATTGAGATATTAGGGCATCCAGATCATTTTGCTGGCACGCGACGGTTAGTCAAAGAGATTGTGAAAGGTATAAAGTTGGGTATTGATCCGATTCCTGTTCATAGTGGGCTTGGTGGTGCATACTACTTTAGGAACGGGAAAGGTGAAAGTGTCGCAATTGTGAAGCCAACTGATGAAGAACCTTTTGCACCAAATAATCCAAAAGGTTTTGTTGGAAAAGCTCTTGGACAACCTGGATTTAAGCGCTCTGTGCGTGTTGGGGAGACTGGATTCAGAGAAGTTGCAGCTTATCTTCTTGACTATGAACATTTTGCAAACGTGCCTCCGACAGCCTTAGTGAAGATCACTCACTCAATCTTCAATGTAAATGATTGGGTGAATGGTAACAAGCCTCAGGCTCACAAGAAAAAGCCAGTTAGTAAAATTGCTTCCTTCCAACAATTCATTCCACATGATTTTGATGCCAGTGATCATGGGACTTCTAGTTTCCCAGTCGCGGCTGTTCATCGTATAGGTATTTTAGATATTAGGATTTTTAATACGGATAGGCATGCTGGGAATCTCTTAGTTCGGAAACTTGACGGAATAGGAAGTTTTGGACAAGTGGAGCTTATTCCTATAGATCACGGTCTTTGTTTGCCGGAAACTTTAGAGGATCCGTACTTTGAGTGGATTCATTGGCCTCAAGCATCAATCCCATTTTCAGATGATGAGCTCAAGTATATTGAAAATCTTGATCCGATAAAAGACTGTGAGATGCTTAGGATGGAGCTACCCATGATCCGTGAAGCTTGTCTTCGGGTTTTGGTACTGTGTACAATTTTTCTGAAGGAAGCTGCTGCTTCTGGTCTCTGTCTTGCTGAGATTGGTGAGATGATGAGCAGAGAATTTCGTAGTGGAGAAGAGGAACCCAGTGAACTTGAGCTAGTATGTCTTGAGGCTAGGCAGATGATAGCAGAGAGGGAGTTCTCTTCTCCAATATATGAGTGGGGCAATGAGGATTTCCAGTTTGACTTAGACTGTGAGGATGGAGAATTAGACTATAACTCAAGTATGGCAGCGGATCAGTGTTTGGGCTGGTCACATTACCAGCTTGGTGGACTTGTTGGAGGCCTGCATGGACGCAATCCACTCTCGAAATTGGAGGAAAGCATTGAGGAAGGGGGAGAAGATAGTGGAGGGGAGGAGCTAGGAGAAACAGGCATCTTTCCACCTTCTGAACCCATTTCTGTGTCAAAGCTTTCTATGTCTCTCAAGAATACCATACTGGGCAATAAGAACCAGAAAAATCAAAAGTACCAGGTCACAAAACCTGAGTTTGGATATATTACCAACACATCTGCAGGGCACAGGAGTGCAAACGAGCAGTTTCCTCCAAGTGTCAGTTTTGTAAAGCTTGGAGATATGAACGAGGAAGACTGGTCGCTGTTCCTGGATAAATTTCCAGAGCTGCTTCGCCCAGCGTTTGCCAAACGCAAATCTGCAGCTCTCGGGCCGAGGCAGAGGCAGAGACTTGGTACTTCTTGCCAGTTTTGA

Coding sequence (CDS)

ATGTCACGGAAGTTGGACAGTCCAGTTCAGACTCAAATGGCAGTTGCAGCACTCAAGAGCCCACTCAGCAGGGCTTACCGCTCGAAAAATGGAATGGAAGAAAAACAGCCAGTTGGAAGGAGACAAGTTTTTGTGCAAACTGATACGGGATGTGTCCTGGGAATGGAATTAGATCGCAGTGACAATGCTCATACGGTGAAAAGGAGGTTGCAGCTTGCCCTCAATATTCCCACAGAGGAGAGCTCCCTAACATTTGGGGATGTGATATTGAAGAATGATCTTAGTGCCATTCGAAATGATTCTCCTCTTCTCCTCACGAGAAATCTGTTGCACAGAAGTTCATCTACTCCATGTCTCTCGCCCACTGGGAAGGATCTCCGTCTTAGAGACCAGAGTGGACCTATTGAGATATTAGGGCATCCAGATCATTTTGCTGGCACGCGACGGTTAGTCAAAGAGATTGTGAAAGGTATAAAGTTGGGTATTGATCCGATTCCTGTTCATAGTGGGCTTGGTGGTGCATACTACTTTAGGAACGGGAAAGGTGAAAGTGTCGCAATTGTGAAGCCAACTGATGAAGAACCTTTTGCACCAAATAATCCAAAAGGTTTTGTTGGAAAAGCTCTTGGACAACCTGGATTTAAGCGCTCTGTGCGTGTTGGGGAGACTGGATTCAGAGAAGTTGCAGCTTATCTTCTTGACTATGAACATTTTGCAAACGTGCCTCCGACAGCCTTAGTGAAGATCACTCACTCAATCTTCAATGTAAATGATTGGGTGAATGGTAACAAGCCTCAGGCTCACAAGAAAAAGCCAGTTAGTAAAATTGCTTCCTTCCAACAATTCATTCCACATGATTTTGATGCCAGTGATCATGGGACTTCTAGTTTCCCAGTCGCGGCTGTTCATCGTATAGGTATTTTAGATATTAGGATTTTTAATACGGATAGGCATGCTGGGAATCTCTTAGTTCGGAAACTTGACGGAATAGGAAGTTTTGGACAAGTGGAGCTTATTCCTATAGATCACGGTCTTTGTTTGCCGGAAACTTTAGAGGATCCGTACTTTGAGTGGATTCATTGGCCTCAAGCATCAATCCCATTTTCAGATGATGAGCTCAAGTATATTGAAAATCTTGATCCGATAAAAGACTGTGAGATGCTTAGGATGGAGCTACCCATGATCCGTGAAGCTTGTCTTCGGGTTTTGGTACTGTGTACAATTTTTCTGAAGGAAGCTGCTGCTTCTGGTCTCTGTCTTGCTGAGATTGGTGAGATGATGAGCAGAGAATTTCGTAGTGGAGAAGAGGAACCCAGTGAACTTGAGCTAGTATGTCTTGAGGCTAGGCAGATGATAGCAGAGAGGGAGTTCTCTTCTCCAATATATGAGTGGGGCAATGAGGATTTCCAGTTTGACTTAGACTGTGAGGATGGAGAATTAGACTATAACTCAAGTATGGCAGCGGATCAGTGTTTGGGCTGGTCACATTACCAGCTTGGTGGACTTGTTGGAGGCCTGCATGGACGCAATCCACTCTCGAAATTGGAGGAAAGCATTGAGGAAGGGGGAGAAGATAGTGGAGGGGAGGAGCTAGGAGAAACAGGCATCTTTCCACCTTCTGAACCCATTTCTGTGTCAAAGCTTTCTATGTCTCTCAAGAATACCATACTGGGCAATAAGAACCAGAAAAATCAAAAGTACCAGGTCACAAAACCTGAGTTTGGATATATTACCAACACATCTGCAGGGCACAGGAGTGCAAACGAGCAGTTTCCTCCAAGTGTCAGTTTTGTAAAGCTTGGAGATATGAACGAGGAAGACTGGTCGCTGTTCCTGGATAAATTTCCAGAGCTGCTTCGCCCAGCGTTTGCCAAACGCAAATCTGCAGCTCTCGGGCCGAGGCAGAGGCAGAGACTTGGTACTTCTTGCCAGTTTTGA

Protein sequence

MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRSDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLSPTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGELDYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPSEPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKLGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Homology
BLAST of Carg01775 vs. NCBI nr
Match: KAG6601359.1 (Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032143.1 Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 645/645 (100.00%), Postives = 645/645 (100.00%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS
Sbjct: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540

Query: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600
           EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Sbjct: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 645

BLAST of Carg01775 vs. NCBI nr
Match: XP_022956825.1 (phosphatidylinositol 4-kinase gamma 5-like [Cucurbita moschata] >XP_022956826.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita moschata] >XP_022956827.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita moschata])

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 640/645 (99.22%), Postives = 643/645 (99.69%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHPDHFAGTR+LVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRQLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DYNSSM ADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS
Sbjct: 481 DYNSSMTADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540

Query: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600
           EPISVSKLSMSLKNT+LGNKNQKNQ +QVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 EPISVSKLSMSLKNTVLGNKNQKNQNHQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Sbjct: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 645

BLAST of Carg01775 vs. NCBI nr
Match: XP_023533870.1 (phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo] >XP_023533879.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo] >XP_023533888.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1295.4 bits (3351), Expect = 0.0e+00
Identity = 640/645 (99.22%), Postives = 642/645 (99.53%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVH GLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHGGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKK VSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKAVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWG+EDFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGSEDFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DYNSSMAADQCLGWSHYQLGGL GGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS
Sbjct: 481 DYNSSMAADQCLGWSHYQLGGL-GGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540

Query: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600
           EPISVSKLSMSLKNT+LGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 EPISVSKLSMSLKNTVLGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Sbjct: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 644

BLAST of Carg01775 vs. NCBI nr
Match: XP_022976415.1 (phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima] >XP_022976424.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima] >XP_022976432.1 phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima])

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 639/645 (99.07%), Postives = 640/645 (99.22%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDP PVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPTPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVN+WVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNNWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKD EMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDSEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DYNSSMAADQCLGWSHYQLGGL GGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS
Sbjct: 481 DYNSSMAADQCLGWSHYQLGGL-GGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540

Query: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600
           EPISVSKLSMSLKNTILGNKNQK  KYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 EPISVSKLSMSLKNTILGNKNQKTPKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Sbjct: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 644

BLAST of Carg01775 vs. NCBI nr
Match: XP_008446479.1 (PREDICTED: phosphatidylinositol 4-kinase gamma 5 [Cucumis melo] >XP_008446480.1 PREDICTED: phosphatidylinositol 4-kinase gamma 5 [Cucumis melo] >KAA0034489.1 phosphatidylinositol 4-kinase gamma 5 [Cucumis melo var. makuwa] >TYK09043.1 phosphatidylinositol 4-kinase gamma 5 [Cucumis melo var. makuwa])

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 612/646 (94.74%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSR Y SKNG +EKQPVGRRQVFVQTDTGCVLGMELDR+
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKQPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGH D F+GT+RLVKEIVK IKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKK VSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQ+IAEREFSSP  +W NE+FQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSDWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DY+S MAADQCLGWSHYQ GGL G LHGRNPLSKLEESIEE GEDSGGEE GET  F PS
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGL-GSLHGRNPLSKLEESIEEEGEDSGGEEQGETAAFLPS 540

Query: 541 EPIS-VSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVK 600
           EPIS VSKLSMSLKNTILG+KNQKNQKYQVTKPE+GYITNTSAGHRSANEQFPPSVSFVK
Sbjct: 541 EPISVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVK 600

Query: 601 LGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           LGDMNEE+WSLFLDKFPELLRPAFAKRKSA LG RQRQRLGTSCQF
Sbjct: 601 LGDMNEEEWSLFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Carg01775 vs. ExPASy Swiss-Prot
Match: Q9SI52 (Phosphatidylinositol 4-kinase gamma 7 OS=Arabidopsis thaliana OX=3702 GN=PI4KG7 PE=1 SV=2)

HSP 1 Score: 870.2 bits (2247), Expect = 1.5e-251
Identity = 454/668 (67.96%), Postives = 528/668 (79.04%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEK------QPVGRRQVFVQTDTGCVLG 60
           MSR LDSPVQTQMAVA  K+PL+ A + +     K      Q  GRR VFVQT+TGCVLG
Sbjct: 1   MSRNLDSPVQTQMAVAVFKTPLTGASKMEGKQHHKHQHLQRQSSGRR-VFVQTETGCVLG 60

Query: 61  MELDRSDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSS 120
           MELDRSDN HTVKRRLQ+ALN PTEESSLT+GD++L NDLSA+RNDSPLLL RN +HRSS
Sbjct: 61  MELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSAVRNDSPLLLKRNFMHRSS 120

Query: 121 STPCLSPTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGA 180
           STPCLSPTG+DL+ +D+SGPIEILGH D F+  + +VK+IVK +K+G++P+PVHSGLGGA
Sbjct: 121 STPCLSPTGRDLQQKDRSGPIEILGHSDCFSIVKHMVKDIVKAMKMGVEPLPVHSGLGGA 180

Query: 181 YYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLD 240
           YYFRN +GESVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLD
Sbjct: 181 YYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVRVGETGFREVAAYLLD 240

Query: 241 YEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGT 300
           Y  FANVPPTALVKITHS+FNVND V GNKP+  +KK VSKIASFQ+F+ HDFDASDHGT
Sbjct: 241 YGRFANVPPTALVKITHSVFNVNDGVKGNKPR--EKKLVSKIASFQKFVAHDFDASDHGT 300

Query: 301 SSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDP 360
           SSFPV +VHRIGILDIRIFNTDRH GNLLV+KLDG+G FGQVELIPIDHGLCLPETLEDP
Sbjct: 301 SSFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDP 360

Query: 361 YFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAA 420
           YFEWIHWPQAS+PFSD+E+ YI++LDP+KDC+MLR ELPMIREACLRVLVLCTIFLKEA+
Sbjct: 361 YFEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEAS 420

Query: 421 ASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYE-WGNEDFQFDL 480
           A GLCLAEIGEMM+REFR GEEEPSELE+VC+EA++ + ER+  SP  +  G  +FQFDL
Sbjct: 421 AYGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDL 480

Query: 481 DCEDGELDYNSSM-AADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEE-- 540
           DC+D E  Y+S +   D     + +         +GR+ L KLEESI+E  ED   EE  
Sbjct: 481 DCDDLESVYSSKIQLTDDYFTKNPFS--------NGRSSLGKLEESIKEEEEDEEEEEDK 540

Query: 541 -------------LGETGIFPPSEPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYI 600
                           +  F    P S+SKLS S+KNT L +  +KN K  +T+   G  
Sbjct: 541 TENTVPMIIMKDSFFSSAAFHDKAP-SLSKLSTSMKNTHLSDTTRKNPK-PLTR---GKS 600

Query: 601 TNTSAGHRSANEQFPPSVSFVKLGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQ 646
            NTS+GH+SANEQ P S SFVK+ DM E++W LFL++F ELL PAFAKRK+A L   +RQ
Sbjct: 601 ENTSSGHKSANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQ 650

BLAST of Carg01775 vs. ExPASy Swiss-Prot
Match: Q9C671 (Phosphatidylinositol 4-kinase gamma 5 OS=Arabidopsis thaliana OX=3702 GN=PI4KG5 PE=1 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 5.3e-249
Identity = 441/647 (68.16%), Postives = 517/647 (79.91%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSP++TQMAVA +KSPL+  +R  N +  K PVGRR+VFVQT+TGCVLG+ELDRS
Sbjct: 1   MSRKLDSPIKTQMAVALVKSPLNGEFREFNKVGMKTPVGRRRVFVQTETGCVLGLELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKR+LQ+ALN P EESSLTFGD++LKNDL+A+R+DSPLLLTRN  HRSSSTPCLS
Sbjct: 61  DNAHTVKRKLQVALNFPIEESSLTFGDLVLKNDLTAVRSDSPLLLTRNNFHRSSSTPCLS 120

Query: 121 PTGKDL-RLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRN 180
           P   DL + RD+S PIEILG+   F+  R++ K+I K +K GIDP+ V+SGLGGAYYF+N
Sbjct: 121 PMRADLQQRRDESSPIEILGNSVSFSFVRQMAKDITKAVKKGIDPVAVNSGLGGAYYFKN 180

Query: 181 GKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFA 240
            +GESVAIVKPTDEEP+APNNPKGFVGKALGQPG KRSVRVGETG+REVAAYLLD EHFA
Sbjct: 181 SRGESVAIVKPTDEEPYAPNNPKGFVGKALGQPGLKRSVRVGETGYREVAAYLLDKEHFA 240

Query: 241 NVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPV 300
           NVPPTALVKITHSIFNVND V  +KP   +K  VSKIAS QQFIPHD+DAS+HGTS+FPV
Sbjct: 241 NVPPTALVKITHSIFNVNDGVKASKPM--EKMLVSKIASLQQFIPHDYDASEHGTSNFPV 300

Query: 301 AAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWI 360
           +AVHRIGILDIRI NTDRH+GNLLV+KLDG G FGQVEL+PIDHGLCLPETLEDPYFEWI
Sbjct: 301 SAVHRIGILDIRILNTDRHSGNLLVKKLDGDGMFGQVELVPIDHGLCLPETLEDPYFEWI 360

Query: 361 HWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLC 420
           HWPQASIPFS+DELKYI NLDP+ DCEMLR ELPM+REA LRVLVLCTIFLKEAAA+GLC
Sbjct: 361 HWPQASIPFSEDELKYIANLDPLGDCEMLRRELPMVREASLRVLVLCTIFLKEAAANGLC 420

Query: 421 LAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNE-DFQFDLDCEDG 480
           LAEIGEMM+RE R G+EEPSE+E+VCLEA  +I E++  SP  + GN+ +FQFD+DCE+ 
Sbjct: 421 LAEIGEMMTREVRPGDEEPSEIEVVCLEAMSLIGEKDAESPRSDLGNDIEFQFDIDCEE- 480

Query: 481 ELDYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFP 540
                       C       LG   G   G+  LSK+EE+ E+G E+   +   E     
Sbjct: 481 ---------VTDCTKKLALPLGLTFGNARGQ--LSKVEETTEDGEEEEEEDREEEENDRA 540

Query: 541 PSEPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFV 600
             E +   KLSMSLK+T+LG K+QK QK+   + E  Y    S+ HRSA+EQ P S SFV
Sbjct: 541 DLEKMPTIKLSMSLKSTLLGEKSQKYQKHPGARVESAY---ASSAHRSADEQIPSSTSFV 600

Query: 601 KLGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           KL DM+EE+W++FL+K+ ELL PA  KRKS  LG +QRQRLGTSCQF
Sbjct: 601 KLSDMSEEEWTIFLEKYQELLYPAIEKRKSITLGQKQRQRLGTSCQF 630

BLAST of Carg01775 vs. ExPASy Swiss-Prot
Match: Q8W4R8 (Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 1.0e-231
Identity = 412/645 (63.88%), Postives = 492/645 (76.28%), Query Frame = 0

Query: 13  MAVAALKSPLSRAYRSKNGME---------EKQPVGRRQVFVQTDTGCVLGMELDRSDNA 72
           MA+A  K+PL   +     ME         ++Q  GRR+VFVQTDTGCVLG+ELDR+DN 
Sbjct: 1   MAMAVFKAPLKGEFHGARKMEGKQYKHHLLQQQSTGRRRVFVQTDTGCVLGVELDRNDNV 60

Query: 73  HTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLSPTG 132
           HTVK+RLQ+A N PTEESSLTFGD++LKNDLSA+RNDSPLLL RNL+HRSSSTPCLSPTG
Sbjct: 61  HTVKKRLQIAFNFPTEESSLTFGDMVLKNDLSAVRNDSPLLLKRNLMHRSSSTPCLSPTG 120

Query: 133 KDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNGKGE 192
            DL+ +D+SGPIEIL H   F   ++   +IVK +K+G++PIPV+ GLGGAYYFR+ KG+
Sbjct: 121 NDLQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRDEKGQ 180

Query: 193 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFANVPP 252
           SVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLDY+HFANVPP
Sbjct: 181 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDYDHFANVPP 240

Query: 253 TALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVAAVH 312
           TALVKITHS+FNVND ++GNK +  KK   SKIASFQ+F+PHDFDASDHGTSSFPVA+VH
Sbjct: 241 TALVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHDFDASDHGTSSFPVASVH 300

Query: 313 RIGILDIRIFNTDRHAGNLLVRKLD--GIGSFGQVELIPIDHGLCLPETLEDPYFEWIHW 372
           RIGILDIRI NTDRH GNLLV+KLD  G+G FGQVELIPIDHGLCLPETLEDPYFEWIHW
Sbjct: 301 RIGILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHW 360

Query: 373 PQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCLA 432
           PQASIPFS++EL YI++LDP+KDCEMLR ELPMIREACLRVLVLCT+FLKEAA  GLCLA
Sbjct: 361 PQASIPFSEEELDYIQSLDPVKDCEMLRRELPMIREACLRVLVLCTVFLKEAAVFGLCLA 420

Query: 433 EIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEW-GNEDFQFDLDCEDGEL 492
           EIGEMM+REFR+GEEEPSELE++C+EA+++  E++  SP  +  G  +FQFD+D  + + 
Sbjct: 421 EIGEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPKSDGEGETEFQFDIDYNELDS 480

Query: 493 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 552
            Y S    D+    + +         +GR+ L +L+ESI E  ED   E           
Sbjct: 481 VYGSETETDEFFAKNPFS--------NGRSSLGELKESIAEEEEDDEEE---------AK 540

Query: 553 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 612
             +S+SKLS S+KN +    N     Y +  P+          H+SAN Q P SV+FVKL
Sbjct: 541 LTLSLSKLSTSMKNNL---SNTMGSGY-LKPPKDNQTDKALVSHKSANVQLPLSVNFVKL 600

Query: 613 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
            DM E +W +FL++F ELL  AFA+RK+  L  R  QRLGTSC+F
Sbjct: 601 ADMKEVEWVVFLERFQELLYSAFAERKTMTL--RNTQRLGTSCKF 622

BLAST of Carg01775 vs. ExPASy Swiss-Prot
Match: Q0WMZ6 (Phosphatidylinositol 4-kinase gamma 8 OS=Arabidopsis thaliana OX=3702 GN=PI4KG8 PE=2 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 9.2e-100
Identity = 233/559 (41.68%), Postives = 307/559 (54.92%), Query Frame = 0

Query: 106 TRNLLHRSSSTPCLSPTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPI 165
           + +L +RS STPC S +G +L     +  IEILG        R LV E+   I  G  P+
Sbjct: 48  SHSLNNRSVSTPCFSISGSNLD-GSATPHIEILG-GQRVPTVRSLVAEVTIAIVSGAQPL 107

Query: 166 PVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGF 225
            + SGLGGAY  +  KG ++A+ KP DEEP A NNPKG  G  LGQPG KRS+RVGE+G 
Sbjct: 108 LLPSGLGGAYLLQTEKGNNIAVAKPVDEEPLAFNNPKGSGGLTLGQPGMKRSIRVGESGI 167

Query: 226 REVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPH 285
           RE+AAYLLD++ F++VPPTALV+I+H  F+      G+   A+      K+AS Q+F+ H
Sbjct: 168 RELAAYLLDHQGFSSVPPTALVRISHVPFH----DRGSDHAAY------KVASLQRFVGH 227

Query: 286 DFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDG------IGSFGQVELI 345
           DFDA + G  SF V +VHRIGILD+R+ N DRHAGN+LV+K+            G  EL+
Sbjct: 228 DFDAGELGPGSFTVVSVHRIGILDVRVLNLDRHAGNMLVKKIHDQDETTCSNGVGAAELV 287

Query: 346 PIDHGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREAC 405
           PIDHGLCLPE L+DPYFEW++WPQAS+PF+D EL+YI NLDP KD E+LR EL  I+E+ 
Sbjct: 288 PIDHGLCLPECLDDPYFEWLNWPQASVPFTDIELQYISNLDPFKDAELLRTELDSIQESS 347

Query: 406 LRVLVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSS 465
           LRVL++CTIFLKEAAA+GL LAEIGE M+R+   GEE  S LE++C +A+          
Sbjct: 348 LRVLIVCTIFLKEAAAAGLSLAEIGEKMTRDICRGEESSSVLEILCNKAKASAVSGSDDD 407

Query: 466 PIY--EWGNED-------FQFDLDCEDGELDYNSSMAADQCLGWSHYQLGGLVGGLHGRN 525
             Y  EW   +       FQFD + E  EL     +     +      L  L+     R 
Sbjct: 408 DDYSSEWNEVEAELECGIFQFDDEVECKELPDMLQVPIFTRVPSIAANLSALM-----RC 467

Query: 526 P----LSKLEESIEEGGEDSGGEELGETGIFPPSEPISVSKLSMSLKNTILGNKNQKNQK 585
           P    +S  + +IEE       E    + +   S PI V             N ++K   
Sbjct: 468 PPNQWISTYDTNIEE-------ERRDRSIVRSKSHPICV-------------NYDEKEGV 527

Query: 586 YQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKLGDMNEEDWSLFLDKFPELLRPAFAKR 645
           Y                                 GDM+ ++W +FL  F  LL  A    
Sbjct: 528 Y--------------------------------FGDMSGDEWEMFLHSFQMLLPEAL--E 533

BLAST of Carg01775 vs. ExPASy Swiss-Prot
Match: O22199 (Phosphatidylinositol 4-kinase gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KG1 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.3e-98
Identity = 186/373 (49.87%), Postives = 241/373 (64.61%), Query Frame = 0

Query: 110 LHRSSSTPCLS---PTGKDLRLRDQSGP-IEILGHPDHFAGTRRLVKEIVKGIKLGIDPI 169
           +HRS S+PC S       +  L+  + P IEILG        R LV E+   +  G  P+
Sbjct: 67  IHRSVSSPCFSIAASANMEEDLKATTAPRIEILG-GQRVPTVRALVAEVTMAMVSGAQPL 126

Query: 170 PVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGF 229
            + SG+GGAY  + GKG ++A+ KP DEEP A NNPK      LGQPG K S+ VGETG 
Sbjct: 127 LLPSGMGGAYLLQTGKGHNIAVAKPVDEEPLAFNNPKKSGNLMLGQPGMKHSIPVGETGI 186

Query: 230 REVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPH 289
           RE+AAYLLDY+ F+ VPPTALV I+H  F+V+D  + +           K+AS Q+F+ H
Sbjct: 187 RELAAYLLDYQGFSGVPPTALVSISHVPFHVSDAFSFSSMP-------YKVASLQRFVGH 246

Query: 290 DFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLD---GIGSFGQVELIPID 349
           DFDA + G  SF   +VHRIGILD+R+ N DRHAGN+LV++ D        G  EL+PID
Sbjct: 247 DFDAGELGPGSFTATSVHRIGILDVRLLNLDRHAGNMLVKRCDKKEAYNRLGTAELVPID 306

Query: 350 HGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRV 409
           HGLCLPE L+DPYFEW++WPQA +PFSD EL YI NLDP KD E+LR EL  + E+ +RV
Sbjct: 307 HGLCLPECLDDPYFEWLNWPQALVPFSDTELDYISNLDPFKDAELLRTELHSLPESAIRV 366

Query: 410 LVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIY 469
           LV+CT+FLK+AAA+GLCLAEIGE M+R+F  GEE  S LE +C +A+  +  +      Y
Sbjct: 367 LVVCTVFLKQAAAAGLCLAEIGEKMTRDFSKGEESFSLLETLCTKAKASVFGKTSEDSDY 426

Query: 470 EWGNEDFQFDLDC 476
                +   +L C
Sbjct: 427 SHEGNEVNTELQC 431

BLAST of Carg01775 vs. ExPASy TrEMBL
Match: A0A6J1GYU5 (1-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111458409 PE=3 SV=1)

HSP 1 Score: 1301.2 bits (3366), Expect = 0.0e+00
Identity = 640/645 (99.22%), Postives = 643/645 (99.69%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHPDHFAGTR+LVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRQLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DYNSSM ADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS
Sbjct: 481 DYNSSMTADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540

Query: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600
           EPISVSKLSMSLKNT+LGNKNQKNQ +QVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 EPISVSKLSMSLKNTVLGNKNQKNQNHQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Sbjct: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 645

BLAST of Carg01775 vs. ExPASy TrEMBL
Match: A0A6J1IJE7 (1-phosphatidylinositol 4-kinase OS=Cucurbita maxima OX=3661 GN=LOC111476826 PE=3 SV=1)

HSP 1 Score: 1290.0 bits (3337), Expect = 0.0e+00
Identity = 639/645 (99.07%), Postives = 640/645 (99.22%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDP PVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPTPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVN+WVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNNWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKD EMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDSEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DYNSSMAADQCLGWSHYQLGGL GGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS
Sbjct: 481 DYNSSMAADQCLGWSHYQLGGL-GGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540

Query: 541 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600
           EPISVSKLSMSLKNTILGNKNQK  KYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL
Sbjct: 541 EPISVSKLSMSLKNTILGNKNQKTPKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 600

Query: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF
Sbjct: 601 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 644

BLAST of Carg01775 vs. ExPASy TrEMBL
Match: A0A1S3BFU2 (1-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103489203 PE=3 SV=1)

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 612/646 (94.74%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSR Y SKNG +EKQPVGRRQVFVQTDTGCVLGMELDR+
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKQPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGH D F+GT+RLVKEIVK IKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKK VSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQ+IAEREFSSP  +W NE+FQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSDWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DY+S MAADQCLGWSHYQ GGL G LHGRNPLSKLEESIEE GEDSGGEE GET  F PS
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGL-GSLHGRNPLSKLEESIEEEGEDSGGEEQGETAAFLPS 540

Query: 541 EPIS-VSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVK 600
           EPIS VSKLSMSLKNTILG+KNQKNQKYQVTKPE+GYITNTSAGHRSANEQFPPSVSFVK
Sbjct: 541 EPISVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVK 600

Query: 601 LGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           LGDMNEE+WSLFLDKFPELLRPAFAKRKSA LG RQRQRLGTSCQF
Sbjct: 601 LGDMNEEEWSLFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Carg01775 vs. ExPASy TrEMBL
Match: A0A5D3CB14 (1-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00300 PE=3 SV=1)

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 612/646 (94.74%), Postives = 623/646 (96.44%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSR Y SKNG +EKQPVGRRQVFVQTDTGCVLGMELDR+
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKQPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGH D F+GT+RLVKEIVK IKLGIDPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSDRFSGTKRLVKEIVKAIKLGIDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNKPQAHKKK VSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQ+IAEREFSSP  +W NE+FQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSDWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DY+S MAADQCLGWSHYQ GGL G LHGRNPLSKLEESIEE GEDSGGEE GET  F PS
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGL-GSLHGRNPLSKLEESIEEEGEDSGGEEQGETAAFLPS 540

Query: 541 EPIS-VSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVK 600
           EPIS VSKLSMSLKNTILG+KNQKNQKYQVTKPE+GYITNTSAGHRSANEQFPPSVSFVK
Sbjct: 541 EPISVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVK 600

Query: 601 LGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           LGDMNEE+WSLFLDKFPELLRPAFAKRKSA LG RQRQRLGTSCQF
Sbjct: 601 LGDMNEEEWSLFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Carg01775 vs. ExPASy TrEMBL
Match: A0A0A0KWA5 (1-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G606910 PE=3 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 607/646 (93.96%), Postives = 622/646 (96.28%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSPVQTQMAVAALKSPLSR Y SKNG +EK PVGRRQVFVQTDTGCVLGMELDR+
Sbjct: 1   MSRKLDSPVQTQMAVAALKSPLSRDYHSKNGRDEKLPVGRRQVFVQTDTGCVLGMELDRN 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS
Sbjct: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120

Query: 121 PTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNG 180
           PTGKDLRLRDQSGPIEILGH + F+GT+RLVKEIVK IKLG+DPIPVHSGLGGAYYFRNG
Sbjct: 121 PTGKDLRLRDQSGPIEILGHSERFSGTKRLVKEIVKAIKLGVDPIPVHSGLGGAYYFRNG 180

Query: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240
           KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN
Sbjct: 181 KGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFAN 240

Query: 241 VPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVA 300
           VPPTALVKITHSIFNVNDWVNGNK QAHKKK VSKIASFQQFIPHDFDASDHGTSSFPVA
Sbjct: 241 VPPTALVKITHSIFNVNDWVNGNKSQAHKKKSVSKIASFQQFIPHDFDASDHGTSSFPVA 300

Query: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWIH 360
           AVHRIGILDIRIFNTDRHAGNLLVRKLDGIG+FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 AVHRIGILDIRIFNTDRHAGNLLVRKLDGIGTFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420
           WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL
Sbjct: 361 WPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCL 420

Query: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNEDFQFDLDCEDGEL 480
           AEIGEMMSREFRSGEEEPSELELVCLEARQ+IAEREFSSP  EW NE+FQFDLDCEDGEL
Sbjct: 421 AEIGEMMSREFRSGEEEPSELELVCLEARQIIAEREFSSPTSEWSNEEFQFDLDCEDGEL 480

Query: 481 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 540
           DY+S MAADQCLGWSHYQ GGL G LHGRNPLSKLEESIEE GEDSGGEE GET  F PS
Sbjct: 481 DYSSGMAADQCLGWSHYQFGGL-GSLHGRNPLSKLEESIEEEGEDSGGEEQGETASFLPS 540

Query: 541 EPIS-VSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVK 600
           EP+S VSKLSMSLKNTILG+KNQKNQKYQVTKPE+GYITNTSAGHRSANEQFPPSVSFVK
Sbjct: 541 EPVSVVSKLSMSLKNTILGDKNQKNQKYQVTKPEYGYITNTSAGHRSANEQFPPSVSFVK 600

Query: 601 LGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           LGDMNEE+WS+FLDKFPELLRPAFAKRKSA LG RQRQRLGTSCQF
Sbjct: 601 LGDMNEEEWSIFLDKFPELLRPAFAKRKSAVLGQRQRQRLGTSCQF 645

BLAST of Carg01775 vs. TAIR 10
Match: AT2G03890.1 (phosphoinositide 4-kinase gamma 7 )

HSP 1 Score: 870.2 bits (2247), Expect = 1.1e-252
Identity = 454/668 (67.96%), Postives = 528/668 (79.04%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEK------QPVGRRQVFVQTDTGCVLG 60
           MSR LDSPVQTQMAVA  K+PL+ A + +     K      Q  GRR VFVQT+TGCVLG
Sbjct: 1   MSRNLDSPVQTQMAVAVFKTPLTGASKMEGKQHHKHQHLQRQSSGRR-VFVQTETGCVLG 60

Query: 61  MELDRSDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSS 120
           MELDRSDN HTVKRRLQ+ALN PTEESSLT+GD++L NDLSA+RNDSPLLL RN +HRSS
Sbjct: 61  MELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSAVRNDSPLLLKRNFMHRSS 120

Query: 121 STPCLSPTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGA 180
           STPCLSPTG+DL+ +D+SGPIEILGH D F+  + +VK+IVK +K+G++P+PVHSGLGGA
Sbjct: 121 STPCLSPTGRDLQQKDRSGPIEILGHSDCFSIVKHMVKDIVKAMKMGVEPLPVHSGLGGA 180

Query: 181 YYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLD 240
           YYFRN +GESVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLD
Sbjct: 181 YYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVRVGETGFREVAAYLLD 240

Query: 241 YEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGT 300
           Y  FANVPPTALVKITHS+FNVND V GNKP+  +KK VSKIASFQ+F+ HDFDASDHGT
Sbjct: 241 YGRFANVPPTALVKITHSVFNVNDGVKGNKPR--EKKLVSKIASFQKFVAHDFDASDHGT 300

Query: 301 SSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDP 360
           SSFPV +VHRIGILDIRIFNTDRH GNLLV+KLDG+G FGQVELIPIDHGLCLPETLEDP
Sbjct: 301 SSFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDP 360

Query: 361 YFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAA 420
           YFEWIHWPQAS+PFSD+E+ YI++LDP+KDC+MLR ELPMIREACLRVLVLCTIFLKEA+
Sbjct: 361 YFEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEAS 420

Query: 421 ASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYE-WGNEDFQFDL 480
           A GLCLAEIGEMM+REFR GEEEPSELE+VC+EA++ + ER+  SP  +  G  +FQFDL
Sbjct: 421 AYGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDL 480

Query: 481 DCEDGELDYNSSM-AADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEE-- 540
           DC+D E  Y+S +   D     + +         +GR+ L KLEESI+E  ED   EE  
Sbjct: 481 DCDDLESVYSSKIQLTDDYFTKNPFS--------NGRSSLGKLEESIKEEEEDEEEEEDK 540

Query: 541 -------------LGETGIFPPSEPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYI 600
                           +  F    P S+SKLS S+KNT L +  +KN K  +T+   G  
Sbjct: 541 TENTVPMIIMKDSFFSSAAFHDKAP-SLSKLSTSMKNTHLSDTTRKNPK-PLTR---GKS 600

Query: 601 TNTSAGHRSANEQFPPSVSFVKLGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQ 646
            NTS+GH+SANEQ P S SFVK+ DM E++W LFL++F ELL PAFAKRK+A L   +RQ
Sbjct: 601 ENTSSGHKSANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQ 650

BLAST of Carg01775 vs. TAIR 10
Match: AT1G26270.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 861.7 bits (2225), Expect = 3.8e-250
Identity = 441/647 (68.16%), Postives = 517/647 (79.91%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEKQPVGRRQVFVQTDTGCVLGMELDRS 60
           MSRKLDSP++TQMAVA +KSPL+  +R  N +  K PVGRR+VFVQT+TGCVLG+ELDRS
Sbjct: 1   MSRKLDSPIKTQMAVALVKSPLNGEFREFNKVGMKTPVGRRRVFVQTETGCVLGLELDRS 60

Query: 61  DNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLS 120
           DNAHTVKR+LQ+ALN P EESSLTFGD++LKNDL+A+R+DSPLLLTRN  HRSSSTPCLS
Sbjct: 61  DNAHTVKRKLQVALNFPIEESSLTFGDLVLKNDLTAVRSDSPLLLTRNNFHRSSSTPCLS 120

Query: 121 PTGKDL-RLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRN 180
           P   DL + RD+S PIEILG+   F+  R++ K+I K +K GIDP+ V+SGLGGAYYF+N
Sbjct: 121 PMRADLQQRRDESSPIEILGNSVSFSFVRQMAKDITKAVKKGIDPVAVNSGLGGAYYFKN 180

Query: 181 GKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFA 240
            +GESVAIVKPTDEEP+APNNPKGFVGKALGQPG KRSVRVGETG+REVAAYLLD EHFA
Sbjct: 181 SRGESVAIVKPTDEEPYAPNNPKGFVGKALGQPGLKRSVRVGETGYREVAAYLLDKEHFA 240

Query: 241 NVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPV 300
           NVPPTALVKITHSIFNVND V  +KP   +K  VSKIAS QQFIPHD+DAS+HGTS+FPV
Sbjct: 241 NVPPTALVKITHSIFNVNDGVKASKPM--EKMLVSKIASLQQFIPHDYDASEHGTSNFPV 300

Query: 301 AAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDPYFEWI 360
           +AVHRIGILDIRI NTDRH+GNLLV+KLDG G FGQVEL+PIDHGLCLPETLEDPYFEWI
Sbjct: 301 SAVHRIGILDIRILNTDRHSGNLLVKKLDGDGMFGQVELVPIDHGLCLPETLEDPYFEWI 360

Query: 361 HWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLC 420
           HWPQASIPFS+DELKYI NLDP+ DCEMLR ELPM+REA LRVLVLCTIFLKEAAA+GLC
Sbjct: 361 HWPQASIPFSEDELKYIANLDPLGDCEMLRRELPMVREASLRVLVLCTIFLKEAAANGLC 420

Query: 421 LAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEWGNE-DFQFDLDCEDG 480
           LAEIGEMM+RE R G+EEPSE+E+VCLEA  +I E++  SP  + GN+ +FQFD+DCE+ 
Sbjct: 421 LAEIGEMMTREVRPGDEEPSEIEVVCLEAMSLIGEKDAESPRSDLGNDIEFQFDIDCEE- 480

Query: 481 ELDYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFP 540
                       C       LG   G   G+  LSK+EE+ E+G E+   +   E     
Sbjct: 481 ---------VTDCTKKLALPLGLTFGNARGQ--LSKVEETTEDGEEEEEEDREEEENDRA 540

Query: 541 PSEPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFV 600
             E +   KLSMSLK+T+LG K+QK QK+   + E  Y    S+ HRSA+EQ P S SFV
Sbjct: 541 DLEKMPTIKLSMSLKSTLLGEKSQKYQKHPGARVESAY---ASSAHRSADEQIPSSTSFV 600

Query: 601 KLGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
           KL DM+EE+W++FL+K+ ELL PA  KRKS  LG +QRQRLGTSCQF
Sbjct: 601 KLSDMSEEEWTIFLEKYQELLYPAIEKRKSITLGQKQRQRLGTSCQF 630

BLAST of Carg01775 vs. TAIR 10
Match: AT1G13640.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 804.3 bits (2076), Expect = 7.2e-233
Identity = 412/645 (63.88%), Postives = 492/645 (76.28%), Query Frame = 0

Query: 13  MAVAALKSPLSRAYRSKNGME---------EKQPVGRRQVFVQTDTGCVLGMELDRSDNA 72
           MA+A  K+PL   +     ME         ++Q  GRR+VFVQTDTGCVLG+ELDR+DN 
Sbjct: 1   MAMAVFKAPLKGEFHGARKMEGKQYKHHLLQQQSTGRRRVFVQTDTGCVLGVELDRNDNV 60

Query: 73  HTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSSSTPCLSPTG 132
           HTVK+RLQ+A N PTEESSLTFGD++LKNDLSA+RNDSPLLL RNL+HRSSSTPCLSPTG
Sbjct: 61  HTVKKRLQIAFNFPTEESSLTFGDMVLKNDLSAVRNDSPLLLKRNLMHRSSSTPCLSPTG 120

Query: 133 KDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGAYYFRNGKGE 192
            DL+ +D+SGPIEIL H   F   ++   +IVK +K+G++PIPV+ GLGGAYYFR+ KG+
Sbjct: 121 NDLQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRDEKGQ 180

Query: 193 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLDYEHFANVPP 252
           SVAIVKPTDEEPFAPNNPKGFVGKALGQPG K SVRVGETGFREVAAYLLDY+HFANVPP
Sbjct: 181 SVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDYDHFANVPP 240

Query: 253 TALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGTSSFPVAAVH 312
           TALVKITHS+FNVND ++GNK +  KK   SKIASFQ+F+PHDFDASDHGTSSFPVA+VH
Sbjct: 241 TALVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHDFDASDHGTSSFPVASVH 300

Query: 313 RIGILDIRIFNTDRHAGNLLVRKLD--GIGSFGQVELIPIDHGLCLPETLEDPYFEWIHW 372
           RIGILDIRI NTDRH GNLLV+KLD  G+G FGQVELIPIDHGLCLPETLEDPYFEWIHW
Sbjct: 301 RIGILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHW 360

Query: 373 PQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAAASGLCLA 432
           PQASIPFS++EL YI++LDP+KDCEMLR ELPMIREACLRVLVLCT+FLKEAA  GLCLA
Sbjct: 361 PQASIPFSEEELDYIQSLDPVKDCEMLRRELPMIREACLRVLVLCTVFLKEAAVFGLCLA 420

Query: 433 EIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYEW-GNEDFQFDLDCEDGEL 492
           EIGEMM+REFR+GEEEPSELE++C+EA+++  E++  SP  +  G  +FQFD+D  + + 
Sbjct: 421 EIGEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPKSDGEGETEFQFDIDYNELDS 480

Query: 493 DYNSSMAADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEELGETGIFPPS 552
            Y S    D+    + +         +GR+ L +L+ESI E  ED   E           
Sbjct: 481 VYGSETETDEFFAKNPFS--------NGRSSLGELKESIAEEEEDDEEE---------AK 540

Query: 553 EPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKL 612
             +S+SKLS S+KN +    N     Y +  P+          H+SAN Q P SV+FVKL
Sbjct: 541 LTLSLSKLSTSMKNNL---SNTMGSGY-LKPPKDNQTDKALVSHKSANVQLPLSVNFVKL 600

Query: 613 GDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQRLGTSCQF 646
            DM E +W +FL++F ELL  AFA+RK+  L  R  QRLGTSC+F
Sbjct: 601 ADMKEVEWVVFLERFQELLYSAFAERKTMTL--RNTQRLGTSCKF 622

BLAST of Carg01775 vs. TAIR 10
Match: AT2G03890.2 (phosphoinositide 4-kinase gamma 7 )

HSP 1 Score: 619.0 bits (1595), Expect = 4.3e-177
Identity = 361/668 (54.04%), Postives = 420/668 (62.87%), Query Frame = 0

Query: 1   MSRKLDSPVQTQMAVAALKSPLSRAYRSKNGMEEK------QPVGRRQVFVQTDTGCVLG 60
           MSR LDSPVQTQMAVA  K+PL+ A + +     K      Q  GRR VFVQT+TGCVLG
Sbjct: 1   MSRNLDSPVQTQMAVAVFKTPLTGASKMEGKQHHKHQHLQRQSSGRR-VFVQTETGCVLG 60

Query: 61  MELDRSDNAHTVKRRLQLALNIPTEESSLTFGDVILKNDLSAIRNDSPLLLTRNLLHRSS 120
           MELDRSDN HTVKRRLQ+ALN PTEESSLT+GD++L NDLS+                  
Sbjct: 61  MELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSS------------------ 120

Query: 121 STPCLSPTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPIPVHSGLGGA 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 YYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGFREVAAYLLD 240
                                                     SVRVGETGFREVAAYLLD
Sbjct: 181 ------------------------------------------SVRVGETGFREVAAYLLD 240

Query: 241 YEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPHDFDASDHGT 300
           Y  FANVPPTALVKITHS+FNVND V GNKP+  +KK VSKIASFQ+F+ HDFDASDHGT
Sbjct: 241 YGRFANVPPTALVKITHSVFNVNDGVKGNKPR--EKKLVSKIASFQKFVAHDFDASDHGT 300

Query: 301 SSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDGIGSFGQVELIPIDHGLCLPETLEDP 360
           SSFPV +VHRIGILDIRIFNTDRH GNLLV+KLDG+G FGQVELIPIDHGLCLPETLEDP
Sbjct: 301 SSFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDP 360

Query: 361 YFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREACLRVLVLCTIFLKEAA 420
           YFEWIHWPQAS+PFSD+E+ YI++LDP+KDC+MLR ELPMIREACLRVLVLCTIFLKEA+
Sbjct: 361 YFEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEAS 420

Query: 421 ASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSSPIYE-WGNEDFQFDL 480
           A GLCLAEIGEMM+REFR GEEEPSELE+VC+EA++ + ER+  SP  +  G  +FQFDL
Sbjct: 421 AYGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDL 480

Query: 481 DCEDGELDYNSSM-AADQCLGWSHYQLGGLVGGLHGRNPLSKLEESIEEGGEDSGGEE-- 540
           DC+D E  Y+S +   D     + +         +GR+ L KLEESI+E  ED   EE  
Sbjct: 481 DCDDLESVYSSKIQLTDDYFTKNPFS--------NGRSSLGKLEESIKEEEEDEEEEEDK 530

Query: 541 -------------LGETGIFPPSEPISVSKLSMSLKNTILGNKNQKNQKYQVTKPEFGYI 600
                           +  F    P S+SKLS S+KNT L +  +KN K  +T+   G  
Sbjct: 541 TENTVPMIIMKDSFFSSAAFHDKAP-SLSKLSTSMKNTHLSDTTRKNPK-PLTR---GKS 530

Query: 601 TNTSAGHRSANEQFPPSVSFVKLGDMNEEDWSLFLDKFPELLRPAFAKRKSAALGPRQRQ 646
            NTS+GH+SANEQ P S SFVK+ DM E++W LFL++F ELL PAFAKRK+A L   +RQ
Sbjct: 601 ENTSSGHKSANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQ 530

BLAST of Carg01775 vs. TAIR 10
Match: AT3G56600.1 (Protein kinase superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 6.5e-101
Identity = 233/559 (41.68%), Postives = 307/559 (54.92%), Query Frame = 0

Query: 106 TRNLLHRSSSTPCLSPTGKDLRLRDQSGPIEILGHPDHFAGTRRLVKEIVKGIKLGIDPI 165
           + +L +RS STPC S +G +L     +  IEILG        R LV E+   I  G  P+
Sbjct: 51  SHSLNNRSVSTPCFSISGSNLD-GSATPHIEILG-GQRVPTVRSLVAEVTIAIVSGAQPL 110

Query: 166 PVHSGLGGAYYFRNGKGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGFKRSVRVGETGF 225
            + SGLGGAY  +  KG ++A+ KP DEEP A NNPKG  G  LGQPG KRS+RVGE+G 
Sbjct: 111 LLPSGLGGAYLLQTEKGNNIAVAKPVDEEPLAFNNPKGSGGLTLGQPGMKRSIRVGESGI 170

Query: 226 REVAAYLLDYEHFANVPPTALVKITHSIFNVNDWVNGNKPQAHKKKPVSKIASFQQFIPH 285
           RE+AAYLLD++ F++VPPTALV+I+H  F+      G+   A+      K+AS Q+F+ H
Sbjct: 171 RELAAYLLDHQGFSSVPPTALVRISHVPFH----DRGSDHAAY------KVASLQRFVGH 230

Query: 286 DFDASDHGTSSFPVAAVHRIGILDIRIFNTDRHAGNLLVRKLDG------IGSFGQVELI 345
           DFDA + G  SF V +VHRIGILD+R+ N DRHAGN+LV+K+            G  EL+
Sbjct: 231 DFDAGELGPGSFTVVSVHRIGILDVRVLNLDRHAGNMLVKKIHDQDETTCSNGVGAAELV 290

Query: 346 PIDHGLCLPETLEDPYFEWIHWPQASIPFSDDELKYIENLDPIKDCEMLRMELPMIREAC 405
           PIDHGLCLPE L+DPYFEW++WPQAS+PF+D EL+YI NLDP KD E+LR EL  I+E+ 
Sbjct: 291 PIDHGLCLPECLDDPYFEWLNWPQASVPFTDIELQYISNLDPFKDAELLRTELDSIQESS 350

Query: 406 LRVLVLCTIFLKEAAASGLCLAEIGEMMSREFRSGEEEPSELELVCLEARQMIAEREFSS 465
           LRVL++CTIFLKEAAA+GL LAEIGE M+R+   GEE  S LE++C +A+          
Sbjct: 351 LRVLIVCTIFLKEAAAAGLSLAEIGEKMTRDICRGEESSSVLEILCNKAKASAVSGSDDD 410

Query: 466 PIY--EWGNED-------FQFDLDCEDGELDYNSSMAADQCLGWSHYQLGGLVGGLHGRN 525
             Y  EW   +       FQFD + E  EL     +     +      L  L+     R 
Sbjct: 411 DDYSSEWNEVEAELECGIFQFDDEVECKELPDMLQVPIFTRVPSIAANLSALM-----RC 470

Query: 526 P----LSKLEESIEEGGEDSGGEELGETGIFPPSEPISVSKLSMSLKNTILGNKNQKNQK 585
           P    +S  + +IEE       E    + +   S PI V             N ++K   
Sbjct: 471 PPNQWISTYDTNIEE-------ERRDRSIVRSKSHPICV-------------NYDEKEGV 530

Query: 586 YQVTKPEFGYITNTSAGHRSANEQFPPSVSFVKLGDMNEEDWSLFLDKFPELLRPAFAKR 645
           Y                                 GDM+ ++W +FL  F  LL  A    
Sbjct: 531 Y--------------------------------FGDMSGDEWEMFLHSFQMLLPEAL--E 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6601359.10.0e+00100.00Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022956825.10.0e+0099.22phosphatidylinositol 4-kinase gamma 5-like [Cucurbita moschata] >XP_022956826.1 ... [more]
XP_023533870.10.0e+0099.22phosphatidylinositol 4-kinase gamma 5-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022976415.10.0e+0099.07phosphatidylinositol 4-kinase gamma 5-like [Cucurbita maxima] >XP_022976424.1 ph... [more]
XP_008446479.10.0e+0094.74PREDICTED: phosphatidylinositol 4-kinase gamma 5 [Cucumis melo] >XP_008446480.1 ... [more]
Match NameE-valueIdentityDescription
Q9SI521.5e-25167.96Phosphatidylinositol 4-kinase gamma 7 OS=Arabidopsis thaliana OX=3702 GN=PI4KG7 ... [more]
Q9C6715.3e-24968.16Phosphatidylinositol 4-kinase gamma 5 OS=Arabidopsis thaliana OX=3702 GN=PI4KG5 ... [more]
Q8W4R81.0e-23163.88Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 ... [more]
Q0WMZ69.2e-10041.68Phosphatidylinositol 4-kinase gamma 8 OS=Arabidopsis thaliana OX=3702 GN=PI4KG8 ... [more]
O221991.3e-9849.87Phosphatidylinositol 4-kinase gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KG1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GYU50.0e+0099.221-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111458409 PE... [more]
A0A6J1IJE70.0e+0099.071-phosphatidylinositol 4-kinase OS=Cucurbita maxima OX=3661 GN=LOC111476826 PE=3... [more]
A0A1S3BFU20.0e+0094.741-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103489203 PE=3 SV=... [more]
A0A5D3CB140.0e+0094.741-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KWA50.0e+0093.961-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G606910 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G03890.11.1e-25267.96phosphoinositide 4-kinase gamma 7 [more]
AT1G26270.13.8e-25068.16Phosphatidylinositol 3- and 4-kinase family protein [more]
AT1G13640.17.2e-23363.88Phosphatidylinositol 3- and 4-kinase family protein [more]
AT2G03890.24.3e-17754.04phosphoinositide 4-kinase gamma 7 [more]
AT3G56600.16.5e-10141.68Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainPFAMPF00454PI3_PI4_kinasecoord: 171..423
e-value: 6.0E-50
score: 170.4
NoneNo IPR availablePANTHERPTHR45800:SF11PHOSPHATIDYLINOSITOL 4-KINASE GAMMA 7coord: 6..645
NoneNo IPR availablePANTHERPTHR45800:SF18PHOSPHATIDYLINOSITOL 4-KINASE GAMMA 7-LIKE ISOFORM X1coord: 6..645
NoneNo IPR availableCDDcd17039Ubl_ubiquitin_likecoord: 43..105
e-value: 7.26953E-4
score: 36.4202
IPR044571Phosphatidylinositol 4-kinase gamma 1-8PANTHERPTHR45800PHOSPHATIDYLINOSITOL 4-KINASE GAMMAcoord: 6..645
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 41..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01775-RACarg01775-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
biological_process GO:0016310 phosphorylation
molecular_function GO:0004430 1-phosphatidylinositol 4-kinase activity