CaUC02G029750 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC02G029750
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	signal recognition particle 43 kDa protein, chloroplastic
Location	Ciama_Chr02: 3881595 .. 3886087 (-)
RNA-Seq Expression	CaUC02G029750
Synteny	CaUC02G029750

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTCGGGCGAAACCCATTCCCAGGGTTGTGCATGTACCCCACTGGGTAAAATAAAATCTTAAAAATATCATAAAGGGAATCCAATGTATATTTTAAGGTTATAATATTACGATTTTGTACTTGTTTCATTTCGGAAAAGACATTTAGTCGTTTAAAGTTAACTTTTATCAAATTTGTTATATAATAATAGTAATAATACTTTCTTTTTCAAAAGACGTGAATATATTTTAAAATCTTACCATCAATACTTTTTTTTCTTCTTTCTTTAAAAAATCAACAATAAACTAATAGTATAATTTTTTTTTTTTTTTTTTTGACATTTATTAAAAGTATATTGACAAAAAGACCAAATTGGTATTTTAAAATATGATGATAGGTATATTGTGCTAATATAATTATGTCTTAAGTAATTTTTAAATTGTATATTATGTTTAAGTCCAAGGTTTTAAATGTCGATATCTATTTTATGGAATTATAATATTAGTAAAAGTATATATAAAATGTCAATAATGTTTGTTGTTGTTTTTTTTTTTTTTTTTTAAATTTGTTGTATTTCATCACGATGATTCATCTTTCATGTCAATTAGGGGTGTTCCTTCCAATCGTAGAAATTGAACCGAAACCAAAACCGAAACAGAACGATAATGCATAGAAACCGAATTCTACGGTTTGGTTCGGTTTGTTTAAATGTAAAACTGTTTTTTTGTGGTTCGATTCGGTTTGGGCCATGAAGTTGAAACCAAAATCGAATCGATTATAATATTATTAAAATATAGGTTAAAAAACACTTTTAATCCCTGAAAAGTAAGGAAAGGAAGCGAGAGCGAGAGTGAGAGTGAGAGCACACATGTCATTTTATCATAAACATCCTCTATCTCTCTCGCTCTCACTCTCGCTTATTTTGAATTATAGGATGAAAAGAGAAGGAAAATAAATAAATAAACCACAAAACCGAACCGCATCGTATATATGTCGTTCGGTTCAAGCTAAACATGCAGTTTAGTTCGATTTCTGTATAAAAGAATTTCAAAATTTGTGGTTCGGTTTGGTTTAAGCCCCAAACCGAACTGAACTGTGAACACCCATAATATCAATATCTAACCCGAACATAAATACGTTGATGAAAATAACACTTTCATCACATTCATCTTAAACAAACTCTAAATTTTTTCTTCTTTCTTCCTAACCAAACTCTTTCCATTTCTTCATTCTATCTTATTTCTTTAGGAGATACCTAAAAAAGTTGTATACATTGCTTAGTAATCAACGAACATTCCCCACTGAAAGCAAAGAAAAATCAGTGTTGGAGTTGGACTAAAAACCCTAAAAAATGAGAAGCGTTTCATTTTAGCAGCCAAATGGTATAATGATGGAAGCCTGTGGGCTGGGCCTAGCCAAAAGCCCACTACCATAATAACAAACAAAGGCCCGTCCACCACCGTCATTGGGCTGAATTGTCAAATATACACACAAATGGGACTATTTTGCAAATTATATTTCACAAATGTCATCTTTTTAGAGGAATTATGGTATATAGTTATGAATAAATGCTTTTCTTTATTAGAGCTTTTGCGAGTTTGAATACCATAATTTTAGGAGAAGAGTGTGAATACTTTTCTTTCTACTTATCCAACTCTTTGAGTGTAGTGTTTATTGAGTCTAATAGCGATAAATGATGTAACTCTTCAAGATCTTCAAGATGGGGCATGATTCTTCGAGTGCATGTGTCTTTTGAGTCAATAGATACAATTGATATTAATGTATTGTGAATATCACAAACTCAAGGGAGGTAGTTACATACTGAACATTTGCATTCTTTTACTTGTGGGGAAGATACATGCGTACAATAATAATAATAAATACGTTTGCTTACAATATTAAAAAAATTACTTAAAAAAAGTTAGTACAACAAATTAATTCTTTTATAAATTGTAAATAAGTTTATAAAAGGGAAATTATCTTAAATGGCAAAACTGTTGAAAATATTTACAACTAATAACAAAATACACAGTCTATTTGCGATAAACCGGGATAGACAATGAAATTTTGCTATATTTATAAATATTTTGGTTGTTTTTGTTATATTTGAAAAGAACCATTTATAAAATCGAGTTTAGAAAATTTTAACACAAAAAAGTTATAAAGAAAAAACAAATATTTTGGAGACAAAAAGGTTATTAAAATTTTCAAATACTGAGGATGGACAAAAATTTGAAAGAGAACATTTTAGTAAGGGAAACAAAGTCAGAAAGAGGAATAAAATTTTAGAGAGATAAAAAATACAGATAATTAAAGAATTTTTTTTCTTTTGACAAAAAGACTAGAAAAAATGAGAAAAATAAAAGAAAAAGAATAGCCTAGAAGGACGAATAAAATAAAATTATTTTTATATTATTTCTCTGATGACATCAAATCTTATTTAAATTCTACATTGATTTTGGATACAATTTGAAATAAGATTGAGTTATAACATAGATAATTTAACTTATAAGTTTGGTTTTGTTTTACTTATTATATTGTAGTTGTGAAAAATAAAATATTTGTATATGTTATAGTATATGTTTTCACTTAATTTTTTGACAGTTGAATATTATTTTAAAACTTTAAATTCAAAATTTTGACATTTTCACATGCAATGCATGTATTCTATGGAAAAATAGGGAAAAAAAAAGATATATATAATTTTTTGAAACTAGGTTAGAACTTTTAATTGGCAACCAAACACCCGAGTAAAGGAATGAGGAAAGGGAGCAAACAACTTTGATTTGTAATTGCTTTTGAGGAGATAGAAGCAATTACAGGCAAACTTTAACCTTTTTTTGTCATTCTCAAAATCACTCTATGACATGGTTTTAATCGTTCAAAACTAATTTTGATGATATGAAAATTACATATAAAAATTAAAGTTAAATATTAAATTAATTTTGAGTGATTAATCACTTTCAAATATTAAATATGTACTATTTAGGATTGAATTTTTATAAATCAAAATTTAAAAATTAAATTGTTAATATAAGGGCGAAAGTTTAGGGATAAAAAGGTAAATTTCAACCATCAACAATCCAATTGAAAGGTAAAAGGTGTGCTGTAAAAAAAGAAAAAAAACTGCACAGTACAAAATATAGAACTCTCTAACTAAATGACAGCCATGGATTCCATCCAATTCCTTATCCATAATTTTCTCTTATCTCAGATTTTGCATACCCCACCTTCCAAAACGACATCGTTTGCCCTCTCCAAACCCTTCTTCCTCCACACTTCCCTTCTCCTTCTTCTTCTTCACAATCACAACCACATCCCCTTCCACCTCCGCCGTGCCTCCCTCCATGGACGCCGTCTTCGTAAACCAATCTCTCTCTCGCCTTAAACTTTCCCCTAAGCCCCCTCTCCCATTCCCCCCTTCTTCCTCTCGAATCCCCCATCTCAATCTCCGCCGCAACCTCAAACATTTCACCCCTTCCTACGCCGTACAAAGCGATCAACCCCTCACACAACAAACCCAGAAATCAGAAGACGATGAATCGTACGGCGAAGTCGATGGAATCATCGGCAGTCGTGCTCTGTCCGACGCTACTGGCATGGAGTATCTAATTAAATGGAAAGATGGGCATTCCCCATCATGGGTTCCTTCCGATTTCATCGCTAAAGATGTTGTCGCCGAGTACGAAACTCCTTGGTGGACCGCCGCCAAGAAAGCCGACGAATCTGCTTTGAAATCGATGATAGATTCTGCGGACGGACGAGATTTCGACGCTGTGGATGAAGATGGGAGGACCGCTCTTCTGTTTGTTGCTGGACTTGGGTCTAATCCGTGTGTGAGAATGTTAGCGGAAGCCGGTGCGAATCTCGATCACCGTGACAACAGCGGTGGGTTTACAGCGTTGCATATGGCAGCTGGGTATGTGAAACCAGAGACGGTGGAGTTGTTGGTGGAATTGGGAGCGGATCCGGAAATTGAGGACGATAAGGGTCGGACGCCATTGGTGTTGGCAAGGGAGATATTGAAAGCGACGCCGAGGGTTCAGTTTATGAGGAGATTGGGGTTGGAGAGAGTGATCGGTGCGGTGGAGAGGGTGGTTTATGAGTATGCGGAGGTGGAACAATTGCTGGAGAAGAGAGGGAAGGGGGAGAATTTGGAGTATTTGGTAAAATGGAAAGACGGAGAGGATAATGAATGGGTGAAAGTGGGGTTGATTGCGGAGGATTTGGTGGCGGATTTCGAAGCAGGGTTGGAGTATGCGGTGGCGGAGGCGGTGGTCGGAAAAAGAGTTGGAGATGATGGGAAGATGGAGTATCTTGTTAAATGGACGGATATACAAGATGCCACGTGGGAGCCTCAGGCTAATGTGGATCCTGATTTGGTTAACGAGTTTGAGAAGGCCCAGGCCCAACCCCAGGCCCAATCCAGTAATGGTAGTGCCCTTTGAAAAAGCCTTTTCCCCTCCAAGTATGTATTTAAACTAGGGATTTTTTTTAAGATATTGTTATTG

mRNA sequence

ATGCTTCGGGCGAAACCCATTCCCAGGGTTGTGCATGTACCCCACTGGCCATGGATTCCATCCAATTCCTTATCCATAATTTTCTCTTATCTCAGATTTTGCATACCCCACCTTCCAAAACGACATCGTTTGCCCTCTCCAAACCCTTCTTCCTCCACACTTCCCTTCTCCTTCTTCTTCTTCACAATCACAACCACATCCCCTTCCACCTCCGCCGTGCCTCCCTCCATGGACGCCGTCTTCGTAAACCAATCTCTCTCTCGCCTTAAACTTTCCCCTAAGCCCCCTCTCCCATTCCCCCCTTCTTCCTCTCGAATCCCCCATCTCAATCTCCGCCGCAACCTCAAACATTTCACCCCTTCCTACGCCGTACAAAGCGATCAACCCCTCACACAACAAACCCAGAAATCAGAAGACGATGAATCGTACGGCGAAGTCGATGGAATCATCGGCAGTCGTGCTCTGTCCGACGCTACTGGCATGGAGTATCTAATTAAATGGAAAGATGGGCATTCCCCATCATGGGTTCCTTCCGATTTCATCGCTAAAGATGTTGTCGCCGAGTACGAAACTCCTTGGTGGACCGCCGCCAAGAAAGCCGACGAATCTGCTTTGAAATCGATGATAGATTCTGCGGACGGACGAGATTTCGACGCTGTGGATGAAGATGGGAGGACCGCTCTTCTGTTTGTTGCTGGACTTGGGTCTAATCCGTGTGTGAGAATGTTAGCGGAAGCCGGTGCGAATCTCGATCACCGTGACAACAGCGGTGGGTTTACAGCGTTGCATATGGCAGCTGGGTATGTGAAACCAGAGACGGTGGAGTTGTTGGTGGAATTGGGAGCGGATCCGGAAATTGAGGACGATAAGGGTCGGACGCCATTGGTGTTGGCAAGGGAGATATTGAAAGCGACGCCGAGGGTTCAGTTTATGAGGAGATTGGGGTTGGAGAGAGTGATCGGTGCGGTGGAGAGGGTGGTTTATGAGTATGCGGAGGTGGAACAATTGCTGGAGAAGAGAGGGAAGGGGGAGAATTTGGAGTATTTGGTAAAATGGAAAGACGGAGAGGATAATGAATGGGTGAAAGTGGGGTTGATTGCGGAGGATTTGGTGGCGGATTTCGAAGCAGGGTTGGAGTATGCGGTGGCGGAGGCGGTGGTCGGAAAAAGAGTTGGAGATGATGGGAAGATGGAGTATCTTGTTAAATGGACGGATATACAAGATGCCACGTGGGAGCCTCAGGCTAATGTGGATCCTGATTTGGTTAACGAGTTTGAGAAGGCCCAGGCCCAACCCCAGGCCCAATCCAGTAATGGTAGTGCCCTTTGAAAAAGCCTTTTCCCCTCCAAGTATGTATTTAAACTAGGGATTTTTTTTAAGATATTGTTATTG

Coding sequence (CDS)

ATGCTTCGGGCGAAACCCATTCCCAGGGTTGTGCATGTACCCCACTGGCCATGGATTCCATCCAATTCCTTATCCATAATTTTCTCTTATCTCAGATTTTGCATACCCCACCTTCCAAAACGACATCGTTTGCCCTCTCCAAACCCTTCTTCCTCCACACTTCCCTTCTCCTTCTTCTTCTTCACAATCACAACCACATCCCCTTCCACCTCCGCCGTGCCTCCCTCCATGGACGCCGTCTTCGTAAACCAATCTCTCTCTCGCCTTAAACTTTCCCCTAAGCCCCCTCTCCCATTCCCCCCTTCTTCCTCTCGAATCCCCCATCTCAATCTCCGCCGCAACCTCAAACATTTCACCCCTTCCTACGCCGTACAAAGCGATCAACCCCTCACACAACAAACCCAGAAATCAGAAGACGATGAATCGTACGGCGAAGTCGATGGAATCATCGGCAGTCGTGCTCTGTCCGACGCTACTGGCATGGAGTATCTAATTAAATGGAAAGATGGGCATTCCCCATCATGGGTTCCTTCCGATTTCATCGCTAAAGATGTTGTCGCCGAGTACGAAACTCCTTGGTGGACCGCCGCCAAGAAAGCCGACGAATCTGCTTTGAAATCGATGATAGATTCTGCGGACGGACGAGATTTCGACGCTGTGGATGAAGATGGGAGGACCGCTCTTCTGTTTGTTGCTGGACTTGGGTCTAATCCGTGTGTGAGAATGTTAGCGGAAGCCGGTGCGAATCTCGATCACCGTGACAACAGCGGTGGGTTTACAGCGTTGCATATGGCAGCTGGGTATGTGAAACCAGAGACGGTGGAGTTGTTGGTGGAATTGGGAGCGGATCCGGAAATTGAGGACGATAAGGGTCGGACGCCATTGGTGTTGGCAAGGGAGATATTGAAAGCGACGCCGAGGGTTCAGTTTATGAGGAGATTGGGGTTGGAGAGAGTGATCGGTGCGGTGGAGAGGGTGGTTTATGAGTATGCGGAGGTGGAACAATTGCTGGAGAAGAGAGGGAAGGGGGAGAATTTGGAGTATTTGGTAAAATGGAAAGACGGAGAGGATAATGAATGGGTGAAAGTGGGGTTGATTGCGGAGGATTTGGTGGCGGATTTCGAAGCAGGGTTGGAGTATGCGGTGGCGGAGGCGGTGGTCGGAAAAAGAGTTGGAGATGATGGGAAGATGGAGTATCTTGTTAAATGGACGGATATACAAGATGCCACGTGGGAGCCTCAGGCTAATGTGGATCCTGATTTGGTTAACGAGTTTGAGAAGGCCCAGGCCCAACCCCAGGCCCAATCCAGTAATGGTAGTGCCCTTTGA

Protein sequence

MLRAKPIPRVVHVPHWPWIPSNSLSIIFSYLRFCIPHLPKRHRLPSPNPSSSTLPFSFFFFTITTTSPSTSAVPPSMDAVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQKSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNSGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLGLERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFEAGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQAQPQAQSSNGSAL

Homology

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BLAST of CaUC02G029750 vs. NCBI nr
Match: XP_038888741.1 (signal recognition particle 43 kDa protein, chloroplastic [Benincasa hispida])

HSP 1 Score: 685.3 bits (1767), Expect = 3.6e-193
Identity = 349/375 (93.07%), Postives = 358/375 (95.47%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFPP-SSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQ 136
           MDAVFVNQSLSRLKLSPKPPLPFPP SSSR PHLNLRRN KHFTP YAVQSDQPLTQQTQ
Sbjct: 1   MDAVFVNQSLSRLKLSPKPPLPFPPSSSSRNPHLNLRRNFKHFTP-YAVQSDQPLTQQTQ 60

Query: 137 KSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT 196
           KSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT
Sbjct: 61  KSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT 120

Query: 197 AAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDN 256
           AAKKADESALKS+ID+ DGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAG NLDHRDN
Sbjct: 121 AAKKADESALKSLIDAGDGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGVNLDHRDN 180

Query: 257 SGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLG 316
           SGGFTALH+AAGYVKPETVELLVELGADPEIED+KGRTPLVLA+EILKATPRVQFMRR+G
Sbjct: 181 SGGFTALHLAAGYVKPETVELLVELGADPEIEDEKGRTPLVLAKEILKATPRVQFMRRIG 240

Query: 317 LERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFE 376
           LERVIGAVE+VVYEYAEVE+LLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFE
Sbjct: 241 LERVIGAVEKVVYEYAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFE 300

Query: 377 AGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQAQP--- 436
           AGLEYAVAEAVVGKR GDDGKMEYLVKWTDIQDATWEPQ NVDPDL+ EFEKAQAQP   
Sbjct: 301 AGLEYAVAEAVVGKRFGDDGKMEYLVKWTDIQDATWEPQDNVDPDLIAEFEKAQAQPQAQ 360

Query: 437 -----QAQSSNGSAL 443
                QAQSSNGSAL
Sbjct: 361 PQAQSQAQSSNGSAL 374

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BLAST of CaUC02G029750 vs. NCBI nr
Match: XP_008447899.1 (PREDICTED: signal recognition particle 43 kDa protein, chloroplastic [Cucumis melo])

HSP 1 Score: 631.3 bits (1627), Expect = 6.1e-177
Identity = 318/358 (88.83%), Postives = 336/358 (93.85%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFP-PSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQ 136
           M+AVF+NQSLSRLKL PKPPLP P P  S IPHLNLRRN  HFT +YAVQSDQP+TQQT 
Sbjct: 1   MEAVFLNQSLSRLKLPPKPPLPLPIPFPSTIPHLNLRRNFTHFT-AYAVQSDQPITQQTY 60

Query: 137 KSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT 196
           K EDDESYGEVDGIIGSRAL DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT
Sbjct: 61  KPEDDESYGEVDGIIGSRALPDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT 120

Query: 197 AAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDN 256
           AAKKAD+SAL+S+IDS D RDFDAVDEDGRTALLFV+GLGS  CVR+LAEAG NL+HRDN
Sbjct: 121 AAKKADKSALESLIDSGDERDFDAVDEDGRTALLFVSGLGSVECVRILAEAGVNLNHRDN 180

Query: 257 SGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLG 316
           SGGFTALHMAAGYVKPETVELLVELGADPEIED+KGRT L LA+EILKATPRVQFMRRLG
Sbjct: 181 SGGFTALHMAAGYVKPETVELLVELGADPEIEDEKGRTALELAKEILKATPRVQFMRRLG 240

Query: 317 LERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFE 376
           LE+VIGAVE+VVYEYAEVE+LLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVAD+E
Sbjct: 241 LEKVIGAVEKVVYEYAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADYE 300

Query: 377 AGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQAQPQ 434
           AGLEYAVAEAVVGKRVGD+GKMEYLVKWTDIQDATWEP  NVDPDL+NEFEKAQAQ Q
Sbjct: 301 AGLEYAVAEAVVGKRVGDEGKMEYLVKWTDIQDATWEPLDNVDPDLINEFEKAQAQAQ 357

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BLAST of CaUC02G029750 vs. NCBI nr
Match: XP_004153574.1 (signal recognition particle 43 kDa protein, chloroplastic [Cucumis sativus])

HSP 1 Score: 627.1 bits (1616), Expect = 1.2e-175
Identity = 316/355 (89.01%), Postives = 333/355 (93.80%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQK 136
           M+AVF+NQSLSRLKL PK PLPFP   S IPHLNLRRN  HFT +YAVQSDQP+TQQTQK
Sbjct: 1   MEAVFLNQSLSRLKLPPKTPLPFP---SPIPHLNLRRNFTHFT-AYAVQSDQPITQQTQK 60

Query: 137 SEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTA 196
            EDDESYGEVDGIIGSRAL DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTA
Sbjct: 61  PEDDESYGEVDGIIGSRALPDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTA 120

Query: 197 AKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNS 256
           AKKADESALKS+IDS D RDFDAVDEDGRTALLFV+GLGS  CVR+LA+AG NL+HRDNS
Sbjct: 121 AKKADESALKSLIDSGDERDFDAVDEDGRTALLFVSGLGSVECVRILADAGVNLNHRDNS 180

Query: 257 GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLGL 316
           GGFTALHMAAGYVKPETVELLVELGADPEI+D+KGRT L LA+EILKATPRVQFMRRLGL
Sbjct: 181 GGFTALHMAAGYVKPETVELLVELGADPEIDDEKGRTALELAKEILKATPRVQFMRRLGL 240

Query: 317 ERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFEA 376
           E+VI AVE+VVYEYAEVE+LLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLV D+EA
Sbjct: 241 EKVIRAVEKVVYEYAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVGDYEA 300

Query: 377 GLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQAQ 432
           GLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEP  NVDPDL+NEFEKAQAQ
Sbjct: 301 GLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPLDNVDPDLINEFEKAQAQ 351

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BLAST of CaUC02G029750 vs. NCBI nr
Match: XP_022928488.1 (signal recognition particle 43 kDa protein, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 604.7 bits (1558), Expect = 6.2e-169
Identity = 323/423 (76.36%), Postives = 352/423 (83.22%), Query Frame = 0


Query: 35  IPHLPKRHR------LPSPNPSSSTLPFSFFFFTITTTSPSTSAVPPSMDAVFVNQSLSR 94
           +P   KRHR      LP P+   S +              S +A  P+MDAVFVNQSLSR
Sbjct: 22  VPTSTKRHRSLSATLLPLPSQPHSAI-------------SSATAFSPAMDAVFVNQSLSR 81

Query: 95  LKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQK----SEDDESYG 154
           LKL PKPPLP  PS   I HL+LRR  K FT +YAV  ++PLTQQTQ+     EDDESYG
Sbjct: 82  LKLPPKPPLPKLPSP--ILHLHLRRTFKSFT-AYAV--NEPLTQQTQQPDNFPEDDESYG 141

Query: 155 EVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESA 214
           EVDGIIGSRAL+DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESA
Sbjct: 142 EVDGIIGSRALADATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESA 201

Query: 215 LKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNSGGFTALHM 274
           LKS+ID+AD RDF+AVDEDGRTALLFVAGLGS PC+R+LAEAGA+LDHRD SGGFTALHM
Sbjct: 202 LKSLIDAADSRDFNAVDEDGRTALLFVAGLGSEPCIRILAEAGADLDHRDKSGGFTALHM 261

Query: 275 AAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLGLERVIGAVE 334
           AAGYVKP TVELL+ELGADPE++DDKGRTPLVLAREI+KATPRVQF RRLGLERVIGA+E
Sbjct: 262 AAGYVKPGTVELLLELGADPEVQDDKGRTPLVLAREIVKATPRVQFARRLGLERVIGALE 321

Query: 335 RVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFEAGLEYAVAE 394
           RVV+E+AEVE+LLEKRGKGENLEYLVKWKDGEDNEWVK  LIAEDLVADFEAGLEYAVAE
Sbjct: 322 RVVFEFAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKAALIAEDLVADFEAGLEYAVAE 381

Query: 395 AVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQ--------AQPQAQS 440
           AVVGKRVGDDGKMEYLVKWTDI+DATWEP+ NVD DLV EFE  Q        +  +AQS
Sbjct: 382 AVVGKRVGDDGKMEYLVKWTDIEDATWEPEGNVDSDLVTEFETGQKSVGPGPISDLEAQS 426

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BLAST of CaUC02G029750 vs. NCBI nr
Match: XP_023529738.1 (signal recognition particle 43 kDa protein, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.4 bits (1544), Expect = 2.6e-167
Identity = 311/375 (82.93%), Postives = 333/375 (88.80%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQK 136
           MDAVFVNQSLSRLKL PKPPLP  PS     HL+LRR  K FT +YAV  ++PLTQQTQ+
Sbjct: 1   MDAVFVNQSLSRLKLPPKPPLPKLPSPILHLHLHLRRTFKSFT-AYAV--NEPLTQQTQQ 60

Query: 137 ----SEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETP 196
                EDDESYGEVDGIIGSRAL+DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETP
Sbjct: 61  PDDFPEDDESYGEVDGIIGSRALADATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETP 120

Query: 197 WWTAAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDH 256
           WWTAAKKADESALKS+ID+AD RDF+AVDED RTALLFVAGLGS PCVR+LAEAGANLDH
Sbjct: 121 WWTAAKKADESALKSLIDAADSRDFNAVDEDSRTALLFVAGLGSEPCVRILAEAGANLDH 180

Query: 257 RDNSGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMR 316
           RD SGGFTALHMAAGYVKP TVELL+ELGADPE++DDKGRTPLVLAREI+KATPRVQF R
Sbjct: 181 RDKSGGFTALHMAAGYVKPGTVELLLELGADPEVQDDKGRTPLVLAREIVKATPRVQFAR 240

Query: 317 RLGLERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVA 376
           RLGLERVIGA+ERVV+E+AEVE+LLEKRGKGENLEYLVKWKDGEDNEWVK  LIAEDLVA
Sbjct: 241 RLGLERVIGALERVVFEFAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKAALIAEDLVA 300

Query: 377 DFEAGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQ--- 436
           DFEAGLEYAVAEAVVGKRVGDDGKMEYLVKWTDI+DATWEP+ NVD DLV EFE  Q   
Sbjct: 301 DFEAGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIEDATWEPEGNVDSDLVTEFETGQKSV 360

Query: 437 -----AQPQAQSSNG 440
                +  +AQSSNG
Sbjct: 361 GPGPISDLEAQSSNG 372

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BLAST of CaUC02G029750 vs. ExPASy Swiss-Prot
Match: O22265 (Signal recognition particle 43 kDa protein, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAO PE=1 SV=2)

HSP 1 Score: 407.5 bits (1046), Expect = 1.9e-112
Identity = 220/362 (60.77%), Postives = 275/362 (75.97%), Query Frame = 0


Query: 79  AVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSY--AVQSD-QPLTQQTQ 138
           A+ ++QSLSR+KLSP        S+     L +RR    F  +   AVQ + +  T   +
Sbjct: 12  ALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVE 71

Query: 139 KSEDDE----SYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYET 198
           ++E+D+    SYGEV+ IIGSR   +   MEYLI+WKDGHSPSWVPS +IA DVV+EYET
Sbjct: 72  EAEEDDESSSSYGEVNKIIGSRTAGEG-AMEYLIEWKDGHSPSWVPSSYIAADVVSEYET 131

Query: 199 PWWTAAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLD 258
           PWWTAA+KADE AL  +++    RD DAVDE+GRTALLFVAGLGS+ CVR+LAEAGA+LD
Sbjct: 132 PWWTAARKADEQALSQLLED---RDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLD 191

Query: 259 HRDNSGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPR---V 318
           HRD  GG TALHMAAGYV+PE VE LVELGAD E+ED++G T L LAREILK TP+   +
Sbjct: 192 HRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPM 251

Query: 319 QFMRRLGLERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAE 378
           QF RR+GLE+VI  +E  V+EYAEV++++EKRGKG+++EYLV+WKDG D EWVK   +AE
Sbjct: 252 QFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAE 311

Query: 379 DLVADFEAGLEYAVAEAVVGKRVGDDGK-MEYLVKWTDIQDATWEPQANVDPDLVNEFEK 430
           D+  D+E GLEYAVAE+V+GKRVGDDGK +EYLVKWTD+ DATWEPQ NVD  LV  +++
Sbjct: 312 DVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQ 369

[Close]

BLAST of CaUC02G029750 vs. ExPASy Swiss-Prot
Match: Q8LSQ2 (Probable signal recognition particle 43 kDa protein, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0131900 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.1e-91
Identity = 188/374 (50.27%), Postives = 245/374 (65.51%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQ--PLTQQT 136
           M+AV  + SLSRLK  P P     P+ S      LR   +  T + AV  DQ  P    +
Sbjct: 1   MEAVLRHPSLSRLK-PPNPNAQRTPALSITVPFRLRLPNRRLTAA-AVFQDQTNPRNPAS 60

Query: 137 QKSEDDESYGEVDGIIGSRALSD-----------ATGMEYLIKWKDGHSPSWVPSDFIAK 196
           +  +DDE+YGEVD I+ SR + +            T  EYL++WKDGH PSW+P++ IA 
Sbjct: 61  KGGDDDEAYGEVDRIVSSRTIKNPVFAEDGSATTVTATEYLVEWKDGHEPSWIPAEAIAA 120

Query: 197 DVVAEYETPWWTAAKKADESALKSMI-DSADGRDFDAVDEDGRTALLFVAGLGSNPCVRM 256
           DVVAEYETPWWTAAKKAD + + +++ D    RD DA D  GRTA+ F AGLGS  CVR 
Sbjct: 121 DVVAEYETPWWTAAKKADAAEITALLADETLRRDPDAEDAQGRTAMHFAAGLGSEECVRA 180

Query: 257 LAEAGANLDHRDNS-GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREI 316
           LAEAGA++   + + GG T LH+A GY +P  V  L+ELGA+PE  D +GRTPL L +++
Sbjct: 181 LAEAGADVGRPERAGGGLTPLHIAVGYGRPAAVRALLELGAEPEAPDGQGRTPLELVQDV 240

Query: 317 LKATPR---VQFMRRLGLERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDN 376
           L  TP+     F RRL LE     +E+ VYE+ EVE++++ RG+G+  EYLV+W+DG D 
Sbjct: 241 LAKTPKGNPATFERRLALEAAAKELEKAVYEWGEVEKVVDGRGEGKWREYLVEWRDGGDR 300

Query: 377 EWVKVGLIAEDLVADFEAGLEYAVAEAVVGKR--VGDDGKMEYLVKWTDIQDATWEPQAN 431
           EWV+   +AEDLV DF+AGLEYAVAEAVV KR     +GK EYLVKW DI++ATWEP  N
Sbjct: 301 EWVRAAWVAEDLVKDFDAGLEYAVAEAVVNKREAAEGEGKWEYLVKWVDIEEATWEPAEN 360

[Close]

BLAST of CaUC02G029750 vs. ExPASy Swiss-Prot
Match: Q06527 (Ankyrin homolog OS=Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) OX=572477 GN=Alvin_1094 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 8.8e-09
Identity = 49/127 (38.58%), Postives = 62/127 (48.82%), Query Frame = 0


Query: 188 EYETPWWTAAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAG 247
           E  T    AA K +   L  +I+   G D +A D+ G TALL  A  G    V  LA  G
Sbjct: 163 EGRTALMQAASKGETGVLPLLIEG--GADLEARDKQGATALLIAADQGQAGAVETLAGLG 222

Query: 248 ANLDHRDNSGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPR 307
           A LD  D  G  TAL +A G+     VE L+ +GADP  +D KG T L+ A     AT  
Sbjct: 223 AQLDAVDALGS-TALILAVGHGHVAMVERLLAMGADPNRQDRKGTTALMEA----VATDH 282

Query: 308 VQFMRRL 315
            + + RL
Sbjct: 283 AELIDRL 282

[Close]

BLAST of CaUC02G029750 vs. ExPASy Swiss-Prot
Match: Q9VL06 (E3 ubiquitin-protein ligase Ufd4 OS=Drosophila melanogaster OX=7227 GN=Ufd4 PE=2 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.9e-08
Identity = 36/105 (34.29%), Postives = 61/105 (58.10%), Query Frame = 0


Query: 198 KKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNSG 257
           +  D  AL+  I+S  G D + +D+ G+T L + +  G+   V  L E GA+++    S 
Sbjct: 398 RSKDSEALREAIESG-GIDVNCMDDVGQTLLNWASAFGTLEMVEYLCEKGADVNKGQRS- 457

Query: 258 GFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREIL 303
             ++LH AA + +P   ++L++ GA P++ D+ G+TPL  ARE L
Sbjct: 458 --SSLHYAACFGRPAIAKILLKFGAYPDLRDEDGKTPLDKARERL 498

[Close]

BLAST of CaUC02G029750 vs. ExPASy Swiss-Prot
Match: Q69ZR2 (E3 ubiquitin-protein ligase HECTD1 OS=Mus musculus OX=10090 GN=Hectd1 PE=1 SV=2)

HSP 1 Score: 61.2 bits (147), Expect = 3.3e-08
Identity = 33/103 (32.04%), Postives = 58/103 (56.31%), Query Frame = 0


Query: 198 KKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNSG 257
           +  D  AL   ID+    + + +D+ G+T L + +  G+   V  L E GA+++    S 
Sbjct: 371 RSKDTDALIDAIDTGAAFEVNFMDDVGQTLLNWASAFGTQEMVEFLCERGADVNRGQRS- 430

Query: 258 GFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLARE 301
             ++LH AA + +P+  + L+  GA+P++ D+ G+TPL  ARE
Sbjct: 431 --SSLHYAACFGRPQVAKTLLRHGANPDLRDEDGKTPLDKARE 470

[Close]

BLAST of CaUC02G029750 vs. ExPASy TrEMBL
Match: A0A1S3BHX6 (signal recognition particle 43 kDa protein, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490246 PE=4 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 3.0e-177
Identity = 318/358 (88.83%), Postives = 336/358 (93.85%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFP-PSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQ 136
           M+AVF+NQSLSRLKL PKPPLP P P  S IPHLNLRRN  HFT +YAVQSDQP+TQQT 
Sbjct: 1   MEAVFLNQSLSRLKLPPKPPLPLPIPFPSTIPHLNLRRNFTHFT-AYAVQSDQPITQQTY 60

Query: 137 KSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT 196
           K EDDESYGEVDGIIGSRAL DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT
Sbjct: 61  KPEDDESYGEVDGIIGSRALPDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWT 120

Query: 197 AAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDN 256
           AAKKAD+SAL+S+IDS D RDFDAVDEDGRTALLFV+GLGS  CVR+LAEAG NL+HRDN
Sbjct: 121 AAKKADKSALESLIDSGDERDFDAVDEDGRTALLFVSGLGSVECVRILAEAGVNLNHRDN 180

Query: 257 SGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLG 316
           SGGFTALHMAAGYVKPETVELLVELGADPEIED+KGRT L LA+EILKATPRVQFMRRLG
Sbjct: 181 SGGFTALHMAAGYVKPETVELLVELGADPEIEDEKGRTALELAKEILKATPRVQFMRRLG 240

Query: 317 LERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFE 376
           LE+VIGAVE+VVYEYAEVE+LLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVAD+E
Sbjct: 241 LEKVIGAVEKVVYEYAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADYE 300

Query: 377 AGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQAQPQ 434
           AGLEYAVAEAVVGKRVGD+GKMEYLVKWTDIQDATWEP  NVDPDL+NEFEKAQAQ Q
Sbjct: 301 AGLEYAVAEAVVGKRVGDEGKMEYLVKWTDIQDATWEPLDNVDPDLINEFEKAQAQAQ 357

[Close]

BLAST of CaUC02G029750 vs. ExPASy TrEMBL
Match: A0A0A0K634 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018780 PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 5.6e-176
Identity = 316/355 (89.01%), Postives = 333/355 (93.80%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQK 136
           M+AVF+NQSLSRLKL PK PLPFP   S IPHLNLRRN  HFT +YAVQSDQP+TQQTQK
Sbjct: 1   MEAVFLNQSLSRLKLPPKTPLPFP---SPIPHLNLRRNFTHFT-AYAVQSDQPITQQTQK 60

Query: 137 SEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTA 196
            EDDESYGEVDGIIGSRAL DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTA
Sbjct: 61  PEDDESYGEVDGIIGSRALPDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTA 120

Query: 197 AKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNS 256
           AKKADESALKS+IDS D RDFDAVDEDGRTALLFV+GLGS  CVR+LA+AG NL+HRDNS
Sbjct: 121 AKKADESALKSLIDSGDERDFDAVDEDGRTALLFVSGLGSVECVRILADAGVNLNHRDNS 180

Query: 257 GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLGL 316
           GGFTALHMAAGYVKPETVELLVELGADPEI+D+KGRT L LA+EILKATPRVQFMRRLGL
Sbjct: 181 GGFTALHMAAGYVKPETVELLVELGADPEIDDEKGRTALELAKEILKATPRVQFMRRLGL 240

Query: 317 ERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFEA 376
           E+VI AVE+VVYEYAEVE+LLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLV D+EA
Sbjct: 241 EKVIRAVEKVVYEYAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVGDYEA 300

Query: 377 GLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQAQ 432
           GLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEP  NVDPDL+NEFEKAQAQ
Sbjct: 301 GLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPLDNVDPDLINEFEKAQAQ 351

[Close]

BLAST of CaUC02G029750 vs. ExPASy TrEMBL
Match: A0A6J1EP65 (signal recognition particle 43 kDa protein, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111435281 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 3.0e-169
Identity = 323/423 (76.36%), Postives = 352/423 (83.22%), Query Frame = 0


Query: 35  IPHLPKRHR------LPSPNPSSSTLPFSFFFFTITTTSPSTSAVPPSMDAVFVNQSLSR 94
           +P   KRHR      LP P+   S +              S +A  P+MDAVFVNQSLSR
Sbjct: 22  VPTSTKRHRSLSATLLPLPSQPHSAI-------------SSATAFSPAMDAVFVNQSLSR 81

Query: 95  LKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQK----SEDDESYG 154
           LKL PKPPLP  PS   I HL+LRR  K FT +YAV  ++PLTQQTQ+     EDDESYG
Sbjct: 82  LKLPPKPPLPKLPSP--ILHLHLRRTFKSFT-AYAV--NEPLTQQTQQPDNFPEDDESYG 141

Query: 155 EVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESA 214
           EVDGIIGSRAL+DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESA
Sbjct: 142 EVDGIIGSRALADATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESA 201

Query: 215 LKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNSGGFTALHM 274
           LKS+ID+AD RDF+AVDEDGRTALLFVAGLGS PC+R+LAEAGA+LDHRD SGGFTALHM
Sbjct: 202 LKSLIDAADSRDFNAVDEDGRTALLFVAGLGSEPCIRILAEAGADLDHRDKSGGFTALHM 261

Query: 275 AAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLGLERVIGAVE 334
           AAGYVKP TVELL+ELGADPE++DDKGRTPLVLAREI+KATPRVQF RRLGLERVIGA+E
Sbjct: 262 AAGYVKPGTVELLLELGADPEVQDDKGRTPLVLAREIVKATPRVQFARRLGLERVIGALE 321

Query: 335 RVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFEAGLEYAVAE 394
           RVV+E+AEVE+LLEKRGKGENLEYLVKWKDGEDNEWVK  LIAEDLVADFEAGLEYAVAE
Sbjct: 322 RVVFEFAEVEELLEKRGKGENLEYLVKWKDGEDNEWVKAALIAEDLVADFEAGLEYAVAE 381

Query: 395 AVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQ--------AQPQAQS 440
           AVVGKRVGDDGKMEYLVKWTDI+DATWEP+ NVD DLV EFE  Q        +  +AQS
Sbjct: 382 AVVGKRVGDDGKMEYLVKWTDIEDATWEPEGNVDSDLVTEFETGQKSVGPGPISDLEAQS 426

[Close]

BLAST of CaUC02G029750 vs. ExPASy TrEMBL
Match: A0A6J1JM92 (signal recognition particle 43 kDa protein, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111486468 PE=4 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 2.4e-166
Identity = 316/412 (76.70%), Postives = 349/412 (84.71%), Query Frame = 0


Query: 44  LPSPNPSSSTLPFSFFFFTIT----TTSPSTSAVPPSMDAVFVNQSLSRLKLSPKPPLPF 103
           + SP P + T+P       +     +   S +A  P+MDAVFVNQSLS LKL PKPPLP 
Sbjct: 13  IQSPPPQNDTVPSLQPSLPLPSQPHSAISSATAFSPAMDAVFVNQSLSSLKLPPKPPLPK 72

Query: 104 PPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQK----SEDDESYGEVDGIIGSRAL 163
            PS   I HL+LRR  K FT +YAV  ++PLTQQTQ+     EDDESYGEVDGIIGSR+L
Sbjct: 73  LPSP--ILHLHLRRTFKSFT-AYAV--NEPLTQQTQQPDNFPEDDESYGEVDGIIGSRSL 132

Query: 164 SDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESALKSMIDSADGR 223
           +DATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESALKS+ID+AD R
Sbjct: 133 ADATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYETPWWTAAKKADESALKSLIDAADSR 192

Query: 224 DFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNSGGFTALHMAAGYVKPETVE 283
           DF+AVDEDGRTALLFVAGLGS PCVR+LAEAGA+LDHRD SGGFTALHMAAGYVKP TVE
Sbjct: 193 DFNAVDEDGRTALLFVAGLGSEPCVRILAEAGADLDHRDKSGGFTALHMAAGYVKPGTVE 252

Query: 284 LLVELGADPEIEDDKGRTPLVLAREILKATPRVQFMRRLGLERVIGAVERVVYEYAEVEQ 343
           LL+ELGADPE++DDKGRTPLVLAREI+KATPRVQF RRLGLERVIGA+ERVV+E+AEVE+
Sbjct: 253 LLLELGADPEVQDDKGRTPLVLAREIVKATPRVQFARRLGLERVIGALERVVFEFAEVEE 312

Query: 344 LLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAEDLVADFEAGLEYAVAEAVVGKRVGDDG 403
           LLEKRGKGENLEYLVKWKDGEDNEWVK  LIAEDLVADFEAGLEYAVAEAVVGKR+GDDG
Sbjct: 313 LLEKRGKGENLEYLVKWKDGEDNEWVKAALIAEDLVADFEAGLEYAVAEAVVGKRLGDDG 372

Query: 404 KMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQ--------AQPQAQSSNG 440
           KMEYLVKWTDI+DATWEP+ NVD DL+ EFE  Q        +  +AQSSNG
Sbjct: 373 KMEYLVKWTDIEDATWEPEGNVDSDLIAEFETGQKSVGPGPSSDLEAQSSNG 419

[Close]

BLAST of CaUC02G029750 vs. ExPASy TrEMBL
Match: A0A6J1C248 (signal recognition particle 43 kDa protein, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007575 PE=4 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 2.3e-153
Identity = 286/370 (77.30%), Postives = 317/370 (85.68%), Query Frame = 0


Query: 77  MDAVFVNQSLSRLKLSPK-PPLPFPPSSSRIPHLNLRRNLKHFTPSYAVQSDQPLTQQTQ 136
           MDA+F+NQS+SRLK+SPK  PLP P   SRIP+ NL+ +      + AVQ+DQ L QQ+ 
Sbjct: 1   MDALFLNQSISRLKISPKSSPLP-PLYPSRIPNRNLKFS------ACAVQNDQRLAQQSN 60

Query: 137 ------KSEDDESYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEY 196
                 K  +DE+YGEVDGIIGSRAL D +GMEYLI WKDGH+PSWVPSDFIA+DVVAEY
Sbjct: 61  AAISAGKFNEDETYGEVDGIIGSRALQDGSGMEYLIAWKDGHAPSWVPSDFIARDVVAEY 120

Query: 197 ETPWWTAAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGAN 256
           ETPWWTAAKKADESALKS+ID+ADGRD DAVDEDGRTALLFVAGLGS  CVRMLAE GAN
Sbjct: 121 ETPWWTAAKKADESALKSLIDAADGRDLDAVDEDGRTALLFVAGLGSEACVRMLAEGGAN 180

Query: 257 LDHRDNSGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPRVQ 316
           +DHRDNSGGFTALHMAAGYVKP  VE L++LGA PE+ED+KGRT L LAREILKATPR+Q
Sbjct: 181 VDHRDNSGGFTALHMAAGYVKPGAVEQLLDLGAYPEVEDEKGRTALELAREILKATPRIQ 240

Query: 317 FMRRLGLERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAED 376
           F RR+GLERV+GA+E+ V+EYAEVE LLEKRGKGENLEYLVKWKDGEDNEWVK GLIA D
Sbjct: 241 FGRRMGLERVVGALEKAVFEYAEVEALLEKRGKGENLEYLVKWKDGEDNEWVKAGLIAAD 300

Query: 377 LVADFEAGLEYAVAEAVVGKRVGDDGKMEYLVKWTDIQDATWEPQANVDPDLVNEFEKAQ 436
           LVADFEAGLEYAVAEAV GKR+GDDGKMEYLVKWTDI+DATWEP  NVDPDL+ EFE A 
Sbjct: 301 LVADFEAGLEYAVAEAVAGKRMGDDGKMEYLVKWTDIEDATWEPLDNVDPDLIKEFEDA- 359

Query: 437 AQPQAQSSNG 440
              QAQSSNG
Sbjct: 361 ---QAQSSNG 359

[Close]

BLAST of CaUC02G029750 vs. TAIR 10
Match: AT2G47450.1 (chloroplast signal recognition particle component (CAO) )

HSP 1 Score: 407.5 bits (1046), Expect = 1.3e-113
Identity = 220/362 (60.77%), Postives = 275/362 (75.97%), Query Frame = 0


Query: 79  AVFVNQSLSRLKLSPKPPLPFPPSSSRIPHLNLRRNLKHFTPSY--AVQSD-QPLTQQTQ 138
           A+ ++QSLSR+KLSP        S+     L +RR    F  +   AVQ + +  T   +
Sbjct: 12  ALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVE 71

Query: 139 KSEDDE----SYGEVDGIIGSRALSDATGMEYLIKWKDGHSPSWVPSDFIAKDVVAEYET 198
           ++E+D+    SYGEV+ IIGSR   +   MEYLI+WKDGHSPSWVPS +IA DVV+EYET
Sbjct: 72  EAEEDDESSSSYGEVNKIIGSRTAGEG-AMEYLIEWKDGHSPSWVPSSYIAADVVSEYET 131

Query: 199 PWWTAAKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLD 258
           PWWTAA+KADE AL  +++    RD DAVDE+GRTALLFVAGLGS+ CVR+LAEAGA+LD
Sbjct: 132 PWWTAARKADEQALSQLLED---RDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLD 191

Query: 259 HRDNSGGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAREILKATPR---V 318
           HRD  GG TALHMAAGYV+PE VE LVELGAD E+ED++G T L LAREILK TP+   +
Sbjct: 192 HRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPM 251

Query: 319 QFMRRLGLERVIGAVERVVYEYAEVEQLLEKRGKGENLEYLVKWKDGEDNEWVKVGLIAE 378
           QF RR+GLE+VI  +E  V+EYAEV++++EKRGKG+++EYLV+WKDG D EWVK   +AE
Sbjct: 252 QFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAE 311

Query: 379 DLVADFEAGLEYAVAEAVVGKRVGDDGK-MEYLVKWTDIQDATWEPQANVDPDLVNEFEK 430
           D+  D+E GLEYAVAE+V+GKRVGDDGK +EYLVKWTD+ DATWEPQ NVD  LV  +++
Sbjct: 312 DVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQ 369

[Close]

BLAST of CaUC02G029750 vs. TAIR 10
Match: AT4G35450.3 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 42/120 (35.00%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 197 AKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNS 256
           A   D   LK+ +  A G + D  D +GRTAL F  G G   C ++L +AGA+++  D +
Sbjct: 226 ASLGDVEGLKAAL--ASGGNKDEEDSEGRTALHFACGYGELKCAQVLIDAGASVNAVDKN 285

Query: 257 GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAR-----EILKATPRVQFM 312
              T LH AAGY + E V LL+E GA   +++   +TP+ +A+     E++K   +  F+
Sbjct: 286 KN-TPLHYAAGYGRKECVSLLLENGAAVTLQNLDEKTPIDVAKLNSQLEVVKLLEKDAFL 342

[Close]

BLAST of CaUC02G029750 vs. TAIR 10
Match: AT4G35450.4 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 42/120 (35.00%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 197 AKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNS 256
           A   D   LK+ +  A G + D  D +GRTAL F  G G   C ++L +AGA+++  D +
Sbjct: 188 ASLGDVEGLKAAL--ASGGNKDEEDSEGRTALHFACGYGELKCAQVLIDAGASVNAVDKN 247

Query: 257 GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAR-----EILKATPRVQFM 312
              T LH AAGY + E V LL+E GA   +++   +TP+ +A+     E++K   +  F+
Sbjct: 248 KN-TPLHYAAGYGRKECVSLLLENGAAVTLQNLDEKTPIDVAKLNSQLEVVKLLEKDAFL 304

[Close]

BLAST of CaUC02G029750 vs. TAIR 10
Match: AT4G35450.2 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 42/120 (35.00%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 197 AKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNS 256
           A   D   LK+ +  A G + D  D +GRTAL F  G G   C ++L +AGA+++  D +
Sbjct: 226 ASLGDVEGLKAAL--ASGGNKDEEDSEGRTALHFACGYGELKCAQVLIDAGASVNAVDKN 285

Query: 257 GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAR-----EILKATPRVQFM 312
              T LH AAGY + E V LL+E GA   +++   +TP+ +A+     E++K   +  F+
Sbjct: 286 KN-TPLHYAAGYGRKECVSLLLENGAAVTLQNLDEKTPIDVAKLNSQLEVVKLLEKDAFL 342

[Close]

BLAST of CaUC02G029750 vs. TAIR 10
Match: AT4G35450.1 (ankyrin repeat-containing protein 2 )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 42/120 (35.00%), Postives = 66/120 (55.00%), Query Frame = 0


Query: 197 AKKADESALKSMIDSADGRDFDAVDEDGRTALLFVAGLGSNPCVRMLAEAGANLDHRDNS 256
           A   D   LK+ +  A G + D  D +GRTAL F  G G   C ++L +AGA+++  D +
Sbjct: 226 ASLGDVEGLKAAL--ASGGNKDEEDSEGRTALHFACGYGELKCAQVLIDAGASVNAVDKN 285

Query: 257 GGFTALHMAAGYVKPETVELLVELGADPEIEDDKGRTPLVLAR-----EILKATPRVQFM 312
              T LH AAGY + E V LL+E GA   +++   +TP+ +A+     E++K   +  F+
Sbjct: 286 KN-TPLHYAAGYGRKECVSLLLENGAAVTLQNLDEKTPIDVAKLNSQLEVVKLLEKDAFL 342

The following BLAST results are available for this feature:

BLAST of CaUC02G029750 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888741.1	3.6e-193	93.07	signal recognition particle 43 kDa protein, chloroplastic [Benincasa hispida]	[more]
XP_008447899.1	6.1e-177	88.83	PREDICTED: signal recognition particle 43 kDa protein, chloroplastic [Cucumis me...	[more]
XP_004153574.1	1.2e-175	89.01	signal recognition particle 43 kDa protein, chloroplastic [Cucumis sativus]	[more]
XP_022928488.1	6.2e-169	76.36	signal recognition particle 43 kDa protein, chloroplastic-like [Cucurbita moscha...	[more]
XP_023529738.1	2.6e-167	82.93	signal recognition particle 43 kDa protein, chloroplastic-like [Cucurbita pepo s...	[more]

BLAST of CaUC02G029750 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22265	1.9e-112	60.77	Signal recognition particle 43 kDa protein, chloroplastic OS=Arabidopsis thalian...	[more]
Q8LSQ2	3.1e-91	50.27	Probable signal recognition particle 43 kDa protein, chloroplastic OS=Oryza sati...	[more]
Q06527	8.8e-09	38.58	Ankyrin homolog OS=Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 10...	[more]
Q9VL06	1.9e-08	34.29	E3 ubiquitin-protein ligase Ufd4 OS=Drosophila melanogaster OX=7227 GN=Ufd4 PE=2...	[more]
Q69ZR2	3.3e-08	32.04	E3 ubiquitin-protein ligase HECTD1 OS=Mus musculus OX=10090 GN=Hectd1 PE=1 SV=2	[more]

BLAST of CaUC02G029750 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BHX6	3.0e-177	88.83	signal recognition particle 43 kDa protein, chloroplastic OS=Cucumis melo OX=365...	[more]
A0A0A0K634	5.6e-176	89.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018780 PE=4 SV=1	[more]
A0A6J1EP65	3.0e-169	76.36	signal recognition particle 43 kDa protein, chloroplastic-like OS=Cucurbita mosc...	[more]
A0A6J1JM92	2.4e-166	76.70	signal recognition particle 43 kDa protein, chloroplastic OS=Cucurbita maxima OX...	[more]
A0A6J1C248	2.3e-153	77.30	signal recognition particle 43 kDa protein, chloroplastic OS=Momordica charantia...	[more]

BLAST of CaUC02G029750 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47450.1	1.3e-113	60.77	chloroplast signal recognition particle component (CAO)	[more]
AT4G35450.3	4.8e-10	35.00	ankyrin repeat-containing protein 2	[more]
AT4G35450.4	4.8e-10	35.00	ankyrin repeat-containing protein 2	[more]
AT4G35450.2	4.8e-10	35.00	ankyrin repeat-containing protein 2	[more]
AT4G35450.1	4.8e-10	35.00	ankyrin repeat-containing protein 2	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 188..219 e-value: 900.0 score: 5.3 coord: 257..286 e-value: 4.6E-4 score: 29.5 coord: 223..252 e-value: 7.3 score: 15.5
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 257..289 score: 11.99496
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 223..255 score: 10.47249
IPR000953	Chromo/chromo shadow domain	SMART	SM00298	chromo_7	coord: 380..430 e-value: 1.0E-6 score: 38.3 coord: 142..194 e-value: 2.4E-4 score: 30.4 coord: 329..379 e-value: 1.5E-6 score: 37.7
IPR000953	Chromo/chromo shadow domain	PROSITE	PS50013	CHROMO_2	coord: 381..437 score: 11.815401
IPR000953	Chromo/chromo shadow domain	PROSITE	PS50013	CHROMO_2	coord: 331..360 score: 8.865101
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 196..288 e-value: 2.8E-10 score: 40.6
IPR023780	Chromo domain	PFAM	PF00385	Chromo	coord: 383..427 e-value: 1.6E-7 score: 31.1
None	No IPR available	GENE3D	2.40.50.40		coord: 377..429 e-value: 8.0E-26 score: 91.4
None	No IPR available	GENE3D	2.40.50.40		coord: 327..376 e-value: 1.6E-17 score: 64.7 coord: 141..187 e-value: 1.9E-15 score: 58.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 122..145
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 122..137
None	No IPR available	PANTHER	PTHR24128	HOMEOBOX PROTEIN WARIAI	coord: 77..437
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 223..255 score: 9.118069
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 257..289 score: 10.630358
None	No IPR available	CDD	cd18628	CD3_cpSRP43_like	coord: 378..426 e-value: 3.8607E-11 score: 56.2356
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 188..326 e-value: 1.0E-35 score: 124.3
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	48403	Ankyrin repeat	coord: 189..300
IPR030300	Signal recognition particle 43kDa protein	PANTHER	PTHR24128:SF43	SIGNAL RECOGNITION PARTICLE 43 KDA PROTEIN, CHLOROPLASTIC	coord: 77..437
IPR016197	Chromo-like domain superfamily	SUPERFAMILY	54160	Chromo domain-like	coord: 329..376
IPR016197	Chromo-like domain superfamily	SUPERFAMILY	54160	Chromo domain-like	coord: 139..193
IPR016197	Chromo-like domain superfamily	SUPERFAMILY	54160	Chromo domain-like	coord: 379..427

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC02G029750.1	CaUC02G029750.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070208	protein heterotrimerization
biological_process	GO:0045038	protein import into chloroplast thylakoid membrane
biological_process	GO:0009644	response to high light intensity
biological_process	GO:0009416	response to light stimulus
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0080085	signal recognition particle, chloroplast targeting
molecular_function	GO:0097718	disordered domain specific binding
molecular_function	GO:0042802	identical protein binding
molecular_function	GO:0030674	protein-macromolecule adaptor activity
molecular_function	GO:0005515	protein binding