Carg01726 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01726
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAlpha-amylase
LocationCarg_Chr04: 9632427 .. 9636148 (+)
RNA-Seq ExpressionCarg01726
SyntenyCarg01726
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGGTAACATTGATGAGCTGAAGGATGCTGTGAAGACGTTCCATGATGTTGGGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACATTTTAAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGAACGTGCTGTAGTTTCAGATGATCCACATTTTCAGGTTTGATGTTTTATTAATTCGATCATTGATATGAATGATTATCAACTTTTCATATGCGATCTAAAATTAATTATCTTATACTGAGGATGAAGAATGAGAGAAAATTCTTACAATGCAAATACTTCATAACCCCATTTCTGTTATAAAAACCTTGACAGCACACATGTAAATGTCCCATTGATTATTCAATTTTCATGCCATGTGAAGGGTAGGGGCAACAAAAGTAGCGGAGACAACTTCCATGCTGCGCCTAACATTGATCATTCACAAGATTTTGTGAGAAATGATATTAAAGAATGGTTATGCTGGTTAAGGTTAATTCCCCAACCAATCCTTTTCTGGCTGAACTATTAATTTCTGTGGAACTTTTATTGGTTTATTTCAAAGTTTCTAGTCTTCTATGTGAAGACCTTATTGTATTGTGACATGATAACTCATAAGTTTGACATAGCGGTGAAAAGAGGCTTTGGACTGAACCAAGAGGTCCCTAGTTCAATGCCCTCTCATTGCATAGCTGCAATTTGAGACCCTTTTTGCACTGATAGGTGCCTGATATGATTAGATATAATTCCTCTCTTTGAACTTGTGAACAAAAAAAATTAAGGACATTTTCTAAAAAAATGTTAAATTAACCAAATAAGTGAAGAGTTTGAGTTATGTTGGTACATGGTGTGAGATATCACATCAGTTGGAGAGGGGAACGGAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAACCTTGAGAGAAAGCCTGGAAGGGAAAGCCCAAAGATGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGTATCAGAGCCAGACATCGAGCGGTGTGCCAGTGAGTATGTTGGTCCCCTAAGAGGGGTGGATTGTGAGATCTCACATCAATTGGAGAGGAGAACGAAGTATTCCTTATAAGGGTGTGGAAATCTCTCCCTAGTAGACGTGTTTTAAAACCTTGAGGAGAAGCCCAGAAGGTAAAGCTTAAAGAGGACAATATTTGCTAGCGGTGAGCTTGAGCTGTTACACATGAGCTCCTGGATCATGCTTGATTTTCTTATCAATCTTTTTAACGTTTGTTCCTTGAAATGAAACCATGCGGCTATCTTTAAATTATTAATTGGTGGGTTCATTCTGGTTATTTTAACGAAGTGCTTGATGTTCTTAACTTCTATGATTTGCTTATATACGTATGCACATAGGTTTCCTAATCAATTCAAATGTCACTTTTTCACTTTGTTTTTAAGGATTAGAGTAGCCATGTTTTATGCATATGTTTCTGGCTGTTGACATGCAGAAAGGAAATTGGGTACGATGGGTGGAGGCTTGATTTTGTCAGAGGATTCTGGGGTGGTTATGTGAAGGATTACTTGGATGCAAGTGAACCTTACTTTGCTGTTGGCGAATACTGGGATTCCCTGAGCTATACGTATGGTGTGATGGATTATAATCAAGATGCTCATAGACAGAGAATCATTGACTGGATAAATGCTACTAATGGAACTGCTGCAGCATTTGATGTTACTACCAAAGGGATCCTTCATTCTGTAAGCTTTCATTTAATTAACTGGTTCTCAATTATGTGGAAGAAAATTTCTGAGCTTATTCTCCTTTTCCTAATATGAACCTTTGAGTCATCAAAGCCTTTTTTACTCGGCCTAGTCTGGTCGCCCTACGCGACTGGCGTGGCCGAAAGGCGGAACTTTCATCGCAGTTTGGCGACCTAAGGGGCTGACACTTTTTTTTTTTTTGTTATCTAAAGGCGAAGACTCTGAAGTGAACAAACACTCGGCCTAGTGGGCGAACATGGCAGGCTTCAGCTTCATGCGCCTGCTGACACCTTTCGAAGCACTTTTGAGTGTGCGCTTTAGTTTTCGAATGTGTTGTTCAGCCTCACAAGTCTACAAGCCTTTCTTGTTCCATTGCTCGCCTTTTAGTAGGGGCCTTTAGTCTTTTGGTTAGAGTAGGGGTCCCAAGAGAGCCATAGTCGCAACTGTTGTCATAGTTAACAAGGTTGAAACTTTCAGGAAAAAACTTCGAATTGGGAGAGTGATCCTCCCAGTGAACTGACCGTATCCCAAACTGACACAGGTGAACAAGTAGAGTATACTAGGACACTTGAGAGAAACATGTCAAAGAAACTCGGCAAAATGACCCCGTAACTTCAGGAGAAGGAGTGCTCTCCCATCTTTTGATTAGGAAAGTGGCACATACCAGGGGGTAACGACGGTTTATTAAAAACACAGGATTAAACTCGTAAGAAAAAGAATTTTTATCACATTGTCATAACTTCTACTTTCTCCTTGTGTCTGTTTTAGATGTGTCTGATGGATTCAATTAGAAAAATCATATTGGTGTTCATTTTAGACAGTACTTCTGCAGCAATCCCTAAGATTCTTTGATTCTTACTCGTAGAACGTTATACACTTCTGAGATGCTTATTATATTTTTCCATGTTCTTTTTTCAGTAACCATTTGGTAAATCTCTGTTATAGGCAGTTGATAGATGTGAATATTGGCGTTTATCAGATCAGAAACGAAAGCCTCCTGGAGTCGTTGGATGGTGGCCTTCTCGTGCTGTTACCTTTATAGAGAATCACGACACTGGATCTACTCAGGTTTAAACATCTTTCCATAACAACCAGTTTATTGCATGAAATCAACTGGGTGAAACATTCATTACAGATACACTCTGATGTTTGTACTTTGGATGTTCTTCCATTTTAAAACTTCAAATTAATTTGGTGATGGTGTTTTCTGATGCTGTAGGGTCATTGGAGATTTCCCAGTGGAAAAGAAATGCAAGGGTATGCTTACATCCTGACTCATCCTGGGACACCATCACTATTCTTTGACCACATTTTCTCCCACTACAAATCTGAGATTGCAACACTTATTTGTCTTAGAAAACGGCAGAAACTTCACTGCCGGAGCGTAGTAAGTATGAATGATCTTTTTAAAAGATTGAAGAAAATTGCTATTGGCCTATGCCTTCACAGATGAAGAACAAGTATAAGTAGGCTTTAATCACTCTGTCCTGTAATTTTCAGGTTAAAATTGAGAAGGCAGAAAGGGATGTGTATGCAGCCACCATGGATGAAAAAGTTGGAGTGAAGATTGGACCAGGCTATTTTGAACCTCCTAGAGGATCCAGTGGATGGTCATTGGTTATGGAGGGAAAAGACTACAAGGTCTGGGAAGTATCCATCTAACAAATAACTATGTATCGTGAAAAGCTATATATATGGTAAAGTTGAACTATGTCTTCTCTGTTGTTGTACATCATTGAAGTCATCAGTATAAACCTTTATCTCTTATTTTTTCGTGTTCATCTAGTTCGAGTGTGCGTTATTGCGAGATCTTAATGGCAAAGTGCACCAGTTGATGGCAATGTATAAACTTATGGGCAGGTTAATATTATAGGAGACGTGGGTGATGCCATTGTATATATCTTAGACATGTCCATGAAATGGGGACGAATTGAAAATGTAAATATCTTAGAGATGTC

mRNA sequence

TATGGTAACATTGATGAGCTGAAGGATGCTGTGAAGACGTTCCATGATGTTGGGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACATTTTAAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGAACGTGCTGTAGTTTCAGATGATCCACATTTTCAGGGTAGGGGCAACAAAAGTAGCGGAGACAACTTCCATGCTGCGCCTAACATTGATCATTCACAAGATTTTGTGAGAAATGATATTAAAGAATGGTTATGCTGGTTAAGAAAGGAAATTGGGTACGATGGGTGGAGGCTTGATTTTGTCAGAGGATTCTGGGGTGGTTATGTGAAGGATTACTTGGATGCAAGTGAACCTTACTTTGCTGTTGGCGAATACTGGGATTCCCTGAGCTATACGTATGGTGTGATGGATTATAATCAAGATGCTCATAGACAGAGAATCATTGACTGGATAAATGCTACTAATGGAACTGCTGCAGCATTTGATGTTACTACCAAAGGGATCCTTCATTCTGCAGTTGATAGATGTGAATATTGGCGTTTATCAGATCAGAAACGAAAGCCTCCTGGAGTCGTTGGATGGTGGCCTTCTCGTGCTGTTACCTTTATAGAGAATCACGACACTGGATCTACTCAGGGTCATTGGAGATTTCCCAGTGGAAAAGAAATGCAAGGGTATGCTTACATCCTGACTCATCCTGGGACACCATCACTATTCTTTGACCACATTTTCTCCCACTACAAATCTGAGATTGCAACACTTATTTGTCTTAGAAAACGGCAGAAACTTCACTGCCGGAGCGTAGTTAAAATTGAGAAGGCAGAAAGGGATGTGTATGCAGCCACCATGGATGAAAAAGTTGGAGTGAAGATTGGACCAGGCTATTTTGAACCTCCTAGAGGATCCAGTGGATGGTCATTGGTTATGGAGGGAAAAGACTACAAGGTCTGGGAAGTATCCATCTAACAAATAACTATGTATCGTGAAAAGCTATATATATGGTAAAGTTGAACTATGTCTTCTCTGTTGTTGTACATCATTGAAGTCATCAGTATAAACCTTTATCTCTTATTTTTTCGTGTTCATCTAGTTCGAGTGTGCGTTATTGCGAGATCTTAATGGCAAAGTGCACCAGTTGATGGCAATGTATAAACTTATGGGCAGGTTAATATTATAGGAGACGTGGGTGATGCCATTGTATATATCTTAGACATGTCCATGAAATGGGGACGAATTGAAAATGTAAATATCTTAGAGATGTC

Coding sequence (CDS)

TATGGTAACATTGATGAGCTGAAGGATGCTGTGAAGACGTTCCATGATGTTGGGATAAAGGTTCTTGGTGATGTTGTCCTAAATCATCGTTGTGCACATTTTAAAAATCAAAATGGTATTTGGAATATATTTGGTGGTCGCTTAAATTGGGATGAACGTGCTGTAGTTTCAGATGATCCACATTTTCAGGGTAGGGGCAACAAAAGTAGCGGAGACAACTTCCATGCTGCGCCTAACATTGATCATTCACAAGATTTTGTGAGAAATGATATTAAAGAATGGTTATGCTGGTTAAGAAAGGAAATTGGGTACGATGGGTGGAGGCTTGATTTTGTCAGAGGATTCTGGGGTGGTTATGTGAAGGATTACTTGGATGCAAGTGAACCTTACTTTGCTGTTGGCGAATACTGGGATTCCCTGAGCTATACGTATGGTGTGATGGATTATAATCAAGATGCTCATAGACAGAGAATCATTGACTGGATAAATGCTACTAATGGAACTGCTGCAGCATTTGATGTTACTACCAAAGGGATCCTTCATTCTGCAGTTGATAGATGTGAATATTGGCGTTTATCAGATCAGAAACGAAAGCCTCCTGGAGTCGTTGGATGGTGGCCTTCTCGTGCTGTTACCTTTATAGAGAATCACGACACTGGATCTACTCAGGGTCATTGGAGATTTCCCAGTGGAAAAGAAATGCAAGGGTATGCTTACATCCTGACTCATCCTGGGACACCATCACTATTCTTTGACCACATTTTCTCCCACTACAAATCTGAGATTGCAACACTTATTTGTCTTAGAAAACGGCAGAAACTTCACTGCCGGAGCGTAGTTAAAATTGAGAAGGCAGAAAGGGATGTGTATGCAGCCACCATGGATGAAAAAGTTGGAGTGAAGATTGGACCAGGCTATTTTGAACCTCCTAGAGGATCCAGTGGATGGTCATTGGTTATGGAGGGAAAAGACTACAAGGTCTGGGAAGTATCCATCTAA

Protein sequence

YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVSI
Homology
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BLAST of Carg01726 vs. NCBI nr
Match: KAG7032191.1 (Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 724.5 bits (1869), Expect = 4.0e-205
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP
Sbjct: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL
Sbjct: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI
Sbjct: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV
Sbjct: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVSI 333
           KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVSI
Sbjct: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVSI 332

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BLAST of Carg01726 vs. NCBI nr
Match: KAG6601411.1 (Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 723.0 bits (1865), Expect = 1.2e-204
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0 

Query: 1    YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
            YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP
Sbjct: 1032 YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 1091

Query: 61   HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
            HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 1092 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 1151

Query: 121  KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
            KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL
Sbjct: 1152 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 1211

Query: 181  HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
            HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI
Sbjct: 1212 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 1271

Query: 241  LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
            LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV
Sbjct: 1272 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 1331

Query: 301  KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
            KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS
Sbjct: 1332 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 1362

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BLAST of Carg01726 vs. NCBI nr
Match: XP_023537683.1 (alpha-amylase 3, chloroplastic-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 715.7 bits (1846), Expect = 1.9e-202
Identity = 328/331 (99.09%), Postives = 329/331 (99.40%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHF+NQNG WNIFGGRLNWDERAVVSDDP
Sbjct: 569 YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFRNQNGSWNIFGGRLNWDERAVVSDDP 628

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 629 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 688

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL
Sbjct: 689 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 748

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI
Sbjct: 749 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 808

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPSLFFDHIFSHYKSEIATLI LRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV
Sbjct: 809 LTHPGTPSLFFDHIFSHYKSEIATLISLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 868

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS
Sbjct: 869 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 899

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BLAST of Carg01726 vs. NCBI nr
Match: XP_022998113.1 (alpha-amylase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 714.9 bits (1844), Expect = 3.2e-202
Identity = 327/331 (98.79%), Postives = 329/331 (99.40%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHF+NQNGIWNIFGGRLNWDERAVVSDDP
Sbjct: 462 YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFRNQNGIWNIFGGRLNWDERAVVSDDP 521

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 522 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 581

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYGVMD+NQDAHRQRIIDWINATNGTA AFDVTTKGIL
Sbjct: 582 KDYLDASEPYFAVGEYWDSLSYTYGVMDHNQDAHRQRIIDWINATNGTAGAFDVTTKGIL 641

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI
Sbjct: 642 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 701

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV
Sbjct: 702 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 761

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPG FEPPRGSSGWSLVMEGKDYKVWEVS
Sbjct: 762 KIGPGNFEPPRGSSGWSLVMEGKDYKVWEVS 792

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BLAST of Carg01726 vs. NCBI nr
Match: XP_022957043.1 (alpha-amylase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 707.6 bits (1825), Expect = 5.1e-200
Identity = 328/350 (93.71%), Postives = 330/350 (94.29%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHF+NQNGIWNIFGGRLNWDERAVVSDDP
Sbjct: 462 YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFRNQNGIWNIFGGRLNWDERAVVSDDP 521

Query: 61  HFQ-------------------GRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKE 120
           HFQ                   GRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKE
Sbjct: 522 HFQSDRTHVNVPLIIQFSCHVKGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKE 581

Query: 121 IGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDW 180
           IGYDGWRLDFVRGFWGGYVKDYLDASEPYF VGEYWDSLSYTYGVMDYNQDAHRQRIIDW
Sbjct: 582 IGYDGWRLDFVRGFWGGYVKDYLDASEPYFTVGEYWDSLSYTYGVMDYNQDAHRQRIIDW 641

Query: 181 INATNGTAAAFDVTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGS 240
           INATNGTAAAFDVTTKGILHSAVDRCEYWRLSD+KRKPPGVVGWWPSRAVTFIENHDTGS
Sbjct: 642 INATNGTAAAFDVTTKGILHSAVDRCEYWRLSDEKRKPPGVVGWWPSRAVTFIENHDTGS 701

Query: 241 TQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVK 300
           TQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVK
Sbjct: 702 TQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVK 761

Query: 301 IEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           IEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS
Sbjct: 762 IEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 811

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BLAST of Carg01726 vs. ExPASy Swiss-Prot
Match: Q94A41 (Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 2.1e-180
Identity = 277/331 (83.69%), Postives = 303/331 (91.54%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YG IDELKD VK FH VGIKVLGD VLNHRCAHFKNQNG+WN+FGGRLNWD+RAVV+DDP
Sbjct: 557 YGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDP 616

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYV
Sbjct: 617 HFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYV 676

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDY+DAS+PYFAVGEYWDSLSYTYG MDYNQDAHRQRI+DWINAT+G A AFDVTTKGIL
Sbjct: 677 KDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGIL 736

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           H+A+ +CEYWRLSD K KPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYI
Sbjct: 737 HTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYI 796

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTP++FFDHIFS Y SEIA L+ LR RQKLHCRS V I+K+ERDVYAA +DEKV +
Sbjct: 797 LTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAM 856

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPG++EPP GS  WS+ +EG+DYKVWE S
Sbjct: 857 KIGPGHYEPPNGSQNWSVAVEGRDYKVWETS 887

[Close]
BLAST of Carg01726 vs. ExPASy Swiss-Prot
Match: Q8LFG1 (Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 3.5e-95
Identity = 166/332 (50.00%), Postives = 221/332 (66.57%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGG-RLNWDERAVVSDD 60
           YG+   LK  ++      ++ + D+V+NHR    +   G++N + G  L WDE AV S  
Sbjct: 86  YGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCT 145

Query: 61  PHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGY 120
               G GN+S+GDNF+  PN+DH+Q FVR DI  WL WLR  +G+  +R DF RG+   Y
Sbjct: 146 ---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANY 205

Query: 121 VKDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGI 180
           VK+Y+ A++P F+VGE WDS +Y    +DYNQD+HRQRII WI+AT   +AAFD TTKGI
Sbjct: 206 VKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGI 265

Query: 181 LHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAY 240
           L  AV + +YWRL D + KPPGV+GWWPSRAVTF++NHDTGSTQ HW FPS   M+GYAY
Sbjct: 266 LQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAY 325

Query: 241 ILTHPGTPSLFFDHIF---SHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDE 300
           ILTHPG P +F+DH +   S    +I  LI +R+RQ +H RS V++ KAE ++YAA + E
Sbjct: 326 ILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGE 385

Query: 301 KVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVW 329
           K+ +K+G G + P      W+L   G  Y VW
Sbjct: 386 KICMKLGDGSWCP--SGRDWTLATSGHRYAVW 411

[Close]
BLAST of Carg01726 vs. ExPASy Swiss-Prot
Match: P27937 (Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.6 PE=2 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.4e-89
Identity = 158/339 (46.61%), Postives = 216/339 (63.72%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGG-----RLNWDERAV 60
           YG   EL+  +  FH  GIK + D+V+NHRCA +K+  GI+ IF G     RL+W    +
Sbjct: 90  YGTGAELRSLIAAFHSKGIKCVADIVINHRCADYKDSRGIYCIFEGGTPDSRLDWGPDMI 149

Query: 61  VSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRG 120
            SDD  +  GRG++ +G +F AAP+IDH    V+ ++ +WL WL+ ++G+DGWRLDF +G
Sbjct: 150 CSDDTQYSNGRGHRDTGADFGAAPDIDHLNTRVQTELSDWLNWLKSDVGFDGWRLDFAKG 209

Query: 121 FWGGYVKDYLDASEPYFAVGEYWDSLSYT-YGVMDYNQDAHRQRIIDWINATNGTAAAFD 180
           +     K Y+D ++P F V E W ++ Y   G   +NQD  RQ +++W  A  G A+AFD
Sbjct: 210 YSAAVAKTYVDNTDPSFVVAEIWSNMRYDGNGEPSWNQDGDRQELVNWAQAVGGPASAFD 269

Query: 181 VTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKE 240
            TTKG L +AV + E WR+ D   K PG++GW P +AVTFI+NHDTGSTQ  W FPS K 
Sbjct: 270 FTTKGELQAAV-QGELWRMKDGNGKAPGMIGWLPEKAVTFIDNHDTGSTQNSWPFPSDKV 329

Query: 241 MQGYAYILTHPGTPSLFFDHIFS-HYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAA 300
           MQGYAYILTHPG P +F+DH+F  + K EI+TL  +R R ++H  S +KI  AE DVY A
Sbjct: 330 MQGYAYILTHPGVPCIFYDHVFDWNLKQEISTLAAVRSRNEIHPGSKLKILAAEGDVYVA 389

Query: 301 TMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
            +D+KV  KIG  Y       S + +V  G +Y +WE S
Sbjct: 390 MIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCIWEKS 427

[Close]
BLAST of Carg01726 vs. ExPASy Swiss-Prot
Match: P00693 (Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 8.4e-89
Identity = 159/340 (46.76%), Postives = 216/340 (63.53%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIF-----GGRLNWDERAV 60
           YGN  ELK  +   H  G++ + D+V+NHRCA +K+  GI+ IF      GRL+W    +
Sbjct: 89  YGNAAELKSLIGALHGKGVQAIADIVINHRCADYKDSRGIYCIFEGGTSDGRLDWGPHMI 148

Query: 61  VSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRG 120
             DD  +  G  N  +G +F AAP+IDH  D V+ ++KEWL WL+ ++G+D WRLDF RG
Sbjct: 149 CRDDTKYSDGTANLDTGADFAAAPDIDHLNDRVQRELKEWLLWLKSDLGFDAWRLDFARG 208

Query: 121 FWGGYVKDYLDASEPYFAVGEYWDSLSY-TYGVMDYNQDAHRQRIIDWINATNGTAAA-- 180
           +     K Y+D + P  AV E WD+++    G  +Y+QDAHRQ +++W++   G A+A  
Sbjct: 209 YSPEMAKVYIDGTSPSLAVAEVWDNMATGGDGKPNYDQDAHRQNLVNWVDKVGGAASAGM 268

Query: 181 -FDVTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPS 240
            FD TTKGIL++AV+  E WRL D + K PGV+GWWP++A TF++NHDTGSTQ  W FPS
Sbjct: 269 VFDFTTKGILNAAVEG-ELWRLIDPQGKAPGVMGWWPAKAATFVDNHDTGSTQAMWPFPS 328

Query: 241 GKEMQGYAYILTHPGTPSLFFDHIFS-HYKSEIATLICLRKRQKLHCRSVVKIEKAERDV 300
            K MQGYAYILTHPG P +F+DH F+  +K +IA L+ +RKR  +   S +KI   E D 
Sbjct: 329 DKVMQGYAYILTHPGIPCIFYDHFFNWGFKDQIAALVAIRKRNGITATSALKILMHEGDA 388

Query: 301 YAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWE 330
           Y A +D KV VKIG  Y       +G+     G DY VWE
Sbjct: 389 YVAEIDGKVVVKIGSRYDVGAVIPAGFVTSAHGNDYAVWE 427

[Close]
BLAST of Carg01726 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 8.4e-89
Identity = 159/337 (47.18%), Postives = 213/337 (63.20%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIF-----GGRLNWDERAV 60
           YG   ELK  ++ FHD  ++ L D+V+NHRCA +K+  G++ +F      GRL+W    +
Sbjct: 89  YGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVYCVFEGGTPDGRLDWGPDMI 148

Query: 61  VSDDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRG 120
            SDD  +  GRG++ +G  F AAP+IDH    V+ ++ +WL WLR ++G+DGWRLDF +G
Sbjct: 149 CSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWLNWLRTDLGFDGWRLDFAKG 208

Query: 121 FWGGYVKDYLDASEPYFAVGEYWDSLSYT-YGVMDYNQDAHRQRIIDWINATNGTAAAFD 180
           +     + Y+D + P F VGE W SL Y   G    NQDA RQ +++W+      A AFD
Sbjct: 209 YSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDADRQELVNWVEGVGKPATAFD 268

Query: 181 VTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKE 240
            TTKGIL +AV + E WRL D   K PG++GW P +AVTF++NHDTGSTQ  W FPS K 
Sbjct: 269 FTTKGILQAAV-QGELWRLHDGNGKAPGLMGWMPDQAVTFVDNHDTGSTQSLWPFPSDKV 328

Query: 241 MQGYAYILTHPGTPSLFFDHIFS-HYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAA 300
           MQGYAYILTHPG P +F+DH+F  + + EIATL  +R R  +H  S + I KAE D+Y A
Sbjct: 329 MQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGIHAESTLDILKAEGDIYVA 388

Query: 301 TMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWE 330
            +D KV  K+GP Y       S + +V  G DY VWE
Sbjct: 389 MIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWE 424

[Close]
BLAST of Carg01726 vs. ExPASy TrEMBL
Match: A0A6J1KFW6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111492830 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.5e-202
Identity = 327/331 (98.79%), Postives = 329/331 (99.40%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHF+NQNGIWNIFGGRLNWDERAVVSDDP
Sbjct: 462 YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFRNQNGIWNIFGGRLNWDERAVVSDDP 521

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 522 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 581

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYGVMD+NQDAHRQRIIDWINATNGTA AFDVTTKGIL
Sbjct: 582 KDYLDASEPYFAVGEYWDSLSYTYGVMDHNQDAHRQRIIDWINATNGTAGAFDVTTKGIL 641

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI
Sbjct: 642 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 701

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV
Sbjct: 702 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 761

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPG FEPPRGSSGWSLVMEGKDYKVWEVS
Sbjct: 762 KIGPGNFEPPRGSSGWSLVMEGKDYKVWEVS 792

[Close]
BLAST of Carg01726 vs. ExPASy TrEMBL
Match: A0A6J1GZ33 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111458536 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 2.5e-200
Identity = 328/350 (93.71%), Postives = 330/350 (94.29%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHF+NQNGIWNIFGGRLNWDERAVVSDDP
Sbjct: 462 YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFRNQNGIWNIFGGRLNWDERAVVSDDP 521

Query: 61  HFQ-------------------GRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKE 120
           HFQ                   GRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKE
Sbjct: 522 HFQSDRTHVNVPLIIQFSCHVKGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKE 581

Query: 121 IGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDW 180
           IGYDGWRLDFVRGFWGGYVKDYLDASEPYF VGEYWDSLSYTYGVMDYNQDAHRQRIIDW
Sbjct: 582 IGYDGWRLDFVRGFWGGYVKDYLDASEPYFTVGEYWDSLSYTYGVMDYNQDAHRQRIIDW 641

Query: 181 INATNGTAAAFDVTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGS 240
           INATNGTAAAFDVTTKGILHSAVDRCEYWRLSD+KRKPPGVVGWWPSRAVTFIENHDTGS
Sbjct: 642 INATNGTAAAFDVTTKGILHSAVDRCEYWRLSDEKRKPPGVVGWWPSRAVTFIENHDTGS 701

Query: 241 TQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVK 300
           TQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVK
Sbjct: 702 TQGHWRFPSGKEMQGYAYILTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVK 761

Query: 301 IEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           IEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS
Sbjct: 762 IEKAERDVYAATMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 811

[Close]
BLAST of Carg01726 vs. ExPASy TrEMBL
Match: A0A5A7SY64 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G003970 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.3e-190
Identity = 305/331 (92.15%), Postives = 317/331 (95.77%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKD VKTFHDVGIKVLGD VLNHRCAHFKNQNGIWNIFGGRLNWD+RAVVSDDP
Sbjct: 570 YGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDP 629

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 630 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 689

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYG MD+NQDAHRQRI+DWINATNGTA AFDVTTKGIL
Sbjct: 690 KDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGIL 749

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSA+DRCEYWRLSD+K KPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAY+
Sbjct: 750 HSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYL 809

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPS+F+DHIFSHYKSEIA LI LRKR K++CRSVVKI KAERDVYAAT+DE V V
Sbjct: 810 LTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDETVAV 869

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPG FEPP GSSGWSLV+EGKDYKVWEVS
Sbjct: 870 KIGPGNFEPPSGSSGWSLVIEGKDYKVWEVS 900

[Close]
BLAST of Carg01726 vs. ExPASy TrEMBL
Match: A0A1S3BES1 (1,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.3e-190
Identity = 305/331 (92.15%), Postives = 317/331 (95.77%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKD VKTFHDVGIKVLGD VLNHRCAHFKNQNGIWNIFGGRLNWD+RAVVSDDP
Sbjct: 570 YGNIDELKDVVKTFHDVGIKVLGDAVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDP 629

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 630 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 689

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYG MD+NQDAHRQRI+DWINATNGTA AFDVTTKGIL
Sbjct: 690 KDYLDASEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIVDWINATNGTAGAFDVTTKGIL 749

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSA+DRCEYWRLSD+K KPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAY+
Sbjct: 750 HSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYL 809

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPS+F+DHIFSHYKSEIA LI LRKR K++CRSVVKI KAERDVYAAT+DE V V
Sbjct: 810 LTHPGTPSVFYDHIFSHYKSEIAALISLRKRNKVNCRSVVKIVKAERDVYAATIDETVAV 869

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPG FEPP GSSGWSLV+EGKDYKVWEVS
Sbjct: 870 KIGPGNFEPPSGSSGWSLVIEGKDYKVWEVS 900

[Close]
BLAST of Carg01726 vs. ExPASy TrEMBL
Match: A0A6J1DCP4 (1,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC111019134 PE=3 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 4.3e-189
Identity = 303/331 (91.54%), Postives = 317/331 (95.77%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YGNIDELKD VKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWD+RAVVSDDP
Sbjct: 570 YGNIDELKDVVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDDRAVVSDDP 629

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWL WLRKEIGYDGWRLDFVRGFWGGYV
Sbjct: 630 HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLRWLRKEIGYDGWRLDFVRGFWGGYV 689

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDYLDASEPYFAVGEYWDSLSYTYG MD++QDAHRQRI+DWINATNGTA AFDVTTKGIL
Sbjct: 690 KDYLDASEPYFAVGEYWDSLSYTYGEMDHDQDAHRQRIVDWINATNGTAGAFDVTTKGIL 749

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           HSA+DRCEYWRLSDQK KPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAY 
Sbjct: 750 HSALDRCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGKEMQGYAYT 809

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTPS+FFDHIFSHYKS+IA LI LRKR K++CRSVVKIEKAERDVYAAT+DEKV +
Sbjct: 810 LTHPGTPSVFFDHIFSHYKSDIAALISLRKRNKVNCRSVVKIEKAERDVYAATIDEKVAM 869

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPGY+EPP  S+GWSLV+EG DYKVWEVS
Sbjct: 870 KIGPGYYEPPSRSNGWSLVIEGNDYKVWEVS 900

[Close]
BLAST of Carg01726 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-181
Identity = 277/331 (83.69%), Postives = 303/331 (91.54%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGGRLNWDERAVVSDDP 60
           YG IDELKD VK FH VGIKVLGD VLNHRCAHFKNQNG+WN+FGGRLNWD+RAVV+DDP
Sbjct: 557 YGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGGRLNWDDRAVVADDP 616

Query: 61  HFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGYV 120
           HFQGRGNKSSGDNFHAAPNIDHSQDFVR DIKEWLCW+ +E+GYDGWRLDFVRGFWGGYV
Sbjct: 617 HFQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRLDFVRGFWGGYV 676

Query: 121 KDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGIL 180
           KDY+DAS+PYFAVGEYWDSLSYTYG MDYNQDAHRQRI+DWINAT+G A AFDVTTKGIL
Sbjct: 677 KDYMDASKPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWINATSGAAGAFDVTTKGIL 736

Query: 181 HSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYI 240
           H+A+ +CEYWRLSD K KPPGVVGWWPSRAVTFIENHDTGSTQGHWRFP GKEMQGYAYI
Sbjct: 737 HTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPEGKEMQGYAYI 796

Query: 241 LTHPGTPSLFFDHIFSHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDEKVGV 300
           LTHPGTP++FFDHIFS Y SEIA L+ LR RQKLHCRS V I+K+ERDVYAA +DEKV +
Sbjct: 797 LTHPGTPAVFFDHIFSDYHSEIAALLSLRNRQKLHCRSEVNIDKSERDVYAAIIDEKVAM 856

Query: 301 KIGPGYFEPPRGSSGWSLVMEGKDYKVWEVS 332
           KIGPG++EPP GS  WS+ +EG+DYKVWE S
Sbjct: 857 KIGPGHYEPPNGSQNWSVAVEGRDYKVWETS 887

[Close]
BLAST of Carg01726 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 349.7 bits (896), Expect = 2.5e-96
Identity = 166/332 (50.00%), Postives = 221/332 (66.57%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGG-RLNWDERAVVSDD 60
           YG+   LK  ++      ++ + D+V+NHR    +   G++N + G  L WDE AV S  
Sbjct: 86  YGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDGISLPWDEHAVTSCT 145

Query: 61  PHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGFWGGY 120
               G GN+S+GDNF+  PN+DH+Q FVR DI  WL WLR  +G+  +R DF RG+   Y
Sbjct: 146 ---GGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFARGYSANY 205

Query: 121 VKDYLDASEPYFAVGEYWDSLSYTYGVMDYNQDAHRQRIIDWINATNGTAAAFDVTTKGI 180
           VK+Y+ A++P F+VGE WDS +Y    +DYNQD+HRQRII WI+AT   +AAFD TTKGI
Sbjct: 206 VKEYIGAAKPLFSVGECWDSCNYNGHGLDYNQDSHRQRIISWIDATGQISAAFDFTTKGI 265

Query: 181 LHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAY 240
           L  AV + +YWRL D + KPPGV+GWWPSRAVTF++NHDTGSTQ HW FPS   M+GYAY
Sbjct: 266 LQEAV-KGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHVMEGYAY 325

Query: 241 ILTHPGTPSLFFDHIF---SHYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAATMDE 300
           ILTHPG P +F+DH +   S    +I  LI +R+RQ +H RS V++ KAE ++YAA + E
Sbjct: 326 ILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLYAAIVGE 385

Query: 301 KVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVW 329
           K+ +K+G G + P      W+L   G  Y VW
Sbjct: 386 KICMKLGDGSWCP--SGRDWTLATSGHRYAVW 411

[Close]
BLAST of Carg01726 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 287.7 bits (735), Expect = 1.2e-77
Identity = 150/337 (44.51%), Postives = 199/337 (59.05%), Query Frame = 0 

Query: 1   YGNIDELKDAVKTFHDVGIKVLGDVVLNHRCAHFKNQNGIWNIFGG-----RLNWDERAV 60
           YG+  ELK  +K  +  GIK L D+V+NHR A  K+    +  F G     RL+WD   V
Sbjct: 89  YGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDWDPSFV 148

Query: 61  VSDDPHFQGRGNKSSGDNFHAAPNIDHSQDFVRNDIKEWLCWLRKEIGYDGWRLDFVRGF 120
             +DP F G GN  +G +F  AP+IDH    V+ ++ EW+ WL+ EIG+ GWR D+VRG+
Sbjct: 149 CRNDPKFPGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRFDYVRGY 208

Query: 121 WGGYVKDYLDASEPYFAVGEYWDSLSY-TYGVMDYNQDAHRQRIIDWI-NATNGTAAAFD 180
                K Y+  + P FAVGE WD + Y   G +DY+Q+ HR  +  WI  A  G   AFD
Sbjct: 209 ASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGGVLTAFD 268

Query: 181 VTTKGILHSAVDRCEYWRLSDQKRKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKE 240
            TTKGIL SAV + E WRL D + KPPG++G  P  AVTFI+NHDT  T   W FPS K 
Sbjct: 269 FTTKGILQSAV-KGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHDTFRT---WVFPSDKV 328

Query: 241 MQGYAYILTHPGTPSLFFDHIFS-HYKSEIATLICLRKRQKLHCRSVVKIEKAERDVYAA 300
           + GY YILTHPGTP +F++H      K  I+ L+ +R +  +   S V I+ AE D+Y A
Sbjct: 329 LLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEADLYLA 388

Query: 301 TMDEKVGVKIGPGYFEPPRGSSGWSLVMEGKDYKVWE 330
            +D+KV +KIGP         S ++L   G D+ VWE
Sbjct: 389 MIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWE 421

The following BLAST results are available for this feature:
BLAST of Carg01726 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7032191.14.0e-205100.00Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6601411.11.2e-204100.00Alpha-amylase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023537683.11.9e-20299.09alpha-amylase 3, chloroplastic-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022998113.13.2e-20298.79alpha-amylase 3, chloroplastic-like [Cucurbita maxima][more]
XP_022957043.15.1e-20093.71alpha-amylase 3, chloroplastic-like [Cucurbita moschata][more]
BLAST of Carg01726 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q94A412.1e-18083.69Alpha-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AMY3 PE=1 SV=1[more]
Q8LFG13.5e-9550.00Probable alpha-amylase 2 OS=Arabidopsis thaliana OX=3702 GN=AMY2 PE=2 SV=1[more]
P279376.4e-8946.61Alpha-amylase isozyme 3B OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.6 PE=2... [more]
P006938.4e-8946.76Alpha-amylase type A isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.1 PE=1 SV=1[more]
P279348.4e-8947.18Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2... [more]
BLAST of Carg01726 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1KFW61.5e-20298.79Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111492830 PE=3 SV=1[more]
A0A6J1GZ332.5e-20093.71Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111458536 PE=3 SV=1[more]
A0A5A7SY641.3e-19092.151,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A1S3BES11.3e-19092.151,4-alpha-D-glucan glucanohydrolase OS=Cucumis melo OX=3656 GN=LOC103489088 PE=4... [more]
A0A6J1DCP44.3e-18991.541,4-alpha-D-glucan glucanohydrolase OS=Momordica charantia OX=3673 GN=LOC1110191... [more]
BLAST of Carg01726 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G69830.11.5e-18183.69alpha-amylase-like 3 [more]
AT1G76130.12.5e-9650.00alpha-amylase-like 2 [more]
AT4G25000.11.2e-7744.51alpha-amylase-like [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006046Alpha amylasePRINTSPR00110ALPHAAMYLASEcoord: 104..115
score: 32.03
coord: 18..29
score: 48.94
coord: 208..220
score: 46.76
IPR012850Alpha-amylase, C-terminal beta-sheetSMARTSM00810alpha-amyl_c2coord: 270..330
e-value: 3.6E-25
score: 99.6
IPR012850Alpha-amylase, C-terminal beta-sheetPFAMPF07821Alpha-amyl_C2coord: 271..329
e-value: 2.3E-20
score: 72.6
IPR006047Glycosyl hydrolase, family 13, catalytic domainSMARTSM00642aamycoord: 1..269
e-value: 1.5E-7
score: -1.4
IPR006047Glycosyl hydrolase, family 13, catalytic domainPFAMPF00128Alpha-amylasecoord: 1..221
e-value: 1.9E-8
score: 34.3
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..273
e-value: 5.5E-99
score: 333.9
NoneNo IPR availablePANTHERPTHR43447:SF33ALPHA-AMYLASE 3, CHLOROPLASTICcoord: 1..331
NoneNo IPR availablePANTHERPTHR43447ALPHA-AMYLASEcoord: 1..331
NoneNo IPR availableCDDcd11314AmyAc_arch_bac_plant_AmyAcoord: 1..280
e-value: 5.08819E-131
score: 373.865
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 274..329
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 274..330
e-value: 1.6E-18
score: 68.5
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 1..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01726-RACarg01726-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0004556 alpha-amylase activity
molecular_function GO:0103025 alpha-amylase activity (releasing maltohexaose)
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0043169 cation binding