Carg00099 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00099
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	gibberellin 2-beta-dioxygenase 8-like
Location	Carg_Chr04: 5289747 .. 5291814 (+)
RNA-Seq Expression	Carg00099
Synteny	Carg00099

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAACCCACAAGGAATATAGAGAACACTACCATGACGGACTCCAACCCACCACTCTTCCATCAATATGAGTTGCTTTTGCAATCCAAAGCTCAATTACAACTACTTAACAATGGCAATCAACAAATTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTACAGGTTTGGAAAGTAGCAATGAAAAGGAGAGGAGGGCTTGCAGTAGAGAAATTTTTGGAGCGTCGGCAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAGCAGAATGAGCAAAGAACAAACGAAGTTATTTGGGGTACCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATCATTCTTACAGATGGGGAACACCGACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTTCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAGTTCATTAACTTAAGGTATCAACTTTATCACCCTTCATCCAGCTACATACTTCTCTTACGTATTGTATTTTTTTCATTTAGTTCCAAGACTTGAAACGTTGTGTCGATATGATCTTTTAGACTTAAAAAAAGTTCAATTATATTCTTAAGTTTTAGACTAATTTCAGATTACGTTTTTGTTTGTTAATCTCACATTTACAGAAGGCTGGTATGGCATGCTTGTTAATATATCCAACCCTATGCGATACCATGCCAACTCAGCCAAAAGTCATCTAATGATCATGTCATATTCATATTTTAGTTCAAATTTGAGGAACATAAATGAAAATTTCAAAAGTTCTGTTTGTAATCTAACCAGATATCTTTTATATTATAATTTTTAATGCACTTTTCACCAACATCCCGCAATCTTGAAGAGTTTAACACGTCTAGACATTTTCACAAACAGCTAGACCGCAAAAAGTAATAGCACCTCGAAATCACAATCAACCGGCATTAAATGGCCACATTCTTCCACAATTGAACTAAATTACAAAAAAAAAAAAAAAAACAAGGAGAACCCACTACTCACTGACCTTATATGCTTGAACGCCAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGTTAGCAAGATCTTTAGCAGGAGTTTTAGTGGAGTGTTTGGGCCACCGAAAAGAAATATTTGACGATATCTGCGACGAAAGCACATGCTTCCTCCGATTGAATCATTACCCAATCTGTCCATTTCCCGGCGATGTTTCCGGCCTGGTGCCCCACACGGACAGCGATTTTCTTACCATACTTCATCAAGATCGCAGCGGCGGGCTGCAGCTGATGAAAGGATCGCAATGGGTGGCTGTAAAACCCAATCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTACAGGTACGCGCTGCAACTCTTTTACTTCGTTTTGATTATGACTAGGGTTAAATTTGTAAATTGGTCTACGTGGTTTGACGAGGTTAGATTCAATTTAATCTATTTTTGTTTTATTTTCTATTTTCAACCCTTAAACAAAATTTAAAACACCAAAGCATATATTCCTTTTTAATTTCAAAAGTTCTATTTTGTCCCTAATAAATTATTTAAATCATAAAATCAATTATTGTAATGTGACAATGTACCTAAAATTTTAAAAGTATTATAATAAGACCATACCAATTCGATTTAGTCAAGTCGGTTGGTCATCGGCTTCTGCAATTATATCATAAAAAATAAAAAATAGAAGTTGGTTTAAAATATATTTATCGATCGACAAATAATTCAACCCCTTTCAATTTCAGACTATAAGTCTTCTTACGCAAAATATCATGAACCGATAAGCTGATCTGCAGGCTTGGAGCAATGATACTTACAAGAGTGTGAAGCACAGGGTCGTGGCTAATTCAATGCGGGAAAGATATTCGATAGCCTACTTCCTCTGCCCTTCTTATCATTCTCCAATTGGGAGTTGCAGACAGCCTTCACCGTATAGACACTTCACTTTTGCTGAGTACAGAAAGCAAGTTAGAGATGATGTGGAAAAAATTGGGTACAAAGTAGGTCTTTCGAAATTTCGG

mRNA sequence

GAAACCCACAAGGAATATAGAGAACACTACCATGACGGACTCCAACCCACCACTCTTCCATCAATATGAGTTGCTTTTGCAATCCAAAGCTCAATTACAACTACTTAACAATGGCAATCAACAAATTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTACAGGTTTGGAAAGTAGCAATGAAAAGGAGAGGAGGGCTTGCAGTAGAGAAATTTTTGGAGCGTCGGCAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAGCAGAATGAGCAAAGAACAAACGAAGTTATTTGGGGTACCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATCATTCTTACAGATGGGGAACACCGACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTTCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAGTTCATTAACTTAAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGTTAGCAAGATCTTTAGCAGGAGTTTTAGTGGAGTGTTTGGGCCACCGAAAAGAAATATTTGACGATATCTGCGACGAAAGCACATGCTTCCTCCGATTGAATCATTACCCAATCTGTCCATTTCCCGGCGATGTTTCCGGCCTGGTGCCCCACACGGACAGCGATTTTCTTACCATACTTCATCAAGATCGCAGCGGCGGGCTGCAGCTGATGAAAGGATCGCAATGGGTGGCTGTAAAACCCAATCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTACAGGCTTGGAGCAATGATACTTACAAGAGTGTGAAGCACAGGGTCGTGGCTAATTCAATGCGGGAAAGATATTCGATAGCCTACTTCCTCTGCCCTTCTTATCATTCTCCAATTGGGAGTTGCAGACAGCCTTCACCGTATAGACACTTCACTTTTGCTGAGTACAGAAAGCAAGTTAGAGATGATGTGGAAAAAATTGGGTACAAAGTAGGTCTTTCGAAATTTCGG

Coding sequence (CDS)

ATGACGGACTCCAACCCACCACTCTTCCATCAATATGAGTTGCTTTTGCAATCCAAAGCTCAATTACAACTACTTAACAATGGCAATCAACAAATTAACTCTGTCATGGAGGAATGTCAGCTTCCTCTTATTGATCTTACAGGTTTGGAAAGTAGCAATGAAAAGGAGAGGAGGGCTTGCAGTAGAGAAATTTTTGGAGCGTCGGCAGAATGGGGATTCTTCCAAGTGATAAACCATGGGATACGCACAGAGCTGCTGAGCAGAATGAGCAAAGAACAAACGAAGTTATTTGGGGTACCCTTTGAGAAAAAATCTACAAGTGGGATTTTGGATCATTCTTACAGATGGGGAACACCGACTGCAACCCACCCAAACCAGTTCTCTTGGTCTGAAGCTTTTCACATTTCCCTCACAAAGGTCTCTGAAGCTGCCTGCTATGGCGAGTTCATTAACTTAAGGGAAGTGATGGAGGAACTGGCAACCGCCATGTCGAAGTTAGCAAGATCTTTAGCAGGAGTTTTAGTGGAGTGTTTGGGCCACCGAAAAGAAATATTTGACGATATCTGCGACGAAAGCACATGCTTCCTCCGATTGAATCATTACCCAATCTGTCCATTTCCCGGCGATGTTTCCGGCCTGGTGCCCCACACGGACAGCGATTTTCTTACCATACTTCATCAAGATCGCAGCGGCGGGCTGCAGCTGATGAAAGGATCGCAATGGGTGGCTGTAAAACCCAATCCAGAAGCCCTTGTTGTCAACATTGGAGATCTTCTACAGGCTTGGAGCAATGATACTTACAAGAGTGTGAAGCACAGGGTCGTGGCTAATTCAATGCGGGAAAGATATTCGATAGCCTACTTCCTCTGCCCTTCTTATCATTCTCCAATTGGGAGTTGCAGACAGCCTTCACCGTATAGACACTTCACTTTTGCTGAGTACAGAAAGCAAGTTAGAGATGATGTGGAAAAAATTGGGTACAAAGTAGGTCTTTCGAAATTTCGG

Protein sequence

MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPTATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR

Homology

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BLAST of Carg00099 vs. NCBI nr
Match: KAG7031445.1 (Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 694.5 bits (1791), Expect = 4.5e-196
Identity = 335/335 (100.00%), Postives = 335/335 (100.00%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR
Sbjct: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 335

[Close]

BLAST of Carg00099 vs. NCBI nr
Match: KAG6600809.1 (Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 687.2 bits (1772), Expect = 7.2e-194
Identity = 331/335 (98.81%), Postives = 332/335 (99.10%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 120

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 121 ATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDIC ESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 181 RKEIFDDICHESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           RQPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFR
Sbjct: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFR 335

[Close]

BLAST of Carg00099 vs. NCBI nr
Match: XP_022942053.1 (gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita moschata])

HSP 1 Score: 686.4 bits (1770), Expect = 1.2e-193
Identity = 330/335 (98.51%), Postives = 332/335 (99.10%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 41  MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 100

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 101 SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 160

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 161 ATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 220

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 221 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 280

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKH VVANSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 281 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHMVVANSMRERYSIAYFLCPSYHSPIGSC 340

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           +QPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFR
Sbjct: 341 KQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFR 375

[Close]

BLAST of Carg00099 vs. NCBI nr
Match: XP_022989785.1 (gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita maxima])

HSP 1 Score: 683.7 bits (1763), Expect = 7.9e-193
Identity = 329/335 (98.21%), Postives = 330/335 (98.51%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVM ECQLPLIDLTGLESSNEKERRAC
Sbjct: 41  MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMGECQLPLIDLTGLESSNEKERRAC 100

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGAS EWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 101 SREIFGASTEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 160

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 161 ATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 220

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 221 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 280

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAV PNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 281 WVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 340

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           RQPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFR
Sbjct: 341 RQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFR 375

[Close]

BLAST of Carg00099 vs. NCBI nr
Match: XP_023546537.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.2 bits (1759), Expect = 2.3e-192
Identity = 328/335 (97.91%), Postives = 330/335 (98.51%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 1   MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 120

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           A HPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 121 AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDICDESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 181 RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAVKPNPEALVVNIGDLLQ WSNDTYKSVKHRVV NSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 241 WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC 300

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR
Sbjct: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 335

[Close]

BLAST of Carg00099 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-96
Identity = 177/347 (51.01%), Postives = 230/347 (66.28%), Query Frame = 0


Query: 5   NPPLFHQYELLLQSKAQ---------LQLLNNGNQQINSVMEECQLPLIDLTGLESSNEK 64
           +PPL   Y  L   +           +Q++ +G   +     E  LP++DL  L      
Sbjct: 10  DPPLADSYRALALLRRDRDGGIAPPAVQMVGSGGAVL-----ERDLPMVDLERLTRGGAG 69

Query: 65  ERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYR 124
           ER+AC+  +  A++EWGFFQ+ NHG+  EL+  M +EQ +LF +PFE K  +G+L+ SYR
Sbjct: 70  ERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRLPFETKEKAGLLNGSYR 129

Query: 125 WGTPTATHPNQFSWSEAFHISLTKVSEAAC-YGEFINLREVMEELATAMSKLARSLAGVL 184
           WG PTAT     SWSEAFH+ L  +S A C +G+  +LR VM+E+A AMS++A ++A  L
Sbjct: 130 WGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVMQEVAEAMSRVANTVAAAL 189

Query: 185 VECLGHR-------KEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQ 244
            E L  R          F   CDE+TCFLRLN YP CPF  D  GLVPHTDSDFLT+L Q
Sbjct: 190 AEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAADTFGLVPHTDSDFLTVLCQ 249

Query: 245 DRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAY 304
           D+ GGL LMK S+WVAV+P P+ALVVNIGDL QAWSN+ YKSV+H+VVAN+  +R S+AY
Sbjct: 250 DQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKSVEHKVVANAKTDRLSVAY 309

Query: 305 FLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 335
           FLCPSY S +G+C +PSPYR FTF EYRK+V++DV   G K+GL  F
Sbjct: 310 FLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKIGLPNF 351

[Close]

BLAST of Carg00099 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 332.0 bits (850), Expect = 7.7e-90
Identity = 159/304 (52.30%), Postives = 211/304 (69.41%), Query Frame = 0


Query: 32  INSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSK 91
           + +V+EE +LP+ID++ L    E+ER  C   I  AS EWGFFQVINHGI  ++L +M +
Sbjct: 33  VTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQ 92

Query: 92  EQTKLFGVPFEKKSTSGILD-HSYRWGTPTATHPNQFSWSEAFHISLTKVSEAACYGEFI 151
           EQ ++F  PF+KKS S      SYRWGTP+AT   Q SWSEAFH+ +T +S+     +F 
Sbjct: 93  EQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFT 152

Query: 152 NLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDV 211
            L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP P +V
Sbjct: 153 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEV 212

Query: 212 SGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSV 271
            GL+PHTDSDFLTIL+QD+ GGLQL+K ++W+AVKPNP+AL++NIGDL QAWSN  YKSV
Sbjct: 213 YGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSV 272

Query: 272 KHRVVANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVG 331
           +HRV+ N   ER+S AYF+CPSY + I        YR+F+F E+R+QV++DV+K G+KVG
Sbjct: 273 EHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVG 332

Query: 332 LSKF 335
           L +F
Sbjct: 333 LPRF 333

[Close]

BLAST of Carg00099 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-78
Identity = 161/346 (46.53%), Postives = 214/346 (61.85%), Query Frame = 0


Query: 2   TDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSV-MEECQLPLIDLTGLESSNEKERRAC 61
           ++SNPPL   Y+ L   + +L +       + ++ ++EC+LP+IDL GL  + +     C
Sbjct: 8   SNSNPPLMSTYKHLFVEQHRLDM------DMGAIDVDECELPVIDLAGLMEAEQ----VC 67

Query: 62  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTS---GILDHSYRWG 121
             ++  A++EWGFFQV NHG+   LL  +   Q  +F  PF++K T    G    SYRWG
Sbjct: 68  RADMVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTERLLGFSPESYRWG 127

Query: 122 TPTATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVEC 181
           TPTA    Q SWSEA+HI +T    +      I  R V+EE++ AM +LA+ LA +L+  
Sbjct: 128 TPTAKCLEQLSWSEAYHIPMTTPRPSTS----IRARAVIEEVSRAMYELAQKLAEILMRG 187

Query: 182 LGHRKEIFDDICD-ESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILH--QDRSGGLQ 241
           L    E    +   E TCFLRLN YP C       GL PHTDSD LTI+H  QD  GGLQ
Sbjct: 188 LPGAGEGETMVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQ 247

Query: 242 LMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYH 301
           L+KG +WVAVKP+P  L+VN+GDLLQAWSND YKSV+HRV+AN+  ER+S+A+FLCPSYH
Sbjct: 248 LLKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYH 307

Query: 302 SPIGSCR-----QPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           + I           + YR FTF EYRKQ+ +DV   G K+GL +FR
Sbjct: 308 TLIIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRFR 339

[Close]

BLAST of Carg00099 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.7e-69
Identity = 133/298 (44.63%), Postives = 192/298 (64.43%), Query Frame = 0


Query: 40  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGV 99
           +LP+IDL+ L S  E +R+ C +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 100 PFE---KKSTSGILDHSYRWGTPTATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVM 159
           PF    ++  S +  +SYRWG P+AT P Q+S SEAFHI L++VS  +   +  NLR ++
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIV 158

Query: 160 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPH 219
           E     ++++A+ +  +L + +    E F++I +    FLRLN Y    F  +V GLVPH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 220 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVA 279
           TD+ FLTIL QD+ GGL+L    QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 280 NSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 335
            +  ER SIA+F+CP   + I     P  YR F+F EY++Q   DV++ G KVGLS+F
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]

BLAST of Carg00099 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 6.2e-39
Identity = 102/293 (34.81%), Postives = 148/293 (50.51%), Query Frame = 0


Query: 41  LPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVP 100
           +P+IDL GL S            I  A   WGFFQV+NHG++ EL+    +   + F +P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 101 FEKKSTSGILDHSYR-WGTPTATHPN-QFSWSEAF--HISLTKVSEAACYGEF-INLREV 160
              K T      +Y  +G+           WS+ +  H+    + +   +  F   +REV
Sbjct: 122 VNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREV 181

Query: 161 MEELATAMSKLARSLAGVLVECLGHRKEIFDDIC--DESTCFLRLNHYPICPFPGDVSGL 220
           ++E    + KL+  +  VL   LG +++ F +    +     LR+N+YP CP P    GL
Sbjct: 182 IDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGL 241

Query: 221 VPHTDSDFLTI-LHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKH 280
            PH+D   +TI L  D+  GLQ+ K   W+ VKP+P A +VNIGD +Q  SN TYKSV+H
Sbjct: 242 SPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEH 301

Query: 281 RVVANSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAEYRKQVR 320
           RV+ NS +ER S+A+F  P    PI       S   P  Y   TF +YR  +R
Sbjct: 302 RVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIR 354

[Close]

BLAST of Carg00099 vs. ExPASy TrEMBL
Match: A0A6J1FTS8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447241 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 5.9e-194
Identity = 330/335 (98.51%), Postives = 332/335 (99.10%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 41  MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 100

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 101 SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 160

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 161 ATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 220

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 221 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 280

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKH VVANSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 281 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHMVVANSMRERYSIAYFLCPSYHSPIGSC 340

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           +QPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFR
Sbjct: 341 KQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFR 375

[Close]

BLAST of Carg00099 vs. ExPASy TrEMBL
Match: A0A6J1JNB9 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111486865 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 3.8e-193
Identity = 329/335 (98.21%), Postives = 330/335 (98.51%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVM ECQLPLIDLTGLESSNEKERRAC
Sbjct: 41  MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMGECQLPLIDLTGLESSNEKERRAC 100

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIFGAS EWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 101 SREIFGASTEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 160

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 161 ATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 220

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 221 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 280

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAV PNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 281 WVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 340

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           RQPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFR
Sbjct: 341 RQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFR 375

[Close]

BLAST of Carg00099 vs. ExPASy TrEMBL
Match: A0A0A0KIW0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 6.2e-167
Identity = 285/335 (85.07%), Postives = 303/335 (90.45%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           M DSNPPLF QYE LLQS A LQ  NNGN   NSVMEECQLPLIDL GL+SS+EKER AC
Sbjct: 9   MMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEKERVAC 68

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
            REIF AS EWGFFQVINHGI TELL+RM+KEQ KLFGVPFEKK TSGILD+SYRWGTPT
Sbjct: 69  RREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYRWGTPT 128

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           ATHPNQFSWSEAFHI LTKVSEAACYG+FI LREVMEE+A+AMSK+AR LAGVLVE +G 
Sbjct: 129 ATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLVESMGQ 188

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKE+ +DICDESTCFLRLNHYPICPF G+VSGLVPHTDSDFLTILHQD  GGLQ+MKGSQ
Sbjct: 189 RKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQVMKGSQ 248

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           W+AVKPNP+ALVVNIGDLLQAWSNDTYKS KHRVVAN MRERYSIAYFLCPSYHS IGSC
Sbjct: 249 WLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPSYHSQIGSC 308

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           R+PSPYRHFTFAEYRKQVRDDVEK GYK+GLS FR
Sbjct: 309 REPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFR 343

[Close]

BLAST of Carg00099 vs. ExPASy TrEMBL
Match: A0A6J1FVR3 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447734 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 4.0e-166
Identity = 287/335 (85.67%), Postives = 304/335 (90.75%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60
           M +SNPPLF QYELLLQS A LQ L+NG Q  +SV+EECQLPL+DL GLES  EKER AC
Sbjct: 8   MMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKERMAC 67

Query: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120
           SREIF ASAEWGFFQVINHGIR+ELLSRMSKEQ KLF VPFEKKSTSGILD+SYRWG  T
Sbjct: 68  SREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAAT 127

Query: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180
           AT PNQFSWSEAFHI LTKVSEA CYG+FI+LREVMEELATAMSKLARSLAGVLVE LGH
Sbjct: 128 ATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVESLGH 187

Query: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240
           RKE+F+D+CDESTCFLRLNHYPICPFPG+V+GLVPHTDSD+LTILHQD  GGLQLMKGSQ
Sbjct: 188 RKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQ 247

Query: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300
           WVAVKP PEALVVNIGDLLQAWSNDTYKSVKHRVV NS+RERYS AYFLCPS  S IGSC
Sbjct: 248 WVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSGIGSC 307

Query: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           RQPSPYRHFTF EYRKQVRDDVEK GYKVGLS+FR
Sbjct: 308 RQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFR 342

[Close]

BLAST of Carg00099 vs. ExPASy TrEMBL
Match: A0A6J1JB12 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483364 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.9e-164
Identity = 285/333 (85.59%), Postives = 299/333 (89.79%), Query Frame = 0


Query: 3   DSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRACSR 62
           +SNPPLFHQY LLLQS A LQ L +G Q  +SV+EECQLPL+DL GLES  EKER ACS 
Sbjct: 10  NSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKERMACSS 69

Query: 63  EIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPTAT 122
           EIF ASAEWGFFQVINHGIR+ELLSRMSKEQ KLF VPFEKKSTSGILD+SYRWG  TAT
Sbjct: 70  EIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRWGAATAT 129

Query: 123 HPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGHRK 182
            PNQFSWSEAFHI LTKVSEA CYG FI+LREVMEELATAMSKLARSLAGVLVE LGHRK
Sbjct: 130 QPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVESLGHRK 189

Query: 183 EIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWV 242
           E+F+D+CDESTCFLRLNHYPICPFPG V+GLVPHTDSD+LTILHQD  GGLQLMKGSQWV
Sbjct: 190 EVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLMKGSQWV 249

Query: 243 AVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSCRQ 302
           AVKP PEALVVNIGDLLQAWSNDTYKSVKHRVVANS RERYS AYFLCPS HS IGSCRQ
Sbjct: 250 AVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSGIGSCRQ 309

Query: 303 PSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 336
           PSPYRHFTF EYRKQVRDDVEK GYK GLS+FR
Sbjct: 310 PSPYRHFTFGEYRKQVRDDVEKTGYKEGLSRFR 342

[Close]

BLAST of Carg00099 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 332.0 bits (850), Expect = 5.4e-91
Identity = 159/304 (52.30%), Postives = 211/304 (69.41%), Query Frame = 0


Query: 32  INSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSK 91
           + +V+EE +LP+ID++ L    E+ER  C   I  AS EWGFFQVINHGI  ++L +M +
Sbjct: 33  VTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQ 92

Query: 92  EQTKLFGVPFEKKSTSGILD-HSYRWGTPTATHPNQFSWSEAFHISLTKVSEAACYGEFI 151
           EQ ++F  PF+KKS S      SYRWGTP+AT   Q SWSEAFH+ +T +S+     +F 
Sbjct: 93  EQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFT 152

Query: 152 NLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDV 211
            L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP P +V
Sbjct: 153 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEV 212

Query: 212 SGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSV 271
            GL+PHTDSDFLTIL+QD+ GGLQL+K ++W+AVKPNP+AL++NIGDL QAWSN  YKSV
Sbjct: 213 YGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSV 272

Query: 272 KHRVVANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVG 331
           +HRV+ N   ER+S AYF+CPSY + I        YR+F+F E+R+QV++DV+K G+KVG
Sbjct: 273 EHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVG 332

Query: 332 LSKF 335
           L +F
Sbjct: 333 LPRF 333

[Close]

BLAST of Carg00099 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 262.7 bits (670), Expect = 4.1e-70
Identity = 133/298 (44.63%), Postives = 192/298 (64.43%), Query Frame = 0


Query: 40  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGV 99
           +LP+IDL+ L S  E +R+ C +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 100 PFE---KKSTSGILDHSYRWGTPTATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVM 159
           PF    ++  S +  +SYRWG P+AT P Q+S SEAFHI L++VS  +   +  NLR ++
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIV 158

Query: 160 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPH 219
           E     ++++A+ +  +L + +    E F++I +    FLRLN Y    F  +V GLVPH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 220 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVA 279
           TD+ FLTIL QD+ GGL+L    QW++VKP  EAL VNIGD+ QA SN  Y+SV+HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 280 NSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 335
            +  ER SIA+F+CP   + I     P  YR F+F EY++Q   DV++ G KVGLS+F
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

[Close]

BLAST of Carg00099 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 4.0e-41
Identity = 117/371 (31.54%), Postives = 183/371 (49.33%), Query Frame = 0


Query: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVM---EECQLPLIDLTGLESSNEKER 60
           +++S PP F             ++ N+ N     V+   ++  +P+IDL  L+   E  R
Sbjct: 3   ISESYPPAFR------------RVTNDENPPATPVIVQGKDINIPVIDLERLD--KEILR 62

Query: 61  RACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKS---TSGILDHSY 120
            AC         EWG F++ NHG+   L SR+ +    L  +PFEKK     +     SY
Sbjct: 63  EACK--------EWGIFRLENHGVPLALTSRLQEISESLLSLPFEKKRELFAAVKSPLSY 122

Query: 121 RWGTPT----------ATHPNQFSWSEAFHI------SLTKVSEAACYGE-------FIN 180
            WGTP               +  +  E F++      SL+K+  + C  +         +
Sbjct: 123 FWGTPALNRSGDALKRGAQASNLTMLEGFNVPLSSLSSLSKLPTSTCCDDDAQEEPKLES 182

Query: 181 LREVMEELATAMSKLARSLAGVLVECL-----GHRKEIFDDICDESTCFLRLNHYPICPF 240
            R +MEE    ++++A SL   + + L     G+R+    +   EST  +R+  YP    
Sbjct: 183 FRVLMEEYGKHITRIAVSLFEAIAQTLNLELSGNRR---SEYLSESTGLIRVYRYPQSSE 242

Query: 241 PG--DVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSN 300
               +  G+  HTDS  ++IL +D SGGL++MKG +W  VKP    L+VN+GD++QA S+
Sbjct: 243 EAAREALGMEVHTDSSVISILREDESGGLEIMKGEEWFCVKPVANTLIVNLGDMMQAISD 302

Query: 301 DTYKSVKHRV-VANSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVE 335
           D YKSV HRV   N   ER+S+ YF+ P     I S    S Y+ FT++++  QV+ DV+
Sbjct: 303 DEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKS----SNYKLFTYSDFEAQVQADVQ 344

[Close]

BLAST of Carg00099 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 4.4e-40
Identity = 102/293 (34.81%), Postives = 148/293 (50.51%), Query Frame = 0


Query: 41  LPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVP 100
           +P+IDL GL S            I  A   WGFFQV+NHG++ EL+    +   + F +P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 101 FEKKSTSGILDHSYR-WGTPTATHPN-QFSWSEAF--HISLTKVSEAACYGEF-INLREV 160
              K T      +Y  +G+           WS+ +  H+    + +   +  F   +REV
Sbjct: 122 VNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREV 181

Query: 161 MEELATAMSKLARSLAGVLVECLGHRKEIFDDIC--DESTCFLRLNHYPICPFPGDVSGL 220
           ++E    + KL+  +  VL   LG +++ F +    +     LR+N+YP CP P    GL
Sbjct: 182 IDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGL 241

Query: 221 VPHTDSDFLTI-LHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKH 280
            PH+D   +TI L  D+  GLQ+ K   W+ VKP+P A +VNIGD +Q  SN TYKSV+H
Sbjct: 242 SPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEH 301

Query: 281 RVVANSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAEYRKQVR 320
           RV+ NS +ER S+A+F  P    PI       S   P  Y   TF +YR  +R
Sbjct: 302 RVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIR 354

[Close]

BLAST of Carg00099 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 5.7e-40
Identity = 107/312 (34.29%), Postives = 158/312 (50.64%), Query Frame = 0


Query: 40  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGV 99
           ++P IDL+ LE ++  ++ A ++EI  A   WGFFQVINHG+ + L  R+ K   + F +
Sbjct: 31  EIPTIDLSSLEDTHH-DKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 100 PFEKKSTSGILDHSYRWGTPTATH-PNQFSWSEAFHISL--TKVSEAACYGEFINL---- 159
             E+K      D     G     H  N   W E F   L  + +  A+   E   L    
Sbjct: 91  TTEEKRKVK-RDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLT 150

Query: 160 ----------REVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYP 219
                     REV +E A  + KLA  L  ++   LG   +      +E T FLR NHYP
Sbjct: 151 NQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYP 210

Query: 220 ICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGS--QWVAVKPNPEALVVNIGDLLQ 279
            CP P    G+  H D   LT+L QD  GGLQ+ + S  QW+ VKP  +AL++N+G+ +Q
Sbjct: 211 PCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQ 270

Query: 280 AWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAE- 326
            W+ND Y S +HRVV N+ +ER+SI +F  PS+ + I       S   P  Y+ + + + 
Sbjct: 271 VWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 330

The following BLAST results are available for this feature:

BLAST of Carg00099 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7031445.1	4.5e-196	100.00	Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyros...	[more]
KAG6600809.1	7.2e-194	98.81	Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022942053.1	1.2e-193	98.51	gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita moschata]	[more]
XP_022989785.1	7.9e-193	98.21	gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita maxima]	[more]
XP_023546537.1	2.3e-192	97.91	gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg00099 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7XP65	1.1e-96	51.01	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
O49561	7.7e-90	52.30	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q8LGZ9	1.8e-78	46.53	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q9C6I4	5.7e-69	44.63	Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 ...	[more]
Q9FFF6	6.2e-39	34.81	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]

BLAST of Carg00099 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTS8	5.9e-194	98.51	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
A0A6J1JNB9	3.8e-193	98.21	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148686...	[more]
A0A0A0KIW0	6.2e-167	85.07	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G...	[more]
A0A6J1FVR3	4.0e-166	85.67	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447...	[more]
A0A6J1JB12	4.9e-164	85.59	gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC11148336...	[more]

BLAST of Carg00099 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21200.1	5.4e-91	52.30	gibberellin 2-oxidase 8	[more]
AT1G50960.1	4.1e-70	44.63	gibberellin 2-oxidase 7	[more]
AT5G58660.1	4.0e-41	31.54	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G05600.1	4.4e-40	34.81	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G19000.1	5.7e-40	34.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 41..138 e-value: 3.5E-16 score: 60.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 195..291 e-value: 7.4E-27 score: 93.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 15..323 e-value: 2.3E-79 score: 269.1
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 30..327
None	No IPR available	PANTHER	PTHR47990:SF24	OS02G0630300 PROTEIN	coord: 30..327
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 33..318
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 190..291 score: 14.025037

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00099-RA	Carg00099-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding