Carg00473 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00473
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein TIFY 8-like
LocationCarg_Chr04: 7486761 .. 7490716 (-)
RNA-Seq ExpressionCarg00473
SyntenyCarg00473
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCTTTCCAATTCAGAAAGCTGTCCTCATCCATCAAATTTTTCATCATTGATTTTTCTCTCAACAATTCCTTTCTCGATGTGATTTTGATTTTCATGAGCTCGAGGCCGGTTTGATTTTCGTTCGCGCGCAACCAAATTGACGGCCACGAACCAACGAATTCTGTGAATTTTTCCATTATTTAATCAAATCCGATCCGGTGAACGGAGGAAACTGAATCCCTCACCGGCGTCGGCGTCGGAAATTTTCTATAGAATCTTTTATCTCGAGAAAAGAGCCTGATTCTCTCTCTCTTCTGATTCTTTTGGCCTCTTTTTTCTGTATGTTTCTTTTCAAAAATCGTGTTGCTTGTCTTTTGTTTTTCTCTACACTCTTGCTCTTTATGCAGAGTGAAATGGATGCTTTCGTAAAGAGACAACTCTTGAGGTTCCATCTCTAATGGCTCATTCCAATGCGGGCTACTGCAGCAATGGCGAGAAGAAGAGCACGAGGCAGGTTCAACCACTAGGTAATGAGATCTTCCATGATTTTCTTGGCATTAGGACCAAGGATGCTTCTGTACTCTCGGCCGCGAAGACGCCGGATATAACCTTATCGGAAGCTTCGTCGCCGTCGTCGGCCTTGGCTTCTTCTAGCGGCGGCCGTGGACTCCTCTCCGCCGCTTCCGATCTTGCTTCTGGTCAGTCTGTTAACATTCATCGTCTTCAATTTACTTCGTTCTAGGTTATTTATCGCTTGCTGGTTTTCGTCTCAACGATTTCGTCGTGGCCTGAATAACTTCCAATCTTTGCGATTATCCTTTTGCTTGATGAATATTCCGATCATTATCGACCTCTCGATTGTTTGAATGATTCGTTTGTTTTTGGTTAAATTGTTTTGATTCCAAAATATTGATTTTCATGTGTGAACTTTTCTTTTCTTTCTGCAACGAAGAATTTCTGTCTCCTTTTGCTCTTTATGTCACTGTAAAGTTTAGTGGATTGGTTTGAAGCTTTCTGAAAGGCCTCTTTAGCCCTACTCTCTTTTTCCTTCAGTTTTCAAACACTAAGTGCTTTTCGGAAACAGAAACTACACTTAGTTTTTTCTACAATCTTCCTTTCTCCATTGTTAATTTCTTTGGTTTTTTGCTTTATTTCCAAGTTTCAGCTGTCTGATTCTGGATTTGAATTCTTATGGGATGTAATGTCAACCTCTTTCAGATATGAATCTATCTGATTCAGAGGGGAAGAACTTGCATCAGTATTTCAATTTCAATTCAAACATGGGTTTGATTCACACTCCATGTTCTGAGAAATCTTTACCATTTACACTGTTCTTCATTTTTGGTAGAAAATCAGGTGGGAGGTTATCTTGAAGGGGTTCCCTTCTGCAGTCCAAGGAATGAGAAATCTGGACCTGAAAAAAGTAATTGGATAGCAGGGATTAAACGCAGTAATCCAGATCCTGGTTTTGTTGGATCATATAGAAACCAAATTCCTCATGTGCCTTCTGACTCTCTTGAGAGGTCACATACAATGAAGGTTTTGACAGCTTTTAGCTTCCTTTAATCACATTCCAGGGAAGTGAAAAGGATTTTAATGGATCATTTGATCTGATTTTCTTTTAACTTGTATCAGATGCTACAAACTGGACCTGGTGGAGATCGACTGAGATACAATGATAACGAGGCAGGTTTTGACAGCTTTTAGTTTTCTTTAATCACATTCCAGGGAAGTGACAAGGATTTTAATGGATCATTTGATCTGATTTTCTCTTAACTTGTATCAGTCACTACAAACTGGACCTGGTGGAGATCGACCGAGATACAATGATAACGAGGCAGTTTACTACAGTATGAAGCCTCCAAAAATTGCTTCTTATTCCCTCACACAACACTCTCTTGGCACTAGATTTAATCCTAGTGTTACCAAATGGGAGAGGCCTATTCCATTGAACACGAGCTCGGCACAGAATTCTCCGCTCGGGATTCAGTTGATGCCTCGTGTACATCAAGTAGGTTCCAACTCTTCCAGAGAATTTAATGTTGCTCCTTCCAGTGTGTCTCAATCTGCTGCTGATGAAGGATCTAGAACTGGGATGAAAAGCCCTGGACTTTTGAGTAGCATTAATGTTGAAAATGATGGGAGACATTCTTCTCAAATGTTGCTAAGTTGTGACAAGCAGAAATCCAAGACTGAAGGTTTAGAATACAAATCTTCTAATCCTCCAAGGTAAATTACCGATCACGTATCGTTCTGTACAGTTCCGTTTTTCAACTGTTCTATGAAAAATTTAAGCATTGTGTTTAAATCTATCGTGACAGTCAGCAGGGACTTGATTCGAACAACACGAAGATGACTATTTTCTATGGTGGTCAAGCTCATGTTTTTGATGATGTCCCTCCAAACAAGGTACTTTTCCATATGGAAAGGGTCATTAGAATAATAAAAAGCTCATTGAGAACGGATCCAAAATAGCTACTTACCTAGAATTTAGCATGCTAGGAGATTCCTCGACAACCAAATGTGTCTGTAAGATTAGTCGAAGTGTATGCAAACTGGCTCGGGCACTCATGGGTATTGAATAAAAAAGTATTCATTATAGTGTCTTCATGTTTGCTTCGACTCTTGAACTGATAAGCTTGCCGTTCATGCTTCAACAAACTAGAATTTAGTATGTTCAACAAGGTACCTTTCCATATGGCAATAAGGGTCATTAGAATAATAAAGAGCTCATTGAGAATAGATCCAAGATAGCTACTTACCTAGAATTTAGATTAGTCGAAGCGCATGCAAGCTGGCACGGGCACTCATGGGTATTAAATAAAAAAGTATTCATTATAGTATCTTCATGTTTGCTTCGACTTTTGAACTGACAAGCTTGCTGTTCATGCTTCATACAAACTTATCGACTTTTGCTATGATAAAATTTTGAATCGGATGAGCAAATGACAACATACACTCGTAACACTCGTAACCTACCAGTTACTTACAAGTCAAGAAGTATGCATAAATTATGTTAGTTCTTTGTTCGAGAATATATTTCGTTTGGGAGTACATATCTTGAGCTTGATCTCCTAAATGAAGATTTTTTAGCTAAAATTTTGGAGTTCATTCAAGTAGGATTTCTGTTTTGTTGTTAATTACTCATTAATCTTGGTGGTTGCTGCTGGGTTTTTTCAGGCTGATATTATAATGGCCTTAGCTGGATCAAATGGGGGCTCTTGGTCAACAGCCTTAGCACCTAAATTAAATGTAACTGGTATATCTAGTGACCAGCAATCAGCTAAAGCATATCATGGTACGATAGCTACCGGTCGAGGAGTTGGCGATACTCTAATCTCGACACAAACAGGTGATTCTAACTTAAAGAAGTTCAGATTAACAGCAAAATATATTGGTATTGTTGGTCTTGGTTTTTGCAGCTATCAGGTTTTGAAGATGCAGAGTAAGATCTTATTTTGTTAGAAATTGCTTCCAAGCTTGATGATGTCAATCTGTATTATTAGGTTCATTTTAATGCCTTGTATGCATAATAAATTGACAAACCACCAACATTCTGGCTGTAGCTGTAGGCAATCGTCCACGCAGCGTTCAAGCGAAAGAGACTCGAGAACCCTCCGTTTGCATAGCAGACCCGAAGGCTGAACGGTAGAAAAAGAGAGGAGTTGAGTTGTAAGATTGCTGTTCCAATCTCATCATATCATATCATTTCATCGTCGTCGTCGTCGGTTTCTTGTTTTCTGAAAATAACAACAGTCAATTGCTCTCTAAGGCGACGATGGCTTATCTTTCCATCCCCATCCCGCCCGGGCCATTCTGGAGCAACCACCATTCTGGCTGCAGGTGATGATCATCACATCATGTAGCCAAAACTTCAAAAGAGGGTATAAAAGAGATTTATAGTACAAAAGTATTGTTGTGTCAAAGTCCAGTTTCATCAGCATTGTCATTATTATTATTATTATTGTCATTA

mRNA sequence

ATTTTCTTTCCAATTCAGAAAGCTGTCCTCATCCATCAAATTTTTCATCATTGATTTTTCTCTCAACAATTCCTTTCTCGATGTGATTTTGATTTTCATGAGCTCGAGGCCGGTTTGATTTTCGTTCGCGCGCAACCAAATTGACGGCCACGAACCAACGAATTCTGTGAATTTTTCCATTATTTAATCAAATCCGATCCGGTGAACGGAGGAAACTGAATCCCTCACCGGCGTCGGCGTCGGAAATTTTCTATAGAATCTTTTATCTCGAGAAAAGAGCCTGATTCTCTCTCTCTTCTGATTCTTTTGGCCTCTTTTTTCTGTATGTTTCTTTTCAAAAATCGTGTTGCTTGTCTTTTGTTTTTCTCTACACTCTTGCTCTTTATGCAGAGTGAAATGGATGCTTTCGTAAAGAGACAACTCTTGAGGTTCCATCTCTAATGGCTCATTCCAATGCGGGCTACTGCAGCAATGGCGAGAAGAAGAGCACGAGGCAGGTTCAACCACTAGGTAATGAGATCTTCCATGATTTTCTTGGCATTAGGACCAAGGATGCTTCTGTACTCTCGGCCGCGAAGACGCCGGATATAACCTTATCGGAAGCTTCGTCGCCGTCGTCGGCCTTGGCTTCTTCTAGCGGCGGCCGTGGACTCCTCTCCGCCGCTTCCGATCTTGCTTCTGAAAATCAGGTGGGAGGTTATCTTGAAGGGGTTCCCTTCTGCAGTCCAAGGAATGAGAAATCTGGACCTGAAAAAAGTAATTGGATAGCAGGGATTAAACGCAGTAATCCAGATCCTGGTTTTGTTGGATCATATAGAAACCAAATTCCTCATGTGCCTTCTGACTCTCTTGAGAGGTCACATACAATGAAGTCACTACAAACTGGACCTGGTGGAGATCGACCGAGATACAATGATAACGAGGCAGTTTACTACAGTATGAAGCCTCCAAAAATTGCTTCTTATTCCCTCACACAACACTCTCTTGGCACTAGATTTAATCCTAGTGTTACCAAATGGGAGAGGCCTATTCCATTGAACACGAGCTCGGCACAGAATTCTCCGCTCGGGATTCAGTTGATGCCTCGTGTACATCAAGTAGGTTCCAACTCTTCCAGAGAATTTAATGTTGCTCCTTCCAGTGTGTCTCAATCTGCTGCTGATGAAGGATCTAGAACTGGGATGAAAAGCCCTGGACTTTTGAGTAGCATTAATGTTGAAAATGATGGGAGACATTCTTCTCAAATGTTGCTAAGTTGTGACAAGCAGAAATCCAAGACTGAAGGTTTAGAATACAAATCTTCTAATCCTCCAAGTCAGCAGGGACTTGATTCGAACAACACGAAGATGACTATTTTCTATGGTGGTCAAGCTCATGTTTTTGATGATGTCCCTCCAAACAAGGCTGATATTATAATGGCCTTAGCTGGATCAAATGGGGGCTCTTGGTCAACAGCCTTAGCACCTAAATTAAATGTAACTGGTATATCTAGTGACCAGCAATCAGCTAAAGCATATCATGGTACGATAGCTACCGGTCGAGGAGTTGGCGATACTCTAATCTCGACACAAACAGCTGTAGGCAATCGTCCACGCAGCGTTCAAGCGAAAGAGACTCGAGAACCCTCCGTTTGCATAGCAGACCCGAAGGCTGAACGGTAGAAAAAGAGAGGAGTTGAGTTGTAAGATTGCTGTTCCAATCTCATCATATCATATCATTTCATCGTCGTCGTCGTCGGTTTCTTGTTTTCTGAAAATAACAACAGTCAATTGCTCTCTAAGGCGACGATGGCTTATCTTTCCATCCCCATCCCGCCCGGGCCATTCTGGAGCAACCACCATTCTGGCTGCAGGTGATGATCATCACATCATGTAGCCAAAACTTCAAAAGAGGGTATAAAAGAGATTTATAGTACAAAAGTATTGTTGTGTCAAAGTCCAGTTTCATCAGCATTGTCATTATTATTATTATTATTGTCATTA

Coding sequence (CDS)

ATGGCTCATTCCAATGCGGGCTACTGCAGCAATGGCGAGAAGAAGAGCACGAGGCAGGTTCAACCACTAGGTAATGAGATCTTCCATGATTTTCTTGGCATTAGGACCAAGGATGCTTCTGTACTCTCGGCCGCGAAGACGCCGGATATAACCTTATCGGAAGCTTCGTCGCCGTCGTCGGCCTTGGCTTCTTCTAGCGGCGGCCGTGGACTCCTCTCCGCCGCTTCCGATCTTGCTTCTGAAAATCAGGTGGGAGGTTATCTTGAAGGGGTTCCCTTCTGCAGTCCAAGGAATGAGAAATCTGGACCTGAAAAAAGTAATTGGATAGCAGGGATTAAACGCAGTAATCCAGATCCTGGTTTTGTTGGATCATATAGAAACCAAATTCCTCATGTGCCTTCTGACTCTCTTGAGAGGTCACATACAATGAAGTCACTACAAACTGGACCTGGTGGAGATCGACCGAGATACAATGATAACGAGGCAGTTTACTACAGTATGAAGCCTCCAAAAATTGCTTCTTATTCCCTCACACAACACTCTCTTGGCACTAGATTTAATCCTAGTGTTACCAAATGGGAGAGGCCTATTCCATTGAACACGAGCTCGGCACAGAATTCTCCGCTCGGGATTCAGTTGATGCCTCGTGTACATCAAGTAGGTTCCAACTCTTCCAGAGAATTTAATGTTGCTCCTTCCAGTGTGTCTCAATCTGCTGCTGATGAAGGATCTAGAACTGGGATGAAAAGCCCTGGACTTTTGAGTAGCATTAATGTTGAAAATGATGGGAGACATTCTTCTCAAATGTTGCTAAGTTGTGACAAGCAGAAATCCAAGACTGAAGGTTTAGAATACAAATCTTCTAATCCTCCAAGTCAGCAGGGACTTGATTCGAACAACACGAAGATGACTATTTTCTATGGTGGTCAAGCTCATGTTTTTGATGATGTCCCTCCAAACAAGGCTGATATTATAATGGCCTTAGCTGGATCAAATGGGGGCTCTTGGTCAACAGCCTTAGCACCTAAATTAAATGTAACTGGTATATCTAGTGACCAGCAATCAGCTAAAGCATATCATGGTACGATAGCTACCGGTCGAGGAGTTGGCGATACTCTAATCTCGACACAAACAGCTGTAGGCAATCGTCCACGCAGCGTTCAAGCGAAAGAGACTCGAGAACCCTCCGTTTGCATAGCAGACCCGAAGGCTGAACGGTAG

Protein sequence

MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSSALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPGFVGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER
Homology
BLAST of Carg00473 vs. NCBI nr
Match: KAG7031814.1 (Protein TIFY 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 797.3 bits (2058), Expect = 5.9e-227
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS
Sbjct: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQH 180
           FVGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQH
Sbjct: 121 FVGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQH 180

Query: 181 SLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAA 240
           SLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAA
Sbjct: 181 SLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAA 240

Query: 241 DEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNN 300
           DEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNN
Sbjct: 241 DEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNN 300

Query: 301 TKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYH 360
           TKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYH
Sbjct: 301 TKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYH 360

Query: 361 GTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 407
           GTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER
Sbjct: 361 GTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 406

BLAST of Carg00473 vs. NCBI nr
Match: KAG6608174.1 (Protein TIFY 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 784.6 bits (2025), Expect = 4.0e-223
Identity = 399/406 (98.28%), Postives = 402/406 (99.01%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAHSNAGYCSNGEKKSTRQVQPLGN IFHDFLGIRTKDASVLSAAKTPD+TLSEASSPSS
Sbjct: 1   MAHSNAGYCSNGEKKSTRQVQPLGNAIFHDFLGIRTKDASVLSAAKTPDVTLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPD G
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDSG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQH 180
           FVGSYRNQIPHVPSDSL+RSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQH
Sbjct: 121 FVGSYRNQIPHVPSDSLDRSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQH 180

Query: 181 SLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAA 240
           SLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAA
Sbjct: 181 SLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAA 240

Query: 241 DEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNN 300
           DEGSRTGMKSPGLLSSINV NDGRHSSQMLL+CDKQKSKTEGLEYKSSNPPSQQGLDSNN
Sbjct: 241 DEGSRTGMKSPGLLSSINVGNDGRHSSQMLLNCDKQKSKTEGLEYKSSNPPSQQGLDSNN 300

Query: 301 TKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYH 360
           TKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNV GISSDQQSAKAYH
Sbjct: 301 TKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVAGISSDQQSAKAYH 360

Query: 361 GTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 407
           GTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER
Sbjct: 361 GTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 406

BLAST of Carg00473 vs. NCBI nr
Match: XP_022940390.1 (uncharacterized protein LOC111446013 [Cucurbita moschata] >XP_022940391.1 uncharacterized protein LOC111446013 [Cucurbita moschata])

HSP 1 Score: 775.4 bits (2001), Expect = 2.4e-220
Identity = 401/423 (94.80%), Postives = 402/423 (95.04%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAH NAGYCSNGEKKSTRQVQPLGN IFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS
Sbjct: 1   MAHFNAGYCSNGEKKSTRQVQPLGNAIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMK-----------------SLQTGPGGDRPRYNDNEAV 180
           FVGSYRNQIPHVPSDSLERSHTMK                 SLQTGPGGDRPRYNDNEAV
Sbjct: 121 FVGSYRNQIPHVPSDSLERSHTMKMLQTGPGGDRLRYNDNESLQTGPGGDRPRYNDNEAV 180

Query: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240
           YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN
Sbjct: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240

Query: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGL 300
           SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINV NDGRHSSQMLLSCDKQKSKTEGL
Sbjct: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVGNDGRHSSQMLLSCDKQKSKTEGL 300

Query: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360
           EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK
Sbjct: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360

Query: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPK 407
           LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREP VCIA+PK
Sbjct: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPPVCIANPK 420

BLAST of Carg00473 vs. NCBI nr
Match: XP_023524217.1 (uncharacterized protein LOC111788190 [Cucurbita pepo subsp. pepo] >XP_023524218.1 uncharacterized protein LOC111788190 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766.5 bits (1978), Expect = 1.1e-217
Identity = 395/423 (93.38%), Postives = 399/423 (94.33%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAHSNAGYCSNGEKKSTRQVQPLGN IFHDFLGIRTKDASVLSAAKTPD+TLSEASSPSS
Sbjct: 1   MAHSNAGYCSNGEKKSTRQVQPLGNAIFHDFLGIRTKDASVLSAAKTPDVTLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPD G
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDSG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMK-----------------SLQTGPGGDRPRYNDNEAV 180
           FVGSYRNQIPHVPS+SLERSHTMK                 SLQTGPGGDRPRYNDNEAV
Sbjct: 121 FVGSYRNQIPHVPSESLERSHTMKMLQTGPGGDRPRYNDNESLQTGPGGDRPRYNDNEAV 180

Query: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240
           YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN
Sbjct: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240

Query: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGL 300
           SSREFNV PSSVSQSAADEGSRTGMKSPGLLSSINV NDGRHSSQMLLSCDKQKSKT GL
Sbjct: 241 SSREFNVGPSSVSQSAADEGSRTGMKSPGLLSSINVGNDGRHSSQMLLSCDKQKSKTGGL 300

Query: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360
           EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK
Sbjct: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360

Query: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPK 407
           LNVTGISSDQQSAKAYHGTIATGRGVGDTL+STQTAVGNRPRSVQAKETREP VC ADPK
Sbjct: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLVSTQTAVGNRPRSVQAKETREPPVCTADPK 420

BLAST of Carg00473 vs. NCBI nr
Match: XP_022981322.1 (protein TIFY 8-like [Cucurbita maxima] >XP_022981323.1 protein TIFY 8-like [Cucurbita maxima])

HSP 1 Score: 749.6 bits (1934), Expect = 1.4e-212
Identity = 388/423 (91.73%), Postives = 394/423 (93.14%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAHSNAGYCSNGE KSTRQVQPLGN IFHDFLGIRTKDAS+LSAAKTPD++LSEASSPSS
Sbjct: 1   MAHSNAGYCSNGENKSTRQVQPLGNAIFHDFLGIRTKDASILSAAKTPDVSLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKS+WIAGIKRSNPD G
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSSWIAGIKRSNPDSG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMK-----------------SLQTGPGGDRPRYNDNEAV 180
           FVGSYRNQIPHVPSDSLERSHTMK                 +LQTGPGGDRPRYNDNEAV
Sbjct: 121 FVGSYRNQIPHVPSDSLERSHTMKMLQTGPGGDRSRYNDNETLQTGPGGDRPRYNDNEAV 180

Query: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240
           YYSMKPPK ASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGI LMPRVHQVGS+
Sbjct: 181 YYSMKPPKFASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIPLMPRVHQVGSS 240

Query: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGL 300
           SSREFNVAPSSVSQ AADEGSRTGMKSPGLLSSINV NDGRHSSQMLLSCDKQKSKTEGL
Sbjct: 241 SSREFNVAPSSVSQPAADEGSRTGMKSPGLLSSINVGNDGRHSSQMLLSCDKQKSKTEGL 300

Query: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360
           EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK
Sbjct: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360

Query: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPK 407
           LNVTGISSDQQSAKAYHGTI TGRGVGDTLISTQT VGNRPRSVQ KETREP VC ADPK
Sbjct: 361 LNVTGISSDQQSAKAYHGTIPTGRGVGDTLISTQTDVGNRPRSVQVKETREP-VCTADPK 420

BLAST of Carg00473 vs. ExPASy Swiss-Prot
Match: Q84MB2 (Protein TIFY 8 OS=Arabidopsis thaliana OX=3702 GN=TIFY8 PE=1 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 8.2e-38
Identity = 121/331 (36.56%), Postives = 167/331 (50.45%), Query Frame = 0

Query: 27  IFHDFLGIRTKDASVLSAAK---TPDITLSEAS-SPSSALASSSGGRGLLSAASDLASEN 86
           +FHDFLG +    +  S A     PD   ++A+ +PS+A ASS+GG G LS+ SDL   +
Sbjct: 27  LFHDFLGSKNPTLASTSMADHRLPPDNKAAKAAMTPSTASASSAGGLGGLSSTSDLVERH 86

Query: 87  QVGG-YLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPGFVGSYRNQIPHVPSDSLERSH 146
             GG +L+G+    PR++ SG   SN  +G KRSN D  F             +  E  H
Sbjct: 87  SGGGNHLDGIQLFGPRSDVSGSIMSNRFSGNKRSNSDSHF----------TTQEHPETLH 146

Query: 147 TMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNT 206
             K L+ GPG                      S+S+                     +N 
Sbjct: 147 WSKLLRNGPG----------------------SFSMN--------------------VNP 206

Query: 207 SSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVEN 266
            + Q    G Q+   +HQ+ ++  ++ NV PS ++Q+AADEGSRTGMK PG+LSS  + N
Sbjct: 207 LANQPPRGGGQISHLLHQLSTSRFKDENVGPSVIAQTAADEGSRTGMKGPGILSSFTMPN 266

Query: 267 DGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNK 326
             +  S                 +  SN  +++ L S+  +MTIFYGGQAHVFDDV PNK
Sbjct: 267 SSKLES-----------------FAPSNTGNRKDLASSTKQMTIFYGGQAHVFDDVHPNK 288

Query: 327 ADIIMALAGSNGGSWSTALAPKLNVTGISSD 353
           AD+IMALAGS+GGSWST L+ K      +SD
Sbjct: 327 ADVIMALAGSSGGSWSTGLSHKPKSKNNTSD 288

BLAST of Carg00473 vs. ExPASy TrEMBL
Match: A0A6J1FQF4 (uncharacterized protein LOC111446013 OS=Cucurbita moschata OX=3662 GN=LOC111446013 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.2e-220
Identity = 401/423 (94.80%), Postives = 402/423 (95.04%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAH NAGYCSNGEKKSTRQVQPLGN IFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS
Sbjct: 1   MAHFNAGYCSNGEKKSTRQVQPLGNAIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMK-----------------SLQTGPGGDRPRYNDNEAV 180
           FVGSYRNQIPHVPSDSLERSHTMK                 SLQTGPGGDRPRYNDNEAV
Sbjct: 121 FVGSYRNQIPHVPSDSLERSHTMKMLQTGPGGDRLRYNDNESLQTGPGGDRPRYNDNEAV 180

Query: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240
           YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN
Sbjct: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240

Query: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGL 300
           SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINV NDGRHSSQMLLSCDKQKSKTEGL
Sbjct: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVGNDGRHSSQMLLSCDKQKSKTEGL 300

Query: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360
           EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK
Sbjct: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360

Query: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPK 407
           LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREP VCIA+PK
Sbjct: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPPVCIANPK 420

BLAST of Carg00473 vs. ExPASy TrEMBL
Match: A0A6J1ITN4 (protein TIFY 8-like OS=Cucurbita maxima OX=3661 GN=LOC111480486 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 6.9e-213
Identity = 388/423 (91.73%), Postives = 394/423 (93.14%), Query Frame = 0

Query: 1   MAHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSS 60
           MAHSNAGYCSNGE KSTRQVQPLGN IFHDFLGIRTKDAS+LSAAKTPD++LSEASSPSS
Sbjct: 1   MAHSNAGYCSNGENKSTRQVQPLGNAIFHDFLGIRTKDASILSAAKTPDVSLSEASSPSS 60

Query: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPG 120
           ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKS+WIAGIKRSNPD G
Sbjct: 61  ALASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSSWIAGIKRSNPDSG 120

Query: 121 FVGSYRNQIPHVPSDSLERSHTMK-----------------SLQTGPGGDRPRYNDNEAV 180
           FVGSYRNQIPHVPSDSLERSHTMK                 +LQTGPGGDRPRYNDNEAV
Sbjct: 121 FVGSYRNQIPHVPSDSLERSHTMKMLQTGPGGDRSRYNDNETLQTGPGGDRPRYNDNEAV 180

Query: 181 YYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSN 240
           YYSMKPPK ASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGI LMPRVHQVGS+
Sbjct: 181 YYSMKPPKFASYSLTQHSLGTRFNPSVTKWERPIPLNTSSAQNSPLGIPLMPRVHQVGSS 240

Query: 241 SSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGL 300
           SSREFNVAPSSVSQ AADEGSRTGMKSPGLLSSINV NDGRHSSQMLLSCDKQKSKTEGL
Sbjct: 241 SSREFNVAPSSVSQPAADEGSRTGMKSPGLLSSINVGNDGRHSSQMLLSCDKQKSKTEGL 300

Query: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360
           EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK
Sbjct: 301 EYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPK 360

Query: 361 LNVTGISSDQQSAKAYHGTIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPK 407
           LNVTGISSDQQSAKAYHGTI TGRGVGDTLISTQT VGNRPRSVQ KETREP VC ADPK
Sbjct: 361 LNVTGISSDQQSAKAYHGTIPTGRGVGDTLISTQTDVGNRPRSVQVKETREP-VCTADPK 420

BLAST of Carg00473 vs. ExPASy TrEMBL
Match: A0A5D3E7X9 (Protein TIFY 8-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G007240 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.1e-157
Identity = 306/410 (74.63%), Postives = 335/410 (81.71%), Query Frame = 0

Query: 2   AHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSSA 61
           A+S+AGYCSNGEKKST QV  LGN IFHDFLGI+TKDASVL AAKTPD++ SEAS+P SA
Sbjct: 6   AYSDAGYCSNGEKKSTSQVPQLGNAIFHDFLGIKTKDASVLLAAKTPDVSFSEASTPPSA 65

Query: 62  LASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPGF 121
           LASSSGGRGL+SAASDLASENQV  YLEGVPF SPRN+ SGPE  N IAGIKR NPD  F
Sbjct: 66  LASSSGGRGLISAASDLASENQVCDYLEGVPFYSPRNDISGPENGNRIAGIKRGNPDSAF 125

Query: 122 VGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQHS 181
           +G YRNQIPH+             LQ G GGDRPRYNDNE V YSM+PP+IAS SLTQHS
Sbjct: 126 MGPYRNQIPHI-------------LQNGAGGDRPRYNDNEVV-YSMQPPRIASSSLTQHS 185

Query: 182 LGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAAD 241
           LGTRFNPSV+KWERPIP N SSA NS LG Q +PRVHQ+ SNSSREFNVAPS++SQSAAD
Sbjct: 186 LGTRFNPSVSKWERPIPSNMSSALNSSLGSQFVPRVHQIASNSSREFNVAPSNISQSAAD 245

Query: 242 EGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNNT 301
           EGSRTGMKSPGLLSS N  +D RHSSQMLLSCDKQK+KTE L YKSSN  S QGLDS+N 
Sbjct: 246 EGSRTGMKSPGLLSSFNARHDRRHSSQMLLSCDKQKTKTESLAYKSSNSSSPQGLDSDNR 305

Query: 302 KMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYHG 361
           +MTIFYGGQAHVFDDV PNKADIIMALAGSNGGSWST LAPKLNVT + S+Q+SAK YHG
Sbjct: 306 QMTIFYGGQAHVFDDVHPNKADIIMALAGSNGGSWSTNLAPKLNVTRVPSEQESAKDYHG 365

Query: 362 -----TIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 407
                 I+T RGVGDT I TQTAVG+ PRSV  +ETR+P V  AD  AE+
Sbjct: 366 KLYHSLISTSRGVGDTPIPTQTAVGDHPRSVAVEETRKP-VRAADINAEQ 400

BLAST of Carg00473 vs. ExPASy TrEMBL
Match: A0A0A0LJC4 (Tify domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G878900 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.1e-157
Identity = 305/410 (74.39%), Postives = 337/410 (82.20%), Query Frame = 0

Query: 2   AHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSSA 61
           A+S+AGYCSNGEKKST QV  LGN IFHDFLGI+TKDASVL AAKTPD++ SEAS+P SA
Sbjct: 6   AYSDAGYCSNGEKKSTSQVPQLGNAIFHDFLGIKTKDASVLLAAKTPDVSFSEASTPPSA 65

Query: 62  LASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPGF 121
           LASSSGGRGL+SAASDLASENQV  YLEGVPF SPRN+ SGPE  N IAGIKR NPD  F
Sbjct: 66  LASSSGGRGLISAASDLASENQVCDYLEGVPFYSPRNDISGPENGNRIAGIKRGNPDSAF 125

Query: 122 VGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQHS 181
           +G YRNQIPH+             LQ G GGDRPRYNDNE V YSM+PP+IAS SLTQHS
Sbjct: 126 MGPYRNQIPHI-------------LQNGAGGDRPRYNDNEVV-YSMQPPRIASSSLTQHS 185

Query: 182 LGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAAD 241
           LGTRFNPSV+KWERPIP N SSA NS LG Q +PRVHQ+ SNSSREFNVAPS++SQSAAD
Sbjct: 186 LGTRFNPSVSKWERPIPSNMSSALNSSLGSQFVPRVHQIASNSSREFNVAPSNISQSAAD 245

Query: 242 EGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNNT 301
           EGSRTGMK+PGLLSS N  +D RHSSQML SCDKQK+KTE L YKSSNP SQQGLDS+N 
Sbjct: 246 EGSRTGMKNPGLLSSFNARHDQRHSSQMLQSCDKQKTKTESLAYKSSNPSSQQGLDSDNR 305

Query: 302 KMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYHG 361
           +MTIFYGGQAHVFDDV PNKADIIMALAGSNGGSWST LAPKLN+T I S+Q+SAK YHG
Sbjct: 306 QMTIFYGGQAHVFDDVHPNKADIIMALAGSNGGSWSTNLAPKLNITRIPSEQESAKDYHG 365

Query: 362 -----TIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 407
                 I+T RGVGDT ISTQ AVG+ PRSV A+ET++ +V  AD  AE+
Sbjct: 366 KLYHSLISTSRGVGDTPISTQAAVGDHPRSVAAEETQK-AVRAADLNAEQ 400

BLAST of Carg00473 vs. ExPASy TrEMBL
Match: A0A1S3CR31 (protein TIFY 8-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503802 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 1.1e-157
Identity = 306/410 (74.63%), Postives = 335/410 (81.71%), Query Frame = 0

Query: 2   AHSNAGYCSNGEKKSTRQVQPLGNEIFHDFLGIRTKDASVLSAAKTPDITLSEASSPSSA 61
           A+S+AGYCSNGEKKST QV  LGN IFHDFLGI+TKDASVL AAKTPD++ SEAS+P SA
Sbjct: 6   AYSDAGYCSNGEKKSTSQVPQLGNAIFHDFLGIKTKDASVLLAAKTPDVSFSEASTPPSA 65

Query: 62  LASSSGGRGLLSAASDLASENQVGGYLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPGF 121
           LASSSGGRGL+SAASDLASENQV  YLEGVPF SPRN+ SGPE  N IAGIKR NPD  F
Sbjct: 66  LASSSGGRGLISAASDLASENQVCDYLEGVPFYSPRNDISGPENGNRIAGIKRGNPDSAF 125

Query: 122 VGSYRNQIPHVPSDSLERSHTMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQHS 181
           +G YRNQIPH+             LQ G GGDRPRYNDNE V YSM+PP+IAS SLTQHS
Sbjct: 126 MGPYRNQIPHI-------------LQNGAGGDRPRYNDNEVV-YSMQPPRIASSSLTQHS 185

Query: 182 LGTRFNPSVTKWERPIPLNTSSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAAD 241
           LGTRFNPSV+KWERPIP N SSA NS LG Q +PRVHQ+ SNSSREFNVAPS++SQSAAD
Sbjct: 186 LGTRFNPSVSKWERPIPSNMSSALNSSLGSQFVPRVHQIASNSSREFNVAPSNISQSAAD 245

Query: 242 EGSRTGMKSPGLLSSINVENDGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNNT 301
           EGSRTGMKSPGLLSS N  +D RHSSQMLLSCDKQK+KTE L YKSSN  S QGLDS+N 
Sbjct: 246 EGSRTGMKSPGLLSSFNARHDRRHSSQMLLSCDKQKTKTESLAYKSSNSSSPQGLDSDNR 305

Query: 302 KMTIFYGGQAHVFDDVPPNKADIIMALAGSNGGSWSTALAPKLNVTGISSDQQSAKAYHG 361
           +MTIFYGGQAHVFDDV PNKADIIMALAGSNGGSWST LAPKLNVT + S+Q+SAK YHG
Sbjct: 306 QMTIFYGGQAHVFDDVHPNKADIIMALAGSNGGSWSTNLAPKLNVTRVPSEQESAKDYHG 365

Query: 362 -----TIATGRGVGDTLISTQTAVGNRPRSVQAKETREPSVCIADPKAER 407
                 I+T RGVGDT I TQTAVG+ PRSV  +ETR+P V  AD  AE+
Sbjct: 366 KLYHSLISTSRGVGDTPIPTQTAVGDHPRSVAVEETRKP-VRAADINAEQ 400

BLAST of Carg00473 vs. TAIR 10
Match: AT4G32570.1 (TIFY domain protein 8 )

HSP 1 Score: 159.5 bits (402), Expect = 5.9e-39
Identity = 121/331 (36.56%), Postives = 167/331 (50.45%), Query Frame = 0

Query: 27  IFHDFLGIRTKDASVLSAAK---TPDITLSEAS-SPSSALASSSGGRGLLSAASDLASEN 86
           +FHDFLG +    +  S A     PD   ++A+ +PS+A ASS+GG G LS+ SDL   +
Sbjct: 27  LFHDFLGSKNPTLASTSMADHRLPPDNKAAKAAMTPSTASASSAGGLGGLSSTSDLVERH 86

Query: 87  QVGG-YLEGVPFCSPRNEKSGPEKSNWIAGIKRSNPDPGFVGSYRNQIPHVPSDSLERSH 146
             GG +L+G+    PR++ SG   SN  +G KRSN D  F             +  E  H
Sbjct: 87  SGGGNHLDGIQLFGPRSDVSGSIMSNRFSGNKRSNSDSHF----------TTQEHPETLH 146

Query: 147 TMKSLQTGPGGDRPRYNDNEAVYYSMKPPKIASYSLTQHSLGTRFNPSVTKWERPIPLNT 206
             K L+ GPG                      S+S+                     +N 
Sbjct: 147 WSKLLRNGPG----------------------SFSMN--------------------VNP 206

Query: 207 SSAQNSPLGIQLMPRVHQVGSNSSREFNVAPSSVSQSAADEGSRTGMKSPGLLSSINVEN 266
            + Q    G Q+   +HQ+ ++  ++ NV PS ++Q+AADEGSRTGMK PG+LSS  + N
Sbjct: 207 LANQPPRGGGQISHLLHQLSTSRFKDENVGPSVIAQTAADEGSRTGMKGPGILSSFTMPN 266

Query: 267 DGRHSSQMLLSCDKQKSKTEGLEYKSSNPPSQQGLDSNNTKMTIFYGGQAHVFDDVPPNK 326
             +  S                 +  SN  +++ L S+  +MTIFYGGQAHVFDDV PNK
Sbjct: 267 SSKLES-----------------FAPSNTGNRKDLASSTKQMTIFYGGQAHVFDDVHPNK 288

Query: 327 ADIIMALAGSNGGSWSTALAPKLNVTGISSD 353
           AD+IMALAGS+GGSWST L+ K      +SD
Sbjct: 327 ADVIMALAGSSGGSWSTGLSHKPKSKNNTSD 288

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031814.15.9e-227100.00Protein TIFY 8 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6608174.14.0e-22398.28Protein TIFY 8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022940390.12.4e-22094.80uncharacterized protein LOC111446013 [Cucurbita moschata] >XP_022940391.1 unchar... [more]
XP_023524217.11.1e-21793.38uncharacterized protein LOC111788190 [Cucurbita pepo subsp. pepo] >XP_023524218.... [more]
XP_022981322.11.4e-21291.73protein TIFY 8-like [Cucurbita maxima] >XP_022981323.1 protein TIFY 8-like [Cucu... [more]
Match NameE-valueIdentityDescription
Q84MB28.2e-3836.56Protein TIFY 8 OS=Arabidopsis thaliana OX=3702 GN=TIFY8 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FQF41.2e-22094.80uncharacterized protein LOC111446013 OS=Cucurbita moschata OX=3662 GN=LOC1114460... [more]
A0A6J1ITN46.9e-21391.73protein TIFY 8-like OS=Cucurbita maxima OX=3661 GN=LOC111480486 PE=3 SV=1[more]
A0A5D3E7X91.1e-15774.63Protein TIFY 8-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A0A0LJC41.1e-15774.39Tify domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G878900 PE=3 S... [more]
A0A1S3CR311.1e-15774.63protein TIFY 8-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503802 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G32570.15.9e-3936.56TIFY domain protein 8 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010399Tify domainSMARTSM00979tify_2coord: 296..331
e-value: 7.1E-14
score: 62.1
IPR010399Tify domainPFAMPF06200tifycoord: 299..330
e-value: 2.9E-14
score: 52.1
IPR010399Tify domainPROSITEPS51320TIFYcoord: 296..331
score: 14.97526
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..299
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..275
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 379..406
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..71
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 283..299
NoneNo IPR availablePANTHERPTHR33077:SF8PROTEIN TIFY 8coord: 66..403
IPR040390TIFY/JAZ familyPANTHERPTHR33077PROTEIN TIFY 4A-RELATED-RELATEDcoord: 66..403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00473-RACarg00473-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031347 regulation of defense response
biological_process GO:2000022 regulation of jasmonic acid mediated signaling pathway
biological_process GO:0009611 response to wounding
cellular_component GO:0005634 nucleus