Carg00271 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00271
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDolichyl-phosphate beta-glucosyltransferase
LocationCarg_Chr04: 6306410 .. 6310923 (-)
RNA-Seq ExpressionCarg00271
SyntenyCarg00271
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCATATTATTCATTTAGCAACACGGTACCGGAGGCACCAAACGACGCCTTTGGGAGGAGGGGAACTCTGAGAGCAACGATGGCGTCTCTGTGGTCTTTGATGGAGGTTCTCGTCTATCTCTTTCTCGTTGCAGCTTGTGGATTACTCTCCGCTATTATATTCGAAGCTAACAGAAGAAGGCTTAACCACTCGTACGTTCAATTTCCACTTCCTCATCCTTAAATCTCATCTGGTTTACGATTCCGCATTTTGATTTCTTTCTCCCGTTTCCTTGCATTCATCTGATCTTCCGTCGTTCAATTTCTCGATTCTGTATCTGATTGCATCTTTTCTCCATCGGAATCTTAATAGTCACGTTGAAGCTCCTGCGGTCTTTGAGGATCCCAATACGTCGACGCAGGTATTTCCAGTCTCTGATACCAATTATTAGTGATTCGATCGCATGTTTTGTTAACGTTCTGTATGTATCATTCGAGATCTACTGCAGGAAGTTGATAATGTATGCGATTTGTTATGTTGTCAGTCTGATTGAAATCCGGAATGAGGTATTCTGGAATTGATATTTCTGTTGTTTTATGTAGGTTTCTTGTCCTTCCATCTTTGATCCGGCTGAGAAGTATATATCTTTGGTTATTCCAGCATATAATGAAGAGCAGAGGCTTCCCGGTGCTCTGCAAGAAACCATTGAGTAAGTTGAATGGGAACTGTGTGTGAATTTCACCCCCTCCCCCTCGAGCATTAGCTGATTTATGCCATTTGGATCATATTCATGAACCTTTTTTTCAGAGACGATTATGACATATCAAAAGAACAATCTCGTTTACTTATCAAGGCATATTTGAATCTCTCGGATTTCTTTTGCTTTTAGTTGGAAAGTTTCAAAAAAATTAATCAAAACTTCCAAATAATTGGAAGCTGCTTTACATTTTTAGTCTATACGTTGGCCATAATCGTCCGCACCAATATTGGTGCCTCAAATCTAGAAAGATTATTAACCATTTCTGAATAAAATTTATCTTCATAAATCAGTATCACATACTTGATCTCTTGTTGAATCTTCGCTAGACCTTAATCATATAATTTTTTAGTAGATAAGGTAGGCAATTTCTCAGAGAGGTGCACTTGGTTACTATTAGATTATTGTTCTTGAAGGACTTAAAAAAGGTTGCACAATTTATGCACAATATTATTGCCGTGTTTGTTTTGTAGTTATCTTCAAGAACGTGCAGCAAAGGATAAATCATTCTCATACGAGGTACAGATCATCTCTTAATCTTCTGCATTGATTTAATGTTGTGCATTGCTTCTTGAAGAATAAAATAATTGTTTGAATCTGAATCTTGTGGAACGTGTTGCTGATCTTATAAAACTATTAAAATTGTCTTTTCTAATTTTGTGCCTCGAGACAGTCTTTCCCCTTCCAGTTTGGTGAGAGCCCTTGTCAGGAATTGGGAGTGAGCTTCTTTCTGTTAGGTAGTTCACGTTCATGGTACTGGAAATAATTTGTTTTCCATGACAAAAACTATTTCCCATTTTTAGAACTATGGTCTAGTGCCTTTCTTGATATGTATGGGAAAAAATAATTAAATAATCTGTGGCTCATGTTAATGTCCTGTCTCCTGCCTGAATACCTTTCTCAAGTAAGAACAAACATCATGAGATCGATGTGTTTAATATGGCAGAATACATTTAACTTGTCTATGTATTAAGGCTTATTTCTTAGTATGTATTATTACGTTGATGAAATTTTGATAGAACTCTCTGATCTTTCTTTAAAAAGGCTATTGCTAAATATGAAACAAATAGTAAGCAAGTGACACTGAACCCTCCCTATCCAACTTCTTTAACAAATTGGCATTACTTTCCACTCCGGATCTCATTTTCGTTCAGTTAATTTCATCTCCACCCAATTCCCAAGGTGGCCAATGATGTCCTCTCACTCCTTCAGCAGTGCTTAGCTACCCGGTTCAATACCCAGTTGAATGTGGACCTCTCTCTCTCTCTCTCTCTCTTTTTTTTTTTTCCTTCCCATCATTCAGCTCAGTTAACTGGTTTTATTGGCTTCTTTCTCCTATTATAGAAGAATACCGAGTATTTATCATGCTCTTTCTAACCTAATGACAGAGTCATTTCAGAATGTAGAATATATTTGGTCTACTCTCCGGTTCTTTAATTTTGAAATTGATGAAAACTTGATCAGGTGGTCATTGTTGATGATGGAAGCATAGATGGGACAAAAAGAGTTGGTCGGGAATTTTCCAAGAAGTTCTCAGTTGACAATGTGAGGGTTATCCTTCTTGGAAGAAATCATGGAAAGGGAGAAGCGATTAGAAAAGTGAGTCCATTCTAAGCAGGTTATTTGTTATCAGTTTCTGATGTTACATTTTCTGGCAACAATCCATTGAAATCTCTAGTCTCTGCGGAAGCATTGTTAAACAGGGGATGTTGCATTCACGTGGTGAATTACTTTTAATGTTGGACGCTGATGGAGCAACCAAAGTTAATGACCTAGAAAAACTTGAAAATCAGGTCATCCTCATTCTTATTTTAAGCTGTTGATATTTTACTGGGCTGTTATTCTTAAAGTTTTGCCTTGGGCGTACAAGACTTCCAATATTAGCTATTGAGGACTACTTTATGACATTCCAGATCCATGCCTTTGCCAAAAAGGAATCTAGTGGTTCTAGTTTTAGAGTTTCTGATATCCCGGTTGCGGCTTTTGGTTCTCGTGCTCATTTGGAGGAAAAGGCTCTAGCTACAGTTAAGTACTTTATTTAGGCCAAATCAATGGAATAATTATTGTTCGTTGTACTGTTTCTCTTTCATTCATCTTAATGAAAACATGGTTTCTCATCCATATTCGTACAGCATGTACATAATGTTATTATTTTGAATTTGCTTAGGATTGTCTAGGCTGAGCCATCTAATCTGAACCGTTTCACTTATTTCTCTGAGACGGTGCATGTATATATAATGTTACTTTTTTGGAATTCGCAAAGGTTTGTCCAGAATGAGCCATCTCATCATTTCTCCTTGCTTTATTCTGAACTATTTCCCATATTTTTCAGAGAAAGATGTACCGTAATTTCTTGATGAAAGGTTTCCATCTTGTGGTTCTTTTAACTGCCGGTCCTGGAATACGTGATACCCAGGTGATTTGTGCTTCCCTACAGATATTAATCATTTACTGGTTTTAGTTTTCCTTGGATACCATATATTACAATAGATACTATTTTCTCAAGTGAAGATAATTGATTTTAGTTAATGATCTTCTCGGTGATAACTGATTGCTTCATGTTTTACAGTGTGGTTTCAAGATGTTTACAAGATCTGCAGCAAGAAAGCTTTTCACAAATATTCGTTTGAAAAGGTAGATGAATCCTGCCGACCTAATTTGTCCATGCTCCTCAATTTCAAAATCCAGATTCTAATAGCATTGCTGAACTGATATTGTTCTGATTTTGTGTCTGCTATTTATGATAACTTGGTAACTTTGAGATAGCATCCATCATTTCAGGGCTGTCTTCTCTCTCTTTTTGCGTTTTGTTATTTTGTTATTTTTTTTCCTTTTCGATGAGGAGGAGGTAAAGTGTTGTCCTTCTTGACTCTATTATTACAGAATCTTAGACCGTACATTTGAGCTTTGAAGGAAAAGACCTAAATGACGTAAAATTTTGGACGACAAAGCAAGCCATCACATTGACAGAAAAATAATGCTTATTTCTCCATTGCCCTTACATACTAATTTATTGTGGTCTCTGTCTAGACTGGTGTCTATGATGAAACTTCGATGCTCATTTTGCCTCCACTTGTTTCATTACTGTATGTGGTCATTGAATACTGTTAAGAAGACAAATTCAAGTCATTTGTCTGTGCACGCTTTATATTTTGCATATCTTGTTCATGGAACTGACTTTGTTGTGCACTTTTTACAGATGGTGCTTCGATGTCGAGATTGTTTATCTCTGCAAACGATTTCACATTCCGATGGTTGAAATATCTGTGAACTGGTCTGAAATCCCTGGATCGAAGGTAAATCCATTGAGCATTCCCAACATGCTTTGGGAGCTGGCTCTCATGTCTATGGGATATAGGACAGGTATGTGGAGAGTTCATACATAACCTTTTCGATTGTCCGATAAGGCAATAACCACAGTAATCTCGGCGATCAATGATTTCATTTAGGACCAAATACGAGCACACGAAATGTTAGTTGAGCAATATTTCATACTGTGTCAGCTTGCGTAGGCAAGCCACAGCCTGACCATTTCATTTATGTAGAATTATTGGGATGTTAAAAACTTTTACTCATACTTTCATACCGGCTTATGAATCTGATGTCAAAGTTCTAAAGAAATATTGATTTAATTTCCCAAGTTCTCAAATCCAATTTTGTTTGCTATATTTTATCTTTTTTATAATCTTCAGAACATGGGACAACCCAAAAATCTTCCCTAAAATTTTTTACGATGGAAATGAGAAATCCCTCTTTAGAGTTTTTACTTTTTATTTTA

mRNA sequence

GCATATTATTCATTTAGCAACACGGTACCGGAGGCACCAAACGACGCCTTTGGGAGGAGGGGAACTCTGAGAGCAACGATGGCGTCTCTGTGGTCTTTGATGGAGGTTCTCGTCTATCTCTTTCTCGTTGCAGCTTGTGGATTACTCTCCGCTATTATATTCGAAGCTAACAGAAGAAGGCTTAACCACTCTCACGTTGAAGCTCCTGCGGTCTTTGAGGATCCCAATACGTCGACGCAGGTTTCTTGTCCTTCCATCTTTGATCCGGCTGAGAAGTATATATCTTTGGTTATTCCAGCATATAATGAAGAGCAGAGGCTTCCCGGTGCTCTGCAAGAAACCATTGATTATCTTCAAGAACGTGCAGCAAAGGATAAATCATTCTCATACGAGGTGGTCATTGTTGATGATGGAAGCATAGATGGGACAAAAAGAGTTGGTCGGGAATTTTCCAAGAAGTTCTCAGTTGACAATGTGAGGGTTATCCTTCTTGGAAGAAATCATGGAAAGGGAGAAGCGATTAGAAAAGGGATGTTGCATTCACGTGGTGAATTACTTTTAATGTTGGACGCTGATGGAGCAACCAAAGTTAATGACCTAGAAAAACTTGAAAATCAGATCCATGCCTTTGCCAAAAAGGAATCTAGTGGTTCTAGTTTTAGAGTTTCTGATATCCCGGTTGCGGCTTTTGGTTCTCGTGCTCATTTGGAGGAAAAGGCTCTAGCTACAAGAAAGATGTACCGTAATTTCTTGATGAAAGGTTTCCATCTTGTGGTTCTTTTAACTGCCGGTCCTGGAATACGTGATACCCAGTGTGGTTTCAAGATGTTTACAAGATCTGCAGCAAGAAAGCTTTTCACAAATATTCGTTTGAAAAGATGGTGCTTCGATGTCGAGATTGTTTATCTCTGCAAACGATTTCACATTCCGATGGTTGAAATATCTGTGAACTGGTCTGAAATCCCTGGATCGAAGGTAAATCCATTGAGCATTCCCAACATGCTTTGGGAGCTGGCTCTCATGTCTATGGGATATAGGACAGGTATGTGGAGAGTTCATACATAACCTTTTCGATTGTCCGATAAGGCAATAACCACAGTAATCTCGGCGATCAATGATTTCATTTAGGACCAAATACGAGCACACGAAATGTTAGTTGAGCAATATTTCATACTGTGTCAGCTTGCGTAGGCAAGCCACAGCCTGACCATTTCATTTATGTAGAATTATTGGGATGTTAAAAACTTTTACTCATACTTTCATACCGGCTTATGAATCTGATGTCAAAGTTCTAAAGAAATATTGATTTAATTTCCCAAGTTCTCAAATCCAATTTTGTTTGCTATATTTTATCTTTTTTATAATCTTCAGAACATGGGACAACCCAAAAATCTTCCCTAAAATTTTTTACGATGGAAATGAGAAATCCCTCTTTAGAGTTTTTACTTTTTATTTTA

Coding sequence (CDS)

ATGGCGTCTCTGTGGTCTTTGATGGAGGTTCTCGTCTATCTCTTTCTCGTTGCAGCTTGTGGATTACTCTCCGCTATTATATTCGAAGCTAACAGAAGAAGGCTTAACCACTCTCACGTTGAAGCTCCTGCGGTCTTTGAGGATCCCAATACGTCGACGCAGGTTTCTTGTCCTTCCATCTTTGATCCGGCTGAGAAGTATATATCTTTGGTTATTCCAGCATATAATGAAGAGCAGAGGCTTCCCGGTGCTCTGCAAGAAACCATTGATTATCTTCAAGAACGTGCAGCAAAGGATAAATCATTCTCATACGAGGTGGTCATTGTTGATGATGGAAGCATAGATGGGACAAAAAGAGTTGGTCGGGAATTTTCCAAGAAGTTCTCAGTTGACAATGTGAGGGTTATCCTTCTTGGAAGAAATCATGGAAAGGGAGAAGCGATTAGAAAAGGGATGTTGCATTCACGTGGTGAATTACTTTTAATGTTGGACGCTGATGGAGCAACCAAAGTTAATGACCTAGAAAAACTTGAAAATCAGATCCATGCCTTTGCCAAAAAGGAATCTAGTGGTTCTAGTTTTAGAGTTTCTGATATCCCGGTTGCGGCTTTTGGTTCTCGTGCTCATTTGGAGGAAAAGGCTCTAGCTACAAGAAAGATGTACCGTAATTTCTTGATGAAAGGTTTCCATCTTGTGGTTCTTTTAACTGCCGGTCCTGGAATACGTGATACCCAGTGTGGTTTCAAGATGTTTACAAGATCTGCAGCAAGAAAGCTTTTCACAAATATTCGTTTGAAAAGATGGTGCTTCGATGTCGAGATTGTTTATCTCTGCAAACGATTTCACATTCCGATGGTTGAAATATCTGTGAACTGGTCTGAAATCCCTGGATCGAAGGTAAATCCATTGAGCATTCCCAACATGCTTTGGGAGCTGGCTCTCATGTCTATGGGATATAGGACAGGTATGTGGAGAGTTCATACATAA

Protein sequence

MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSIFDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRVGREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQIHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKVNPLSIPNMLWELALMSMGYRTGMWRVHT
Homology
BLAST of Carg00271 vs. NCBI nr
Match: KAG7031614.1 (Dolichyl-phosphate beta-glucosyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 654.1 bits (1686), Expect = 6.6e-184
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. NCBI nr
Match: KAG6601000.1 (Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 653.7 bits (1685), Expect = 8.6e-184
Identity = 327/328 (99.70%), Postives = 328/328 (100.00%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVL+YLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWSLMEVLIYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. NCBI nr
Match: XP_022990413.1 (dolichyl-phosphate beta-glucosyltransferase-like [Cucurbita maxima])

HSP 1 Score: 651.7 bits (1680), Expect = 3.3e-183
Identity = 326/328 (99.39%), Postives = 327/328 (99.70%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVL+YLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWSLMEVLIYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERA KDKSFSYEVVIVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAEKDKSFSYEVVIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. NCBI nr
Match: XP_023518326.1 (dolichyl-phosphate beta-glucosyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.4 bits (1679), Expect = 4.3e-183
Identity = 326/328 (99.39%), Postives = 327/328 (99.70%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVL+YLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNT TQVSCPSI
Sbjct: 1   MASLWSLMEVLIYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTLTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. NCBI nr
Match: XP_022957336.1 (dolichyl-phosphate beta-glucosyltransferase-like [Cucurbita moschata])

HSP 1 Score: 646.4 bits (1666), Expect = 1.4e-181
Identity = 324/328 (98.78%), Postives = 327/328 (99.70%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVL+YLFLVAACGLLSAIIFEANRRRLN+S+VEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWSLMEVLIYLFLVAACGLLSAIIFEANRRRLNYSYVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTK V
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKGV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. ExPASy Swiss-Prot
Match: Q9VLQ1 (Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=wol PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 1.7e-65
Identity = 142/327 (43.43%), Postives = 198/327 (60.55%), Query Frame = 0

Query: 4   LWS-LMEVLVYLFLVAACGLLS--AIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 63
           +W+ L ++  YL    A   LS  A++    +   N    +    F DP+T   V+ PS+
Sbjct: 1   MWTCLCQLCFYLLSTLAVAALSIAALVLYKTKPYPNIKRHKDEETFLDPHTIKTVTFPSL 60

Query: 64  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 123
            D     +S+++PAYNEEQRLP  L E + +L++++A   +F+YEV++V DGS D T  V
Sbjct: 61  EDSPSLELSVIVPAYNEEQRLPSMLDECLAFLEQKSAGTPNFTYEVIVVSDGSQDATVSV 120

Query: 124 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 183
              +SKK   + VRV+ L  N GKG A+R GML +RG  LL  DADGATK  D +KLE  
Sbjct: 121 ALGYSKKHGAEKVRVLELIENRGKGGAVRMGMLSARGRNLLFADADGATKFPDYDKLEVA 180

Query: 184 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 243
           +   A +      +R   I   A GSRAHLE  A+ATR  +R  LM GFH +V L A   
Sbjct: 181 LKQLAPE------WRDDGI---AIGSRAHLENDAIATRSFFRTILMHGFHFLVWLFAVRS 240

Query: 244 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 303
           IRDTQCGFK+FTR+ ARKLFT++ ++RW FDVE++YL +   +PM E++V W+EI GSK+
Sbjct: 241 IRDTQCGFKLFTRTTARKLFTSLHVERWAFDVELLYLAENLKLPMSEVAVRWTEIDGSKL 300

Query: 304 NPL-SIPNMLWELALMSMGYRTGMWRV 327
            P  S   M  +L ++ + Y  G WR+
Sbjct: 301 TPFWSWLQMGRDLFMIWVRYLVGAWRI 318

BLAST of Carg00271 vs. ExPASy Swiss-Prot
Match: Q9Y673 (Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.2e-65
Identity = 142/328 (43.29%), Postives = 200/328 (60.98%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIF-EANRRRLNHSHVEAPAVFEDPNTSTQVSCPS 60
           MA L   + VL      AA  L+S + F  A +    H H E    F       + + PS
Sbjct: 1   MAPLLLQLAVLGAALAAAALVLISIVAFTTATKMPALHRHEEEK--FFLNAKGQKETLPS 60

Query: 61  IFDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKR 120
           I+D   K +S+V+P+YNEE+RLP  + E + YL++R  +D +F+YEV++VDDGS D T +
Sbjct: 61  IWDSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSK 120

Query: 121 VGREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLEN 180
           V  ++ +K+  D VRVI L +N GKG AIR G+  SRGE +LM DADGATK  D+EKLE 
Sbjct: 121 VAFKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEK 180

Query: 181 QIHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGP 240
            ++      +             A GSRAHLE++++A R  +R  LM GFH +V      
Sbjct: 181 GLNDLQPWPNQ---------MAIACGSRAHLEKESIAQRSYFRTLLMYGFHFLVWFLCVK 240

Query: 241 GIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSK 300
           GIRDTQCGFK+FTR AA + F+++ ++RW FDVE++Y+ + F IP+ EI+VNW+EI GSK
Sbjct: 241 GIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIEGSK 300

Query: 301 VNPL-SIPNMLWELALMSMGYRTGMWRV 327
           + P  S   M  +L  + + Y TG WR+
Sbjct: 301 LVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of Carg00271 vs. ExPASy Swiss-Prot
Match: Q9DB25 (Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.4e-64
Identity = 125/270 (46.30%), Postives = 176/270 (65.19%), Query Frame = 0

Query: 58  PSIFDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGT 117
           PSI+D   K +S+V+P+YNEE+RLP  + E ++YL++R   D +F+YEV++VDDGS D T
Sbjct: 57  PSIWDSPTKQLSVVVPSYNEEKRLPVMMDEALNYLEKRQKHDCTFTYEVIVVDDGSEDQT 116

Query: 118 KRVGREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKL 177
            +V  ++ +K+  D VRVI L RN GKG A+R G+  SRGE +LM DADGATK  D+EKL
Sbjct: 117 SKVALKYCQKYGSDKVRVITLVRNRGKGGAVRMGVFSSRGEKILMADADGATKFPDVEKL 176

Query: 178 ENQIHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTA 237
           E  +                     A GSRAHLE++++A R  +R FLM GFH +V    
Sbjct: 177 EKGLSDLQPWPEQ---------MAIACGSRAHLEKESIAQRSYFRTFLMYGFHFLVWFLC 236

Query: 238 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPG 297
             GIRDTQCGFK+ TR AA + F+++ ++RW FDVE++Y+ +   IP+ E++VNW+EI G
Sbjct: 237 VKGIRDTQCGFKLLTREAAARTFSSLHIERWAFDVELLYIAQCLQIPIAEVAVNWTEIEG 296

Query: 298 SKVNPL-SIPNMLWELALMSMGYRTGMWRV 327
           SK+ P  S   M  +L  + + Y TG WR+
Sbjct: 297 SKLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of Carg00271 vs. ExPASy Swiss-Prot
Match: A2DSR8 (Dolichyl-phosphate beta-glucosyltransferase ALG5E OS=Trichomonas vaginalis OX=5722 GN=ALG5E PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.3e-61
Identity = 133/339 (39.23%), Postives = 206/339 (60.77%), Query Frame = 0

Query: 3   SLWSLMEVLVYLFLVAACGLLSAIIF---------------EANRRRLNHSHVEAPAVFE 62
           + W+ +E+L+++ +VAA GL+ A++                E N ++LN+ +V+ P    
Sbjct: 2   NFWNFLEILLFIGIVAA-GLIVAVMIKTAEDTTLFDRTQLPEDNPQKLNY-YVQPP---- 61

Query: 63  DPNTSTQVSCPSIFDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVV 122
            PN   +V  P++F+PA+ Y + V+PAYNEE+R+P  L ET++YL+ R AKDKSF++E+V
Sbjct: 62  -PNGDEKVPFPTVFEPAKVYTTFVVPAYNEEKRIPKMLDETVEYLKSREAKDKSFTWEIV 121

Query: 123 IVDDGSIDGTKRVGREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADG 182
           +V+DGS D TK V   ++K +   N+ ++    N GKG AI+ G LH+RGEL+LM+DADG
Sbjct: 122 VVNDGSKDKTKEVVLNYAKDY--PNIFLLNQPVNMGKGAAIQAGCLHARGELVLMVDADG 181

Query: 183 ATKVNDLEKLENQIHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMK 242
           ATK+N+ E LE +I    K  +               GSRA  E+   A R   R FL  
Sbjct: 182 ATKINEFEALETEIKKLMKNNNQ----------AIVVGSRAQNEK---ANRTPIRKFLGL 241

Query: 243 GFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVE 302
           G H++++L+   GI DTQCGFK+F+R A + LF N  ++RWCFD E++ + +R  + + E
Sbjct: 242 GMHVLIVLSGVRGIHDTQCGFKLFSREACKMLFMNQHVQRWCFDPELLVIGRRLGMKISE 301

Query: 303 ISVNWSEIPGSKVNPLSIPNMLWELALMSMGYRTGMWRV 327
           I V W+EI GSK+    +  M  +L  +++ +R G W +
Sbjct: 302 IPVEWNEIEGSKMKISGMIKMAIDLIDIAIFHRVGAWTI 318

BLAST of Carg00271 vs. ExPASy Swiss-Prot
Match: A2DZE8 (Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=5722 GN=ALG5A PE=1 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.1e-60
Identity = 133/331 (40.18%), Postives = 197/331 (59.52%), Query Frame = 0

Query: 5   WSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAP---------AVFEDPNTSTQV 64
           W  +++L +L  VAA GL+ A++            ++ P          +   PN + +V
Sbjct: 4   WRFVQILFFLG-VAAVGLVVAVMIANADDTTLFDRMQLPDGDPNKLNYYIQPAPNGNEKV 63

Query: 65  SCPSIFDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSID 124
             P+IFDPA  Y+SLV+PAYNEE+RLP  L ET++YL+ R  KDKSF++E+VIV+DGS D
Sbjct: 64  PFPTIFDPASVYLSLVVPAYNEEKRLPKMLDETLNYLKSREEKDKSFTWEIVIVNDGSKD 123

Query: 125 GTKRVGREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLE 184
            TK V   ++K++   N+ ++    N GKG AI+ G LH RGEL+LMLDADGATK++D E
Sbjct: 124 KTKEVVLNYAKEY--PNIFLLNQPVNMGKGAAIQAGCLHVRGELVLMLDADGATKIDDFE 183

Query: 185 KLENQIHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLL 244
            LE +I +  K  +               GSRA  E+   A R   R FL  G H +++L
Sbjct: 184 VLEKEIKSLMKTTNQ----------AIVIGSRAQNEK---AKRTPLRKFLSIGMHTLIVL 243

Query: 245 TAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEI 304
           +   GIRDTQCGFK+FTR + + +F N  ++RWC D EI+ + +R  + + E+ V W+EI
Sbjct: 244 SGVHGIRDTQCGFKLFTRESCKMIFMNQHVQRWCCDPEILVIARRLGMKISELPVEWNEI 303

Query: 305 PGSKVNPLSIPNMLWELALMSMGYRTGMWRV 327
            GSK+    +  M  +L  +++ +R G W++
Sbjct: 304 DGSKMKISGMIKMATDLIKIAIFHRVGAWKI 318

BLAST of Carg00271 vs. ExPASy TrEMBL
Match: A0A6J1JT75 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487276 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.6e-183
Identity = 326/328 (99.39%), Postives = 327/328 (99.70%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVL+YLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWSLMEVLIYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERA KDKSFSYEVVIVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAEKDKSFSYEVVIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. ExPASy TrEMBL
Match: A0A6J1GYY1 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111458767 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 6.6e-182
Identity = 324/328 (98.78%), Postives = 327/328 (99.70%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLWSLMEVL+YLFLVAACGLLSAIIFEANRRRLN+S+VEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWSLMEVLIYLFLVAACGLLSAIIFEANRRRLNYSYVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTK V
Sbjct: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKGV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRVHT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 328

BLAST of Carg00271 vs. ExPASy TrEMBL
Match: A0A6J1CEY7 (Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LOC111010109 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.7e-174
Identity = 309/328 (94.21%), Postives = 320/328 (97.56%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLW L+EVL+YL LVAA GLLSA++F+ANRRRLNH+HVEAPAVFEDPNTSTQVSCPSI
Sbjct: 1   MASLWPLLEVLIYLLLVAAFGLLSALLFQANRRRLNHAHVEAPAVFEDPNTSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISL+IPAYNEE RLPGALQETIDYLQERAAKDKSFSYEVVIVDDGS DGTKRV
Sbjct: 61  FDPAEKYISLIIPAYNEELRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSRDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           G +FSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ
Sbjct: 121 GSDFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKES+GSSFR+SDIPVAAFGSRAHLEEKALATRK+YRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESNGSSFRISDIPVAAFGSRAHLEEKALATRKLYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRF IPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFRIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWRV T
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRVDT 328

BLAST of Carg00271 vs. ExPASy TrEMBL
Match: A0A5A7U7Z8 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00140 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 308/328 (93.90%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLW LMEVL+YL LVAA  LLS I+FEANRRRLNHSHVEAPAVF DP TSTQVSCPSI
Sbjct: 1   MASLWPLMEVLIYLLLVAAFILLSLILFEANRRRLNHSHVEAPAVFLDPITSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISL+IPAYNEEQRLPGALQETIDYLQERA KDKSFSYEV+IVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLIIPAYNEEQRLPGALQETIDYLQERATKDKSFSYEVLIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           G EFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATK+ DLEKLENQ
Sbjct: 121 GWEFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKES GS+FRVSDIPVA FGSRAHLEEKALATRK+YRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESIGSNFRVSDIPVATFGSRAHLEEKALATRKVYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWR+HT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRLHT 328

BLAST of Carg00271 vs. ExPASy TrEMBL
Match: A0A1S3CF45 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103500209 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 308/328 (93.90%), Postives = 316/328 (96.34%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           MASLW LMEVL+YL LVAA  LLS I+FEANRRRLNHSHVEAPAVF DP TSTQVSCPSI
Sbjct: 1   MASLWPLMEVLIYLLLVAAFILLSLILFEANRRRLNHSHVEAPAVFLDPITSTQVSCPSI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
           FDPAEKYISL+IPAYNEEQRLPGALQETIDYLQERA KDKSFSYEV+IVDDGSIDGTKRV
Sbjct: 61  FDPAEKYISLIIPAYNEEQRLPGALQETIDYLQERATKDKSFSYEVLIVDDGSIDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
           G EFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATK+ DLEKLENQ
Sbjct: 121 GWEFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAFAKKESSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVVLLTAGPG 240
           IHAFAKKES GS+FRVSDIPVA FGSRAHLEEKALATRK+YRNFLMKGFHLVVLLTAGPG
Sbjct: 181 IHAFAKKESIGSNFRVSDIPVATFGSRAHLEEKALATRKVYRNFLMKGFHLVVLLTAGPG 240

Query: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300
           IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV
Sbjct: 241 IRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWSEIPGSKV 300

Query: 301 NPLSIPNMLWELALMSMGYRTGMWRVHT 329
           NPLSIPNMLWELALMSMGYRTGMWR+HT
Sbjct: 301 NPLSIPNMLWELALMSMGYRTGMWRLHT 328

BLAST of Carg00271 vs. TAIR 10
Match: AT2G39630.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 478.4 bits (1230), Expect = 4.6e-135
Identity = 233/334 (69.76%), Postives = 279/334 (83.53%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           M  L ++ E  ++L L+   G LS ++FEA RRR ++  VE     +DP +   + CP I
Sbjct: 1   MEFLVTVAEFSLWLLLIVLFGFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
            DPAEKY+SL++PAYNEE RLP AL+ET+DYLQ+RA++DKSFS+EVVIVDDGS+DGTKRV
Sbjct: 61  TDPAEKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
             +F +K+++DN+RVI LG+N GKGEAIRKGMLHSRG+LLLMLDADGATKV DLEKLENQ
Sbjct: 121 AFDFIRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQ 180

Query: 181 IHAFAKKE-------SSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYRNFLMKGFHLVV 240
           I+A A++E       S    F++ D+ V+AFGSRAHLEEKALATRK YRNFLMKGFHLVV
Sbjct: 181 INAVAREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALATRKWYRNFLMKGFHLVV 240

Query: 241 LLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVEIVYLCKRFHIPMVEISVNWS 300
           LL AGPGIRDTQCGFKMFTR+AAR+LFTN+ LKRWCFDVE+VYLCKRF+IPMVEISV WS
Sbjct: 241 LLAAGPGIRDTQCGFKMFTRAAARRLFTNVHLKRWCFDVELVYLCKRFNIPMVEISVKWS 300

Query: 301 EIPGSKVNPLSIPNMLWELALMSMGYRTGMWRVH 328
           EIPGSKV+ LSIPNMLWELALMS+GYRTGMW++H
Sbjct: 301 EIPGSKVSMLSIPNMLWELALMSVGYRTGMWKIH 334

BLAST of Carg00271 vs. TAIR 10
Match: AT2G39630.2 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-72
Identity = 138/229 (60.26%), Postives = 176/229 (76.86%), Query Frame = 0

Query: 1   MASLWSLMEVLVYLFLVAACGLLSAIIFEANRRRLNHSHVEAPAVFEDPNTSTQVSCPSI 60
           M  L ++ E  ++L L+   G LS ++FEA RRR ++  VE     +DP +   + CP I
Sbjct: 1   MEFLVTVAEFSLWLLLIVLFGFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHI 60

Query: 61  FDPAEKYISLVIPAYNEEQRLPGALQETIDYLQERAAKDKSFSYEVVIVDDGSIDGTKRV 120
            DPAEKY+SL++PAYNEE RLP AL+ET+DYLQ+RA++DKSFS+EVVIVDDGS+DGTKRV
Sbjct: 61  TDPAEKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRV 120

Query: 121 GREFSKKFSVDNVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKVNDLEKLENQ 180
             +F +K+++DN+RVI LG+N GKGEAIRKGMLHSRG+LLLMLDADGATKV DLEKLENQ
Sbjct: 121 AFDFIRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQ 180

Query: 181 IHAFAKKE-------SSGSSFRVSDIPVAAFGSRAHLEEKALATRKMYR 223
           I+A A++E       S    F++ D+ V+AFGSRAHLEEKALAT   +R
Sbjct: 181 INAVAREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALATSFNFR 229

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7031614.16.6e-184100.00Dolichyl-phosphate beta-glucosyltransferase [Cucurbita argyrosperma subsp. argyr... [more]
KAG6601000.18.6e-18499.70Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma sub... [more]
XP_022990413.13.3e-18399.39dolichyl-phosphate beta-glucosyltransferase-like [Cucurbita maxima][more]
XP_023518326.14.3e-18399.39dolichyl-phosphate beta-glucosyltransferase [Cucurbita pepo subsp. pepo][more]
XP_022957336.11.4e-18198.78dolichyl-phosphate beta-glucosyltransferase-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9VLQ11.7e-6543.43Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 G... [more]
Q9Y6732.2e-6543.29Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1... [more]
Q9DB252.4e-6446.30Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=... [more]
A2DSR83.3e-6139.23Dolichyl-phosphate beta-glucosyltransferase ALG5E OS=Trichomonas vaginalis OX=57... [more]
A2DZE82.1e-6040.18Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=57... [more]
Match NameE-valueIdentityDescription
A0A6J1JT751.6e-18399.39Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1GYY16.6e-18298.78Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1CEY71.7e-17494.21Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LO... [more]
A0A5A7U7Z81.3e-17293.90Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CF451.3e-17293.90Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103500... [more]
Match NameE-valueIdentityDescription
AT2G39630.14.6e-13569.76Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT2G39630.21.5e-7260.26Nucleotide-diphospho-sugar transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001173Glycosyltransferase 2-likePFAMPF00535Glycos_transf_2coord: 69..259
e-value: 2.6E-24
score: 85.9
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 50..315
e-value: 1.0E-35
score: 125.6
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 65..301
NoneNo IPR availablePANTHERPTHR10859GLYCOSYL TRANSFERASEcoord: 3..327
NoneNo IPR availablePANTHERPTHR10859:SF108BNAA03G18660D PROTEINcoord: 3..327
IPR035518Dolichyl-phosphate beta-glucosyltransferaseCDDcd04188DPG_synthasecoord: 70..299
e-value: 1.13864E-107
score: 311.039

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00271-RACarg00271-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004581 dolichyl-phosphate beta-glucosyltransferase activity