Carg00268 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00268
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	CASP-like protein
Location	Carg_Chr04: 6292892 .. 6293997 (-)
RNA-Seq Expression	Carg00268
Synteny	Carg00268

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AACAATCAATTTCACCCCCATTTCATGTATGACCAACTACTCTACCTGTAGCAAACTGCGACGGCGACCATGCGATCCCCTCAGTCACTCCGTAACGGCGGCGGCACACCATCGCCTCACGCTCGAATCCCTACTCCTCACCATCACTTTCACTCGACCGTCTCTGTTCAGAAACTGAAGCGCTTCAACTCTCTAATCCTCGTCTTCCGTTTGTCTACCTTCTGCTTCTCCCTTGCCTCTGCTGTTTTCATGATTACTAACTCTCGCGGCTCTGGATCCGATTCCCCTCGCTGGTACGATTTCGACGCCTTCAGGTACGCCGTTTGCCCTATTTTCCGATTCTTCGCATTCGAGGTATTTCTCCTGAAAATCAAATACAACAGACGAGATTTTCGAATTGGAACCACAACTAATTCCGCACATACGGAAACGCACATTTCCTTACTAAAAAACGCACTCTATTACCGAACTAACGCTTTCCTGACCAGGTACGTCTTCGCGGCAAACGCCATAGTCGCAGTGTACTCACTGTTCGAAATGATTGCCTCCGTGTGGGAAATCTCGAGAGAAGTGACGCTCTTCCCTGAGATTTTACAAGTCTGGTTCGACTTCGGCCACGACCAGGTCAAAACCCTAACCCTTTGCCTTTTTTCTCTTTTCTCTATCTTCTGGTCTAAATCGGAAATTAGGGCTCTGATTCCATAGGCGTTCGCGTACCTTCTTCTCTCTGCGGGCTCCGCAGGGACGGCTCTCTTGATAACTCTGAGGGGAACCGACACGTGTAGGGCCACCAGCGCCTTTTGTGTTCAATCCACGATCTCGATTGCATTGGGATTCGCCGGTTTCCTGTTTCTCGGGTTATCCTCGCTGCTTTCGGGTTTTCGGGTCGTCTGTTTCGTAATCAACGGCTCTCGTTTTCATTTATAACCTTACTTCCTTGAAGAGGAGTTCCACCGCTGAATTTTTTGGTGCGTCATGTCGTTTGATTTGCCTAATTATATCTAATGGTCCTTCATCTTTTTCTTTTTCTCCATTTGGTCCTTAATTTATCTAAAATTAACAACTCTCAATTATATCTAATTAGTTTAAACATTAAATTAAATTTT

mRNA sequence

AACAATCAATTTCACCCCCATTTCATGTATGACCAACTACTCTACCTGTAGCAAACTGCGACGGCGACCATGCGATCCCCTCAGTCACTCCGTAACGGCGGCGGCACACCATCGCCTCACGCTCGAATCCCTACTCCTCACCATCACTTTCACTCGACCGTCTCTGTTCAGAAACTGAAGCGCTTCAACTCTCTAATCCTCGTCTTCCGTTTGTCTACCTTCTGCTTCTCCCTTGCCTCTGCTGTTTTCATGATTACTAACTCTCGCGGCTCTGGATCCGATTCCCCTCGCTGGTACGATTTCGACGCCTTCAGGTACGTCTTCGCGGCAAACGCCATAGTCGCAGTGTACTCACTGTTCGAAATGATTGCCTCCGTGTGGGAAATCTCGAGAGAAGTGACGCTCTTCCCTGAGATTTTACAAGTCTGGTTCGACTTCGGCCACGACCAGGCGTTCGCGTACCTTCTTCTCTCTGCGGGCTCCGCAGGGACGGCTCTCTTGATAACTCTGAGGGGAACCGACACGTGTAGGGCCACCAGCGCCTTTTGTGTTCAATCCACGATCTCGATTGCATTGGGATTCGCCGGTTTCCTGTTTCTCGGGTTATCCTCGCTGCTTTCGGGTTTTCGGGTCGTCTGTTTCGTAATCAACGGCTCTCGTTTTCATTTATAACCTTACTTCCTTGAAGAGGAGTTCCACCGCTGAATTTTTTGGTGCGTCATGTCGTTTGATTTGCCTAATTATATCTAATGGTCCTTCATCTTTTTCTTTTTCTCCATTTGGTCCTTAATTTATCTAAAATTAACAACTCTCAATTATATCTAATTAGTTTAAACATTAAATTAAATTTT

Coding sequence (CDS)

ATGCGATCCCCTCAGTCACTCCGTAACGGCGGCGGCACACCATCGCCTCACGCTCGAATCCCTACTCCTCACCATCACTTTCACTCGACCGTCTCTGTTCAGAAACTGAAGCGCTTCAACTCTCTAATCCTCGTCTTCCGTTTGTCTACCTTCTGCTTCTCCCTTGCCTCTGCTGTTTTCATGATTACTAACTCTCGCGGCTCTGGATCCGATTCCCCTCGCTGGTACGATTTCGACGCCTTCAGGTACGTCTTCGCGGCAAACGCCATAGTCGCAGTGTACTCACTGTTCGAAATGATTGCCTCCGTGTGGGAAATCTCGAGAGAAGTGACGCTCTTCCCTGAGATTTTACAAGTCTGGTTCGACTTCGGCCACGACCAGGCGTTCGCGTACCTTCTTCTCTCTGCGGGCTCCGCAGGGACGGCTCTCTTGATAACTCTGAGGGGAACCGACACGTGTAGGGCCACCAGCGCCTTTTGTGTTCAATCCACGATCTCGATTGCATTGGGATTCGCCGGTTTCCTGTTTCTCGGGTTATCCTCGCTGCTTTCGGGTTTTCGGGTCGTCTGTTTCGTAATCAACGGCTCTCGTTTTCATTTATAA

Protein sequence

MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVWFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLSSLLSGFRVVCFVINGSRFHL

Homology

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BLAST of Carg00268 vs. NCBI nr
Match: XP_023524861.1 (CASP-like protein 4C1 [Cucurbita pepo subsp. pepo] >KAG6600997.1 CASP-like protein 4C2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031611.1 CASP-like protein 4C2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.5 bits (1007), Expect = 2.2e-105
Identity = 200/200 (100.00%), Postives = 200/200 (100.00%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF
Sbjct: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW
Sbjct: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS
Sbjct: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SLLSGFRVVCFVINGSRFHL
Sbjct: 181 SLLSGFRVVCFVINGSRFHL 200

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BLAST of Carg00268 vs. NCBI nr
Match: XP_022957593.1 (CASP-like protein 4C1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 389.4 bits (999), Expect = 1.8e-104
Identity = 199/200 (99.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF
Sbjct: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW
Sbjct: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQAFAYLLLSAGSAGTALLITLR TDTCRATSAFCVQSTISIALGFAGFLFLGLS
Sbjct: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRRTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SLLSGFRVVCFVINGSRFHL
Sbjct: 181 SLLSGFRVVCFVINGSRFHL 200

[Close]

BLAST of Carg00268 vs. NCBI nr
Match: XP_022991395.1 (CASP-like protein 4C1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 384.8 bits (987), Expect = 4.5e-103
Identity = 198/200 (99.00%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSPQSLRN GGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF
Sbjct: 1   MRSPQSLRN-GGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVT+FPEILQVW
Sbjct: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTIFPEILQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS
Sbjct: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SLLSGFRVVCFVINGSRFHL
Sbjct: 181 SLLSGFRVVCFVINGSRFHL 199

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BLAST of Carg00268 vs. NCBI nr
Match: XP_004147690.1 (CASP-like protein 4C1 [Cucumis sativus] >KGN50549.1 hypothetical protein Csa_021394 [Cucumis sativus])

HSP 1 Score: 376.7 bits (966), Expect = 1.2e-100
Identity = 194/203 (95.57%), Postives = 196/203 (96.55%), Query Frame = 0


Query: 1   MRSPQSLRN----GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLA 60
           MRSPQSLRN    GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLA
Sbjct: 1   MRSPQSLRNGGVGGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLA 60

Query: 61  SAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEI 120
           SAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEI
Sbjct: 61  SAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEI 120

Query: 121 LQVWFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLF 180
           LQVWFDFGHDQAFAYLLLSA SAGTAL ITL+GTDTC+ TSAFCVQSTISIALGFAGFLF
Sbjct: 121 LQVWFDFGHDQAFAYLLLSADSAGTALAITLKGTDTCKVTSAFCVQSTISIALGFAGFLF 180

Query: 181 LGLSSLLSGFRVVCFVINGSRFH 200
           LGLSSLLSGFRVVCFVINGSRFH
Sbjct: 181 LGLSSLLSGFRVVCFVINGSRFH 203

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BLAST of Carg00268 vs. NCBI nr
Match: XP_038893216.1 (CASP-like protein 4C1 [Benincasa hispida])

HSP 1 Score: 376.3 bits (965), Expect = 1.6e-100
Identity = 194/204 (95.10%), Postives = 196/204 (96.08%), Query Frame = 0


Query: 1   MRSPQSLRN-----GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSL 60
           MRSPQSLRN     GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSL
Sbjct: 1   MRSPQSLRNGGAGGGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSL 60

Query: 61  ASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPE 120
           ASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPE
Sbjct: 61  ASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPE 120

Query: 121 ILQVWFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFL 180
           ILQVWFDFGHDQAFAYLLLSA SAGTAL ITL+GTDTC+ TSAFCVQSTISIALGFAGFL
Sbjct: 121 ILQVWFDFGHDQAFAYLLLSADSAGTALAITLKGTDTCKVTSAFCVQSTISIALGFAGFL 180

Query: 181 FLGLSSLLSGFRVVCFVINGSRFH 200
           FLGLSSLLSGFRVVCFVINGSRFH
Sbjct: 181 FLGLSSLLSGFRVVCFVINGSRFH 204

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BLAST of Carg00268 vs. ExPASy Swiss-Prot
Match: B9SR15 (CASP-like protein 4C1 OS=Ricinus communis OX=3988 GN=RCOM_0464280 PE=2 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.2e-77
Identity = 159/201 (79.10%), Postives = 170/201 (84.58%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPT-PHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAV 60
           MRSPQSLRN G TPSP  R P  P  HFHSTVS+QKLKRFN LILVFRLSTFCFSLAS+V
Sbjct: 1   MRSPQSLRN-GETPSPSPRPPRFPTPHFHSTVSLQKLKRFNLLILVFRLSTFCFSLASSV 60

Query: 61  FMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQV 120
           FM+TN        P WY FDAFRYVFAANAIVA+YSLFEM ASVWEISR  TLFPEILQV
Sbjct: 61  FMLTN--------PTWYHFDAFRYVFAANAIVAIYSLFEMAASVWEISRGNTLFPEILQV 120

Query: 121 WFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGL 180
           WFDFGHDQ FAYLLLSA SA TAL  TL+G DTC A++AFCVQS I+IALGFAGFLFLGL
Sbjct: 121 WFDFGHDQVFAYLLLSADSAATALAKTLKGGDTCAASNAFCVQSYIAIALGFAGFLFLGL 180

Query: 181 SSLLSGFRVVCFVINGSRFHL 201
           SSLLSGFRVVCF+INGSRF++
Sbjct: 181 SSLLSGFRVVCFLINGSRFYV 192

[Close]

BLAST of Carg00268 vs. ExPASy Swiss-Prot
Match: B9HTK2 (CASP-like protein 4C2 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_822486 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 1.5e-72
Identity = 148/200 (74.00%), Postives = 160/200 (80.00%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSPQ  R+GG T            HF STVSVQKLKRFNSLILVFR + FCFSLASAVF
Sbjct: 1   MRSPQPHRSGGDT----------QQHFQSTVSVQKLKRFNSLILVFRFAAFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           M+TNSR  GSDS  WY+FDAFRYVFAANAIVA+YSLFEM ASVWEISR  TLFPEI QVW
Sbjct: 61  MLTNSR--GSDSLHWYNFDAFRYVFAANAIVAIYSLFEMAASVWEISRNATLFPEICQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQ FAYLLLSA +AGT L  TL+  DTC    AFCVQS I+I LGFAGFLFLG+S
Sbjct: 121 FDFGHDQVFAYLLLSANTAGTELARTLK--DTCTDNKAFCVQSDIAIVLGFAGFLFLGIS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SL SGFRVVCF+INGSRF++
Sbjct: 181 SLFSGFRVVCFIINGSRFYV 186

[Close]

BLAST of Carg00268 vs. ExPASy Swiss-Prot
Match: B9HMF8 (CASP-like protein 4C1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_820327 PE=3 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 8.1e-71
Identity = 143/200 (71.50%), Postives = 157/200 (78.50%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MR PQ  RNGG T            HFHST+S+QKLKRFNSLILVFR S FCFSLASAVF
Sbjct: 1   MRFPQPHRNGGET----------QQHFHSTISLQKLKRFNSLILVFRFSAFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           M+TNSR  GSDS  WY+FDAFRYVFAANAIVAVYSLFEM A+VWEISR  TLFPE+ QVW
Sbjct: 61  MLTNSR--GSDSFHWYNFDAFRYVFAANAIVAVYSLFEMTAAVWEISRNATLFPEVCQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQ FAYLLLSA S GT +  T++  D C     FCVQS I+IALGF GFLFLG+S
Sbjct: 121 FDFGHDQVFAYLLLSANSTGTEMARTMK--DACTDNKPFCVQSDIAIALGFVGFLFLGVS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SL SGFRVVCF+INGSRF++
Sbjct: 181 SLFSGFRVVCFIINGSRFYV 186

[Close]

BLAST of Carg00268 vs. ExPASy Swiss-Prot
Match: Q9M2U0 (CASP-like protein 4C1 OS=Arabidopsis thaliana OX=3702 GN=At3g55390 PE=2 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 8.4e-68
Identity = 137/200 (68.50%), Postives = 154/200 (77.00%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSP + RNG    SP  R    H HFHSTV+ QKL+RFNSLIL+ RL++F FSLASAVF
Sbjct: 1   MRSPHAFRNG---ESPTLR---DHTHFHSTVTAQKLRRFNSLILLLRLASFSFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           M+TNSRGS   SP WYDFDAFR+VF ANAIVA+YS+FEM   VWE SRE TL+PE  QVW
Sbjct: 61  MLTNSRGSA--SPHWYDFDAFRFVFVANAIVALYSVFEMGTCVWEFSRETTLWPEAFQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQ F+YLLLSAGSA  AL  T+RG DTC A  AFC+QS ++I LGFA FLFL  S
Sbjct: 121 FDFGHDQVFSYLLLSAGSAAAALARTMRGGDTCTANKAFCLQSDVAIGLGFAAFLFLAFS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           S  SGFRV CF+I GSRFHL
Sbjct: 181 SCFSGFRVACFLITGSRFHL 192

[Close]

BLAST of Carg00268 vs. ExPASy Swiss-Prot
Match: A9T836 (CASP-like protein 4C3 OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_192523 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.1e-34
Identity = 81/171 (47.37%), Postives = 100/171 (58.48%), Query Frame = 0


Query: 26  HFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVFMITN-SRGSGSDSPRWYDFDAFRYV 85
           H++   + QK +R N +IL+FR  TF FSL S + M TN  R        WYDFD FRYV
Sbjct: 15  HYYGKSTAQKHRRSNGIILIFRALTFSFSLTSVIVMGTNRHRIDAQSRVAWYDFDPFRYV 74

Query: 86  FAANAIVAVYSLFEMIASVWEISREVTLFPEILQVWFDFGHDQAFAYLLLSAGSAGTALL 145
            A NAI+ +YS  E+  +V+   ++    PE  QVWFD+GHDQ FAYLL SA SAG A+ 
Sbjct: 75  LAVNAIICIYSFVEIWLAVYTYLKDTLFLPETFQVWFDYGHDQGFAYLLFSANSAGIAMA 134

Query: 146 ITLR-------GTDTCRATSAFCVQSTISIALGFAGFLFLGLSSLLSGFRV 189
             L+       G   C     FC Q+  SI LGF  FLFL LSSLL+G RV
Sbjct: 135 QLLQSGNSLIHGAYRCSDAGVFCTQARASIGLGFGAFLFLALSSLLTGLRV 185

[Close]

BLAST of Carg00268 vs. ExPASy TrEMBL
Match: A0A6J1H0N7 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458937 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 8.9e-105
Identity = 199/200 (99.50%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF
Sbjct: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW
Sbjct: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQAFAYLLLSAGSAGTALLITLR TDTCRATSAFCVQSTISIALGFAGFLFLGLS
Sbjct: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRRTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SLLSGFRVVCFVINGSRFHL
Sbjct: 181 SLLSGFRVVCFVINGSRFHL 200

[Close]

BLAST of Carg00268 vs. ExPASy TrEMBL
Match: A0A6J1JST8 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488036 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.2e-103
Identity = 198/200 (99.00%), Postives = 199/200 (99.50%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSPQSLRN GGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF
Sbjct: 1   MRSPQSLRN-GGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVT+FPEILQVW
Sbjct: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTIFPEILQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS
Sbjct: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           SLLSGFRVVCFVINGSRFHL
Sbjct: 181 SLLSGFRVVCFVINGSRFHL 199

[Close]

BLAST of Carg00268 vs. ExPASy TrEMBL
Match: A0A0A0KNW2 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G182050 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 6.0e-101
Identity = 194/203 (95.57%), Postives = 196/203 (96.55%), Query Frame = 0


Query: 1   MRSPQSLRN----GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLA 60
           MRSPQSLRN    GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLA
Sbjct: 1   MRSPQSLRNGGVGGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLA 60

Query: 61  SAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEI 120
           SAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEI
Sbjct: 61  SAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEI 120

Query: 121 LQVWFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLF 180
           LQVWFDFGHDQAFAYLLLSA SAGTAL ITL+GTDTC+ TSAFCVQSTISIALGFAGFLF
Sbjct: 121 LQVWFDFGHDQAFAYLLLSADSAGTALAITLKGTDTCKVTSAFCVQSTISIALGFAGFLF 180

Query: 181 LGLSSLLSGFRVVCFVINGSRFH 200
           LGLSSLLSGFRVVCFVINGSRFH
Sbjct: 181 LGLSSLLSGFRVVCFVINGSRFH 203

[Close]

BLAST of Carg00268 vs. ExPASy TrEMBL
Match: A0A5D3BVM1 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00170 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.1e-100
Identity = 193/206 (93.69%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MRSPQSLRN-------GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCF 60
           MRSP SLRN       GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCF
Sbjct: 1   MRSPHSLRNGGGGGGGGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCF 60

Query: 61  SLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLF 120
           SLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLF
Sbjct: 61  SLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLF 120

Query: 121 PEILQVWFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAG 180
           PEILQVWFDFGHDQAFAYLLLSA SAGTAL ITL+GTDTC+ TSAFCVQSTISIALGFAG
Sbjct: 121 PEILQVWFDFGHDQAFAYLLLSADSAGTALAITLKGTDTCKVTSAFCVQSTISIALGFAG 180

Query: 181 FLFLGLSSLLSGFRVVCFVINGSRFH 200
           FLFLGLSSLLSGFRVVCFVINGSRFH
Sbjct: 181 FLFLGLSSLLSGFRVVCFVINGSRFH 206

[Close]

BLAST of Carg00268 vs. ExPASy TrEMBL
Match: A0A1S3CF55 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103500218 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.1e-100
Identity = 193/206 (93.69%), Postives = 195/206 (94.66%), Query Frame = 0


Query: 1   MRSPQSLRN-------GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCF 60
           MRSP SLRN       GGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCF
Sbjct: 1   MRSPHSLRNGGGGGGGGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCF 60

Query: 61  SLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLF 120
           SLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLF
Sbjct: 61  SLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLF 120

Query: 121 PEILQVWFDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAG 180
           PEILQVWFDFGHDQAFAYLLLSA SAGTAL ITL+GTDTC+ TSAFCVQSTISIALGFAG
Sbjct: 121 PEILQVWFDFGHDQAFAYLLLSADSAGTALAITLKGTDTCKVTSAFCVQSTISIALGFAG 180

Query: 181 FLFLGLSSLLSGFRVVCFVINGSRFH 200
           FLFLGLSSLLSGFRVVCFVINGSRFH
Sbjct: 181 FLFLGLSSLLSGFRVVCFVINGSRFH 206

[Close]

BLAST of Carg00268 vs. TAIR 10
Match: AT3G55390.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 258.1 bits (658), Expect = 6.0e-69
Identity = 137/200 (68.50%), Postives = 154/200 (77.00%), Query Frame = 0


Query: 1   MRSPQSLRNGGGTPSPHARIPTPHHHFHSTVSVQKLKRFNSLILVFRLSTFCFSLASAVF 60
           MRSP + RNG    SP  R    H HFHSTV+ QKL+RFNSLIL+ RL++F FSLASAVF
Sbjct: 1   MRSPHAFRNG---ESPTLR---DHTHFHSTVTAQKLRRFNSLILLLRLASFSFSLASAVF 60

Query: 61  MITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVW 120
           M+TNSRGS   SP WYDFDAFR+VF ANAIVA+YS+FEM   VWE SRE TL+PE  QVW
Sbjct: 61  MLTNSRGSA--SPHWYDFDAFRFVFVANAIVALYSVFEMGTCVWEFSRETTLWPEAFQVW 120

Query: 121 FDFGHDQAFAYLLLSAGSAGTALLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLS 180
           FDFGHDQ F+YLLLSAGSA  AL  T+RG DTC A  AFC+QS ++I LGFA FLFL  S
Sbjct: 121 FDFGHDQVFSYLLLSAGSAAAALARTMRGGDTCTANKAFCLQSDVAIGLGFAAFLFLAFS 180

Query: 181 SLLSGFRVVCFVINGSRFHL 201
           S  SGFRV CF+I GSRFHL
Sbjct: 181 SCFSGFRVACFLITGSRFHL 192

[Close]

BLAST of Carg00268 vs. TAIR 10
Match: AT1G79780.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 62.0 bits (149), Expect = 6.3e-10
Identity = 40/120 (33.33%), Postives = 60/120 (50.00%), Query Frame = 0


Query: 72  SPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVWFDFGHDQAFAY 131
           S  W    +++YV  A A   +YSL ++   V+ +     + P   Q W  F  DQ F Y
Sbjct: 61  SSNWSFSPSYQYVVGACAGTVLYSLLQLCLGVYRLVTGSPITPSRFQAWLCFTSDQLFCY 120

Query: 132 LLLSAGSAGTALL----ITLRGT---DTCRATSAFCVQSTISIALGFAGFLFLGLSSLLS 185
           L++SAGSAG+ +       +R T   D C+  S+FC    +S+ L F  F+FL  SS  +
Sbjct: 121 LMMSAGSAGSGVTNLNKTGIRHTPLPDFCKTLSSFCNHVALSLLLVFLSFIFLASSSFFT 180

[Close]

BLAST of Carg00268 vs. TAIR 10
Match: AT1G17200.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 52.8 bits (125), Expect = 3.8e-07
Identity = 43/151 (28.48%), Postives = 75/151 (49.67%), Query Frame = 0


Query: 45  VFRLSTFCFSLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVW 104
           + RL+     +A+ V M+ +S  +   S  + +  AFRY+  AN I A YSL  + A++ 
Sbjct: 36  MLRLAPVGLCVAALVVMLKDSETNEFGSISYSNLTAFRYLVHANGICAGYSL--LSAAIA 95

Query: 105 EISREVTLFPEILQVWFDFGHDQAFAYLLLSAGSAGTALLI-------TLRGTDTCRATS 164
            + R  +  P   +VW  F  DQ   YL+L+AG+    +L         +  +D C +  
Sbjct: 96  AMPRSSSTMP---RVWTFFCLDQLLTYLVLAAGAVSAEVLYLAYNGDSAITWSDACSSYG 155

Query: 165 AFCVQSTISIALGFAGFLFLGLSSLLSGFRV 189
            FC ++T S+ + F    F  + SL+S +++
Sbjct: 156 GFCHRATASVIITFFVVCFYIVLSLISSYKL 181

[Close]

BLAST of Carg00268 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 52.4 bits (124), Expect = 5.0e-07
Identity = 48/137 (35.04%), Postives = 73/137 (53.28%), Query Frame = 0


Query: 53  FSLASAVFMITNSRGSGSDSPRWYDFDAFRYVFAANAIVAVYSLFEMIASVWEISREVTL 112
           FSL + + M++N  G G +   + D++ +RYV A + I  +Y+ ++  A      RE+  
Sbjct: 58  FSLIAFLIMVSNKHGYGRN---FNDYEEYRYVLAISIISTLYTAWQTFAHF--SKREI-- 117

Query: 113 FPEILQVWFDFGHDQAFAYLLLSAGSAGTALLITLR-GTDTCRATSAFCVQSTISIALGF 172
           F     +  DF  DQ  AYLL+SA S+   L    R G D     SA    S IS+A+ F
Sbjct: 118 FDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFREGQDNIFTDSA---ASAISMAI-F 177

Query: 173 AGFLFLGLSSLLSGFRV 189
           A F+ L LS+L SG+++
Sbjct: 178 A-FIALALSALFSGYKL 182

[Close]

BLAST of Carg00268 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 52.0 bits (123), Expect = 6.5e-07
Identity = 50/164 (30.49%), Postives = 77/164 (46.95%), Query Frame = 0


Query: 27  FHSTVSVQKLKRFNSLI----LVFRLSTFCFSLASAVFMITNSRGSGSDSPRWYDFDAFR 86
           F S+ S  +  ++ SLI    L FR+  F   L S   M+++ R  G     +Y++  FR
Sbjct: 103 FSSSSSTPRESKWASLIRKALLGFRVIAFVSCLVSFSVMVSD-RDKGWAHDSFYNYKEFR 162

Query: 87  YVFAANAIVAVYSLFEMIASVWEISREVTLFPEILQVWFDFGHDQAFAYLLLSAGSAGTA 146
           +  AAN I  VYS F +   V+ +S  +      L+ + +FG DQ  AYLL    SA T+
Sbjct: 163 FCLAANVIGFVYSGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLL---ASASTS 222

Query: 147 LLITLRGTDTCRATSAFCVQSTISIALGFAGFLFLGLSSLLSGF 187
             I +    +      F   +  S+AL +  F+     SL SG+
Sbjct: 223 ASIRVDDWQSNWGADKFPDLARASVALSYVSFVAFAFCSLASGY 262

The following BLAST results are available for this feature:

BLAST of Carg00268 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524861.1	2.2e-105	100.00	CASP-like protein 4C1 [Cucurbita pepo subsp. pepo] >KAG6600997.1 CASP-like prote...	[more]
XP_022957593.1	1.8e-104	99.50	CASP-like protein 4C1 isoform X2 [Cucurbita moschata]	[more]
XP_022991395.1	4.5e-103	99.00	CASP-like protein 4C1 isoform X2 [Cucurbita maxima]	[more]
XP_004147690.1	1.2e-100	95.57	CASP-like protein 4C1 [Cucumis sativus] >KGN50549.1 hypothetical protein Csa_021...	[more]
XP_038893216.1	1.6e-100	95.10	CASP-like protein 4C1 [Benincasa hispida]	[more]

BLAST of Carg00268 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9SR15	1.2e-77	79.10	CASP-like protein 4C1 OS=Ricinus communis OX=3988 GN=RCOM_0464280 PE=2 SV=1	[more]
B9HTK2	1.5e-72	74.00	CASP-like protein 4C2 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_822486 PE=3 S...	[more]
B9HMF8	8.1e-71	71.50	CASP-like protein 4C1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_820327 PE=3 S...	[more]
Q9M2U0	8.4e-68	68.50	CASP-like protein 4C1 OS=Arabidopsis thaliana OX=3702 GN=At3g55390 PE=2 SV=1	[more]
A9T836	6.1e-34	47.37	CASP-like protein 4C3 OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_192523 PE=2 ...	[more]

BLAST of Carg00268 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H0N7	8.9e-105	99.50	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111458937 PE=3 SV=1	[more]
A0A6J1JST8	2.2e-103	99.00	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111488036 PE=3 SV=1	[more]
A0A0A0KNW2	6.0e-101	95.57	CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G182050 PE=3 SV=1	[more]
A0A5D3BVM1	5.1e-100	93.69	CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00...	[more]
A0A1S3CF55	5.1e-100	93.69	CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103500218 PE=3 SV=1	[more]

BLAST of Carg00268 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55390.1	6.0e-69	68.50	Uncharacterised protein family (UPF0497)	[more]
AT1G79780.1	6.3e-10	33.33	Uncharacterised protein family (UPF0497)	[more]
AT1G17200.1	3.8e-07	28.48	Uncharacterised protein family (UPF0497)	[more]
AT2G38480.1	5.0e-07	35.04	Uncharacterised protein family (UPF0497)	[more]
AT5G40300.1	6.5e-07	30.49	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006459	Casparian strip membrane protein	TIGRFAM	TIGR01569	TIGR01569	coord: 44..184 e-value: 7.4E-12 score: 43.3
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 40..176 e-value: 1.3E-30 score: 106.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR11615:SF236	CASP-LIKE PROTEIN 4C1	coord: 1..200
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 1..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00268-RA	Carg00268-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding