CaUC01G014190 (gene) Watermelon (USVL246-FR2) v1

Overview


Name	CaUC01G014190
Type	gene
Organism	Citrullus amarus (Watermelon (USVL246-FR2) v1)
Description	kunitz trypsin inhibitor 2
Location	Ciama_Chr01: 27212999 .. 27213895 (+)
RNA-Seq Expression	CaUC01G014190
Synteny	CaUC01G014190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGAAAATAATGCATCAAGAGAAAGAGAATAGTGAGCAATTTCGATTCTCTCTCTTTAATTCCCTATAAATACTGAACCTTTCCAAATCTAATTCATGCACCCACAAAAAGAAAAATCACATAAAAACCAATACGGAAAAAAGAAAATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTCGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCAGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGATTGAAATTGAAAGTTGAAATAAAGTTCTGAACTCGATCTCCATTGTGTGTGTAATAGTAATCAGTTTGTCAAATAATTTGTGTTAAATCATATCAGTGATTATCTAAATTAAATAAAAGATCTGGTTTTTTTTA

mRNA sequence

GGAAAATAATGCATCAAGAGAAAGAGAATAGTGAGCAATTTCGATTCTCTCTCTTTAATTCCCTATAAATACTGAACCTTTCCAAATCTAATTCATGCACCCACAAAAAGAAAAATCACATAAAAACCAATACGGAAAAAAGAAAATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTCGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCAGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGATTGAAATTGAAAGTTGAAATAAAGTTCTGAACTCGATCTCCATTGTGTGTGTAATAGTAATCAGTTTGTCAAATAATTTGTGTTAAATCATATCAGTGATTATCTAAATTAAATAAAAGATCTGGTTTTTTTTA

Coding sequence (CDS)

ATGCACCCACAAAAAGAAAAATCACATAAAAACCAATACGGAAAAAAGAAAATGAAGAATTTGGGAATATTACTTTCTTTTCTTTTCATTCTCCTTGCCTCTACCGTGGTCCGCTTCTCCAGAGCCGACGCTTCGCCGGAGGCCGTCCGCGACATCGACGGCGATAAGCTCCGAGCCGGCGTCAATTATTACATCCTCCCTGTTATCCGCGGCAGAGGCGGCGGCCTAACCCTAGGCAACCTCCAATCGGAGACATGTCCACTCAACGTGGTTCAAGAACAATTCGAAGTGATGAACGGCTTGCCTACAACATTCGCGCCTGTAAACCCTAAAAAGGGAGTGATTCGAGTTTCGACGGATTTGAACGTGCAATTCGAGGCAAGTACGATCTGCGCCACATCGACGGTGTGGAAATTGGACAAATTCGACGAATCGACTGGACAATGGTTCGTGACGATCGGCGGAAGCAGAGGAAATCCAGGGGTGGAGACGGTGGATAATTGGTTCAAAATTGAGAAGCACGGTCAGGATTACAAGTTGGTGTTCTGTCCGAGTGTATGCGATTTCTGTAAAGTGATGTGTAGAGATATTGGAATCTTCTTCAAGAATGGAAAGAGGGCTTTGGCTTTGAGCGATACGCCATTCCCAGTTATGTTCAAGAAAGTTTGA

Protein sequence

MHPQKEKSHKNQYGKKKMKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKNGKRALALSDTPFPVMFKKV

Homology

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BLAST of CaUC01G014190 vs. NCBI nr
Match: XP_038895203.1 (kunitz trypsin inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 400.6 bits (1028), Expect = 8.9e-108
Identity = 192/205 (93.66%), Postives = 196/205 (95.61%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 77
           MKN GILLSFLFILLAST VRFSRADASPEAVRDIDG KLRAGVNYYILPVIRGRGGGLT
Sbjct: 1   MKNFGILLSFLFILLASTEVRFSRADASPEAVRDIDGKKLRAGVNYYILPVIRGRGGGLT 60

Query: 78  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 137
           L NLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LSNLQSENCPVNVVQEQFELMNGFPTTFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 138 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDI 197
           KLDK DESTGQWFVTIGGSRGNPGVETVDNWFKI KHG+DYKLVFCPSVCDFCKVMCRDI
Sbjct: 121 KLDKVDESTGQWFVTIGGSRGNPGVETVDNWFKIVKHGKDYKLVFCPSVCDFCKVMCRDI 180

Query: 198 GIFFKNGKRALALSDTPFPVMFKKV 223
           GIFFKNGKRALALSDTPFPVMFKKV
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of CaUC01G014190 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 384.8 bits (987), Expect = 5.0e-103
Identity = 185/206 (89.81%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLAST-VVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 77
           MKN GIL  FLFILLAST ++RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 78  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 137
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 138 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRD 197
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 198 IGIFFKNGKRALALSDTPFPVMFKKV 223
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of CaUC01G014190 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 381.7 bits (979), Expect = 4.3e-102
Identity = 182/204 (89.22%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 19  KNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTL 78
           KN GI   F+FILLAST +RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 79  GNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 138
           GNLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 139 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIG 198
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 199 IFFKNGKRALALSDTPFPVMFKKV 223
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of CaUC01G014190 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.2 bits (949), Expect = 1.3e-98
Identity = 178/205 (86.83%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 77
           MKN   LLSFLFILLAS  VR SRADASP+AVRDIDG KLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 78  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 137
           LGNLQS+ CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 138 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDI 197
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 198 GIFFKNGKRALALSDTPFPVMFKKV 223
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

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BLAST of CaUC01G014190 vs. NCBI nr
Match: XP_023520252.1 (kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.0 bits (946), Expect = 2.9e-98
Identity = 178/205 (86.83%), Postives = 187/205 (91.22%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 77
           MKN   LLSFLFILLAS  VR SRADASP+AVRDIDG KLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 78  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 137
           LGNLQS+ CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 138 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDI 197
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGARGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 198 GIFFKNGKRALALSDTPFPVMFKKV 223
           GIFFKNGKRALALSDTPFPVMFK V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKVV 203

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BLAST of CaUC01G014190 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 5.5e-60
Identity = 109/198 (55.05%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 25  LSFLFILLASTVV-RFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 84
           L ++F+LLA  +  R    +A+ E V+DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 85  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 144
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 145 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 204
           E+T QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 205 GKRALALSDTPFPVMFKK 222
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

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BLAST of CaUC01G014190 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 215.7 bits (548), Expect = 5.3e-55
Identity = 116/216 (53.70%), Postives = 143/216 (66.20%), Query Frame = 0


Query: 18  MKNLGIL-LSFLFI---LLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRG 77
           MK L +L LSF F+   L A+     S AD++P  V DIDG+KLR G NYYI+PV+R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 78  GGLTLGNLQSE---TCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTI 137
           GGLT+          CP  VVQ + EV +  P  F P NPK+ V+RVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 138 C--ATSTVWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKH--GQDYKLVFCPSV 197
           C   +STVW+LDK+DESTGQ+FVTIGG +GNPG ET+ +WFKIE+      YKLVFCP+V
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 198 CDFCKVMCRDIGIFF-KNGKRALALSDTPFPVMFKK 222
           C  CKV C D+GI+  + G+R LALSD PF   F K
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of CaUC01G014190 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 3.6e-43
Identity = 95/204 (46.57%), Postives = 125/204 (61.27%), Query Frame = 0


Query: 23  ILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGR-GGGLTLGNL 82
           +LL F F    S    F  A+A+   V D DGD+L+ GV YY+L  I G  GGGL LG  
Sbjct: 8   VLLLFAF---TSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRA 67

Query: 83  QSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE--ASTICATSTVWKL 142
             ++CP  VVQ + ++ NG P  F+  + K  V+RVSTD+N++F      +C+TSTVW+L
Sbjct: 68  TGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRL 127

Query: 143 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG-QDYKLVFCPSVCDFCKVMCRDIG 202
           D +D S G+W+VT  G +G PG  T+ +WFKIEK G   YK  FCPSVCD C  +C DIG
Sbjct: 128 DNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIG 187

Query: 203 IFF-KNGKRALALSDTPFPVMFKK 222
                +G+  LALSD  +  MFKK
Sbjct: 188 RHSDDDGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of CaUC01G014190 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 6.5e-37
Identity = 88/196 (44.90%), Postives = 117/196 (59.69%), Query Frame = 0


Query: 29  FILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 88
           +++LA T V  S A     AV DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 89  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 148
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 149 QWFVTIGGSRGNPGVETVDNWFKIEKHGQD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 208
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 209 RALALSDTPFPVMFKK 222
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of CaUC01G014190 vs. ExPASy Swiss-Prot
Match: Q41015 (Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum OX=3888 GN=PIP20-1 PE=1 SV=2)

HSP 1 Score: 115.9 bits (289), Expect = 5.7e-25
Identity = 74/198 (37.37%), Postives = 101/198 (51.01%), Query Frame = 0


Query: 27  FLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRG-RGGGLTLGNLQSET 86
           FLF+ + +  + FS  D   E V D +G+ +  GV Y+ILP IRG  GGG+ LG     T
Sbjct: 12  FLFVFITNLSLAFSNEDV--EQVLDFNGNPIFPGVQYFILPAIRGPAGGGVRLGRTGDLT 71

Query: 87  CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFDES 146
           CP+ V+Q++ EV NGLP  F       G+I   T + +++     CA S+ W L   D  
Sbjct: 72  CPVTVLQDRQEVKNGLPVKFVIPEISPGIIFTGTPIEIEYTKKPNCAKSSKW-LVFVDNV 131

Query: 147 TGQWFVTIGGSRGNPGVETVDNWFKIEKH--GQDYKLVFCPSVCDFCKVMCRDIGIF--F 206
             +  V IGG    PGV+T+   FKIEKH  G  YKL FC          C D+G F   
Sbjct: 132 IQKACVGIGGPENYPGVQTLSGLFKIEKHESGFGYKLGFCVK----GSPTCLDVGRFDND 191

Query: 207 KNGKRALALSDTPFPVMF 220
           ++G+R        F V+F
Sbjct: 192 EDGRRLNLTEHESFQVVF 202

[Close]

BLAST of CaUC01G014190 vs. ExPASy TrEMBL
Match: A0A0A0LTW2 (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.4e-103
Identity = 185/206 (89.81%), Postives = 192/206 (93.20%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLAST-VVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGL 77
           MKN GIL  FLFILLAST ++RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 78  TLGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTV 137
           TLGNLQSE CPLNVVQEQ EVMNG PTTF PVNPKKGV+RVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 138 WKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRD 197
           WKLDKFDESTGQW VTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 198 IGIFFKNGKRALALSDTPFPVMFKKV 223
           IGIFFKNG+RALALSDTPFPVMFKKV
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of CaUC01G014190 vs. ExPASy TrEMBL
Match: A0A5D3BIG0 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002770 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.1e-102
Identity = 182/204 (89.22%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 19  KNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTL 78
           KN GI   F+FILLAST +RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 79  GNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 138
           GNLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 139 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIG 198
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 199 IFFKNGKRALALSDTPFPVMFKKV 223
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of CaUC01G014190 vs. ExPASy TrEMBL
Match: A0A1S3ASR3 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.1e-102
Identity = 182/204 (89.22%), Postives = 191/204 (93.63%), Query Frame = 0


Query: 19  KNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTL 78
           KN GI   F+FILLAST +RFS ADASPEAV DIDG KLRAGVNYYILPV RGRGGGLTL
Sbjct: 3   KNFGIFY-FIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTL 62

Query: 79  GNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWK 138
           GNLQSE CP+NVVQEQFE+MNG PTTF PVNPKKGV+RVSTDLNVQF+ASTIC TSTVWK
Sbjct: 63  GNLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWK 122

Query: 139 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIG 198
           LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHG+DYKLVFCP+VC+FCKVMCRDIG
Sbjct: 123 LDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIG 182

Query: 199 IFFKNGKRALALSDTPFPVMFKKV 223
           IFFKNGKRALALSDTPFPVMFKKV
Sbjct: 183 IFFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of CaUC01G014190 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.2e-99
Identity = 178/205 (86.83%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 77
           MKN   LLSFLFILLAS  VR SRADASP+AVRDIDG KLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 78  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 137
           LGNLQS+ CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 138 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDI 197
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 198 GIFFKNGKRALALSDTPFPVMFKKV 223
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of CaUC01G014190 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 6.2e-99
Identity = 178/205 (86.83%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 18  MKNLGILLSFLFILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLT 77
           MKN   LLSFLFILLAS  VR SRADASP+AVRDIDG KLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFA-LLSFLFILLASE-VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60

Query: 78  LGNLQSETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVW 137
           LGNLQS+ CPLNVVQEQFE+MNGLP  F PVNPKKGV+RVSTDLNVQFEASTICATSTVW
Sbjct: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 138 KLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDI 197
           KLDKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180

Query: 198 GIFFKNGKRALALSDTPFPVMFKKV 223
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of CaUC01G014190 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 232.3 bits (591), Expect = 3.9e-61
Identity = 109/198 (55.05%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 25  LSFLFILLASTVV-RFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQS 84
           L ++F+LLA  +  R    +A+ E V+DI+G  L  GVNYYILPVIRGRGGGLT+ NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 85  ETCPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEASTICATSTVWKLDKFD 144
           ETCP +V+Q+QFEV  GLP  F+P + K   I VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 145 ESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCRDIGIFFKN 204
           E+T QWF++  G  GNPG +TVDNWFKI+K  +DYK+ FCP+VC+FCKV+CRD+G+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 205 GKRALALSDTPFPVMFKK 222
           GKR LALSD P  VMFK+
Sbjct: 184 GKRRLALSDVPLKVMFKR 194

[Close]

BLAST of CaUC01G014190 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 155.6 bits (392), Expect = 4.6e-38
Identity = 88/196 (44.90%), Postives = 117/196 (59.69%), Query Frame = 0


Query: 29  FILLASTVVRFSRADASPEAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGNLQSETCPL 88
           +++LA T V  S A     AV DIDG+ +    +YY+LPVIRGRGGGLTL     + CP 
Sbjct: 13  YLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPY 72

Query: 89  NVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFE-ASTICATSTVWKLDKFDESTG 148
           ++VQE  EV  G+P  F+    K   +  S +LN++ +  +TIC  ST W++ +FD    
Sbjct: 73  DIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERK 132

Query: 149 QWFVTIGGSRGNPGVETVDNWFKIEKHGQD-YKLVFCPSVCDFCKVMCRDIGIFFKN-GK 208
           Q+FV  G      G +++ ++FKIEK G+D YK VFCP  CD     C D+GIF    G 
Sbjct: 133 QYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGV 192

Query: 209 RALALSDTPFPVMFKK 222
           R LALSD PF VMFKK
Sbjct: 193 RRLALSDKPFLVMFKK 204

[Close]

BLAST of CaUC01G014190 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 108.6 bits (270), Expect = 6.5e-24
Identity = 72/208 (34.62%), Postives = 115/208 (55.29%), Query Frame = 0


Query: 23  ILLSFLFILLASTV-VRFSRADASP-EAVRDIDGDKLRAGVNYYILPVIRGRGGGLTLGN 82
           + LSF+ + + S +    S ADA+P + V DI G  +++ V YYI+P   G GGGL   N
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 83  LQSET----CPLNVVQEQFEVMNGLPTTFAPVNPKKGVIRVSTDLNVQFEAST-ICATST 142
               T      L++VQ     ++GLP TF+P+N K   +++S  LN++F+++  +C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 143 VWKLDKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGQDYKLVFCPSVCDFCKVMCR 202
           VW++D       + FV+IGG +G       ++WF+I++ G  YKL++CP       V C 
Sbjct: 124 VWRID-HSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACI 183

Query: 203 DIGI-FFKNGKRALALS-DTPFPVMFKK 222
           ++ +    +G R L LS D  F V F+K
Sbjct: 184 NVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of CaUC01G014190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038895203.1	8.9e-108	93.66	kunitz trypsin inhibitor 5-like [Benincasa hispida]	[more]
XP_004137534.1	5.0e-103	89.81	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]
XP_008437058.1	4.3e-102	89.22	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]
XP_022923703.1	1.3e-98	86.83	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_023520252.1	2.9e-98	86.83	kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CaUC01G014190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	5.5e-60	55.05	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	5.3e-55	53.70	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	3.6e-43	46.57	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	6.5e-37	44.90	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
Q41015	5.7e-25	37.37	Kunitz-type trypsin inhibitor-like 1 protein OS=Pisum sativum OX=3888 GN=PIP20-1...	[more]

BLAST of CaUC01G014190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LTW2	2.4e-103	89.81	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1	[more]
A0A5D3BIG0	2.1e-102	89.22	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00277...	[more]
A0A1S3ASR3	2.1e-102	89.22	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1	[more]
A0A6J1KRM6	6.2e-99	86.83	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	6.2e-99	86.83	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]

BLAST of CaUC01G014190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	3.9e-61	55.05	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	4.6e-38	44.90	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	6.5e-24	34.62	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 86..106 score: 34.45 coord: 48..77 score: 44.51 coord: 168..187 score: 25.39 coord: 191..220 score: 25.03
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 48..222 e-value: 5.2E-75 score: 265.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 49..221 e-value: 1.1E-60 score: 204.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 25..222
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 49..65
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 48..221 e-value: 1.2982E-59 score: 183.312
None	No IPR available	GENE3D	2.80.10.50		coord: 43..222 e-value: 1.7E-77 score: 261.1
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 25..222
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 45..222

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CaUC01G014190.1	CaUC01G014190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity