CaUC09G176480 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC09G176480
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A
Location	Ciama_Chr09: 35709709 .. 35710505 (-)
RNA-Seq Expression	CaUC09G176480
Synteny	CaUC09G176480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACCGAAATTAATGGGCCGGGGCCCAAACATCCGTTTGAGAAAGCTGCATAGAATAGAAACGAGGTTTTTTCCAAGTCTACTGCATCTCGAAGAATTTTGTCGCCGGATCTTCTTTTTGCCTTCGTGTTTCTCTCTCAATTAATCAGATCGGAAGGTACAGTCGTCTTCCTCTTTTCCTCTCAAAATTTCATTAGAAGATTTTATCAAATTTTTCTTAGTTCAGCTGTAGAATTTTCGTTTTTCTTTTTACCAAATTTCGATTCTTAATCTCTAGACTTGTGAATCGCTCTTCCAAACCGTGTGATTAGGTTACAATGTCTAGTTTTTGAAGAATTTGAAATGTTCAGTTTTCTGCAATGAAATTTGAATCAGTTGTAGGCGATCGAAGATTTTAATTTCCGTTGTATTGTTGCGCAGCGCAGATGATTGACGATCAGGAGTTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCTTTAGTGATTGCTTACCATTACGTTACGGCTGATCCGAAATATGAAGGCAATTGAGATCTCGTTGATTCAGATGCCTAGATTGATCGGTAAAATGCACTACTGTTAGATGTATACCGTTTTTGATCTGTATGTTAGGTTTTCCTTTTCTTCTGTGAGAATTTTCTCCCGGCAACAATCAATGTAAGCAACCTTTCACTATGAAATCGTAACTTATTCTCATTATTTTTCATGGCGCTTTGATTTATCTCATATTATTTACGCATTTGATTGAATTTCTTCATACGGCCTTGAGTTCCTTCAAATTTGTGTT

mRNA sequence

ATGAAACCGAAATTAATGGGCCGGGGCCCAAACATCCGTTTGAGAAAGCTGCATAGAATAGAAACGAGACTTGTGAATCGCTCTTCCAAACCGTCGCAGATGATTGACGATCAGGAGTTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCTTTAGTGATTGCTTACCATTACGTTACGGCTGATCCGAAATATGAAGGCAATTGAGATCTCGTTGATTCAGATGCCTAGATTGATCGGTAAAATGCACTACTGTTAGATGTATACCGTTTTTGATCTGTATGTTAGGTTTTCCTTTTCTTCTGTGAGAATTTTCTCCCGGCAACAATCAATGTAAGCAACCTTTCACTATGAAATCGTAACTTATTCTCATTATTTTTCATGGCGCTTTGATTTATCTCATATTATTTACGCATTTGATTGAATTTCTTCATACGGCCTTGAGTTCCTTCAAATTTGTGTT

Coding sequence (CDS)

ATGAAACCGAAATTAATGGGCCGGGGCCCAAACATCCGTTTGAGAAAGCTGCATAGAATAGAAACGAGACTTGTGAATCGCTCTTCCAAACCGTCGCAGATGATTGACGATCAGGAGTTAGGTTTTCTTGCCAATTTTCTCGGCATTTTTATCTTTGCTTTAGTGATTGCTTACCATTACGTTACGGCTGATCCGAAATATGAAGGCAATTGA

Protein sequence

MKPKLMGRGPNIRLRKLHRIETRLVNRSSKPSQMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN

Homology

[Close]

BLAST of CaUC09G176480 vs. NCBI nr
Match: KCW49626.1 (hypothetical protein EUGRSUZ_K03148, partial [Eucalyptus grandis])

HSP 1 Score: 80.9 bits (198), Expect = 4.9e-12
Identity = 37/44 (84.09%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 27 RSSKPSQMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          RS   ++MIDD++LGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 26 RSGAVAKMIDDRDLGFLANFLGIFIFALVIAYHYVTADPKYEGN 69

[Close]

BLAST of CaUC09G176480 vs. NCBI nr
Match: XP_008463467.1 (PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis melo] >XP_008463468.1 PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis melo] >XP_022939332.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucurbita moschata] >XP_022993977.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucurbita maxima] >XP_023551013.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucurbita pepo subsp. pepo] >XP_038890715.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Benincasa hispida] >XP_038890716.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Benincasa hispida] >XP_038890717.1 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Benincasa hispida])

HSP 1 Score: 80.1 bits (196), Expect = 8.4e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. NCBI nr
Match: TXG58580.1 (hypothetical protein EZV62_016409 [Acer yangbiense])

HSP 1 Score: 79.7 bits (195), Expect = 1.1e-11
Identity = 36/39 (92.31%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 32 SQMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          S MIDDQ+LGF+ANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 28 SHMIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYEGN 66

[Close]

BLAST of CaUC09G176480 vs. NCBI nr
Match: XP_004142744.1 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis sativus])

HSP 1 Score: 79.7 bits (195), Expect = 1.1e-11
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          MIDDQELGFLANFLGIF+FALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFVFALVIAYHYVTADPKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. NCBI nr
Match: OMO96944.1 (Oligosaccaryltransferase [Corchorus olitorius])

HSP 1 Score: 79.0 bits (193), Expect = 1.9e-11
Identity = 36/43 (83.72%), Postives = 39/43 (90.70%), Query Frame = 0


Query: 28  SSKPSQMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
           S +  +MIDDQ+LGFLANFLGIFIFALVIAYHYVTADP YEGN
Sbjct: 64  SPRSVKMIDDQQLGFLANFLGIFIFALVIAYHYVTADPNYEGN 106

[Close]

BLAST of CaUC09G176480 vs. ExPASy Swiss-Prot
Match: Q9LHK3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Arabidopsis thaliana OX=3702 GN=OST4A PE=3 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.7e-12
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 69
          MIDDQ+LGF+ANFLGIFIFALVIAYHYVTADPKYE
Sbjct: 1  MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

[Close]

BLAST of CaUC09G176480 vs. ExPASy Swiss-Prot
Match: C7J4U3 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Oryza sativa subsp. japonica OX=39947 GN=OST4A PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 8.7e-12
Identity = 31/37 (83.78%), Postives = 33/37 (89.19%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          M DDQ+LGF ANFLGIFIF LVIAYH+V ADPKYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. ExPASy Swiss-Prot
Match: C7J0R5 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Oryza sativa subsp. japonica OX=39947 GN=OST4B PE=3 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 2.1e-10
Identity = 29/37 (78.38%), Postives = 32/37 (86.49%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          M DDQ+LGF ANFLGIFIF LV+AYH+V AD KYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVMAYHFVMADVKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. ExPASy Swiss-Prot
Match: Q9SF57 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Arabidopsis thaliana OX=3702 GN=OST4C PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.4e-09
Identity = 28/35 (80.00%), Postives = 31/35 (88.57%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 69
          M DDQ+LGF ANFLGIFIF LVIAYH+V ADPK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFILVIAYHFVMADPKFE 35

[Close]

BLAST of CaUC09G176480 vs. ExPASy Swiss-Prot
Match: Q8L986 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Arabidopsis thaliana OX=3702 GN=OST4B PE=3 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.3e-09
Identity = 26/35 (74.29%), Postives = 31/35 (88.57%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 69
          M DDQ+LGF ANFLGIFIF +VIAYH+V A+PK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

[Close]

BLAST of CaUC09G176480 vs. ExPASy TrEMBL
Match: A0A059A7I3 (Uncharacterized protein (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K03148 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.4e-12
Identity = 37/44 (84.09%), Postives = 41/44 (93.18%), Query Frame = 0


Query: 27 RSSKPSQMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          RS   ++MIDD++LGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 26 RSGAVAKMIDDRDLGFLANFLGIFIFALVIAYHYVTADPKYEGN 69

[Close]

BLAST of CaUC09G176480 vs. ExPASy TrEMBL
Match: A0A1S3CJC4 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cucumis melo OX=3656 GN=LOC103501634 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.0e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. ExPASy TrEMBL
Match: A0A6J1JUF0 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cucurbita maxima OX=3661 GN=LOC111489813 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.0e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. ExPASy TrEMBL
Match: A0A6J1FGH7 (dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cucurbita moschata OX=3662 GN=LOC111445281 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.0e-12
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 1  MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 37

[Close]

BLAST of CaUC09G176480 vs. ExPASy TrEMBL
Match: A0A5C7HNE0 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_016409 PE=3 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 5.3e-12
Identity = 36/39 (92.31%), Postives = 38/39 (97.44%), Query Frame = 0


Query: 32 SQMIDDQELGFLANFLGIFIFALVIAYHYVTADPKYEGN 71
          S MIDDQ+LGF+ANFLGIFIFALVIAYHYVTADPKYEGN
Sbjct: 28 SHMIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYEGN 66

[Close]

BLAST of CaUC09G176480 vs. TAIR 10
Match: AT3G12587.1 (Oligosaccaryltransferase )

HSP 1 Score: 72.8 bits (177), Expect = 1.2e-13
Identity = 33/35 (94.29%), Postives = 35/35 (100.00%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 69
          MIDDQ+LGF+ANFLGIFIFALVIAYHYVTADPKYE
Sbjct: 1  MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

[Close]

BLAST of CaUC09G176480 vs. TAIR 10
Match: AT5G02502.1 (Oligosaccaryltransferase )

HSP 1 Score: 61.2 bits (147), Expect = 3.7e-10
Identity = 26/35 (74.29%), Postives = 31/35 (88.57%), Query Frame = 0


Query: 34 MIDDQELGFLANFLGIFIFALVIAYHYVTADPKYE 69
          M DDQ+LGF ANFLGIFIF +VIAYH+V A+PK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

The following BLAST results are available for this feature:

BLAST of CaUC09G176480 vs. NCBI nr


Match Name	E-value	Identity	Description
KCW49626.1	4.9e-12	84.09	hypothetical protein EUGRSUZ_K03148, partial [Eucalyptus grandis]	[more]
XP_008463467.1	8.4e-12	100.00	PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subuni...	[more]
TXG58580.1	1.1e-11	92.31	hypothetical protein EZV62_016409 [Acer yangbiense]	[more]
XP_004142744.1	1.1e-11	97.30	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucum...	[more]
OMO96944.1	1.9e-11	83.72	Oligosaccaryltransferase [Corchorus olitorius]	[more]

BLAST of CaUC09G176480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHK3	1.7e-12	94.29	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ara...	[more]
C7J4U3	8.7e-12	83.78	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ory...	[more]
C7J0R5	2.1e-10	78.38	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ory...	[more]
Q9SF57	1.4e-09	80.00	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Ara...	[more]
Q8L986	5.3e-09	74.29	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ara...	[more]

BLAST of CaUC09G176480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A059A7I3	2.4e-12	84.09	Uncharacterized protein (Fragment) OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_K03...	[more]
A0A1S3CJC4	4.0e-12	100.00	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cuc...	[more]
A0A6J1JUF0	4.0e-12	100.00	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cuc...	[more]
A0A6J1FGH7	4.0e-12	100.00	dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Cuc...	[more]
A0A5C7HNE0	5.3e-12	92.31	Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_016409 PE=3 SV=1	[more]

BLAST of CaUC09G176480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12587.1	1.2e-13	94.29	Oligosaccaryltransferase	[more]
AT5G02502.1	3.7e-10	74.29	Oligosaccaryltransferase	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018943	Oligosaccaryltransferase	PFAM	PF10215	Ost4	coord: 34..66 e-value: 2.0E-15 score: 56.3
IPR044165	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4, plant	PANTHER	PTHR28677	DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 4A-RELATED	coord: 17..70
IPR036330	Oligosaccharyltransferase subunit ost4p superfamily	SUPERFAMILY	103464	Oligosaccharyltransferase subunit ost4p	coord: 34..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC09G176480.1	CaUC09G176480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016740	transferase activity