Carg00144 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00144
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr04: 5584879 .. 5586541 (+)
RNA-Seq ExpressionCarg00144
SyntenyCarg00144
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGAGGTGGCGGCGGAGGCGGCGGCGGCAGCATGGCGGAGCTGACGATCCCGAGCCATTTCCTGTGCCCAATATCGCTGGACTTGATGAAAGATCCAGTCACCATGTCCACCGGCATCACCTACGATCGAGCCAACATTGAGACATGGATTGAAGGAGGCAATTTCAGCTGCCCCTTCACTAACCAACCTTTGCACACCATTGATTCCATTCCTAATCACAACATAAGGAAGATGATCCAAGCTTGGTGCGTCGAGAATAGGACTCATGGGATCGAGAGGATTCCGACGCCGAGGGTTCCCGCCAGCCCCGTGCAGGTTCGGGAGATTCTTGACAAGATCGCTGCTGCGGTCGGGCGGGAGGATTGTGAGGGATGCAAGTCCTTGGTTGAGAAGATCAAGATATTGGTACAAGAGAGTGAAAGGAACAAGAAATGCATTATTGCCAATGGAACAGGTTCGTAAACTACTTCATAGCTAACATCCCACTTCAGGTGGGGAGGAAAACGAAATACCCTTTGTTACAAGGGTGTGTAAACCTTTCCTTAACCGACCCATTTTAAAATTATCGAGGAAAAGCTCAAATAGGACAATATCTGCTAGTGGTTGCCTTCGACTCTTACAAATGAGCGCCCACTTTGGTTGAGGAGTATAACGAAACACCCTTTGTTACAAGGGTCTGTAAATCTCTCCATAACCGACCCATTTTAAAATTATTAAGGAAAATCTCAAATAGGACTCTTACAAATTAGCTACGAGATTCAAAGGTCGAACCTTTTTTATCGTTATTTTTGTTTATATGTTAACAGGAAATGTATTATCCTCCGCATTCGAAGCATTTTCGAAACCCGAACGATTCGAGAAAAGGGTCGAGATTTTAGAAGAAATCTTATCGACGATGGCAATGATCTTCCCTCTACAAGAAGAAGCCATTAAACATCTAAAATCAGAAGCTTCAATGCAAGCATTGGTGTGGTTTGTAAGAGGAGAAGACAAATCAAGAAGAAGAAGCTCAATTTTGGTATTGAAAGAGATAATTTCATCATACCCTGAAAAAGTAAGCGAATTAGGAGAAATCCAAGGAGTATTAGAGGGTCTTATGAAGCTCATAAAAGAACCCATTTGTTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGCCATAGCCTCTTCTTCCAATCAAGAGGGCTTCACAAACGCATTTCTTCAAATGGGGTTGGTCCCTTTGCTACTCGAAACCCTCGTAGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGTGTTCGATGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCATGCTTTGACGATGCCCGTGATTGTGAAAAAGATACTTAGAGTGTCGGATTTGGCAACCGAGCTATCGGTGTCGATCGTTTGGAAGCTCGTAAAGCACGAGAGTAGAGAAGAGGAAGATGAAGATGAAGATGGAGGAGTTAAAGTAGAAGCTCTTCAAGTTGGAGCATTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGAAAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACCTTTTGA

mRNA sequence

ATGGCTAGAGGTGGCGGCGGAGGCGGCGGCGGCAGCATGGCGGAGCTGACGATCCCGAGCCATTTCCTGTGCCCAATATCGCTGGACTTGATGAAAGATCCAGTCACCATGTCCACCGGCATCACCTACGATCGAGCCAACATTGAGACATGGATTGAAGGAGGCAATTTCAGCTGCCCCTTCACTAACCAACCTTTGCACACCATTGATTCCATTCCTAATCACAACATAAGGAAGATGATCCAAGCTTGGTGCGTCGAGAATAGGACTCATGGGATCGAGAGGATTCCGACGCCGAGGGTTCCCGCCAGCCCCGTGCAGGTTCGGGAGATTCTTGACAAGATCGCTGCTGCGGTCGGGCGGGAGGATTGTGAGGGATGCAAGTCCTTGGTTGAGAAGATCAAGATATTGGTACAAGAGAGTGAAAGGAACAAGAAATGCATTATTGCCAATGGAACAGGAAATGTATTATCCTCCGCATTCGAAGCATTTTCGAAACCCGAACGATTCGAGAAAAGGGTCGAGATTTTAGAAGAAATCTTATCGACGATGGCAATGATCTTCCCTCTACAAGAAGAAGCCATTAAACATCTAAAATCAGAAGCTTCAATGCAAGCATTGGTGTGGTTTGTAAGAGGAGAAGACAAATCAAGAAGAAGAAGCTCAATTTTGGTATTGAAAGAGATAATTTCATCATACCCTGAAAAAGTAAGCGAATTAGGAGAAATCCAAGGAGTATTAGAGGGTCTTATGAAGCTCATAAAAGAACCCATTTGTTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGCCATAGCCTCTTCTTCCAATCAAGAGGGCTTCACAAACGCATTTCTTCAAATGGGGTTGGTCCCTTTGCTACTCGAAACCCTCGTAGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGTGTTCGATGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCATGCTTTGACGATGCCCGTGATTGTGAAAAAGATACTTAGAGTGTCGGATTTGGCAACCGAGCTATCGGTGTCGATCGTTTGGAAGCTCGTAAAGCACGAGAGTAGAGAAGAGGAAGATGAAGATGAAGATGGAGGAGTTAAAGTAGAAGCTCTTCAAGTTGGAGCATTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGAAAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACCTTTTGA

Coding sequence (CDS)

ATGGCTAGAGGTGGCGGCGGAGGCGGCGGCGGCAGCATGGCGGAGCTGACGATCCCGAGCCATTTCCTGTGCCCAATATCGCTGGACTTGATGAAAGATCCAGTCACCATGTCCACCGGCATCACCTACGATCGAGCCAACATTGAGACATGGATTGAAGGAGGCAATTTCAGCTGCCCCTTCACTAACCAACCTTTGCACACCATTGATTCCATTCCTAATCACAACATAAGGAAGATGATCCAAGCTTGGTGCGTCGAGAATAGGACTCATGGGATCGAGAGGATTCCGACGCCGAGGGTTCCCGCCAGCCCCGTGCAGGTTCGGGAGATTCTTGACAAGATCGCTGCTGCGGTCGGGCGGGAGGATTGTGAGGGATGCAAGTCCTTGGTTGAGAAGATCAAGATATTGGTACAAGAGAGTGAAAGGAACAAGAAATGCATTATTGCCAATGGAACAGGAAATGTATTATCCTCCGCATTCGAAGCATTTTCGAAACCCGAACGATTCGAGAAAAGGGTCGAGATTTTAGAAGAAATCTTATCGACGATGGCAATGATCTTCCCTCTACAAGAAGAAGCCATTAAACATCTAAAATCAGAAGCTTCAATGCAAGCATTGGTGTGGTTTGTAAGAGGAGAAGACAAATCAAGAAGAAGAAGCTCAATTTTGGTATTGAAAGAGATAATTTCATCATACCCTGAAAAAGTAAGCGAATTAGGAGAAATCCAAGGAGTATTAGAGGGTCTTATGAAGCTCATAAAAGAACCCATTTGTTCATCTTCAAAAAAGGCCTCACTTTTCATAACATATCATGCCATAGCCTCTTCTTCCAATCAAGAGGGCTTCACAAACGCATTTCTTCAAATGGGGTTGGTCCCTTTGCTACTCGAAACCCTCGTAGACGCCGAGAGAAGCGTATGCGAGAGGGCATTGGGAGTGTTCGATGGCATTTGCGAGACCAAACAAGGAAGAGAAGAGGCGTACGCGCATGCTTTGACGATGCCCGTGATTGTGAAAAAGATACTTAGAGTGTCGGATTTGGCAACCGAGCTATCGGTGTCGATCGTTTGGAAGCTCGTAAAGCACGAGAGTAGAGAAGAGGAAGATGAAGATGAAGATGGAGGAGTTAAAGTAGAAGCTCTTCAAGTTGGAGCATTTCAAAAGCTATTGTTGTTGTTGCAAGTTGGTTGTAGTGAATGGACAAAGGAGAAAGCAACTGAGTTGTTGAAACTCTTGAATAATCTTCATAGGGAAAGGTTAGAATGTATTGATTCTTTGGATTTTAAGGACCTCAAAAGAACCTTTTGA

Protein sequence

MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSLDFKDLKRTF
Homology
BLAST of Carg00144 vs. NCBI nr
Match: KAG6600854.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 854.4 bits (2206), Expect = 4.4e-244
Identity = 436/436 (100.00%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60
           MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP
Sbjct: 15  MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 74

Query: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120
           FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG
Sbjct: 75  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 134

Query: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180
           REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI
Sbjct: 135 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 194

Query: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240
           LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL
Sbjct: 195 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 254

Query: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300
           GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL
Sbjct: 255 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 314

Query: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360
           VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL
Sbjct: 315 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 374

Query: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420
           VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER
Sbjct: 375 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 434

Query: 421 LECIDSLDFKDLKRTF 437
           LECIDSLDFKDLKRTF
Sbjct: 435 LECIDSLDFKDLKRTF 450

BLAST of Carg00144 vs. NCBI nr
Match: KAG7031489.1 (U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854.4 bits (2206), Expect = 4.4e-244
Identity = 436/436 (100.00%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60
           MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP
Sbjct: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60

Query: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120
           FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG
Sbjct: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120

Query: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180
           REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI
Sbjct: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180

Query: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240
           LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL
Sbjct: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240

Query: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300
           GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL
Sbjct: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300

Query: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360
           VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL
Sbjct: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360

Query: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420
           VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER
Sbjct: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420

Query: 421 LECIDSLDFKDLKRTF 437
           LECIDSLDFKDLKRTF
Sbjct: 421 LECIDSLDFKDLKRTF 436

BLAST of Carg00144 vs. NCBI nr
Match: XP_022942676.1 (U-box domain-containing protein 21-like [Cucurbita moschata])

HSP 1 Score: 832.4 bits (2149), Expect = 1.8e-237
Identity = 430/436 (98.62%), Postives = 430/436 (98.62%), Query Frame = 0

Query: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60
           MAR GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP
Sbjct: 15  MAR-GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 74

Query: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120
           FTNQPLHTIDSIPNHNIRKMIQAWCVENR HGIERIPTPRVPASPVQVREILDKIAAAV 
Sbjct: 75  FTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVR 134

Query: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180
           REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI
Sbjct: 135 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 194

Query: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240
           LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL
Sbjct: 195 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 254

Query: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300
           GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT AFLQMGLVPLLLETL
Sbjct: 255 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL 314

Query: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360
           VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDL TELSVSIVWKL
Sbjct: 315 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKL 374

Query: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420
           VKHESREEE EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER
Sbjct: 375 VKHESREEE-EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 434

Query: 421 LECIDSLDFKDLKRTF 437
           LECIDSLDFKDLKRTF
Sbjct: 435 LECIDSLDFKDLKRTF 448

BLAST of Carg00144 vs. NCBI nr
Match: XP_023514876.1 (U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 827.4 bits (2136), Expect = 5.8e-236
Identity = 427/436 (97.94%), Postives = 429/436 (98.39%), Query Frame = 0

Query: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60
           MAR GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP
Sbjct: 14  MAR-GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 73

Query: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120
           FTNQPLHTIDSIPNHNIRKMIQAWCVENR HGIERIPTP+VPASPVQVREILDKIAAAVG
Sbjct: 74  FTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPKVPASPVQVREILDKIAAAVG 133

Query: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180
           REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI
Sbjct: 134 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 193

Query: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240
           L TMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL
Sbjct: 194 LWTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 253

Query: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300
           GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT AFLQMGLVPLLLETL
Sbjct: 254 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL 313

Query: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360
           VDAERSVCERALGVFDGICETK+GREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL
Sbjct: 314 VDAERSVCERALGVFDGICETKRGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 373

Query: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420
           VKHESR  E EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER
Sbjct: 374 VKHESR--EGEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 433

Query: 421 LECIDSLDFKDLKRTF 437
           LECIDSLDFKDLKRTF
Sbjct: 434 LECIDSLDFKDLKRTF 446

BLAST of Carg00144 vs. NCBI nr
Match: XP_022996865.1 (U-box domain-containing protein 21-like [Cucurbita maxima])

HSP 1 Score: 819.7 bits (2116), Expect = 1.2e-233
Identity = 419/430 (97.44%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 7   GGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPL 66
           GGGGGSM ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPL
Sbjct: 18  GGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPL 77

Query: 67  HTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEG 126
           HTIDSIPNHNIRKMIQAWCVENR HGIERIPTPRVPASPVQVREILDKIAAA GREDCE 
Sbjct: 78  HTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAAGREDCEE 137

Query: 127 CKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAM 186
           CKS+VEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAM
Sbjct: 138 CKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAM 197

Query: 187 IFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGV 246
           IFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGV
Sbjct: 198 IFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGV 257

Query: 247 LEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERS 306
           LEG+MKLIKEPICSSSKKASLFITYHAIASSSNQEGFT AFLQMGLV LLLETL+DAERS
Sbjct: 258 LEGVMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVSLLLETLIDAERS 317

Query: 307 VCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESR 366
           VCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESR
Sbjct: 318 VCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESR 377

Query: 367 EEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDS 426
           E+EDE EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDS
Sbjct: 378 EDEDE-EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDS 437

Query: 427 LDFKDLKRTF 437
           LDFKDLKRTF
Sbjct: 438 LDFKDLKRTF 446

BLAST of Carg00144 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.1e-99
Identity = 201/424 (47.41%), Postives = 272/424 (64.15%), Query Frame = 0

Query: 14  AELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIP 73
           +E+TIP  F CPIS+DLMKDPV +STGITYDR +IETWI  GN +CP TN  L T D IP
Sbjct: 27  SEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIP 86

Query: 74  NHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEK 133
           NH IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+++A  R D E C  ++EK
Sbjct: 87  NHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEK 146

Query: 134 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 193
           IK L  ESE+N+KC+  N  G VL   F+ FS     EK   +L EILS +  +FP+  E
Sbjct: 147 IKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLE 206

Query: 194 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKL 253
            I  L S  S + +   ++  D S R+++  ++KEI+S    +V       GV E L+KL
Sbjct: 207 GISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKL 266

Query: 254 IKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERSVCERALG 313
           I++ + SSS K+SL   Y  +          + FL++GLV + +E +VDAE SVCE+AL 
Sbjct: 267 IRDSVSSSSTKSSLIAIYQMVLQKPE---IASEFLEIGLVSITVEMIVDAENSVCEKALA 326

Query: 314 VFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDE 373
           V D ICET+ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    +   
Sbjct: 327 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVE--- 386

Query: 374 DGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSL-DFKDL 433
                 +A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK +
Sbjct: 387 ------DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYI 435

Query: 434 KRTF 437
           K+ F
Sbjct: 447 KKPF 435

BLAST of Carg00144 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 5.3e-83
Identity = 182/426 (42.72%), Postives = 259/426 (60.80%), Query Frame = 0

Query: 15  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPN 74
           E+TIPS F CPIS +LMKDPV +++GITYDR NIE W E G  +CP TN  L +++ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 75  HNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEKI 134
           H IR+MIQ WC  +   GIERIPTPRVP +  QV EI ++++AA  R D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 135 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 194
             L +ESERN+KC+  NG G VL   F+AFS+         +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL---LLEETVSVLTWMLPIGLEG 209

Query: 195 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLI 254
              L + +S   LV  +R  D    +++  ++KE++      V  L +I GV E  MK I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 255 KEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERSVCERALGV 314
                 S+   SL   +H I   +NQE   + FL++ LV + +E LVD+E SVCE+AL V
Sbjct: 270 NR---DSTCVNSLISIHHMIL--TNQE-TVSRFLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 315 FDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDED 374
            + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K           D
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK---------SGD 389

Query: 375 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER----LECIDSLDFK 434
           G    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +           S++FK
Sbjct: 390 GSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFK 431

Query: 435 DLKRTF 437
            +K+ F
Sbjct: 450 HVKKPF 431

BLAST of Carg00144 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.3e-53
Identity = 139/420 (33.10%), Postives = 219/420 (52.14%), Query Frame = 0

Query: 16  LTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIE-GGNFSCPFTNQPLHTIDSIPN 75
           + IP HF CPISL+LM+DPVT+ TG TYDRA+IE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 76  HNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGRE-DCEGCKSLVEK 135
           H +R++IQ WCV NR++G+ERIPTP+ PA P  VR +L + +A  G         + + +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 136 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 195
           ++   ++S++N+  I A+    +L      FS+        E++ E L+ + M+   +  
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESLALLVMLPITEPN 191

Query: 196 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEI---QGVLEGL 255
               + S+      +  +  +     R +   L EI+S+  +     G I   + V EG+
Sbjct: 192 QFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGV 251

Query: 256 MKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL-VDAERSVCE 315
           + L++ PI   S + +L I    + +  + +   +  +  G   +L++ L  D +R   E
Sbjct: 252 LDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTE 311

Query: 316 RALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEE 375
           RAL   + +C T +G      HALT+P++VK ILRVSD ATE +   +  L   E R  E
Sbjct: 312 RALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERWRE 371

Query: 376 DEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSLDF 430
                     EA   G   +LLL++Q  C+E  K+KA +LLKLL +   +     +S DF
Sbjct: 372 ----------EAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDF 412

BLAST of Carg00144 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 6.3e-52
Identity = 136/405 (33.58%), Postives = 211/405 (52.10%), Query Frame = 0

Query: 16  LTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPNH 75
           + IP HF CPISLDLM DPVT+STG TYDR +I++WI  GN +CP T   L     IPNH
Sbjct: 12  IQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTLIPNH 71

Query: 76  NIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGRE-DCEGCKSLVEKI 135
            +R++IQ WCV NR++G+ERIPTP+ PA P+ VR +L + +A  G         + + ++
Sbjct: 72  TLRRLIQEWCVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTHVSVRSRAAAIRRL 131

Query: 136 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 195
           + L ++SE+N+  I  +    +L      F+  E      E++ E L+ + ++  + E  
Sbjct: 132 RGLARDSEKNRVLIAGHNAREILVRIL--FADIETTSLSSELVSESLALLVLLH-MTETE 191

Query: 196 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQG---VLEGLM 255
            + + S+ S    +  +  +     R +   L E++ +  + +     I G   + EG++
Sbjct: 192 CEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIFEGVL 251

Query: 256 KLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL-VDAERSVCER 315
            L+K PI   S + +L I   AI +    +   +  +  G   +L++ L  D +R   ER
Sbjct: 252 DLLKNPI---SSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTER 311

Query: 316 ALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEED 375
            L   + +C   +G      HALT+P++VK ILRVSD ATE +   +  L   E R    
Sbjct: 312 GLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALCTAEER---- 371

Query: 376 EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNN 416
                  + EA   G   +LLLL+Q  C+E  K KA  LLKLL +
Sbjct: 372 ------CRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRD 400

BLAST of Carg00144 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.5e-48
Identity = 130/435 (29.89%), Postives = 226/435 (51.95%), Query Frame = 0

Query: 15  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWI-EGGNFSCPFTNQPLHTIDSIP 74
           E+ IPS FLCPISLD+MKDPV +STGITYDR +IE W+  G   SCP T Q +   D  P
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 75  NHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAA-VGREDCEGCKSLVE 134
           NH +R++IQ+WC  N ++GIERIPTP+ P    ++ +++ + +++ + +  C      ++
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLIKESSSSHLNQVKC------LK 123

Query: 135 KIKILVQESERNKKCI----IANGTGNVLSSAFEAFSKP-------------------ER 194
           +++ +V E+  NK+C+    +     N++S++ + ++ P                    R
Sbjct: 124 RLRQIVSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENR 183

Query: 195 FEKRVEILEEILSTMAMIFPLQEEAIKHLKSEASMQALVWFV-----RGEDKSRRRSSIL 254
           F+    +++E LS +  +    E A+K L +      LV  +     RG  +SR  +++L
Sbjct: 184 FDSSRSLMDEALSVLYHL-DTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAALL 243

Query: 255 VLKEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT 314
           + K +  + P ++  L   + +   +++++ + I   + ++++ I         N+    
Sbjct: 244 LKKLLEVADPMQIILLE--RELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAV 303

Query: 315 NAFLQMGLVPLLLETLVDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILR 374
                  ++ LL++    +ER   E A+ V D +C+  +GR E   H   + V+ KKILR
Sbjct: 304 EGGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILR 363

Query: 375 VSDLATELSVSIVWKLVKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKE 420
           VS + +E +V ++  + +  +           +  E LQ+G   KL L+LQV C   TKE
Sbjct: 364 VSQITSERAVRVLLSVGRFCATP--------SLLQEMLQLGVVAKLCLVLQVSCGNKTKE 421

BLAST of Carg00144 vs. ExPASy TrEMBL
Match: A0A6J1FPJ6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.7e-238
Identity = 430/436 (98.62%), Postives = 430/436 (98.62%), Query Frame = 0

Query: 1   MARGGGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 60
           MAR GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP
Sbjct: 15  MAR-GGGGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCP 74

Query: 61  FTNQPLHTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVG 120
           FTNQPLHTIDSIPNHNIRKMIQAWCVENR HGIERIPTPRVPASPVQVREILDKIAAAV 
Sbjct: 75  FTNQPLHTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAVR 134

Query: 121 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 180
           REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI
Sbjct: 135 REDCEGCKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEI 194

Query: 181 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 240
           LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL
Sbjct: 195 LSTMAMIFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSEL 254

Query: 241 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL 300
           GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFT AFLQMGLVPLLLETL
Sbjct: 255 GEIQGVLEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVPLLLETL 314

Query: 301 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKL 360
           VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDL TELSVSIVWKL
Sbjct: 315 VDAERSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLVTELSVSIVWKL 374

Query: 361 VKHESREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 420
           VKHESREEE EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER
Sbjct: 375 VKHESREEE-EDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER 434

Query: 421 LECIDSLDFKDLKRTF 437
           LECIDSLDFKDLKRTF
Sbjct: 435 LECIDSLDFKDLKRTF 448

BLAST of Carg00144 vs. ExPASy TrEMBL
Match: A0A6J1K7Y6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 PE=4 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 5.8e-234
Identity = 419/430 (97.44%), Postives = 423/430 (98.37%), Query Frame = 0

Query: 7   GGGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPL 66
           GGGGGSM ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPL
Sbjct: 18  GGGGGSMTELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPL 77

Query: 67  HTIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEG 126
           HTIDSIPNHNIRKMIQAWCVENR HGIERIPTPRVPASPVQVREILDKIAAA GREDCE 
Sbjct: 78  HTIDSIPNHNIRKMIQAWCVENRAHGIERIPTPRVPASPVQVREILDKIAAAAGREDCEE 137

Query: 127 CKSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAM 186
           CKS+VEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAM
Sbjct: 138 CKSMVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAM 197

Query: 187 IFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGV 246
           IFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGV
Sbjct: 198 IFPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGV 257

Query: 247 LEGLMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERS 306
           LEG+MKLIKEPICSSSKKASLFITYHAIASSSNQEGFT AFLQMGLV LLLETL+DAERS
Sbjct: 258 LEGVMKLIKEPICSSSKKASLFITYHAIASSSNQEGFTKAFLQMGLVSLLLETLIDAERS 317

Query: 307 VCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESR 366
           VCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESR
Sbjct: 318 VCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESR 377

Query: 367 EEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDS 426
           E+EDE EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDS
Sbjct: 378 EDEDE-EDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDS 437

Query: 427 LDFKDLKRTF 437
           LDFKDLKRTF
Sbjct: 438 LDFKDLKRTF 446

BLAST of Carg00144 vs. ExPASy TrEMBL
Match: A0A0A0KMI8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.7e-201
Identity = 363/432 (84.03%), Postives = 389/432 (90.05%), Query Frame = 0

Query: 8   GGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLH 67
           GG     ELTIP+HF CPISLDLMKDPVT+STGITYDRA+IETWIEGGNF+CPFTNQPL 
Sbjct: 17  GGLSHTIELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQPLQ 76

Query: 68  TIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGC 127
           TIDSIPNHNIRKMIQ WCVENR +GIERIPTPRVPASP QVREIL+K+AAA  R DC+GC
Sbjct: 77  TIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVREILEKMAAAARRGDCDGC 136

Query: 128 KSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMI 187
           KS+VEKIK L +ESERNKKCIIANGTGN LSS FEAFS PE FEKR+EILEEILS M + 
Sbjct: 137 KSMVEKIKKLGKESERNKKCIIANGTGNTLSSIFEAFSNPETFEKRIEILEEILSAMTVA 196

Query: 188 FPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVL 247
           FPLQEE+IKHLKSE S+Q+LVWF++G D S RR+S+LVLKEIISSYPEKV ELGEIQG L
Sbjct: 197 FPLQEESIKHLKSETSLQSLVWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGEIQGAL 256

Query: 248 EGLMKLIKEPICSSSKKASLFITYHAIA---SSSNQEGFTNAFLQMGLVPLLLETLVDAE 307
           EGL+KLIK+PICSSS+KASLFITYH IA   SSSN+E FT   LQMGLV LLLETLVDAE
Sbjct: 257 EGLIKLIKDPICSSSRKASLFITYHVIASTSSSSNRERFTKELLQMGLVSLLLETLVDAE 316

Query: 308 RSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHE 367
           RSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSV IVWKLVKHE
Sbjct: 317 RSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVCIVWKLVKHE 376

Query: 368 SREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECI 427
           S+EE    EDGG+KVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR+RLECI
Sbjct: 377 SKEE----EDGGIKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECI 436

Query: 428 DSLDFKDLKRTF 437
           DSLDFKDLKRTF
Sbjct: 437 DSLDFKDLKRTF 444

BLAST of Carg00144 vs. ExPASy TrEMBL
Match: A0A5D3BPA4 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold701G00820 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.7e-199
Identity = 360/432 (83.33%), Postives = 387/432 (89.58%), Query Frame = 0

Query: 8   GGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLH 67
           G  G   ELTIP+HF CPISLDLMKDPVT+STGITYDRA+IETWIEGGNF+CPFTNQPL 
Sbjct: 4   GSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQPLQ 63

Query: 68  TIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGC 127
           TIDSIPNHNIRKMIQ WCVENR +GIERIPTPRVPASP QVR+IL+K+ AA  R D EGC
Sbjct: 64  TIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDILEKMTAAARRGDFEGC 123

Query: 128 KSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMI 187
           KS+VEKIK L +ESERNKKCIIANGTGN  SS FEAFS PE FEKR+EILEEILS M M+
Sbjct: 124 KSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFEKRIEILEEILSAMTMV 183

Query: 188 FPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVL 247
           FPLQEE+IKHLKSE S+Q+L WF++G D S RR+S+LVLKEIISSYPEKV ELG IQG L
Sbjct: 184 FPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGVIQGAL 243

Query: 248 EGLMKLIKEPICSSSKKASLFITYHAIA---SSSNQEGFTNAFLQMGLVPLLLETLVDAE 307
           EGL+KLIK+PICSSSKKASLFITYH IA   SSSNQ  FT A LQMGLV LLLETLVDAE
Sbjct: 244 EGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALLQMGLVSLLLETLVDAE 303

Query: 308 RSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHE 367
           RSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVK+E
Sbjct: 304 RSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKNE 363

Query: 368 SREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECI 427
           S+EE+   EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR+RLECI
Sbjct: 364 SKEEK---EDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECI 423

Query: 428 DSLDFKDLKRTF 437
           DSLDFKDLKRTF
Sbjct: 424 DSLDFKDLKRTF 432

BLAST of Carg00144 vs. ExPASy TrEMBL
Match: A0A1S3CPH4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.7e-199
Identity = 360/432 (83.33%), Postives = 387/432 (89.58%), Query Frame = 0

Query: 8   GGGGSMAELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLH 67
           G  G   ELTIP+HF CPISLDLMKDPVT+STGITYDRA+IETWIEGGNF+CPFTNQPL 
Sbjct: 17  GSLGDPVELTIPTHFRCPISLDLMKDPVTLSTGITYDRASIETWIEGGNFTCPFTNQPLQ 76

Query: 68  TIDSIPNHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGC 127
           TIDSIPNHNIRKMIQ WCVENR +GIERIPTPRVPASP QVR+IL+K+ AA  R D EGC
Sbjct: 77  TIDSIPNHNIRKMIQDWCVENRAYGIERIPTPRVPASPAQVRDILEKMTAAARRGDFEGC 136

Query: 128 KSLVEKIKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMI 187
           KS+VEKIK L +ESERNKKCIIANGTGN  SS FEAFS PE FEKR+EILEEILS M M+
Sbjct: 137 KSMVEKIKNLGKESERNKKCIIANGTGNTFSSIFEAFSNPETFEKRIEILEEILSAMTMV 196

Query: 188 FPLQEEAIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVL 247
           FPLQEE+IKHLKSE S+Q+L WF++G D S RR+S+LVLKEIISSYPEKV ELG IQG L
Sbjct: 197 FPLQEESIKHLKSETSLQSLAWFLKGGDISGRRNSVLVLKEIISSYPEKVDELGVIQGAL 256

Query: 248 EGLMKLIKEPICSSSKKASLFITYHAIA---SSSNQEGFTNAFLQMGLVPLLLETLVDAE 307
           EGL+KLIK+PICSSSKKASLFITYH IA   SSSNQ  FT A LQMGLV LLLETLVDAE
Sbjct: 257 EGLIKLIKDPICSSSKKASLFITYHVIASTSSSSNQGRFTKALLQMGLVSLLLETLVDAE 316

Query: 308 RSVCERALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHE 367
           RSVCERALG FDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVK+E
Sbjct: 317 RSVCERALGAFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKNE 376

Query: 368 SREEEDEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECI 427
           S+EE+   EDGG++VEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHR+RLECI
Sbjct: 377 SKEEK---EDGGIEVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRDRLECI 436

Query: 428 DSLDFKDLKRTF 437
           DSLDFKDLKRTF
Sbjct: 437 DSLDFKDLKRTF 445

BLAST of Carg00144 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 2.2e-100
Identity = 201/424 (47.41%), Postives = 272/424 (64.15%), Query Frame = 0

Query: 14  AELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIP 73
           +E+TIP  F CPIS+DLMKDPV +STGITYDR +IETWI  GN +CP TN  L T D IP
Sbjct: 27  SEITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKTCPVTNTVLTTFDQIP 86

Query: 74  NHNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEK 133
           NH IRKMIQ WCVE  +  I+RIPTPRVP  P +V EI  K+++A  R D E C  ++EK
Sbjct: 87  NHTIRKMIQGWCVEKGSPLIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGVIIEK 146

Query: 134 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 193
           IK L  ESE+N+KC+  N  G VL   F+ FS     EK   +L EILS +  +FP+  E
Sbjct: 147 IKKLGDESEKNRKCVNENSVGWVLCDCFDKFSGD---EKLTFMLNEILSLLTWMFPIGLE 206

Query: 194 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKL 253
            I  L S  S + +   ++  D S R+++  ++KEI+S    +V       GV E L+KL
Sbjct: 207 GISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEALVKL 266

Query: 254 IKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERSVCERALG 313
           I++ + SSS K+SL   Y  +          + FL++GLV + +E +VDAE SVCE+AL 
Sbjct: 267 IRDSVSSSSTKSSLIAIYQMVLQKPE---IASEFLEIGLVSITVEMIVDAENSVCEKALA 326

Query: 314 VFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDE 373
           V D ICET+ GREE   +AL MP++VKKI +VS+LAT  S+S++ KL K  +    +   
Sbjct: 327 VLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWKTGNTVAVE--- 386

Query: 374 DGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSL-DFKDL 433
                 +A+++GAFQK+LL+LQVG  E TKEKATELLK++N   +   +C+DSL +FK +
Sbjct: 387 ------DAVRLGAFQKVLLVLQVGYGEETKEKATELLKMMNTQMKLMSDCVDSLKEFKYI 435

Query: 434 KRTF 437
           K+ F
Sbjct: 447 KKPF 435

BLAST of Carg00144 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 309.7 bits (792), Expect = 3.8e-84
Identity = 182/426 (42.72%), Postives = 259/426 (60.80%), Query Frame = 0

Query: 15  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPN 74
           E+TIPS F CPIS +LMKDPV +++GITYDR NIE W E G  +CP TN  L +++ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 75  HNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEKI 134
           H IR+MIQ WC  +   GIERIPTPRVP +  QV EI ++++AA  R D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 135 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 194
             L +ESERN+KC+  NG G VL   F+AFS+         +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL---LLEETVSVLTWMLPIGLEG 209

Query: 195 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLI 254
              L + +S   LV  +R  D    +++  ++KE++      V  L +I GV E  MK I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 255 KEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERSVCERALGV 314
                 S+   SL   +H I   +NQE   + FL++ LV + +E LVD+E SVCE+AL V
Sbjct: 270 NR---DSTCVNSLISIHHMIL--TNQE-TVSRFLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 315 FDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDED 374
            + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K           D
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK---------SGD 389

Query: 375 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER----LECIDSLDFK 434
           G    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +           S++FK
Sbjct: 390 GSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFK 431

Query: 435 DLKRTF 437
            +K+ F
Sbjct: 450 HVKKPF 431

BLAST of Carg00144 vs. TAIR 10
Match: AT1G66160.2 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 279.3 bits (713), Expect = 5.4e-75
Identity = 172/426 (40.38%), Postives = 246/426 (57.75%), Query Frame = 0

Query: 15  ELTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIEGGNFSCPFTNQPLHTIDSIPN 74
           E+TIPS F CPIS +LMKDP                W E G  +CP TN  L +++ IPN
Sbjct: 30  EITIPSQFQCPISYELMKDP----------------WFESGYQTCPVTNTVLTSLEQIPN 89

Query: 75  HNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEKI 134
           H IR+MIQ WC  +   GIERIPTPRVP +  QV EI ++++AA  R D   C  +V K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 135 KILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEEA 194
             L +ESERN+KC+  NG G VL   F+AFS+         +LEE +S +  + P+  E 
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASL---LLEETVSVLTWMLPIGLEG 209

Query: 195 IKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLI 254
              L + +S   LV  +R  D    +++  ++KE++      V  L +I GV E  MK I
Sbjct: 210 QSKLTTTSSFNRLVELLRNGD----QNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 255 KEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETLVDAERSVCERALGV 314
                 S+   SL   +H I   +NQE   + FL++ LV + +E LVD+E SVCE+AL V
Sbjct: 270 NR---DSTCVNSLISIHHMIL--TNQE-TVSRFLELDLVNITVEMLVDSENSVCEKALTV 329

Query: 315 FDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDED 374
            + ICETK+GRE+   + L +P++VKKIL++S+   +  VS++WK+ K           D
Sbjct: 330 LNVICETKEGREKVRRNKLVIPILVKKILKISE--KKDLVSVMWKVCK---------SGD 389

Query: 375 GGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRER----LECIDSLDFK 434
           G    EAL++GAF+KL+++LQVGC E TKEK TELLK++N + +           S++FK
Sbjct: 390 GSEVEEALRLGAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMKMNGFVDRSYSSSIEFK 415

Query: 435 DLKRTF 437
            +K+ F
Sbjct: 450 HVKKPF 415

BLAST of Carg00144 vs. TAIR 10
Match: AT3G02840.1 (ARM repeat superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.7e-66
Identity = 149/349 (42.69%), Postives = 227/349 (65.04%), Query Frame = 0

Query: 93  IERIPTPRVPASPVQVREILDKIAAAVGREDCEGCKSLVEKIKILVQESERNKKCIIANG 152
           IERI +PRV  +P    EI  ++  A  RE+   C  +V KIK L +  + NKKC++ NG
Sbjct: 40  IERIQSPRVLLTPRDAVEISRRLQNAAAREEYAECLEIVSKIKNLGRGGDTNKKCLVQNG 99

Query: 153 TGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPL-QEEAIKHLKSEASMQALVWFV 212
           +   LSS FE F+     +  + +LEEIL  ++   PL + E    + S AS+  LV F+
Sbjct: 100 SVLALSSCFERFAAAR--DGHMRLLEEILFVLSSWLPLNRSEGFNKMGSTASLNCLVRFL 159

Query: 213 RGEDKSRRRSSILVLKEIISSYPEKVSELGEIQGVLEGLMKLIKEPICSSSKKASLFITY 272
            G+D   R+++   ++E+I+     V  L +++G  EGL+K+I++ + +SS KASL + Y
Sbjct: 160 NGKDAKTRQNAAFCIREVIAVDKRYVYALTDVEGACEGLVKIIRDSVSTSSTKASLMVIY 219

Query: 273 HAIASSSNQEGFTNAFLQMGLVPLLLETLV-DAERSVCERALGVFDGICETKQGREEAYA 332
            AI+S+   +  T  F+++GLV L+ E +V +AE+SVCER+L V +  C+ +QG+E+   
Sbjct: 220 RAISSN---DKITEKFVKLGLVELITEMMVNNAEKSVCERSLVVLNATCDNEQGKEDVLR 279

Query: 333 HALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEEDEDEDGGVKVEALQVGAFQKL 392
           +AL +P++VKKILRVSDLAT+ SVSI+WKL K+    ++D + D  + VEALQVGAF+KL
Sbjct: 280 NALIVPLLVKKILRVSDLATQCSVSILWKLWKN----KKDGECDDRLLVEALQVGAFEKL 339

Query: 393 LLLLQVGCSEWTKEKATELLKLLNNLHR--ERLECID-SLDFKDLKRTF 437
           L+LLQVGC + TKEKA+ELL+ LN      E+  C+D S+  K++K++F
Sbjct: 340 LVLLQVGCEDKTKEKASELLRNLNRCRNEIEKTNCVDSSMHLKNVKKSF 379

BLAST of Carg00144 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-54
Identity = 139/420 (33.10%), Postives = 219/420 (52.14%), Query Frame = 0

Query: 16  LTIPSHFLCPISLDLMKDPVTMSTGITYDRANIETWIE-GGNFSCPFTNQPLHTIDSIPN 75
           + IP HF CPISL+LM+DPVT+ TG TYDRA+IE+W+  G N +CP T  PL     IPN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 76  HNIRKMIQAWCVENRTHGIERIPTPRVPASPVQVREILDKIAAAVGRE-DCEGCKSLVEK 135
           H +R++IQ WCV NR++G+ERIPTP+ PA P  VR +L + +A  G         + + +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 136 IKILVQESERNKKCIIANGTGNVLSSAFEAFSKPERFEKRVEILEEILSTMAMIFPLQEE 195
           ++   ++S++N+  I A+    +L      FS+        E++ E L+ + M+   +  
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKIL--FSE----TTSSELVSESLALLVMLPITEPN 191

Query: 196 AIKHLKSEASMQALVWFVRGEDKSRRRSSILVLKEIISSYPEKVSELGEI---QGVLEGL 255
               + S+      +  +  +     R +   L EI+S+  +     G I   + V EG+
Sbjct: 192 QFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGV 251

Query: 256 MKLIKEPICSSSKKASLFITYHAIASSSNQEGFTNAFLQMGLVPLLLETL-VDAERSVCE 315
           + L++ PI   S + +L I    + +  + +   +  +  G   +L++ L  D +R   E
Sbjct: 252 LDLLRNPI---SSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTE 311

Query: 316 RALGVFDGICETKQGREEAYAHALTMPVIVKKILRVSDLATELSVSIVWKLVKHESREEE 375
           RAL   + +C T +G      HALT+P++VK ILRVSD ATE +   +  L   E R  E
Sbjct: 312 RALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALCTAEERWRE 371

Query: 376 DEDEDGGVKVEALQVGAFQKLLLLLQVGCSEWTKEKATELLKLLNNLHRERLECIDSLDF 430
                     EA   G   +LLL++Q  C+E  K+KA +LLKLL +   +     +S DF
Sbjct: 372 ----------EAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDF 412

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6600854.14.4e-244100.00U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7031489.14.4e-244100.00U-box domain-containing protein 21, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022942676.11.8e-23798.62U-box domain-containing protein 21-like [Cucurbita moschata][more]
XP_023514876.15.8e-23697.94U-box domain-containing protein 21-like [Cucurbita pepo subsp. pepo][more]
XP_022996865.11.2e-23397.44U-box domain-containing protein 21-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q5PNY63.1e-9947.41U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Q9C8D15.3e-8342.72U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1... [more]
Q9LT793.3e-5333.10U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA46.3e-5233.58U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Q9SVC65.5e-4829.89E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FPJ68.7e-23898.62RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111447642... [more]
A0A6J1K7Y65.8e-23497.44RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111491979 P... [more]
A0A0A0KMI81.7e-20184.03RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G023870 PE... [more]
A0A5D3BPA44.7e-19983.33RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CPH44.7e-19983.33RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103503164 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G37490.12.2e-10047.41ARM repeat superfamily protein [more]
AT1G66160.13.8e-8442.72CYS, MET, PRO, and GLY protein 1 [more]
AT1G66160.25.4e-7540.38CYS, MET, PRO, and GLY protein 1 [more]
AT3G02840.12.7e-6642.69ARM repeat superfamily protein [more]
AT3G19380.12.4e-5433.10plant U-box 25 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 21..84
e-value: 2.8E-28
score: 110.0
IPR003613U box domainPFAMPF04564U-boxcoord: 18..89
e-value: 5.7E-20
score: 71.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 17..91
score: 35.632244
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 107..413
e-value: 5.5E-13
score: 50.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 11..95
e-value: 7.2E-28
score: 98.5
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 12..436
NoneNo IPR availablePANTHERPTHR22849:SF139ARM REPEAT SUPERFAMILY PROTEINcoord: 12..436
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 22..64
e-value: 1.5156E-22
score: 88.0594
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 11..94
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 122..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00144-RACarg00144-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity