Carg00171 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00171
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Alpha-galactosidase
Location	Carg_Chr04: 5723103 .. 5727802 (+)
RNA-Seq Expression	Carg00171
Synteny	Carg00171

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGAGGCAACCGAACACACGGAAAAGCTAGCATTTCATGGCTTTGATAAATCGATTCTTGCATCAAAGTACCTTCAAATTTATAACAACTCCAAATATGGAATTTTGCAGCTCGACAATGGTTTGGGAAAAACTCCTCCCATGGGGTCTCTCTCTTTCCTTTTCTCTCAATCTCTCATCCCTTTTCTTTCTAATAACAGTGTTAATAACAGTGTTTTTATGCCATCTTTTCTTTTGCTTATCAGATGGAATAGTTGGAACCATTTTGGCTGCAGTATAGATGAAAGGGTCATCAAAGAAACTGGTATTCTCTCTTTTTAGGTTCGGGTTCGTTCCACCTGTTTAACAATACGTACGATGAAAGATTTGAATCTCTTATCTCTTTTTAAAATTTTAAGAAGGGAGGTTATTAGAATAAGAAAGGTTAACCAAGACTATTCTAAAAATTTATTGAAATTACAACTATTTATTCCTTTTTATAGCGGATGCACTTGTTGCTACGGGACTGGCTGAGTTAGGCTATGTATACGTCAACATAGGTAATTTTTCCATCATTTGCTTTTACCACAAACATCTTCTATCTCGATTAAATTAAAATGGTAATATTTCAATTTATATTCTGTAACTTTCAATTTTGTATTTGACCATGGAGCATACGTGTATAGTAAATCTTTAAACTTTCAATTTTGTATATAATAGATTTGAATTTTTTCTAAAAGCTTCTCGTTGCTATTTGAATTTTAAAAAATAGTTGAATGACACTCAATTTATTAAATATAAAACTAAAATTTTATGTAAATTTATTAAATATTTTTTTAAAAAAATCAGAATATAAAATTTTAAAATTATACTTGTAATTTAGTTTAGGTTATATTTTAAAATTTACCATATGTTTCAAACAATGGCAGATGATTGCTGGGCCAACCAACAGAGAATTCTGGTAATCTCTCCTTACTATTCTATCTATTCCAAGAATATATATATATTTTGTTGCGAAAAATTATAAGTTTAGTTCCAACATTTATAAAAATATATAATATAAACATAAAAATTATATATATATATATATATATATAGATTTGCTAAATTATATAAAATATACTTTAAAATATTTTTACAATTTTACTCTTAAACTAATTTATTTCTTTAAAAAAATATTAGTTTTTAAATAAAAATTATTAATAATTTATTTAAAAAATAACTTAAAATTTTTAGTATCACTATCATGTTTAAAAAATATCTCCCTCTTCACTCCCTCAATTTATGAACAAGTACTTTTCCAATTTTCCTTCATTTATTTGATGCTCTCCCGATCCTTTTCGTTCAATCTCCAATCTTAAAACTGCTCCAATTTTTCTATTTTTTTCTCTTCGTAAATTATCATAGTTTTAAACAAGTATAATAAGTTGTCCTATTTTTTAATGTCTAAACATAAATATAAAAAGTTTTAATATCAAAAGTGTCAAATTTTAAAATTTATATTGGGTTGAAAATTAACTCATATACATACCTTCATTTTTTTTTTTTTCTTGTTCAATTCTTACGTCAATCTCTGAGCAAACAAACATAACTCAAAACTTATAACATCTCAAAATCAAAATCTTCTCTCAAAACTCAACAAATACAAGAAAGGTCCACAAATGAATAGAATGTTTGATTATGCTAACCTTTTTAGCTCAACATAACAAATCAACTAATTAAACTATAAAATGAATGAATGAGTGAAGTGTCCACTGGTTGTGCAGGTTTTTCTGGCTCCTGATCTCAAAACTTTTCCCTCAGGGATTAAAGCCCTTGCTGATTATGTACATGGGAAAGGCTTGAAGCTTGGAATATATGGTGATGCTGGGTACGACCGATGTCTAGCTCATCTTTTAATGTTAAAAAAACTTTAATATTGTCTTTCTTTCTCTCTCGATCGCTTGTTTCATTAACTTGCCATACTGTTGAACAAGGCAATTAACTTGCCAGAAGCGACCAGGGTCGCTCGGCTTTGAACTTTGGGATGCAATGATGCTAGCATCTTGGGTGAGTAATTTCCATCTTATAACCTAGCTTCGATATTCAACTGTTCTGTTTATCGAATGGGTACTAACAAATTTGTAAAAATATAAAGGTAACGAAGTTTCTAGTTTTTTGAAAATTAAGTTTATAAATACGGATTCCCGTCTCGAGATTTTGTTTTTTTCTTCAGGAGGTGGATTATTTGAAGTATGATAACTGTTATGATATGGGAATCAGTCCAGAAGAAAGGTTCATTTTCAAACCTCAATTAATTTAGTGACCATCAGCTTCTTTGATTATAGATTAATTGGAAGATTTGTTTGTATATTATTTCTCTCATTTTCAGATACCCACCAATGCGTGATGCCCTAAATTCAACTGGCCGAAACATTTTCTACTCAATTTGTGAATGGTACATTCATCCTCTCTTCCACATATATGATCGAAGTAATGATTTAGGGTTTACTTGGAATGATTTAGGGTTTACTTGGAATGATTTGGCTTCTACCATTTAGTTCAATCTTAGTATGCATGTAATGATTTGGCTTCTACCATTTAGTTCCTACTCTTCCGATGTTCTGGTGAAACATTGGTTTATGTCTATAATGTAATGATTTTCTAGGACACAAATGATTTTTTATTATTATTAAGAAGCTTAATCGTTACTATTTTAGTTATAAAATTGTACAATTAAGAATTGAACCATTGACTTTTTAGATGGTAATTAATATTTTATCTACTAAAGTAGTATGGTGTGTGAGTAGGGGCGTTGATGATCCAGCGTTATGGGCTCCTAGAGTGGGAAACAGTTGGCGTACAACAATTGATATAAGTGATTCATGGGAAAGGTTAGGTTTAAATCTAACCCTAAAATCTTCCTATTATGGCCCTAAATAACCCCTAAAATGTTGCATTTAATGAACTTCAAAAAAAATTGTTTTTTGTTTTGGTTGAAGCATGACTAGAATTGCAGATATCAATGATAAGTGGGCAGAGTATGCAGGACCTGGTGGTTGGAATGGTATGAGATTGATTGTGTTTGAAACCCATTCAACTTCATCCATTTGTTCTCTAAGTTGATCTCTAATTCCCATCTTACCATGTGTGCGTGGACAGATCCAGATATGTTGGAAGTCGGCAATGGTGGCATGACCTATCAAGAATACAGAAGCCATTTTAGCATATGGGCTTTAATGAAGGTTTCAATTTTACAATAATGATAGAATTTGAAAATTTTGTTATGTATACAGCCAAAAGGACAGAAGTTCGAATACTATTTGAATATTAACAAACAAGGAACTCAGGAGTTCATCCAAATGTGCATGCAGGCCCCTCTGTTGATTGGTTGTGATGTGAGAAGCATGTCTCCACAAACTTTGGAAATTCTACGCAATAAGGAAGTCATTGATGTAAACCAAGGTGATCAACATTTATTTATACTATCTTTTCTTATCTTATACTATCTTATACCCTTTTAAACAAGAACACTTAATATTTGCTCACATATCAAAAGGGAAACTTAATTTATGAACGCAGATAGTCTTGGGCTCCAGGGAAGAAAAATTAAAGTTTCTGGCAAAGATGGTTGTCTTCAGGTACCCTTTTTAACTTTTGCTTCATCTTACATTGTATTAAGCATAACAAAGTTATGAATTTAAACTTCAAACTCGTCTTATTTAGTTTACAGTTTTTATTAGATTGTTAAACTTTCTGGTGTTTCTTAGGTGTGGGTTTAGGTGTTTGGTGAACGAGTCAACGAGTGAGGCATGCAAACCAGCAAAAAAAAAAAATAAACCTACACAAGAAACACCAAGAAAAACCTAAACCCATTTAAAACCATTTACCCCACCGTCCTTATAAATCTAGTCAACGTGCATGAGTGTTACACTAATAGAATTAACGACAGATATATATTTTATTACACACAAAATTAAAAATTTCAAACGCCTGACAAAAAACTTATAGTTTAAGGACTATAAGTACATTTATATCGCCTACACTTTGCTAGATTAATATTTTATTTTGGCACATAAGAAGAGAACAGCATGACTTACAATGGCTCCATGAGAAGCAAAGTAAAAAGAAAGAGTTGTGACTTACAGGTGTGGGCAGGTCTCTTATCAAGTTCTGCACCTGAGATTCGCTTGGCTATTGTTCTTTGGAATCGATGTTCAACTGCTTCAATGATCTCCGTAAGCTGGCTTGAACTTGGAGTTATATTTGACATCATTGTCTCAGTAAGGGACTTATGGGAGGTCAGGATCCTAGCTCATCTAAACTAATTTACTCAGTATGCATGAACCGATATTCTCATCTTAGTTAACGACTTCTATGAAGCATCGACACTTTAGTTTAAATGACATGACAATGTGCCTATGTCTTGTGTCTGTGTACGTGCTTCAGTCTTTAGTCTATCTTGGTGCAATGCAGTAGAAATATTAGAGTTGTTTAATCTAACTTGGTCGTCTCATCACCAATACTTGCAGCACAAATTTCTAGATAGCATTAGCCGTGATTCATTTGGGGCTGAAGTTGACTCTCATGACTGCAAGATGTATATCATGACGCATATCCCCTTGTTCCATTCTGAATCTTAGACAATGCTCAGAGACGGGAAGGCCAAGAGATTTAGGAAGTTGTGAAAGAGCTAGATTATGTCATTACTTAAATAAAAAGGGTATCTAGTTTCTCGATCT

