CaUC02G040850 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC02G040850
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	GDSL esterase/lipase
Location	Ciama_Chr02: 27962621 .. 27964250 (-)
RNA-Seq Expression	CaUC02G040850
Synteny	CaUC02G040850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCACGAGCTAAGAATTACCTCATTGCAGCTCTATCATTCCACATCATTTGGCTGCTATTTCTTAGCAAACCAGGCAGTGCTTTAAAGCCTAAAATTCCTCGGTCGTTTCCCGCCATTCTCATATTTGGTGATTCTACGGTGGATACTGGAAATAACAACTTCATTTCCACCATTTTCAAGGCCAATTACTTTCCATATGGCAAAAATTTTCCTGGCCATGTTAGTACTGGAAGGTTCAGTGATGGAAAACTCATTCCTGATATGGTGGCCTCTAGGTTAGGGATAAAGGAGTTTGTTCCTCCATTTTTGGATCCTAAACTGTCGAATGATGATGTGAAAACGGGGGTTAGTTTTGCATCGGCTGGCACCGGCTTTGATAATCTGACAGCCATTATATCTAGAGTCATTCCTGTGATGAAGCAAATCAATCTTTTCAAGAACTACACTGAAAGGCTTCAAAAGATTGTAGGTGCAAGTCAAAGTAGGAGGATTCTTAAGAATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTCAACGTCAACTTTTATGACATTCCCACAAGGAAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAGCAGGCTACAAACCTTGATCAAGGTACATTATACAATAGGCACATTATAATTAAATTCTAAAAACAAAAACACGTTTTCAGAACTATATCTTTTTAGCTTTCAAAACTTGACTTGGATTCTAAAAACATCTCGAAAAAGTAGATATCGCAACAAAGGAACACATGAGTAAGATTAGGGTTCATAAGTAATTTGCAAAATCCAAAAACTACAAACCAAATAGATATCAAACAGAGTCTAAATTATAACTTTTTATATCTAGTTTTGTATTCAATCAATTCTTGTACTTTAAAAAAAAAATTACTGGGCGTCAAATTTTAGTCCTACGTCTAAACTAAAATTTACTTGAAAAACATTCTTAGTTTATACTTGTATCACCAGTACAAACACTTGAACTACTGAAAATAAACACTTATAACTCTGTTTTTAATTGAAAATGGACAGAAAATTTACCGGCTTGGATGTCGCAATATGGTGGTCGCTGGCCTCCCTCCAATTGGTTGTCTTCCCATTCAACAGACTATGTCGATTCGGAATCCCTTCAATCGAAGTTGTATCAAAGAACAAAACTCAGATTCAAAAGCCTATAATCAGAAACTTTCAAAGCTACTAAAGAATTTACAGCGAAAGCTTCATGGAAGCAAAATTTTTTATGCTGACATCTACACCCCCATTAACGACATGCTCAATAATCCTAAGAAATATGGTAAGTCTGAGTCGGTGGTACTTAAAATTGAATCTAATGCAGTAAACATGTAAGGGGAAATGCAGTTTTGAGAGCCATTCTGAACTAAAATTTTATTGGGGCATTTCGTCGAACAGGTTTTGAGCGTACCGATAAAGGTTGCTGTGGAAGTGGGTTGAATGAAGCAGGGCCTCTGTGTAATTCAATAACTCCAACATGTAAAAACCCATCAAAATTTATGTTCTGGGATAGCATTCATCCCACTGAAGCAGCCTACAAGATCATCGCGGATTCGCTTTTAAAGAAACTTGACGATTTACTGTATCGGAATTAA

mRNA sequence

ATGGCACGAGCTAAGAATTACCTCATTGCAGCTCTATCATTCCACATCATTTGGCTGCTATTTCTTAGCAAACCAGGCAGTGCTTTAAAGCCTAAAATTCCTCGGTCGTTTCCCGCCATTCTCATATTTGGTGATTCTACGGTGGATACTGGAAATAACAACTTCATTTCCACCATTTTCAAGGCCAATTACTTTCCATATGGCAAAAATTTTCCTGGCCATGTTAGTACTGGAAGGTTCAGTGATGGAAAACTCATTCCTGATATGGTGGCCTCTAGGTTAGGGATAAAGGAGTTTGTTCCTCCATTTTTGGATCCTAAACTGTCGAATGATGATGTGAAAACGGGGGTTAGTTTTGCATCGGCTGGCACCGGCTTTGATAATCTGACAGCCATTATATCTAGAGTCATTCCTGTGATGAAGCAAATCAATCTTTTCAAGAACTACACTGAAAGGCTTCAAAAGATTGTAGGTGCAAGTCAAAGTAGGAGGATTCTTAAGAATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTCAACGTCAACTTTTATGACATTCCCACAAGGAAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAGCAGGCTACAAACCTTGATCAAGAAAATTTACCGGCTTGGATGTCGCAATATGGTGGTCGCTGGCCTCCCTCCAATTGGTTGTCTTCCCATTCAACAGACTATGTCGATTCGGAATCCCTTCAATCGAAGTTGTATCAAAGAACAAAACTCAGATTCAAAAGCCTATAATCAGAAACTTTCAAAGCTACTAAAGAATTTACAGCGAAAGCTTCATGGAAGCAAAATTTTTTATGCTGACATCTACACCCCCATTAACGACATGCTCAATAATCCTAAGAAATATGGTTTTGAGCGTACCGATAAAGGTTGCTGTGGAAGTGGGTTGAATGAAGCAGGGCCTCTGTGTAATTCAATAACTCCAACATGTAAAAACCCATCAAAATTTATGTTCTGGGATAGCATTCATCCCACTGAAGCAGCCTACAAGATCATCGCGGATTCGCTTTTAAAGAAACTTGACGATTTACTGTATCGGAATTAA

Coding sequence (CDS)

ATGGCACGAGCTAAGAATTACCTCATTGCAGCTCTATCATTCCACATCATTTGGCTGCTATTTCTTAGCAAACCAGGCAGTGCTTTAAAGCCTAAAATTCCTCGGTCGTTTCCCGCCATTCTCATATTTGGTGATTCTACGGTGGATACTGGAAATAACAACTTCATTTCCACCATTTTCAAGGCCAATTACTTTCCATATGGCAAAAATTTTCCTGGCCATGTTAGTACTGGAAGGTTCAGTGATGGAAAACTCATTCCTGATATGGTGGCCTCTAGGTTAGGGATAAAGGAGTTTGTTCCTCCATTTTTGGATCCTAAACTGTCGAATGATGATGTGAAAACGGGGGTTAGTTTTGCATCGGCTGGCACCGGCTTTGATAATCTGACAGCCATTATATCTAGAGTCATTCCTGTGATGAAGCAAATCAATCTTTTCAAGAACTACACTGAAAGGCTTCAAAAGATTGTAGGTGCAAGTCAAAGTAGGAGGATTCTTAAGAATGCTTTTGTTGTGATTAGTGCAGGAACCAATGACTTCAACGTCAACTTTTATGACATTCCCACAAGGAAGCTACAGTACAATATTAGTGGTTACCAAGATTTTATACAGAGCAGGCTACAAACCTTGATCAAGAAAATTTACCGGCTTGGATGTCGCAATATGGTGGTCGCTGGCCTCCCTCCAATTGGTTGTCTTCCCATTCAACAGACTATGTCGATTCGGAATCCCTTCAATCGAAGTTGTATCAAAGAACAAAACTCAGATTCAAAAGCCTATAATCAGAAACTTTCAAAGCTACTAAAGAATTTACAGCGAAAGCTTCATGGAAGCAAAATTTTTTATGCTGACATCTACACCCCCATTAACGACATGCTCAATAATCCTAAGAAATATGGTTTTGAGCGTACCGATAAAGGTTGCTGTGGAAGTGGGTTGAATGAAGCAGGGCCTCTGTGTAATTCAATAACTCCAACATGTAAAAACCCATCAAAATTTATGTTCTGGGATAGCATTCATCCCACTGAAGCAGCCTACAAGATCATCGCGGATTCGCTTTTAAAGAAACTTGACGATTTACTGTATCGGAATTAA

Protein sequence

MARAKNYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDDLLYRN

Homology

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BLAST of CaUC02G040850 vs. NCBI nr
Match: XP_004151059.1 (GDSL esterase/lipase At2g30310 [Cucumis sativus] >KGN63338.1 hypothetical protein Csa_022358 [Cucumis sativus])

HSP 1 Score: 577.8 bits (1488), Expect = 6.6e-161
Identity = 279/360 (77.50%), Postives = 319/360 (88.61%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLL-FLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTI 60
           MAR  N LIAALS H IWLL  L+KP S+L+PK   SFPAILIFGDSTVDTGNNNFI TI
Sbjct: 1   MARV-NCLIAALSLHTIWLLVVLTKPCSSLEPKTTPSFPAILIFGDSTVDTGNNNFIPTI 60

Query: 61  FKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSF 120
           FK NY PYGKNFPGH++TGRFSDGKLIPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSF
Sbjct: 61  FKGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSF 120

Query: 121 ASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTND 180
           ASAGTGFD+LTA IS+VIPVMKQI+ FKNY +RLQ +VG  +S+RI+ NA VVISAGTND
Sbjct: 121 ASAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGVVGVDESKRIINNALVVISAGTND 180

Query: 181 FNVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTM 240
            N+NFYD+PTR+LQYNISGYQDF+Q+RLQ+LIK+IY+LGCRN+VVAGLPP+GCLPIQ+T+
Sbjct: 181 LNINFYDLPTRQLQYNISGYQDFLQNRLQSLIKEIYQLGCRNIVVAGLPPVGCLPIQETI 240

Query: 241 SIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKY 300
           +  NP  R+C+K+QNSDS AYNQKLSKLL NLQ +L GSKI YADIYTP+ DMLNNP+KY
Sbjct: 241 AFENPLKRNCLKDQNSDSVAYNQKLSKLLTNLQPQLAGSKILYADIYTPLIDMLNNPQKY 300

Query: 301 GFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDD 360
           GF+ T++GCCG+GL EAGPLCN  TPTC+N SKFMFWDSIHPTEAAYK IA++LLKKL D
Sbjct: 301 GFDHTNRGCCGTGLVEAGPLCNPKTPTCENSSKFMFWDSIHPTEAAYKFIAEALLKKLGD 359

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BLAST of CaUC02G040850 vs. NCBI nr
Match: XP_016902930.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0066338.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK00940.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 575.9 bits (1483), Expect = 2.5e-160
Identity = 274/354 (77.40%), Postives = 312/354 (88.14%), Query Frame = 0


Query: 6   NYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIFKANYF 65
           N LIA LS H IWLL L+KP S+L+PK   SFPAILIFGDSTVDTGNNNFI TIFK NY 
Sbjct: 5   NCLIATLSLHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIFKGNYS 64

Query: 66  PYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTG 125
           PYGKNFPGH++TGRFSDGKLIPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSFASAGTG
Sbjct: 65  PYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG 124

Query: 126 FDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDFNVNFY 185
           FD+LTA IS+VIPVMKQI+ FKNY +RLQ +VG  +S+RI+ NA VVISAGTND N+NFY
Sbjct: 125 FDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDLNINFY 184

Query: 186 DIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMSIRNPF 245
           D+PTR+LQYNISGYQDF+Q+RL++LIK+IY+LGCRN+VVAGLPP+GCLPIQ+T++  NP 
Sbjct: 185 DLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPL 244

Query: 246 NRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYGFERTD 305
            R C+++QNSDS AYNQKLSKLL NLQ +L GSKI YADIYTP+ DMLNNP+ YGFE T+
Sbjct: 245 KRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKILYADIYTPLIDMLNNPQNYGFEHTN 304

Query: 306 KGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDD 360
           KGCCG+GL EAGPLCN   PTC+N SKFMFWDSIHPTEAAYK IA+SLLKKL D
Sbjct: 305 KGCCGTGLVEAGPLCNPKIPTCENSSKFMFWDSIHPTEAAYKFIAESLLKKLGD 358

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BLAST of CaUC02G040850 vs. NCBI nr
Match: XP_022947820.1 (GDSL esterase/lipase At2g30310-like [Cucurbita moschata])

HSP 1 Score: 563.9 bits (1452), Expect = 9.9e-157
Identity = 268/356 (75.28%), Postives = 316/356 (88.76%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIF 60
           MAR  NYLIAALS +IIW L + K GS    KI RSFPAILIFGDSTVDTGNNNFISTIF
Sbjct: 1   MARV-NYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY PYGK+FPGH++TGRFS+GKLIPDMVASRLGIKE VPPFLDPKLS DDVKTGVSFA
Sbjct: 61  KANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFA 120

Query: 121 SAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDF 180
           SAGTGFD+LTA IS+VIPVMKQI+LFKNYT+RLQ+I G  +S++I+ +A V+ISAGTND 
Sbjct: 121 SAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDL 180

Query: 181 NVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMS 240
           N+NFYDIPTRKLQYNISGYQDFI +RLQ+ IK+IY+LGCRN+VVAGLPP+GCLPIQ+T+S
Sbjct: 181 NINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVS 240

Query: 241 IRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYG 300
             NP +R C+++QNSDSK+YNQKLSKLL NLQ +L GS + YADIYTP+ DM+NNP +YG
Sbjct: 241 FENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYG 300

Query: 301 FERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKK 357
           FE T++GCCG+GL EAGPLCN++TPTC++PSK++FWDSIHPTE+ YK I +SLLK+
Sbjct: 301 FEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQ 355

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BLAST of CaUC02G040850 vs. NCBI nr
Match: XP_023533087.1 (GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563.1 bits (1450), Expect = 1.7e-156
Identity = 270/356 (75.84%), Postives = 317/356 (89.04%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIF 60
           MAR  NYLI ALS HII  L +SK  SA   KI RSFPAILIFGDSTVDTGNNNFISTIF
Sbjct: 1   MARV-NYLIGALSLHIILSLCISKLCSASDLKISRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY PYGK+FPGH++TGRFS+GKLIPDMVASRLGIKE VPPFLDPKLSNDDVKTGVSFA
Sbjct: 61  KANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSNDDVKTGVSFA 120

Query: 121 SAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDF 180
           SAGTGFD+LTA IS+VIPVMKQI+LFKNYT+RLQ+IVG  +S++I+ +A V+ISAGTND 
Sbjct: 121 SAGTGFDDLTATISKVIPVMKQIDLFKNYTKRLQRIVGVDESKKIIGSALVIISAGTNDL 180

Query: 181 NVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMS 240
           N+NFYDIPTRKLQYNISGYQDFI +RLQ+ IK+IY+LGCRN+VVAGLPP+GCLPIQ+T+S
Sbjct: 181 NINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVS 240

Query: 241 IRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYG 300
             NP +R C+++QNSDSK+YNQKLSKLL NLQ +L GS + YADIYTP+ DM+NNP++YG
Sbjct: 241 FENPLHRICLEDQNSDSKSYNQKLSKLLSNLQPQLPGSTMLYADIYTPLIDMINNPRQYG 300

Query: 301 FERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKK 357
           FE T+ GCCG+GL EAGPLCN++TPTC++PSK++FWDSIHPTE+ YK I +SLLK+
Sbjct: 301 FEHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQ 355

[Close]

BLAST of CaUC02G040850 vs. NCBI nr
Match: XP_023006812.1 (GDSL esterase/lipase At2g30310-like [Cucurbita maxima])

HSP 1 Score: 561.6 bits (1446), Expect = 4.9e-156
Identity = 267/356 (75.00%), Postives = 316/356 (88.76%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIF 60
           MARA NY IAALS HIIW L ++K   A   KI R+FPAILIFGDSTVDTGNNNFI TIF
Sbjct: 1   MARA-NYFIAALSLHIIWSLCVTKLCRASDLKISRTFPAILIFGDSTVDTGNNNFIPTIF 60

Query: 61  KANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY PYGK+FPGH++TGRFS+GKLIPDMVASRLGIKEFVPPFLDPKL NDDVKTGVSFA
Sbjct: 61  KANYAPYGKDFPGHIATGRFSNGKLIPDMVASRLGIKEFVPPFLDPKLLNDDVKTGVSFA 120

Query: 121 SAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDF 180
           SAGTGFD+LTA IS+VIPVMKQI+LFKNY +RLQ+IVG  +S++I+ +A V+ISAGTND 
Sbjct: 121 SAGTGFDDLTATISKVIPVMKQIDLFKNYIQRLQRIVGVDESKKIIGSALVIISAGTNDL 180

Query: 181 NVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMS 240
           N+NFYD+PTRKLQYNISGYQDFI +RLQ+ IK+IY+LGCRN+VVAGLPPIGCLPIQ+T+S
Sbjct: 181 NINFYDLPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPIGCLPIQETIS 240

Query: 241 IRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYG 300
            +NP NR C+++QNSDSK+YNQKLSKLL NLQ +L  SK+ YADIYTP+ DM+NNP +YG
Sbjct: 241 FQNPLNRICLEDQNSDSKSYNQKLSKLLSNLQPQLPRSKMLYADIYTPLMDMINNPHQYG 300

Query: 301 FERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKK 357
           FE T+ GCCG+GL EAGPLCN++TPTC++PSK++FWDSIHPTE+ YK I +SL+K+
Sbjct: 301 FEHTNTGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLIKQ 355

[Close]

BLAST of CaUC02G040850 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 7.0e-105
Identity = 182/317 (57.41%), Postives = 235/317 (74.13%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNFIS-TIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDSTVDTGNNN+ S TIFKA + PYG + PGH + GR+S+GK+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKE VPPFL P +S+ D+ TGVSFASAG G+D+ +++ S+ IPV +Q ++FKNY  RL+ 
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQY-NISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NA VVISAG NDF +NFYDIPTR+L+Y  I GYQ+FI  RL   ++++
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LGCRN+VV GLPP+GCLPIQ T  +RN   R C++++N DS  YNQKL K L  +Q  
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRN-ILRFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GS   YA++Y P+ DM+ NP KYGF+ T KGCCG+G  E   +CN +T TC N S  +
Sbjct: 272 LPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHL 331

Query: 337 FWDSIHPTEAAYKIIAD 352
           FWDSIHP+EAAY  I +
Sbjct: 332 FWDSIHPSEAAYNYIGN 347

[Close]

BLAST of CaUC02G040850 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.9e-104
Identity = 179/327 (54.74%), Postives = 238/327 (72.78%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNFIS-TIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDST DTGNNN+ S  +FKAN+ PYG + PGH + GRFS+GKLI D+++++L 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKEFVPPFL P +S+ D+ TGV FASAG G+D+ T++ S+ IPV +Q ++FKNY  RL+ 
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQY-NISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NA VVISAG NDF +NFYDIP R+L+Y  I GYQDF+  RL   ++++
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LGCRN++V GLPP+GCLPIQ T  +R      C++++N DS  YNQKL K L  +Q  
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQKLVKKLPEIQAS 270

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GSK  YA++Y P+ DM+ NP KYGF+ T KGCCG+G  E   LC S++ TC N S  +
Sbjct: 271 LPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSLSKTCPNHSDHL 330

Query: 337 FWDSIHPTEAAYKIIADSLLKKLDDLL 362
           FWDSIHP+EAAYK + + +  ++ + L
Sbjct: 331 FWDSIHPSEAAYKYLGNFIDAQIQEWL 356

[Close]

BLAST of CaUC02G040850 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 379.0 bits (972), Expect = 5.9e-104
Identity = 177/319 (55.49%), Postives = 238/319 (74.61%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNF-ISTIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDSTVDTGNNN+ + TIF+A +FPYG + P   + GRFS+GKLI D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKEF+PPFL P LS+ D+ TGV FASAG G+D+LT++ ++ I V +Q N+FK+Y  RL+ 
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQYN-ISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NAFVV+SAG NDF +N+YDIP+R+L+Y  ISGYQDFI  RL+  ++++
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYDIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LG RN++V GLPP+GCLPI  T   RN F R C++  N DS  YN+KL KLL  ++  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIF-RFCLEHHNKDSVLYNEKLQKLLPQIEAS 272

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GSK  YAD+Y P+ +M+ NP KYGF+ T +GCCG+G  E   +CN  +P C+N S+FM
Sbjct: 273 LPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFM 332

Query: 337 FWDSIHPTEAAYKIIADSL 354
           F+DSIHP+EA Y +I + L
Sbjct: 333 FFDSIHPSEATYNVIGNRL 350

[Close]

BLAST of CaUC02G040850 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-103
Identity = 170/325 (52.31%), Postives = 239/325 (73.54%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNFISTIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGI 96
           FPAIL+FGDST+DTGNNN+I T  +AN+ PYG NFPGH +TGRFS+GKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 97  KEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKI 156
           K+ VPPFLDP LS+ D+ TGV FASAG+G+DNLT   +  + V KQ ++ ++Y ERL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 157 VGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYR 216
           VG  ++  I+  A V++S+GTNDFN+N YD P+R+ +  + GYQ FI S +   ++++Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 217 LGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLH 276
           +GCR ++V GLPP+GCLPIQ TM+++    R CI +QNSDS+ +NQKL   L  +Q  L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 277 GSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFW 336
           GS IFY DIY  + DM  NP++YG + T +GCCG+G  E   LCN++T  C NP++++FW
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFW 334

Query: 337 DSIHPTEAAYKIIADSLLKKLDDLL 362
           D IHP++ AY +I+ SL++++  +L
Sbjct: 335 DDIHPSQIAYIVISLSLVEQIFHVL 359

[Close]

BLAST of CaUC02G040850 vs. ExPASy Swiss-Prot
Match: Q9SIQ3 (GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 1.9e-102
Identity = 174/319 (54.55%), Postives = 237/319 (74.29%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNF-ISTIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDSTVDTGNNN+ + TIF+A +FPYG + P   + GRFS+GKLI D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKEF+PPFL P LS+ D+ TGV FASAG G+D+LT++ ++ I V +Q N+FK+Y  RL+ 
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQYN-ISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NAFVV+SAG NDF +N+Y+IP+R+L+Y  ISGYQDFI  RL+  ++++
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LG RN++V GLPP+GCLPI  T   RN F R C++  N DS  YN+KL  LL  ++  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIF-RFCLEHHNKDSVLYNEKLQNLLPQIEAS 272

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GSK  YAD+Y P+ +M+ NP KYGF+ T +GCCG+G  E   +CN  +P C+N S+F+
Sbjct: 273 LPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFL 332

Query: 337 FWDSIHPTEAAYKIIADSL 354
           F+DSIHP+EA Y +I + L
Sbjct: 333 FFDSIHPSEATYNVIGNLL 350

[Close]

BLAST of CaUC02G040850 vs. ExPASy TrEMBL
Match: A0A0A0LNE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431080 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.2e-161
Identity = 279/360 (77.50%), Postives = 319/360 (88.61%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLL-FLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTI 60
           MAR  N LIAALS H IWLL  L+KP S+L+PK   SFPAILIFGDSTVDTGNNNFI TI
Sbjct: 1   MARV-NCLIAALSLHTIWLLVVLTKPCSSLEPKTTPSFPAILIFGDSTVDTGNNNFIPTI 60

Query: 61  FKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSF 120
           FK NY PYGKNFPGH++TGRFSDGKLIPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSF
Sbjct: 61  FKGNYSPYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSF 120

Query: 121 ASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTND 180
           ASAGTGFD+LTA IS+VIPVMKQI+ FKNY +RLQ +VG  +S+RI+ NA VVISAGTND
Sbjct: 121 ASAGTGFDDLTAAISKVIPVMKQIDHFKNYIQRLQGVVGVDESKRIINNALVVISAGTND 180

Query: 181 FNVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTM 240
            N+NFYD+PTR+LQYNISGYQDF+Q+RLQ+LIK+IY+LGCRN+VVAGLPP+GCLPIQ+T+
Sbjct: 181 LNINFYDLPTRQLQYNISGYQDFLQNRLQSLIKEIYQLGCRNIVVAGLPPVGCLPIQETI 240

Query: 241 SIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKY 300
           +  NP  R+C+K+QNSDS AYNQKLSKLL NLQ +L GSKI YADIYTP+ DMLNNP+KY
Sbjct: 241 AFENPLKRNCLKDQNSDSVAYNQKLSKLLTNLQPQLAGSKILYADIYTPLIDMLNNPQKY 300

Query: 301 GFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDD 360
           GF+ T++GCCG+GL EAGPLCN  TPTC+N SKFMFWDSIHPTEAAYK IA++LLKKL D
Sbjct: 301 GFDHTNRGCCGTGLVEAGPLCNPKTPTCENSSKFMFWDSIHPTEAAYKFIAEALLKKLGD 359

[Close]

BLAST of CaUC02G040850 vs. ExPASy TrEMBL
Match: A0A5A7VF47 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001150 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.2e-160
Identity = 274/354 (77.40%), Postives = 312/354 (88.14%), Query Frame = 0


Query: 6   NYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIFKANYF 65
           N LIA LS H IWLL L+KP S+L+PK   SFPAILIFGDSTVDTGNNNFI TIFK NY 
Sbjct: 5   NCLIATLSLHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIFKGNYS 64

Query: 66  PYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTG 125
           PYGKNFPGH++TGRFSDGKLIPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSFASAGTG
Sbjct: 65  PYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG 124

Query: 126 FDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDFNVNFY 185
           FD+LTA IS+VIPVMKQI+ FKNY +RLQ +VG  +S+RI+ NA VVISAGTND N+NFY
Sbjct: 125 FDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDLNINFY 184

Query: 186 DIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMSIRNPF 245
           D+PTR+LQYNISGYQDF+Q+RL++LIK+IY+LGCRN+VVAGLPP+GCLPIQ+T++  NP 
Sbjct: 185 DLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPL 244

Query: 246 NRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYGFERTD 305
            R C+++QNSDS AYNQKLSKLL NLQ +L GSKI YADIYTP+ DMLNNP+ YGFE T+
Sbjct: 245 KRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKILYADIYTPLIDMLNNPQNYGFEHTN 304

Query: 306 KGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDD 360
           KGCCG+GL EAGPLCN   PTC+N SKFMFWDSIHPTEAAYK IA+SLLKKL D
Sbjct: 305 KGCCGTGLVEAGPLCNPKIPTCENSSKFMFWDSIHPTEAAYKFIAESLLKKLGD 358

[Close]

BLAST of CaUC02G040850 vs. ExPASy TrEMBL
Match: A0A1S4E4N0 (GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501132 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.2e-160
Identity = 274/354 (77.40%), Postives = 312/354 (88.14%), Query Frame = 0


Query: 6   NYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIFKANYF 65
           N LIA LS H IWLL L+KP S+L+PK   SFPAILIFGDSTVDTGNNNFI TIFK NY 
Sbjct: 5   NCLIATLSLHTIWLLLLTKPCSSLEPKTSSSFPAILIFGDSTVDTGNNNFIPTIFKGNYS 64

Query: 66  PYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTG 125
           PYGKNFPGH++TGRFSDGKLIPDMVASRLGIKE VPPFLDPKLSNDD+KTGVSFASAGTG
Sbjct: 65  PYGKNFPGHLATGRFSDGKLIPDMVASRLGIKELVPPFLDPKLSNDDIKTGVSFASAGTG 124

Query: 126 FDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDFNVNFY 185
           FD+LTA IS+VIPVMKQI+ FKNY +RLQ +VG  +S+RI+ NA VVISAGTND N+NFY
Sbjct: 125 FDDLTAAISKVIPVMKQIDHFKNYIQRLQGLVGVGESKRIISNALVVISAGTNDLNINFY 184

Query: 186 DIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMSIRNPF 245
           D+PTR+LQYNISGYQDF+Q+RL++LIK+IY+LGCRN+VVAGLPP+GCLPIQ+T++  NP 
Sbjct: 185 DLPTRQLQYNISGYQDFLQNRLKSLIKEIYQLGCRNIVVAGLPPVGCLPIQETIAFENPL 244

Query: 246 NRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYGFERTD 305
            R C+++QNSDS AYNQKLSKLL NLQ +L GSKI YADIYTP+ DMLNNP+ YGFE T+
Sbjct: 245 KRHCLEDQNSDSIAYNQKLSKLLTNLQPQLSGSKILYADIYTPLIDMLNNPQNYGFEHTN 304

Query: 306 KGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDD 360
           KGCCG+GL EAGPLCN   PTC+N SKFMFWDSIHPTEAAYK IA+SLLKKL D
Sbjct: 305 KGCCGTGLVEAGPLCNPKIPTCENSSKFMFWDSIHPTEAAYKFIAESLLKKLGD 358

[Close]

BLAST of CaUC02G040850 vs. ExPASy TrEMBL
Match: A0A6J1G7Z0 (GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC111451580 PE=3 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.8e-157
Identity = 268/356 (75.28%), Postives = 316/356 (88.76%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIF 60
           MAR  NYLIAALS +IIW L + K GS    KI RSFPAILIFGDSTVDTGNNNFISTIF
Sbjct: 1   MARV-NYLIAALSLYIIWSLCIIKLGSTSDLKISRSFPAILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY PYGK+FPGH++TGRFS+GKLIPDMVASRLGIKE VPPFLDPKLS DDVKTGVSFA
Sbjct: 61  KANYSPYGKDFPGHMATGRFSNGKLIPDMVASRLGIKELVPPFLDPKLSKDDVKTGVSFA 120

Query: 121 SAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDF 180
           SAGTGFD+LTA IS+VIPVMKQI+LFKNYT+RLQ+I G  +S++I+ +A V+ISAGTND 
Sbjct: 121 SAGTGFDDLTATISKVIPVMKQIDLFKNYTQRLQRIAGVDESKKIIGSALVIISAGTNDL 180

Query: 181 NVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMS 240
           N+NFYDIPTRKLQYNISGYQDFI +RLQ+ IK+IY+LGCRN+VVAGLPP+GCLPIQ+T+S
Sbjct: 181 NINFYDIPTRKLQYNISGYQDFILNRLQSFIKEIYQLGCRNIVVAGLPPVGCLPIQETVS 240

Query: 241 IRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYG 300
             NP +R C+++QNSDSK+YNQKLSKLL NLQ +L GS + YADIYTP+ DM+NNP +YG
Sbjct: 241 FENPLHRICLEDQNSDSKSYNQKLSKLLSNLQSQLPGSTMLYADIYTPLIDMINNPHQYG 300

Query: 301 FERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSLLKK 357
           FE T++GCCG+GL EAGPLCN++TPTC++PSK++FWDSIHPTE+ YK I +SLLK+
Sbjct: 301 FEHTNRGCCGTGLAEAGPLCNALTPTCEDPSKYIFWDSIHPTESVYKSITESLLKQ 355

[Close]

BLAST of CaUC02G040850 vs. ExPASy TrEMBL
Match: A0A5A7UVN0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00270 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.4e-156
Identity = 272/362 (75.14%), Postives = 315/362 (87.02%), Query Frame = 0


Query: 1   MARAKNYLIAALSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFISTIF 60
           MARA  Y IAALS H+IWLLFLSKP +AL+PKI RSFP+ILIFGDSTVDTGNNNFISTIF
Sbjct: 1   MARA-TYHIAALSLHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIF 60

Query: 61  KANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFA 120
           KANY PYG +FPGHV+TGRFSDGKLIPDMVAS+LGIKE VPPFLD KLS+D+VKTGVSFA
Sbjct: 61  KANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFA 120

Query: 121 SAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDF 180
           SAG+GFD LTA +S VIPVMKQI++FKNY  RLQ IVG  +SR+IL +A V+ISAGTND 
Sbjct: 121 SAGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDV 180

Query: 181 NVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMS 240
           N+NFYD+PTR+LQYNISGYQDF+Q RLQ+LIK+IY+LGCR MVVAGLPP+GCLPIQ++++
Sbjct: 181 NINFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIA 240

Query: 241 IRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYG 300
            +NP +R C++EQNSDSKAYNQKL+ LL NLQ +L GS I YADIYTP+ DM+NNP  YG
Sbjct: 241 FQNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTILYADIYTPLIDMVNNPHNYG 300

Query: 301 FERTDKGCCGSGLNEAGPLCNSITPT-CKNPSKFMFWDSIHPTEAAYKIIADSLLKKLDD 360
           FE  + GCCG+G+ EAGPLCNS T T C+NPSKFMFWDSIHPTEAAYK I +SLLK+  D
Sbjct: 301 FEHVNVGCCGTGMAEAGPLCNSKTSTICENPSKFMFWDSIHPTEAAYKFITESLLKQFAD 360

Query: 361 LL 362
            L
Sbjct: 361 RL 361

[Close]

BLAST of CaUC02G040850 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 382.1 bits (980), Expect = 5.0e-106
Identity = 182/317 (57.41%), Postives = 235/317 (74.13%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNFIS-TIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDSTVDTGNNN+ S TIFKA + PYG + PGH + GR+S+GK+I D++AS+L 
Sbjct: 32  FPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKVISDVIASKLN 91

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKE VPPFL P +S+ D+ TGVSFASAG G+D+ +++ S+ IPV +Q ++FKNY  RL+ 
Sbjct: 92  IKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSMFKNYIARLKG 151

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQY-NISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NA VVISAG NDF +NFYDIPTR+L+Y  I GYQ+FI  RL   ++++
Sbjct: 152 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFILKRLDGFVREL 211

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LGCRN+VV GLPP+GCLPIQ T  +RN   R C++++N DS  YNQKL K L  +Q  
Sbjct: 212 YSLGCRNIVVGGLPPMGCLPIQMTAKMRN-ILRFCVEQENKDSVLYNQKLVKKLPEIQAS 271

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GS   YA++Y P+ DM+ NP KYGF+ T KGCCG+G  E   +CN +T TC N S  +
Sbjct: 272 LPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLTKTCPNHSDHL 331

Query: 337 FWDSIHPTEAAYKIIAD 352
           FWDSIHP+EAAY  I +
Sbjct: 332 FWDSIHPSEAAYNYIGN 347

[Close]

BLAST of CaUC02G040850 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.2e-105
Identity = 179/327 (54.74%), Postives = 238/327 (72.78%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNFIS-TIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDST DTGNNN+ S  +FKAN+ PYG + PGH + GRFS+GKLI D+++++L 
Sbjct: 31  FPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGKLISDVISTKLN 90

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKEFVPPFL P +S+ D+ TGV FASAG G+D+ T++ S+ IPV +Q ++FKNY  RL+ 
Sbjct: 91  IKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPSMFKNYIARLKG 150

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQY-NISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NA VVISAG NDF +NFYDIP R+L+Y  I GYQDF+  RL   ++++
Sbjct: 151 IVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFVLKRLDGFVREL 210

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LGCRN++V GLPP+GCLPIQ T  +R      C++++N DS  YNQKL K L  +Q  
Sbjct: 211 YSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQKLVKKLPEIQAS 270

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GSK  YA++Y P+ DM+ NP KYGF+ T KGCCG+G  E   LC S++ TC N S  +
Sbjct: 271 LPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSLSKTCPNHSDHL 330

Query: 337 FWDSIHPTEAAYKIIADSLLKKLDDLL 362
           FWDSIHP+EAAYK + + +  ++ + L
Sbjct: 331 FWDSIHPSEAAYKYLGNFIDAQIQEWL 356

[Close]

BLAST of CaUC02G040850 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 377.9 bits (969), Expect = 9.4e-105
Identity = 170/325 (52.31%), Postives = 239/325 (73.54%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNFISTIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLGI 96
           FPAIL+FGDST+DTGNNN+I T  +AN+ PYG NFPGH +TGRFS+GKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 97  KEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQKI 156
           K+ VPPFLDP LS+ D+ TGV FASAG+G+DNLT   +  + V KQ ++ ++Y ERL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 157 VGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQYNISGYQDFIQSRLQTLIKKIYR 216
           VG  ++  I+  A V++S+GTNDFN+N YD P+R+ +  + GYQ FI S +   ++++Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 217 LGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRKLH 276
           +GCR ++V GLPP+GCLPIQ TM+++    R CI +QNSDS+ +NQKL   L  +Q  L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 277 GSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFMFW 336
           GS IFY DIY  + DM  NP++YG + T +GCCG+G  E   LCN++T  C NP++++FW
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFW 334

Query: 337 DSIHPTEAAYKIIADSLLKKLDDLL 362
           D IHP++ AY +I+ SL++++  +L
Sbjct: 335 DDIHPSQIAYIVISLSLVEQIFHVL 359

[Close]

BLAST of CaUC02G040850 vs. TAIR 10
Match: AT2G31540.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 374.0 bits (959), Expect = 1.4e-103
Identity = 174/319 (54.55%), Postives = 237/319 (74.29%), Query Frame = 0


Query: 37  FPAILIFGDSTVDTGNNNF-ISTIFKANYFPYGKNFPGHVSTGRFSDGKLIPDMVASRLG 96
           FPAILIFGDSTVDTGNNN+ + TIF+A +FPYG + P   + GRFS+GKLI D++A++L 
Sbjct: 33  FPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYGMDLPDGKANGRFSNGKLISDIIATKLN 92

Query: 97  IKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLTAIISRVIPVMKQINLFKNYTERLQK 156
           IKEF+PPFL P LS+ D+ TGV FASAG G+D+LT++ ++ I V +Q N+FK+Y  RL+ 
Sbjct: 93  IKEFIPPFLQPNLSDQDILTGVCFASAGAGYDDLTSLSTQAIRVSEQPNMFKSYIARLKG 152

Query: 157 IVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTRKLQYN-ISGYQDFIQSRLQTLIKKI 216
           IVG  ++  I+ NAFVV+SAG NDF +N+Y+IP+R+L+Y  ISGYQDFI  RL+  ++++
Sbjct: 153 IVGDKKAMEIINNAFVVVSAGPNDFILNYYEIPSRRLEYPFISGYQDFILKRLENFVREL 212

Query: 217 YRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSCIKEQNSDSKAYNQKLSKLLKNLQRK 276
           Y LG RN++V GLPP+GCLPI  T   RN F R C++  N DS  YN+KL  LL  ++  
Sbjct: 213 YSLGVRNVLVGGLPPMGCLPIHMTAKFRNIF-RFCLEHHNKDSVLYNEKLQNLLPQIEAS 272

Query: 277 LHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCCGSGLNEAGPLCNSITPTCKNPSKFM 336
           L GSK  YAD+Y P+ +M+ NP KYGF+ T +GCCG+G  E   +CN  +P C+N S+F+
Sbjct: 273 LPGSKFLYADVYNPMMEMIQNPSKYGFKETKRGCCGTGFLETSFMCNVFSPVCQNRSEFL 332

Query: 337 FWDSIHPTEAAYKIIADSL 354
           F+DSIHP+EA Y +I + L
Sbjct: 333 FFDSIHPSEATYNVIGNLL 350

[Close]

BLAST of CaUC02G040850 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 373.6 bits (958), Expect = 1.8e-103
Identity = 176/344 (51.16%), Postives = 247/344 (71.80%), Query Frame = 0


Query: 12  LSFHIIWLLFLSKPGSALKPKIPRSFPAILIFGDSTVDTGNNNFIS-TIFKANYFPYGKN 71
           ++F +     LS   +A        FPAILIFGDSTVDTGNNN+ S TIFKA + PYG +
Sbjct: 7   ITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGID 66

Query: 72  FPGHVSTGRFSDGKLIPDMVASRLGIKEFVPPFLDPKLSNDDVKTGVSFASAGTGFDNLT 131
            P H ++GRF++GK+  D++A++L IK+FVPPFL P LS+ ++ TGV FASAG G+D+ T
Sbjct: 67  LPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGAGYDDHT 126

Query: 132 AIISRVIPVMKQINLFKNYTERLQKIVGASQSRRILKNAFVVISAGTNDFNVNFYDIPTR 191
           ++ ++ I V+ Q  +FKNY  RL+ IVG  ++  I+KNA VVISAG NDF +N+YDIP+R
Sbjct: 127 SLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNYYDIPSR 186

Query: 192 KLQY-NISGYQDFIQSRLQTLIKKIYRLGCRNMVVAGLPPIGCLPIQQTMSIRNPFNRSC 251
           +L++ +ISGYQDF+  RL   ++++Y LGCR ++V GLPP+GCLPIQ T   RN   R C
Sbjct: 187 RLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRNAL-RFC 246

Query: 252 IKEQNSDSKAYNQKLSKLLKNLQRKLHGSKIFYADIYTPINDMLNNPKKYGFERTDKGCC 311
           ++++N DS  YNQKL  LL  ++  L GSKI Y+++Y P+ DM+ NP KYGF+ T +GCC
Sbjct: 247 LEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKETKRGCC 306

Query: 312 GSGLNEAGPLCNSITPTCKNPSKFMFWDSIHPTEAAYKIIADSL 354
           G+G  E   +CN+ +PTC+N S+F+F+DSIHP+EA Y  + + L
Sbjct: 307 GTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATYNYMGNFL 349

The following BLAST results are available for this feature:

BLAST of CaUC02G040850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151059.1	6.6e-161	77.50	GDSL esterase/lipase At2g30310 [Cucumis sativus] >KGN63338.1 hypothetical protei...	[more]
XP_016902930.1	2.5e-160	77.40	PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0066338.1 GDSL...	[more]
XP_022947820.1	9.9e-157	75.28	GDSL esterase/lipase At2g30310-like [Cucurbita moschata]	[more]
XP_023533087.1	1.7e-156	75.84	GDSL esterase/lipase At2g30310-like [Cucurbita pepo subsp. pepo]	[more]
XP_023006812.1	4.9e-156	75.00	GDSL esterase/lipase At2g30310-like [Cucurbita maxima]	[more]

BLAST of CaUC02G040850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22927	7.0e-105	57.41	GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2...	[more]
O22918	5.9e-104	54.74	GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3...	[more]
Q9SIQ2	5.9e-104	55.49	GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3...	[more]
Q9LMJ3	1.3e-103	52.31	GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2...	[more]
Q9SIQ3	1.9e-102	54.55	GDSL esterase/lipase At2g31540 OS=Arabidopsis thaliana OX=3702 GN=At2g31540 PE=2...	[more]

BLAST of CaUC02G040850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LNE2	3.2e-161	77.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431080 PE=3 SV=1	[more]
A0A5A7VF47	1.2e-160	77.40	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602...	[more]
A0A1S4E4N0	1.2e-160	77.40	GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501132 PE=3...	[more]
A0A6J1G7Z0	4.8e-157	75.28	GDSL esterase/lipase At2g30310-like OS=Cucurbita moschata OX=3662 GN=LOC11145158...	[more]
A0A5A7UVN0	2.4e-156	75.14	GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852...	[more]

BLAST of CaUC02G040850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30310.1	5.0e-106	57.41	GDSL-like Lipase/Acylhydrolase family protein	[more]
AT2G30220.1	4.2e-105	54.74	GDSL-like Lipase/Acylhydrolase family protein	[more]
AT1G06990.1	9.4e-105	52.31	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT2G31540.1	1.4e-103	54.55	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT2G24560.1	1.8e-103	51.16	GDSL-like Lipase/Acylhydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 250..270
None	No IPR available	PANTHER	PTHR45642:SF30	GDSL-LIKE LIPASE/ACYLHYDROLASE	coord: 35..352
None	No IPR available	PANTHER	PTHR45642	GDSL ESTERASE/LIPASE EXL3	coord: 35..352
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 32..357
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 40..352 e-value: 3.2E-36 score: 125.9
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 30..359 e-value: 1.1E-80 score: 273.5
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 38..351 e-value: 3.14876E-144 score: 409.313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC02G040850.1	CaUC02G040850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds