Carg00063 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg00063
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	fatty acid desaturase 4, chloroplastic
Location	Carg_Chr04: 5104025 .. 5104981 (+)
RNA-Seq Expression	Carg00063
Synteny	Carg00063

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGATCTTAGTTCAAAACAAGTTCCTATTGAGCTCCAAAACACATGTTAGCCTGTGGAGCCCTGCGATTCAACGCAAATTTGTCTGCTGCATAGCCAATACCACAGTCAATGTTGCTGCTGCTACCACCGCCGCCGCCAAACCCAAGCACGGTGATGGCCAACTAGTCATTGAACGAGAGCCTTTATCTCCTACTACACCTGTATTGCCCAATTCCTCCACCTATTATTCTGTTCTTAATGATCCCAGTTTACAATCGACCTGGAGTCATCGTGTGTGGGTGACAAGTGGGTGCACCATAGTGCTAATCTCTTTAGCAAAATCAATAGCAGGTGCAGCCAGTACACATACCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCTGGGGTTTACCATTGGGGCATCGATAACTATGGCGACGCCTCAACTCCAATTTTTGGCACCCAAATAGAAGCATTCCAAGGCCATCATAAGCGGCCATGGACAATCACAAAGCGTCAATTTGCCAACAATCTGTATGCTTTGGCACGAGCTGTGACTTTCACTGTACTTCCTATAACCCTTGCATTTAATGACCCTATTCTTCATGGTTTCGTTGCCGTATGCTCAGGCTGTATAATGTTCAGCCAACAGTTCCATGCATGGGCTCACGACAAAAAGAGTCAACTACCACCACTCGTAGTAGCAATGCAAGATGCTGGCCTGCTAGTGTCGCGATTGCAGCATTCTGCACATCATCGTCCACCCTATAACAACAACTACTGCATAGTAAGCGGAATTTGGAATAAGCTTTTAGATGAAACAAGGGTTTTTGAGGCATTAGAGATGATCCTGTTCTTCAAGCTTGGGGTGAGACCAAGATCTTGGACCGAGCCTCATTCCGAATGGACAGAAGAGACTGAGATTATAGAACAAACTACAGCATATTAA

mRNA sequence

ATGTCGATCTTAGTTCAAAACAAGTTCCTATTGAGCTCCAAAACACATGTTAGCCTGTGGAGCCCTGCGATTCAACGCAAATTTGTCTGCTGCATAGCCAATACCACAGTCAATGTTGCTGCTGCTACCACCGCCGCCGCCAAACCCAAGCACGGTGATGGCCAACTAGTCATTGAACGAGAGCCTTTATCTCCTACTACACCTGTATTGCCCAATTCCTCCACCTATTATTCTGTTCTTAATGATCCCAGTTTACAATCGACCTGGAGTCATCGTGTGTGGGTGACAAGTGGGTGCACCATAGTGCTAATCTCTTTAGCAAAATCAATAGCAGGTGCAGCCAGTACACATACCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCTGGGGTTTACCATTGGGGCATCGATAACTATGGCGACGCCTCAACTCCAATTTTTGGCACCCAAATAGAAGCATTCCAAGGCCATCATAAGCGGCCATGGACAATCACAAAGCGTCAATTTGCCAACAATCTGTATGCTTTGGCACGAGCTGTGACTTTCACTGTACTTCCTATAACCCTTGCATTTAATGACCCTATTCTTCATGGTTTCGTTGCCGTATGCTCAGGCTGTATAATGTTCAGCCAACAGTTCCATGCATGGGCTCACGACAAAAAGAGTCAACTACCACCACTCGTAGTAGCAATGCAAGATGCTGGCCTGCTAGTGTCGCGATTGCAGCATTCTGCACATCATCGTCCACCCTATAACAACAACTACTGCATAGTAAGCGGAATTTGGAATAAGCTTTTAGATGAAACAAGGGTTTTTGAGGCATTAGAGATGATCCTGTTCTTCAAGCTTGGGGTGAGACCAAGATCTTGGACCGAGCCTCATTCCGAATGGACAGAAGAGACTGAGATTATAGAACAAACTACAGCATATTAA

Coding sequence (CDS)

ATGTCGATCTTAGTTCAAAACAAGTTCCTATTGAGCTCCAAAACACATGTTAGCCTGTGGAGCCCTGCGATTCAACGCAAATTTGTCTGCTGCATAGCCAATACCACAGTCAATGTTGCTGCTGCTACCACCGCCGCCGCCAAACCCAAGCACGGTGATGGCCAACTAGTCATTGAACGAGAGCCTTTATCTCCTACTACACCTGTATTGCCCAATTCCTCCACCTATTATTCTGTTCTTAATGATCCCAGTTTACAATCGACCTGGAGTCATCGTGTGTGGGTGACAAGTGGGTGCACCATAGTGCTAATCTCTTTAGCAAAATCAATAGCAGGTGCAGCCAGTACACATACCTGGCTTGAGCCTGCTTTAGCAGCATTGGTAGGCTACATTTTAGCTGACCTTGGATCTGGGGTTTACCATTGGGGCATCGATAACTATGGCGACGCCTCAACTCCAATTTTTGGCACCCAAATAGAAGCATTCCAAGGCCATCATAAGCGGCCATGGACAATCACAAAGCGTCAATTTGCCAACAATCTGTATGCTTTGGCACGAGCTGTGACTTTCACTGTACTTCCTATAACCCTTGCATTTAATGACCCTATTCTTCATGGTTTCGTTGCCGTATGCTCAGGCTGTATAATGTTCAGCCAACAGTTCCATGCATGGGCTCACGACAAAAAGAGTCAACTACCACCACTCGTAGTAGCAATGCAAGATGCTGGCCTGCTAGTGTCGCGATTGCAGCATTCTGCACATCATCGTCCACCCTATAACAACAACTACTGCATAGTAAGCGGAATTTGGAATAAGCTTTTAGATGAAACAAGGGTTTTTGAGGCATTAGAGATGATCCTGTTCTTCAAGCTTGGGGTGAGACCAAGATCTTGGACCGAGCCTCATTCCGAATGGACAGAAGAGACTGAGATTATAGAACAAACTACAGCATATTAA

Protein sequence

MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTEPHSEWTEETEIIEQTTAY

Homology

[Close]

BLAST of Carg00063 vs. NCBI nr
Match: KAG6600772.1 (Fatty acid desaturase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031409.1 Fatty acid desaturase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 653.7 bits (1685), Expect = 8.3e-184
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           PHSEWTEETEIIEQTTAY
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Carg00063 vs. NCBI nr
Match: XP_022943161.1 (fatty acid desaturase 4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 651.7 bits (1680), Expect = 3.2e-183
Identity = 317/318 (99.69%), Postives = 317/318 (99.69%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGD STPIFGTQIEAFQGHHKRPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDPSTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           PHSEWTEETEIIEQTTAY
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Carg00063 vs. NCBI nr
Match: XP_022976256.1 (fatty acid desaturase 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 645.6 bits (1664), Expect = 2.3e-181
Identity = 313/318 (98.43%), Postives = 316/318 (99.37%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSILVQNKFLLSSKTHVSLWSPA+ RKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAAS+HTWL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTFTVLPITLA NDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDET+VFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           PHSEWTEETEIIEQTTAY
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Carg00063 vs. NCBI nr
Match: XP_023515872.1 (fatty acid desaturase 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643.3 bits (1658), Expect = 1.1e-180
Identity = 313/318 (98.43%), Postives = 315/318 (99.06%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSILVQNKFLLSSKTHVSLWSPAI RKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAIPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAAS+HTWL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTFTVLPITLA NDPILHG VAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLASNDPILHGLVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDET+VFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           PHSEWTEETEIIEQTTAY
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Carg00063 vs. NCBI nr
Match: XP_038905900.1 (fatty acid desaturase 4, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 546.2 bits (1406), Expect = 1.9e-151
Identity = 265/318 (83.33%), Postives = 285/318 (89.62%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSIL QNKFLLSS++HV+  S  I R+   CIAN+TVNVAA  T    PKH DGQL+IER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVFYCIANSTVNVAAGAT-TTNPKHADGQLLIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           E +SPT   L NSSTYYSVLNDPSL+STWSHR WV SGCT VL+SLAKSI GAAS+H WL
Sbjct: 61  EVISPTPLTLVNSSTYYSVLNDPSLRSTWSHRAWVASGCTTVLVSLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYG+ STPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGNVSTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           +YALARAVTF VLP++LAF+DPILHGFV VCSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 MYALARAVTFAVLPVSLAFDDPILHGFVGVCSGCIMFSQQFHAWAHGKKSELPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSR QHSAHHRPPYNNNYCIVSGIWNK+LDET+VFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRRQHSAHHRPPYNNNYCIVSGIWNKILDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           P+S WTEETEIIEQTTA+
Sbjct: 301 PNSAWTEETEIIEQTTAH 317

[Close]

BLAST of Carg00063 vs. ExPASy Swiss-Prot
Match: Q9SZ42 (Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-104
Identity = 178/283 (62.90%), Postives = 218/283 (77.03%), Query Frame = 0


Query: 32  IANTTVNVAAA---TTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQST 91
           +   TVN+  +   +  + KP+    +LV+E+   SP             + NDP+L+ST
Sbjct: 48  VEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP------------PLSNDPTLKST 107

Query: 92  WSHRVWVTSGCTIVLISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYG 151
           W+HR+WV +GCT + +SLAKS+ G   +H  LEPALA   GYILADLGSGVYHW IDNYG
Sbjct: 108 WTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGVYHWAIDNYG 167

Query: 152 DASTPIFGTQIEAFQGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFV 211
           D STP+ GTQIEAFQGHHK PWTIT+RQFANNL+ALA+ +TFTVLP+ LAFNDP+ HGFV
Sbjct: 168 DESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAFNDPVFHGFV 227

Query: 212 AVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSG 271
              + CI+FSQQFHAWAH  KS+LPPLVVA+QD GLLVSR QH+ HHR PYNNNYCIVSG
Sbjct: 228 CTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPYNNNYCIVSG 287

Query: 272 IWNKLLDETRVFEALEMILFFKLGVRPRSWTEPHSEWTEETEI 312
            WN +LDE++VFEALEM+ +F+LGVRPRSW+EP+S+W EETEI
Sbjct: 288 AWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of Carg00063 vs. ExPASy Swiss-Prot
Match: O04584 (Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L1 PE=3 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-96
Identity = 165/276 (59.78%), Postives = 202/276 (73.19%), Query Frame = 0


Query: 35  TTVNVAAATTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVW 94
           T V V  + T    P+     LV+E+  ++P  P+  N        NDP+LQSTW+HR+W
Sbjct: 24  TRVRVTCSVT-TTNPQLNHENLVVEKRLVNP--PLSKN--------NDPTLQSTWTHRLW 83

Query: 95  VTSGCTIVLISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPI 154
           V +G T +  S AKSI G   +H WL+PALA   GY+ ADLGSGVYHW IDNYG ASTPI
Sbjct: 84  VAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDNYGGASTPI 143

Query: 155 FGTQIEAFQGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGC 214
            G Q+EA QGHHK PWTITKRQFANN Y +ARA+TF VLP+ LA N+P+ H FV+  + C
Sbjct: 144 VGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHSFVSTFAFC 203

Query: 215 IMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLL 274
           I+ SQQFHAWAH  KS+LPPLV+A+QD GLLVSR  H  HH+ PYN+NYC+VSG WNK+L
Sbjct: 204 ILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVVSGAWNKVL 263

Query: 275 DETRVFEALEMILFFKLGVRPRSWTEPHSEWTEETE 311
           DE+ +F+ALEM LFF+ GVRP SW EP+S+WTEETE
Sbjct: 264 DESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

[Close]

BLAST of Carg00063 vs. ExPASy Swiss-Prot
Match: O81006 (Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4L2 PE=2 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.2e-92
Identity = 155/262 (59.16%), Postives = 198/262 (75.57%), Query Frame = 0


Query: 43  TTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIV 102
           +T  ++P H + +LV+E+  ++P             + NDP+LQSTW+HR+WV +GCT V
Sbjct: 31  STTNSQPNH-EMKLVVEQRLVNP------------PLSNDPTLQSTWTHRLWVAAGCTTV 90

Query: 103 LISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAF 162
            +S +KSI GA  +H WLEP+LA   GYILADLGSGVYHW  DNYGD STP+ G  IE  
Sbjct: 91  FVSFSKSIIGAFGSHLWLEPSLAGFAGYILADLGSGVYHWATDNYGDESTPLVGIHIEDS 150

Query: 163 QGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFH 222
           Q HHK PWTITKRQFANNL+ +AR  T  VLP+ LAF+D ++HGFV++ + C++F Q FH
Sbjct: 151 QDHHKCPWTITKRQFANNLHFMARGTTLIVLPLDLAFDDHVVHGFVSMFAFCVLFCQLFH 210

Query: 223 AWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEA 282
           AWAH  KS+LPPLVV +QD GLLVSR+ H  HHR PYNNNYC+VSG+WNK+LDE+ VF+A
Sbjct: 211 AWAHGTKSKLPPLVVGLQDIGLLVSRIHHMNHHRAPYNNNYCVVSGVWNKVLDESNVFKA 270

Query: 283 LEMILFFKLGVRPRSWTEPHSE 305
           +EM+L+ +LGVRPRSWTEP+ E
Sbjct: 271 MEMVLYIQLGVRPRSWTEPNYE 279

[Close]

BLAST of Carg00063 vs. ExPASy Swiss-Prot
Match: Q9AE87 (Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.2e-17
Identity = 62/199 (31.16%), Postives = 87/199 (43.72%), Query Frame = 0


Query: 104 ISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAF 163
           ++L   + G     TWL  + A L+GY+ AD  SG  HW  D +G    P+ G   I  F
Sbjct: 42  VALVYRLWGTPYAGTWLLLS-AVLLGYLAADFVSGFVHWMGDTWGSTEMPVLGKALIRPF 101

Query: 164 QGHHKRPWTITKRQFA-----NNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMF 223
           + HH     IT+  F      N L +L  A+    LP++          F+      +M 
Sbjct: 102 REHHVDEKAITRHDFVETNGNNCLISLPVAIIALCLPMS-GPGWVFCASFLGAMIFWVMA 161

Query: 224 SQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDET 283
           + QFH W+H      P LV  +Q   L++    H  HH  PYN  YCI  G  NK L   
Sbjct: 162 TNQFHKWSH--MDSPPALVGFLQRVHLILPPDHHRIHHTKPYNKYYCITVGWMNKPLTMV 221

Query: 284 RVFEALEMILFFKLGVRPR 297
             F   E ++ +  G+ PR
Sbjct: 222 HFFPTAERLITWATGLLPR 236

[Close]

BLAST of Carg00063 vs. ExPASy Swiss-Prot
Match: Q5UR78 (Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L630 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.2e-13
Identity = 49/181 (27.07%), Postives = 82/181 (45.30%), Query Frame = 0


Query: 118 TWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFG-TQIEAFQGHHKRPWTITKRQ 177
           T ++  +  ++GY+L+D   G+YHW  D Y    TPI G T I   + HH RP  + +  
Sbjct: 4   TIIDKFIQIVLGYLLSDFIMGIYHWIKDTYFSPFTPIIGKTFIWGSRLHHVRPRYVLEFT 63

Query: 178 FANNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLV 237
             + +   A+     + P+   F    L  F+      I  +   H + H+   + P   
Sbjct: 64  DKDLIIDSAKWTLSWIGPLFFWFG---LTPFLVTMFIMISLNDVIHKYTHEIDHERPMWA 123

Query: 238 VAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPR 297
             +Q  G   S  +H  HH  P+  NYC V+   N  L++  ++  LE  + +  GV+PR
Sbjct: 124 TILQRIGFFQSHDEHHLHHIAPHEINYCPVTPYVNIWLEKINLWRKLESFVEYLTGVKPR 181

[Close]

BLAST of Carg00063 vs. ExPASy TrEMBL
Match: A0A6J1FQY8 (fatty acid desaturase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447974 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.5e-183
Identity = 317/318 (99.69%), Postives = 317/318 (99.69%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGD STPIFGTQIEAFQGHHKRPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDPSTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           PHSEWTEETEIIEQTTAY
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Carg00063 vs. ExPASy TrEMBL
Match: A0A6J1IGF1 (fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111476701 PE=3 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.1e-181
Identity = 313/318 (98.43%), Postives = 316/318 (99.37%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSILVQNKFLLSSKTHVSLWSPA+ RKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER
Sbjct: 1   MSILVQNKFLLSSKTHVSLWSPAVPRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAAS+HTWL
Sbjct: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASSHTWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTFTVLPITLA NDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFTVLPITLAVNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDET+VFEALEMILFFKLGVRPRSWTE
Sbjct: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETKVFEALEMILFFKLGVRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           PHSEWTEETEIIEQTTAY
Sbjct: 301 PHSEWTEETEIIEQTTAY 318

[Close]

BLAST of Carg00063 vs. ExPASy TrEMBL
Match: A0A0A0L6B9 (TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G077710 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.5e-149
Identity = 261/318 (82.08%), Postives = 281/318 (88.36%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSIL QNKFLLSS++HV+  S  I R+ V CIANTTVN       A  PKH DGQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPIPRRVVYCIANTTVN-----GVATSPKHADGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           E +SPT     NSSTYYSVLNDPSL+STWSHR WV  GCT VLISLAKSI GAAS+H WL
Sbjct: 61  EVISPTPLTFVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTF VLP++LA +DPILHGFV +CSGCIMFSQQFHAWAH KKS+LPPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAIDDPILHGFVGMCSGCIMFSQQFHAWAHSKKSELPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           +AGLLVSR QHS HHRPPYNNNYCIVSGIWNKLLDET++FEALEMIL+FKLG+RPRSWTE
Sbjct: 241 NAGLLVSRTQHSTHHRPPYNNNYCIVSGIWNKLLDETKLFEALEMILYFKLGLRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Carg00063 vs. ExPASy TrEMBL
Match: A0A6J1DD70 (fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111019014 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.6e-148
Identity = 263/317 (82.97%), Postives = 281/317 (88.64%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSIL QNKFLLSS+T V+  SP I RK V C+AN TV+VAAA     KPKH DGQLVI+R
Sbjct: 1   MSILAQNKFLLSSRTRVNPLSPPIPRKIVYCVAN-TVDVAAAGATTTKPKHADGQLVIKR 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           E +SP    L  +STY+ VLNDPSL+STW HR WV SGCT VLISLAKSI GAAS+H WL
Sbjct: 61  EFVSPPL-TLGKASTYHYVLNDPSLRSTWGHRAWVASGCTTVLISLAKSIVGAASSHIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EP LAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPTLAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTF VLPI LAF DPILHGFV+VCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ
Sbjct: 181 LYALARAVTFVVLPINLAFGDPILHGFVSVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSR+QHSAHHRPPYNNNYCIVSGIWN+LLDET+VFEA EMILFFKLGVRPRSW+E
Sbjct: 241 DAGLLVSRMQHSAHHRPPYNNNYCIVSGIWNQLLDETKVFEAFEMILFFKLGVRPRSWSE 300

Query: 301 PHSEWTEETEIIEQTTA 318
           P++EWTEETE +EQTTA
Sbjct: 301 PNTEWTEETETVEQTTA 315

[Close]

BLAST of Carg00063 vs. ExPASy TrEMBL
Match: A0A5A7SXQ7 (Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold925G00380 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.5e-146
Identity = 257/318 (80.82%), Postives = 280/318 (88.05%), Query Frame = 0


Query: 1   MSILVQNKFLLSSKTHVSLWSPAIQRKFVCCIANTTVNVAAATTAAAKPKHGDGQLVIER 60
           MSIL QNKFLLSS++HV+  S    R+ V CIANTTVN  A ++     KHGDGQLVIER
Sbjct: 1   MSILAQNKFLLSSRSHVNPCSTPTPRRVVYCIANTTVNGVATSS-----KHGDGQLVIER 60

Query: 61  EPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIVLISLAKSIAGAASTHTWL 120
           E +SP+     NSSTYYSVLNDPSL+STWSHR WV  GCT VLISLAKSI GAAS+  WL
Sbjct: 61  EVISPSPLTSVNSSTYYSVLNDPSLRSTWSHRAWVAGGCTTVLISLAKSIVGAASSRIWL 120

Query: 121 EPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAFQGHHKRPWTITKRQFANN 180
           EPALAALVGYILADLGSGVYHWGIDNYG ASTPIFGTQIEAFQGHHK PWTITKRQFANN
Sbjct: 121 EPALAALVGYILADLGSGVYHWGIDNYGGASTPIFGTQIEAFQGHHKWPWTITKRQFANN 180

Query: 181 LYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQ 240
           LYALARAVTF VLP++LAF+DP LHGFV VCSGCIMFSQQFHAWAH KKS++PPLVVAMQ
Sbjct: 181 LYALARAVTFAVLPVSLAFDDPTLHGFVGVCSGCIMFSQQFHAWAHGKKSEIPPLVVAMQ 240

Query: 241 DAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEALEMILFFKLGVRPRSWTE 300
           DAGLLVSR QH+ HHRPPYNNNYCIVSGIWN+LLDET+VFEALEMIL+FKLG+RPRSWTE
Sbjct: 241 DAGLLVSRTQHATHHRPPYNNNYCIVSGIWNQLLDETKVFEALEMILYFKLGLRPRSWTE 300

Query: 301 PHSEWTEETEIIEQTTAY 319
           P+SEWTEETEI+EQTTA+
Sbjct: 301 PNSEWTEETEILEQTTAH 313

[Close]

BLAST of Carg00063 vs. TAIR 10
Match: AT4G27030.1 (fatty acid desaturase A )

HSP 1 Score: 380.9 bits (977), Expect = 9.7e-106
Identity = 178/283 (62.90%), Postives = 218/283 (77.03%), Query Frame = 0


Query: 32  IANTTVNVAAA---TTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQST 91
           +   TVN+  +   +  + KP+    +LV+E+   SP             + NDP+L+ST
Sbjct: 48  VEQRTVNLPLSNDQSLQSTKPRPNREKLVVEQRLASP------------PLSNDPTLKST 107

Query: 92  WSHRVWVTSGCTIVLISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYG 151
           W+HR+WV +GCT + +SLAKS+ G   +H  LEPALA   GYILADLGSGVYHW IDNYG
Sbjct: 108 WTHRLWVAAGCTTLFVSLAKSVIGGFDSHLCLEPALAGYAGYILADLGSGVYHWAIDNYG 167

Query: 152 DASTPIFGTQIEAFQGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFV 211
           D STP+ GTQIEAFQGHHK PWTIT+RQFANNL+ALA+ +TFTVLP+ LAFNDP+ HGFV
Sbjct: 168 DESTPVVGTQIEAFQGHHKWPWTITRRQFANNLHALAQVITFTVLPLDLAFNDPVFHGFV 227

Query: 212 AVCSGCIMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSG 271
              + CI+FSQQFHAWAH  KS+LPPLVVA+QD GLLVSR QH+ HHR PYNNNYCIVSG
Sbjct: 228 CTFAFCILFSQQFHAWAHGTKSKLPPLVVALQDMGLLVSRRQHAEHHRAPYNNNYCIVSG 287

Query: 272 IWNKLLDETRVFEALEMILFFKLGVRPRSWTEPHSEWTEETEI 312
            WN +LDE++VFEALEM+ +F+LGVRPRSW+EP+S+W EETEI
Sbjct: 288 AWNNVLDESKVFEALEMVFYFQLGVRPRSWSEPNSDWIEETEI 318

[Close]

BLAST of Carg00063 vs. TAIR 10
Match: AT1G62190.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 353.2 bits (905), Expect = 2.2e-97
Identity = 165/276 (59.78%), Postives = 202/276 (73.19%), Query Frame = 0


Query: 35  TTVNVAAATTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVW 94
           T V V  + T    P+     LV+E+  ++P  P+  N        NDP+LQSTW+HR+W
Sbjct: 24  TRVRVTCSVT-TTNPQLNHENLVVEKRLVNP--PLSKN--------NDPTLQSTWTHRLW 83

Query: 95  VTSGCTIVLISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPI 154
           V +G T +  S AKSI G   +H WL+PALA   GY+ ADLGSGVYHW IDNYG ASTPI
Sbjct: 84  VAAGSTTIFASFAKSIIGGFGSHLWLQPALACYAGYVFADLGSGVYHWAIDNYGGASTPI 143

Query: 155 FGTQIEAFQGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGC 214
            G Q+EA QGHHK PWTITKRQFANN Y +ARA+TF VLP+ LA N+P+ H FV+  + C
Sbjct: 144 VGAQLEASQGHHKYPWTITKRQFANNSYTIARAITFIVLPLNLAINNPLFHSFVSTFAFC 203

Query: 215 IMFSQQFHAWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLL 274
           I+ SQQFHAWAH  KS+LPPLV+A+QD GLLVSR  H  HH+ PYN+NYC+VSG WNK+L
Sbjct: 204 ILLSQQFHAWAHGTKSKLPPLVMALQDMGLLVSRKDHPGHHQAPYNSNYCVVSGAWNKVL 263

Query: 275 DETRVFEALEMILFFKLGVRPRSWTEPHSEWTEETE 311
           DE+ +F+ALEM LFF+ GVRP SW EP+S+WTEETE
Sbjct: 264 DESNLFKALEMALFFQFGVRPNSWNEPNSDWTEETE 288

[Close]

BLAST of Carg00063 vs. TAIR 10
Match: AT2G22890.1 (Kua-ubiquitin conjugating enzyme hybrid localisation domain )

HSP 1 Score: 341.3 bits (874), Expect = 8.5e-94
Identity = 155/262 (59.16%), Postives = 198/262 (75.57%), Query Frame = 0


Query: 43  TTAAAKPKHGDGQLVIEREPLSPTTPVLPNSSTYYSVLNDPSLQSTWSHRVWVTSGCTIV 102
           +T  ++P H + +LV+E+  ++P             + NDP+LQSTW+HR+WV +GCT V
Sbjct: 31  STTNSQPNH-EMKLVVEQRLVNP------------PLSNDPTLQSTWTHRLWVAAGCTTV 90

Query: 103 LISLAKSIAGAASTHTWLEPALAALVGYILADLGSGVYHWGIDNYGDASTPIFGTQIEAF 162
            +S +KSI GA  +H WLEP+LA   GYILADLGSGVYHW  DNYGD STP+ G  IE  
Sbjct: 91  FVSFSKSIIGAFGSHLWLEPSLAGFAGYILADLGSGVYHWATDNYGDESTPLVGIHIEDS 150

Query: 163 QGHHKRPWTITKRQFANNLYALARAVTFTVLPITLAFNDPILHGFVAVCSGCIMFSQQFH 222
           Q HHK PWTITKRQFANNL+ +AR  T  VLP+ LAF+D ++HGFV++ + C++F Q FH
Sbjct: 151 QDHHKCPWTITKRQFANNLHFMARGTTLIVLPLDLAFDDHVVHGFVSMFAFCVLFCQLFH 210

Query: 223 AWAHDKKSQLPPLVVAMQDAGLLVSRLQHSAHHRPPYNNNYCIVSGIWNKLLDETRVFEA 282
           AWAH  KS+LPPLVV +QD GLLVSR+ H  HHR PYNNNYC+VSG+WNK+LDE+ VF+A
Sbjct: 211 AWAHGTKSKLPPLVVGLQDIGLLVSRIHHMNHHRAPYNNNYCVVSGVWNKVLDESNVFKA 270

Query: 283 LEMILFFKLGVRPRSWTEPHSE 305
           +EM+L+ +LGVRPRSWTEP+ E
Sbjct: 271 MEMVLYIQLGVRPRSWTEPNYE 279

The following BLAST results are available for this feature:

BLAST of Carg00063 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6600772.1	8.3e-184	100.00	Fatty acid desaturase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022943161.1	3.2e-183	99.69	fatty acid desaturase 4, chloroplastic [Cucurbita moschata]	[more]
XP_022976256.1	2.3e-181	98.43	fatty acid desaturase 4, chloroplastic [Cucurbita maxima]	[more]
XP_023515872.1	1.1e-180	98.43	fatty acid desaturase 4, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_038905900.1	1.9e-151	83.33	fatty acid desaturase 4, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Carg00063 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZ42	1.4e-104	62.90	Fatty acid desaturase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD4 P...	[more]
O04584	3.0e-96	59.78	Fatty acid desaturase 4-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
O81006	1.2e-92	59.16	Fatty acid desaturase 4-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9AE87	2.2e-17	31.16	Plasmanylethanolamine desaturase OS=Myxococcus xanthus OX=34 GN=carF PE=1 SV=1	[more]
Q5UR78	3.2e-13	27.07	Probable fatty acid desaturase MIMI_L630 OS=Acanthamoeba polyphaga mimivirus OX=...	[more]

BLAST of Carg00063 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQY8	1.5e-183	99.69	fatty acid desaturase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1IGF1	1.1e-181	98.43	fatty acid desaturase 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114767...	[more]
A0A0A0L6B9	2.5e-149	82.08	TMEM189_B_dmain domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G07...	[more]
A0A6J1DD70	1.6e-148	82.97	fatty acid desaturase 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A5A7SXQ7	1.5e-146	80.82	Fatty acid desaturase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Carg00063 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27030.1	9.7e-106	62.90	fatty acid desaturase A	[more]
AT1G62190.1	2.2e-97	59.78	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]
AT2G22890.1	8.5e-94	59.16	Kua-ubiquitin conjugating enzyme hybrid localisation domain	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019547	B domain of TMEM189, localisation domain	PFAM	PF10520	TMEM189_B_dmain	coord: 130..298 e-value: 1.9E-64 score: 216.9
None	No IPR available	PANTHER	PTHR48140	FAMILY NOT NAMED	coord: 1..314

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg00063-RA	Carg00063-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006631	fatty acid metabolic process
cellular_component	GO:0016021	integral component of membrane