mRNA sequence

AATGAGGCAACCGAACACACGGAAAAGCTAGCATTTCATGGCTTTGATAAATCGATTCTTGCATCAAAGTACCTTCAAATTTATAACAACTCCAAATATGGAATTTTGCAGCTCGACAATGGTTTGGGAAAAACTCCTCCCATGGGATGGAATAGTTGGAACCATTTTGGCTGCAGTATAGATGAAAGGGTCATCAAAGAAACTGCGGATGCACTTGTTGCTACGGGACTGGCTGAGTTAGGCTATGTATACGTCAACATAGATGATTGCTGGGCCAACCAACAGAGAATTCTGGTTTTTCTGGCTCCTGATCTCAAAACTTTTCCCTCAGGGATTAAAGCCCTTGCTGATTATGTACATGGGAAAGGCTTGAAGCTTGGAATATATGGTGATGCTGGGCAATTAACTTGCCAGAAGCGACCAGGGTCGCTCGGCTTTGAACTTTGGGATGCAATGATGCTAGCATCTTGGGAGGTGGATTATTTGAAGTATGATAACTGTTATGATATGGGAATCAGTCCAGAAGAAAGATACCCACCAATGCGTGATGCCCTAAATTCAACTGGCCGAAACATTTTCTACTCAATTTGTGAATGGGGCGTTGATGATCCAGCGTTATGGGCTCCTAGAGTGGGAAACAGTTGGCGTACAACAATTGATATAAGTGATTCATGGGAAAGCATGACTAGAATTGCAGATATCAATGATAAGTGGGCAGAGTATGCAGGACCTGGTGGTTGGAATGATCCAGATATGTTGGAAGTCGGCAATGGTGGCATGACCTATCAAGAATACAGAAGCCATTTTAGCATATGGGCTTTAATGAAGGCCCCTCTGTTGATTGGTTGTGATGTGAGAAGCATGTCTCCACAAACTTTGGAAATTCTACGCAATAAGGAAGTCATTGATGTAAACCAAGATAGTCTTGGGCTCCAGGGAAGAAAAATTAAAGTTTCTGGCAAAGATGGTTGTCTTCAGGTGTGGGCAGGTCTCTTATCAAGTTCTGCACCTGAGATTCGCTTGGCTATTGTTCTTTGGAATCGATGTTCAACTGCTTCAATGATCTCCGTAAGCTGGCTTGAACTTGGAGTTATATTTGACATCATTGTCTCAGTAAGGGACTTATGGGAGCACAAATTTCTAGATAGCATTAGCCGTGATTCATTTGGGGCTGAAGTTGACTCTCATGACTGCAAGATGTATATCATGACGCATATCCCCTTGTTCCATTCTGAATCTTAGACAATGCTCAGAGACGGGAAGGCCAAGAGATTTAGGAAGTTGTGAAAGAGCTAGATTATGTCATTACTTAAATAAAAAGGGTATCTAGTTTCTCGATCT

Coding sequence (CDS)

AATGAGGCAACCGAACACACGGAAAAGCTAGCATTTCATGGCTTTGATAAATCGATTCTTGCATCAAAGTACCTTCAAATTTATAACAACTCCAAATATGGAATTTTGCAGCTCGACAATGGTTTGGGAAAAACTCCTCCCATGGGATGGAATAGTTGGAACCATTTTGGCTGCAGTATAGATGAAAGGGTCATCAAAGAAACTGCGGATGCACTTGTTGCTACGGGACTGGCTGAGTTAGGCTATGTATACGTCAACATAGATGATTGCTGGGCCAACCAACAGAGAATTCTGGTTTTTCTGGCTCCTGATCTCAAAACTTTTCCCTCAGGGATTAAAGCCCTTGCTGATTATGTACATGGGAAAGGCTTGAAGCTTGGAATATATGGTGATGCTGGGCAATTAACTTGCCAGAAGCGACCAGGGTCGCTCGGCTTTGAACTTTGGGATGCAATGATGCTAGCATCTTGGGAGGTGGATTATTTGAAGTATGATAACTGTTATGATATGGGAATCAGTCCAGAAGAAAGATACCCACCAATGCGTGATGCCCTAAATTCAACTGGCCGAAACATTTTCTACTCAATTTGTGAATGGGGCGTTGATGATCCAGCGTTATGGGCTCCTAGAGTGGGAAACAGTTGGCGTACAACAATTGATATAAGTGATTCATGGGAAAGCATGACTAGAATTGCAGATATCAATGATAAGTGGGCAGAGTATGCAGGACCTGGTGGTTGGAATGATCCAGATATGTTGGAAGTCGGCAATGGTGGCATGACCTATCAAGAATACAGAAGCCATTTTAGCATATGGGCTTTAATGAAGGCCCCTCTGTTGATTGGTTGTGATGTGAGAAGCATGTCTCCACAAACTTTGGAAATTCTACGCAATAAGGAAGTCATTGATGTAAACCAAGATAGTCTTGGGCTCCAGGGAAGAAAAATTAAAGTTTCTGGCAAAGATGGTTGTCTTCAGGTGTGGGCAGGTCTCTTATCAAGTTCTGCACCTGAGATTCGCTTGGCTATTGTTCTTTGGAATCGATGTTCAACTGCTTCAATGATCTCCGTAAGCTGGCTTGAACTTGGAGTTATATTTGACATCATTGTCTCAGTAAGGGACTTATGGGAGCACAAATTTCTAGATAGCATTAGCCGTGATTCATTTGGGGCTGAAGTTGACTCTCATGACTGCAAGATGTATATCATGACGCATATCCCCTTGTTCCATTCTGAATCTTAG

Protein sequence

NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES

Homology

[Close]

BLAST of Carg00171 vs. NCBI nr
Match: KAG7031516.1 (Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 876.7 bits (2264), Expect = 7.8e-251
Identity = 413/413 (100.00%), Postives = 413/413 (100.00%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH
Sbjct: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 120

Query: 121 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 180
           GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP
Sbjct: 121 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 180

Query: 181 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 240
           MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE
Sbjct: 181 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 240

Query: 241 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE 300
           YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE
Sbjct: 241 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE 300

Query: 301 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 360
           VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL
Sbjct: 301 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 360

Query: 361 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 361 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 413

[Close]

BLAST of Carg00171 vs. NCBI nr
Match: KAG6600882.1 (Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 873.6 bits (2256), Expect = 6.6e-250
Identity = 412/413 (99.76%), Postives = 412/413 (99.76%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 48  NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 107

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH
Sbjct: 108 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 167

Query: 121 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 180
           GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP
Sbjct: 168 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 227

Query: 181 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 240
           MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSW TTIDISDSWESMTRIADINDKWAE
Sbjct: 228 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWCTTIDISDSWESMTRIADINDKWAE 287

Query: 241 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE 300
           YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE
Sbjct: 288 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE 347

Query: 301 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 360
           VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL
Sbjct: 348 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 407

Query: 361 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 408 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 460

[Close]

BLAST of Carg00171 vs. NCBI nr
Match: XP_022943112.1 (alpha-galactosidase 3-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 855.9 bits (2210), Expect = 1.4e-244
Identity = 405/413 (98.06%), Postives = 406/413 (98.31%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGF KS     YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 68  NEATEHTEKLAFHGFHKS-----YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 127

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH
Sbjct: 128 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 187

Query: 121 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 180
           GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP
Sbjct: 188 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 247

Query: 181 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 240
           MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE
Sbjct: 248 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 307

Query: 241 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE 300
           YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL NKE
Sbjct: 308 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKE 367

Query: 301 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 360
           VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL
Sbjct: 368 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 427

Query: 361 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 428 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 475

[Close]

BLAST of Carg00171 vs. NCBI nr
Match: XP_022943111.1 (alpha-galactosidase 3-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 850.9 bits (2197), Expect = 4.6e-243
Identity = 405/415 (97.59%), Postives = 406/415 (97.83%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGF KS     YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 68  NEATEHTEKLAFHGFHKS-----YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 127

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRIL--VFLAPDLKTFPSGIKALADY 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRIL  VFLAPDLKTFPSGIKALADY
Sbjct: 128 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVIVFLAPDLKTFPSGIKALADY 187

Query: 121 VHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERY 180
           VHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERY
Sbjct: 188 VHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERY 247

Query: 181 PPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKW 240
           PPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKW
Sbjct: 248 PPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKW 307

Query: 241 AEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRN 300
           AEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL N
Sbjct: 308 AEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSN 367

Query: 301 KEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVS 360
           KEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVS
Sbjct: 368 KEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVS 427

Query: 361 WLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           WLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 428 WLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 477

[Close]

BLAST of Carg00171 vs. NCBI nr
Match: XP_022943110.1 (alpha-galactosidase 3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 849.4 bits (2193), Expect = 1.3e-242
Identity = 404/416 (97.12%), Postives = 406/416 (97.60%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGF KS     YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 68  NEATEHTEKLAFHGFHKS-----YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 127

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILV---FLAPDLKTFPSGIKALAD 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILV   +LAPDLKTFPSGIKALAD
Sbjct: 128 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVISPYLAPDLKTFPSGIKALAD 187

Query: 121 YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER 180
           YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER
Sbjct: 188 YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER 247

Query: 181 YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK 240
           YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK
Sbjct: 248 YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK 307

Query: 241 WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILR 300
           WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL 
Sbjct: 308 WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILS 367

Query: 301 NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV 360
           NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV
Sbjct: 368 NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV 427

Query: 361 SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 428 SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 478

[Close]

BLAST of Carg00171 vs. ExPASy Swiss-Prot
Match: Q8VXZ7 (Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 2.1e-158
Identity = 257/395 (65.06%), Postives = 312/395 (78.99%), Query Frame = 0


Query: 19  ILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLA 78
           + +  +  IY+ S YG LQL+NGL +TP MGWNSWN F C+I+E VIKETADALV++GLA
Sbjct: 46  VFSKSFNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLA 105

Query: 79  ELGYVYVNIDDCWANQQRILV-FLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTC 138
           +LGY++VNIDDCW+N  R     L P  +TFPSGIK LADYVH KGLKLGIY DAG  TC
Sbjct: 106 DLGYIHVNIDDCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC 165

Query: 139 QKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSIC 198
           +  PGSL  E+ DA + ASW VDYLKYDNC+++GI P ERYPPMRDALN+TGR+IFYS+C
Sbjct: 166 EVHPGSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLC 225

Query: 199 EWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGN 258
           EWGVDDPALWA  VGNSWRTT DI+D+W SMT IAD+N+KWA YAGPGGWNDPDMLE+GN
Sbjct: 226 EWGVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGN 285

Query: 259 GGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIK 318
           GGMTY+EYR HFSIWALMKAPLLIGCDVR+M+ +TLEIL NKE+I VNQD LG+QGRKI+
Sbjct: 286 GGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQ 345

Query: 319 VSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWE 378
            +G++ C QVW+G LS      R+ + LWNRCS  + I+ SW  +G+   I VSVRDLW+
Sbjct: 346 ANGENDCQQVWSGPLSGD----RMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQ 405

Query: 379 HKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSE 413
           HK +   +  SF A+VD+HDC MY++T   + HS+
Sbjct: 406 HKDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSD 436

[Close]

BLAST of Carg00171 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 7.6e-140
Identity = 234/391 (59.85%), Postives = 286/391 (73.15%), Query Frame = 0


Query: 16  DKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVAT 75
           +K    S   + YN  +Y     +NGLG+TPPMGWNSWNHFGC I+E V++ETADA+V+T
Sbjct: 29  EKKNRTSAEAEHYNVRRY---LAENGLGQTPPMGWNSWNHFGCDINENVVRETADAMVST 88

Query: 76  GLAELGYVYVNIDDCWANQQRILV-FLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQ 135
           GLA LGY Y+N+DDCWA   R     + P+   FPSGIKALADYVH KGLKLG+Y DAG 
Sbjct: 89  GLAALGYQYINLDDCWAELNRDSEGNMVPNAAAFPSGIKALADYVHSKGLKLGVYSDAGN 148

Query: 136 LTCQKR-PGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIF 195
            TC KR PGSLG E  DA   ASW VDYLKYDNC ++GIS +ERYPPM  AL S+GR IF
Sbjct: 149 QTCSKRMPGSLGHEEQDAKTFASWGVDYLKYDNCENLGISVKERYPPMGKALLSSGRPIF 208

Query: 196 YSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDML 255
           +S+CEWG +DP +WA  +GNSWRTT DI D+W SMT IAD NDKWA YAGPGGWNDPDML
Sbjct: 209 FSMCEWGWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAGPGGWNDPDML 268

Query: 256 EVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQG 315
           EVGNGGMT +EYRSHFSIWAL KAPLL+GCD+R+M   T E++ N EVI VNQD LG+QG
Sbjct: 269 EVGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTTHELISNAEVIAVNQDKLGVQG 328

Query: 316 RKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVR 375
           +K+K +     L+VWAG LS +    ++A++LWNR S+ + ++ SW ++G+     V  R
Sbjct: 329 KKVKSTND---LEVWAGPLSDN----KVAVILWNRSSSRATVTASWSDIGLQQGTTVDAR 388

Query: 376 DLWEHKFLDSISRDSFGAEVDSHDCKMYIMT 405
           DLWEH    S+      AE+DSH CKMY++T
Sbjct: 389 DLWEHS-TQSLVSGEISAEIDSHACKMYVLT 408

[Close]

BLAST of Carg00171 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.5e-135
Identity = 232/369 (62.87%), Postives = 271/369 (73.44%), Query Frame = 0


Query: 38  LDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQR- 97
           L NGLG TPPMGWNSWNHF C++DE++I+ETADA+V+ GLA LGY Y+N+DDCWA   R 
Sbjct: 16  LANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWAELNRD 75

Query: 98  ILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTCQK-RPGSLGFELWDAMMLA 157
               L P   TFPSGIKALADYVH KGLKLGIY DAG  TC K  PGSLG E  DA   A
Sbjct: 76  SQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQDAKTFA 135

Query: 158 SWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSW 217
           SW VDYLKYDNC +  ISP+ERYP M  AL ++GR+IF+S+CEWG +DPA WA  VGNSW
Sbjct: 136 SWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKEVGNSW 195

Query: 218 RTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALM 277
           RTT DI DSW SMT  AD+NDKWA YAGPGGWNDPDMLEVGNGGMT  EYRSHFSIWAL 
Sbjct: 196 RTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSIWALA 255

Query: 278 KAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSS 337
           KAPLLIGCD+RSM   T ++L N EVI VNQD LG+QG K+K  G    L+VWAG LS  
Sbjct: 256 KAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGD---LEVWAGPLSGK 315

Query: 338 APEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDS 397
               R+A+ LWNR S+ + I+  W ++G+    +V+ RDLW H    S+ +    A VD+
Sbjct: 316 ----RVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSV-KGQISAAVDA 375

Query: 398 HDCKMYIMT 405
           HD KMY++T
Sbjct: 376 HDSKMYVLT 376

[Close]

BLAST of Carg00171 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 3.4e-132
Identity = 229/368 (62.23%), Postives = 269/368 (73.10%), Query Frame = 0


Query: 39  DNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQR-I 98
           +NGLG+TP MGWNSWNHF C I+E++I+ETADALV TGLA+LGY YVNIDDCWA   R  
Sbjct: 57  ENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAEYSRDS 116

Query: 99  LVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTC-QKRPGSLGFELWDAMMLAS 158
                P+ +TFPSGIKALADYVH KGLKLGIY DAG  TC  K PGSL  E  D    AS
Sbjct: 117 QGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDVKTFAS 176

Query: 159 WEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWR 218
           W VDYLKYDNC D G S  ERY  M +A+ + G+NIF+S+CEWG ++PA WA R+GNSWR
Sbjct: 177 WGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRMGNSWR 236

Query: 219 TTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMK 278
           TT DI+D+W SMT  AD ND+WA YAGPGGWNDPDMLEVGNGGM+  EYRSHFSIWAL K
Sbjct: 237 TTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAK 296

Query: 279 APLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSA 338
           APLLIGCDVRSMS QT  IL N EVI VNQDSLG+QG+K++    D  L+VWAG LS++ 
Sbjct: 297 APLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQ---SDNGLEVWAGPLSNN- 356

Query: 339 PEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSH 398
              R A+VLWNR S  + I+  W  +G+   + V+ RDLW H      ++    A V  H
Sbjct: 357 ---RKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHS--SFAAQGQISASVAPH 415

Query: 399 DCKMYIMT 405
           DCKMY++T
Sbjct: 417 DCKMYVLT 415

[Close]

BLAST of Carg00171 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 5.8e-132
Identity = 223/369 (60.43%), Postives = 271/369 (73.44%), Query Frame = 0


Query: 38  LDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQR- 97
           ++NGL  +P MGWNSWNHF C+I+E +IK+TADA+V++GL+ +GY Y+NIDDCW   +R 
Sbjct: 32  MNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGELKRD 91

Query: 98  ILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTC-QKRPGSLGFELWDAMMLA 157
               L     TFPSGIKAL+DYVH KGLKLGIY DAG LTC Q  PGSLG E  DA   A
Sbjct: 92  SQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFA 151

Query: 158 SWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSW 217
           SW +DYLKYDNC + G SP ERYP M  AL ++GR+IF+S+CEWG +DPA WA  +GNSW
Sbjct: 152 SWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIGNSW 211

Query: 218 RTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALM 277
           RTT DI D+W+SMT IAD ND+WA YA PG WNDPDMLEVGNGGMT +EY SHFSIWAL 
Sbjct: 212 RTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALA 271

Query: 278 KAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSS 337
           KAPLLIGCD+RSM   T E+L NKEVI VNQD LG+QG+K+K   K+G L+VWAG LS  
Sbjct: 272 KAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKK 331

Query: 338 APEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDS 397
               R+A++LWNR S ++ I+  W E+G+    IV+ RDLWEH     + +    A V+ 
Sbjct: 332 ----RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKK-QLSALVEP 391

Query: 398 HDCKMYIMT 405
           H CKMY +T
Sbjct: 392 HACKMYTLT 392

[Close]

BLAST of Carg00171 vs. ExPASy TrEMBL
Match: A0A6J1FS41 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1)

HSP 1 Score: 855.9 bits (2210), Expect = 6.9e-245
Identity = 405/413 (98.06%), Postives = 406/413 (98.31%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGF KS     YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 68  NEATEHTEKLAFHGFHKS-----YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 127

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH
Sbjct: 128 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVH 187

Query: 121 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 180
           GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP
Sbjct: 188 GKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPP 247

Query: 181 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 240
           MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE
Sbjct: 248 MRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAE 307

Query: 241 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKE 300
           YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL NKE
Sbjct: 308 YAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKE 367

Query: 301 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 360
           VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL
Sbjct: 368 VIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWL 427

Query: 361 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 428 ELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 475

[Close]

BLAST of Carg00171 vs. ExPASy TrEMBL
Match: A0A6J1FQU0 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1)

HSP 1 Score: 850.9 bits (2197), Expect = 2.2e-243
Identity = 405/415 (97.59%), Postives = 406/415 (97.83%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGF KS     YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 68  NEATEHTEKLAFHGFHKS-----YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 127

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRIL--VFLAPDLKTFPSGIKALADY 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRIL  VFLAPDLKTFPSGIKALADY
Sbjct: 128 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVIVFLAPDLKTFPSGIKALADY 187

Query: 121 VHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERY 180
           VHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERY
Sbjct: 188 VHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERY 247

Query: 181 PPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKW 240
           PPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKW
Sbjct: 248 PPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKW 307

Query: 241 AEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRN 300
           AEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL N
Sbjct: 308 AEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSN 367

Query: 301 KEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVS 360
           KEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVS
Sbjct: 368 KEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVS 427

Query: 361 WLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           WLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 428 WLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 477

[Close]

BLAST of Carg00171 vs. ExPASy TrEMBL
Match: A0A6J1FXE1 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 6.5e-243
Identity = 404/416 (97.12%), Postives = 406/416 (97.60%), Query Frame = 0


Query: 1   NEATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 60
           NEATEHTEKLAFHGF KS     YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI
Sbjct: 68  NEATEHTEKLAFHGFHKS-----YLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSI 127

Query: 61  DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILV---FLAPDLKTFPSGIKALAD 120
           DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILV   +LAPDLKTFPSGIKALAD
Sbjct: 128 DERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVISPYLAPDLKTFPSGIKALAD 187

Query: 121 YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER 180
           YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER
Sbjct: 188 YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER 247

Query: 181 YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK 240
           YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK
Sbjct: 248 YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK 307

Query: 241 WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILR 300
           WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL 
Sbjct: 308 WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILS 367

Query: 301 NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV 360
           NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV
Sbjct: 368 NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV 427

Query: 361 SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 428 SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 478

[Close]

BLAST of Carg00171 vs. ExPASy TrEMBL
Match: A0A6J1JG86 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111484225 PE=3 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 1.7e-238
Identity = 394/416 (94.71%), Postives = 404/416 (97.12%), Query Frame = 0


Query: 2   EATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSID 61
           EATEHTEKLAFHGFDKSILASKYLQIYNNSKYG LQLDNGLGKTPPMGWNSWNHFGCS+D
Sbjct: 49  EATEHTEKLAFHGFDKSILASKYLQIYNNSKYGTLQLDNGLGKTPPMGWNSWNHFGCSVD 108

Query: 62  ERVIKETADALVATGLAELGYVYVNIDDCWANQQRILVF----LAPDLKTFPSGIKALAD 121
           ERVIKETADALVATGLAELGYVYVNIDDCWAN++RILVF    LAPD  TFPSGIKALAD
Sbjct: 109 ERVIKETADALVATGLAELGYVYVNIDDCWANKRRILVFVAPLLAPDPNTFPSGIKALAD 168

Query: 122 YVHGKGLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEER 181
           YVHGKGLKLGIYGDAGQLTCQ+RPGSLGFEL DAM+LASWEVDYLKYDNCYDMGISP++R
Sbjct: 169 YVHGKGLKLGIYGDAGQLTCQQRPGSLGFELLDAMVLASWEVDYLKYDNCYDMGISPQKR 228

Query: 182 YPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK 241
           YP MRDALNST R+IFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK
Sbjct: 229 YPAMRDALNSTARSIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDK 288

Query: 242 WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILR 301
           WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL 
Sbjct: 289 WAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILS 348

Query: 302 NKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV 361
           NKEVIDVNQDSLGLQGRKI VSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV
Sbjct: 349 NKEVIDVNQDSLGLQGRKINVSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISV 408

Query: 362 SWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 414
           SWLELGVIFD+IVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES
Sbjct: 409 SWLELGVIFDVIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSES 464

[Close]

BLAST of Carg00171 vs. ExPASy TrEMBL
Match: A0A6J1CZ60 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111015929 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.9e-202
Identity = 330/408 (80.88%), Postives = 361/408 (88.48%), Query Frame = 0


Query: 3   ATEHTEKLAFHGFDKSILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSIDE 62
           A+E T +L  HGFD SI  SK+ QI+NNSKYGILQ DNGLGKTPPMGWNSWNHFGC +D 
Sbjct: 49  ASEMTVELPLHGFDSSISTSKFHQIFNNSKYGILQFDNGLGKTPPMGWNSWNHFGCYVDA 108

Query: 63  RVIKETADALVATGLAELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVHGK 122
            VIKETADALV++GLAELGYVYVNIDDCWA ++R+L  + PD  +FP GIKALADYVHGK
Sbjct: 109 TVIKETADALVSSGLAELGYVYVNIDDCWAYKERVLGLMIPDANSFPQGIKALADYVHGK 168

Query: 123 GLKLGIYGDAGQLTCQKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPPMR 182
           GLKLGIY DAG+LTCQ+RPGS G E  DAMM A W VDYLKYDNCYDMGI P++RYPPMR
Sbjct: 169 GLKLGIYSDAGELTCQRRPGSYGQEFRDAMMFADWGVDYLKYDNCYDMGIEPQKRYPPMR 228

Query: 183 DALNSTGRNIFYSICEWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYA 242
           DALNSTGR+IFYSICEWGVDDPALWAP++GNSWRTTIDISDSW+SMT IADINDKWA YA
Sbjct: 229 DALNSTGRSIFYSICEWGVDDPALWAPKIGNSWRTTIDISDSWDSMTSIADINDKWAAYA 288

Query: 243 GPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKEVI 302
           GPGGWNDPDMLEVGNGGMTY EYRSHFSIWALMKAPLLIGCDVR+MSPQTLEIL NK+VI
Sbjct: 289 GPGGWNDPDMLEVGNGGMTYNEYRSHFSIWALMKAPLLIGCDVRNMSPQTLEILSNKQVI 348

Query: 303 DVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSS-APEIRLAIVLWNRCSTASMISVSWLE 362
           +VNQD LG+QGRKIKVSGKDGCLQVWAG LS     E+RLA+VLWNRCS ASMIS++WLE
Sbjct: 349 EVNQDRLGIQGRKIKVSGKDGCLQVWAGPLSGQYGSEMRLAVVLWNRCSYASMISITWLE 408

Query: 363 LGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDSHDCKMYIMTHIPLF 410
           LG I D I SVRDLWEHKF+  I RDSFGAEVDSHDCKMYI+T+IPLF
Sbjct: 409 LGFILDYIFSVRDLWEHKFV-LIGRDSFGAEVDSHDCKMYIITYIPLF 455

[Close]

BLAST of Carg00171 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 560.1 bits (1442), Expect = 1.5e-159
Identity = 257/395 (65.06%), Postives = 312/395 (78.99%), Query Frame = 0


Query: 19  ILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLA 78
           + +  +  IY+ S YG LQL+NGL +TP MGWNSWN F C+I+E VIKETADALV++GLA
Sbjct: 46  VFSKSFNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLA 105

Query: 79  ELGYVYVNIDDCWANQQRILV-FLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTC 138
           +LGY++VNIDDCW+N  R     L P  +TFPSGIK LADYVH KGLKLGIY DAG  TC
Sbjct: 106 DLGYIHVNIDDCWSNLLRDSEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC 165

Query: 139 QKRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSIC 198
           +  PGSL  E+ DA + ASW VDYLKYDNC+++GI P ERYPPMRDALN+TGR+IFYS+C
Sbjct: 166 EVHPGSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLC 225

Query: 199 EWGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGN 258
           EWGVDDPALWA  VGNSWRTT DI+D+W SMT IAD+N+KWA YAGPGGWNDPDMLE+GN
Sbjct: 226 EWGVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGN 285

Query: 259 GGMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIK 318
           GGMTY+EYR HFSIWALMKAPLLIGCDVR+M+ +TLEIL NKE+I VNQD LG+QGRKI+
Sbjct: 286 GGMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQ 345

Query: 319 VSGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWE 378
            +G++ C QVW+G LS      R+ + LWNRCS  + I+ SW  +G+   I VSVRDLW+
Sbjct: 346 ANGENDCQQVWSGPLSGD----RMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQ 405

Query: 379 HKFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSE 413
           HK +   +  SF A+VD+HDC MY++T   + HS+
Sbjct: 406 HKDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSD 436

[Close]

BLAST of Carg00171 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.4e-149
Identity = 245/394 (62.18%), Postives = 298/394 (75.63%), Query Frame = 0


Query: 19  ILASKYLQIYNNSKYGILQLDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLA 78
           + +  +  IY+ S YG LQL+NGL +TP MGWNSWN F C+I+E VIKETADALV++GLA
Sbjct: 46  VFSKSFNSIYDTSMYGRLQLNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLA 105

Query: 79  ELGYVYVNIDDCWANQQRILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTCQ 138
           +LGY++VNI                       GIK LADYVH KGLKLGIY DAG  TC+
Sbjct: 106 DLGYIHVNI-----------------------GIKLLADYVHSKGLKLGIYSDAGVFTCE 165

Query: 139 KRPGSLGFELWDAMMLASWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICE 198
             PGSL  E+ DA + ASW VDYLKYDNC+++GI P ERYPPMRDALN+TGR+IFYS+CE
Sbjct: 166 VHPGSLFHEVDDADIFASWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCE 225

Query: 199 WGVDDPALWAPRVGNSWRTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNG 258
           WGVDDPALWA  VGNSWRTT DI+D+W SMT IAD+N+KWA YAGPGGWNDPDMLE+GNG
Sbjct: 226 WGVDDPALWAKEVGNSWRTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNG 285

Query: 259 GMTYQEYRSHFSIWALMKAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKV 318
           GMTY+EYR HFSIWALMKAPLLIGCDVR+M+ +TLEIL NKE+I VNQD LG+QGRKI+ 
Sbjct: 286 GMTYEEYRGHFSIWALMKAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQA 345

Query: 319 SGKDGCLQVWAGLLSSSAPEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEH 378
           +G++ C QVW+G LS      R+ + LWNRCS  + I+ SW  +G+   I VSVRDLW+H
Sbjct: 346 NGENDCQQVWSGPLSGD----RMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQH 405

Query: 379 KFLDSISRDSFGAEVDSHDCKMYIMTHIPLFHSE 413
           K +   +  SF A+VD+HDC MY++T   + HS+
Sbjct: 406 KDVTENTSGSFEAQVDAHDCHMYVLTPQTVSHSD 412

[Close]

BLAST of Carg00171 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 472.2 bits (1214), Expect = 4.2e-133
Identity = 223/369 (60.43%), Postives = 271/369 (73.44%), Query Frame = 0


Query: 38  LDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQR- 97
           ++NGL  +P MGWNSWNHF C+I+E +IK+TADA+V++GL+ +GY Y+NIDDCW   +R 
Sbjct: 32  MNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGELKRD 91

Query: 98  ILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTC-QKRPGSLGFELWDAMMLA 157
               L     TFPSGIKAL+DYVH KGLKLGIY DAG LTC Q  PGSLG E  DA   A
Sbjct: 92  SQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFA 151

Query: 158 SWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSW 217
           SW +DYLKYDNC + G SP ERYP M  AL ++GR+IF+S+CEWG +DPA WA  +GNSW
Sbjct: 152 SWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIGNSW 211

Query: 218 RTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALM 277
           RTT DI D+W+SMT IAD ND+WA YA PG WNDPDMLEVGNGGMT +EY SHFSIWAL 
Sbjct: 212 RTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALA 271

Query: 278 KAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSS 337
           KAPLLIGCD+RSM   T E+L NKEVI VNQD LG+QG+K+K   K+G L+VWAG LS  
Sbjct: 272 KAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKK 331

Query: 338 APEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDS 397
               R+A++LWNR S ++ I+  W E+G+    IV+ RDLWEH     + +    A V+ 
Sbjct: 332 ----RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKK-QLSALVEP 391

Query: 398 HDCKMYIMT 405
           H CKMY +T
Sbjct: 392 HACKMYTLT 392

[Close]

BLAST of Carg00171 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 472.2 bits (1214), Expect = 4.2e-133
Identity = 223/369 (60.43%), Postives = 271/369 (73.44%), Query Frame = 0


Query: 38  LDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQR- 97
           ++NGL  +P MGWNSWNHF C+I+E +IK+TADA+V++GL+ +GY Y+NIDDCW   +R 
Sbjct: 6   MNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWGELKRD 65

Query: 98  ILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTC-QKRPGSLGFELWDAMMLA 157
               L     TFPSGIKAL+DYVH KGLKLGIY DAG LTC Q  PGSLG E  DA   A
Sbjct: 66  SQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFA 125

Query: 158 SWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSW 217
           SW +DYLKYDNC + G SP ERYP M  AL ++GR+IF+S+CEWG +DPA WA  +GNSW
Sbjct: 126 SWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGDIGNSW 185

Query: 218 RTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALM 277
           RTT DI D+W+SMT IAD ND+WA YA PG WNDPDMLEVGNGGMT +EY SHFSIWAL 
Sbjct: 186 RTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFSIWALA 245

Query: 278 KAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSS 337
           KAPLLIGCD+RSM   T E+L NKEVI VNQD LG+QG+K+K   K+G L+VWAG LS  
Sbjct: 246 KAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKK 305

Query: 338 APEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDS 397
               R+A++LWNR S ++ I+  W E+G+    IV+ RDLWEH     + +    A V+ 
Sbjct: 306 ----RVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCVKK-QLSALVEP 365

Query: 398 HDCKMYIMT 405
           H CKMY +T
Sbjct: 366 HACKMYTLT 366

[Close]

BLAST of Carg00171 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 450.7 bits (1158), Expect = 1.3e-126
Identity = 217/368 (58.97%), Postives = 264/368 (71.74%), Query Frame = 0


Query: 38  LDNGLGKTPPMGWNSWNHFGCSIDERVIKETADALVATGLAELGYVYVNIDDCWANQQR- 97
           L NGLG TPPMGWNSWNHF C+IDE++IKETADALV TGL++LGY YVNIDDCWA   R 
Sbjct: 46  LTNGLGVTPPMGWNSWNHFSCNIDEKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRD 105

Query: 98  ILVFLAPDLKTFPSGIKALADYVHGKGLKLGIYGDAGQLTCQK-RPGSLGFELWDAMMLA 157
               L P   TFPSGIKA+ADYVH KGLKLGIY DAG  TC K  PGSLG+E  DA   A
Sbjct: 106 SKGSLVPKKSTFPSGIKAVADYVHSKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFA 165

Query: 158 SWEVDYLKYDNCYDMGISPEERYPPMRDALNSTGRNIFYSICEWGVDDPALWAPRVGNSW 217
            W +DYLKYDNC   G  P  RYP M  AL  +GR IF+S+CEWG   PALW   VGNSW
Sbjct: 166 EWGIDYLKYDNCNSDGSKPTVRYPVMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSW 225

Query: 218 RTTIDISDSWESMTRIADINDKWAEYAGPGGWNDPDMLEVGNGGMTYQEYRSHFSIWALM 277
           RTT DI D+W SM  IAD+N+ +AE+A PGGWNDPDMLEVGNGGMT  EY  HFSIWA+ 
Sbjct: 226 RTTNDIKDTWLSMISIADMNEVYAEHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAIS 285

Query: 278 KAPLLIGCDVRSMSPQTLEILRNKEVIDVNQDSLGLQGRKIKVSGKDGCLQVWAGLLSSS 337
           KAPLL+GCD+R+M+ +T+EI+ NKEVI +NQD  G+Q +K+++   +G L+VWAG LS  
Sbjct: 286 KAPLLLGCDIRNMTKETMEIVANKEVIAINQDPHGVQAKKVRM---EGDLEVWAGPLSG- 345

Query: 338 APEIRLAIVLWNRCSTASMISVSWLELGVIFDIIVSVRDLWEHKFLDSISRDSFGAEVDS 397
               R+A++L NR  + + I+  W ++ +  + IV  RDLWEH+ L      +  A VDS
Sbjct: 346 ---YRVALLLLNRGPSRTSITALWEDIEIPANSIVEARDLWEHQTLKQKFVGNLTATVDS 405

Query: 398 HDCKMYIM 404
           H CK+Y++
Sbjct: 406 HACKLYVL 406

The following BLAST results are available for this feature:

BLAST of Carg00171 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031516.1	7.8e-251	100.00	Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6600882.1	6.6e-250	99.76	Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022943112.1	1.4e-244	98.06	alpha-galactosidase 3-like isoform X3 [Cucurbita moschata]	[more]
XP_022943111.1	4.6e-243	97.59	alpha-galactosidase 3-like isoform X2 [Cucurbita moschata]	[more]
XP_022943110.1	1.3e-242	97.12	alpha-galactosidase 3-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg00171 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VXZ7	2.1e-158	65.06	Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1	[more]
P14749	7.6e-140	59.85	Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1	[more]
Q42656	1.5e-135	62.87	Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1	[more]
Q9FXT4	3.4e-132	62.23	Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=...	[more]
Q8RX86	5.8e-132	60.43	Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1	[more]

BLAST of Carg00171 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FS41	6.9e-245	98.06	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1	[more]
A0A6J1FQU0	2.2e-243	97.59	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1	[more]
A0A6J1FXE1	6.5e-243	97.12	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447939 PE=3 SV=1	[more]
A0A6J1JG86	1.7e-238	94.71	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111484225 PE=3 SV=1	[more]
A0A6J1CZ60	1.9e-202	80.88	Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111015929 PE=3 SV=1	[more]

BLAST of Carg00171 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56310.1	1.5e-159	65.06	Melibiase family protein	[more]
AT3G56310.2	1.4e-149	62.18	Melibiase family protein	[more]
AT5G08370.1	4.2e-133	60.43	alpha-galactosidase 2	[more]
AT5G08370.2	4.2e-133	60.43	alpha-galactosidase 2	[more]
AT5G08380.1	1.3e-126	58.97	alpha-galactosidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002241	Glycoside hydrolase, family 27	PRINTS	PR00740	GLHYDRLASE27	coord: 177..195 score: 61.32 coord: 242..261 score: 73.75 coord: 263..284 score: 55.68 coord: 40..59 score: 66.5 coord: 150..167 score: 66.67 coord: 116..137 score: 58.41 coord: 76..91 score: 66.88
IPR002241	Glycoside hydrolase, family 27	PFAM	PF16499	Melibiase_2	coord: 45..307 e-value: 3.3E-78 score: 262.7
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 28..405
IPR002241	Glycoside hydrolase, family 27	CDD	cd14792	GH27	coord: 46..307 e-value: 5.39749E-149 score: 421.579
IPR041233	Alpha galactosidase, C-terminal beta sandwich domain	PFAM	PF17801	Melibiase_C	coord: 322..402 e-value: 5.9E-11 score: 42.3
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 38..309 e-value: 3.6E-126 score: 422.3
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 310..405 e-value: 7.6E-19 score: 69.5
None	No IPR available	PANTHER	PTHR11452:SF36	ALPHA-GALACTOSIDASE	coord: 28..405
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 320..404
IPR000111	Glycoside hydrolase family 27/36, conserved site	PROSITE	PS00512	ALPHA_GALACTOSIDASE	coord: 81..100
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 37..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00171-RA	Carg00171-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0009505	plant-type cell wall
molecular_function	GO:0052692	raffinose alpha-galactosidase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds