Carg00496 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00496
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	major latex allergen Hev b 5-like
Location	Carg_Chr03: 5314822 .. 5315634 (+)
RNA-Seq Expression	Carg00496
Synteny	Carg00496

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTATATAAACCCTCTCTCTCTGCTGCTTCTTTCCATCAACTCGCTCTCTCTCAGTATTTTAGTTTCTTCTAAGCTTAATTCTCTTCTTCTCTTCCATATTGCTTCTTCTTCCCTTTCCCAAATGGCCACCATTGAGGTAATTTCAAGCTTTTATAGCTTTCTCTCATGGCTTTAAGATCTTTTTTCTTGTTTTTCTTTTGGTATTCTAACAATGGCTGTATGAGTGTGATCATGTTGTTACTGATTTAATGATCTGTGGGGTATTGGATCACATTCTCATATGAATATGATCATTGAAGGCTGTTTGAACATATTGTGTTTGAATGGAAATCCATAATGAATTGTGCGATCTGGGTGTGGCAGGTTGAATCAGCCCCCGTTGTATTGGCAGAGCCTGAGGCAGCTCCAGTGGTCCAAGCTGAGGAAAAAGTTGCAGAGGAGGCAGTGAGTGAAGCAGCCCCTGCAGAAGAGGCAAAGGAGGAGGAAGCTGCCTCTGAAGCTGTTGAGACAGAGGAACCAGCACCGGTCGCAGCCGAACCCGAGGTCGAAACCAAGGAAGTTGTGGTAGAGGAGGCAGTGGAGGCAGAGCCTGCAGCTGCTGCTGCTTCCGAGCCCGAAGCAGCGGCCGAGGCGGAAGAGAAGAAGGAAGAGACCAAAGAGGAAACAGAAGAGCCAGTGGTAGAGAAAAAAGAAGAAGAAGAAGAACCTCAGGAAGTGAAGGCTGAAGAGACTGTTGAAGAGAGCTCAGAAGCAGCAGCAGCAGCAGCAGCAGCAGCACCAGCTGAGGAAGAGAAGCCAAAGGAAGAGGAATAA

mRNA sequence

CTATATAAACCCTCTCTCTCTGCTGCTTCTTTCCATCAACTCGCTCTCTCTCAGTATTTTAGTTTCTTCTAAGCTTAATTCTCTTCTTCTCTTCCATATTGCTTCTTCTTCCCTTTCCCAAATGGCCACCATTGAGGTTGAATCAGCCCCCGTTGTATTGGCAGAGCCTGAGGCAGCTCCAGTGGTCCAAGCTGAGGAAAAAGTTGCAGAGGAGGCAGTGAGTGAAGCAGCCCCTGCAGAAGAGGCAAAGGAGGAGGAAGCTGCCTCTGAAGCTGTTGAGACAGAGGAACCAGCACCGGTCGCAGCCGAACCCGAGGTCGAAACCAAGGAAGTTGTGGTAGAGGAGGCAGTGGAGGCAGAGCCTGCAGCTGCTGCTGCTTCCGAGCCCGAAGCAGCGGCCGAGGCGGAAGAGAAGAAGGAAGAGACCAAAGAGGAAACAGAAGAGCCAGTGGTAGAGAAAAAAGAAGAAGAAGAAGAACCTCAGGAAGTGAAGGCTGAAGAGACTGTTGAAGAGAGCTCAGAAGCAGCAGCAGCAGCAGCAGCAGCAGCACCAGCTGAGGAAGAGAAGCCAAAGGAAGAGGAATAA

Coding sequence (CDS)

ATGGCCACCATTGAGGTTGAATCAGCCCCCGTTGTATTGGCAGAGCCTGAGGCAGCTCCAGTGGTCCAAGCTGAGGAAAAAGTTGCAGAGGAGGCAGTGAGTGAAGCAGCCCCTGCAGAAGAGGCAAAGGAGGAGGAAGCTGCCTCTGAAGCTGTTGAGACAGAGGAACCAGCACCGGTCGCAGCCGAACCCGAGGTCGAAACCAAGGAAGTTGTGGTAGAGGAGGCAGTGGAGGCAGAGCCTGCAGCTGCTGCTGCTTCCGAGCCCGAAGCAGCGGCCGAGGCGGAAGAGAAGAAGGAAGAGACCAAAGAGGAAACAGAAGAGCCAGTGGTAGAGAAAAAAGAAGAAGAAGAAGAACCTCAGGAAGTGAAGGCTGAAGAGACTGTTGAAGAGAGCTCAGAAGCAGCAGCAGCAGCAGCAGCAGCAGCACCAGCTGAGGAAGAGAAGCCAAAGGAAGAGGAATAA

Protein sequence

MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPVAAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE

Homology

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BLAST of Carg00496 vs. NCBI nr
Match: KAG6603817.1 (hypothetical protein SDJN03_04426, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033998.1 hypothetical protein SDJN02_03724 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 219.2 bits (557), Expect = 2.5e-53
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV
Sbjct: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60

Query: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120
           AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP
Sbjct: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120

Query: 121 QEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
           QEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE
Sbjct: 121 QEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 154

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BLAST of Carg00496 vs. NCBI nr
Match: XP_022950569.1 (major latex allergen Hev b 5-like [Cucurbita moschata])

HSP 1 Score: 214.5 bits (545), Expect = 6.2e-52
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV
Sbjct: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60

Query: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120
           AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP
Sbjct: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120

Query: 121 QEVKAEETVEESSE-AAAAAAAAAPAEEEKPKEEE 155
           QEVKAEETVEESSE AAAAAAAAAPAEEEKPKEEE
Sbjct: 121 QEVKAEETVEESSEAAAAAAAAAAPAEEEKPKEEE 155

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BLAST of Carg00496 vs. NCBI nr
Match: XP_023544677.1 (uncharacterized protein LOC111804190 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023544678.1 uncharacterized protein LOC111804190 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.9 bits (481), Expect = 1.6e-44
Identity = 146/159 (91.82%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVLAEPEAAPVVQAEEKV EE VSEAAPAEE KEEEAASEAVETEEPAPV
Sbjct: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVVEEPVSEAAPAEEPKEEEAASEAVETEEPAPV 60

Query: 61  AAEPEVETKEVVVE---EAVEAEPA--AAAASEPEAAAEAEEKKEETKEETEEPVVEKKE 120
           AAEPEVETKEVVVE   EAVEAEPA  AAAASEPEA A AEEKKEETKEETEEPVVEKKE
Sbjct: 61  AAEPEVETKEVVVEEAAEAVEAEPAAPAAAASEPEAPAGAEEKKEETKEETEEPVVEKKE 120

Query: 121 EEEEPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
           EEEEPQEVKAE+TVEESSE AAAAAA APAEEEKPKEEE
Sbjct: 121 EEEEPQEVKAEDTVEESSE-AAAAAAPAPAEEEKPKEEE 158

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BLAST of Carg00496 vs. NCBI nr
Match: XP_022979119.1 (major latex allergen Hev b 5-like [Cucurbita maxima])

HSP 1 Score: 183.7 bits (465), Expect = 1.2e-42
Identity = 144/160 (90.00%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVL EPEAAPVVQAEEKVAEEAVSEAAPAEE KEEEAASEAVETEEPAPV
Sbjct: 1   MATIEVESAPVVLPEPEAAPVVQAEEKVAEEAVSEAAPAEEPKEEEAASEAVETEEPAPV 60

Query: 61  AAEPEVETKEVVVE---EAVEAEP---AAAAASEPEAAAEAEEKKEETKEETEEPVVEKK 120
           A EPEVETKEVVVE   EAVEAEP   AAAAASEPEAAAEAEEKKEETKEETEEPVVEKK
Sbjct: 61  ATEPEVETKEVVVEEAAEAVEAEPAAAAAAAASEPEAAAEAEEKKEETKEETEEPVVEKK 120

Query: 121 EEEEEPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
            EEEEPQEVKAEETVEESSE      AAAPAEEEKPKEEE
Sbjct: 121 -EEEEPQEVKAEETVEESSE-----EAAAPAEEEKPKEEE 154

[Close]

BLAST of Carg00496 vs. NCBI nr
Match: XP_038880819.1 (probable serine/threonine-protein kinase kinX [Benincasa hispida])

HSP 1 Score: 122.5 bits (306), Expect = 3.2e-24
Identity = 112/156 (71.79%), Postives = 121/156 (77.56%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVL EPEAAPVVQ EEKV EE V+E APAEE K EEAA EAV+ EEPAPV
Sbjct: 1   MATIEVESAPVVLPEPEAAPVVQTEEKVVEEPVAE-APAEEPK-EEAAPEAVKAEEPAPV 60

Query: 61  AAEPEVETKEVVVEEA--VEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEE 120
           A EP+VETKEVV EE    EAEP A  ASEPEA  EAEEK+EE KEET    VE+K EEE
Sbjct: 61  ATEPDVETKEVVEEETKPEEAEP-AVGASEPEAPVEAEEKEEEAKEET----VEEKTEEE 120

Query: 121 EPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
           +PQEVK+E+ VEE S     + AAAPAEEEKPK EE
Sbjct: 121 KPQEVKSEDVVEEES-----SEAAAPAEEEKPKAEE 144

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BLAST of Carg00496 vs. ExPASy TrEMBL
Match: A0A6J1GG70 (major latex allergen Hev b 5-like OS=Cucurbita moschata OX=3662 GN=LOC111453630 PE=4 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 3.0e-52
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV
Sbjct: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60

Query: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120
           AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP
Sbjct: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120

Query: 121 QEVKAEETVEESSE-AAAAAAAAAPAEEEKPKEEE 155
           QEVKAEETVEESSE AAAAAAAAAPAEEEKPKEEE
Sbjct: 121 QEVKAEETVEESSEAAAAAAAAAAPAEEEKPKEEE 155

[Close]

BLAST of Carg00496 vs. ExPASy TrEMBL
Match: A0A6J1IVT4 (major latex allergen Hev b 5-like OS=Cucurbita maxima OX=3661 GN=LOC111478851 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 5.7e-43
Identity = 144/160 (90.00%), Postives = 144/160 (90.00%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVL EPEAAPVVQAEEKVAEEAVSEAAPAEE KEEEAASEAVETEEPAPV
Sbjct: 1   MATIEVESAPVVLPEPEAAPVVQAEEKVAEEAVSEAAPAEEPKEEEAASEAVETEEPAPV 60

Query: 61  AAEPEVETKEVVVE---EAVEAEP---AAAAASEPEAAAEAEEKKEETKEETEEPVVEKK 120
           A EPEVETKEVVVE   EAVEAEP   AAAAASEPEAAAEAEEKKEETKEETEEPVVEKK
Sbjct: 61  ATEPEVETKEVVVEEAAEAVEAEPAAAAAAAASEPEAAAEAEEKKEETKEETEEPVVEKK 120

Query: 121 EEEEEPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
            EEEEPQEVKAEETVEESSE      AAAPAEEEKPKEEE
Sbjct: 121 -EEEEPQEVKAEETVEESSE-----EAAAPAEEEKPKEEE 154

[Close]

BLAST of Carg00496 vs. ExPASy TrEMBL
Match: A0A5D3CMF4 (Fibrous sheath CABYR-binding protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005990 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 8.9e-20
Identity = 102/156 (65.38%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVL EPEAAP VQ EEKV EE V+E  PA+E KEE A     E E+PAPV
Sbjct: 1   MATIEVESAPVVLPEPEAAPAVQPEEKVVEEPVAEETPAQEQKEEAAPEPVEEVEKPAPV 60

Query: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPE--AAAEAEEKKEETKEETEEPVVEKKEEEE 120
             EP VETKE VVEE       A  A+EPE  A AEAE+K+EETKEET E V EK   EE
Sbjct: 61  ETEPVVETKEDVVEEKESKPEEAEPAAEPEAPAEAEAEKKEEETKEETVEAVEEK--AEE 120

Query: 121 EPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
           E +EVK+EE VEESSE      AAAPAEEEKPK EE
Sbjct: 121 EAKEVKSEEVVEESSE------AAAPAEEEKPKAEE 148

[Close]

BLAST of Carg00496 vs. ExPASy TrEMBL
Match: A0A0A0KMX7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511710 PE=4 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 2.0e-19
Identity = 101/156 (64.74%), Postives = 111/156 (71.15%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MAT+EVES PVVL EPEAAP VQ EEKV EE V+E  PA+E +EE AA  A E  E APV
Sbjct: 1   MATVEVESVPVVLPEPEAAPAVQPEEKVVEEPVAEETPAQEQEEEAAAPAAPEPVEEAPV 60

Query: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPE--AAAEAEEKKEETKEETEEPVVEKKEEEE 120
           A EPEVETKE VVEE       A  A+EPE  A AEAE+K+EETKEET E V EK   EE
Sbjct: 61  ATEPEVETKEEVVEEKESKPEEAEPAAEPEAPAEAEAEKKEEETKEETVEAVEEK--TEE 120

Query: 121 EPQEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
           E +EVK+E+ VEESSE      AAAPAEEEKPK EE
Sbjct: 121 ETKEVKSEDAVEESSE------AAAPAEEEKPKAEE 148

[Close]

BLAST of Carg00496 vs. ExPASy TrEMBL
Match: A0A1S3B0W0 (fibrous sheath CABYR-binding protein-like OS=Cucumis melo OX=3656 GN=LOC103484659 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 7.5e-19
Identity = 99/154 (64.29%), Postives = 108/154 (70.13%), Query Frame = 0


Query: 1   MATIEVESAPVVLAEPEAAPVVQAEEKVAEEAVSEAAPAEEAKEEEAASEAVETEEPAPV 60
           MATIEVESAPVVL EPEAAP VQ EEKV EE V+E  PA+E KEE A     E E+PAPV
Sbjct: 1   MATIEVESAPVVLPEPEAAPAVQPEEKVVEEPVAEETPAQEQKEEAAPEPVEEVEKPAPV 60

Query: 61  AAEPEVETKEVVVEEAVEAEPAAAAASEPEAAAEAEEKKEETKEETEEPVVEKKEEEEEP 120
             EP VETKE VVEE       A  A+EPEA AEAE   EE +EET+E  VE+K  EEE 
Sbjct: 61  ETEPVVETKEEVVEEKESKPEEAEPAAEPEAPAEAE--AEEKEEETKEEAVEEK-AEEEA 120

Query: 121 QEVKAEETVEESSEAAAAAAAAAPAEEEKPKEEE 155
           +EVK+EE VEESSE      AAAPAEEEKPK EE
Sbjct: 121 KEVKSEEVVEESSE------AAAPAEEEKPKAEE 145

The following BLAST results are available for this feature:

BLAST of Carg00496 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6603817.1	2.5e-53	100.00	hypothetical protein SDJN03_04426, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022950569.1	6.2e-52	99.35	major latex allergen Hev b 5-like [Cucurbita moschata]	[more]
XP_023544677.1	1.6e-44	91.82	uncharacterized protein LOC111804190 isoform X1 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_022979119.1	1.2e-42	90.00	major latex allergen Hev b 5-like [Cucurbita maxima]	[more]
XP_038880819.1	3.2e-24	71.79	probable serine/threonine-protein kinase kinX [Benincasa hispida]	[more]

BLAST of Carg00496 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg00496 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GG70	3.0e-52	99.35	major latex allergen Hev b 5-like OS=Cucurbita moschata OX=3662 GN=LOC111453630 ...	[more]
A0A6J1IVT4	5.7e-43	90.00	major latex allergen Hev b 5-like OS=Cucurbita maxima OX=3661 GN=LOC111478851 PE...	[more]
A0A5D3CMF4	8.9e-20	65.38	Fibrous sheath CABYR-binding protein-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A0A0KMX7	2.0e-19	64.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G511710 PE=4 SV=1	[more]
A0A1S3B0W0	7.5e-19	64.29	fibrous sheath CABYR-binding protein-like OS=Cucumis melo OX=3656 GN=LOC10348465...	[more]

BLAST of Carg00496 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 30..45
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 30..154
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 91..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00496-RA	Carg00496-RA	mRNA