CaUC05G100940 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC05G100940
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionMajor facilitator superfamily protein
LocationCiama_Chr05: 31811308 .. 31813328 (+)
RNA-Seq ExpressionCaUC05G100940
SyntenyCaUC05G100940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTGACAGCCGGAACATTGTTCATGTACAGCATATATTCGAACGACATAAAATTTGTTTTGGGTTACGACCAAACGACATTGAATTTGCTGAGCTTTTCCAAGGATTTGGGCGCAAACGTGGGCATTTTGTCTGGGCTTATTAACGAGGTTACACCACCGTGGGTGGTTCTATCCATCGGTGCCGTTTTGAATTTCTTTGGTTACTTTATGATTTGGTTGGCTATTACGAAACGAATGTCTAGCCCCAAAGTTTGGCAAATGTGTCTTTATATTTGTATTGGAGCCAATTCTCAATCTTTTGCTAATACAAGATCTCTTGTTACTTGTGTCAAGAACTTCCCCGAGAGCCGTGGTGTTGTTCTTGGTAAGTATTTTAATTATAAGTTTAGTTCTCCAACTTTTAATTTTATTGTTTATTTGTTCTTTGAATATGAAATTGAAAAAAGAAAGTTTATGAATTAAGTGTCAATTTTGAATTCAATAAGTAGTCTATTAATGATTTTCCACAAATGGAAGAAAGACTAACGTTACGAGATTTTGCTTTTATTTCTTTAGAAGTGTCTAATAAATTTTTTAATTTTTAATTTTGTGTTTAATAGGTACCCGAACTTTTAGAGATGTTTAATGAGTCTTTAAACTCTTAACTATGCATCTAATAGTTAATTGAAGTCAAATAGTGTGTGTTTGAAATGTTCTTAATCTATTTAATATTTTCTTTTTTTTGAAAAATCACATACAGACTAGAGTGTGAAATTCAAAATCATATTTAAAATAAAATTAAAAATTCAAGAATTTATTAGACAATTTATAAAGGTAAAGAACTAAACTTATAATTTAATATTTAATTATTTAATTATTAGAGATGGTTTGTTGCCTAATTTCAAGCTTAGATTTTGTAAATGGTAGTTTTTGCTAGAGATTCAAACATTAGATTCCATATATGAAAAAAATAAAAATAAATAAATAACTACTAATTAAACTTAAACGTTAAAAAAAAATTAGGTATCTTGAAGGGATACGTCGGACTGAGCGGCGCCATAATCACACAACTCTTCCATGCATTTTACGGCAACGACACCAAATCCTTAATTCTCCTCATCGGATGGCTCCCGGCGGCAATTTCCTTCGCATTTTTACGAACAATTCGTATTATGAAAGTTATCCGACAGCCAAACGAGCTTAAAGTCTTCTACAATTTCCTTTACATTTCTCTTGGCCTCGCCGGATTCCTAATGCTAATGATCATCGTAGAGAGCAAAACAGAGTTCACTCAAAGCGAATACGGTGGCAGCGCCGCTGTCGTCCTTCTCCTCCTCTTCCTCCCACTCGCCGTCGTCATCATTGAAGAATACAATCTCTGGAAACTCAAAACCGTCCTCATTAAATCCCCAAATCCCTCTGTTCAAATCGTAACAGAGAAACTCCCAAAAACCGAGCAACTCCATAAGACAGAGCATCCAAAACAACAACAAAAAGAACCATCTTGTTGGACAACAATTTTCAACCCACCAGCGCGCGGCGAGGATTTCACAATTCTCCAAGCTCTCTTCAGTGTCGACATGCTGATTCTATTCCTCTCTGCAATTTGCGGCGTCGGCGGAACATTAACGGCAATAGACAATTTGGGTCAAATCGGAATGTCTTTGGGGTACCCAAAAAGAAGCATTAGCACATTTGTAACATTAGTCAGCATTTGGAATTACCTCGGCCGAGTTGCCTCTGGTTTCATCTCAGAAATAGTTTTGACAAAATACAAATTCCCTCGTCCTCTAATGCTCTCTCTGACCCTTCTTTTTTCCTGCATTGGCCATTTGATGATCGCATTTGATGTCCCAAATGGGCTTTATGTGGCTTCCGTCGTAATCGGATTTTGTTTTGGAGCACAGTGGCCATTGATTTTCGCAATAATTTCCGAACTTTTTGGATTGAAATATTACTCAACGCTTTTCAATTTTGGGTCAGTGGCGAGCCCAATTGGACTTTATGTTCTGAATGTGAAAGTGGCTGGAAATTTCTAG

