CaUC03G050970 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC03G050970
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionPhosphopyruvate hydratase
LocationCiama_Chr03: 1095530 .. 1101121 (-)
RNA-Seq ExpressionCaUC03G050970
SyntenyCaUC03G050970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGAAGCAGCTGAAAGAAATGTCCGTTCAAGAGTATCTGGACAAGTTCATGCTATCGCGGAAGATCGAGGAAGCGGTCAATGCTGCTGTTAGGGCGAAAACTCCCGATCCCGTTCTATTCATCGTAATCTCTCTCTCTCTCTCTCTCTCTCCGTTTTGGCTTATTAAGAAATGCTGGTGGAAATTTAATTTAATTGAATTGTTTTTCTTACAATCAATCATCGAAACAGGAGGAAATTGTATTCAATTGTTTTGTAGCGCAGTTCATTCTTCATCTTTGTTTACTTTTTTTTTTTTTTGAACAGTCGAATCATATGAAGAAAGCAATTCCTTCAGTGATTACAAAAATTAAAGCCCGGCAGATCCTTGATAGTAGAGGAATTCCGACTGTTGAAGTCGATTTATACACCAACAAAGGAGTGTTCCACGCTTCCGTGCCCAGCGGCGATCCGGCTGGCATGTAAGGTTCCATGGATATTAGCATGAGCTTCTATTTCAAAGAAAACAATTATCTTCATATGAAAAGCATTTGTTAGAGTCTTGCGACTGTTCTGGTCGCATTTTCAATTTCAACGCATGGCTTCCTTATACCTCTCGAGTTCCTATACATTTATTTTCAATTAGATTTTGAGAGTATAGTTGCATTTCCTAGGTATGAGGCTGTAGAGTTACGCGATGGAGACAAGGGAATATACCTTGGAAATAGTGTTACTAAAGCAGTCAAAAATATCAACGAGAAGATATCGGAAGCATTGATTGGCAGGGATCCGACGCAACAATATGAAATTGACCAGGCCATGAAAGATTTGGATACGACAGAAAAGAAGGTGGTAGTTCATCTATTTTCCTGATCTCACAAACTTGATGTGAGTGTTACTTATATGCCAACTTCTGCATCATTACTGTGATCTGCCATATCTGGTACTTGTGAGTATTAATGAAATGTGAATGGATTGAGAAGATTGAATTTAGTTAATATCCTATACTGCACAGTCTATTGGGAAATCGGACTAGTTATTTTTATGCTGAATTTTCTTATCATGTGCTACTACATCATAATTTACATGTTTTAGTAAATTTAAATTCCAGCCTGCCTTGAAGATTCTCTTAGCAAAAGTTTTGCTTATGCTGATACTGATTGGCTAAATTTGTTGCGACAATCAGTTCTGAGTTAAGGAGTGTGGCTCTGACATCTCAGATTCTCAACCCAATATGAAAGAAGAAGAACTCTGCATCTATACTGACTTAGAGTCACATGGATATGGATCAATGTTACTCTTTTTACTTGAAACAGGACATAACAAATTTTCCGTCATTTTTTTTCCTACTTCCTGTGCTATTTTGGACTTTTCATTATGATCATGATAATGTGTGTGTTTGTCTGTATAATAGATGGTTTATGATTAGAAGATAATCTCATTGGATTGTTCCTTCTTTCACAAAAAGAATTTATTTTCTAAGGTGGTCTATTGTGATGCCTTTTAATTTAGTAGTCTTCTTTGGCAATACATTCTGTAGGGTGAACTTGGAGCAAATGCTATACTTGCCGTGTCAATTGCTGTATGCAGAGCTGGTGCTGCTGAAAAGGAGGCATGCCTTTTATTGATCTATTTTATATGGCTTTAATTGTTATTGTTTTTCCCCCTTTTTTTACGGGTATTTGTACAATCCTGCATGAAGATTCTTAAACGAAGTAATTTCAGGTTCCACTCTATAAGCATATTGCAGACCTTGCTGGCAAAACAAACTTGATACTTCCTGTCCCAGCCTTCACTGTACTTAGTGGAGGAAAACATGCTGGCAATAATCTGGCCATTCAGGTATGCTGTGGTAAGGATCAAAGGAGTTGTGACCATTCTTTTACTTCTTCTTTTTCTGTTAGCTGTGAGGGTTCTTTAAACCGCATATAGGCTATAGTAAATAATCAGTTCAAGAAACCATCGACTCTGAATGTACTCTTTTTGTCTAGTAGTTCAGTCTATACACAATTATATATATGGAAGCCATCGTGGCTTGTAAGTATACTAGGTTATATTGATATTGAAGGACTCCCATTGTTGTGTACCGTGTCTATTGATTTAGACTGACTTGAATTGTTATCAGGAAATCATGATTCTTCCAATTGGAGCTAGCAAGTTTGAAGAGGCATTAAAAATGGGATCTGAAACTTTCCATCACTTAAAGGTGTGATTTCATTCATCAACTGTCCAATCTCTGGGTTTTTAACTTCACCTTCTCAGTGCATTCAATTATGTCAGTTCAAATATTTTGTAAAAGGAATATCTTCTAGTTCGTCTTCTTCTTTCTCTACACGATTATTTAATATTTTCATGCCATTCTATGATATTTATATGTATATCCATATTATCAAATAAGTATTGCTATTTTAATTTTACAATTTGTTGAGTTTTTTTTAGTATTACGCTTCATTGAAGGATCTAATTAGTATCAAAAGTATAAATTAAACCCTACATTTTCTTTGTATAGGATATCTCATGCATAGCACCAACTTTAGAGATTCATAGTCGTCCATGTACATTTGTGGAGCCTTAAAGCATTCTCTGTCTCATGGCATGGTCTATTTTAGTTTTCCTAGCTGTGTTAGGTTCCTTAACCATTATTGTAGCTTACCAAGTGTGTTCAAAATGCCAAAATTACAGACAAACATGTTGTAAAGTTGTGCATGGCTTAACCACTAATCAATCTATTTAAAAATAATTTCATTATATTTTCCTGGGGTTTTTGCTGAAATTCCAAGCAATTTAGTTCTCTATTTGGAAGCGGCATTGTTAATTATTTATGCCATATATTATTAAAAATGTAATTTCTTTGGAAAAAAAATGGCAGGCTGTAATTACTGAAAAGCATGGTGCCCAAGGATGTAATTTTGGTGAAGATGGTGGATTTGCCCCTAATATATCCAGGCAAGATACAAGTACCAATTTTTTTTTGGTTTGTTTATATGGATCTGTGGAACTAGTTTTTCCAAGCCACAATGAAGAACATTCTAGCATGAGAAAGATAGATTACATGTTTGACAGAACCCTGCATAGTGTGTACTGTGTACTTCTATAGTTGTTGGCAGCTTTGTTGAGTCATTGAGATAGCACTGAGCCAAAAACATGTTTTTAATTAGTTGTCTTCTATTGACAGTTTTAAAGAAGCCCTGGATCTTGTAGAAGAGGCTATTGGCAGAGCAGGGTATAACGAGAGAATCAAGATAGCAATAGATGTTGCTGCTACTAATTTCTGCATGGGTGAGACAGACAAGTTTCCTGAATAAGAGCCTAATTTGAACAATTGGATATGCGTACTACTTCTTGATGATAATAATCCACGTTCATCACCCTTTCTCCAATTATTTTCCTTACTGACAGCCTCCATAAATAAAACCCATTAGTTTGCTTACATGAAATGAATCTTTCCTGACCTGCACCAACATTCCCCTAAAGTTACATCTTCCGTTGGTAGGTACCAAATATGATCTTGACTTCAAAGCTCCAAACAAGTCGGTTCAAAATTTCAAGTCAGGAAAGGATATGATAGATATGTACAAAGAACTATGTGCTGGTATATATGTCTCTTCTCTCTTGTACAGCTTTATCATTATATATTTTTTCTGCTTGTTTTCAACAATGGGCTAATGTTCTCTGCTCTTTGTCTTTAGACTATCCAATTGTATCCATTGAAGATCCATTTGACCGGGAGGACTGGGATCACACCAAACATTTTTCTAGCCTTGGAATTTGTCAGGTTTCTCCTCATACTCATATCTGAAGTGTCTATCGGAAGAATATTTATTTCAACTCAAAGGACTGAAGATTGTGGTCGAAAATCTGAGGTCTAGTGCTGAGCATAATGTTAAAAGATATTTGTATGATCAAGACCCAAGAAGTTATGAGTTCAAATTCCCTGATACCACTTGTAAATTTATAATATCCGTATGCCTTTAGTTTCTTGCCTCGCCATGTATGTTACTAACTTACTATTGTGTCAGATTATTCTCCTTCATTTTGAGCCTGATGATCTCGTAGAACGTCAATTTGATTACCCTTTTTAACATCAAATTGCTAGCAAATTTGTAGAATACAGTTAACCAAACTGTCAAAGAATCAGACAACTTGGTTGTGTTTCCTCTTATAGTAGTTGAGGAGGCTTGTCGATATCTTTCTGACTAACAGAAAAAAAAAAGAAAAAAAGAAGAATAGGAAGTTAAAATCCTTTATATTATATATGAGTTAAATGTTTTCTTGAGCTTCTGAATTGGGAAGCTTTTGTTTCTGTTTCTGTTTAATTTTCTTATTATAAGTTCACTTAATCCCGTAGTGTTGTACTCTTTAACTGGTACCACTGCCCAATGCTGCTTCAATAATTTTGATTTTATGTAGGTAGTAGGAGGTGACTTGTTAATGTCAAACAAAAAGCGAATTGAGAGAGCAATTGATGAGTTCACTTGTAATGCCCTCCTCCTCAAGGTACAAACCACTTTTATTGTTATTATTATTATATTTACATTTACACATGTAACATTTCTTTGAGTATCTTCTTTTCGACATACTGTGGAATATTCTCATGTCTGAAGTCTGTTCATAATATCATTAATCCTACGAGGCTATGGAGAATTGGAACTAAAATACCATACAGACCTTTTGCAAGTGTATTTTTTGGGTGCAAAAAGCAACGTTCCACAGGGAAGTCTGATTTGACAGAAATCTGAAGAAGTGTTTACTTCCCATAGTGCAAGGGCATGATTTTAAAATGACTAAAATCAATTTTGTCATGTTTGAAATTACTCCGAAGCATGTCTTTAATCATTCAAAATTCGTTTATTGTTTAGTTTCACACTTTTAACCACAATTTTTATTTTTATAGGATCAAATTTGATCTTGAAGGATTAAAAACATGTTTTGAATATTTTCAAACATAACAAAAGTGATTTTGACCATTTCAAAATCACTCCCAAATATGGCCTATAATCTCACCAATTTGGCCTTTTCAATTTTGATAATTTCATGGGACAAAATGTAGGTAAACCAGATTGGTACAGTGACAGAAGCCATTGAAGTCGTGAAGCTTGCCAAGGATGCTCAATGGGCAGTTGTGGCATCTCACAGATGTGGAGAAACCGAGGATACATTCCTAGCCGATTTATCTGTTGGACTTTCCACATGTCAGATCAAAGCAGGTGCACCGTGTAGAGGAGAACGGCTAGCCAAATATAATCAGGTCTAATTTCTTGAACATTTGCCTCACTTGTCTAATTTTCTTCGTTTCGACATTACATGCACGCTGGTGAAGGGAATGGATGGCGTTAAATATAATACAACTCAGTTCGAGAATCTTCAAAAGACAATAATGAAAGCTTTTTGTATTGCATTGCCTTAGCTGATAATCAAAAGACAGTAGACTCTTATGGTGTCGTGTCGTCAATTTTGACATTTCTCAAAATGACAAGATTACAATTTCCTTAACTAGAATGAAGGCACGTTACTTATAGTCTCTGAGGTTAATGCAGTTGCTCCGTATTGAAGAAGAGCTTGGAGATCAAGCAATTTATGCTGGTGAAGATTGGAGGGCAGCTTGCTGA

mRNA sequence

ATGGAGAAGAAGCAGCTGAAAGAAATGTCCGTTCAAGAGTATCTGGACAAGTTCATGCTATCGCGGAAGATCGAGGAAGCGGTCAATGCTGCTGTTAGGGCGAAAACTCCCGATCCCGTTCTATTCATCTCGAATCATATGAAGAAAGCAATTCCTTCAGTGATTACAAAAATTAAAGCCCGGCAGATCCTTGATAGTAGAGGAATTCCGACTGTTGAAGTCGATTTATACACCAACAAAGGAGTGTTCCACGCTTCCGTGCCCAGCGGCGATCCGGCTGGCATGTATGAGGCTGTAGAGTTACGCGATGGAGACAAGGGAATATACCTTGGAAATAGTGTTACTAAAGCAGTCAAAAATATCAACGAGAAGATATCGGAAGCATTGATTGGCAGGGATCCGACGCAACAATATGAAATTGACCAGGCCATGAAAGATTTGGATACGACAGAAAAGAAGGGTGAACTTGGAGCAAATGCTATACTTGCCGTGTCAATTGCTGTATGCAGAGCTGGTGCTGCTGAAAAGGAGGTTCCACTCTATAAGCATATTGCAGACCTTGCTGGCAAAACAAACTTGATACTTCCTGTCCCAGCCTTCACTGTACTTAGTGGAGGAAAACATGCTGGCAATAATCTGGCCATTCAGGAAATCATGATTCTTCCAATTGGAGCTAGCAAGTTTGAAGAGGCATTAAAAATGGGATCTGAAACTTTCCATCACTTAAAGGCTGTAATTACTGAAAAGCATGGTGCCCAAGGATGTAATTTTGGTGAAGATGGTGGATTTGCCCCTAATATATCCAGTTTTAAAGAAGCCCTGGATCTTGTAGAAGAGGCTATTGGCAGAGCAGGGTATAACGAGAGAATCAAGATAGCAATAGATGTTGCTGCTACTAATTTCTGCATGGGTACCAAATATGATCTTGACTTCAAAGCTCCAAACAAGTCGGTTCAAAATTTCAAGTCAGGAAAGGATATGATAGATATGTACAAAGAACTATGTGCTGACTATCCAATTGTATCCATTGAAGATCCATTTGACCGGGAGGACTGGGATCACACCAAACATTTTTCTAGCCTTGGAATTTGTCAGGTAGTAGGAGGTGACTTGTTAATGTCAAACAAAAAGCGAATTGAGAGAGCAATTGATGAGTTCACTTGTAATGCCCTCCTCCTCAAGGTAAACCAGATTGGTACAGTGACAGAAGCCATTGAAGTCGTGAAGCTTGCCAAGGATGCTCAATGGGCAGTTGTGGCATCTCACAGATGTGGAGAAACCGAGGATACATTCCTAGCCGATTTATCTGTTGGACTTTCCACATGTCAGATCAAAGCAGGTGCACCGTGTAGAGGAGAACGGCTAGCCAAATATAATCAGTTGCTCCGTATTGAAGAAGAGCTTGGAGATCAAGCAATTTATGCTGGTGAAGATTGGAGGGCAGCTTGCTGA

Coding sequence (CDS)

ATGGAGAAGAAGCAGCTGAAAGAAATGTCCGTTCAAGAGTATCTGGACAAGTTCATGCTATCGCGGAAGATCGAGGAAGCGGTCAATGCTGCTGTTAGGGCGAAAACTCCCGATCCCGTTCTATTCATCTCGAATCATATGAAGAAAGCAATTCCTTCAGTGATTACAAAAATTAAAGCCCGGCAGATCCTTGATAGTAGAGGAATTCCGACTGTTGAAGTCGATTTATACACCAACAAAGGAGTGTTCCACGCTTCCGTGCCCAGCGGCGATCCGGCTGGCATGTATGAGGCTGTAGAGTTACGCGATGGAGACAAGGGAATATACCTTGGAAATAGTGTTACTAAAGCAGTCAAAAATATCAACGAGAAGATATCGGAAGCATTGATTGGCAGGGATCCGACGCAACAATATGAAATTGACCAGGCCATGAAAGATTTGGATACGACAGAAAAGAAGGGTGAACTTGGAGCAAATGCTATACTTGCCGTGTCAATTGCTGTATGCAGAGCTGGTGCTGCTGAAAAGGAGGTTCCACTCTATAAGCATATTGCAGACCTTGCTGGCAAAACAAACTTGATACTTCCTGTCCCAGCCTTCACTGTACTTAGTGGAGGAAAACATGCTGGCAATAATCTGGCCATTCAGGAAATCATGATTCTTCCAATTGGAGCTAGCAAGTTTGAAGAGGCATTAAAAATGGGATCTGAAACTTTCCATCACTTAAAGGCTGTAATTACTGAAAAGCATGGTGCCCAAGGATGTAATTTTGGTGAAGATGGTGGATTTGCCCCTAATATATCCAGTTTTAAAGAAGCCCTGGATCTTGTAGAAGAGGCTATTGGCAGAGCAGGGTATAACGAGAGAATCAAGATAGCAATAGATGTTGCTGCTACTAATTTCTGCATGGGTACCAAATATGATCTTGACTTCAAAGCTCCAAACAAGTCGGTTCAAAATTTCAAGTCAGGAAAGGATATGATAGATATGTACAAAGAACTATGTGCTGACTATCCAATTGTATCCATTGAAGATCCATTTGACCGGGAGGACTGGGATCACACCAAACATTTTTCTAGCCTTGGAATTTGTCAGGTAGTAGGAGGTGACTTGTTAATGTCAAACAAAAAGCGAATTGAGAGAGCAATTGATGAGTTCACTTGTAATGCCCTCCTCCTCAAGGTAAACCAGATTGGTACAGTGACAGAAGCCATTGAAGTCGTGAAGCTTGCCAAGGATGCTCAATGGGCAGTTGTGGCATCTCACAGATGTGGAGAAACCGAGGATACATTCCTAGCCGATTTATCTGTTGGACTTTCCACATGTCAGATCAAAGCAGGTGCACCGTGTAGAGGAGAACGGCTAGCCAAATATAATCAGTTGCTCCGTATTGAAGAAGAGCTTGGAGATCAAGCAATTTATGCTGGTGAAGATTGGAGGGCAGCTTGCTGA

Protein sequence

MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWRAAC
Homology
BLAST of CaUC03G050970 vs. NCBI nr
Match: XP_004145264.1 (cytosolic enolase 3 [Cucumis sativus] >KGN65781.1 hypothetical protein Csa_023204 [Cucumis sativus])

HSP 1 Score: 951.4 bits (2458), Expect = 2.9e-273
Identity = 477/483 (98.76%), Postives = 479/483 (99.17%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVITEKHGA GCNFGEDGGFAPNISSFKEALDLVEEAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 481 AAC 484
           AAC
Sbjct: 481 AAC 483

BLAST of CaUC03G050970 vs. NCBI nr
Match: XP_038893436.1 (cytosolic enolase 3 [Benincasa hispida])

HSP 1 Score: 949.1 bits (2452), Expect = 1.5e-272
Identity = 475/483 (98.34%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGA KFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGACKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLK VITEKHGA GCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN
Sbjct: 241 HLKVVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKR+ERAIDEFTCNALLLKVNQIGTVTEAIEVVK+AKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRMERAIDEFTCNALLLKVNQIGTVTEAIEVVKVAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQ IYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQGIYAGEDWR 480

Query: 481 AAC 484
           AAC
Sbjct: 481 AAC 483

BLAST of CaUC03G050970 vs. NCBI nr
Match: XP_008457408.1 (PREDICTED: cytosolic enolase 3 [Cucumis melo])

HSP 1 Score: 948.7 bits (2451), Expect = 1.9e-272
Identity = 476/483 (98.55%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVITEKHGA  CNFGEDGGFAPNISSFKEALDLVEEAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 481 AAC 484
           AAC
Sbjct: 481 AAC 483

BLAST of CaUC03G050970 vs. NCBI nr
Match: TYJ97346.1 (cytosolic enolase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 938.3 bits (2424), Expect = 2.6e-269
Identity = 476/499 (95.39%), Postives = 478/499 (95.79%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFI----------------S 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFI                S
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFIVISPFPSLCVVFLGNVS 60

Query: 61  NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 120
           NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG
Sbjct: 61  NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 120

Query: 121 DKGIYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 180
           DKG YLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV
Sbjct: 121 DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 180

Query: 181 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 240
           SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG
Sbjct: 181 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 240

Query: 241 ASKFEEALKMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRA 300
           ASKFEEALKMGSETFHHLKAVITEKHGA  CNFGEDGGFAPNISSFKEALDLVEEAI R 
Sbjct: 241 ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG 300

Query: 301 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 360
           GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE
Sbjct: 301 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 360

Query: 361 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 420
           DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA
Sbjct: 361 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 420

Query: 421 IEVVKLAKDAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 480
           IEVVKLAKDAQWAVVASHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI
Sbjct: 421 IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 480

Query: 481 EEELGDQAIYAGEDWRAAC 484
           EEELGDQAIYAGEDWRAAC
Sbjct: 481 EEELGDQAIYAGEDWRAAC 499

BLAST of CaUC03G050970 vs. NCBI nr
Match: XP_022949451.1 (cytosolic enolase 3 [Cucurbita moschata] >XP_023525808.1 cytosolic enolase 3 [Cucurbita pepo subsp. pepo] >KAG7036966.1 Cytosolic enolase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 917.9 bits (2371), Expect = 3.6e-263
Identity = 458/480 (95.42%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVI+EKHGA GCNFGEDGGFAPNISSF EALDLV+EAIGR GYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

BLAST of CaUC03G050970 vs. ExPASy Swiss-Prot
Match: Q9ZW34 (Cytosolic enolase 3 OS=Arabidopsis thaliana OX=3702 GN=ENO3 PE=1 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 2.8e-226
Identity = 384/472 (81.36%), Postives = 429/472 (90.89%), Query Frame = 0

Query: 9   MSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKARQILDSRG 68
           MSVQEYLDK MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKA+ SVITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKNINEKISEA 128
           IPTVEVDL+TNKGVF ASVPSGD +G YEA+ELRDGDKG+YLGNSV KAVKNINEKISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           LIG DP  Q +IDQAM DLD TEKK ELGANAILAVSIA C+AGAAEKEVPL KH++DL+
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 189 GKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITE 248
           G+ N++LPVPAFTVLSGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVI+E
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 249 KHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATNFCMGTKYD 308
           K+G  GCN GEDGG AP+ISS KE L+LV+EAI R GYN++IKIAID+AATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 309 LDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSSLGICQVVG 368
           LD K+PNKS QNFKS +DMIDMYKE+C DYPIVSIEDPFD+EDW+HTK+FSSLGICQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 369 GDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVASHRCGETE 428
            DLLMSN KR+ERAI E +CNALLLKVNQIGTVTEAIEVVK+A+DAQW VV SHRCGETE
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 429 DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 481
           D+F++DLSVGL+T  IKAGAPCRGER  KYNQLLRIEEELGDQA+YAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of CaUC03G050970 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 510.0 bits (1312), Expect = 3.0e-143
Identity = 256/428 (59.81%), Postives = 317/428 (74.07%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSV 114
           ITKI ARQI DSRG PTVEVDL+T KGVF A+VPSG   G++EA+ELRDGDK ++LG  V
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 115 TKAVKNINEKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
            KAV NINEKI+ ALI  G D T Q +ID  M  LD +E KG LGANAIL VS+AV +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A  K +PLYK+IA+LAG   ++LPVPAF V++GG HAGN LA+QE MILP+GAS F EA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKI 294
           +MGSE +HHLKA I +++G      G++GGFAPNI   KE LDL+  AI +AGY  +I I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK P      + SG+ + ++Y+    +YP+VSIED FD++DW
Sbjct: 243 GMDVAASEFFKDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDDW 302

Query: 355 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 414
           D+   F      Q+VG DL ++N KRI+ AID+ +CN LLLKVNQIG+VTE+IE  KL++
Sbjct: 303 DNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLSR 362

Query: 415 DAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              W V+ SHR GETEDTF+ADL VGL+T QIK GAPCR ERLAKYNQLLRIEEELG  A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGADA 422

Query: 475 IYAGEDWR 481
           +YAG ++R
Sbjct: 423 VYAGHNFR 430

BLAST of CaUC03G050970 vs. ExPASy Swiss-Prot
Match: P09104 (Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3)

HSP 1 Score: 508.1 bits (1307), Expect = 1.1e-142
Identity = 255/428 (59.58%), Postives = 322/428 (75.23%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSV 114
           I KI AR+ILDSRG PTVEVDLYT KG+F A+VPSG   G+YEA+ELRDGDK  YLG  V
Sbjct: 3   IEKIWAREILDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDGDKQRYLGKGV 62

Query: 115 TKAVKNINEKISEALI--GRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
            KAV +IN  I+ ALI  G    +Q ++D  M +LD TE K + GANAIL VS+AVC+AG
Sbjct: 63  LKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           AAE+E+PLY+HIA LAG ++LILPVPAF V++GG HAGN LA+QE MILP+GA  F +A+
Sbjct: 123 AAERELPLYRHIAQLAGNSDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGAESFRDAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKI 294
           ++G+E +H LK VI +K+G    N G++GGFAPNI    EAL+LV+EAI +AGY E+I I
Sbjct: 183 RLGAEVYHTLKGVIKDKYGKDATNVGDEGGFAPNILENSEALELVKEAIDKAGYTEKIVI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK+P      + +G  +  +Y++   DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSPT-DPSRYITGDQLGALYQDFVRDYPVVSIEDPFDQDDW 302

Query: 355 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 414
                F++    Q+VG DL ++N KRIERA++E  CN LLLKVNQIG+VTEAI+  KLA+
Sbjct: 303 AAWSKFTANVGIQIVGDDLTVTNPKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQ 362

Query: 415 DAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
           +  W V+ SHR GETEDTF+ADL VGL T QIK GAPCR ERLAKYNQL+RIEEELGD+A
Sbjct: 363 ENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEA 422

Query: 475 IYAGEDWR 481
            +AG ++R
Sbjct: 423 RFAGHNFR 429

BLAST of CaUC03G050970 vs. ExPASy Swiss-Prot
Match: P51913 (Alpha-enolase OS=Gallus gallus OX=9031 GN=ENO1 PE=2 SV=2)

HSP 1 Score: 504.6 bits (1298), Expect = 1.2e-141
Identity = 251/428 (58.64%), Postives = 326/428 (76.17%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSV 114
           I KI AR+I DSRG PTVEVDLYTNKG+F A+VPSG   G+YEA+ELRD DK  YLG  V
Sbjct: 3   ILKIHAREIFDSRGNPTVEVDLYTNKGLFRAAVPSGASTGIYEALELRDNDKTRYLGKGV 62

Query: 115 TKAVKNINEKISEALIGR--DPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
           +KAV+++N+ I+ ALI +  +  +Q +ID+ M ++D TE K + GANAIL VS+AVC+AG
Sbjct: 63  SKAVEHVNKTIAPALISKNVNVVEQEKIDKLMLEMDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           AAEK VPLY+HIADLAG   +ILPVPAF V++GG HAGN LA+QE MILP+GA  F+EA+
Sbjct: 123 AAEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGADTFKEAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKI 294
           ++G+E +H+LK VI EK+G    N G++GGFAPNI   KEAL+L++ AIG+AGY++++ I
Sbjct: 183 RIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIGKAGYSDKVVI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK+P+     + S   + D+Y     +YP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFYRDGKYDLDFKSPD-DPSRYISPDQLADLYLGFVKNYPVVSIEDPFDQDDW 302

Query: 355 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 414
              K F++    QVVG DL ++N KRI +A++E +CN LLLKVNQIG+VTE+++  KLA+
Sbjct: 303 AAWKKFTASVGIQVVGDDLTVTNPKRIAKAVEEKSCNCLLLKVNQIGSVTESLQACKLAQ 362

Query: 415 DAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              W V+ SHR GETEDTF+ADL VGL T QIK GAPCR ERLAKYNQLLRIEEELG +A
Sbjct: 363 SNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKA 422

Query: 475 IYAGEDWR 481
            +AG ++R
Sbjct: 423 RFAGRNFR 429

BLAST of CaUC03G050970 vs. ExPASy Swiss-Prot
Match: P06733 (Alpha-enolase OS=Homo sapiens OX=9606 GN=ENO1 PE=1 SV=2)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 248/428 (57.94%), Postives = 326/428 (76.17%), Query Frame = 0

Query: 55  ITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSV 114
           I KI AR+I DSRG PTVEVDL+T+KG+F A+VPSG   G+YEA+ELRD DK  Y+G  V
Sbjct: 3   ILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGV 62

Query: 115 TKAVKNINEKISEALIGR--DPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAG 174
           +KAV++IN+ I+ AL+ +  + T+Q +ID+ M ++D TE K + GANAIL VS+AVC+AG
Sbjct: 63  SKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A EK VPLY+HIADLAG + +ILPVPAF V++GG HAGN LA+QE MILP+GA+ F EA+
Sbjct: 123 AVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKI 294
           ++G+E +H+LK VI EK+G    N G++GGFAPNI   KE L+L++ AIG+AGY +++ I
Sbjct: 183 RIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK+P+     + S   + D+YK    DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFFRSGKYDLDFKSPD-DPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDW 302

Query: 355 DHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAK 414
              + F++    QVVG DL ++N KRI +A++E +CN LLLKVNQIG+VTE+++  KLA+
Sbjct: 303 GAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQ 362

Query: 415 DAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              W V+ SHR GETEDTF+ADL VGL T QIK GAPCR ERLAKYNQLLRIEEELG +A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKA 422

Query: 475 IYAGEDWR 481
            +AG ++R
Sbjct: 423 KFAGRNFR 429

BLAST of CaUC03G050970 vs. ExPASy TrEMBL
Match: A0A0A0M0P5 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G528460 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.4e-273
Identity = 477/483 (98.76%), Postives = 479/483 (99.17%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVITEKHGA GCNFGEDGGFAPNISSFKEALDLVEEAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 481 AAC 484
           AAC
Sbjct: 481 AAC 483

BLAST of CaUC03G050970 vs. ExPASy TrEMBL
Match: A0A1S3C645 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103497104 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 9.2e-273
Identity = 476/483 (98.55%), Postives = 478/483 (98.96%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVITEKHGA  CNFGEDGGFAPNISSFKEALDLVEEAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

Query: 481 AAC 484
           AAC
Sbjct: 481 AAC 483

BLAST of CaUC03G050970 vs. ExPASy TrEMBL
Match: A0A5D3BEA1 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001560 PE=3 SV=1)

HSP 1 Score: 938.3 bits (2424), Expect = 1.2e-269
Identity = 476/499 (95.39%), Postives = 478/499 (95.79%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFI----------------S 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFI                S
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFIVISPFPSLCVVFLGNVS 60

Query: 61  NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 120
           NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG
Sbjct: 61  NHMKKAIPSVITKIKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDG 120

Query: 121 DKGIYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 180
           DKG YLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV
Sbjct: 121 DKGTYLGNSVTKAVKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAV 180

Query: 181 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 240
           SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG
Sbjct: 181 SIAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIG 240

Query: 241 ASKFEEALKMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRA 300
           ASKFEEALKMGSETFHHLKAVITEKHGA  CNFGEDGGFAPNISSFKEALDLVEEAI R 
Sbjct: 241 ASKFEEALKMGSETFHHLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRG 300

Query: 301 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 360
           GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE
Sbjct: 301 GYNERIKIAIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 360

Query: 361 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 420
           DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA
Sbjct: 361 DPFDREDWDHTKHFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEA 420

Query: 421 IEVVKLAKDAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 480
           IEVVKLAKDAQWAVVASHRCGET+DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI
Sbjct: 421 IEVVKLAKDAQWAVVASHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRI 480

Query: 481 EEELGDQAIYAGEDWRAAC 484
           EEELGDQAIYAGEDWRAAC
Sbjct: 481 EEELGDQAIYAGEDWRAAC 499

BLAST of CaUC03G050970 vs. ExPASy TrEMBL
Match: A0A6J1GC24 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111452790 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.7e-263
Identity = 458/480 (95.42%), Postives = 469/480 (97.71%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVI+EKHGA GCNFGEDGGFAPNISSF EALDLV+EAIGR GYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

BLAST of CaUC03G050970 vs. ExPASy TrEMBL
Match: A0A6J1KFE0 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111493370 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.2e-261
Identity = 455/481 (94.59%), Postives = 470/481 (97.71%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60
           MEKKQLK+MSVQEYLDK+MLSRKIEEAVNAAVRAKTPDPVLFISNHMKKA+PS+ITKIKA
Sbjct: 1   MEKKQLKQMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDL+TNKGVF+ASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLHTNKGVFNASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180
           IN+KISEALIGRDPTQQYEIDQAMK+LDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKNLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLAGKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300
           HLKAVI+EKHGA GCNFGEDGGFAPNISSF EALDLV+EAIGR GYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTKHFS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420
           LGICQVVGGDLLMSNKKRIERAIDE TCNALLLKVNQIGTVTEAIEVV LAKDAQWAVVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480
           SHRCGET+DTFLADLSVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 A 482
           A
Sbjct: 481 A 481

BLAST of CaUC03G050970 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 785.8 bits (2028), Expect = 2.0e-227
Identity = 384/472 (81.36%), Postives = 429/472 (90.89%), Query Frame = 0

Query: 9   MSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKARQILDSRG 68
           MSVQEYLDK MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKA+ SVITK+KARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKAVKNINEKISEA 128
           IPTVEVDL+TNKGVF ASVPSGD +G YEA+ELRDGDKG+YLGNSV KAVKNINEKISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPLYKHIADLA 188
           LIG DP  Q +IDQAM DLD TEKK ELGANAILAVSIA C+AGAAEKEVPL KH++DL+
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 189 GKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITE 248
           G+ N++LPVPAFTVLSGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVI+E
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 249 KHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATNFCMGTKYD 308
           K+G  GCN GEDGG AP+ISS KE L+LV+EAI R GYN++IKIAID+AATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 309 LDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSSLGICQVVG 368
           LD K+PNKS QNFKS +DMIDMYKE+C DYPIVSIEDPFD+EDW+HTK+FSSLGICQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 369 GDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVASHRCGETE 428
            DLLMSN KR+ERAI E +CNALLLKVNQIGTVTEAIEVVK+A+DAQW VV SHRCGETE
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 429 DTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 481
           D+F++DLSVGL+T  IKAGAPCRGER  KYNQLLRIEEELGDQA+YAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of CaUC03G050970 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 498.0 bits (1281), Expect = 8.3e-141
Identity = 260/438 (59.36%), Postives = 327/438 (74.66%), Query Frame = 0

Query: 53  SVITKIKARQILDSRGIPTVEVDLYTNKGV-FHASVPSGDPAGMYEAVELRDGDKGIYLG 112
           + IT +KARQI DSRG PTVEVD++T+ G+   A+VPSG   G+YEA+ELRDG    YLG
Sbjct: 2   ATITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSD-YLG 61

Query: 113 NSVTKAVKNINEKISEALIGRDPTQQYEIDQAM-KDLDTTEK-----KGELGANAILAVS 172
             V+KAV N+N  I  ALIG+DPTQQ  ID  M  +LD T+      K +LGANAILAVS
Sbjct: 62  KGVSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVS 121

Query: 173 IAVCRAGAAEKEVPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGA 232
           +AVC+AGA    +PLYKHIA+LAG   ++LPVPAF V++GG HAGN LA+QE MILP+GA
Sbjct: 122 LAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGA 181

Query: 233 SKFEEALKMGSETFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAG 292
           + F+EA+KMG E +HHLK+VI +K+G    N G++GGFAPNI   KE L+L++ AI +AG
Sbjct: 182 ASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAG 241

Query: 293 YNERIKIAIDVAATNFCMGTK-YDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIE 352
           Y  ++ I +DVAA+ F    K YDL+FK  N +     SG  + D+YK   A+YPIVSIE
Sbjct: 242 YTGKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIE 301

Query: 353 DPFDREDWDH-TKHFSSLGI-CQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVT 412
           DPFD++DW+H  K  +  G   Q+VG DLL++N KR+ +AI E +CNALLLKVNQIG+VT
Sbjct: 302 DPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVT 361

Query: 413 EAIEVVKLAKDAQWAVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLL 472
           E+IE VK++K A W V+ SHR GETEDTF+ADL+VGLST QIK GAPCR ERLAKYNQLL
Sbjct: 362 ESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLL 421

Query: 473 RIEEELGDQAIYAGEDWR 481
           RIEEELG +AIYAG ++R
Sbjct: 422 RIEEELGSEAIYAGVNFR 438

BLAST of CaUC03G050970 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 473.4 bits (1217), Expect = 2.2e-133
Identity = 239/425 (56.24%), Postives = 309/425 (72.71%), Query Frame = 0

Query: 58  IKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGIYLGNSVTKA 117
           +KARQI+DSRG PTVEVDL T+  ++ ++VPSG   G+YEA+ELRDGDK +Y G  V +A
Sbjct: 54  VKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQA 113

Query: 118 VKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKE 177
           +KNINE ++  LIG D   Q ++D  M +LD T  K +LGANAIL VS++VCRAGA  K 
Sbjct: 114 IKNINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKG 173

Query: 178 VPLYKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSE 237
           VPLYKHI + +G   L++PVPAF V++GG HAGN+LA+QE MILP+GA+ F EA +MGSE
Sbjct: 174 VPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSE 233

Query: 238 TFHHLKAVITEKHGAQGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVA 297
            +H LK +I  K+G   CN G++GGFAPN+   +E L L+ +AI +AGY  +IKI +DVA
Sbjct: 234 VYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVA 293

Query: 298 ATNFCM-GTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK 357
           A+ F M   +YDL+FK       +  S + + D+Y+E   D+PIVSIEDPFD++DW    
Sbjct: 294 ASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWA 353

Query: 358 HFSSLGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQW 417
              S    Q+VG DLL++N KRI  AI + +CNALLLKVNQIGTVTE+I+    +K A W
Sbjct: 354 SLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGW 413

Query: 418 AVVASHRCGETEDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAG 477
            V+ SHR GETED F+ADLSVGL++ QIK GAPCR ERL+KYNQLLRIEEELG+   YAG
Sbjct: 414 GVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGN-VRYAG 473

Query: 478 EDWRA 482
           E +R+
Sbjct: 474 EAFRS 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004145264.12.9e-27398.76cytosolic enolase 3 [Cucumis sativus] >KGN65781.1 hypothetical protein Csa_02320... [more]
XP_038893436.11.5e-27298.34cytosolic enolase 3 [Benincasa hispida][more]
XP_008457408.11.9e-27298.55PREDICTED: cytosolic enolase 3 [Cucumis melo][more]
TYJ97346.12.6e-26995.39cytosolic enolase 3 [Cucumis melo var. makuwa][more]
XP_022949451.13.6e-26395.42cytosolic enolase 3 [Cucurbita moschata] >XP_023525808.1 cytosolic enolase 3 [Cu... [more]
Match NameE-valueIdentityDescription
Q9ZW342.8e-22681.36Cytosolic enolase 3 OS=Arabidopsis thaliana OX=3702 GN=ENO3 PE=1 SV=1[more]
Q275273.0e-14359.81Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3[more]
P091041.1e-14259.58Gamma-enolase OS=Homo sapiens OX=9606 GN=ENO2 PE=1 SV=3[more]
P519131.2e-14158.64Alpha-enolase OS=Gallus gallus OX=9031 GN=ENO1 PE=2 SV=2[more]
P067331.6e-14157.94Alpha-enolase OS=Homo sapiens OX=9606 GN=ENO1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0M0P51.4e-27398.76Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G528460 PE=3 SV=1[more]
A0A1S3C6459.2e-27398.55Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103497104 PE=3 SV=1[more]
A0A5D3BEA11.2e-26995.39Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1GC241.7e-26395.42Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111452790 PE=3 SV=... [more]
A0A6J1KFE01.2e-26194.59Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111493370 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G29560.12.0e-22781.36cytosolic enolase [more]
AT2G36530.18.3e-14159.36Enolase [more]
AT1G74030.12.2e-13356.24enolase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 214..227
score: 59.31
coord: 391..405
score: 76.87
coord: 87..101
score: 62.42
coord: 420..437
score: 73.06
coord: 368..379
score: 48.11
coord: 157..173
score: 75.58
IPR000941EnolaseTIGRFAMTIGR01060TIGR01060coord: 55..478
e-value: 1.4E-149
score: 496.4
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 55..477
IPR000941EnolaseHAMAPMF_00318Enolasecoord: 53..468
score: 39.467815
IPR000941EnolaseCDDcd03313enolasecoord: 57..467
e-value: 0.0
score: 643.766
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 55..184
e-value: 2.2E-77
score: 273.1
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 55..184
e-value: 2.6E-53
score: 179.7
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 192..482
e-value: 8.8E-163
score: 556.7
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 193..480
e-value: 7.3E-121
score: 403.0
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 49..177
e-value: 3.5E-55
score: 187.8
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 55..188
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 178..483
e-value: 5.6E-141
score: 471.0
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 193..480
NoneNo IPR availableSFLDSFLDS00001Enolasecoord: 54..449
e-value: 0.0
score: 170.0
NoneNo IPR availablePANTHERPTHR11902:SF1ENOLASEcoord: 55..477
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 391..404

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC03G050970.1CaUC03G050970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity