CaUC05G099130 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC05G099130
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionEnt-kaurene oxidase
LocationCiama_Chr05: 30196569 .. 30198845 (+)
RNA-Seq ExpressionCaUC05G099130
SyntenyCaUC05G099130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTTGTCACTGATCCTCTTGCTTCCATGCAGCTTCTCGCCAATACCCTTCAGGCTCCGCCCTATGCGGCAGCCGCCGTTCTGGGTGGCGTCTCTTTAATGCTTTCGGTGTTTCTTGTCGCTGATTGTAGAAAGAAACGTCGGAAATTTCTCCCTCCAGTGCCGGGTAATTATCGGCTTTTTGTCACTCTGTTTTCAATCATGGTGGAACTGATCGATTACTGTTTTGGATTCTCCTTCTAATTCTAGGGTTTTTAGGGAAAATGTTGTGAAATTGATGATCTGATGAATTTGGGAAATTTGATTTGGTTTTTGTAGCGATTCCGGGGGTGCCGCTGTTGGGAAATCTGTTGCAGTTGAAGGAGAAGAAGCCTCACATGACGTTTGCACGGTGGGCCGAGACGTACGGTCCGGTTTATTCCATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCGCCGAAGTTGCCAAAGAGGTTCGTAATTAAAATCACGGTTGTTTTCCGGCCAAAATTTTCCCTCTACCTCTGATTCTAACAGTGCTCTGTTCTGGTCTTGATTCTTTTAAGGCTATGGTGACGAGATACGCTTCTATTTCTAGTAGAAAGCTAAGCAAAGCCCTCACCATCTTGACTGCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGATGGTGAAGAGATACATATTGGCGAATGTCTTGGGGGCTAATGCTCAGGTTGTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTATTTTCATTTGGGTTAAATCTAATAGTTTGATCTGTTTCATTTTCAGAAGAAACACAGACAAAGGAGAGACACCATGATAGAGAATATTTCTAGGGAGTTGTTTGCTCATCTGAAGGATTTGCCATCAGAAACTGTTAATTTTCGAAAAATCTTCGAAGCAGAACTCTTTCGACTGGCTCTTAAAGAAGTGAGTTGATTCTGTTCTACAATTAAATTCAAAACTTGGATATGTGGATGGATAGAAAGGCGCAGGGAGTATGGATAAATAGCTATAACTTTCATGATTTCATTAACAGACACTTGGGAAAGATGTTGAGTCGATATATGTCGATGGGCTCGGTACCACATTGCCAAGGGAAGATCTGTTTAGGGTTTTAGTGATTGATCCAATGGAGGGTGCAATTGAGGTTGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAACAAGAGTGTGGAGAATAGAATAAAGAATATGGATTTTCGAAGGCGAATGACAATGAAAAGCCTACTTGAGGAGCAGAAGAAACGAATTGCAGCAGGGGAGGTAGTTCTTCCTTGTTTCTCTTGAATTGAAATGAGGCTGTTTATCTGCTTTGTTTGACTTAACTTGAACTTATAACAGGAAACGAATTGCTATGCTGATTTCCTGTTATCTGAAGCAAAAACACTTACAGAGGATCAGATATCGATGTTACTTTGGGAGACAATCATTGAAACCTCAGACACGACTCTGGTTGTAACAGAATGGGCTATGTATGAACTTGCAAAAGATCCGAAACGACAGGCAATTTCTGAACAACAATACCATTTTATGTTTTCTGCTTGTCTTTCCAATTCTTAAGATCATAAAAACTAGTCGACATTCTTTAAAAACTCTTGACATTGCAGGACTATCTGTATCAGCATATACTGAATGTCTGTGGATCTGCAAAGCTTACTGAAGAAAACCTGTCACAGCTTCCATATCTAACTGCTGTCTTTCATGAAACTCTTCGGAGGCATAGTCCAGTCCCGGTCGTCCCATTGCGATATGCGCATGAAGATACCCAGTTAGGCGGGTACTTTGTTCCTGCAGGGAGTGAGGTGAGCCATTCTAGAACCCTGGAACCTCTTAGTGTAATTTAATACGACAATGTCTGATGATCTCCGTTGTCAGATTGCTGTGAACATTTACGCGTGCAACATGGACAAGAATCATTGGGAAAGCCCTGAGGAATGGAACCCAGAGAGGTTTCTGGATGAGAAGTATGATCCAATGGATCTCCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTTAAGGCAATGCTGATAGCTTGCACAACCATTGGAAGGATGGTTCAAGAGTTTGAGTGGAGGCTAAGGGACGGGGAAGAGGACAAAGTGGACACTCTCGGCCTCACGGCTCGCAAGCTCCAGCCTCTGCACGTAGTCGTAAAGCCAAGAATCAACTAA

mRNA sequence

ATGGCGGTTGTCACTGATCCTCTTGCTTCCATGCAGCTTCTCGCCAATACCCTTCAGGCTCCGCCCTATGCGGCAGCCGCCGTTCTGGGTGGCGTCTCTTTAATGCTTTCGGTGTTTCTTGTCGCTGATTGTAGAAAGAAACGTCGGAAATTTCTCCCTCCAGTGCCGGGTAATTATCGGCTTTTTGTCACTCTGTTTTCAATCATGGTGGAACTGATCGATTACTCGATTCCGGGGGTGCCGCTGTTGGGAAATCTGTTGCAGTTGAAGGAGAAGAAGCCTCACATGACGTTTGCACGGTGGGCCGAGACGTACGGTCCGGTTTATTCCATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCGCCGAAGTTGCCAAAGAGGCTATGGTGACGAGATACGCTTCTATTTCTAGTAGAAAGCTAAGCAAAGCCCTCACCATCTTGACTGCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGATGGTGAAGAGATACATATTGGCGAATGTCTTGGGGGCTAATGCTCAGAAGAAACACAGACAAAGGAGAGACACCATGATAGAGAATATTTCTAGGGAGTTGTTTGCTCATCTGAAGGATTTGCCATCAGAAACTGTTAATTTTCGAAAAATCTTCGAAGCAGAACTCTTTCGACTGGCTCTTAAAGAAACACTTGGGAAAGATGTTGAGTCGATATATGTCGATGGGCTCGGTACCACATTGCCAAGGGAAGATCTGTTTAGGGTTTTAGTGATTGATCCAATGGAGGGTGCAATTGAGGTTGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAACAAGAGTGTGGAGAATAGAATAAAGAATATGGATTTTCGAAGGCGAATGACAATGAAAAGCCTACTTGAGGAGCAGAAGAAACGAATTGCAGCAGGGGAGGAAACGAATTGCTATGCTGATTTCCTGTTATCTGAAGCAAAAACACTTACAGAGGATCAGATATCGATGTTACTTTGGGAGACAATCATTGAAACCTCAGACACGACTCTGGTTGACTATCTGTATCAGCATATACTGAATGTCTGTGGATCTGCAAAGCTTACTGAAGAAAACCTGTCACAGCTTCCATATCTAACTGCTGTCTTTCATGAAACTCTTCGGAGGCATAGTCCAGTCCCGGTCGTCCCATTGCGATATGCGCATGAAGATACCCAGTTAGGCGGGTACTTTGTTCCTGCAGGGAGTGAGATTGCTGTGAACATTTACGCGTGCAACATGGACAAGAATCATTGGGAAAGCCCTGAGGAATGGAACCCAGAGAGGTTTCTGGATGAGAAGTATGATCCAATGGATCTCCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTTAAGGCAATGCTGATAGCTTGCACAACCATTGGAAGGATGGTTCAAGAGTTTGAGTGGAGGCTAAGGGACGGGGAAGAGGACAAAGTGGACACTCTCGGCCTCACGGCTCGCAAGCTCCAGCCTCTGCACGTAGTCGTAAAGCCAAGAATCAACTAA

Coding sequence (CDS)

ATGGCGGTTGTCACTGATCCTCTTGCTTCCATGCAGCTTCTCGCCAATACCCTTCAGGCTCCGCCCTATGCGGCAGCCGCCGTTCTGGGTGGCGTCTCTTTAATGCTTTCGGTGTTTCTTGTCGCTGATTGTAGAAAGAAACGTCGGAAATTTCTCCCTCCAGTGCCGGGTAATTATCGGCTTTTTGTCACTCTGTTTTCAATCATGGTGGAACTGATCGATTACTCGATTCCGGGGGTGCCGCTGTTGGGAAATCTGTTGCAGTTGAAGGAGAAGAAGCCTCACATGACGTTTGCACGGTGGGCCGAGACGTACGGTCCGGTTTATTCCATAAGAACCGGCGCTTCCACTGTGATTGTTCTCAACACCGCCGAAGTTGCCAAAGAGGCTATGGTGACGAGATACGCTTCTATTTCTAGTAGAAAGCTAAGCAAAGCCCTCACCATCTTGACTGCTGATAAATGCATGGTTGCTATGAGTGATTACAATGACTTTCACAAGATGGTGAAGAGATACATATTGGCGAATGTCTTGGGGGCTAATGCTCAGAAGAAACACAGACAAAGGAGAGACACCATGATAGAGAATATTTCTAGGGAGTTGTTTGCTCATCTGAAGGATTTGCCATCAGAAACTGTTAATTTTCGAAAAATCTTCGAAGCAGAACTCTTTCGACTGGCTCTTAAAGAAACACTTGGGAAAGATGTTGAGTCGATATATGTCGATGGGCTCGGTACCACATTGCCAAGGGAAGATCTGTTTAGGGTTTTAGTGATTGATCCAATGGAGGGTGCAATTGAGGTTGATTGGAGGGATTTCTTCCCATATCTGAGATGGATTCCCAACAAGAGTGTGGAGAATAGAATAAAGAATATGGATTTTCGAAGGCGAATGACAATGAAAAGCCTACTTGAGGAGCAGAAGAAACGAATTGCAGCAGGGGAGGAAACGAATTGCTATGCTGATTTCCTGTTATCTGAAGCAAAAACACTTACAGAGGATCAGATATCGATGTTACTTTGGGAGACAATCATTGAAACCTCAGACACGACTCTGGTTGACTATCTGTATCAGCATATACTGAATGTCTGTGGATCTGCAAAGCTTACTGAAGAAAACCTGTCACAGCTTCCATATCTAACTGCTGTCTTTCATGAAACTCTTCGGAGGCATAGTCCAGTCCCGGTCGTCCCATTGCGATATGCGCATGAAGATACCCAGTTAGGCGGGTACTTTGTTCCTGCAGGGAGTGAGATTGCTGTGAACATTTACGCGTGCAACATGGACAAGAATCATTGGGAAAGCCCTGAGGAATGGAACCCAGAGAGGTTTCTGGATGAGAAGTATGATCCAATGGATCTCCACAAAACCATGGCCTTTGGAGGAGGGAAGAGGGTGTGTGCAGGTGCTCTTAAGGCAATGCTGATAGCTTGCACAACCATTGGAAGGATGGTTCAAGAGTTTGAGTGGAGGCTAAGGGACGGGGAAGAGGACAAAGTGGACACTCTCGGCCTCACGGCTCGCAAGCTCCAGCCTCTGCACGTAGTCGTAAAGCCAAGAATCAACTAA

Protein sequence

MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYRLFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVDYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN
Homology
BLAST of CaUC05G099130 vs. NCBI nr
Match: XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])

HSP 1 Score: 936.4 bits (2419), Expect = 1.1e-268
Identity = 472/538 (87.73%), Postives = 495/538 (92.01%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MAVVTDPL SMQLLANTLQAPPYAAAAVLGGVSL+LSVFLVADCRKKRR FLPPVP    
Sbjct: 1   MAVVTDPLTSMQLLANTLQAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          +IPGVP+LGNLLQLKEKKPHMTFARWAE YGPVYSIRTGASTVIV
Sbjct: 61  ---------------AIPGVPVLGNLLQLKEKKPHMTFARWAEKYGPVYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILAN+LGA
Sbjct: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQKKHRQRRDTMIENISRELFAH+K+ PSE+VNFRKIFE+ELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKKHRQRRDTMIENISRELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVEN+I+ MDF+R+MTM
Sbjct: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENKIRKMDFQRQMTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
           +SL+EEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV       
Sbjct: 301 RSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    D+LYQHIL+ CGS+KLTEENLSQLPYLTAVFHETLR+HSPVPVVPLRYAHE
Sbjct: 361 ELAKDPKRQDFLYQHILSACGSSKLTEENLSQLPYLTAVFHETLRKHSPVPVVPLRYAHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           DTQLGG+FVPAGSEIAVNIYACNMDK+HWESPEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DTQLGGFFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACTTIGRM QEFEW+LR+GEEDKVDTLGLTARKLQPLHVVVKPRIN
Sbjct: 481 RVCAGALKAMLIACTTIGRMAQEFEWKLREGEEDKVDTLGLTARKLQPLHVVVKPRIN 519

BLAST of CaUC05G099130 vs. NCBI nr
Match: XP_008452488.1 (PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-kaurene oxidase [Cucumis melo var. makuwa] >TYK20237.1 ent-kaurene oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 927.2 bits (2395), Expect = 6.4e-266
Identity = 466/538 (86.62%), Postives = 490/538 (91.08%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MAVVTDPLASMQLLANTL APPYAAAAVLGGVSL+LSVFLVADCRKKRR FLPPVP    
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          ++PGVPLLGNLLQLKEKKPH TFARWAETYGPVYSIRTGASTVIV
Sbjct: 61  ---------------AVPGVPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNT EVAKEAMVTRY SISSRKLSKALTILTADKCMVAMSDYN+FHKMVKRYILANVLGA
Sbjct: 121 LNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQKKHRQRRD MIENISRELFAH+K+ PS+TVNFRKIFE+ELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKKHRQRRDAMIENISRELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFR+LVIDPMEGAIEVDWRDFFPYLRWIPNKSVEN+I+NMDFRRRMTM
Sbjct: 241 VDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
           KSL++E K+RIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV       
Sbjct: 301 KSLIQEPKRRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQ I +VCGS+ LTEENLSQLPYLTA+FHETLR+HSPVPVVPLRYAHE
Sbjct: 361 ELSKDPKRQDYLYQQIQSVCGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           DTQLGGYFVPAGSEIAVNIYACNMDKNHWE+PEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACTTIGRMVQEFEW+LR+GEE+KVDTLGLTARKLQPLHVV+KPR N
Sbjct: 481 RVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

BLAST of CaUC05G099130 vs. NCBI nr
Match: NP_001267703.1 (ent-kaurene oxidase, chloroplastic-like [Cucumis sativus] >AFH03045.1 ent-kaurene oxidase [Cucumis sativus] >AFQ32799.1 ent-kaurene oxidase [Cucumis sativus] >KGN55152.1 hypothetical protein Csa_012319 [Cucumis sativus])

HSP 1 Score: 914.4 bits (2362), Expect = 4.3e-262
Identity = 461/538 (85.69%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MAVVTDPLASMQLLANT+ APPYAAAAVLGGVSL+LSVF VADCRKKRR FLPPVP    
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          ++PGVP+LGNLLQLKEKKPH TFARWAETYG VYSIRTGASTVIV
Sbjct: 61  ---------------AVPGVPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNT EVAKEAMVTRY SISSRKLSKALTILTADKCMVAMSDYN+FHKMVKRYILANVLGA
Sbjct: 121 LNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQKKHRQRRD MIENISRELFAH+K+ P +TVNFRKIFEAELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFR+LVIDPMEGAIEVDWRDFFPYLRWIPNK VEN+I+NMDFRRRMTM
Sbjct: 241 VDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
           K L+EE KKRIAAGEET CYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV       
Sbjct: 301 KKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQ I +VCGSA LTEENLSQLPYLTA+FHETLR+HSPVPVVPLRYAHE
Sbjct: 361 ELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           DTQLGGYFVPAGSEIAVNIYACNMDK+HWESPEEW PERFLD+KYDPMDLHKTMAFGGGK
Sbjct: 421 DTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACTTIGRMVQEFEW+LR+GEE+KVDTLGLTARKLQPLHVV+KPR N
Sbjct: 481 RVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

BLAST of CaUC05G099130 vs. NCBI nr
Match: KAG7024228.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 871.3 bits (2250), Expect = 4.2e-249
Identity = 432/522 (82.76%), Postives = 475/522 (91.00%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MA+  DPL S+Q L +TLQAP YAAA++LG +S +L+ FLVAD RK RR FLPPVP    
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          +IPG+P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTGASTVIV
Sbjct: 61  ---------------AIPGLPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNTAEVAKEAMVTRYAS+SSRKLSKALTILT+DKCMVAMSDYNDFHKMVK+YILANVLGA
Sbjct: 121 LNTAEVAKEAMVTRYASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQK+HRQRRDTMI+NIS +LFAH+KD PSE+VNFR+IFE+ELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKRHRQRRDTMIDNISSQLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLG TLPREDLFRVLVIDPMEGAIEVDWRDFFPYL+WIPNKSVEN+I+ MDF RR+TM
Sbjct: 241 VDGLG-TLPREDLFRVLVIDPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFCRRVTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVDYLYQHI 360
            SL+EEQKKRIAAGEETNCYADFLLSEAKTLTE QI+MLLWETIIETSDTTLVDYLYQHI
Sbjct: 301 MSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVDYLYQHI 360

Query: 361 LNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGSEIA 420
            +VCGS KLTE++LS+LPYLTAVFHETLR+HSPVP+VPLRY HED QLGG+FVPAGSEIA
Sbjct: 361 HSVCGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHEDAQLGGFFVPAGSEIA 420

Query: 421 VNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTT 480
           VNIYACNMDK+ WESPEEW PERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT+
Sbjct: 421 VNIYACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACTS 480

Query: 481 IGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           IGR+VQEFEW+L +GEEDKVDTLGLTARKLQPLH +VKPRI+
Sbjct: 481 IGRLVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPRIS 502

BLAST of CaUC05G099130 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 867.1 bits (2239), Expect = 7.9e-248
Identity = 433/538 (80.48%), Postives = 476/538 (88.48%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MA+  DPL S+Q L +TLQAPPYAAAA+LG +S +L+ FLVAD RK RR FLPPVP    
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          +IPG+P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTGASTVIV
Sbjct: 61  ---------------AIPGLPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNTAEVAKEAMVTRYAS+S RKLSKALTILT+DKCMVAMSDYNDFHKMVK+YILANVLGA
Sbjct: 121 LNTAEVAKEAMVTRYASMSGRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQK+HRQRRD+MI+NIS +LFAH+KD PSE+VNFRKIFE+ELF LALKETLGKD+ESIY
Sbjct: 181 NAQKRHRQRRDSMIDNISSQLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFRVLVID MEGAIEVDWRDFFPYL+WIPNKSVEN+I+ MD+RRR+TM
Sbjct: 241 VDGLGTTLPREDLFRVLVIDLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
            SL+EEQKKRIAAGEETNCYADFLLSEAKTLTE QI+MLLWETIIETSDTTLV       
Sbjct: 301 MSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQHI +VCGS KLTE++LS+LPYLTAVFHETLR+HSPVP+VPLRY HE
Sbjct: 361 ELAKNPERQDYLYQHIQSVCGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           D QLGG+FVPAGSEIAVNIYACNMDK+ WESPEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DAQLGGFFVPAGSEIAVNIYACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACT+IGR+VQEFEW+L +GEEDKVDTLGLTARKLQPLHV+VKPRI+
Sbjct: 481 RVCAGALKAMLIACTSIGRLVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPRIS 519

BLAST of CaUC05G099130 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.6e-179
Identity = 317/527 (60.15%), Postives = 387/527 (73.43%), Query Frame = 0

Query: 17  TLQAPPYAAA-----AVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYRLFVTLFSIMVE 76
           +LQA P AAA       +GG++L      V D RKK   F PP                 
Sbjct: 6   SLQAVPAAAAIGGPVVAIGGITLFFIREYVKDQRKKSSSFPPPP---------------- 65

Query: 77  LIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAM 136
                +PG+P++GNLLQLKEKKPH TF +W+E YGP+YSIRTGA+T+IVLNT +VAKEAM
Sbjct: 66  ----KVPGLPVIGNLLQLKEKKPHKTFTKWSEKYGPIYSIRTGANTMIVLNTNDVAKEAM 125

Query: 137 VTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRD 196
           +T+Y+SIS+RKLSKALTILT+DK +VAMSDYN+F+K  KR++L + LG  AQK+HR  R+
Sbjct: 126 ITKYSSISTRKLSKALTILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRN 185

Query: 197 TMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPRE 256
            MI NI  +  AH K  PSE VNFRKIF++ELF L++K+ +G+DVESIYV+ L TTL R+
Sbjct: 186 LMINNICDQFLAHAKMYPSEAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQ 245

Query: 257 DLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRI 316
           ++F++LV+DPMEGAI+VDWRDFFPYL+WIPN+  EN+I+ M F R   MK+L+E+QKKRI
Sbjct: 246 EMFKILVVDPMEGAIDVDWRDFFPYLKWIPNQHFENKIQQMHFHREAVMKALIEQQKKRI 305

Query: 317 AAGEETNCYADFLLSE-AKTLTEDQISMLLWETIIETSDTTLV----------------D 376
           A+G+  NCY D LLSE A TL+E QI MLLWE IIE SDTTLV                +
Sbjct: 306 ASGKAINCYLDHLLSEAADTLSEQQILMLLWEAIIEASDTTLVTTEWAMYELSKDPKRQN 365

Query: 377 YLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVP 436
           YL   I N CG  +L EE L +LPYL A+F ETLR+HSPVPVVPLRY HE+TQLGGY +P
Sbjct: 366 YLLSEIQNACGFDQLNEEKLCRLPYLAAIFQETLRKHSPVPVVPLRYVHEETQLGGYTIP 425

Query: 437 AGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAM 496
            GSEIA+NIY CNMDKN W+SPEEW PERF+  K D  +LHKTMAFGGGKRVCAGAL+AM
Sbjct: 426 EGSEIAINIYGCNMDKNVWDSPEEWRPERFVFGKDDTTELHKTMAFGGGKRVCAGALQAM 485

Query: 497 LIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRI 522
            I+C  IGR+VQE EWRL DGEE  VDTLGLT  KL PL  ++KPR+
Sbjct: 486 TISCIAIGRLVQELEWRLGDGEEANVDTLGLTTHKLHPLQTIIKPRL 512

BLAST of CaUC05G099130 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 597.8 bits (1540), Expect = 1.2e-169
Identity = 291/461 (63.12%), Postives = 361/461 (78.31%), Query Frame = 0

Query: 77  IPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYA 136
           +PG P++GNLLQLKEKKPH TF RW+E YGP+YSI+ G+S++IVLN+ E AKEAMVTR++
Sbjct: 47  VPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEAMVTRFS 106

Query: 137 SISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMIEN 196
           SIS+RKLS ALT+LT DK MVA SDY+DFHK+VKR +L  +LGANAQK+ R  RD +IEN
Sbjct: 107 SISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYRDALIEN 166

Query: 197 ISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFRV 256
           +S +L AH +D P E VNFR IFE ELF +ALK+  GKDVESIYV  LG TL ++++F+V
Sbjct: 167 VSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSKDEIFKV 226

Query: 257 LVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRIAAGEE 316
           LV D MEGAI+VDWRDFFPYL+WIPNKS E RI+    RR   M +L++++ K+  +  +
Sbjct: 227 LVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQNGSESD 286

Query: 317 TNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHI 376
            +CY +FL+SEAKTLT++QI++L+WETIIET+DTTLV                D L + I
Sbjct: 287 DDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQDRLCKEI 346

Query: 377 LNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGSEIA 436
            NVCG  K  EE LSQ+PYL  VFHETLR++SP P+VP+RYAHEDTQ+GGY VPAGSEIA
Sbjct: 347 QNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVPAGSEIA 406

Query: 437 VNIYACNMDKNHWESPEEWNPERFLDE-KYDPMDLHKTMAFGGGKRVCAGALKAMLIACT 496
           +NIY CNMDK  WE PE+W PERFLD+ KY+  DLHKTMAFG GKRVCAGAL+A L+A  
Sbjct: 407 INIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQASLMAGI 466

Query: 497 TIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPR 521
            IGR+VQEFEW+LRDGEE+ VDT GLT++KL PL  ++ PR
Sbjct: 467 AIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

BLAST of CaUC05G099130 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.3e-152
Identity = 259/456 (56.80%), Postives = 340/456 (74.56%), Query Frame = 0

Query: 76  SIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRY 135
           ++PG+P++GNL QLKEKKPH TFA+WAE YGP+Y+IRTGAS+V+VLN+ EVAKEAMV ++
Sbjct: 43  AVPGLPIIGNLHQLKEKKPHQTFAKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKF 102

Query: 136 ASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMIE 195
           +SIS+RKLSKALT+LT DK MVA SDY DFHKMVKRY+++++LG +AQK+ R  RD MI 
Sbjct: 103 SSISTRKLSKALTVLTRDKSMVATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIH 162

Query: 196 NISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFR 255
           N+       +KD P   + FR +F+ ELFRL++ ++LG+DV S+YVD  G  + +E+++ 
Sbjct: 163 NMLSTFHKLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYN 222

Query: 256 VLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRIAAGE 315
             V D M  AIEVDWRDFFPYL W+PNKS E R+   + RR   M++L+++QK+RI  GE
Sbjct: 223 ATVTDMMMCAIEVDWRDFFPYLSWVPNKSFETRVFTTETRRTAVMRALIKQQKERIVRGE 282

Query: 316 ETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQH 375
              CY DFLL+E  TLT++Q+ ML+WE +IE +DTTLV                + LYQ 
Sbjct: 283 AKTCYLDFLLAE-NTLTDEQLMMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQE 342

Query: 376 ILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGSEI 435
           I  VCG   +TEE+L +LPYL AVFHETLRRHSPVP++P R+ HEDT+L GY VPAG+E+
Sbjct: 343 IREVCGDETVTEEHLPRLPYLNAVFHETLRRHSPVPLIPPRFVHEDTKLAGYDVPAGTEM 402

Query: 436 AVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT 495
            +N+Y CNM++  WESPEEW PERF   + +  D++KTMAFG G+R CAG+L+A  IAC 
Sbjct: 403 VINLYGCNMNRKEWESPEEWVPERFAGGRLEVADMYKTMAFGAGRRACAGSLQATHIACA 462

Query: 496 TIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHV 516
            + R VQEF WRLR+G+E+KVDT+ LTA KL PLHV
Sbjct: 463 AVARFVQEFGWRLREGDEEKVDTVQLTAYKLHPLHV 497

BLAST of CaUC05G099130 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.3e-149
Identity = 258/461 (55.97%), Postives = 337/461 (73.10%), Query Frame = 0

Query: 76  SIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRY 135
           ++PG+P++GNL QLKEKKPH TF +WAE YGP+Y+IRTGAS+V+VLN+ EVAKEAMV ++
Sbjct: 42  AVPGLPIIGNLHQLKEKKPHQTFTKWAEIYGPIYTIRTGASSVVVLNSTEVAKEAMVAKF 101

Query: 136 ASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMIE 195
           +SIS+RKLSKALT+L+ DK MVA SD  DFHKM KRYI+ ++LG +AQK+ R  RD +I 
Sbjct: 102 SSISTRKLSKALTVLSHDKSMVATSDSGDFHKMGKRYIMLSMLGTSAQKQFRDTRDMIIN 161

Query: 196 NISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFR 255
           N+       +KD P   + FR +F+ ELFRL++ ++LG+DV S+YVD  G  + +E+++ 
Sbjct: 162 NMLSTFHQLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYN 221

Query: 256 VLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRIAAGE 315
             V D M  AIEVDWRDFFPYL W+PNKS E R+   + RR   M++L+++QK+RI  GE
Sbjct: 222 ATVTDMMMCAIEVDWRDFFPYLSWVPNKSFETRVFTTESRRTAVMRALIKQQKERIVRGE 281

Query: 316 ETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQH 375
              CY DFLL+E  TLT++Q+ ML+WE +IE +DTTLV                + LYQ 
Sbjct: 282 ARTCYLDFLLAE-NTLTDEQLMMLVWEALIEAADTTLVTTEWAMYELAKNPDKQERLYQE 341

Query: 376 ILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGSEI 435
           I  VCG   +TEE+L  LPYL AVF ETLRRHSPVP++P R+ +EDT L GY VPAG+E+
Sbjct: 342 IREVCGDEAVTEEHLPWLPYLNAVFQETLRRHSPVPLIPPRFVNEDTMLAGYDVPAGTEM 401

Query: 436 AVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIACT 495
            +N+Y CNM+K  WESPEEW PERF   ++   D++KTMAFG G+RVCAG+L+A  IAC 
Sbjct: 402 VINLYGCNMNKKEWESPEEWAPERFAGGRFKVADMYKTMAFGAGRRVCAGSLQATHIACA 461

Query: 496 TIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPR 521
            I R VQEF WRLR+G+E+KVDT+ LTA KL PLHV + PR
Sbjct: 462 AIARFVQEFGWRLREGDEEKVDTVQLTAYKLHPLHVHLTPR 501

BLAST of CaUC05G099130 vs. ExPASy Swiss-Prot
Match: Q0DBF4 (Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A8 PE=1 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.5e-137
Identity = 239/463 (51.62%), Postives = 330/463 (71.27%), Query Frame = 0

Query: 76  SIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRY 135
           ++PG+P++GNL QLKEKKPH TFA+W+ETYGP+Y+I+TGAS V+VLN+ EVAKEAM+ ++
Sbjct: 46  AVPGLPIIGNLHQLKEKKPHQTFAKWSETYGPIYTIKTGASPVVVLNSTEVAKEAMIDKF 105

Query: 136 ASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMIE 195
           +SIS+RKL KA+++LT  K MVA+SDY D+ KM KR I+  +LG NAQK+ R  R+ MI 
Sbjct: 106 SSISTRKLPKAMSVLTR-KSMVAISDYGDYQKMAKRNIMIGMLGFNAQKQFRGTRERMIS 165

Query: 196 NISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFR 255
           N+   L   +   P   +NFR ++  ELF L+L ++LG+DV S+YV+  G  + ++++F 
Sbjct: 166 NVLSTLHKLVSLDPHSPLNFRDVYINELFSLSLIQSLGEDVSSVYVEEFGREISKDEIFD 225

Query: 256 VLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRIAAGE 315
           VLV + M  A+E DWRD+FPYL W+PNKS +  +   +FRR   M +L+++QK+RIA GE
Sbjct: 226 VLVHEMMMCAVEADWRDYFPYLSWLPNKSFDTIVSTTEFRRDAIMNALIKKQKERIARGE 285

Query: 316 ETNCYADFLLSEAKT--LTEDQISMLLWETIIETSDTTLV----------------DYLY 375
               Y DFLL   ++  LT+DQ+ +LL E+I+  +DT LV                + LY
Sbjct: 286 ARASYIDFLLEAERSAQLTDDQLMLLLSESILAAADTVLVTTEWTMYEIAKNPDKQELLY 345

Query: 376 QHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGS 435
           Q I   CG   +TE++L +LPYL AVFHETLR HSPVPV+P R+ H+DT L GY + AG+
Sbjct: 346 QEIREACGGEAVTEDDLPRLPYLNAVFHETLRLHSPVPVLPPRFVHDDTTLAGYDIAAGT 405

Query: 436 EIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGKRVCAGALKAMLIA 495
           ++ +N+YAC+MD+  WESP EW+PERFL E ++  D +KTMAFG G+R CAG+L+AM IA
Sbjct: 406 QMMINVYACHMDEKVWESPGEWSPERFLGEGFEVADRYKTMAFGAGRRTCAGSLQAMNIA 465

Query: 496 CTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPR 521
           C  + R+VQE EWRLR+G+ DK DT+  TA KL PLHV +KPR
Sbjct: 466 CVAVARLVQELEWRLREGDGDKEDTMQFTALKLDPLHVHLKPR 507

BLAST of CaUC05G099130 vs. ExPASy TrEMBL
Match: A0A5D3D9I5 (Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003760 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 3.1e-266
Identity = 466/538 (86.62%), Postives = 490/538 (91.08%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MAVVTDPLASMQLLANTL APPYAAAAVLGGVSL+LSVFLVADCRKKRR FLPPVP    
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          ++PGVPLLGNLLQLKEKKPH TFARWAETYGPVYSIRTGASTVIV
Sbjct: 61  ---------------AVPGVPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNT EVAKEAMVTRY SISSRKLSKALTILTADKCMVAMSDYN+FHKMVKRYILANVLGA
Sbjct: 121 LNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQKKHRQRRD MIENISRELFAH+K+ PS+TVNFRKIFE+ELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKKHRQRRDAMIENISRELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFR+LVIDPMEGAIEVDWRDFFPYLRWIPNKSVEN+I+NMDFRRRMTM
Sbjct: 241 VDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
           KSL++E K+RIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV       
Sbjct: 301 KSLIQEPKRRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQ I +VCGS+ LTEENLSQLPYLTA+FHETLR+HSPVPVVPLRYAHE
Sbjct: 361 ELSKDPKRQDYLYQQIQSVCGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           DTQLGGYFVPAGSEIAVNIYACNMDKNHWE+PEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACTTIGRMVQEFEW+LR+GEE+KVDTLGLTARKLQPLHVV+KPR N
Sbjct: 481 RVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

BLAST of CaUC05G099130 vs. ExPASy TrEMBL
Match: A0A1S3BTZ0 (ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 3.1e-266
Identity = 466/538 (86.62%), Postives = 490/538 (91.08%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MAVVTDPLASMQLLANTL APPYAAAAVLGGVSL+LSVFLVADCRKKRR FLPPVP    
Sbjct: 1   MAVVTDPLASMQLLANTLPAPPYAAAAVLGGVSLVLSVFLVADCRKKRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          ++PGVPLLGNLLQLKEKKPH TFARWAETYGPVYSIRTGASTVIV
Sbjct: 61  ---------------AVPGVPLLGNLLQLKEKKPHKTFARWAETYGPVYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNT EVAKEAMVTRY SISSRKLSKALTILTADKCMVAMSDYN+FHKMVKRYILANVLGA
Sbjct: 121 LNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQKKHRQRRD MIENISRELFAH+K+ PS+TVNFRKIFE+ELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKKHRQRRDAMIENISRELFAHVKEFPSDTVNFRKIFESELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFR+LVIDPMEGAIEVDWRDFFPYLRWIPNKSVEN+I+NMDFRRRMTM
Sbjct: 241 VDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKSVENKIRNMDFRRRMTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
           KSL++E K+RIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV       
Sbjct: 301 KSLIQEPKRRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQ I +VCGS+ LTEENLSQLPYLTA+FHETLR+HSPVPVVPLRYAHE
Sbjct: 361 ELSKDPKRQDYLYQQIQSVCGSSTLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           DTQLGGYFVPAGSEIAVNIYACNMDKNHWE+PEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWENPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACTTIGRMVQEFEW+LR+GEE+KVDTLGLTARKLQPLHVV+KPR N
Sbjct: 481 RVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

BLAST of CaUC05G099130 vs. ExPASy TrEMBL
Match: J7I3T1 (Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.1e-262
Identity = 461/538 (85.69%), Postives = 484/538 (89.96%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MAVVTDPLASMQLLANT+ APPYAAAAVLGGVSL+LSVF VADCRKKRR FLPPVP    
Sbjct: 1   MAVVTDPLASMQLLANTIPAPPYAAAAVLGGVSLVLSVFFVADCRKKRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          ++PGVP+LGNLLQLKEKKPH TFARWAETYG VYSIRTGASTVIV
Sbjct: 61  ---------------AVPGVPVLGNLLQLKEKKPHKTFARWAETYGAVYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNT EVAKEAMVTRY SISSRKLSKALTILTADKCMVAMSDYN+FHKMVKRYILANVLGA
Sbjct: 121 LNTTEVAKEAMVTRYGSISSRKLSKALTILTADKCMVAMSDYNEFHKMVKRYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQKKHRQRRD MIENISRELFAH+K+ P +TVNFRKIFEAELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKKHRQRRDAMIENISRELFAHVKEFPLDTVNFRKIFEAELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFR+LVIDPMEGAIEVDWRDFFPYLRWIPNK VEN+I+NMDFRRRMTM
Sbjct: 241 VDGLGTTLPREDLFRILVIDPMEGAIEVDWRDFFPYLRWIPNKRVENKIRNMDFRRRMTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
           K L+EE KKRIAAGEET CYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV       
Sbjct: 301 KKLMEEPKKRIAAGEETYCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQ I +VCGSA LTEENLSQLPYLTA+FHETLR+HSPVPVVPLRYAHE
Sbjct: 361 ELSKDPRRQDYLYQQIQSVCGSATLTEENLSQLPYLTAIFHETLRKHSPVPVVPLRYAHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           DTQLGGYFVPAGSEIAVNIYACNMDK+HWESPEEW PERFLD+KYDPMDLHKTMAFGGGK
Sbjct: 421 DTQLGGYFVPAGSEIAVNIYACNMDKDHWESPEEWKPERFLDDKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACTTIGRMVQEFEW+LR+GEE+KVDTLGLTARKLQPLHVV+KPR N
Sbjct: 481 RVCAGALKAMLIACTTIGRMVQEFEWKLREGEEEKVDTLGLTARKLQPLHVVIKPRNN 519

BLAST of CaUC05G099130 vs. ExPASy TrEMBL
Match: A0A6J1IL06 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476578 PE=3 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 3.8e-248
Identity = 433/538 (80.48%), Postives = 476/538 (88.48%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           MA+  DPL S+Q L +TLQAPPYAAAA+LG +S +L+ FLVAD RK RR FLPPVP    
Sbjct: 1   MAIFNDPLGSLQHLLDTLQAPPYAAAAILGVISFLLAGFLVADWRKSRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          +IPG+P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTGASTVIV
Sbjct: 61  ---------------AIPGLPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNTAEVAKEAMVTRYAS+S RKLSKALTILT+DKCMVAMSDYNDFHKMVK+YILANVLGA
Sbjct: 121 LNTAEVAKEAMVTRYASMSGRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQK+HRQRRD+MI+NIS +LFAH+KD PSE+VNFRKIFE+ELF LALKETLGKD+ESIY
Sbjct: 181 NAQKRHRQRRDSMIDNISSQLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLGTTLPREDLFRVLVID MEGAIEVDWRDFFPYL+WIPNKSVEN+I+ MD+RRR+TM
Sbjct: 241 VDGLGTTLPREDLFRVLVIDLMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDYRRRVTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
            SL+EEQKKRIAAGEETNCYADFLLSEAKTLTE QI+MLLWETIIETSDTTLV       
Sbjct: 301 MSLIEEQKKRIAAGEETNCYADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQHI +VCGS KLTE++LS+LPYLTAVFHETLR+HSPVP+VPLRY HE
Sbjct: 361 ELAKNPERQDYLYQHIQSVCGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           D QLGG+FVPAGSEIAVNIYACNMDK+ WESPEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DAQLGGFFVPAGSEIAVNIYACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACT+IGR+VQEFEW+L +GEEDKVDTLGLTARKLQPLHV+VKPRI+
Sbjct: 481 RVCAGALKAMLIACTSIGRLVQEFEWKLGEGEEDKVDTLGLTARKLQPLHVIVKPRIS 519

BLAST of CaUC05G099130 vs. ExPASy TrEMBL
Match: A0A6J1F7C8 (ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111442772 PE=3 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 1.0e-245
Identity = 430/538 (79.93%), Postives = 474/538 (88.10%), Query Frame = 0

Query: 1   MAVVTDPLASMQLLANTLQAPPYAAAAVLGGVSLMLSVFLVADCRKKRRKFLPPVPGNYR 60
           M +  DPL S+Q L +TLQAP YAAA++LG +S +L+ FLVAD RK RR FLPPVP    
Sbjct: 1   MVIFNDPLGSLQHLLDTLQAPTYAAASILGVISFLLAGFLVADWRKSRRNFLPPVP---- 60

Query: 61  LFVTLFSIMVELIDYSIPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIV 120
                          +IPG+P+LGNLLQLKEKKPH TFARWAETYGP+YSIRTGASTVIV
Sbjct: 61  ---------------AIPGLPVLGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIV 120

Query: 121 LNTAEVAKEAMVTRYASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGA 180
           LNTAEVAKEAMVTRYAS+SSRKLSKALTILT+DKCMVAMSDYNDFHKMVK+YILANVLGA
Sbjct: 121 LNTAEVAKEAMVTRYASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGA 180

Query: 181 NAQKKHRQRRDTMIENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIY 240
           NAQK+HRQRRDTMI+NIS +LFAH+KD PSE+VNFR+IFE+ELFRLALKETLGKD+ESIY
Sbjct: 181 NAQKRHRQRRDTMIDNISSQLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIY 240

Query: 241 VDGLGTTLPREDLFRVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTM 300
           VDGLG  LPREDLFRVLVIDPMEGAIEVDWRDFFPYL+WIPNKSVEN+I+ MDFRRR+TM
Sbjct: 241 VDGLG-MLPREDLFRVLVIDPMEGAIEVDWRDFFPYLKWIPNKSVENKIRQMDFRRRVTM 300

Query: 301 KSLLEEQKKRIAAGEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV------- 360
            SL+E+QKKRIAAGEETNCYADFLLSEAKTLTE QI+MLLWETIIETSDTTLV       
Sbjct: 301 MSLIEKQKKRIAAGEETNCYADFLLSEAKTLTEHQIAMLLWETIIETSDTTLVVTEWAMY 360

Query: 361 ---------DYLYQHILNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHE 420
                    DYLYQHI +VCGS KLTE++LS+LPYLTAVFHETLR+HSPVP+VPLRY HE
Sbjct: 361 ELAKNPKRQDYLYQHIQSVCGSEKLTEDDLSKLPYLTAVFHETLRKHSPVPIVPLRYVHE 420

Query: 421 DTQLGGYFVPAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLHKTMAFGGGK 480
           D QLGG+FVPAGSEIAVNIYACNMDK+ WESPEEW PERFLDEKYDPMDLHKTMAFGGGK
Sbjct: 421 DAQLGGFFVPAGSEIAVNIYACNMDKDRWESPEEWKPERFLDEKYDPMDLHKTMAFGGGK 480

Query: 481 RVCAGALKAMLIACTTIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPRIN 523
           RVCAGALKAMLIACT+IGR+VQEFEW+L +GEEDKVDTLGLTARKLQPLH +VKPRI+
Sbjct: 481 RVCAGALKAMLIACTSIGRLVQEFEWKLGEGEEDKVDTLGLTARKLQPLHAIVKPRIS 518

BLAST of CaUC05G099130 vs. TAIR 10
Match: AT5G25900.1 (GA requiring 3 )

HSP 1 Score: 597.8 bits (1540), Expect = 8.3e-171
Identity = 291/461 (63.12%), Postives = 361/461 (78.31%), Query Frame = 0

Query: 77  IPGVPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYA 136
           +PG P++GNLLQLKEKKPH TF RW+E YGP+YSI+ G+S++IVLN+ E AKEAMVTR++
Sbjct: 47  VPGFPVIGNLLQLKEKKPHKTFTRWSEIYGPIYSIKMGSSSLIVLNSTETAKEAMVTRFS 106

Query: 137 SISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMIEN 196
           SIS+RKLS ALT+LT DK MVA SDY+DFHK+VKR +L  +LGANAQK+ R  RD +IEN
Sbjct: 107 SISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYRDALIEN 166

Query: 197 ISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFRV 256
           +S +L AH +D P E VNFR IFE ELF +ALK+  GKDVESIYV  LG TL ++++F+V
Sbjct: 167 VSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSKDEIFKV 226

Query: 257 LVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQKKRIAAGEE 316
           LV D MEGAI+VDWRDFFPYL+WIPNKS E RI+    RR   M +L++++ K+  +  +
Sbjct: 227 LVHDMMEGAIDVDWRDFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLKQNGSESD 286

Query: 317 TNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLYQHI 376
            +CY +FL+SEAKTLT++QI++L+WETIIET+DTTLV                D L + I
Sbjct: 287 DDCYLNFLMSEAKTLTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQDRLCKEI 346

Query: 377 LNVCGSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAGSEIA 436
            NVCG  K  EE LSQ+PYL  VFHETLR++SP P+VP+RYAHEDTQ+GGY VPAGSEIA
Sbjct: 347 QNVCGGEKFKEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVPAGSEIA 406

Query: 437 VNIYACNMDKNHWESPEEWNPERFLDE-KYDPMDLHKTMAFGGGKRVCAGALKAMLIACT 496
           +NIY CNMDK  WE PE+W PERFLD+ KY+  DLHKTMAFG GKRVCAGAL+A L+A  
Sbjct: 407 INIYGCNMDKKRWERPEDWWPERFLDDGKYETSDLHKTMAFGAGKRVCAGALQASLMAGI 466

Query: 497 TIGRMVQEFEWRLRDGEEDKVDTLGLTARKLQPLHVVVKPR 521
            IGR+VQEFEW+LRDGEE+ VDT GLT++KL PL  ++ PR
Sbjct: 467 AIGRLVQEFEWKLRDGEEENVDTYGLTSQKLYPLMAIINPR 507

BLAST of CaUC05G099130 vs. TAIR 10
Match: AT2G40890.1 (cytochrome P450, family 98, subfamily A, polypeptide 3 )

HSP 1 Score: 152.1 bits (383), Expect = 1.2e-36
Identity = 117/471 (24.84%), Postives = 218/471 (46.28%), Query Frame = 0

Query: 81  PLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASISS 140
           P++GNL  +K  +    +  WA++YGP+ S+  G+   +V+++AE+AKE +      ++ 
Sbjct: 35  PIVGNLYDIKPVR-FRCYYEWAQSYGPIISVWIGSILNVVVSSAELAKEVLKEHDQKLAD 94

Query: 141 RKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRD----TMIEN 200
           R  +++    + +   +  +DY   +  V++     +      +  R  R+     M+E+
Sbjct: 95  RHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLELFTPKRLESLRPIREDEVTAMVES 154

Query: 201 ISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFRV 260
           + R+   +L +  ++ +  RK   A  F    +   GK     +++  G    +   F+ 
Sbjct: 155 VFRD--CNLPENRAKGLQLRKYLGAVAFNNITRLAFGKR----FMNAEGVVDEQGLEFKA 214

Query: 261 LVIDPMEGAIEVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMTMKSLLEEQ---KKRIAA 320
           +V + ++    +   +  P+LRW+   + E        RR    ++++EE    +++ + 
Sbjct: 215 IVSNGLKLGASLSIAEHIPWLRWM-FPADEKAFAEHGARRDRLTRAIMEEHTLARQKSSG 274

Query: 321 GEETNCYADFLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYLY 380
            ++    A   L +   L+ED I  LLW+ I    DTT +                  + 
Sbjct: 275 AKQHFVDALLTLKDQYDLSEDTIIGLLWDMITAGMDTTAITAEWAMAEMIKNPRVQQKVQ 334

Query: 381 QHILNVCGSAK-LTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPAG 440
           +    V G  + LTE + S+LPYL  V  E+ R H P P++    ++ D ++GGY +P G
Sbjct: 335 EEFDRVVGLDRILTEADFSRLPYLQCVVKESFRLHPPTPLMLPHRSNADVKIGGYDIPKG 394

Query: 441 SEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDLH--KTMAFGGGKRVCAGALKAM 500
           S + VN++A   D   W++P E+ PERFL+E  D M  H  + + FG G+RVC GA   +
Sbjct: 395 SNVHVNVWAVARDPAVWKNPFEFRPERFLEEDVD-MKGHDFRLLPFGAGRRVCPGAQLGI 454

Query: 501 LIACTTIGRMVQEFEWRLRDG----EEDKVDTLGLTARKLQPLHVVVKPRI 522
            +  + +  ++  F W    G    E D  +  GL      P+  V  PR+
Sbjct: 455 NLVTSMMSHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQAVATPRL 496

BLAST of CaUC05G099130 vs. TAIR 10
Match: AT1G74550.1 (cytochrome P450, family 98, subfamily A, polypeptide 9 )

HSP 1 Score: 147.9 bits (372), Expect = 2.3e-35
Identity = 115/471 (24.42%), Postives = 217/471 (46.07%), Query Frame = 0

Query: 78  PGVP---LLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTR 137
           PG P   L+GNL QLK      +F+ W++TYGP+ S+  G+   +V++++++AK+ +  +
Sbjct: 29  PGPPTRFLVGNLHQLKPLWT-QSFSEWSQTYGPIISVWLGSQLAVVVSSSDLAKQVLRDK 88

Query: 138 YASISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRDTMI 197
              + +R  +  +T   +D   +  SDY   +  +++     +    + +  R  R+  +
Sbjct: 89  DYQLCNRHRTARMTQNGSD---LIWSDYGAHYVKMRKLCTLELFSLKSIECFRSMREMEV 148

Query: 198 ENISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLF 257
            ++ + +F        + V  R   ++    +  +  +GK  E    DG      RE  F
Sbjct: 149 SSMVKSIFNDFMSDDQKPVVLRNYLDSVALNIVSRLVIGKTFEP--KDG------RE--F 208

Query: 258 RVLVIDPMEGAIEVDWRDFFPYLRWIPNKSVENR--IKNMDFRRRMTMKSLLEEQKKRIA 317
           R +V             D+  +L+ + +    ++  +K+M  +R    +++++E    + 
Sbjct: 209 RSIVERETRLPGATKMLDYTVWLKRLSSWFTSDKAFMKHMARKRNWFKRAVMDE----VY 268

Query: 318 AGEETNCYADFL--LSEAKTLTEDQISMLLWETIIETSDTTLV----------------D 377
            G +  C+   L  L E   LTE+ +  L+W  +   +DTT +                +
Sbjct: 269 GGRDQKCFVQSLLELKEKDELTEETVMGLVWNMLTAGADTTAITIEWAMAEMIRCPTVKE 328

Query: 378 YLYQHILNVCGSAKL-TEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFV 437
            +   + +V GS +L ++ ++ +LP+L  V  E LR H P P++    A E  Q+GGY V
Sbjct: 329 KVQDELDSVVGSGRLMSDADIPKLPFLQCVLKEALRLHPPTPLMLPHKASESVQVGGYKV 388

Query: 438 PAGSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDL-HKTMAFGGGKRVCAGALK 497
           P G+ + VN+ A   D  +W +P+E+ PERFL E+ D      + + FG G+RVC  A  
Sbjct: 389 PKGATVYVNVQAIARDPANWSNPDEFRPERFLVEETDVKGQDFRVLPFGSGRRVCPAAQL 448

Query: 498 AMLIACTTIGRMVQEFEWRLRDGEE--DKVDTLGLTARKLQPLHVVVKPRI 522
           ++ +    +G ++  F W      E  D  +  GL      PL  +   R+
Sbjct: 449 SLNMMTLALGSLLHCFSWTSSTPREHIDMTEKPGLVCYMKAPLQALASSRL 481

BLAST of CaUC05G099130 vs. TAIR 10
Match: AT4G13770.1 (cytochrome P450, family 83, subfamily A, polypeptide 1 )

HSP 1 Score: 142.9 bits (359), Expect = 7.3e-34
Identity = 117/475 (24.63%), Postives = 228/475 (48.00%), Query Frame = 0

Query: 80  VPLLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASIS 139
           +P++GNLLQL++  P   FA WA+ YGP+ S R G+ T++V+++AE+AKE + T+  + +
Sbjct: 37  LPVIGNLLQLQKLNPQRFFAGWAKKYGPILSYRIGSRTMVVISSAELAKELLKTQDVNFA 96

Query: 140 SRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGAN--AQKKHRQRRDT--MIE 199
            R   +    ++  +  +A++ Y  +++ +++  + ++      A  KH +  +   M++
Sbjct: 97  DRPPHRGHEFISYGRRDMALNHYTPYYREIRKMGMNHLFSPTRVATFKHVREEEARRMMD 156

Query: 200 NISRELFAHLKDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFR 259
            I++          SE V+  ++       +  ++  GK       DG       E++ R
Sbjct: 157 KINKAAD------KSEVVDISELMLTFTNSVVCRQAFGKKYNE---DG-------EEMKR 216

Query: 260 VLVIDPMEGAI--EVDWRDFFPYLRWIPNKSVENRIKNMDFRRRMT-MKSLLEEQKKRIA 319
            + I     ++  ++ + DFFPY  ++ + S         F R+ T ++ ++ E      
Sbjct: 217 FIKILYGTQSVLGKIFFSDFFPYCGFLDDLSGLTAYMKECFERQDTYIQEVVNETLDPKR 276

Query: 320 AGEETNCYADFLLS------EAKTLTEDQISMLLWETIIETSDTTL------VDYL--YQ 379
              ET    D L+        A   T D +  ++ + ++  +DT        + YL  Y 
Sbjct: 277 VKPETESMIDLLMGIYKEQPFASEFTVDNVKAVILDIVVAGTDTAAAAVVWGMTYLMKYP 336

Query: 380 HILNVC-----------GSAKLTEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQ 439
            +L              GS  +TE+++  LPY  A+  ETLR    +P++  R   +DT+
Sbjct: 337 QVLKKAQAEVREYMKEKGSTFVTEDDVKNLPYFRALVKETLRIEPVIPLLIPRACIQDTK 396

Query: 440 LGGYFVPAGSEIAVNIYACNMDKNHW-ESPEEWNPERFLDEKYDPMDL-HKTMAFGGGKR 499
           + GY +PAG+ + VN +A + D+  W  +P+E+ PERFL+++ D     ++ + FG G+R
Sbjct: 397 IAGYDIPAGTTVNVNAWAVSRDEKEWGPNPDEFRPERFLEKEVDFKGTDYEFIPFGSGRR 456

Query: 500 VCAGALKAMLIACTTIGRMVQEFEWRLRDG---EEDKVDTL-GLTARKLQPLHVV 517
           +C G      +       ++  F ++L +G   ++  +D + GL   K Q L +V
Sbjct: 457 MCPGMRLGAAMLEVPYANLLLSFNFKLPNGMKPDDINMDVMTGLAMHKSQHLKLV 495

BLAST of CaUC05G099130 vs. TAIR 10
Match: AT1G74540.1 (cytochrome P450, family 98, subfamily A, polypeptide 8 )

HSP 1 Score: 140.2 bits (352), Expect = 4.7e-33
Identity = 119/468 (25.43%), Postives = 216/468 (46.15%), Query Frame = 0

Query: 82  LLGNLLQLKEKKPHMTFARWAETYGPVYSIRTGASTVIVLNTAEVAKEAMVTRYASISSR 141
           LLGNL Q+K    H +F+ W+ETYGP+ S+  G+   +V++++++A++ +  +   +S+R
Sbjct: 36  LLGNLHQMKPLWTH-SFSEWSETYGPIISVWIGSQLTVVVSSSDLARQVLRDKDHQLSNR 95

Query: 142 KLSKALTILTADKCMVAMSDYNDFHKMVKRYILANVLGANAQKKHRQRRD----TMIENI 201
                +T    D   +  SDY+  +  +++     +    + +  R  R+    +M+ +I
Sbjct: 96  HRIARMTQTGTD---LVWSDYSPHYVKLRKLCTLELFSLKSIENFRSLREMEARSMVVSI 155

Query: 202 SRELFAHL-KDLPSETVNFRKIFEAELFRLALKETLGKDVESIYVDGLGTTLPREDLFRV 261
            ++L ++   D   + V  RK   A +     +  +GK+  S         + +E L   
Sbjct: 156 LKDLMSNSGDDQERKPVIVRKYLAAVVLNTISRLMIGKEFGSEEGKEFKAIVEKEHL--- 215

Query: 262 LVIDPMEGAIEVDWRDFFPYLRWIPNKSVENR--IKNMDFRRRMTMKSLLEEQKKRIAAG 321
                + G+  +   D   +L+W+ +    ++  + + D R +    +++ E+   I   
Sbjct: 216 -----LSGSGTI--LDHVWWLKWVSSWFFSDKEFLAHKDRRTKWFRGAIMVEEDIEI--- 275

Query: 322 EETNCYAD--FLLSEAKTLTEDQISMLLWETIIETSDTTLV----------------DYL 381
           E+   +     +L E K L+E+ +  L+W  +   +DTT V                +  
Sbjct: 276 EDHRGFVRKLLVLKEQKELSEETVGGLVWNMLTAGADTTAVVIEWAMAEMIKCPTVQEKA 335

Query: 382 YQHILNVCGSAKL-TEENLSQLPYLTAVFHETLRRHSPVPVVPLRYAHEDTQLGGYFVPA 441
            Q + +V GS +L TE ++  LPYL  V  E LR H   P++    A E   +GGY VP 
Sbjct: 336 QQELDSVVGSERLMTESDIPILPYLQCVVKEALRLHPSTPLMLPHKASETVWVGGYKVPK 395

Query: 442 GSEIAVNIYACNMDKNHWESPEEWNPERFLDEKYDPMDL-HKTMAFGGGKRVCAGALKAM 501
           G+ + VN+ A   D  +W +P E+ PERFL E+ D      + + FG G+R+C  A  +M
Sbjct: 396 GATVYVNVQAIGRDPANWINPYEFRPERFLQEETDVKGRDFRVLPFGSGRRMCPAAQLSM 455

Query: 502 LIACTTIGRMVQEFEWRLR-DGEE-DKVDTLGLTARKLQPLHVVVKPR 521
            +    +G ++  F W     GE  D  +  GL      PL  +  PR
Sbjct: 456 NLMTLVMGNLLHCFSWSSPVPGERIDMSENPGLLCNMRTPLQALALPR 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898390.11.1e-26887.73ent-kaurene oxidase [Benincasa hispida][more]
XP_008452488.16.4e-26686.62PREDICTED: ent-kaurene oxidase, chloroplastic [Cucumis melo] >KAA0064350.1 ent-k... [more]
NP_001267703.14.3e-26285.69ent-kaurene oxidase, chloroplastic-like [Cucumis sativus] >AFH03045.1 ent-kauren... [more]
KAG7024228.14.2e-24982.76Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022976063.17.9e-24880.48ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0G2RKY11.6e-17960.15Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1[more]
Q93ZB21.2e-16963.12Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV... [more]
Q5Z5R41.3e-15256.80Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ... [more]
Q5Z5R71.3e-14955.97Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19... [more]
Q0DBF44.5e-13751.62Ent-sandaracopimaradiene 3-hydroxylase OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Match NameE-valueIdentityDescription
A0A5D3D9I53.1e-26686.62Ent-kaurene oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G... [more]
A0A1S3BTZ03.1e-26686.62ent-kaurene oxidase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103493505 PE=3 ... [more]
J7I3T12.1e-26285.69Ent-kaurene oxidase OS=Cucumis sativus OX=3659 GN=ko PE=2 SV=1[more]
A0A6J1IL063.8e-24880.48ent-kaurene oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476... [more]
A0A6J1F7C81.0e-24579.93ent-kaurene oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT5G25900.18.3e-17163.12GA requiring 3 [more]
AT2G40890.11.2e-3624.84cytochrome P450, family 98, subfamily A, polypeptide 3 [more]
AT1G74550.12.3e-3524.42cytochrome P450, family 98, subfamily A, polypeptide 9 [more]
AT4G13770.17.3e-3424.63cytochrome P450, family 83, subfamily A, polypeptide 1 [more]
AT1G74540.14.7e-3325.43cytochrome P450, family 98, subfamily A, polypeptide 8 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 102..121
score: 34.81
coord: 381..399
score: 32.21
coord: 126..147
score: 21.43
coord: 422..446
score: 36.33
coord: 457..467
score: 51.84
coord: 467..490
score: 23.02
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 75..522
e-value: 2.0E-81
score: 275.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 78..520
IPR001128Cytochrome P450PFAMPF00067p450coord: 79..502
e-value: 2.2E-53
score: 181.7
IPR044225Ent-kaurene oxidase, chloroplasticPANTHERPTHR47283ENT-KAURENE OXIDASE, CHLOROPLASTICcoord: 24..520
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 460..469

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC05G099130.1CaUC05G099130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010241 ent-kaurene oxidation to kaurenoic acid
biological_process GO:0009686 gibberellin biosynthetic process
cellular_component GO:0009707 chloroplast outer membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0052615 ent-kaurene oxidase activity
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen