Carg01191 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01191
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ganglioside-induced differentiation-associated protein 2-like
Location	Carg_Chr06: 756565 .. 758408 (+)
RNA-Seq Expression	Carg01191
Synteny	Carg01191

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTAATTTTATGAAAAAGGGATTTAACCGATATTAAAAAGCAGAGGTGTCATGTGGAGAGGGAAGGAGGGTAACAGAATATTCGTCTGTGACAAAAATCTTTGACCCACGTCGGCGCCATTTTATCGGCCACCATGATCTTCATTTCTTTCCACCTTTGTTAAAAATTCGATTCAATTCAATTCAATTATTCATTCTTCGTTCTTAATTTATTTTCCAAATCTCGTTCGACCAATTCCATCTCCACCAAATCCGTTCAATCCATCCTTATCCATATCCGTAGTTTGCCCTAACTATGATCTGCACGCTACTCCCTTTTTCCTTCTGATTCTAATCGGAGTTCTTCAACTATGTCCGCTCAGGTTCAGCCTCCTCCGCCGGAGCAAAACCAACTCGAAATGTTCGATGTCTTCAAGATCAAAGGCAGGGACAAACAGTGCCGCCGGATTCTCCGTATCGTCGGAAAATTCTTTCCAGGTTTTCGATTTCTCTCTCATTCTTCTTTTCGTCGATGAAAATTGAATTTTCGTCGTCTTTTTTACTACTGAGTTTGAATGTCCTCTCAACGAATATCTATATGTTTGCGTTACTGTGCTCTGTTTCTGTTTTCTTGTTCATGAAATTGAATTTATGTCATTGTCTTGAGCGCGAAGTGTGAGTTTGAATGCGCTCAAGAAGCATCTGTTTATTTGACCTAATCTGCTTTGCTTCGATTTTCCTGCTCGTTTTCATCGATGAGATTGAATTTCTGTTGTCTCTTTTGATCATCAGCGCGCACTGTGAGTTTGAATTTGTTCAAGAAGCAACTACATATTGTCTTACTCTGCTCTGATTTTGTTTTCTTGCTCTGTTCTGATTCGAGTTTCGTTCTATTTTCATCGATGAAATTGAAAATGAAGTATCTGCATAATTTGAACTACTAATGCTCCGATTCTAATTTCTTGCTGCTTTAATCGATGAGATTGAATACTGATCATCTTTTCTGATAATCAGCGCGGACTGTGAGTTTGGATGTGGTGAAGAATCATCTGGAGGAGAAGATCTTCCCTAGGCTTAAGAACAAGCGTTTCACGATCCTGTACTTTCACACCAGCGTTCAAAGGAGCCAAAATTTCCCCGGAATCGCCGCTCTCCGATCGATCTATGACGCAATTCCGGCCGCCGTCAAGGCTAATCTGGAAGCCGTGTATTTCGTTCACCCAGACCTCCAAGCCAGGCTCTTCCTCGCCACTCTCGGCCGCATTTTCTTCAGCAGCGAGTACAATTTTCTAACCTTTTTCCTTTAATCAATTTCAATTTTCCCCCAATTTTCTTGACTATGGTAATCAATTTCACACGTACGAAACGTCAAATCCACATTTTAATTTTTTTTATTAAAAAAAAACGTAAAATATATTTTTTTTGTAATTTTAAATAAGTTGATAATGTATGGGCTGACCCTTTTCTTTTAGAAAAACACATCTGAGGGAAAAAAAAAAAAAGTATTTTTGGACTCTTCACAGAAATAGTTGTATGTAAAATTATATTTATGCCCTTACTCGCTAAATAAAATAAAAAAGAGTAGAAACAATTGAATTTCTTTTTTTTTTTTGAACTTTTTATTAATGGGGGTAATATTTGAATGTGGTAGGGTGTATGGTAAGGTGAGGTACGTGAGTAGAATTGGTCTGCTGTGGGAACATGTAAGGAGGAACGAAATTGAGGTTCCAGAATTCATTTATGATCATGATGAAGATCTTGAGTACCGTCCGATGATGGACTATGGTCAAGAGAGTGACCATCCTAGGGTTTACGGTGCACCCTCAATGGAATCCCACGTGTACTCCATGAGGTGCATTTCGTAG

mRNA sequence

AATTAATTTTATGAAAAAGGGATTTAACCGATATTAAAAAGCAGAGGTGTCATGTGGAGAGGGAAGGAGGGTAACAGAATATTCGTCTGTGACAAAAATCTTTGACCCACGTCGGCGCCATTTTATCGGCCACCATGATCTTCATTTCTTTCCACCTTTGTTAAAAATTCGATTCAATTCAATTCAATTATTCATTCTTCGTTCTTAATTTATTTTCCAAATCTCGTTCGACCAATTCCATCTCCACCAAATCCGTTCAATCCATCCTTATCCATATCCGTAGTTTGCCCTAACTATGATCTGCACGCTACTCCCTTTTTCCTTCTGATTCTAATCGGAGTTCTTCAACTATGTCCGCTCAGGTTCAGCCTCCTCCGCCGGAGCAAAACCAACTCGAAATGTTCGATGTCTTCAAGATCAAAGGCAGGGACAAACAGTGCCGCCGGATTCTCCGTATCGTCGGAAAATTCTTTCCAGCGCGGACTGTGAGTTTGGATGTGGTGAAGAATCATCTGGAGGAGAAGATCTTCCCTAGGCTTAAGAACAAGCGTTTCACGATCCTGTACTTTCACACCAGCGTTCAAAGGAGCCAAAATTTCCCCGGAATCGCCGCTCTCCGATCGATCTATGACGCAATTCCGGCCGCCGTCAAGGCTAATCTGGAAGCCGTGTATTTCGTTCACCCAGACCTCCAAGCCAGGCTCTTCCTCGCCACTCTCGGCCGCATTTTCTTCAGCAGCGAGGTGTATGGTAAGGTGAGGTACGTGAGTAGAATTGGTCTGCTGTGGGAACATGTAAGGAGGAACGAAATTGAGGTTCCAGAATTCATTTATGATCATGATGAAGATCTTGAGTACCGTCCGATGATGGACTATGGTCAAGAGAGTGACCATCCTAGGGTTTACGGTGCACCCTCAATGGAATCCCACGTGTACTCCATGAGGTGCATTTCGTAG

Coding sequence (CDS)

ATGTCCGCTCAGGTTCAGCCTCCTCCGCCGGAGCAAAACCAACTCGAAATGTTCGATGTCTTCAAGATCAAAGGCAGGGACAAACAGTGCCGCCGGATTCTCCGTATCGTCGGAAAATTCTTTCCAGCGCGGACTGTGAGTTTGGATGTGGTGAAGAATCATCTGGAGGAGAAGATCTTCCCTAGGCTTAAGAACAAGCGTTTCACGATCCTGTACTTTCACACCAGCGTTCAAAGGAGCCAAAATTTCCCCGGAATCGCCGCTCTCCGATCGATCTATGACGCAATTCCGGCCGCCGTCAAGGCTAATCTGGAAGCCGTGTATTTCGTTCACCCAGACCTCCAAGCCAGGCTCTTCCTCGCCACTCTCGGCCGCATTTTCTTCAGCAGCGAGGTGTATGGTAAGGTGAGGTACGTGAGTAGAATTGGTCTGCTGTGGGAACATGTAAGGAGGAACGAAATTGAGGTTCCAGAATTCATTTATGATCATGATGAAGATCTTGAGTACCGTCCGATGATGGACTATGGTCAAGAGAGTGACCATCCTAGGGTTTACGGTGCACCCTCAATGGAATCCCACGTGTACTCCATGAGGTGCATTTCGTAG

Protein sequence

MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIFPRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESDHPRVYGAPSMESHVYSMRCIS

Homology

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BLAST of Carg01191 vs. NCBI nr
Match: KAG7027813.1 (Protein GDAP2-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 408.3 bits (1048), Expect = 3.8e-110
Identity = 201/201 (100.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF
Sbjct: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD
Sbjct: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPSMESHVYSMRCIS
Sbjct: 181 HPRVYGAPSMESHVYSMRCIS 201

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BLAST of Carg01191 vs. NCBI nr
Match: XP_022959238.1 (ganglioside-induced differentiation-associated protein 2-like [Cucurbita moschata] >XP_022959320.1 ganglioside-induced differentiation-associated protein 2-like [Cucurbita moschata])

HSP 1 Score: 406.0 bits (1042), Expect = 1.9e-109
Identity = 199/201 (99.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF
Sbjct: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKN+RFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNRRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD
Sbjct: 121 ATLGRIFFTSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPSMESHVYSMRCIS
Sbjct: 181 HPRVYGAPSMESHVYSMRCIS 201

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BLAST of Carg01191 vs. NCBI nr
Match: XP_022971318.1 (ganglioside-induced differentiation-associated protein 2-like [Cucurbita maxima])

HSP 1 Score: 403.3 bits (1035), Expect = 1.2e-108
Identity = 197/201 (98.01%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MSAQ+QPPPPEQNQLE FDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF
Sbjct: 1   MSAQIQPPPPEQNQLEKFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQAR+FL
Sbjct: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARVFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD
Sbjct: 121 ATLGRIFFTSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPSMESHVYSMRCIS
Sbjct: 181 HPRVYGAPSMESHVYSMRCIS 201

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BLAST of Carg01191 vs. NCBI nr
Match: XP_023540079.1 (ganglioside-induced differentiation-associated protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 402.9 bits (1034), Expect = 1.6e-108
Identity = 198/201 (98.51%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MSAQVQPPPPEQNQLE FDVFKIKGRDKQCRRILRIVGKFFPARTVSL+VVKNHLEEKIF
Sbjct: 1   MSAQVQPPPPEQNQLEKFDVFKIKGRDKQCRRILRIVGKFFPARTVSLNVVKNHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD
Sbjct: 121 ATLGRIFFTSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPSMESHVYSMRCIS
Sbjct: 181 HPRVYGAPSMESHVYSMRCIS 201

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BLAST of Carg01191 vs. NCBI nr
Match: XP_022923521.1 (ganglioside-induced differentiation-associated protein 2-like [Cucurbita moschata] >XP_023007626.1 ganglioside-induced differentiation-associated protein 2-like [Cucurbita maxima] >KAG6577807.1 Protein GDAP2-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015847.1 Protein GDAP2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.8 bits (961), Expect = 4.7e-100
Identity = 183/201 (91.04%), Postives = 191/201 (95.02%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           M+AQVQPPPPEQNQLE  DVFKIKGRDKQ RRILRI GKFFPAR VSLDVVK HLEEKIF
Sbjct: 1   MAAQVQPPPPEQNQLENLDVFKIKGRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHT VQRSQNFPGIAALRSIY+AIPA VKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNKRFTILYFHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SE+YGK++YVSRI LLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYG ESD
Sbjct: 121 ATLGRIFFTSEMYGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPS++SHVYSMRCIS
Sbjct: 181 HPRVYGAPSIDSHVYSMRCIS 201

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BLAST of Carg01191 vs. ExPASy Swiss-Prot
Match: A7T167 (Protein GDAP2 homolog OS=Nematostella vectensis OX=45351 GN=gdap2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.2e-09
Identity = 35/140 (25.00%), Postives = 73/140 (52.14%), Query Frame = 0


Query: 25  GRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIFPRLKNKRFTILYFHTSVQRSQNFP 84
           G D   R ++  V + F A+   L     +    +  R+ N+ + ++YFHT     +N P
Sbjct: 346 GVDFLGRPVVVFVARHFTAQNTDLGKAVAYF-ISVLDRIVNRDYVVVYFHTH-STEENQP 405

Query: 85  GIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGRIFFSSEVYGKVRYVSRIGL 144
            ++ L+ +Y  +    + NL+A Y VHP + AR+ +      F +S V  KV ++S +  
Sbjct: 406 PMSFLKELYHIVDNKYRRNLKAFYIVHPTVWARI-VTWFFTTFTASSVKEKVHFLSGVQY 465

Query: 145 LWEHVRRNEIEVPEFIYDHD 165
           L++ +  +++++P ++ ++D
Sbjct: 466 LYDWINPDQLDIPAYVLEYD 482

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BLAST of Carg01191 vs. ExPASy Swiss-Prot
Match: Q0IHU9 (Protein prune homolog 2 OS=Xenopus tropicalis OX=8364 GN=Prune2 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 7.0e-06
Identity = 35/122 (28.69%), Postives = 60/122 (49.18%), Query Frame = 0


Query: 68  FTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGRIF 127
           + ++Y + +  R +  PG+  ++  Y  I   ++ NL++   VHP    R  LA L R F
Sbjct: 187 YMVVYLNGATPR-RKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILA-LTRPF 246

Query: 128 FSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESDHPRVYGA 187
            SS+   K++YVS +  L E +    + +PE I   DE+L         +ES+ P+    
Sbjct: 247 ISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKLDEEL---------RESESPKAGCL 297

Query: 188 PS 190
           P+
Sbjct: 307 PN 297

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BLAST of Carg01191 vs. ExPASy Swiss-Prot
Match: Q7Z465 (Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein OS=Homo sapiens OX=9606 GN=BNIPL PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 3.0e-04
Identity = 27/102 (26.47%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 66  KRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGR 125
           + + +++      R+Q  P ++ +R  Y  +   ++ NL A+  VH     + FLA L R
Sbjct: 252 ENYLLVHLSGGTSRAQ-VPPLSWIRQCYRTLDRRLRKNLRALVVVHATWYVKAFLALL-R 311

Query: 126 IFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDL 168
            F SS+   K+R++  +G L + +  +++ +PE +   D DL
Sbjct: 312 PFISSKFTRKIRFLDSLGELAQLISLDQVHIPEAVRQLDRDL 351

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BLAST of Carg01191 vs. ExPASy Swiss-Prot
Match: Q99JU7 (Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein OS=Mus musculus OX=10090 GN=Bnipl PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 5.0e-04
Identity = 27/102 (26.47%), Postives = 52/102 (50.98%), Query Frame = 0


Query: 66  KRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGR 125
           + + +++      R+Q  P ++ +R  Y  +   ++ NL A+  VH     + FLA L R
Sbjct: 223 ENYLLVHLSGGTSRAQ-VPPLSWIRQCYRTLDRRLRKNLRALVVVHATWYVKAFLA-LVR 282

Query: 126 IFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDL 168
            F SS+   K+R++  +G L + +   ++ +PE +   D DL
Sbjct: 283 PFISSKFTRKIRFLDSLGELAQLISLEQVHIPEVVRQLDRDL 322

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BLAST of Carg01191 vs. ExPASy TrEMBL
Match: A0A6J1H5D8 (ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460261 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 9.2e-110
Identity = 199/201 (99.00%), Postives = 201/201 (100.00%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF
Sbjct: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKN+RFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNRRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD
Sbjct: 121 ATLGRIFFTSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPSMESHVYSMRCIS
Sbjct: 181 HPRVYGAPSMESHVYSMRCIS 201

[Close]

BLAST of Carg01191 vs. ExPASy TrEMBL
Match: A0A6J1I6H6 (ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470075 PE=4 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 6.0e-109
Identity = 197/201 (98.01%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MSAQ+QPPPPEQNQLE FDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF
Sbjct: 1   MSAQIQPPPPEQNQLEKFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQAR+FL
Sbjct: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARVFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD
Sbjct: 121 ATLGRIFFTSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPSMESHVYSMRCIS
Sbjct: 181 HPRVYGAPSMESHVYSMRCIS 201

[Close]

BLAST of Carg01191 vs. ExPASy TrEMBL
Match: A0A6J1L134 (ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111500201 PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 2.3e-100
Identity = 183/201 (91.04%), Postives = 191/201 (95.02%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           M+AQVQPPPPEQNQLE  DVFKIKGRDKQ RRILRI GKFFPAR VSLDVVK HLEEKIF
Sbjct: 1   MAAQVQPPPPEQNQLENLDVFKIKGRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHT VQRSQNFPGIAALRSIY+AIPA VKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNKRFTILYFHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SE+YGK++YVSRI LLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYG ESD
Sbjct: 121 ATLGRIFFTSEMYGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPS++SHVYSMRCIS
Sbjct: 181 HPRVYGAPSIDSHVYSMRCIS 201

[Close]

BLAST of Carg01191 vs. ExPASy TrEMBL
Match: A0A6J1E710 (ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431194 PE=4 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 2.3e-100
Identity = 183/201 (91.04%), Postives = 191/201 (95.02%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           M+AQVQPPPPEQNQLE  DVFKIKGRDKQ RRILRI GKFFPAR VSLDVVK HLEEKIF
Sbjct: 1   MAAQVQPPPPEQNQLENLDVFKIKGRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHT VQRSQNFPGIAALRSIY+AIPA VKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNKRFTILYFHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SE+YGK++YVSRI LLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYG ESD
Sbjct: 121 ATLGRIFFTSEMYGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPS++SHVYSMRCIS
Sbjct: 181 HPRVYGAPSIDSHVYSMRCIS 201

[Close]

BLAST of Carg01191 vs. ExPASy TrEMBL
Match: A0A0A0L0Z7 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002450 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.1e-100
Identity = 184/201 (91.54%), Postives = 189/201 (94.03%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           M+A + PPPPEQN LE  DVFKIKGRDKQ RRILRI GKFFPAR VSLDV+K HLEEKIF
Sbjct: 1   MAAHLPPPPPEQNYLEKLDVFKIKGRDKQGRRILRITGKFFPARVVSLDVLKKHLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRLKNKRFTILYFHT VQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL
Sbjct: 61  PRLKNKRFTILYFHTGVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           ATLGRIFF+SEVYGKVRYVSRI LLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYG ESD
Sbjct: 121 ATLGRIFFTSEVYGKVRYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESD 180

Query: 181 HPRVYGAPSMESHVYSMRCIS 202
           HPRVYGAPS+ESHVYSMRCIS
Sbjct: 181 HPRVYGAPSVESHVYSMRCIS 201

[Close]

BLAST of Carg01191 vs. TAIR 10
Match: AT4G35750.1 (SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein )

HSP 1 Score: 275.8 bits (704), Expect = 2.8e-74
Identity = 134/203 (66.01%), Postives = 165/203 (81.28%), Query Frame = 0


Query: 1   MSAQVQPPPPEQNQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIF 60
           MS+Q+     EQ  +E  ++FKI GRDK+ R+ILRI+GKFFPAR +SLDV+K +LEEKIF
Sbjct: 1   MSSQISEIEQEQ-LIEKLEIFKIHGRDKRGRKILRIIGKFFPARFLSLDVLKKYLEEKIF 60

Query: 61  PRLKNKRFTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFL 120
           PRL  K F +LY HT VQRS+NFPGI+ALR+IYDAIP  V+ NL+ VYF+HP LQ+RLFL
Sbjct: 61  PRLGRKPFAVLYVHTGVQRSENFPGISALRAIYDAIPVNVRDNLQEVYFLHPGLQSRLFL 120

Query: 121 ATLGRIFFSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 180
           AT GR  FS  +YGK+RY+SR+  LWEHVRRNEIE+PEF+YDHD+DLEYRPMMDYGQESD
Sbjct: 121 ATCGRFLFSGGLYGKLRYISRVDYLWEHVRRNEIEMPEFVYDHDDDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSMESHV--YSMRCIS 202
           H RV+   +++S V  +SMRCIS
Sbjct: 181 HARVFAGAAVDSSVSSFSMRCIS 202

[Close]

BLAST of Carg01191 vs. TAIR 10
Match: AT3G10210.1 (SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein )

HSP 1 Score: 165.6 bits (418), Expect = 4.0e-41
Identity = 73/168 (43.45%), Postives = 115/168 (68.45%), Query Frame = 0


Query: 13  NQLEMFDVFKIKGRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIFPRLKNKRFTILY 72
           + L++   F ++G D+   RI RIVGK+FPAR VS + +K ++ +KI  +       ++Y
Sbjct: 47  SDLDLLQFFTLQGLDRSGNRIFRIVGKYFPARVVSAERLKKYISQKISNQCPEGPLCLVY 106

Query: 73  FHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGRIFFSSEV 132
            H++VQ+  N PGI  LR IY+ +P+ +K  L+ VYF+HP L++RL +ATLGR+  S  +
Sbjct: 107 MHSTVQKDDNSPGITILRWIYEDLPSDIKDRLQLVYFIHPGLRSRLVIATLGRLLLSGGL 166

Query: 133 YGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGQESD 181
           Y K++YVSR+  LWE +++ E+E+P+F+ +HD  LE+RP+ DYG E D
Sbjct: 167 YWKIKYVSRLQYLWEDIKKGEVEIPDFVKNHDNVLEHRPLTDYGIEPD 214

[Close]

BLAST of Carg01191 vs. TAIR 10
Match: AT1G69340.1 (appr-1-p processing enzyme family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.2e-10
Identity = 38/157 (24.20%), Postives = 84/157 (53.50%), Query Frame = 0


Query: 13  NQLEMFDVFKIK-----GRDKQCRRILRIVGKFFPARTVSLDVVKNHLEEKIFPRLKNKR 72
           N + + ++ ++K     G D +   ++ +VG  F  R + L+    ++ ++  P ++ K 
Sbjct: 387 NSINLSEIAEMKIVYRGGVDTEGHPVMVVVGAHFLLRCLDLERFVLYVIKEFEPVIQ-KP 446

Query: 73  FTILYFHTSVQRSQNFPGIAALRSIYDAIPAAVKANLEAVYFVHPDLQARLFLATLGRIF 132
           ++I+YFH S    Q  P +  ++ +   +    + NL+A+Y +HP    +  + T+ + F
Sbjct: 447 YSIVYFH-SAASLQVQPDLGWMKRLEQILGRKHQRNLQAIYVLHPTFHLKATILTM-QFF 506

Query: 133 FSSEVYGKVRYVSRIGLLWEHVRRNEIEVPEFIYDHD 165
             + V+ KV Y  R+  L+++V R ++ +P+F++ HD
Sbjct: 507 VDNVVWKKVVYADRLLQLFKYVPREQLTIPDFVFQHD 540

The following BLAST results are available for this feature:

BLAST of Carg01191 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027813.1	3.8e-110	100.00	Protein GDAP2-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022959238.1	1.9e-109	99.00	ganglioside-induced differentiation-associated protein 2-like [Cucurbita moschat...	[more]
XP_022971318.1	1.2e-108	98.01	ganglioside-induced differentiation-associated protein 2-like [Cucurbita maxima]	[more]
XP_023540079.1	1.6e-108	98.51	ganglioside-induced differentiation-associated protein 2-like [Cucurbita pepo su...	[more]
XP_022923521.1	4.7e-100	91.04	ganglioside-induced differentiation-associated protein 2-like [Cucurbita moschat...	[more]

BLAST of Carg01191 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7T167	5.2e-09	25.00	Protein GDAP2 homolog OS=Nematostella vectensis OX=45351 GN=gdap2 PE=3 SV=1	[more]
Q0IHU9	7.0e-06	28.69	Protein prune homolog 2 OS=Xenopus tropicalis OX=8364 GN=Prune2 PE=2 SV=1	[more]
Q7Z465	3.0e-04	26.47	Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein OS=Homo sapiens O...	[more]
Q99JU7	5.0e-04	26.47	Bcl-2/adenovirus E1B 19 kDa-interacting protein 2-like protein OS=Mus musculus O...	[more]

BLAST of Carg01191 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H5D8	9.2e-110	99.00	ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita mosch...	[more]
A0A6J1I6H6	6.0e-109	98.01	ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita maxim...	[more]
A0A6J1L134	2.3e-100	91.04	ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita maxim...	[more]
A0A6J1E710	2.3e-100	91.04	ganglioside-induced differentiation-associated protein 2-like OS=Cucurbita mosch...	[more]
A0A0A0L0Z7	5.1e-100	91.54	CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002450 P...	[more]

BLAST of Carg01191 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35750.1	2.8e-74	66.01	SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein...	[more]
AT3G10210.1	4.0e-41	43.45	SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein...	[more]
AT1G69340.1	2.2e-10	24.20	appr-1-p processing enzyme family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001251	CRAL-TRIO lipid binding domain	SMART	SM00516	sec14_4	coord: 14..163 e-value: 2.3E-11 score: 53.8
IPR001251	CRAL-TRIO lipid binding domain	PFAM	PF13716	CRAL_TRIO_2	coord: 31..166 e-value: 1.1E-28 score: 100.1
IPR001251	CRAL-TRIO lipid binding domain	CDD	cd00170	SEC14	coord: 25..157 e-value: 1.14771E-8 score: 50.0762
IPR036865	CRAL-TRIO lipid binding domain superfamily	GENE3D	3.40.525.10		coord: 14..168 e-value: 4.6E-24 score: 86.7
None	No IPR available	PANTHER	PTHR11106	GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATED	coord: 10..201
None	No IPR available	PANTHER	PTHR11106:SF103	OS04G0561200 PROTEIN	coord: 10..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01191-RA	Carg01191-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane