CaUC01G009550 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC01G009550
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionPeptidase A1 domain-containing protein
LocationCiama_Chr01: 11932070 .. 11933602 (+)
RNA-Seq ExpressionCaUC01G009550
SyntenyCaUC01G009550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTTCAATATCAACCTCCATTGCCACAAAATTCTTGAGCTTTTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTGCAAACCCTAAAACCAATTTTCCCACAGATTCTCTAGTTCTTGGCCTTGTTCATTCAAGAACATCCCTCCTTATCCCTAAAAAAGGCTATAATTCCATTTCAAGGAAGAGACTGAAGACAATGGAAATGGGTAGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGGAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTTAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGAAACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGGCAATTGCCCTAGGCCATGCCCTTCCTTTGCTTACACTTATGGGGCGAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTCATGCATGGAAATAATATTAATTCTCCAAATTCCACTAAGCAAATCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGTAGAGGTTTACTTTCTCTTCCTTTCCAATTAGGGTTTTCTCATAAGGGATTTTCTCATTGTTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAACCTTGCTATTTCTTCAAAAGATGACCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAATCAATCACTATAGGAAACGGAAATAATAATTTTAGATTTGGTGTTTCTTTCAAATTGAGAGAGATTGATACAAAGGGTAATGGGGGTATGTTGATTGATTCTGGTACTACTTACACTCATTTACCTGAACCTTTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAGCCTATCCAAGAGCCAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAGAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCACAAGGAAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAACCATGGACGGTGTCGGTGGCGATAACGACGACAGCGACGGGCCGGCTGGCATTTTTGGAAGCTTCCAACAACAAAATTTAGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTCTGTTGCTGCCACTCAAGGACTCCACAAGAATGTTTGA

mRNA sequence

ATGCCTTCAATATCAACCTCCATTGCCACAAAATTCTTGAGCTTTTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTGCAAACCCTAAAACCAATTTTCCCACAGATTCTCTAGTTCTTGGCCTTGTTCATTCAAGAACATCCCTCCTTATCCCTAAAAAAGGCTATAATTCCATTTCAAGGAAGAGACTGAAGACAATGGAAATGGGTAGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGGAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTTAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGAAACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGGCAATTGCCCTAGGCCATGCCCTTCCTTTGCTTACACTTATGGGGCGAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTCATGCATGGAAATAATATTAATTCTCCAAATTCCACTAAGCAAATCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGTAGAGGTTTACTTTCTCTTCCTTTCCAATTAGGGTTTTCTCATAAGGGATTTTCTCATTGTTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAACCTTGCTATTTCTTCAAAAGATGACCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAATCAATCACTATAGGAAACGGAAATAATAATTTTAGATTTGGTGTTTCTTTCAAATTGAGAGAGATTGATACAAAGGGTAATGGGGGTATGTTGATTGATTCTGGTACTACTTACACTCATTTACCTGAACCTTTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAGCCTATCCAAGAGCCAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAGAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCACAAGGAAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAACCATGGACGGTGTCGGTGGCGATAACGACGACAGCGACGGGCCGGCTGGCATTTTTGGAAGCTTCCAACAACAAAATTTAGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTCTGTTGCTGCCACTCAAGGACTCCACAAGAATGTTTGA

Coding sequence (CDS)

ATGCCTTCAATATCAACCTCCATTGCCACAAAATTCTTGAGCTTTTTCCTTCTTCTTGTATATGTCTCAAGAAAAACCCTAGCTGCAAACCCTAAAACCAATTTTCCCACAGATTCTCTAGTTCTTGGCCTTGTTCATTCAAGAACATCCCTCCTTATCCCTAAAAAAGGCTATAATTCCATTTCAAGGAAGAGACTGAAGACAATGGAAATGGGTAGTGATGATAATGTGATAGAGCCATTGAGAGAAATTAGGGATGGTTATTTGATGTCCTTAACATTAGGGACACCCCCACAAGTTATTCAAGTGTATATGGACACTGGAAGTGATCTCACATGGGTTCCTTGTGGGAACCTCTCTTTTGATTGTCAAGATTGTGAGGAGTATCAAAACAATGTTTTAGGCCCAAAATTGGCTGCTTTTTTGCCTACTCATTCTTCTACTTCTATTAGAGAAACTTGTGGTAGCTCCTTTTGTATGGATATTCATAGCTCTGATAACCCTTTTGATCCTTGCACAATTGCTGGTTGTTCCCTTGCTACCCTTGTGAAGGGCAATTGCCCTAGGCCATGCCCTTCCTTTGCTTACACTTATGGGGCGAGTGGGGTTGTAATTGGAACTTTAACAAGAGATGTCCTTTTCATGCATGGAAATAATATTAATTCTCCAAATTCCACTAAGCAAATCCCTAGGTTTTGTTTTGGATGTGTTGGTGCAAGTTATAGAGAGCCAATTGGGATTGCTGGTTTTGGTAGAGGTTTACTTTCTCTTCCTTTCCAATTAGGGTTTTCTCATAAGGGATTTTCTCATTGTTTCTTGCCTTTTAAATTCTCAAATAACCCTAATTTCTCAAGCCCTTTGATTCTTGGTAACCTTGCTATTTCTTCAAAAGATGACCATTTGCAATTTACTCCTTTGTTGAAAAGTCCAATTTACCCCAACTACTACTATATTGGGCTTGAATCAATCACTATAGGAAACGGAAATAATAATTTTAGATTTGGTGTTTCTTTCAAATTGAGAGAGATTGATACAAAGGGTAATGGGGGTATGTTGATTGATTCTGGTACTACTTACACTCATTTACCTGAACCTTTGTATTCACAACTTATTTCAAATCTTGAGTCAGTGATAGCCTATCCAAGAGCCAAACAAGTTGAACTCAATACTGGATTTGATCTTTGTTATAAAGTTCCTTGTAGAAACAACAATTTTTCTTTTATTGATGACTCTCAACTCCCTTCTATAACATTCCATTTTTTGAATAATGTTAGTGTTGTTTTGCCACAAGGAAATAACTTCTATGCCATGGCTGCTCCAATTAACTCCACTGTGGTTAAATGCTTGTTGTTTCAAACCATGGACGGTGTCGGTGGCGATAACGACGACAGCGACGGGCCGGCTGGCATTTTTGGAAGCTTCCAACAACAAAATTTAGAGGTTGTTTATGACTTGGAGAAGGAAAGATTAGGGTTTCAACCAATGGATTGTGCTTCTGTTGCTGCCACTCAAGGACTCCACAAGAATGTTTGA

Protein sequence

MPSISTSIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNSISRKRLKTMEMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCASVAATQGLHKNV
Homology
BLAST of CaUC01G009550 vs. NCBI nr
Match: XP_038893627.1 (probable aspartyl protease At4g16563 [Benincasa hispida])

HSP 1 Score: 971.5 bits (2510), Expect = 2.9e-279
Identity = 475/512 (92.77%), Postives = 488/512 (95.31%), Query Frame = 0

Query: 1   MPSISTSIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNS 60
           M SISTS A K LS+FLLLVYVSRKTLA NPKTN P DSLV+GLVHSRT+LL PKKGYN 
Sbjct: 1   MASISTSFAKKILSYFLLLVYVSRKTLATNPKTNGPKDSLVIGLVHSRTTLLTPKKGYNF 60

Query: 61  ISRKRLKTMEMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS 120
           ISRKR+K MEM  DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS
Sbjct: 61  ISRKRMKAMEM--DDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS 120

Query: 121 FDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLA 180
           FDCQDCEEYQNNV GPKLAAFLPTHSSTSIR+TCGSSFCMDIHSSDNPFDPCTIAGCSLA
Sbjct: 121 FDCQDCEEYQNNVSGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCSLA 180

Query: 181 TLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPN-STKQIPRFCFGCVGA 240
           TLVK  CPRPCPSFAYTYGASGVVIGTLTRDVL MH NNINSPN STK+ PRFCFGCVGA
Sbjct: 181 TLVKATCPRPCPSFAYTYGASGVVIGTLTRDVLLMHINNINSPNSSTKKTPRFCFGCVGA 240

Query: 241 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDD 300
           SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLA+SSKD+
Sbjct: 241 SYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAVSSKDE 300

Query: 301 HLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTY 360
           HLQFTPLLKSPIYPNYYYIGLESITIGNGN+NFRFGVSF LREIDTKGNGGMLIDSGTTY
Sbjct: 301 HLQFTPLLKSPIYPNYYYIGLESITIGNGNSNFRFGVSFNLREIDTKGNGGMLIDSGTTY 360

Query: 361 THLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFH 420
           THLPEPLYSQLISNLESVI+YPRAKQVELNTGFDLCYKVPC+NNNFSFIDDSQLPSITFH
Sbjct: 361 THLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNFSFIDDSQLPSITFH 420

Query: 421 FLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGD-NDDSDGPAGIFGSFQQQN 480
           FLNNVSVVLPQ NNFYAMAAPINSTVVKCLLFQ+MDGVGGD +DD DGPAGIFGSFQQQN
Sbjct: 421 FLNNVSVVLPQENNFYAMAAPINSTVVKCLLFQSMDGVGGDTDDDRDGPAGIFGSFQQQN 480

Query: 481 LEVVYDLEKERLGFQPMDCASVAATQGLHKNV 511
           LEVVYDLEKERLGFQPMDCA VAATQGLHKNV
Sbjct: 481 LEVVYDLEKERLGFQPMDCAYVAATQGLHKNV 510

BLAST of CaUC01G009550 vs. NCBI nr
Match: XP_008459091.1 (PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa] >TYK29371.1 aspartic proteinase nepenthesin-2 [Cucumis melo var. makuwa])

HSP 1 Score: 951.8 bits (2459), Expect = 2.4e-273
Identity = 462/517 (89.36%), Postives = 488/517 (94.39%), Query Frame = 0

Query: 1   MPSI-STSIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYN 60
           MPSI STSIATKFLS FLLLV+ S++TLA NPKTNFP DSLVLGLVHSRTSLL PKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  SISRKRLKTM-EMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
            IS+KR+K M +M  DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+ GPKLAAFLPTHSSTSIR+TCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHG----NNINSPNSTKQIPRFCF 240
           LATLVKG CPRPCPSFAYTYGASGVV G+LTRDVLFMHG    NN N+ N+ KQ+PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGA+YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKD++LQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLP 420
           SGTTYTHLPEPLYSQLISNLESVI+YPRAKQVELNTGFDLCYKVPC+NNN SF+DDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDND-DSDGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLL+Q+MDGVG DND D +GPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLEVVYDLEKERLGFQPMDCASVAATQGLHKNV 511
           FQQQNL+VVYDLEKERLGFQ MDC SVAA QGLHKNV
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of CaUC01G009550 vs. NCBI nr
Match: XP_004145478.2 (probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical protein Csa_007266 [Cucumis sativus])

HSP 1 Score: 945.7 bits (2443), Expect = 1.7e-271
Identity = 456/513 (88.89%), Postives = 484/513 (94.35%), Query Frame = 0

Query: 1   MPSIST-SIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYN 60
           MPSIS+ S ATKFLS FLLLV+VS +TLA NPKTNFP DSLVLGLVHSRTSLL PKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  SISRKRLKTMEM-GSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
            IS+KR+K M+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+ GP+LAAFLPTHSSTSIR+TCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVG 240
           LA+LVKG CPRPCPSFAYTYGASGVV G+LTRDVLF HGN  N+ N+ KQIPRFCFGCVG
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240

Query: 241 ASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300
           A+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD
Sbjct: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300

Query: 301 DHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360
           ++LQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLIDSGTT
Sbjct: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360

Query: 361 YTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITF 420
           YTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPC+NNN SF+DD+QLPSITF
Sbjct: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420

Query: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDND-DSDGPAGIFGSFQQQ 480
           HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLL+Q+MDGVG DND D +GPAGIFGSFQQQ
Sbjct: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480

Query: 481 NLEVVYDLEKERLGFQPMDCASVAATQGLHKNV 511
           N+EVVYDLEKERLGFQPMDC SVAA QGLHKNV
Sbjct: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNV 513

BLAST of CaUC01G009550 vs. NCBI nr
Match: XP_023520027.1 (probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 863.2 bits (2229), Expect = 1.1e-246
Identity = 423/508 (83.27%), Postives = 462/508 (90.94%), Query Frame = 0

Query: 4   ISTSIATKFLSFFLLLVYVSRKTLA---ANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNS 63
           +++ +A  F    L+LV VS + +    ANPKT F  DSLVLGLVHSRTSLL PK+GYNS
Sbjct: 1   MASIVAKSFFVLVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRGYNS 60

Query: 64  ISRKRLKTMEMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS 123
           +SRKR+K MEMG+DD VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS
Sbjct: 61  LSRKRIKPMEMGNDD-VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLS 120

Query: 124 FDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLA 183
           FDCQDC+EYQNNVLGPKLAAFLPTHSSTSIR+TCGSSFC+DIHSSDNPFDPCTIAGCSLA
Sbjct: 121 FDCQDCDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCSLA 180

Query: 184 TLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGAS 243
           TLVKG CPRPCPSF+YTYGASG+VIGTLT+DV+F+HG   NSPNS+++IP+FCFGCVGA+
Sbjct: 181 TLVKGTCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG---NSPNSSRKIPKFCFGCVGAT 240

Query: 244 YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDH 303
           YREPIGIAGFGRGLLSLP+QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK +H
Sbjct: 241 YREPIGIAGFGRGLLSLPYQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK-EH 300

Query: 304 LQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYT 363
           L+FTPLLKSP YPNYYYIGLESITIGNG N  RFGVS +LREIDTKGNGG+LIDSGTTYT
Sbjct: 301 LKFTPLLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTYT 360

Query: 364 HLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHF 423
           HLPEPLYSQLISNLES+I+YPRAK+ ELNTGFDLCYKVP +NN F F D+ +LPSITFHF
Sbjct: 361 HLPEPLYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSITFHF 420

Query: 424 LNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLE 483
           LNNVSVVLPQGN+FYAMAAP NSTVVKCLLFQ+MDG      D DGPAGIFGSFQQQNLE
Sbjct: 421 LNNVSVVLPQGNSFYAMAAPSNSTVVKCLLFQSMDG------DGDGPAGIFGSFQQQNLE 480

Query: 484 VVYDLEKERLGFQPMDCASVAATQGLHK 509
           VVYDLEKERLGF+ MDCASVA +QGLHK
Sbjct: 481 VVYDLEKERLGFEGMDCASVAVSQGLHK 496

BLAST of CaUC01G009550 vs. NCBI nr
Match: KAG6583807.1 (putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.6e-245
Identity = 421/499 (84.37%), Postives = 456/499 (91.38%), Query Frame = 0

Query: 13  LSFFLLLVYVSRKTLA---ANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNSISRKRLKTM 72
           L   L+LV VS + +    ANPKT F  DSLVLGLVHSRTSLL PK+GYNS+SRKR+K M
Sbjct: 12  LVLVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRGYNSLSRKRIKPM 71

Query: 73  EMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEY 132
           EMG+DD VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDC+EY
Sbjct: 72  EMGNDD-VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCDEY 131

Query: 133 QNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNCPR 192
           QNNVLGPKLAAFLPTHSSTSIR+TCGSSFC+DIHSSDNPFDPCTIAGCSLATLVKG CPR
Sbjct: 132 QNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCSLATLVKGTCPR 191

Query: 193 PCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPIGIAG 252
           PCPSF+YTYGASG+VIGTLT+DV+F+HG   NSPNS+++IP+FCFGCVGA+YREPIGIAG
Sbjct: 192 PCPSFSYTYGASGLVIGTLTKDVIFIHG---NSPNSSRKIPKFCFGCVGATYREPIGIAG 251

Query: 253 FGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDHLQFTPLLKS 312
           FGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK +HL+FTPLLKS
Sbjct: 252 FGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK-EHLKFTPLLKS 311

Query: 313 PIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQ 372
           P YPNYYYIGLESITIGNG N  RFGVS +LREIDTKGNGG+LIDSGTTYTHLPEPLYSQ
Sbjct: 312 PFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTYTHLPEPLYSQ 371

Query: 373 LISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVSVVLP 432
           +ISNLES+I+YPRAK+ ELNTGFDLCYKVP +NN F F D+ +LPSITFHFLNNVSVVLP
Sbjct: 372 IISNLESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSITFHFLNNVSVVLP 431

Query: 433 QGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDLEKER 492
           QGN+FYAMAAP NSTVVKCLLFQ+MDG      D DGPAGIFGSFQQQNLEVVYDLEKER
Sbjct: 432 QGNSFYAMAAPSNSTVVKCLLFQSMDG------DGDGPAGIFGSFQQQNLEVVYDLEKER 491

Query: 493 LGFQPMDCASVAATQGLHK 509
           LGF+ MDCASVA +QGLHK
Sbjct: 492 LGFEGMDCASVAVSQGLHK 498

BLAST of CaUC01G009550 vs. ExPASy Swiss-Prot
Match: Q940R4 (Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g16563 PE=2 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 3.8e-56
Identity = 154/447 (34.45%), Postives = 217/447 (48.55%), Query Frame = 0

Query: 88  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVLGPKLAAFLPTHSS 147
           YL+SL++G+    + +Y+DTGSDL W PC    F C  CE   +  L P   + L   SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCE---SKPLPPSPPSSL---SS 142

Query: 148 TSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNC---PRPCPSFAYTYGASGVV 207
           ++   +C S  C   HSS    D C I+ C L  +  G+C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 208 IGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGF- 267
           +  L  D L +   ++++         F FGC   +  EPIG+AGFGRG LSLP QL   
Sbjct: 203 VAKLYSDSLSLPSVSVSN---------FTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVH 262

Query: 268 -SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------ISSKDDH------------L 327
             H G  FS+C +   F S+     SPLILG         + + DDH             
Sbjct: 263 SPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEF 322

Query: 328 QFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTH 387
            FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGTT+T 
Sbjct: 323 VFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTFTM 382

Query: 388 LPEPLYSQLISNLESVI--AYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFH 447
           LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P++  H
Sbjct: 383 LPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALVLH 442

Query: 448 FL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLFQTMDGVGGDNDDSDGPAG-IFGS 501
           F  N  SV LP+ N FY              + CL+       GGD  +  G  G I G+
Sbjct: 443 FAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAILGN 494

BLAST of CaUC01G009550 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.6e-33
Identity = 116/416 (27.88%), Postives = 165/416 (39.66%), Query Frame = 0

Query: 88  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVLGPKLAAFLPTHSS 147
           YLM++ +GTP       MDTGSDL W  C      C  C      +       F P  SS
Sbjct: 96  YLMNVAIGTPDSSFSAIMDTGSDLIWTQCE----PCTQCFSQPTPI-------FNPQDSS 155

Query: 148 TSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNCPRPCPSFAYTYGASGVVIGT 207
           +     C S +C D+     P + C    C                + Y YG      G 
Sbjct: 156 SFSTLPCESQYCQDL-----PSETCNNNEC---------------QYTYGYGDGSTTQGY 215

Query: 208 LTRDVLFMHGNNINSPNSTKQIPRFCFGC----VGASYREPIGIAGFGRGLLSLPFQLGF 267
           +  +              T  +P   FGC     G       G+ G G G LSLP QLG 
Sbjct: 216 MATETFTF---------ETSSVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGV 275

Query: 268 SHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDHLQFTPLLKSPIYPNYYYIGLESI 327
               FS+C   +  S+     S L LG+ A S   +    T L+ S + P YYYI L+ I
Sbjct: 276 GQ--FSYCMTSYGSSS----PSTLALGS-AASGVPEGSPSTTLIHSSLNPTYYYITLQGI 335

Query: 328 TIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVIAYPRA 387
           T+G  N     G+     ++   G GGM+IDSGTT T+LP+  Y+ +       I  P  
Sbjct: 336 TVGGDN----LGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTV 395

Query: 388 KQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINS 447
              E ++G   C++ P   +        Q+P I+  F   V  +  Q      + +P   
Sbjct: 396 D--ESSSGLSTCFQQPSDGSTV------QVPEISMQFDGGVLNLGEQN----ILISPAEG 437

Query: 448 TVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCAS 500
            +  CL   +   +G           IFG+ QQQ  +V+YDL+   + F P  C +
Sbjct: 456 VI--CLAMGSSSQLG---------ISIFGNIQQQETQVLYDLQNLAVSFVPTQCGA 437

BLAST of CaUC01G009550 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.4e-33
Identity = 124/438 (28.31%), Postives = 185/438 (42.24%), Query Frame = 0

Query: 72  GSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQN 131
           G   +V+  L +    Y   L +GTP + + + +DTGSD+ W+ C      C+ C    +
Sbjct: 126 GFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCA----PCRRCYSQSD 185

Query: 132 NVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNCPRPC 191
            +       F P  S T     C S  C  + S          AGC        N  R  
Sbjct: 186 PI-------FDPRKSKTYATIPCSSPHCRRLDS----------AGC--------NTRRKT 245

Query: 192 PSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGC--------VGASYRE 251
             +  +YG     +G  + + L    N +              GC        VGA+   
Sbjct: 246 CLYQVSYGDGSFTVGDFSTETLTFRRNRVKG---------VALGCGHDNEGLFVGAA--- 305

Query: 252 PIGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDHL 311
             G+ G G+G LS P Q G  F+ K FS+C +    S+ P   S ++ GN A+S      
Sbjct: 306 --GLLGLGKGKLSFPGQTGHRFNQK-FSYCLVDRSASSKP---SSVVFGNAAVSR---IA 365

Query: 312 QFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTH 371
           +FTPLL +P    +YY+GL  I++G        GV+  L ++D  GNGG++IDSGT+ T 
Sbjct: 366 RFTPLLSNPKLDTFYYVGLLGISVGGTRVP---GVTASLFKLDQIGNGGVIIDSGTSVTR 425

Query: 372 LPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFL 431
           L  P Y  +       +     K+    + FD C+       + S +++ ++P++  HF 
Sbjct: 426 LIRPAYIAMRDAFR--VGAKTLKRAPDFSLFDTCF-------DLSNMNEVKVPTVVLHF- 485

Query: 432 NNVSVVLPQGNNFYAMAAPINSTVVKCLLFQ-TMDGVGGDNDDSDGPAGIFGSFQQQNLE 491
               V LP  N       P+++    C  F  TM G+            I G+ QQQ   
Sbjct: 486 RGADVSLPATN----YLIPVDTNGKFCFAFAGTMGGL-----------SIIGNIQQQGFR 485

Query: 492 VVYDLEKERLGFQPMDCA 499
           VVYDL   R+GF P  CA
Sbjct: 546 VVYDLASSRVGFAPGGCA 485

BLAST of CaUC01G009550 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 2.1e-30
Identity = 145/513 (28.27%), Postives = 212/513 (41.33%), Query Frame = 0

Query: 8   IATKFLSFFLLLVYVSRKTLA---ANPKTNFPTDSLVLGLVHSRTSLLIP---------K 67
           +A+ F S  L L  +S   L+   A PK  F  D     L+H R S   P         +
Sbjct: 1   MASLFSSVLLSLCLLSSLFLSNANAKPKLGFTAD-----LIH-RDSPKSPFYNPMETSSQ 60

Query: 68  KGYNSISRKRLKTMEMGSDDNVIEPLREIRDG---YLMSLTLGTPPQVIQVYMDTGSDLT 127
           +  N+I R   +       DN  +P  ++      YLM++++GTPP  I    DTGSDL 
Sbjct: 61  RLRNAIHRSVNRVFHFTEKDNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLL 120

Query: 128 WVPCGNLSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPC 187
           W  C      C DC    + +  PK        SST    +C SS C  + +        
Sbjct: 121 WTQCA----PCDDCYTQVDPLFDPKT-------SSTYKDVSCSSSQCTALENQ------- 180

Query: 188 TIAGCSLATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRF 247
             A CS        C     S++ +YG +    G +  D L + G++   P   K I   
Sbjct: 181 --ASCSTN---DNTC-----SYSLSYGDNSYTKGNIAVDTLTL-GSSDTRPMQLKNI--- 240

Query: 248 CFGC----VGASYREPIGIAGFGRGLLSLPFQLGFSHKG-FSHCFLPFKFSNNPNFSSPL 307
             GC     G   ++  GI G G G +SL  QLG S  G FS+C +P   ++  + +S +
Sbjct: 241 IIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSIDGKFSYCLVP--LTSKKDQTSKI 300

Query: 308 ILGNLAISSKDDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKG 367
             G  AI S    +  TPL+       +YY+ L+SI++G+    +    S          
Sbjct: 301 NFGTNAIVSGSGVVS-TPLIAKASQETFYYLTLKSISVGSKQIQYSGSDS-------ESS 360

Query: 368 NGGMLIDSGTTYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSF 427
            G ++IDSGTT T LP   YS+L   + S I     K+ +  +G  LCY         S 
Sbjct: 361 EGNIIIDSGTTLTLLPTEFYSELEDAVASSI--DAEKKQDPQSGLSLCY---------SA 420

Query: 428 IDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGP 487
             D ++P IT HF +   V L   N F  +     S  + C  F+               
Sbjct: 421 TGDLKVPVITMHF-DGADVKLDSSNAFVQV-----SEDLVCFAFR-----------GSPS 437

Query: 488 AGIFGSFQQQNLEVVYDLEKERLGFQPMDCASV 501
             I+G+  Q N  V YD   + + F+P DCA +
Sbjct: 481 FSIYGNVAQMNFLVGYDTVSKTVSFKPTDCAKM 437

BLAST of CaUC01G009550 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 4.7e-30
Identity = 128/442 (28.96%), Postives = 178/442 (40.27%), Query Frame = 0

Query: 64  KRLKTME-MGSDDNVIEPLREIRDG-YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSF 123
           +RL+ +E M +  + +E      DG YLM+L++GTP Q     MDTGSDL W        
Sbjct: 69  RRLQRLEAMLNGPSGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWT------- 128

Query: 124 DCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLAT 183
            CQ C +  N         F P  SS+     C S  C  + S                 
Sbjct: 129 QCQPCTQCFNQ----STPIFNPQGSSSFSTLPCSSQLCQALSSP---------------- 188

Query: 184 LVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGC----V 243
                C      + Y YG      G++  + L            +  IP   FGC     
Sbjct: 189 ----TCSNNFCQYTYGYGDGSETQGSMGTETLTF---------GSVSIPNITFGCGENNQ 248

Query: 244 GASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK 303
           G       G+ G GRG LSLP QL  +   FS+C  P   S   N    L+LG+LA +S 
Sbjct: 249 GFGQGNGAGLVGMGRGPLSLPSQLDVTK--FSYCMTPIGSSTPSN----LLLGSLA-NSV 308

Query: 304 DDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGT 363
                 T L++S   P +YYI L  +++G+         +F L      G GG++IDSGT
Sbjct: 309 TAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPID-PSAFALN--SNNGTGGIIIDSGT 368

Query: 364 TYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSIT 423
           T T+     Y  +     S I  P       ++GFDLC++ P   +N       Q+P+  
Sbjct: 369 TLTYFVNNAYQSVRQEFISQINLPVVN--GSSSGFDLCFQTPSDPSNL------QIPTFV 428

Query: 424 FHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQ 483
            HF +   + LP  N F    +P N  +  CL              S     IFG+ QQQ
Sbjct: 429 MHF-DGGDLELPSENYF---ISPSNGLI--CLAM----------GSSSQGMSIFGNIQQQ 436

Query: 484 NLEVVYDLEKERLGFQPMDCAS 500
           N+ VVYD     + F    C +
Sbjct: 489 NMLVVYDTGNSVVSFASAQCGA 436

BLAST of CaUC01G009550 vs. ExPASy TrEMBL
Match: A0A5A7TNC9 (Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold129G00970 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.1e-273
Identity = 462/517 (89.36%), Postives = 488/517 (94.39%), Query Frame = 0

Query: 1   MPSI-STSIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYN 60
           MPSI STSIATKFLS FLLLV+ S++TLA NPKTNFP DSLVLGLVHSRTSLL PKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  SISRKRLKTM-EMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
            IS+KR+K M +M  DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+ GPKLAAFLPTHSSTSIR+TCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHG----NNINSPNSTKQIPRFCF 240
           LATLVKG CPRPCPSFAYTYGASGVV G+LTRDVLFMHG    NN N+ N+ KQ+PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGA+YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKD++LQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLP 420
           SGTTYTHLPEPLYSQLISNLESVI+YPRAKQVELNTGFDLCYKVPC+NNN SF+DDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDND-DSDGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLL+Q+MDGVG DND D +GPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLEVVYDLEKERLGFQPMDCASVAATQGLHKNV 511
           FQQQNL+VVYDLEKERLGFQ MDC SVAA QGLHKNV
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of CaUC01G009550 vs. ExPASy TrEMBL
Match: A0A1S3CAK9 (aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.1e-273
Identity = 462/517 (89.36%), Postives = 488/517 (94.39%), Query Frame = 0

Query: 1   MPSI-STSIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYN 60
           MPSI STSIATKFLS FLLLV+ S++TLA NPKTNFP DSLVLGLVHSRTSLL PKKGYN
Sbjct: 1   MPSISSTSIATKFLSLFLLLVHASKQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  SISRKRLKTM-EMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
            IS+KR+K M +M  DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQMDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+ GPKLAAFLPTHSSTSIR+TCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPKLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHG----NNINSPNSTKQIPRFCF 240
           LATLVKG CPRPCPSFAYTYGASGVV G+LTRDVLFMHG    NN N+ N+ KQ+PRFCF
Sbjct: 181 LATLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFMHGNYHNNNNNNSNNNKQVPRFCF 240

Query: 241 GCVGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAI 300
           GCVGA+YREPIGIAGFGRGLLSLPFQLGFS KGFSHCFLPFKFSNNPNFSSPLILG+LAI
Sbjct: 241 GCVGATYREPIGIAGFGRGLLSLPFQLGFSQKGFSHCFLPFKFSNNPNFSSPLILGHLAI 300

Query: 301 SSKDDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360
           SSKD++LQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID
Sbjct: 301 SSKDENLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLID 360

Query: 361 SGTTYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLP 420
           SGTTYTHLPEPLYSQLISNLESVI+YPRAKQVELNTGFDLCYKVPC+NNN SF+DDSQLP
Sbjct: 361 SGTTYTHLPEPLYSQLISNLESVISYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDSQLP 420

Query: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDND-DSDGPAGIFGS 480
           SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLL+Q+MDGVG DND D +GPAGIFGS
Sbjct: 421 SITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGS 480

Query: 481 FQQQNLEVVYDLEKERLGFQPMDCASVAATQGLHKNV 511
           FQQQNL+VVYDLEKERLGFQ MDC SVAA QGLHKNV
Sbjct: 481 FQQQNLQVVYDLEKERLGFQAMDCVSVAANQGLHKNV 517

BLAST of CaUC01G009550 vs. ExPASy TrEMBL
Match: A0A0A0LYP0 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G704590 PE=3 SV=1)

HSP 1 Score: 945.7 bits (2443), Expect = 8.2e-272
Identity = 456/513 (88.89%), Postives = 484/513 (94.35%), Query Frame = 0

Query: 1   MPSIST-SIATKFLSFFLLLVYVSRKTLAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYN 60
           MPSIS+ S ATKFLS FLLLV+VS +TLA NPKTNFP DSLVLGLVHSRTSLL PKKGYN
Sbjct: 1   MPSISSISTATKFLSLFLLLVHVSTQTLATNPKTNFPKDSLVLGLVHSRTSLLTPKKGYN 60

Query: 61  SISRKRLKTMEM-GSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGN 120
            IS+KR+K M+    DDNVIEPLREIRDGYLMSL++GTPPQV+QVYMDTGSDLTWVPCGN
Sbjct: 61  FISKKRMKAMDQTDGDDNVIEPLREIRDGYLMSLSIGTPPQVVQVYMDTGSDLTWVPCGN 120

Query: 121 LSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCS 180
           LSFDCQDCEEYQNN+ GP+LAAFLPTHSSTSIR+TCGSSFCMDIHSSDNPFDPCTIAGCS
Sbjct: 121 LSFDCQDCEEYQNNISGPRLAAFLPTHSSTSIRDTCGSSFCMDIHSSDNPFDPCTIAGCS 180

Query: 181 LATLVKGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVG 240
           LA+LVKG CPRPCPSFAYTYGASGVV G+LTRDVLF HGN  N+ N+ KQIPRFCFGCVG
Sbjct: 181 LASLVKGTCPRPCPSFAYTYGASGVVTGSLTRDVLFTHGNYNNNNNNNKQIPRFCFGCVG 240

Query: 241 ASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300
           A+YREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD
Sbjct: 241 ATYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKD 300

Query: 301 DHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360
           ++LQFTPLLKSP+YPNYYYIGLESITIGNG+NNFRFGVSFKLREIDTKGNGGMLIDSGTT
Sbjct: 301 ENLQFTPLLKSPMYPNYYYIGLESITIGNGDNNFRFGVSFKLREIDTKGNGGMLIDSGTT 360

Query: 361 YTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITF 420
           YTHLPEPLYSQLISNLE VI YPRAKQVELNTGFDLCYKVPC+NNN SF+DD+QLPSITF
Sbjct: 361 YTHLPEPLYSQLISNLELVIGYPRAKQVELNTGFDLCYKVPCKNNNSSFVDDAQLPSITF 420

Query: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDND-DSDGPAGIFGSFQQQ 480
           HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLL+Q+MDGVG DND D +GPAGIFGSFQQQ
Sbjct: 421 HFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLYQSMDGVGDDNDSDDNGPAGIFGSFQQQ 480

Query: 481 NLEVVYDLEKERLGFQPMDCASVAATQGLHKNV 511
           N+EVVYDLEKERLGFQPMDC SVAA QGLHKNV
Sbjct: 481 NIEVVYDLEKERLGFQPMDCVSVAAKQGLHKNV 513

BLAST of CaUC01G009550 vs. ExPASy TrEMBL
Match: A0A6J1KLG7 (probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111495254 PE=3 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.5e-244
Identity = 420/497 (84.51%), Postives = 453/497 (91.15%), Query Frame = 0

Query: 15  FFLLLVYVSRKTLA---ANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNSISRKRLKTMEM 74
           F L+LV VS + +    ANPKT F  DSLVLGLVHSRTSLL PK+GYNS+SRKR+K MEM
Sbjct: 10  FVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRGYNSLSRKRIKPMEM 69

Query: 75  GSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQN 134
           G DD+VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDC+EYQN
Sbjct: 70  G-DDDVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCDEYQN 129

Query: 135 NVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNCPRPC 194
           NVLGPKLAAFLPTHSSTSIRETCGSSFC+DIHSSDNPFDPCTIAGCSLATLVKG CPRPC
Sbjct: 130 NVLGPKLAAFLPTHSSTSIRETCGSSFCIDIHSSDNPFDPCTIAGCSLATLVKGACPRPC 189

Query: 195 PSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPIGIAGFG 254
           PSF+YTYGASG+VIGTLT+D +F+HG   NSPNS+++IP+FCFGCVGA+YREPIGIAGFG
Sbjct: 190 PSFSYTYGASGLVIGTLTKDAIFIHG---NSPNSSRKIPKFCFGCVGATYREPIGIAGFG 249

Query: 255 RGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDHLQFTPLLKSPI 314
           RGLLSLP QLGFSHKGFSHCFLPFKFSNNP FSSPLILGNLAISSK +HL+FTPLLKSP 
Sbjct: 250 RGLLSLPSQLGFSHKGFSHCFLPFKFSNNPKFSSPLILGNLAISSK-EHLKFTPLLKSPF 309

Query: 315 YPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLI 374
           YPNYYYIGLESITIGNG N  RFGVS +LREIDTKGNGG+LIDSGTTYTHLPEPLYSQLI
Sbjct: 310 YPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTYTHLPEPLYSQLI 369

Query: 375 SNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVSVVLPQG 434
           S LES+I+YPRAK+ ELNTGFDLCYKVP +NN F F D+ +LPSITFHFLNNVSVVLPQG
Sbjct: 370 SILESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSITFHFLNNVSVVLPQG 429

Query: 435 NNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDLEKERLG 494
           N+FYAMAAP NSTVVKCLLFQ+MDG      D DGPAGIFGSFQQQNLEVVYDLEKERLG
Sbjct: 430 NSFYAMAAPSNSTVVKCLLFQSMDG------DGDGPAGIFGSFQQQNLEVVYDLEKERLG 489

Query: 495 FQPMDCASVAATQGLHK 509
           F+ MDCASVA +QGLHK
Sbjct: 490 FEAMDCASVAVSQGLHK 494

BLAST of CaUC01G009550 vs. ExPASy TrEMBL
Match: A0A6J1EHM1 (probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC111434252 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.3e-244
Identity = 419/503 (83.30%), Postives = 454/503 (90.26%), Query Frame = 0

Query: 9   ATKFLSFFLLLVYVSRKTLA---ANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNSISRKR 68
           A  +    L+LV VS + +    ANPKT F  DSLVLGLVHSRTSLL PK+GYNS+  KR
Sbjct: 6   ARSYFVLVLVLVLVSGEAMGQTLANPKTKFLKDSLVLGLVHSRTSLLTPKRGYNSLLTKR 65

Query: 69  LKTMEMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQD 128
           +K MEMG+DD VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQD
Sbjct: 66  IKPMEMGNDD-VIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQD 125

Query: 129 CEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKG 188
           C+EYQNNVLGPKLAAFLPTHSSTSIR+TCGSSFC+DIHSSDNPFDPCTIAGCSLATLVKG
Sbjct: 126 CDEYQNNVLGPKLAAFLPTHSSTSIRDTCGSSFCIDIHSSDNPFDPCTIAGCSLATLVKG 185

Query: 189 NCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPI 248
            CPRPCPSF+YTYGASG+VIGTLT+DV+F+HG   NSPNS+++IP+FCFGCVGA+YREPI
Sbjct: 186 TCPRPCPSFSYTYGASGLVIGTLTKDVIFIHG---NSPNSSRKIPKFCFGCVGATYREPI 245

Query: 249 GIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSKDDHLQFTP 308
           GIAGFGRGLLSLP QLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK +HL+FTP
Sbjct: 246 GIAGFGRGLLSLPSQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAISSK-EHLKFTP 305

Query: 309 LLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEP 368
            LKSP YPNYYYIGLESITIGNG N  RFGVS +LREIDTKGNGG+LIDSGTTYTHLPEP
Sbjct: 306 FLKSPFYPNYYYIGLESITIGNGENYSRFGVSLQLREIDTKGNGGILIDSGTTYTHLPEP 365

Query: 369 LYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVS 428
           LYSQLISNLES+I+YPRAK+ ELNTGFDLCYKVP +NN F F D+ +LPSITFHFLNNVS
Sbjct: 366 LYSQLISNLESLISYPRAKEHELNTGFDLCYKVPYKNNTF-FSDEFELPSITFHFLNNVS 425

Query: 429 VVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDL 488
           VVLPQGN+FYAMAAP NSTVVKCLLFQ+MDG      D DGPAGIFGSFQQQNLEVVYDL
Sbjct: 426 VVLPQGNSFYAMAAPSNSTVVKCLLFQSMDG------DGDGPAGIFGSFQQQNLEVVYDL 485

Query: 489 EKERLGFQPMDCASVAATQGLHK 509
           EKERLGF+ MDCASVA +QGLHK
Sbjct: 486 EKERLGFEAMDCASVAVSQGLHK 496

BLAST of CaUC01G009550 vs. TAIR 10
Match: AT5G45120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 592.8 bits (1527), Expect = 2.6e-169
Identity = 303/508 (59.65%), Postives = 371/508 (73.03%), Query Frame = 0

Query: 10  TKFLSFFL---LLVYVSRKTLAANPKTNFPTDS--LVLGLVHSRTSLLIPKKGYNSISRK 69
           T  L  FL   LL+  + KT A   K    + S  LVL L  S  SL  PK    S +++
Sbjct: 5   THVLFLFLLITLLLNTTNKTQARQHKNPSSSSSSFLVLTLTKSSVSLPTPK----SQTQE 64

Query: 70  RLKTMEMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQ 129
           R+K   + S D V+EPLRE+RDGYL++L +GTPPQ +QVY+DTGSDLTWVPCGNLSFDC 
Sbjct: 65  RIK-KPLSSVDVVMEPLREVRDGYLITLNIGTPPQAVQVYLDTGSDLTWVPCGNLSFDCI 124

Query: 130 DCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVK 189
           +C + +NN L    + F P HSSTS R++C SSFC++IHSSDNPFDPC +AGCS++ L+K
Sbjct: 125 ECYDLKNNDL-KSPSVFSPLHSSTSFRDSCASSFCVEIHSSDNPFDPCAVAGCSVSMLLK 184

Query: 190 GNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREP 249
             C RPCPSFAYTYG  G++ G LTRD+L            T+ +PRF FGCV ++YREP
Sbjct: 185 STCVRPCPSFAYTYGEGGLISGILTRDIL---------KARTRDVPRFSFGCVTSTYREP 244

Query: 250 IGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFKFSNNPNFSSPLILGNLAIS-SKDDHLQF 309
           IGIAGFGRGLLSLP QLGF  KGFSHCFLPFKF NNPN SSPLILG  A+S +  D LQF
Sbjct: 245 IGIAGFGRGLLSLPSQLGFLEKGFSHCFLPFKFVNNPNISSPLILGASALSINLTDSLQF 304

Query: 310 TPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLP 369
           TP+L +P+YPN YYIGLESITI  G N     V   LR+ D++GNGGML+DSGTTYTHLP
Sbjct: 305 TPMLNTPMYPNSYYIGLESITI--GTNITPTQVPLTLRQFDSQGNGGMLVDSGTTYTHLP 364

Query: 370 EPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQL---PSITFHF 429
           EP YSQL++ L+S I YPRA + E  TGFDLCYKVPC NNN + +++  +   PSITFHF
Sbjct: 365 EPFYSQLLTTLQSTITYPRATETESRTGFDLCYKVPCPNNNLTSLENDVMMIFPSITFHF 424

Query: 430 LNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLE 489
           LNN +++LPQGN+FYAM+AP + +VV+CLLFQ M+      D   GPAG+FGSFQQQN++
Sbjct: 425 LNNATLLLPQGNSFYAMSAPSDGSVVQCLLFQNME------DGDYGPAGVFGSFQQQNVK 484

Query: 490 VVYDLEKERLGFQPMDCASVAATQGLHK 509
           VVYDLEKER+GFQ MDC   AA+ GL++
Sbjct: 485 VVYDLEKERIGFQAMDCVLEAASHGLNQ 489

BLAST of CaUC01G009550 vs. TAIR 10
Match: AT4G16563.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 220.7 bits (561), Expect = 2.7e-57
Identity = 154/447 (34.45%), Postives = 217/447 (48.55%), Query Frame = 0

Query: 88  YLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVLGPKLAAFLPTHSS 147
           YL+SL++G+    + +Y+DTGSDL W PC    F C  CE   +  L P   + L   SS
Sbjct: 83  YLISLSVGSSSSAVSLYLDTGSDLVWFPC--RPFTCILCE---SKPLPPSPPSSL---SS 142

Query: 148 TSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNC---PRPCPSFAYTYGASGVV 207
           ++   +C S  C   HSS    D C I+ C L  +  G+C     PCP F Y YG  G +
Sbjct: 143 SATTVSCSSPSCSAAHSSLPSSDLCAISNCPLDFIETGDCNTSSYPCPPFYYAYG-DGSL 202

Query: 208 IGTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGF- 267
           +  L  D L +   ++++         F FGC   +  EPIG+AGFGRG LSLP QL   
Sbjct: 203 VAKLYSDSLSLPSVSVSN---------FTFGCAHTTLAEPIGVAGFGRGRLSLPAQLAVH 262

Query: 268 -SHKG--FSHCFLPFKF-SNNPNFSSPLILGNLA------ISSKDDH------------L 327
             H G  FS+C +   F S+     SPLILG         + + DDH             
Sbjct: 263 SPHLGNSFSYCLVSHSFDSDRVRRPSPLILGRFVDKKEKRVGTTDDHDDGDDEKKKKNEF 322

Query: 328 QFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTH 387
            FT +L++P +P +Y + L+ I+IG  N          LR ID  G GG+++DSGTT+T 
Sbjct: 323 VFTEMLENPKHPYFYSVSLQGISIGKRN----IPAPAMLRRIDKNGGGGVVVDSGTTFTM 382

Query: 388 LPEPLYSQLISNLESVI--AYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFH 447
           LP   Y+ ++   +S +   + RA +VE ++G   CY +             ++P++  H
Sbjct: 383 LPAKFYNSVVEEFDSRVGRVHERADRVEPSSGMSPCYYLN---------QTVKVPALVLH 442

Query: 448 FL-NNVSVVLPQGNNFYAMA----APINSTVVKCLLFQTMDGVGGDNDDSDGPAG-IFGS 501
           F  N  SV LP+ N FY              + CL+       GGD  +  G  G I G+
Sbjct: 443 FAGNRSSVTLPRRNYFYEFMDGGDGKEEKRKIGCLMLMN----GGDESELRGGTGAILGN 494

BLAST of CaUC01G009550 vs. TAIR 10
Match: AT3G52500.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 183.3 bits (464), Expect = 4.8e-46
Identity = 139/422 (32.94%), Postives = 202/422 (47.87%), Query Frame = 0

Query: 87  GYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVLGPKL-AAFLPTH 146
           GY +SL+ GTP Q I    DTGS L W+PC +  + C  C+    + L P L   F+P +
Sbjct: 89  GYSVSLSFGTPSQTIPFVFDTGSSLVWLPCTS-RYLCSGCD---FSGLDPTLIPRFIPKN 148

Query: 147 SSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLVKGNCPRPCPSFAYTYGASGVVI 206
           SS+S    C S  C  ++    P   C   GC   T    NC   CP +   YG  G   
Sbjct: 149 SSSSKIIGCQSPKCQFLY---GPNVQC--RGCDPNT---RNCTVGCPPYILQYGL-GSTA 208

Query: 207 GTLTRDVLFMHGNNINSPNSTKQIPRFCFGCVGASYREPIGIAGFGRGLLSLPFQLGFSH 266
           G L  +        ++ P+ T  +P F  GC   S R+P GIAGFGRG +SLP Q+    
Sbjct: 209 GVLITE-------KLDFPDLT--VPDFVVGCSIISTRQPAGIAGFGRGPVSLPSQMNL-- 268

Query: 267 KGFSHCFLPFKFSNNPNFSSPLILGNLA---ISSKDDHLQFTPLLKSPIYPN-----YYY 326
           K FSHC +  +F ++ N ++ L L   +     SK   L +TP  K+P   N     YYY
Sbjct: 269 KRFSHCLVSRRF-DDTNVTTDLDLDTGSGHNSGSKTPGLTYTPFRKNPNVSNKAFLEYYY 328

Query: 327 IGLESITIGNGNNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESV 386
           + L  I +G  +      + +K     T G+GG ++DSG+T+T +  P++  +     S 
Sbjct: 329 LNLRRIYVGRKH----VKIPYKYLAPGTNGDGGSIVDSGSTFTFMERPVFELVAEEFASQ 388

Query: 387 IA-YPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVSVVLPQGNNFYA 446
           ++ Y R K +E  TG   C+       N S   D  +P + F F     + LP  +N++ 
Sbjct: 389 MSNYTREKDLEKETGLGPCF-------NISGKGDVTVPELIFEFKGGAKLELPL-SNYFT 448

Query: 447 MAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMD 499
                ++  +  +  +T++  GG      GPA I GSFQQQN  V YDLE +R GF    
Sbjct: 449 FVGNTDTVCLTVVSDKTVNPSGG-----TGPAIILGSFQQQNYLVEYDLENDRFGFAKKK 468

BLAST of CaUC01G009550 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 154.5 bits (389), Expect = 2.4e-37
Identity = 142/506 (28.06%), Postives = 202/506 (39.92%), Query Frame = 0

Query: 12  FLSFFLL----LVYVSRKT----LAANPKTNFPTDSLVLGLVHSRTSLLIPKKGYNSISR 71
           FLS FLL    +  VS       L    K+ FP+ +  L L   R   L       S+ R
Sbjct: 11  FLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFL-------SLRR 70

Query: 72  KRLKTMEMGSDDNVIEPLREIRDGYLMSLTLGTPPQVIQVYMDTGSDLTWVPCGNLSFDC 131
           K +  ++      V+         Y + L +G PPQ + +  DTGSDL WV C      C
Sbjct: 71  KPIPFVK----SPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCS----AC 130

Query: 132 QDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDIHSSDNPFDPCTIAGCSLATLV 191
           ++C  +           F P HSST     C    C  +   D        A     T +
Sbjct: 131 RNCSHHS------PATVFFPRHSSTFSPAHCYDPVCRLVPKPDR-------APICNHTRI 190

Query: 192 KGNCPRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNINSPNSTKQIPRFCFGC------- 251
              C      + Y Y    +  G   R+   +      S     ++    FGC       
Sbjct: 191 HSTC-----HYEYGYADGSLTSGLFARETTSLK----TSSGKEARLKSVAFGCGFRISGQ 250

Query: 252 --VGASYREPIGIAGFGRGLLSLPFQLG--FSHKGFSHCFLPFKFSNNPNFSSPLILGNL 311
              G S+    G+ G GRG +S   QLG  F +K FS+C + +  S  P  +S LI+GN 
Sbjct: 251 SVSGTSFNGANGVMGLGRGPISFASQLGRRFGNK-FSYCLMDYTLSPPP--TSYLIIGN- 310

Query: 312 AISSKDDHLQFTPLLKSPIYPNYYYIGLESITIGNGNNNFRFGVSFKLREIDTKGNGGML 371
                   L FTPLL +P+ P +YY+ L+S+ +    N  +  +   + EID  GNGG +
Sbjct: 311 -GGDGISKLFFTPLLTNPLSPTFYYVKLKSVFV----NGAKLRIDPSIWEIDDSGNGGTV 370

Query: 372 IDSGTTYTHLPEPLYSQLISNLESVIAYPRAKQVELNTGFDLCYKVPCRNNNFSFIDDSQ 431
           +DSGTT   L EP Y  +I+ +   +  P A    L  GFDLC  V           +  
Sbjct: 371 VDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD--ALTPGFDLCVNVSGVTK-----PEKI 430

Query: 432 LPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKCLLFQTMDGVGGDNDDSDGPAGIFG 491
           LP + F F      V P  N F           ++CL  Q++D   G          + G
Sbjct: 431 LPRLKFEFSGGAVFVPPPRNYFIE-----TEEQIQCLAIQSVDPKVG--------FSVIG 450

Query: 492 SFQQQNLEVVYDLEKERLGFQPMDCA 499
           +  QQ     +D ++ RLGF    CA
Sbjct: 491 NLMQQGFLFEFDRDRSRLGFSRRGCA 450

BLAST of CaUC01G009550 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 149.8 bits (377), Expect = 5.8e-36
Identity = 156/532 (29.32%), Postives = 222/532 (41.73%), Query Frame = 0

Query: 5   STSIATKFLSFFLLL------VYVSRKTLAAN--PKTNFPTDSLVLGLVH--SRTSLLIP 64
           S+S ++    FFL+L      V  SR++L     PK N P     L L H  S  +L   
Sbjct: 3   SSSSSSLLFPFFLILFSCLISVSSSRRSLIDRTLPK-NLPRSGFRLSLRHVDSGKNLTKI 62

Query: 65  KKGYNSISRKRLKTMEMGS----------DD--NVIEPLREIRDGYLMSLTLGTPPQVIQ 124
           +K    I+R   +   +G+          DD  N+  P       +LM L++G P     
Sbjct: 63  QKIQRGINRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYS 122

Query: 125 VYMDTGSDLTWVPCGNLSFDCQDCEEYQNNVLGPKLAAFLPTHSSTSIRETCGSSFCMDI 184
             +DTGSDL W  C      C +C +    +       F P  SS+  +  C S  C   
Sbjct: 123 AIVDTGSDLIWTQCK----PCTECFDQPTPI-------FDPEKSSSYSKVGCSSGLC--- 182

Query: 185 HSSDNPFDPCTIAGCSLATLVKGNC--PRPCPSFAYTYGASGVVIGTLTRDVLFMHGNNI 244
                              L + NC   +    + YTYG      G L  +       N 
Sbjct: 183 -----------------NALPRSNCNEDKDACEYLYTYGDYSSTRGLLATETFTFEDEN- 242

Query: 245 NSPNSTKQIPRFCFGC----VGASYREPIGIAGFGRGLLSLPFQLGFSHKGFSHCFLPFK 304
                   I    FGC     G  + +  G+ G GRG LSL  QL      FS+C    +
Sbjct: 243 -------SISGIGFGCGVENEGDGFSQGSGLVGLGRGPLSLISQL--KETKFSYCLTSIE 302

Query: 305 FSNNPNFSSPLILGNLAI-------SSKDDHLQFT-PLLKSPIYPNYYYIGLESITIGNG 364
            S     SS L +G+LA        +S D  +  T  LL++P  P++YY+ L+ IT+G  
Sbjct: 303 DS---EASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPDQPSFYYLELQGITVGAK 362

Query: 365 NNNFRFGVSFKLREIDTKGNGGMLIDSGTTYTHLPEPLYSQLISNLESVIAYPRAKQVEL 424
               R  V     E+   G GGM+IDSGTT T+L E  +  L     S ++ P       
Sbjct: 363 ----RLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFTSRMSLP--VDDSG 422

Query: 425 NTGFDLCYKVPCRNNNFSFIDDSQLPSITFHFLNNVSVVLPQGNNFYAMAAPINSTVVKC 484
           +TG DLC+K+P    N +      +P + FHF     + LP G N+  M A  +ST V C
Sbjct: 423 STGLDLCFKLPDAAKNIA------VPKMIFHF-KGADLELP-GENY--MVAD-SSTGVLC 461

Query: 485 LLFQTMDGVGGDNDDSDGPAGIFGSFQQQNLEVVYDLEKERLGFQPMDCASV 501
           L   + +G+            IFG+ QQQN  V++DLEKE + F P +C  +
Sbjct: 483 LAMGSSNGM-----------SIFGNVQQQNFNVLHDLEKETVSFVPTECGKL 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893627.12.9e-27992.77probable aspartyl protease At4g16563 [Benincasa hispida][more]
XP_008459091.12.4e-27389.36PREDICTED: aspartic proteinase nepenthesin-2 [Cucumis melo] >KAA0043307.1 aspart... [more]
XP_004145478.21.7e-27188.89probable aspartyl protease At4g16563 [Cucumis sativus] >KGN66888.1 hypothetical ... [more]
XP_023520027.11.1e-24683.27probable aspartyl protease At4g16563 [Cucurbita pepo subsp. pepo][more]
KAG6583807.13.6e-24584.37putative aspartyl protease, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q940R43.8e-5634.45Probable aspartyl protease At4g16563 OS=Arabidopsis thaliana OX=3702 GN=At4g1656... [more]
Q766C22.6e-3327.88Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q9LNJ33.4e-3328.31Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q6XBF82.1e-3028.27Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1[more]
Q766C34.7e-3028.96Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7TNC91.1e-27389.36Aspartic proteinase nepenthesin-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3CAK91.1e-27389.36aspartic proteinase nepenthesin-2 OS=Cucumis melo OX=3656 GN=LOC103498305 PE=3 S... [more]
A0A0A0LYP08.2e-27288.89Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G70459... [more]
A0A6J1KLG72.5e-24484.51probable aspartyl protease At4g16563 OS=Cucurbita maxima OX=3661 GN=LOC111495254... [more]
A0A6J1EHM14.3e-24483.30probable aspartyl protease At4g16563 OS=Cucurbita moschata OX=3662 GN=LOC1114342... [more]
Match NameE-valueIdentityDescription
AT5G45120.12.6e-16959.65Eukaryotic aspartyl protease family protein [more]
AT4G16563.12.7e-5734.45Eukaryotic aspartyl protease family protein [more]
AT3G52500.14.8e-4632.94Eukaryotic aspartyl protease family protein [more]
AT3G25700.12.4e-3728.06Eukaryotic aspartyl protease family protein [more]
AT2G03200.15.8e-3629.32Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 82..290
e-value: 8.3E-31
score: 109.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 291..504
e-value: 4.6E-46
score: 158.8
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 87..499
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 88..291
e-value: 2.1E-27
score: 96.5
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 316..493
e-value: 7.8E-27
score: 94.0
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 58..502
NoneNo IPR availablePANTHERPTHR47967:SF47CHLOROPLAST NUCLEOID DNA-BINDING PROTEIN-LIKEcoord: 58..502
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 351..362
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 88..493
score: 33.938034
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 87..497
e-value: 4.27531E-72
score: 228.301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC01G009550.1CaUC01G009550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity