CaUC06G109380 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC06G109380
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Descriptioncytochrome b5
LocationCiama_Chr06: 3440837 .. 3444034 (+)
RNA-Seq ExpressionCaUC06G109380
SyntenyCaUC06G109380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAACCCATGGGCTTTGACGGTTGGACCCAACATTTTTAGCCCAAAATAAAATTAAAGAAACGAAAAAAATAAAATAAAATAAAAATTGTGGATGTGACCATTTAGTCGTTTTTCACGTGGATCGGCCTCAGATCTGGTCCACGTTTATTCACCAACTAATTATTAAAATAAATCATATAAAATTTATATTATTTAAATATACTTTATACTTTTAAAATGACATTACTCTTACATAAAACAGCAAGATATAAGCTTTATTCAATAATAGCACATGCCCATTTTTTTTCTCAAACCCATACTTCTTTTCAATGGAACTATATTCAATTTAAAATTGAATTAATAAAAGTCTTATGTTTACAACTTAACATTTATATATTTATATATTTAAGACACCAACAACTAAAATAAATACACCCACAAAGGGAACATTTTTTTTTTGAAAGAGCAATCAATTAGAAGAAATCTTGTACTATTCTCTCTCAAGTAAGTGACTCTTAAGCTTTGATTCTTTTCATTTTGATTTCTCTTCTACTTCTTCTTCTGTTTGTGGTCTGAAATATTCAATCTCTCTTCAAGAAAGTTGCAGAGAAAAGTTGATGGAAATGGATTCAAATTCTAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAAGATTGCTGGCTTATAATCAATGGTTATGTATGTCCTTTCCTTTAATTTTCTACTTTTTAACTCTCTCTCTCTTAGAATTGATTGGTTAATGGAGTTTCCAAAACAAATCAACTAAAGTTATTTTAGATTTTTTTTTTTTTAATTTATTTTTCACTTCATATCAATCCAATTAAAATGTTTTATGATTGTATCTGTTATGTTATGAATATACTACTTATAAAATCTCTCTAAATTCTCCATTTTTACTTTCTTTCTTAGGATATTAAGCATCTATGTAGTCTACTTACAATTTCATTTTGGTCTTTTTATATCTAAATTAAAATTTCAATTCAAAATTTTAAAAAGTTAATTGGTATTTCTTTTACATCATAAAGATTTCGAACATAACTCAACTTACATATGAATATATTTGCAATTATTTAAAAATATTATTAAATACTTAAGGTGTGTTTGGAATACATTTTCAAGTGTTTAATTTAAAAAACAAGTCATTTTGAAATAAATAATTGTTTGACAATCACTCAAAATGAATTTTGGGAGTCATTAGTGTTTTTGTTTTAAACCGTTTGTATCAAAATCGTTTAAATAACCAAAAAAAAATTTAAAAACATTTGCCTTTAATTATTTGCCGACTGTTGAACTGCAATCCCCAACAAAAATGAATGGATACCAATTGTTTATTTAATAATTTAAATGTTTACTTCCAATACTCCTTTGGTTGTTTTATATCTTTTTAAAAATTGTTTTGGTTGTACTTTTAAGAAGTTCTCTTTCTACTATTTGTTTGCAAAATTTTAAAATAACTTTTAAAAACAACAAAACATTTCGAATTTGAATAATTTTTTTAAATTACAGTGATAGATGATGATAGTCTATAAGTAATAGATTTGTTATTTTACTATATTTATAAATATTTTAACTTATTTTATTATATTTGAAAATAACGAAATTTATTTAAGTAATAGATTTGTTATTTTACTATATTTATAAATATTTTAACTTATTTTATTATATTTGAAAATAACGAAATTTATTAATTATTGGTAGTTATGTACTAAAACTATAGATGAAAAAAATGAGTTAAAAAGAAAATGAAGTAATAAAAAAAAATTACCACTATGCTAATTTATAAAAGTGGATTTGTGTTAGGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTCTTGCTACTTGCCGTCGGTGAGATGCTCTAATTAATAACTTTTTCTATTGTTTTAATTACCAAAATTGAATTGACATTGACAGTTTTAGTATACTAGTTTATATATGTTTAATAAACCTTAAAATTAGTCTTCATCGTTACTTTGAAATTCACTTTTATCTAACTTTTCAAAACAACAATTTTTTTCGAATAATCTAATACCAAATGCGTTAATGTATAATGATCTAATCTTAGAAACCAATAAACATTGAATTTCTGCTTACGTCACATAGTATTAAAGAGCCTACAACTTCTTCTCGCGATCGTGACTTCTCACCCATCATGAAATATATGAATCTCTAATGAGATTTGAATGATTTGCATCACGATTCTTTTTAAAAAAGAAAATCCTCTCATTGGCCATTAAAGGTTGCGAGAGTCCAACTATCGTGAAATTCTAATGGAGTTTGATCGGGATTCCTCAACAATCAAAAATATGAAAAATAAACTTTTGGGGATTCTAATTAATTTAAACCAGTCATGATGACAATTTAAAATTGCGACCAGGTTCCGCTAGAATTTCGCAGTGGTTGGGCACCCACAACCACGAATGAGCATCAGAGAGATTTTAAAAAAAATGCGATGTAGATTCAAATTGCATTAGGGATGATCCACATATTCTGCCATGGGTGAGAAGCTAGCCATGATCTCTATAAGAAGTTATATGCTCTTAAGTCTTAAGTAGTCTGTGACATAACAGGCTGAAGATTGGTTTCATTGGTTTCCAAACTTGGATCATTCTTCATTAGCGCATTTAGAACTAGATTATTCATACAAAAATCTATATATATATATATATATATATAATTTCTATTTTTTAAAACAAAACATAACATCAAGAAAGTTAATAGAGATTAATAATTTTTTAAAAAATTATATATACATTGACAATTGAGACTAGTGTAGGATTTATTAAAAGTATAAAGAATAAATTGAGTTTGGAGAGAGTAAAATAAATGAAACTTCTAACTTTAAGATTGATAGTACATGTATCGATATCAGCTCAATGAGCTATATTTTAAAAATGTTAGTGATAGATTTGAATATGTGCAGGAAAAGATGCAACATTTGATTTTAAGAGTGTTGGACACAGCGAATTGGCTCAAGAGAAGATGAAAAAGTATCGGATAGGGAAGATCGACATATCGACTCTCCCTAAAAAACAAGAATATGTGGAAACTACACCATATCAGCCAACTCCAAGTCTTTTTTCCTTCTCACCCATCCATGTTCTAATCCCTATTTTGCTCTTAGCCTTGGCATTTGCCTTTCCCTACTTCAAACTTAAAGCCTAA

mRNA sequence

ATGAAACCCATGGGCTTTGACGAAAGTTGCAGAGAAAAGTTGATGGAAATGGATTCAAATTCTAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAAGATTGCTGGCTTATAATCAATGGTTATGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTCTTGCTACTTGCCAGTGTTGGACACAGCGAATTGGCTCAAGAGAAGATGAAAAAGTATCGGATAGGGAAGATCGACATATCGACTCTCCCTAAAAAACAAGAATATGTGGAAACTACACCATATCAGCCAACTCCAAGTCTTTTTTCCTTCTCACCCATCCATGTTCTAATCCCTATTTTGCTCTTAGCCTTGGCATTTGCCTTTCCCTACTTCAAACTTAAAGCCTAA

Coding sequence (CDS)

ATGAAACCCATGGGCTTTGACGAAAGTTGCAGAGAAAAGTTGATGGAAATGGATTCAAATTCTAAAGTGTATTCTTTTGATGAAGTTGCAAAGCACAACCACCAGAAAGATTGCTGGCTTATAATCAATGGTTATGTGTACGATGTGACTTCATTTTTGCCGGACCATCCAGGTGGCGATGAGCTCTTGCTACTTGCCAGTGTTGGACACAGCGAATTGGCTCAAGAGAAGATGAAAAAGTATCGGATAGGGAAGATCGACATATCGACTCTCCCTAAAAAACAAGAATATGTGGAAACTACACCATATCAGCCAACTCCAAGTCTTTTTTCCTTCTCACCCATCCATGTTCTAATCCCTATTTTGCTCTTAGCCTTGGCATTTGCCTTTCCCTACTTCAAACTTAAAGCCTAA

Protein sequence

MKPMGFDESCREKLMEMDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLASVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLALAFAFPYFKLKA
Homology
BLAST of CaUC06G109380 vs. NCBI nr
Match: XP_008459741.1 (PREDICTED: cytochrome b5 [Cucumis melo])

HSP 1 Score: 229.2 bits (583), Expect = 2.2e-56
Identity = 115/131 (87.79%), Postives = 119/131 (90.84%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSKVYSFDEVAKHNHQKDCWLII+GYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSELAQEKMK YRIGKID+STLPKKQ YVE+TPYQPTPSLFSFSPIHVL+PILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

BLAST of CaUC06G109380 vs. NCBI nr
Match: XP_038875836.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 224.9 bits (572), Expect = 4.1e-55
Identity = 110/133 (82.71%), Postives = 122/133 (91.73%), Query Frame = 0

Query: 15  MEMDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA-------- 74
           M+MDS+SK+YSFDEV+KHNH +DCWLII+GYVYDVT+FLPDHPGG+ELLLLA        
Sbjct: 1   MQMDSSSKLYSFDEVSKHNHHRDCWLIISGYVYDVTAFLPDHPGGEELLLLAVGRDATFD 60

Query: 75  --SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLL 134
             SVGHSELAQEKMKKYRIGKID+STLP+KQ+YVETTPYQPTPSLFSFSPIH+LIPILLL
Sbjct: 61  FKSVGHSELAQEKMKKYRIGKIDMSTLPRKQQYVETTPYQPTPSLFSFSPIHLLIPILLL 120

Query: 135 ALAFAFPYFKLKA 138
           ALAFAFPYFKLKA
Sbjct: 121 ALAFAFPYFKLKA 133

BLAST of CaUC06G109380 vs. NCBI nr
Match: XP_004140502.1 (cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cucumis sativus])

HSP 1 Score: 214.9 bits (546), Expect = 4.3e-52
Identity = 108/131 (82.44%), Postives = 116/131 (88.55%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSKVYSFDEVAKHN QKDCWLII+GYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSELA EKMK Y+IGKID+STLP+KQ+YVE+ PYQ TPSLFSFSPIH+L+PILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

BLAST of CaUC06G109380 vs. NCBI nr
Match: XP_023000205.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 198.4 bits (503), Expect = 4.1e-47
Identity = 96/131 (73.28%), Postives = 111/131 (84.73%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSK+Y+F+EVAKHNH+KDCWLII+GYVYDVTS+LPDHPGG ELL+L           
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSE A+E MKKY+IGKID  TLP +Q+YVETTPY+PTPSL SFSP+HVLIP+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

BLAST of CaUC06G109380 vs. NCBI nr
Match: XP_022964486.1 (cytochrome b5-like [Cucurbita moschata])

HSP 1 Score: 197.2 bits (500), Expect = 9.2e-47
Identity = 96/131 (73.28%), Postives = 113/131 (86.26%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSKVY+FDEVAKHNH+KDCWLII+G+VYDVTS+LPDHPGG ELL+LA          
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSE A+E+MKKY+IGKID  TLP +Q++VETTP++PTPSL SFSP+HVLIP+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

BLAST of CaUC06G109380 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 1.9e-23
Identity = 64/136 (47.06%), Postives = 84/136 (61.76%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M S+ KV SF+EV+KHN  KDCWLII+G VYDVT F+ DHPGGDE+LL ++         
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP------TPSLFSFSPIHVLIP 136
            VGHS+ A++ M KY IG+ID S++P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

BLAST of CaUC06G109380 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 7.4e-23
Identity = 63/136 (46.32%), Postives = 82/136 (60.29%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M S  KV  F+EV++HN  KDCWLII+G VYDVT F+ DHPGGDE+LL ++         
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP------TPSLFSFSPIHVLIP 136
            VGHS+ A++ M+KY IG+ID ST+P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVPATRTYV--APVQPAYNQDKTPE-FMIKILQFLVP 120

BLAST of CaUC06G109380 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-21
Identity = 54/126 (42.86%), Postives = 79/126 (62.70%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M   +K+++  EV++HN   DCW++ING VY+VT FL DHPGGD++LL ++         
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP-----TPSLFSFSPIHVLIPI 128
            VGHSE A+E M++Y +G+ID +T+PKK +Y  T P QP       S F    +  L+P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

BLAST of CaUC06G109380 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 5.3e-21
Identity = 55/132 (41.67%), Postives = 80/132 (60.61%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M   +KV++  EV++HN+ KDCWL+I+G VYDVT FL DHPGGDE+LL A+         
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP-----TPSLFSFSPIHVLIPI 134
            VGHS  A+  + +Y +G ID +T+P K +Y  T P QP       S F    +  L+P+
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKY--TPPNQPHYNQDKTSEFVVKLLQFLVPL 120

BLAST of CaUC06G109380 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-20
Identity = 57/128 (44.53%), Postives = 77/128 (60.16%), Query Frame = 0

Query: 18  DSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS---------- 77
           + N KVY+ +EVAKHN + DCWLII G VY+V+ FL DHPGGD++LL ++          
Sbjct: 3   NDNKKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFED 62

Query: 78  VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP------TPSLFSFSPIHVLIPI 130
           VGHS  A+  M +Y +G ID ST+P + +YV   P QP      TP  F    +  L+P+
Sbjct: 63  VGHSTTARAMMDEYYVGDIDTSTIPARTKYV--PPKQPHYNQDKTPE-FIIKILQFLVPL 122

BLAST of CaUC06G109380 vs. ExPASy TrEMBL
Match: A0A1S3CAE5 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.1e-56
Identity = 115/131 (87.79%), Postives = 119/131 (90.84%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSKVYSFDEVAKHNHQKDCWLII+GYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNHQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSELAQEKMK YRIGKID+STLPKKQ YVE+TPYQPTPSLFSFSPIHVL+PILLLAL
Sbjct: 61  SVGHSELAQEKMKMYRIGKIDMSTLPKKQNYVESTPYQPTPSLFSFSPIHVLLPILLLAL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

BLAST of CaUC06G109380 vs. ExPASy TrEMBL
Match: A0A0A0KA20 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G107940 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 2.1e-52
Identity = 108/131 (82.44%), Postives = 116/131 (88.55%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSKVYSFDEVAKHN QKDCWLII+GYVYDVTSFLPDHPGGDELLLLA          
Sbjct: 1   MDSNSKVYSFDEVAKHNQQKDCWLIISGYVYDVTSFLPDHPGGDELLLLAVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSELA EKMK Y+IGKID+STLP+KQ+YVE+ PYQ TPSLFSFSPIH+L+PILLLAL
Sbjct: 61  SVGHSELAHEKMKMYQIGKIDMSTLPEKQKYVESAPYQRTPSLFSFSPIHLLLPILLLAL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPYFKLKA
Sbjct: 121 AFAFPYFKLKA 131

BLAST of CaUC06G109380 vs. ExPASy TrEMBL
Match: A0A6J1KHQ2 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.0e-47
Identity = 96/131 (73.28%), Postives = 111/131 (84.73%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSK+Y+F+EVAKHNH+KDCWLII+GYVYDVTS+LPDHPGG ELL+L           
Sbjct: 1   MDSNSKIYTFNEVAKHNHEKDCWLIISGYVYDVTSYLPDHPGGSELLVLTVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSE A+E MKKY+IGKID  TLP +Q+YVETTPY+PTPSL SFSP+HVLIP+LLL L
Sbjct: 61  SVGHSETAREMMKKYQIGKIDPLTLPTEQKYVETTPYKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

BLAST of CaUC06G109380 vs. ExPASy TrEMBL
Match: A0A6J1HJ10 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.4e-47
Identity = 96/131 (73.28%), Postives = 113/131 (86.26%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---------- 76
           MDSNSKVY+FDEVAKHNH+KDCWLII+G+VYDVTS+LPDHPGG ELL+LA          
Sbjct: 1   MDSNSKVYTFDEVAKHNHEKDCWLIISGFVYDVTSYLPDHPGGGELLVLAVEKDATFDFK 60

Query: 77  SVGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQPTPSLFSFSPIHVLIPILLLAL 136
           SVGHSE A+E+MKKY+IGKID  TLP +Q++VETTP++PTPSL SFSP+HVLIP+LLL L
Sbjct: 61  SVGHSESAKERMKKYQIGKIDPLTLPTEQKHVETTPHKPTPSLLSFSPLHVLIPLLLLVL 120

Query: 137 AFAFPYFKLKA 138
           AFAFPY K+KA
Sbjct: 121 AFAFPYLKIKA 131

BLAST of CaUC06G109380 vs. ExPASy TrEMBL
Match: A0A4Y1R0T5 (Cytochrome B5 isoform E (Fragment) OS=Prunus dulcis OX=3755 GN=Prudu_006899 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 3.0e-27
Identity = 69/139 (49.64%), Postives = 92/139 (66.19%), Query Frame = 0

Query: 11  REKLMEMDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLA---- 70
           +E    M S+S+VY FDEVAKHNH+KDCW+II+G VYDVT FL DHPGGDE+LLLA    
Sbjct: 19  KEAAAGMASDSQVYHFDEVAKHNHKKDCWIIISGKVYDVTPFLEDHPGGDEVLLLAVEKD 78

Query: 71  ------SVGHSELAQEKMKKYRIGKIDISTLPKKQEY---VETTPYQPTPSLFSFSPIHV 130
                  VGHS+ A+E+M+K+ +GK+D ST+P++  Y    +TTP     S F    +  
Sbjct: 79  ATDDFNDVGHSDSAKEQMEKFYVGKVDTSTIPEQPSYKLPAQTTPPSGQSSGFVVKLLQF 138

Query: 131 LIPILLLALAFAFPYFKLK 137
           L+P+L+L  AFA  Y+  K
Sbjct: 139 LLPLLILGAAFALQYYGKK 157

BLAST of CaUC06G109380 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-24
Identity = 64/136 (47.06%), Postives = 84/136 (61.76%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M S+ KV SF+EV+KHN  KDCWLII+G VYDVT F+ DHPGGDE+LL ++         
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP------TPSLFSFSPIHVLIP 136
            VGHS+ A++ M KY IG+ID S++P  + YV   P QP      TP  F    +  L+P
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYV--APQQPAYNQDKTPE-FIIKILQFLVP 120

BLAST of CaUC06G109380 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 103.2 bits (256), Expect = 1.7e-22
Identity = 54/126 (42.86%), Postives = 79/126 (62.70%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M   +K+++  EV++HN   DCW++ING VY+VT FL DHPGGD++LL ++         
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETTPYQP-----TPSLFSFSPIHVLIPI 128
            VGHSE A+E M++Y +G+ID +T+PKK +Y  T P QP       S F    +  L+P+
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKY--TPPKQPHYNQDKTSEFIIKLLQFLVPL 120

BLAST of CaUC06G109380 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 100.1 bits (248), Expect = 1.4e-21
Identity = 55/137 (40.15%), Postives = 81/137 (59.12%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M  + KV++  EV++H+  KDCW++I+G VYDVT FL DHPGGDE++L ++         
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQEYVETT-----PYQPTPSLFSFSPIHVLIPI 136
            VGHS  A+  + +Y +G ID +T+P K ++V  T       Q   S F    +  L+P+
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 137 LLLALAFAFPYF-KLKA 138
           L+L LAF   Y+ K KA
Sbjct: 121 LILGLAFGIRYYTKTKA 137

BLAST of CaUC06G109380 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 94.4 bits (233), Expect = 7.8e-20
Identity = 51/131 (38.93%), Postives = 79/131 (60.31%), Query Frame = 0

Query: 21  SKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS----------VGH 80
           + + SF +VAKH  + DCW++I+G VYD+++F+ +HPGGD +LL  +          V H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 81  SELAQEKMKKYRIGKIDISTLPKKQEYV-----ETTPYQPTPSLFSFSPIHVLIPILLLA 137
           S+ A+E MKKY IG +D ST+P  Q+Y+     E+T  + T        +  LIP+L+L 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

BLAST of CaUC06G109380 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 92.8 bits (229), Expect = 2.3e-19
Identity = 47/126 (37.30%), Postives = 78/126 (61.90%), Query Frame = 0

Query: 17  MDSNSKVYSFDEVAKHNHQKDCWLIINGYVYDVTSFLPDHPGGDELLLLAS--------- 76
           M + +K+YS +E A HN Q DCW++I+G VYDV+S++ +HPGGD++LL  +         
Sbjct: 1   MPTLTKLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFE 60

Query: 77  -VGHSELAQEKMKKYRIGKIDISTLPKKQE---YVETTPYQPTPSLFSFSPIHVLIPILL 130
             GHS+ A+E M+KY IG++D S+LP+  E   Y +  P      LF  +  + ++P+ +
Sbjct: 61  DAGHSKDARELMEKYFIGELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008459741.12.2e-5687.79PREDICTED: cytochrome b5 [Cucumis melo][more]
XP_038875836.14.1e-5582.71cytochrome b5-like [Benincasa hispida][more]
XP_004140502.14.3e-5282.44cytochrome b5 [Cucumis sativus] >KGN46545.1 hypothetical protein Csa_005454 [Cuc... [more]
XP_023000205.14.1e-4773.28cytochrome b5-like [Cucurbita maxima][more]
XP_022964486.19.2e-4773.28cytochrome b5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q423421.9e-2347.06Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2[more]
P409347.4e-2346.32Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1[more]
O488452.4e-2142.86Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1[more]
P490985.3e-2141.67Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P491001.5e-2044.53Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3CAE51.1e-5687.79cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103498784 PE=3 SV=1[more]
A0A0A0KA202.1e-5282.44Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
A0A6J1KHQ22.0e-4773.28cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111494495 PE=3 SV=1[more]
A0A6J1HJ104.4e-4773.28cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111464496 PE=3 SV=1[more]
A0A4Y1R0T53.0e-2749.64Cytochrome B5 isoform E (Fragment) OS=Prunus dulcis OX=3755 GN=Prudu_006899 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G53560.11.4e-2447.06cytochrome B5 isoform E [more]
AT2G32720.11.7e-2242.86cytochrome B5 isoform B [more]
AT5G48810.11.4e-2140.15cytochrome B5 isoform D [more]
AT2G46650.17.8e-2038.93cytochrome B5 isoform C [more]
AT1G26340.12.3e-1937.30cytochrome B5 isoform A [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 24..87
e-value: 7.1E-19
score: 78.7
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 27..66
e-value: 1.9E-14
score: 53.5
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 21..87
score: 19.794968
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 14..91
e-value: 8.0E-24
score: 85.4
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 18..92
NoneNo IPR availablePANTHERPTHR19359CYTOCHROME B5coord: 17..135
NoneNo IPR availablePANTHERPTHR19359:SF81SUBFAMILY NOT NAMEDcoord: 17..135
IPR018506Cytochrome b5, heme-binding sitePROSITEPS00191CYTOCHROME_B5_1coord: 52..59

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC06G109380.1CaUC06G109380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0020037 heme binding
molecular_function GO:0046872 metal ion binding