mRNA sequence

ATGGTGACAGCCGGAACATTGTTCATGTACAGCATATATTCGAACGACATAAAATTTGTTTTGGGTTACGACCAAACGACATTGAATTTGCTGAGCTTTTCCAAGGATTTGGGCGCAAACGTGGGCATTTTGTCTGGGCTTATTAACGAGGTTACACCACCGTGGGTGGTTCTATCCATCGGTGCCGTTTTGAATTTCTTTGGTTACTTTATGATTTGGTTGGCTATTACGAAACGAATGTCTAGCCCCAAAGTTTGGCAAATGTGTCTTTATATTTGTATTGGAGCCAATTCTCAATCTTTTGCTAATACAAGATCTCTTGTTACTTGTGTCAAGAACTTCCCCGAGAGCCGTGGTGTTGTTCTTGGTATCTTGAAGGGATACGTCGGACTGAGCGGCGCCATAATCACACAACTCTTCCATGCATTTTACGGCAACGACACCAAATCCTTAATTCTCCTCATCGGATGGCTCCCGGCGGCAATTTCCTTCGCATTTTTACGAACAATTCGTATTATGAAAGTTATCCGACAGCCAAACGAGCTTAAAGTCTTCTACAATTTCCTTTACATTTCTCTTGGCCTCGCCGGATTCCTAATGCTAATGATCATCGTAGAGAGCAAAACAGAGTTCACTCAAAGCGAATACGGTGGCAGCGCCGCTGTCGTCCTTCTCCTCCTCTTCCTCCCACTCGCCGTCGTCATCATTGAAGAATACAATCTCTGGAAACTCAAAACCGTCCTCATTAAATCCCCAAATCCCTCTGTTCAAATCGTAACAGAGAAACTCCCAAAAACCGAGCAACTCCATAAGACAGAGCATCCAAAACAACAACAAAAAGAACCATCTTGTTGGACAACAATTTTCAACCCACCAGCGCGCGGCGAGGATTTCACAATTCTCCAAGCTCTCTTCAGTGTCGACATGCTGATTCTATTCCTCTCTGCAATTTGCGGCGTCGGCGGAACATTAACGGCAATAGACAATTTGGGTCAAATCGGAATGTCTTTGGGGTACCCAAAAAGAAGCATTAGCACATTTGTAACATTAGTCAGCATTTGGAATTACCTCGGCCGAGTTGCCTCTGGTTTCATCTCAGAAATAGTTTTGACAAAATACAAATTCCCTCGTCCTCTAATGCTCTCTCTGACCCTTCTTTTTTCCTGCATTGGCCATTTGATGATCGCATTTGATGTCCCAAATGGGCTTTATGTGGCTTCCGTCGTAATCGGATTTTGTTTTGGAGCACAGTGGCCATTGATTTTCGCAATAATTTCCGAACTTTTTGGATTGAAATATTACTCAACGCTTTTCAATTTTGGGTCAGTGGCGAGCCCAATTGGACTTTATGTTCTGAATGTGAAAGTGGCTGGAAATTTCTAG

Coding sequence (CDS)

ATGGTGACAGCCGGAACATTGTTCATGTACAGCATATATTCGAACGACATAAAATTTGTTTTGGGTTACGACCAAACGACATTGAATTTGCTGAGCTTTTCCAAGGATTTGGGCGCAAACGTGGGCATTTTGTCTGGGCTTATTAACGAGGTTACACCACCGTGGGTGGTTCTATCCATCGGTGCCGTTTTGAATTTCTTTGGTTACTTTATGATTTGGTTGGCTATTACGAAACGAATGTCTAGCCCCAAAGTTTGGCAAATGTGTCTTTATATTTGTATTGGAGCCAATTCTCAATCTTTTGCTAATACAAGATCTCTTGTTACTTGTGTCAAGAACTTCCCCGAGAGCCGTGGTGTTGTTCTTGGTATCTTGAAGGGATACGTCGGACTGAGCGGCGCCATAATCACACAACTCTTCCATGCATTTTACGGCAACGACACCAAATCCTTAATTCTCCTCATCGGATGGCTCCCGGCGGCAATTTCCTTCGCATTTTTACGAACAATTCGTATTATGAAAGTTATCCGACAGCCAAACGAGCTTAAAGTCTTCTACAATTTCCTTTACATTTCTCTTGGCCTCGCCGGATTCCTAATGCTAATGATCATCGTAGAGAGCAAAACAGAGTTCACTCAAAGCGAATACGGTGGCAGCGCCGCTGTCGTCCTTCTCCTCCTCTTCCTCCCACTCGCCGTCGTCATCATTGAAGAATACAATCTCTGGAAACTCAAAACCGTCCTCATTAAATCCCCAAATCCCTCTGTTCAAATCGTAACAGAGAAACTCCCAAAAACCGAGCAACTCCATAAGACAGAGCATCCAAAACAACAACAAAAAGAACCATCTTGTTGGACAACAATTTTCAACCCACCAGCGCGCGGCGAGGATTTCACAATTCTCCAAGCTCTCTTCAGTGTCGACATGCTGATTCTATTCCTCTCTGCAATTTGCGGCGTCGGCGGAACATTAACGGCAATAGACAATTTGGGTCAAATCGGAATGTCTTTGGGGTACCCAAAAAGAAGCATTAGCACATTTGTAACATTAGTCAGCATTTGGAATTACCTCGGCCGAGTTGCCTCTGGTTTCATCTCAGAAATAGTTTTGACAAAATACAAATTCCCTCGTCCTCTAATGCTCTCTCTGACCCTTCTTTTTTCCTGCATTGGCCATTTGATGATCGCATTTGATGTCCCAAATGGGCTTTATGTGGCTTCCGTCGTAATCGGATTTTGTTTTGGAGCACAGTGGCCATTGATTTTCGCAATAATTTCCGAACTTTTTGGATTGAAATATTACTCAACGCTTTTCAATTTTGGGTCAGTGGCGAGCCCAATTGGACTTTATGTTCTGAATGTGAAAGTGGCTGGAAATTTCTAG

Protein sequence

MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSIGAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYNLWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF
Homology
BLAST of CaUC05G100940 vs. NCBI nr
Match: XP_008446819.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK09246.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 818.9 bits (2114), Expect = 2.2e-233
Identity = 417/461 (90.46%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+ +YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAVLNFFGYFMIWLA+T+R+S+PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFA LRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESK EF Q+EYGGSAAVVLLLLFLPL+VVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYN 274

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQ-QQKEPSCWTTIFNPPARGEDFT 300
           LWKLKT L+KSPNPSVQIVTEKLP      KTEHPKQ +QKEPSCWTTIFNPP RGEDFT
Sbjct: 275 LWKLKTALVKSPNPSVQIVTEKLP------KTEHPKQLEQKEPSCWTTIFNPPQRGEDFT 334

Query: 301 ILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR 360
           ILQALFS DMLILF++AICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR
Sbjct: 335 ILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR 394

Query: 361 VASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQWP 420
           VASGFISEIVLTKYKFPRPL+LSLTLL SC+GHL+IAFDVPNGLYVAS+VIGFCFGAQWP
Sbjct: 395 VASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWP 454

Query: 421 LIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           LIFAIISELFGLKYYSTL+NFGSVASPIGLY+LNVKVAGNF
Sbjct: 455 LIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 489

BLAST of CaUC05G100940 vs. NCBI nr
Match: XP_038893353.1 (LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 816.6 bits (2108), Expect = 1.1e-232
Identity = 409/460 (88.91%), Postives = 439/460 (95.43%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+ +YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFGLYSDDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GA+LNFFGYFMIWLA+T+R+S+PKVWQMC+YICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAILNFFGYFMIWLAVTRRISAPKVWQMCVYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRI+KVIR PN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIIKVIRHPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISLGLAGFLMLMII+ESKTEFTQ+EYGGSAAVVL+LLFLPLAVVIIEEYN
Sbjct: 215 ELKVFYNFLYISLGLAGFLMLMIIIESKTEFTQNEYGGSAAVVLVLLFLPLAVVIIEEYN 274

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARGEDFTI 300
           LWKLKT  IKS NP VQIVTE+LPKTE  H  + PKQ+QKEPSCWTT+F+PP RGEDFTI
Sbjct: 275 LWKLKTAFIKSSNPCVQIVTEQLPKTE--HPKQTPKQEQKEPSCWTTVFSPPERGEDFTI 334

Query: 301 LQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRV 360
           LQALFS+DMLILFLSA CGVGGTLTAIDNLGQIGMSLGYPKRSISTFV+LVSIWNYLGR+
Sbjct: 335 LQALFSIDMLILFLSATCGVGGTLTAIDNLGQIGMSLGYPKRSISTFVSLVSIWNYLGRI 394

Query: 361 ASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQWPL 420
           ASGF+SEIV+TKYKFPRPL+LSLTLL SC+GHLMIAFDVPNGLYVASVVIGFCFGAQWP+
Sbjct: 395 ASGFVSEIVVTKYKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASVVIGFCFGAQWPV 454

Query: 421 IFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           IFAIISELFGLKYYSTL+NFGSVASPIGLY+LNVKVAGNF
Sbjct: 455 IFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 492

BLAST of CaUC05G100940 vs. NCBI nr
Match: XP_004142499.3 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothetical protein Csa_008497 [Cucumis sativus])

HSP 1 Score: 815.1 bits (2104), Expect = 3.1e-232
Identity = 413/460 (89.78%), Postives = 438/460 (95.22%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+ +YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAVLNFFGYFMIWLA+T+R+S+PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVLNFFGYFMIWLAVTRRISAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYG+DTKSLILLIGWLPAAISFA LRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGDDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESK +F Q+EYGGSAAVVLLLL LPLAVVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKKQFNQNEYGGSAAVVLLLLILPLAVVIIEEYN 274

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARGEDFTI 300
           LWKLKT +IKSPNPSVQIVTEKLP      KTEHPKQ++KEPSCWTTIF+PP RGEDFTI
Sbjct: 275 LWKLKTAVIKSPNPSVQIVTEKLP------KTEHPKQERKEPSCWTTIFSPPQRGEDFTI 334

Query: 301 LQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGRV 360
           LQALFSVDMLILF++AICGVGGTLTAIDNLGQIG++LGYPKRSISTFV+LVSIWNYLGRV
Sbjct: 335 LQALFSVDMLILFIAAICGVGGTLTAIDNLGQIGLALGYPKRSISTFVSLVSIWNYLGRV 394

Query: 361 ASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQWPL 420
           ASGFISEIVLTKYKFPRPL+LSLTLL SC+GHLMIAFDVPNGLYVAS+VIGFCFGAQWPL
Sbjct: 395 ASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLMIAFDVPNGLYVASIVIGFCFGAQWPL 454

Query: 421 IFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           IFAIISELFGLKYYSTL+NFGSVASPIGLYVLNVKVAGNF
Sbjct: 455 IFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNF 488

BLAST of CaUC05G100940 vs. NCBI nr
Match: XP_022956604.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 803.9 bits (2075), Expect = 7.2e-229
Identity = 413/465 (88.82%), Postives = 436/465 (93.76%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+S+YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFSLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAV+NFFGYFMIWLA+T+++++PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVMNFFGYFMIWLAVTRKIAAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYG DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESKTEF+QS YGGSAAVVLLLLFLPLAVVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYN 274

Query: 241 LWKLK----TVLIKSPNPSVQIVTEKLPKTEQLHKTEHPK-QQQKEPSCWTTIFNPPARG 300
           LW LK    T  I+SPN  VQIVTEKLPKTE  H+   PK + QKEPSCWTTIFNPP RG
Sbjct: 275 LWNLKTAAATAAIRSPNHPVQIVTEKLPKTE--HQKHIPKTEHQKEPSCWTTIFNPPERG 334

Query: 301 EDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWN 360
           EDFTILQALFSVDMLILFL+AICGVGGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWN
Sbjct: 335 EDFTILQALFSVDMLILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSIWN 394

Query: 361 YLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFG 420
           YLGRV SGF+SEIVLTKYKFPRPLMLS+TLL SC+GHLMIAFDVPNGLYVAS+VIGFCFG
Sbjct: 395 YLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFG 454

Query: 421 AQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           AQWPLIFAIISELFGLKYYSTL+NFGSVASPIGLYVLNVKVAGNF
Sbjct: 455 AQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNF 497

BLAST of CaUC05G100940 vs. NCBI nr
Match: XP_023535761.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.4 bits (2071), Expect = 2.1e-228
Identity = 414/467 (88.65%), Postives = 435/467 (93.15%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+S+YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFSLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAV+NFFGYFMIWLA+T+++S+PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVMNFFGYFMIWLAVTQKISAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYG DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESKTEF+QS YGGSAAVVLLLLFLPLAVVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYN 274

Query: 241 LWKLK----TVLIKSPNPSVQIVTEKLPKTE---QLHKTEHPKQQQKEPSCWTTIFNPPA 300
           LW LK    T  I+SPN  VQIVTEK+PKTE    + KTEH KQ    PSCWTTIFNPP 
Sbjct: 275 LWNLKTAATTAAIRSPNHPVQIVTEKVPKTEHQKHIPKTEHKKQ----PSCWTTIFNPPE 334

Query: 301 RGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSI 360
           RGEDFTILQALFSVDMLILFL+AICGVGGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSI
Sbjct: 335 RGEDFTILQALFSVDMLILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSI 394

Query: 361 WNYLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFC 420
           WNYLGRV SGF+SEIVLTKYKFPRPLMLS+TLL SC+GHLMIAFDVPNGLYVASVVIGFC
Sbjct: 395 WNYLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASVVIGFC 454

Query: 421 FGAQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           FGAQWPLIFAIISELFGLKYYSTL+NFGSVASPIGLYVLNVKVAGNF
Sbjct: 455 FGAQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNF 497

BLAST of CaUC05G100940 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 3.2e-22
Identity = 115/460 (25.00%), Postives = 204/460 (44.35%), Query Frame = 0

Query: 3   TAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSIGA 62
           + GT F +S YS+ +K VLG  Q  LN L+ + DLG   G  SG+     P  VVL   A
Sbjct: 56  STGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIALGYFPLSVVLFAAA 115

Query: 63  VLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGAN-SQSFANTRSLVTCVKNFPESRGVV 122
            + F GY + WL IT  ++ P  + +    C+ A  S  + NT   + C+++FP +R + 
Sbjct: 116 AMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLSICWFNTACFILCIRHFPNNRALA 175

Query: 123 LGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVI----- 182
           L +   + G+S A+ +  F+A   + +   +LL   +P  +SFA L  +     +     
Sbjct: 176 LSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYPVLTKPSLDTTPD 235

Query: 183 ----RQPNELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLA 242
               R  + +    N L +   +  F +L  +  S T   +  + G+  +++  L  PL 
Sbjct: 236 YDSRRHDSHVFTILNVLAV---ITSFHLL--LSSSSTSSARLNFIGAVVLLVFPLCAPL- 295

Query: 243 VVIIEEYNLWKLKTVLIKSPNPSVQIVTEKL--PKTEQLHKTEHPKQQQKEPSCWTTIFN 302
           +V   +Y L  +   L    +  V +  ++L   KT    KT +      +        N
Sbjct: 296 LVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKEG------N 355

Query: 303 PPARGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTL 362
               G++ +    +  ++  + +++  CG    L   +NLGQI  SLG   ++ +T VT+
Sbjct: 356 TVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---QNSTTLVTI 415

Query: 363 VSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAF--DVPNGLYVASV 422
            S +++ GR+ S    + +  +++  R    ++ LL + I   ++A        L  A+ 
Sbjct: 416 YSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQTATA 475

Query: 423 VIGFCFGAQWPLIFAIISELFGLKYYSTLFNFGSVASPIG 449
           +IG   G  +    +I S+LFG        N      PIG
Sbjct: 476 LIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIG 497

BLAST of CaUC05G100940 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 2.3e-04
Identity = 100/466 (21.46%), Postives = 192/466 (41.20%), Query Frame = 0

Query: 4   AGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGAN-VGILSGLINEVTPPWVVLSIGA 63
           AGT +++S Y+  +        +  + LSFS  +G++ +GIL+G++ + +P    L IG+
Sbjct: 32  AGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGSSLMGILAGIVVDRSPKLSCL-IGS 91

Query: 64  VLNFFGYFMIWLAITKRMSSPKVWQMCL-YICIGANSQSFANTRSLVTCVKNFPESRGVV 123
           +  F  Y ++ L      SS  +  + L  I  G+ S  +A+ +   T   NFP+ RG  
Sbjct: 92  MCVFIAYLILNLCYKHEWSSTFLISLSLVLIGYGSVSGFYASVKCANT---NFPQHRGTA 151

Query: 124 LGILKGYVGLSGAIITQLFHAFYGNDTKSL----------ILLIGWLPAAI------SFA 183
                   GLSG + + L    +G + + +          ++L+G+    I        A
Sbjct: 152 GAFPVSLYGLSGMVFSYLCSKLFGENIEHVFIFLMVACGCMILVGYFSLDIFSNAEGDDA 211

Query: 184 FLRTIRIMKVIRQPNELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLL 243
            ++   + K     + +   Y      +G          V S +  T   Y  S      
Sbjct: 212 SIKEWELQKSRETDDNIVPLYENSNDYIGSP--------VRSSSPATYETYALSDNFQET 271

Query: 244 LLFLPLAVVIIEEYNLWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCW 303
             F  L     E+  L     +   SP+       E   K       E+  Q+      +
Sbjct: 272 SEFFAL-----EDRQLSNRPLLSPSSPHTKYDFEDENTSKNT---VGENSAQKSMRLHVF 331

Query: 304 TTIFNPPARGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSIS 363
            ++ +    G  + +L  L  V ++ ++      VG  + A  +   +       ++  S
Sbjct: 332 QSLKSSTFIGY-YIVLGILQGVGLMYIY-----SVGFMVQAQVSTPPLNQLPINAEKIQS 391

Query: 364 TFVTLVSIWNYLGRVASGFISEIVLTKYKFPR--PLMLSLTLLF---SCIGHLMIAFDVP 423
             VTL+S+ ++ GR++SG IS+ ++ K+K  R   ++++  L+F   + I H   + + P
Sbjct: 392 LQVTLLSLLSFCGRLSSGPISDFLVKKFKAQRLWNIVIASLLVFLASNKISHDFSSIEDP 451

Query: 424 N--------GLYVASVVIGFCFGAQWPLIFAIISELFGLKYYSTLF 439
           +         + V S + G+ FG  +    +I+++ FG   YSTL+
Sbjct: 452 SLRASKSFKNISVCSAIFGYSFGVLFGTFPSIVADRFGTNGYSTLW 471

BLAST of CaUC05G100940 vs. ExPASy TrEMBL
Match: A0A5D3CFW3 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002400 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.0e-233
Identity = 417/461 (90.46%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+ +YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAVLNFFGYFMIWLA+T+R+S+PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFA LRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESK EF Q+EYGGSAAVVLLLLFLPL+VVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYN 274

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQ-QQKEPSCWTTIFNPPARGEDFT 300
           LWKLKT L+KSPNPSVQIVTEKLP      KTEHPKQ +QKEPSCWTTIFNPP RGEDFT
Sbjct: 275 LWKLKTALVKSPNPSVQIVTEKLP------KTEHPKQLEQKEPSCWTTIFNPPQRGEDFT 334

Query: 301 ILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR 360
           ILQALFS DMLILF++AICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR
Sbjct: 335 ILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR 394

Query: 361 VASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQWP 420
           VASGFISEIVLTKYKFPRPL+LSLTLL SC+GHL+IAFDVPNGLYVAS+VIGFCFGAQWP
Sbjct: 395 VASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWP 454

Query: 421 LIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           LIFAIISELFGLKYYSTL+NFGSVASPIGLY+LNVKVAGNF
Sbjct: 455 LIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 489

BLAST of CaUC05G100940 vs. ExPASy TrEMBL
Match: A0A1S3BG09 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 PE=4 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.0e-233
Identity = 417/461 (90.46%), Postives = 438/461 (95.01%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+ +YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFGLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAVLNFFGYFMIWLA+T+R+S+PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVLNFFGYFMIWLAVTRRISTPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFA LRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFASLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESK EF Q+EYGGSAAVVLLLLFLPL+VVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKKEFNQNEYGGSAAVVLLLLFLPLSVVIIEEYN 274

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQ-QQKEPSCWTTIFNPPARGEDFT 300
           LWKLKT L+KSPNPSVQIVTEKLP      KTEHPKQ +QKEPSCWTTIFNPP RGEDFT
Sbjct: 275 LWKLKTALVKSPNPSVQIVTEKLP------KTEHPKQLEQKEPSCWTTIFNPPQRGEDFT 334

Query: 301 ILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR 360
           ILQALFS DMLILF++AICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR
Sbjct: 335 ILQALFSFDMLILFIAAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLGR 394

Query: 361 VASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQWP 420
           VASGFISEIVLTKYKFPRPL+LSLTLL SC+GHL+IAFDVPNGLYVAS+VIGFCFGAQWP
Sbjct: 395 VASGFISEIVLTKYKFPRPLILSLTLLLSCVGHLIIAFDVPNGLYVASIVIGFCFGAQWP 454

Query: 421 LIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           LIFAIISELFGLKYYSTL+NFGSVASPIGLY+LNVKVAGNF
Sbjct: 455 LIFAIISELFGLKYYSTLYNFGSVASPIGLYILNVKVAGNF 489

BLAST of CaUC05G100940 vs. ExPASy TrEMBL
Match: A0A6J1GZI8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111458291 PE=4 SV=1)

HSP 1 Score: 803.9 bits (2075), Expect = 3.5e-229
Identity = 413/465 (88.82%), Postives = 436/465 (93.76%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+S+YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFSLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAV+NFFGYFMIWLA+T+++++PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVMNFFGYFMIWLAVTRKIAAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYG DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMIIVESKTEF+QS YGGSAAVVLLLLFLPLAVVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIVESKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYN 274

Query: 241 LWKLK----TVLIKSPNPSVQIVTEKLPKTEQLHKTEHPK-QQQKEPSCWTTIFNPPARG 300
           LW LK    T  I+SPN  VQIVTEKLPKTE  H+   PK + QKEPSCWTTIFNPP RG
Sbjct: 275 LWNLKTAAATAAIRSPNHPVQIVTEKLPKTE--HQKHIPKTEHQKEPSCWTTIFNPPERG 334

Query: 301 EDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWN 360
           EDFTILQALFSVDMLILFL+AICGVGGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWN
Sbjct: 335 EDFTILQALFSVDMLILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSIWN 394

Query: 361 YLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFG 420
           YLGRV SGF+SEIVLTKYKFPRPLMLS+TLL SC+GHLMIAFDVPNGLYVAS+VIGFCFG
Sbjct: 395 YLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFG 454

Query: 421 AQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           AQWPLIFAIISELFGLKYYSTL+NFGSVASPIGLYVLNVKVAGNF
Sbjct: 455 AQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNF 497

BLAST of CaUC05G100940 vs. ExPASy TrEMBL
Match: A0A6J1K186 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489721 PE=4 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.8e-226
Identity = 407/465 (87.53%), Postives = 429/465 (92.26%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+S+YS+DIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 35  MAAAGATYMFSLYSSDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 94

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAV+NFFGYFMIWLA+T ++S+PKVWQMCLYICIGANSQSFANT SLVTCVKNFPESRGV
Sbjct: 95  GAVMNFFGYFMIWLAVTGKISAPKVWQMCLYICIGANSQSFANTGSLVTCVKNFPESRGV 154

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYG DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN
Sbjct: 155 VLGILKGYVGLSGAIITQLFHAFYGGDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 214

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMII+ESKTEF+QS YGGSAAVVLLLLFLPLAVVIIEEYN
Sbjct: 215 ELKVFYNFLYISLALAGFLMLMIIIESKTEFSQSGYGGSAAVVLLLLFLPLAVVIIEEYN 274

Query: 241 LWKLKT-----VLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARG 300
           LW LKT       I+SPN  VQIVTEK+PKTE           QK+PSCWTTIFNPP RG
Sbjct: 275 LWNLKTAAATAAAIRSPNHPVQIVTEKVPKTE----------HQKQPSCWTTIFNPPERG 334

Query: 301 EDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWN 360
           EDFTILQALFSVDM ILFL+AICGVGGTLTAIDNLGQIGMSLGYPK+SISTFVTLVSIWN
Sbjct: 335 EDFTILQALFSVDMFILFLAAICGVGGTLTAIDNLGQIGMSLGYPKQSISTFVTLVSIWN 394

Query: 361 YLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFG 420
           YLGRV SGF+SEIVLTKYKFPRPLMLS+TLL SC+GHLMIAFDVPNGLYVAS+VIGFCFG
Sbjct: 395 YLGRVTSGFVSEIVLTKYKFPRPLMLSMTLLVSCVGHLMIAFDVPNGLYVASIVIGFCFG 454

Query: 421 AQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGNF 461
           AQWPLIFAIISELFGLKYYSTL+NFGSVASPIGLYVLNVKVAGNF
Sbjct: 455 AQWPLIFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGNF 489

BLAST of CaUC05G100940 vs. ExPASy TrEMBL
Match: A0A6J1FZF2 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 5.4e-222
Identity = 401/461 (86.98%), Postives = 429/461 (93.06%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +M+S+YSNDIK VLGYDQTTLNLLSF KDLGANVG+LSGLINEVTPPWVVLSI
Sbjct: 40  MAAAGATYMFSLYSNDIKSVLGYDQTTLNLLSFFKDLGANVGVLSGLINEVTPPWVVLSI 99

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GAVLNFFGYFMIWLA+TKR+S+PKVWQMCLYIC+GANSQSF NT SLVTCVKNFPESRGV
Sbjct: 100 GAVLNFFGYFMIWLAVTKRISTPKVWQMCLYICVGANSQSFVNTGSLVTCVKNFPESRGV 159

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQLFHAFYG+DTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN
Sbjct: 160 VLGILKGYVGLSGAIITQLFHAFYGSDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 219

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISL LAGFLMLMII+ESK  F+Q+E+GGSAAVVLLLLFLPLAVVI+EEYN
Sbjct: 220 ELKVFYNFLYISLALAGFLMLMIIIESKLIFSQAEFGGSAAVVLLLLFLPLAVVIVEEYN 279

Query: 241 LWKLKTVLIKSPNPSVQIVTEK--LPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARGEDF 300
           L KLK+  I  PNP +QI+TEK   PKTE   +T    +QQK+PSCWTTIF+PP RGEDF
Sbjct: 280 LSKLKSAAINPPNP-LQILTEKPNPPKTEPQKQT----RQQKQPSCWTTIFSPPERGEDF 339

Query: 301 TILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLG 360
           TILQALFS+DM ILF SAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLG
Sbjct: 340 TILQALFSLDMAILFFSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYLG 399

Query: 361 RVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQW 420
           RVASGF+SEIVLTKYKFPRPLMLSLTLL SC+GHL+IAF VPNGLYVAS+VIGFCFGAQW
Sbjct: 400 RVASGFVSEIVLTKYKFPRPLMLSLTLLLSCVGHLIIAFAVPNGLYVASIVIGFCFGAQW 459

Query: 421 PLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGN 460
           PL+FAIISELFGLKYYSTL+NFGSVASPIGLYVLNVKVAGN
Sbjct: 460 PLLFAIISELFGLKYYSTLYNFGSVASPIGLYVLNVKVAGN 495

BLAST of CaUC05G100940 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 694.1 bits (1790), Expect = 7.5e-200
Identity = 350/465 (75.27%), Postives = 398/465 (85.59%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M TAG  +M+ IYS DIK  LGYDQTTLNLLSF KDLGANVG+L+GL+NEVTPPW +L I
Sbjct: 32  MSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVLAGLLNEVTPPWFILLI 91

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           GA+LNFFGYFMIWLA+T+R+S P+VW MCLYIC+GANSQSFANT SLVTCVKNFPESRGV
Sbjct: 92  GAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANTGSLVTCVKNFPESRGV 151

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           VLGILKGYVGLSGAIITQL+ AFYG DTK LIL+IGWLPA +SFAFLRTIRIMKV RQ N
Sbjct: 152 VLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSFAFLRTIRIMKVKRQTN 211

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           ELKVFYNFLYISLGLA FLM++II+   + FTQSE+GGSAAVV++LL LP+ VVI+EE  
Sbjct: 212 ELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVIVLLLLPIIVVILEEKK 271

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKE-------PSCWTTIFNPPA 300
           LWK K V +  P P + +VTEK PK +     +   ++ KE       PSCWTT+FNPP 
Sbjct: 272 LWKEKQVALNDPAP-INVVTEK-PKLDSSEFKDDDGEESKEVVEKVKTPSCWTTVFNPPE 331

Query: 301 RGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSI 360
           RG+D+TILQALFSVDMLILFL+ ICGVGGTLTAIDNLGQIG SLGYPKRS+STFV+LVSI
Sbjct: 332 RGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLGYPKRSVSTFVSLVSI 391

Query: 361 WNYLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFC 420
           WNY GRV SG +SEI L KYKFPRPLML++ LL SC GHL+IAF+VP GLYVASV+IGFC
Sbjct: 392 WNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFC 451

Query: 421 FGAQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAG 459
           FGAQWPL+FAIISE+FGLKYYSTL+NFGSVASPIG Y+LNV+VAG
Sbjct: 452 FGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 494

BLAST of CaUC05G100940 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 570.9 bits (1470), Expect = 9.6e-163
Identity = 285/461 (61.82%), Postives = 358/461 (77.66%), Query Frame = 0

Query: 1   MVTAGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSI 60
           M  AG  +++  YS DIK  LGYDQTTLNLL F KDLGANVG+LSGLI EVTP W VL+I
Sbjct: 28  MACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLIAEVTPTWFVLTI 87

Query: 61  GAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGV 120
           G+ +NF GYFMIWL +T +++ PKVWQMCLYICIGANSQ+FANT +LVTCVKNFPESRGV
Sbjct: 88  GSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALVTCVKNFPESRGV 147

Query: 121 VLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPN 180
           +LG+LKGYVGLSGAI TQL+ A YG+D+KSLILLI WLPAA+S  F+  IR  KV+RQ N
Sbjct: 148 MLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVYLIREKKVVRQRN 207

Query: 181 ELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAVVIIEEYN 240
           EL VFY FLYIS+ LA FLM M I E +  F+++ Y  SA +   LLF+PL V + +E  
Sbjct: 208 ELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLFVPLTVSVKQELE 267

Query: 241 LWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPK---QQQKEPSCWTTIFNPPARGED 300
           +W +  + I+ P+   ++  EK  K   L + +  K   ++++  SC++T+F+PP RGED
Sbjct: 268 VWNMMKLPIEEPS---EVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTVFSPPPRGED 327

Query: 301 FTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYL 360
           +TILQAL S DM+ILF++  CG+G +LTA+DNLGQIG SLGYP  ++S+FV+LVSIWNY 
Sbjct: 328 YTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFVSLVSIWNYF 387

Query: 361 GRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQ 420
           GRV SGF+SE +L KYK PRPLM++L LL SC GHL+IAF VP  +Y+AS+++GF FGAQ
Sbjct: 388 GRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASILMGFSFGAQ 447

Query: 421 WPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAG 459
            PL+FAIISELFGLKYYSTLFN G +ASP+G Y+LNV+V G
Sbjct: 448 LPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTG 485

BLAST of CaUC05G100940 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 8.9e-76
Identity = 172/462 (37.23%), Postives = 272/462 (58.87%), Query Frame = 0

Query: 5   GTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSIGAVL 64
           G LF  S+ S  IK  LGY+Q  + LL  +K+LG  +G +SG ++EV+P WVVL +GA  
Sbjct: 33  GYLFGGSM-SPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGATQ 92

Query: 65  NFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGVVLGI 124
           N FGY ++WL +T ++ +  +W + + I +G N +++ NT SLV+C+ NFPESRG V+GI
Sbjct: 93  NLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVGI 152

Query: 125 LKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKV 184
           LKG+ GLSGAI+TQ++  F  +   S+IL++   P  +  A L  +R ++   + N    
Sbjct: 153 LKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNLRSD 212

Query: 185 FYNFLYI---SLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAV----VIIE 244
              FL I    + LA +L+ +++++S  + TQ+    S A++++ + +P+ V    V I 
Sbjct: 213 DLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSSVFIS 272

Query: 245 EYNLWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARGED 304
             N+  +K      P      V +   +T  + +++ P ++++ P            GED
Sbjct: 273 GNNVTSVK------PEEGTSNVDQHEART-LIERSDRPPEKKRAPCI----------GED 332

Query: 305 FTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYL 364
           FT+LQAL   D  ++F+S + GVG  +T IDNLGQI  SLGY    I  FV+L+SI N+L
Sbjct: 333 FTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKI--FVSLISISNFL 392

Query: 365 GRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQ 424
           GRVA G+ SE+++ K   PR L +S+      +G +  A D P  +YV ++VIG  +GA 
Sbjct: 393 GRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAH 452

Query: 425 WPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGN 460
           W +  A +S++FGLK + +L+NF   A PIG +V +  +A N
Sbjct: 453 WAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASN 474

BLAST of CaUC05G100940 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 8.9e-76
Identity = 172/462 (37.23%), Postives = 272/462 (58.87%), Query Frame = 0

Query: 5   GTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGLINEVTPPWVVLSIGAVL 64
           G LF  S+ S  IK  LGY+Q  + LL  +K+LG  +G +SG ++EV+P WVVL +GA  
Sbjct: 33  GYLFGGSM-SPAIKTSLGYNQKQIALLGVAKNLGDAIGFVSGALSEVSPSWVVLLVGATQ 92

Query: 65  NFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSLVTCVKNFPESRGVVLGI 124
           N FGY ++WL +T ++ +  +W + + I +G N +++ NT SLV+C+ NFPESRG V+GI
Sbjct: 93  NLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETYYNTASLVSCIHNFPESRGPVVGI 152

Query: 125 LKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFLRTIRIMKVIRQPNELKV 184
           LKG+ GLSGAI+TQ++  F  +   S+IL++   P  +  A L  +R ++   + N    
Sbjct: 153 LKGFSGLSGAILTQVYLMFNPSHDSSVILMVALGPPVVVLALLFIVRPVERSCRTNLRSD 212

Query: 185 FYNFLYI---SLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLLFLPLAV----VIIE 244
              FL I    + LA +L+ +++++S  + TQ+    S A++++ + +P+ V    V I 
Sbjct: 213 DLRFLAIYGFCVVLAVYLLGLLVLQSVFDMTQTIITTSGAILVIFMVVPVLVPFSSVFIS 272

Query: 245 EYNLWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTTIFNPPARGED 304
             N+  +K      P      V +   +T  + +++ P ++++ P            GED
Sbjct: 273 GNNVTSVK------PEEGTSNVDQHEART-LIERSDRPPEKKRAPCI----------GED 332

Query: 305 FTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTFVTLVSIWNYL 364
           FT+LQAL   D  ++F+S + GVG  +T IDNLGQI  SLGY    I  FV+L+SI N+L
Sbjct: 333 FTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYSNTKI--FVSLISISNFL 392

Query: 365 GRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVASVVIGFCFGAQ 424
           GRVA G+ SE+++ K   PR L +S+      +G +  A D P  +YV ++VIG  +GA 
Sbjct: 393 GRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWPGKIYVVTIVIGMGYGAH 452

Query: 425 WPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAGN 460
           W +  A +S++FGLK + +L+NF   A PIG +V +  +A N
Sbjct: 453 WAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASN 474

BLAST of CaUC05G100940 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 275.0 bits (702), Expect = 1.1e-73
Identity = 169/471 (35.88%), Postives = 264/471 (56.05%), Query Frame = 0

Query: 4   AGTLFMYSIYSNDIKFVLGYDQTTLNLLSFSKDLGANVGILSGL---------------- 63
           +G  + + IYS  +K    YDQ+TL+ +S  KD+GAN G+ SGL                
Sbjct: 21  SGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVFSGLLYTYATSNRLRGRGGG 80

Query: 64  INEVTPPWVVLSIGAVLNFFGYFMIWLAITKRMSSPKVWQMCLYICIGANSQSFANTRSL 123
           I     PWVVL++GA+  F GYF+IW ++T  +  P V  MCL++ + A SQ+F NT ++
Sbjct: 81  IGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLMCLFMFLAAQSQTFFNTANV 140

Query: 124 VTCVKNFPESRGVVLGILKGYVGLSGAIITQLFHAFYGNDTKSLILLIGWLPAAISFAFL 183
           V+ V+NF +  G  +GI+KG++GLSGAI+ QL+      D  S ILL+   P  +S   +
Sbjct: 141 VSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDPASFILLLAVTPTVLSLLVM 200

Query: 184 RTIRIMKVIRQPNELKVFYNFLYISLGLAGFLMLMIIVESKTEFTQSEYGGSAAVVLLLL 243
             +RI +     ++ K       +SL +A +LM++II+  K  F  S +     +V LL+
Sbjct: 201 PLVRIYET-SVADDKKHLNGLSAVSLIIAAYLMIIIIL--KNTFGLSSWANIVTLVCLLV 260

Query: 244 FLPLAVVIIEEYNLWKLKTVLIKSPNPSVQIVTEKLPKTEQLHKTEHPKQQQKEPSCWTT 303
            L L ++I        ++  +   P+    +++     T     +E   + +   S    
Sbjct: 261 MLALPLLIARRAQRDGMEKTV---PHDYSPLISSPKATTSGNQSSEGDSKVEAGLS---- 320

Query: 304 IFNPPARGEDFTILQALFSVDMLILFLSAICGVGGTLTAIDNLGQIGMSLGYPKRSISTF 363
                   E+  +LQA+  +   +LFL+ ICG+G  L+ I+N+ QIG SL Y    I++ 
Sbjct: 321 --------ENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIGESLRYSSVEINSL 380

Query: 364 VTLVSIWNYLGRVASGFISEIVLTKYKFPRPLMLSLTLLFSCIGHLMIAFDVPNGLYVAS 423
           V+L SIWN+LGR  +G+ S+ +L K  +PRPL+++ TL    IGHL+IA      LYV S
Sbjct: 381 VSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLIIASGFQGNLYVGS 440

Query: 424 VVIGFCFGAQWPLIFAIISELFGLKYYSTLFNFGSVASPIGLYVLNVKVAG 459
           V++G C+G+QW L+  I SELFG+++  T+FN  SVASPIG Y+ +V++ G
Sbjct: 441 VIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSVRLIG 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008446819.12.2e-23390.46PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0034695.1 ... [more]
XP_038893353.11.1e-23288.91LOW QUALITY PROTEIN: protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
XP_004142499.33.1e-23289.78protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis sativus] >KAE8648849.1 hypothet... [more]
XP_022956604.17.2e-22988.82protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata][more]
XP_023535761.12.1e-22888.65protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F4I9E13.2e-2225.00Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Q037952.3e-0421.46Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
A0A5D3CFW31.0e-23390.46Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3BG091.0e-23390.46protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103489435 ... [more]
A0A6J1GZI83.5e-22988.82protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1K1862.8e-22687.53protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489... [more]
A0A6J1FZF25.4e-22286.98protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucurbita moschata OX=3662 GN=LOC111449326... [more]
Match NameE-valueIdentityDescription
AT2G39210.17.5e-20075.27Major facilitator superfamily protein [more]
AT2G28120.19.6e-16361.82Major facilitator superfamily protein [more]
AT5G50630.18.9e-7637.23Major facilitator superfamily protein [more]
AT5G50520.18.9e-7637.23Major facilitator superfamily protein [more]
AT1G74780.11.1e-7335.88Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 220..456
e-value: 2.0E-7
score: 32.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 2..449
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 1..235
e-value: 1.5E-87
score: 293.2
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 1..459
NoneNo IPR availablePANTHERPTHR21576:SF117MFS TRANSPORTERcoord: 1..459
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 1..460
e-value: 2.3927E-99
score: 301.093

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC05G100940.1CaUC05G100940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0099587 inorganic ion import across plasma membrane
biological_process GO:0002239 response to oomycetes
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity