Carg01737 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01737
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptiondnaJ homolog subfamily B member 4-like
LocationCarg_Chr04: 9564903 .. 9566634 (+)
RNA-Seq ExpressionCarg01737
SyntenyCarg01737
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGTGGACTACTACAATGTGTTGAAGGTGAACAGAAATGCCAATGAAGAGGACCTGAAGAGGTCCTACAAGAGTTTGGCTATGAAATGGCATCCTGATAAGAATCCTTCCAACAAGAAAGAGGCGGAGGCAAAGTTCAAGCAGATCTCTGAGGCTTATGATGTTTTAAGCGACGCTAAGAAGCGTCAGATCTACGATCTCTACGGTGAGGAGGCTCTCAATTCCGCCGATTTTGTTCCTCCGCCCGATTCCAATCCCTCTTTTTCTTATTTCCCTCGCGATGCCGATGACATTTTTGCCGAGTTTTTTGGCGGAGCTGGGAGTGCTAAGAGCGGAGGGTTTAAGAGAGAGAGTTTGTTTAGGAATGGGAAGGCGGAGGCTGGGAAGAAATCGAATAGGAAAGCACCGCCAATTGAGAGCAAGTTAATGTGTAGCTTGGAGGAGATTTACAAAGGTTCGCGAAGAAAGATGAGGATTTCCCACACTGTCCCCGATGAATTTGGGTATGTAAAATTTCTTGTTTACTTTTTCTGTTTTTGATGAAACAAAACGTTAAATGCTTATAGCTCTAATTTGTTTCGATTGCATCTAATGGTGTCGATGAATGTCCATGGGTTACTAGTAGTGATCTTTGATGCTGTAAAACTTAAGGGGTGGAGATGTAGAGGAAAATATTATTCCTTTTTCCTGGCTGAGCTGCCTTGGATTATCAGGATATTGATATCCCTTTACATGGCAAATGATTCCTTCTGTTCTACTTTTACCATTGATAGAAAGGCGACAGGATGAACAATTATGAACCAGTGGTGTGCAACTCTGAAAATAGAATAGCATATTATTTCGAACTCTTATCTACCTAAGCTGTGAAGCCTACTTGTGTAGCTAATTGGATGGTTGTGCATGTGTTGCAGAAAGACAATGGGTAGATTATGATCTTTTGAATATTATACTCTAACATAAAGTTTATGATCAGGAAAGGACTTGGTGCTTCTACCTTCAATAACCACAAGGCTGCCCTTTTTTATAACTTCCAGCTGACTAAAGCATCTATAAGGATATCTTCTGTTATTTAGGAGTAATAAGTATGCTTTTATTGCTGAATTGGAAAGCAATATTGATTTGGATTAGATGAACTTAAGCTTGTTCTTTCTTTCTTGCATCACATTGCAATCAAGCTCACAATTGACACCTTATTTCATTGATCTCATTGTATCTTCAATGTTCTTTCATTTTCTTCTTCACTTTTTCCTCCTCAATTTAGGCTTATTCAAGGAACTTGTTTTGTAGGAAGCCAAAAACTGTTGATGAAGTGTTAAAAATAGATATTAAACCTGGTTGGAAGAAGGGGACAAAAGTCACTTTCCCTGAGAAAGGCAATCAAGAACCTGGAGTTTCTCCTGCTGATCTTATATTTATCATCGATGAGAAACCCCATCCTGTATTTGAGAGGGATGGTAATGATTTGGTAATCAATCAGAAAATATCTCTATTAGAAGCACTCACTGGAAAGACACTGAACGTTACAACTTTGGATGGAAGAAATCTCACTTCTGTAACTGATATTGTCAAACCCGGTTACGAGGTAGTGATCCAGAATGAAGGAATGCCCATCTCTAAAGAACCAAACAAGAAGGGGAACCTAAGGATCAAGTTTGATATCATCTTCCCATCAATGCTTACTTTTGAACAGAAATCTGATCTAAAAAGGGCTTTGGGTGGTTCTGATTAA

mRNA sequence

ATGGGGGTGGACTACTACAATGTGTTGAAGGTGAACAGAAATGCCAATGAAGAGGACCTGAAGAGGTCCTACAAGAGTTTGGCTATGAAATGGCATCCTGATAAGAATCCTTCCAACAAGAAAGAGGCGGAGGCAAAGTTCAAGCAGATCTCTGAGGCTTATGATGTTTTAAGCGACGCTAAGAAGCGTCAGATCTACGATCTCTACGGTGAGGAGGCTCTCAATTCCGCCGATTTTGTTCCTCCGCCCGATTCCAATCCCTCTTTTTCTTATTTCCCTCGCGATGCCGATGACATTTTTGCCGAGTTTTTTGGCGGAGCTGGGAGTGCTAAGAGCGGAGGGTTTAAGAGAGAGAGTTTGTTTAGGAATGGGAAGGCGGAGGCTGGGAAGAAATCGAATAGGAAAGCACCGCCAATTGAGAGCAAGTTAATGTGTAGCTTGGAGGAGATTTACAAAGGTTCGCGAAGAAAGATGAGGATTTCCCACACTGTCCCCGATGAATTTGGGAAGCCAAAAACTGTTGATGAAGTGTTAAAAATAGATATTAAACCTGGTTGGAAGAAGGGGACAAAAGTCACTTTCCCTGAGAAAGGCAATCAAGAACCTGGAGTTTCTCCTGCTGATCTTATATTTATCATCGATGAGAAACCCCATCCTGTATTTGAGAGGGATGGTAATGATTTGGTAATCAATCAGAAAATATCTCTATTAGAAGCACTCACTGGAAAGACACTGAACGTTACAACTTTGGATGGAAGAAATCTCACTTCTGTAACTGATATTGTCAAACCCGGTTACGAGGTAGTGATCCAGAATGAAGGAATGCCCATCTCTAAAGAACCAAACAAGAAGGGGAACCTAAGGATCAAGTTTGATATCATCTTCCCATCAATGCTTACTTTTGAACAGAAATCTGATCTAAAAAGGGCTTTGGGTGGTTCTGATTAA

Coding sequence (CDS)

ATGGGGGTGGACTACTACAATGTGTTGAAGGTGAACAGAAATGCCAATGAAGAGGACCTGAAGAGGTCCTACAAGAGTTTGGCTATGAAATGGCATCCTGATAAGAATCCTTCCAACAAGAAAGAGGCGGAGGCAAAGTTCAAGCAGATCTCTGAGGCTTATGATGTTTTAAGCGACGCTAAGAAGCGTCAGATCTACGATCTCTACGGTGAGGAGGCTCTCAATTCCGCCGATTTTGTTCCTCCGCCCGATTCCAATCCCTCTTTTTCTTATTTCCCTCGCGATGCCGATGACATTTTTGCCGAGTTTTTTGGCGGAGCTGGGAGTGCTAAGAGCGGAGGGTTTAAGAGAGAGAGTTTGTTTAGGAATGGGAAGGCGGAGGCTGGGAAGAAATCGAATAGGAAAGCACCGCCAATTGAGAGCAAGTTAATGTGTAGCTTGGAGGAGATTTACAAAGGTTCGCGAAGAAAGATGAGGATTTCCCACACTGTCCCCGATGAATTTGGGAAGCCAAAAACTGTTGATGAAGTGTTAAAAATAGATATTAAACCTGGTTGGAAGAAGGGGACAAAAGTCACTTTCCCTGAGAAAGGCAATCAAGAACCTGGAGTTTCTCCTGCTGATCTTATATTTATCATCGATGAGAAACCCCATCCTGTATTTGAGAGGGATGGTAATGATTTGGTAATCAATCAGAAAATATCTCTATTAGAAGCACTCACTGGAAAGACACTGAACGTTACAACTTTGGATGGAAGAAATCTCACTTCTGTAACTGATATTGTCAAACCCGGTTACGAGGTAGTGATCCAGAATGAAGGAATGCCCATCTCTAAAGAACCAAACAAGAAGGGGAACCTAAGGATCAAGTTTGATATCATCTTCCCATCAATGCTTACTTTTGAACAGAAATCTGATCTAAAAAGGGCTTTGGGTGGTTCTGATTAA

Protein sequence

MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDAKKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESLFRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRALGGSD
Homology
BLAST of Carg01737 vs. NCBI nr
Match: KAG7032180.1 (DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 628.2 bits (1619), Expect = 3.7e-176
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDLKRALGGSD
Sbjct: 301 FEQKSDLKRALGGSD 315

BLAST of Carg01737 vs. NCBI nr
Match: KAG6601400.1 (DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 625.2 bits (1611), Expect = 3.1e-175
Identity = 313/315 (99.37%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKV+RNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVSRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 301 FEQKSDLRRALGGSD 315

BLAST of Carg01737 vs. NCBI nr
Match: XP_023537929.1 (dnaJ homolog subfamily B member 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624.0 bits (1608), Expect = 7.0e-175
Identity = 313/315 (99.37%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRIS TVPDEFGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISRTVPDEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 301 FEQKSDLRRALGGSD 315

BLAST of Carg01737 vs. NCBI nr
Match: XP_022957391.1 (dnaJ homolog subfamily B member 4-like [Cucurbita moschata])

HSP 1 Score: 622.5 bits (1604), Expect = 2.0e-174
Identity = 312/315 (99.05%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDA+DIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDAEDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRIS TVPDEFGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISRTVPDEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 301 FEQKSDLRRALGGSD 315

BLAST of Carg01737 vs. NCBI nr
Match: XP_022999795.1 (dnaJ homolog subfamily B member 4-like [Cucurbita maxima])

HSP 1 Score: 620.5 bits (1599), Expect = 7.7e-174
Identity = 311/315 (98.73%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRIS TVPD+FGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISRTVPDDFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGG D
Sbjct: 301 FEQKSDLRRALGGFD 315

BLAST of Carg01737 vs. ExPASy Swiss-Prot
Match: Q9D832 (DnaJ homolog subfamily B member 4 OS=Mus musculus OX=10090 GN=Dnajb4 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.0e-63
Identity = 140/339 (41.30%), Postives = 204/339 (60.18%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MG DYY++L +++ A +ED+K++Y+  A+K+HPDKN S   +AE KFK+++EAY+VLSD 
Sbjct: 1   MGKDYYHILGIDKGATDEDVKKAYRKQALKFHPDKNKS--PQAEEKFKEVAEAYEVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGA-------GSAKSG 120
           KKR+IYD +GEE L                 F  D    FA FFGG+       G    G
Sbjct: 61  KKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGG 120

Query: 121 GFKRESLFRNG----------------KAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRK 180
           G   E +  +G                +   G    ++ PPI  +L  SLEEIY G  ++
Sbjct: 121 GRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPIIHELKVSLEEIYSGCTKR 180

Query: 181 MRISHTVPDEFGKP-KTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEK 240
           M+IS    +  G+  ++ D++L I+IK GWK+GTK+TFP +G++ P   PAD++F+I +K
Sbjct: 181 MKISRKRLNPDGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFVIKDK 240

Query: 241 PHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNL-TSVTDIVKPGYEVVIQNEGM 300
            HP F+RDG+++V   KISL EAL G +LNV T+DGRNL  SVTDIVKPG    +   G+
Sbjct: 241 EHPKFKRDGSNIVYTAKISLREALCGCSLNVPTMDGRNLPMSVTDIVKPGMRRRVIGYGL 300

Query: 301 PISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRALGGS 315
           P  K P+++G+L I+FD+ FP +++   K  L++ L  S
Sbjct: 301 PFPKNPDQRGDLLIEFDVSFPDVISAASKEILRKHLPAS 337

BLAST of Carg01737 vs. ExPASy Swiss-Prot
Match: P25685 (DnaJ homolog subfamily B member 1 OS=Homo sapiens OX=9606 GN=DNAJB1 PE=1 SV=4)

HSP 1 Score: 239.2 bits (609), Expect = 6.4e-62
Identity = 139/343 (40.52%), Postives = 207/343 (60.35%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MG DYY  L + R A++E++KR+Y+  A+++HPDKN   +  AE KFK+I+EAYDVLSD 
Sbjct: 1   MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKN--KEPGAEEKFKEIAEAYDVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDS-----NPSFSY-FPRDADDIFAEFFGGAGSAKS-- 120
           +KR+I+D YGEE L  +   P   S       SFSY F  D   +FAEFFGG     +  
Sbjct: 61  RKREIFDRYGEEGLKGSG--PSGGSGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFF 120

Query: 121 ----------------------GGFKRESLFRNGKAEAGKKSNRKAPPIESKLMCSLEEI 180
                                 GGF   +  R+  A+   +  ++ PP+   L  SLEEI
Sbjct: 121 GQRNGEEGMDIDDPFSGFPMGMGGFTNVNFGRSRSAQEPAR-KKQDPPVTHDLRVSLEEI 180

Query: 181 YKGSRRKMRISHTVPDEFGKP-KTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADL 240
           Y G  +KM+ISH   +  GK  +  D++L I++K GWK+GTK+TFP++G+Q     PAD+
Sbjct: 181 YSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKKGWKEGTKITFPKEGDQTSNNIPADI 240

Query: 241 IFIIDEKPHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNLTSV-TDIVKPGYEV 300
           +F++ +KPH +F+RDG+D++   +ISL EAL G T+NV TLDGR +  V  D+++PG   
Sbjct: 241 VFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRR 300

Query: 301 VIQNEGMPISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRAL 312
            +  EG+P+ K P K+G+L I+F++IFP  +    ++ L++ L
Sbjct: 301 KVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVL 338

BLAST of Carg01737 vs. ExPASy Swiss-Prot
Match: Q5R8J8 (DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.4e-62
Identity = 137/339 (40.41%), Postives = 201/339 (59.29%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MG DYY +L + + A++ED+K++Y+  A+K+HPDKN S   +AE KFK+++EAY+VLSD 
Sbjct: 1   MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKS--PQAEEKFKEVAEAYEVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGA-------GSAKSG 120
           KKR+IYD +GEE L                 F  D    FA FFGG+       G    G
Sbjct: 61  KKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGG 120

Query: 121 GFKRESLFRNG----------------KAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRK 180
           G   E +  +G                +   G    ++ PP+  +L  SLEEIY G  ++
Sbjct: 121 GRDSEEMEMDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKR 180

Query: 181 MRISHTVPDEFGKP-KTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEK 240
           M+IS    +  G+  ++ D++L I+IK GWK+GTK+TFP +G++ P   PAD++FII +K
Sbjct: 181 MKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDK 240

Query: 241 PHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNL-TSVTDIVKPGYEVVIQNEGM 300
            HP F+RDG++++   KISL EAL G ++NV TLDGRN+  SV DIVKPG    I   G+
Sbjct: 241 DHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGL 300

Query: 301 PISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRALGGS 315
           P  K P+++G+L I+F++ FP  ++   K  L++ L  S
Sbjct: 301 PFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS 337

BLAST of Carg01737 vs. ExPASy Swiss-Prot
Match: Q3MI00 (DnaJ homolog subfamily B member 1 OS=Bos taurus OX=9913 GN=DNAJB1 PE=2 SV=3)

HSP 1 Score: 238.8 bits (608), Expect = 8.3e-62
Identity = 139/343 (40.52%), Postives = 208/343 (60.64%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MG DYY  L + R A++E++KR+Y+  A+++HPDKN   +  AE KFK+I+EAYDVLSD 
Sbjct: 1   MGKDYYQTLGLARGASDEEIKRAYRRQALRYHPDKN--KEPGAEEKFKEIAEAYDVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSN-----PSFSY-FPRDADDIFAEFFGGAGSAKS-- 120
           +KR+I+D YGEE L  +   P   S+      SFSY F  D   +FAEFFGG     +  
Sbjct: 61  RKREIFDRYGEEGLKGSG--PSGGSSGGTNGTSFSYTFHGDPHAMFAEFFGGRNPFDNFF 120

Query: 121 ----------------------GGFKRESLFRNGKAEAGKKSNRKAPPIESKLMCSLEEI 180
                                 GGF   +  R+  A+   +  ++ PP+   L  SLEEI
Sbjct: 121 GQRNGEEGMDIDDPFSGFPMGMGGFTNMNFGRSRPAQEPTR-KKQDPPVTHDLRVSLEEI 180

Query: 181 YKGSRRKMRISHTVPDEFGKP-KTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADL 240
           Y G  +KM+ISH   +  GK  +  D++L I++K GWK+GTK+TFP++G+Q     PAD+
Sbjct: 181 YSGCTKKMKISHKRLNPDGKSIRNEDKILTIEVKRGWKEGTKITFPKEGDQTSNNIPADI 240

Query: 241 IFIIDEKPHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNLTSV-TDIVKPGYEV 300
           +F++ +KPH +F+RDG+D++   +ISL EAL G T+NV TLDGR +  V  D+++PG   
Sbjct: 241 VFVLKDKPHNIFKRDGSDVIYPARISLREALCGCTVNVPTLDGRTIPVVFKDVIRPGMRR 300

Query: 301 VIQNEGMPISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRAL 312
            +  EG+P+ K P K+G+L I+F++IFP  +    ++ L++ L
Sbjct: 301 KVPGEGLPLPKTPEKRGDLIIEFEVIFPERIPQTSRTVLEQVL 338

BLAST of Carg01737 vs. ExPASy Swiss-Prot
Match: Q9UDY4 (DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.1e-61
Identity = 137/339 (40.41%), Postives = 201/339 (59.29%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MG DYY +L + + A++ED+K++Y+  A+K+HPDKN S   +AE KFK+++EAY+VLSD 
Sbjct: 1   MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKS--PQAEEKFKEVAEAYEVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGA-------GSAKSG 120
           KKR+IYD +GEE L                 F  D    FA FFGG+       G    G
Sbjct: 61  KKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGG 120

Query: 121 GFKRESLFRNG----------------KAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRK 180
           G   E +  +G                +   G    ++ PP+  +L  SLEEIY G  ++
Sbjct: 121 GRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKR 180

Query: 181 MRISHTVPDEFGKP-KTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEK 240
           M+IS    +  G+  ++ D++L I+IK GWK+GTK+TFP +G++ P   PAD++FII +K
Sbjct: 181 MKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDK 240

Query: 241 PHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNL-TSVTDIVKPGYEVVIQNEGM 300
            HP F+RDG++++   KISL EAL G ++NV TLDGRN+  SV DIVKPG    I   G+
Sbjct: 241 DHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGL 300

Query: 301 PISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRALGGS 315
           P  K P+++G+L I+F++ FP  ++   K  L++ L  S
Sbjct: 301 PFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS 337

BLAST of Carg01737 vs. ExPASy TrEMBL
Match: A0A6J1GZ30 (dnaJ homolog subfamily B member 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458803 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 9.8e-175
Identity = 312/315 (99.05%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDA+DIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDAEDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRIS TVPDEFGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISRTVPDEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 301 FEQKSDLRRALGGSD 315

BLAST of Carg01737 vs. ExPASy TrEMBL
Match: A0A6J1KGL1 (dnaJ homolog subfamily B member 4-like OS=Cucurbita maxima OX=3661 GN=LOC111494096 PE=4 SV=1)

HSP 1 Score: 620.5 bits (1599), Expect = 3.7e-174
Identity = 311/315 (98.73%), Postives = 313/315 (99.37%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL
Sbjct: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRIS TVPD+FGKPKTVDEVLKI
Sbjct: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISRTVPDDFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL
Sbjct: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT
Sbjct: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGG D
Sbjct: 301 FEQKSDLRRALGGFD 315

BLAST of Carg01737 vs. ExPASy TrEMBL
Match: A0A5A7SYT8 (DnaJ-like protein subfamily B member 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00360 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.2e-161
Identity = 285/315 (90.48%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANE+DLKRSYK LAMKWHPDKNP NKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEDDLKRSYKRLAMKWHPDKNPCNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEAL SADFVPPP+SNPSFSY PRDADDIFAEFFGGAGS KS GF+ E L
Sbjct: 61  KKRQIYDLYGEEALKSADFVPPPNSNPSFSYVPRDADDIFAEFFGGAGSGKSRGFRGEGL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           F+NGKAEA K++NRKAPPIESKL+CSLEE+YKGSRRKMRIS TV DEFGKPKTVDEVLKI
Sbjct: 121 FKNGKAEAVKQTNRKAPPIESKLLCSLEELYKGSRRKMRISRTVADEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTK+TFPEKGNQEPGV+PADLIFIIDEKPHPVFERDGNDLV+NQK+SLLEAL
Sbjct: 181 DIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVNQKMSLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLN+TTLDGR+LT+VTDIVKPGYEVV QNEGMPISKEPNKKGNLRIKFDI+FPS LT
Sbjct: 241 TGKTLNITTLDGRDLTTVTDIVKPGYEVVFQNEGMPISKEPNKKGNLRIKFDIVFPSKLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 301 FEQKSDLRRALGGSD 315

BLAST of Carg01737 vs. ExPASy TrEMBL
Match: A0A1S3BFJ0 (dnaJ homolog subfamily B member 1 OS=Cucumis melo OX=3656 GN=LOC103489072 PE=4 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.2e-161
Identity = 285/315 (90.48%), Postives = 302/315 (95.87%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANE+DLKRSYK LAMKWHPDKNP NKKEAEAKFKQISEAYDVLSDA
Sbjct: 1   MGVDYYNVLKVNRNANEDDLKRSYKRLAMKWHPDKNPCNKKEAEAKFKQISEAYDVLSDA 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEAL SADFVPPP+SNPSFSY PRDADDIFAEFFGGAGS KS GF+ E L
Sbjct: 61  KKRQIYDLYGEEALKSADFVPPPNSNPSFSYVPRDADDIFAEFFGGAGSGKSRGFRGEGL 120

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           F+NGKAEA K++NRKAPPIESKL+CSLEE+YKGSRRKMRIS TV DEFGKPKTVDEVLKI
Sbjct: 121 FKNGKAEAVKQTNRKAPPIESKLLCSLEELYKGSRRKMRISRTVADEFGKPKTVDEVLKI 180

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTK+TFPEKGNQEPGV+PADLIFIIDEKPHPVFERDGNDLV+NQK+SLLEAL
Sbjct: 181 DIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVNQKMSLLEAL 240

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLN+TTLDGR+LT+VTDIVKPGYEVV QNEGMPISKEPNKKGNLRIKFDI+FPS LT
Sbjct: 241 TGKTLNITTLDGRDLTTVTDIVKPGYEVVFQNEGMPISKEPNKKGNLRIKFDIVFPSKLT 300

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 301 FEQKSDLRRALGGSD 315

BLAST of Carg01737 vs. ExPASy TrEMBL
Match: A0A0A0KSI9 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G604140 PE=4 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 3.6e-161
Identity = 286/315 (90.79%), Postives = 301/315 (95.56%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVL VNRNANE+DLKRSYK LAMKWHPDKNP NKKEAEAKFKQISEAYDVLSDA
Sbjct: 2   MGVDYYNVLMVNRNANEDDLKRSYKRLAMKWHPDKNPYNKKEAEAKFKQISEAYDVLSDA 61

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSNPSFSYFPRDADDIFAEFFGGAGSAKSGGFKRESL 120
           KKRQIYDLYGEEAL SADFVPPP+SNPSFSY PRDADDIFAEFFGGAGS KS GF+ E L
Sbjct: 62  KKRQIYDLYGEEALKSADFVPPPNSNPSFSYIPRDADDIFAEFFGGAGSGKSRGFRGEGL 121

Query: 121 FRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKI 180
           F+NGKAEA K++NRKAP IESKL+CSLEE+YKGSRRKMRIS TVPDEFGKPKTVDEVLKI
Sbjct: 122 FKNGKAEAVKQTNRKAPAIESKLLCSLEELYKGSRRKMRISRTVPDEFGKPKTVDEVLKI 181

Query: 181 DIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGNDLVINQKISLLEAL 240
           DIKPGWKKGTK+TFPEKGNQEPGV+PADLIFIIDEKPHPVFERDGNDLV+NQKISLLEAL
Sbjct: 182 DIKPGWKKGTKITFPEKGNQEPGVAPADLIFIIDEKPHPVFERDGNDLVVNQKISLLEAL 241

Query: 241 TGKTLNVTTLDGRNLTSVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLT 300
           TGKTLN+TTLDGR+L +VTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPS LT
Sbjct: 242 TGKTLNITTLDGRDLPTVTDIVKPGYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSKLT 301

Query: 301 FEQKSDLKRALGGSD 316
           FEQKSDL+RALGGSD
Sbjct: 302 FEQKSDLRRALGGSD 316

BLAST of Carg01737 vs. TAIR 10
Match: AT1G10350.1 (DNAJ heat shock family protein )

HSP 1 Score: 432.6 bits (1111), Expect = 2.8e-121
Identity = 223/350 (63.71%), Postives = 268/350 (76.57%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYYNVLKVNRNANE+DLK+SY+ +AMKWHPDKNP++KKEAEAKFKQISEAYDVLSD 
Sbjct: 1   MGVDYYNVLKVNRNANEDDLKKSYRRMAMKWHPDKNPTSKKEAEAKFKQISEAYDVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPD------------SNPSFSYFPRDADDIFAEFFGGAG 120
           ++RQIYD YGEE L S D     +            SN  F Y+PRDA+DIFAEFFG +G
Sbjct: 61  QRRQIYDQYGEEGLKSTDLPTAAETAAHQQQRSYSSSNSEFRYYPRDAEDIFAEFFGESG 120

Query: 121 SAKSGGFKRESLFRNG--------KAEAGKKSNRKAPP---------------IESKLMC 180
            A  GG    +    G         AEAG ++NRK PP               IESKL C
Sbjct: 121 DAFGGGSSGRTRGDGGDGGGRRFKSAEAGSQANRKTPPTNKKTTPPANRKAPAIESKLAC 180

Query: 181 SLEEIYKGSRRKMRISHTVPDEFGKPKTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVS 240
           +LEE+YKG+++KMRIS  VPD+FGKPKTV E+LKIDIKPGWKKGTK+TFPEKGNQEPGV+
Sbjct: 181 TLEELYKGAKKKMRISRVVPDDFGKPKTVQEILKIDIKPGWKKGTKITFPEKGNQEPGVT 240

Query: 241 PADLIFIIDEKPHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNLT-SVTDIVKP 300
           PADLIF++DEKPH VF+RDGNDL++ +K+SL++ALTG T++VTTLDGR+LT  V DIVKP
Sbjct: 241 PADLIFVVDEKPHSVFKRDGNDLILEKKVSLIDALTGLTISVTTLDGRSLTIPVLDIVKP 300

Query: 301 GYEVVIQNEGMPISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRALGGS 315
           G E+VI NEGMP +K+P K+G+LR+ F+I+FPS LT EQK+DLKR LGGS
Sbjct: 301 GQEIVIPNEGMP-TKDPLKRGDLRVTFEILFPSRLTSEQKNDLKRVLGGS 349

BLAST of Carg01737 vs. TAIR 10
Match: AT1G59725.1 (DNAJ heat shock family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.7e-115
Identity = 216/333 (64.86%), Postives = 257/333 (77.18%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSN-KKEAEAKFKQISEAYDVLSD 60
           MGVDYYNVL VN +A E+DLK+SY+ LAMKWHPDKNP++ K+EAEAKFKQISEAYDVLSD
Sbjct: 1   MGVDYYNVLNVNPSATEDDLKKSYRRLAMKWHPDKNPTSIKQEAEAKFKQISEAYDVLSD 60

Query: 61  AKKRQIYDLYGEEALNSADFVPPPDS-----------NPSFSYFPRDADDIFAEFFGGAG 120
             KRQIYD YGE+ L + +                  N  F Y+PRDA+DIFAEFFG + 
Sbjct: 61  PNKRQIYDQYGEDGLTATEATASSQQHNYSSGNNNNYNAGFRYYPRDAEDIFAEFFGASE 120

Query: 121 SAKSGGFKRESLFRNGKAEAGKKS------NRKAPPIESKLMCSLEEIYKGSRRKMRISH 180
               GG      F++  AEAG ++      NRKAP IESKL C+LEE+YKG RRKM+IS 
Sbjct: 121 KVFDGGVGGGGRFKS--AEAGSQTNRKTPVNRKAPAIESKLACTLEELYKGGRRKMKISR 180

Query: 181 TVPDEFGKPKTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFE 240
            VPD  GK K V+E+LKIDI PGWKKGTK+TFPEKGNQEPGV+PADLIF+IDEKPH V++
Sbjct: 181 VVPDGLGKSKPVEEILKIDITPGWKKGTKITFPEKGNQEPGVTPADLIFVIDEKPHSVYK 240

Query: 241 RDGNDLVINQKISLLEALTGKTLNVTTLDGRNLT-SVTDIVKPGYEVVIQNEGMPISKEP 300
           RDGNDL++++K+SLLEALTG TL++TTLDGRNLT  V DIVKPG E+VI +EGMPISKE 
Sbjct: 241 RDGNDLIVDKKVSLLEALTGITLSLTTLDGRNLTIPVLDIVKPGQEIVIPSEGMPISKEG 300

Query: 301 NKKGNLRIKFDIIFPSMLTFEQKSDLKRALGGS 315
           +K+G+LRI F+I FPS LT EQK+DLKR LGGS
Sbjct: 301 SKRGDLRINFEICFPSRLTSEQKTDLKRVLGGS 331

BLAST of Carg01737 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 372.1 bits (954), Expect = 4.5e-103
Identity = 195/339 (57.52%), Postives = 247/339 (72.86%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYY VL+V+R+A+++DLK++Y+ LAMKWHPDKNP+NKK+AEA FKQISEAY+VLSD 
Sbjct: 1   MGVDYYKVLQVDRSASDDDLKKAYRKLAMKWHPDKNPNNKKDAEAMFKQISEAYEVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPPDSN-----------PSFSYFPRDADDIFAEFFGGA-- 120
           +K+ +YD YGEE L     VPPPD+             SF + PR+ADDIFAEFFG +  
Sbjct: 61  QKKAVYDQYGEEGLKGN--VPPPDAGGATYFSTGDGPTSFRFNPRNADDIFAEFFGFSSP 120

Query: 121 -GSAKSGGFKRESLF---------RNGKAEAGKKSN---RKAPPIESKLMCSLEEIYKGS 180
            G  + G     S+F           G    G   +   RKA PIE+KL CSLE++YKG+
Sbjct: 121 FGGGRGGTRFSSSMFGDNMFASFGEGGGGGGGSMHHGGARKAAPIENKLPCSLEDLYKGT 180

Query: 181 RRKMRISHTVPDEFGKPKTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIID 240
            +KMRIS  + D  GK   V+E+L ID+KPGWKKGTK+TFPEKGN++PGV PADL+FIID
Sbjct: 181 TKKMRISREIADVSGKTMQVEEILTIDVKPGWKKGTKITFPEKGNEQPGVIPADLVFIID 240

Query: 241 EKPHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNLT-SVTDIVKPGYEVVIQNE 300
           EKPHPVF R+GNDL++ QKISL+EALTG T+N+TTLDGR LT  VT++V P YE V+  E
Sbjct: 241 EKPHPVFTREGNDLIVTQKISLVEALTGYTVNLTTLDGRRLTIPVTNVVHPEYEEVVPKE 300

Query: 301 GMPISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRALG 313
           GMP+ K+  K+GNLRIKF+I FP+ LT EQK+ +K+ LG
Sbjct: 301 GMPLQKDQTKRGNLRIKFNIKFPTRLTSEQKTGVKKLLG 337

BLAST of Carg01737 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 370.2 bits (949), Expect = 1.7e-102
Identity = 192/329 (58.36%), Postives = 239/329 (72.64%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVDYY VL+V+RNA ++DLK++Y+ LAMKWHPDKNP+NKK+AEAKFKQISEAYDVLSD 
Sbjct: 1   MGVDYYKVLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKDAEAKFKQISEAYDVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPP-------DSNPSFSYFPRDADDIFAEFFG-------G 120
           +KR IYD YGEE L S    PPP       D   SF +  R ADDIF+EFFG        
Sbjct: 61  QKRAIYDQYGEEGLTSQ--APPPGAGGGFSDGGASFRFNGRSADDIFSEFFGFTRPFGDS 120

Query: 121 AGSAKSGGFKRESLFRNGKAEAGKKSNRKAPPIESKLMCSLEEIYKGSRRKMRISHTVPD 180
            G+  S GF+    F      +     RKA PIE +L CSLE++YKG  +KM+IS  V D
Sbjct: 121 RGAGPSNGFR----FAEDVFSSNVVPPRKAAPIERQLPCSLEDLYKGVSKKMKISRDVLD 180

Query: 181 EFGKPKTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEKPHPVFERDGN 240
             G+P TV+E+L I+IKPGWKKGTK+TFPEKGN++ G+ P+DL+FI+DEKPH VF+RDGN
Sbjct: 181 SSGRPTTVEEILTIEIKPGWKKGTKITFPEKGNEQRGIIPSDLVFIVDEKPHAVFKRDGN 240

Query: 241 DLVINQKISLLEALTGKTLNVTTLDGRNLT-SVTDIVKPGYEVVIQNEGMPISKEPNKKG 300
           DLV+ QKI L+EALTG T  V+TLDGR++T  + +++ P YE V++ EGMPI K+P+KKG
Sbjct: 241 DLVMTQKIPLVEALTGYTAQVSTLDGRSVTVPINNVISPSYEEVVKGEGMPIPKDPSKKG 300

Query: 301 NLRIKFDIIFPSMLTFEQKSDLKRALGGS 315
           NLRIKF + FPS LT EQKS +KR    S
Sbjct: 301 NLRIKFTVKFPSRLTTEQKSGIKRMFSSS 323

BLAST of Carg01737 vs. TAIR 10
Match: AT5G01390.1 (DNAJ heat shock family protein )

HSP 1 Score: 367.5 bits (942), Expect = 1.1e-101
Identity = 198/336 (58.93%), Postives = 250/336 (74.40%), Query Frame = 0

Query: 1   MGVDYYNVLKVNRNANEEDLKRSYKSLAMKWHPDKNPSNKKEAEAKFKQISEAYDVLSDA 60
           MGVD+Y VL+V+R+AN+++LK++Y+ LAMKWHPDKNP+NKKEAEAKFKQISEAYDVLSD 
Sbjct: 1   MGVDFYKVLEVDRSANDDELKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDP 60

Query: 61  KKRQIYDLYGEEALNSADFVPPP----------DSNPSFSYFPRDADDIFAEFFG----- 120
           +KR IY+ YGEE LN A   PPP          D+  SF + PR ADDIF+EFFG     
Sbjct: 61  QKRAIYEQYGEEGLNQA---PPPGAGGGYPGGSDAGASFRFNPRSADDIFSEFFGFTRPS 120

Query: 121 -GAGS---AKSGGFK-RESLFRNGKA--EAGKKS--NRKAPPIESKLMCSLEEIYKGSRR 180
            G GS   A   GF+  + +F + +A    G+ S  +RK+ PIE +L CSLE++YKG  +
Sbjct: 121 FGTGSDSRAGPSGFRYGDDIFASFRAATTGGEASIPSRKSAPIERQLPCSLEDLYKGVSK 180

Query: 181 KMRISHTVPDEFGKPKTVDEVLKIDIKPGWKKGTKVTFPEKGNQEPGVSPADLIFIIDEK 240
           KM+IS  V D  G+P  V+E+L I+IKPGWKKGTK+TF EKGN+  GV P+DL+FI+DEK
Sbjct: 181 KMKISRDVLDSSGRPTPVEEILTIEIKPGWKKGTKITFLEKGNEHRGVIPSDLVFIVDEK 240

Query: 241 PHPVFERDGNDLVINQKISLLEALTGKTLNVTTLDGRNLT-SVTDIVKPGYEVVIQNEGM 300
           PHPVF+RDGNDLV+ QKISL++ALTG T  VTTLDGR LT  V +++ P YE V++ EGM
Sbjct: 241 PHPVFKRDGNDLVVMQKISLVDALTGYTAQVTTLDGRTLTVPVNNVISPSYEEVVKGEGM 300

Query: 301 PISKEPNKKGNLRIKFDIIFPSMLTFEQKSDLKRAL 312
           PI K+P++KGNLRI+F I FPS LT EQKS +KR L
Sbjct: 301 PIPKDPSRKGNLRIRFIIKFPSKLTTEQKSGIKRML 333

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7032180.13.7e-176100.00DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6601400.13.1e-17599.37DnaJ-like subfamily B member 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023537929.17.0e-17599.37dnaJ homolog subfamily B member 4-like [Cucurbita pepo subsp. pepo][more]
XP_022957391.12.0e-17499.05dnaJ homolog subfamily B member 4-like [Cucurbita moschata][more]
XP_022999795.17.7e-17498.73dnaJ homolog subfamily B member 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9D8322.0e-6341.30DnaJ homolog subfamily B member 4 OS=Mus musculus OX=10090 GN=Dnajb4 PE=1 SV=1[more]
P256856.4e-6240.52DnaJ homolog subfamily B member 1 OS=Homo sapiens OX=9606 GN=DNAJB1 PE=1 SV=4[more]
Q5R8J86.4e-6240.41DnaJ homolog subfamily B member 4 OS=Pongo abelii OX=9601 GN=DNAJB4 PE=2 SV=1[more]
Q3MI008.3e-6240.52DnaJ homolog subfamily B member 1 OS=Bos taurus OX=9913 GN=DNAJB1 PE=2 SV=3[more]
Q9UDY41.1e-6140.41DnaJ homolog subfamily B member 4 OS=Homo sapiens OX=9606 GN=DNAJB4 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GZ309.8e-17599.05dnaJ homolog subfamily B member 4-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1KGL13.7e-17498.73dnaJ homolog subfamily B member 4-like OS=Cucurbita maxima OX=3661 GN=LOC1114940... [more]
A0A5A7SYT81.2e-16190.48DnaJ-like protein subfamily B member 1 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3BFJ01.2e-16190.48dnaJ homolog subfamily B member 1 OS=Cucumis melo OX=3656 GN=LOC103489072 PE=4 S... [more]
A0A0A0KSI93.6e-16190.79J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G604140 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G10350.12.8e-12163.71DNAJ heat shock family protein [more]
AT1G59725.11.7e-11564.86DNAJ heat shock family protein [more]
AT2G20560.14.5e-10357.52DNAJ heat shock family protein [more]
AT3G08910.11.7e-10258.36DNAJ heat shock family protein [more]
AT5G01390.11.1e-10158.93DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 40..60
NoneNo IPR availableGENE3D2.60.260.20coord: 138..216
e-value: 3.4E-27
score: 96.3
NoneNo IPR availableGENE3D2.60.260.20coord: 218..297
e-value: 4.7E-25
score: 89.7
NoneNo IPR availablePANTHERPTHR24078:SF175DNAJ HEAT SHOCK FAMILY PROTEINcoord: 1..313
NoneNo IPR availablePANTHERPTHR24078DNAJ HOMOLOG SUBFAMILY C MEMBERcoord: 1..313
NoneNo IPR availableCDDcd10747DnaJ_Ccoord: 137..299
e-value: 6.74515E-48
score: 156.046
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 42..62
score: 57.34
coord: 62..81
score: 32.95
coord: 6..24
score: 39.64
coord: 24..39
score: 53.15
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 3..62
e-value: 3.8E-27
score: 106.2
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 4..67
e-value: 3.3E-25
score: 88.0
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 4..70
score: 22.898062
IPR001623DnaJ domainCDDcd06257DnaJcoord: 4..59
e-value: 3.48068E-21
score: 82.9785
IPR002939Chaperone DnaJ, C-terminalPFAMPF01556DnaJ_Ccoord: 139..296
e-value: 8.7E-44
score: 149.4
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 3..116
e-value: 7.6E-32
score: 112.1
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 3..107
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 47..66
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 138..219
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILY49493HSP40/DnaJ peptide-binding domaincoord: 220..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01737-RACarg01737-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0005829 cytosol
molecular_function GO:0051087 chaperone binding
molecular_function GO:0051082 unfolded protein binding