CSPI07G22920 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G22920
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionCytochrome p450
LocationChr7: 19359284 .. 19363027 (+)
RNA-Seq ExpressionCSPI07G22920
SyntenyCSPI07G22920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAGTTTATGGGATGTTTTGGTAGTGACAGCCATTTTTTTTACCACTATTATTCTCTATAGGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTTCCTTTGGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCAGCGCTTATTCAGACCGTCCTGAAACTTTCATGGACAAGCGACGCCGCCTGTAAGTTATCCACAAAAACCATGTTCAAATGTCTTCAAATGCTAATATGATACGACTTGTGAATGTTTGCAGGTACGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACCATTGTCTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCTAAGGCCTTTGTACCGTCTTATCCGAAATCTTTGACAGAATTGATGGGGAAGTCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACATCTCAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTATCAATGGCAAACTGGACTGAGAACAATCCGATTTATCTTCAAGATGAAACCAAAAATGTCAGCTCTGTCTCTCTCTCTCTTCCCCTTTTGAAGCTTGAAATTTACCTATGAATTATTTTTAATTTTTCCCCCTCAACAAAAGGACAATAAGATAAAAATTTCCTATCATTACTGTATTGATACTAGACAAAAACAGTGACTTCCTTTAACTGATCTGAGTGAATCATTGATATCCAAATAACCCATTTCTCTAGCTCCTACTTGGTTTTAAAGATGATTTCAGTCAAAAGTAGGAGAACCCCACAATTTTTGCTATCATTCTGTTTCAAATCATATTGTCCAATAATTTTACCTTTGTAATCATTTTTAGCTATCGATTCTCCTGTTAAAAAAGTTACTAAGTGAAACCCCTTTTGTCTCCAGATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGTATGGAGTTTCTCAAGAAACAGTTTCAAGAGTTCATAGCTGGGCTTATGGCGTTGCCAGTGAACATACCTGGAAGCAGGCTTTACCAATCCTTGCAGGCAAGTTGGATTGTAAAAGGTTTAATATCCTCCTTCATTCGTAGTTTTTTAGATCATTCTAAAAAATGATAGAGCTTTATTTTGTTCTTGAATTTCAGGCCAAAAGGAATATGGTCAAACTAGTAAAGAAGATAATACAAGAGAGGAAAAGCTGTGGAATTTCCACTGTCCCAAAAGATATAATGGAAGTTTTGCTCAAAGATGAAAATGAAGAACTAACAGATGATCTAATAGCAGAGAACATGATTGATATGATGATTCCCGGAGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTGAAATACCTCTCTGATTGCCCTGCCGCACTTCAACAGTTGACGGTATGTCGTTTTTCTTATCAACTCTCAATCTTTTCTGTTACTGTTACTTCTGCTCTGATACCATGTCAAACTAGGAAAGTATAGACAGACAAGTTTTCATAAATTTTTTTTTCTCAATCAATATGCCAGCGTGCACAGTAGCAAATGTTGCTTTAATGAAATACAAATATGCTCATCGACTTTCTCTAGTTCTGATTTGTATCACTTGAAAAGGTGATATCCTTATTTTCCTTTCAACTTTAATAATGTCCCTATCAGGAGGTTCCCTATAAGTTAAAGGTTAGGCTATTAAAGTTACTCTGAACTATGTTCTTTGTGTAAAAATATATTCCGATTCTTTTAGAAGTTGCAATATTACCCTTAATGTTTCATAAACATTTCAAACTACGTTTGGAGTAGAAATCCTTTTAGAACATTAGACAAAAATTGGGATTTAGAATAGTGTGGCGATGACTCGAAATGAAAAATAGGATCTGAAGATCACCCCATTCATTCCAGGTCTCGAAGTTCATTTTCTACCTCAAAGGCAATTTTGAAACATTTATGAAAAAATAAGGATATTTTTTTCAAAGAATAAGGGTATTTCTTGTGATTTATCCCAAGTTAAATGATTGTAGTAACACTTTGGTGGAAAACAAAATTCATTTGTTTCTTGGCTTGTTTGAGTAGTAGCTGTTAAACCATATGGCTGTTTAAACTGACATGATCAGGAGGAAAACTTGAAGATGAAAGGACAGAAAGAGCAGCTCGGTGAGGCATTGCATTGGAGCGATTACTTATCGATGCCATTTACGCAGAGTGTAATACATTGATTAAACTTTTGATAAAGTAGAATGAAACAGTAAATTATTGGGAGTGATTATATTGAATGTTATGGTTTGATTTGAATAGGTGATCACTGAAACTCTAAGGATGGGAAACATTATAATTGGAGTGATGCGCAAGGCCATGAAAGACATGAAGATAAAAGGATACCTAATACCTAAGGGTTGGTCAGTTCTTACATACTTTAGATCTGTTCATCTTGATGACAATCACTACGACTGCCCTTACAAGTTCAATCCATGGAGGTGGCAAGTAAGAAGAAAAAAATTTGTGCCTATCTCTATTCTCTCTCAGTGTTTTTGTACGTATGACTTAAAAAGTTGTGTTCGGATGAGATTTGACCAACCTTTTACAAATAAGCTTCTCTAACCTTTTGCTAATGCCACCAATCCTCTCACCCTTAGCTCTTGCTCACGTTTTCTCTCTCACTTTGACTCATGATACCCCTCTTACATGCTCACTTTCTTGGAAAAAAATAGTTTGAAAAGAGTTGGAAAAAGGTTAGAAAACTTTATTTTGAAAAATGTGATCAAATCCCATTTAGGTACAAGTTTTTAAGTGAATCCATCAAAATATTCCCTCTTTCTTTATATCTTAAACTTTTTATGTTCTCTCTCCCTAATGGTTTAGTGGTTGATCAGGACAGAGAGATGAGTAGTTGCAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTAGCCAGGCTTGAAGCTTCCATCTTCCTTCACCACTTTGTCACTCACTTCAGGTACCTCACGTCCAATATCACTTGTACAAATAGGTATCAAGTTTCTTCTAACTTGATATCATATGTTTTCTTCATAAGTAGAATACAACATTAATACTAAAGGTCTTAAATAAAACCAACCCAAACCAAAATTTGTGTTTTACTGAAGTGCCAGTTTGTTTAAACTTTGAAGGAAATCTGATTTCTCAGTTTGGTTTGGTTTGGTTTTAGATTTGAAAACTGAAAACTGAACAAAACCATTTATATTATTGAAAACCAAGATAAAAAAGATTTCATATGGTATATATTCCACAGATGGATTGCTGAAGAAGATAAAATCATAAACTTCCCCACAGTGAGGATGAAAAAGAGGATGCCAGTTTGGGTGAAGAGAAGAAGGGACCCATGACCAGACAATAATGGCAAGAGAAGATTCCCTTTTTGCCAAAAGGAAAAGAAAAGAAAAAAATGCACAAAACTTCAATTAACTTCACCATTAGTTTACACAACAACCAAACTTACCTTTGGAACAAGTAGAGAAAAAAGAAAAGAAAAGAAGTCTCACCTCTAACAAAGGGACTATGGGTTAAAAGATGACAGTTCAGTTGACATATGACACTTCTATCCTCTCCTATGCCCACCTCCCTATTGCAACCCATAATAATCCATTTGTCACTTATTGTATATCATATCTATGTGTGTATGTAT

mRNA sequence

ATGGACAGTTTATGGGATGTTTTGGTAGTGACAGCCATTTTTTTTACCACTATTATTCTCTATAGGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTTCCTTTGGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCAGCGCTTATTCAGACCGTCCTGAAACTTTCATGGACAAGCGACGCCGCCTGTACGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACCATTGTCTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCTAAGGCCTTTGTACCGTCTTATCCGAAATCTTTGACAGAATTGATGGGGAAGTCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACATCTCAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTATCAATGGCAAACTGGACTGAGAACAATCCGATTTATCTTCAAGATGAAACCAAAAATATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGTATGGAGTTTCTCAAGAAACAGTTTCAAGAGTTCATAGCTGGGCTTATGGCGTTGCCAGTGAACATACCTGGAAGCAGGCTTTACCAATCCTTGCAGGCCAAAAGGAATATGGTCAAACTAGTAAAGAAGATAATACAAGAGAGGAAAAGCTGTGGAATTTCCACTGTCCCAAAAGATATAATGGAAGTTTTGCTCAAAGATGAAAATGAAGAACTAACAGATGATCTAATAGCAGAGAACATGATTGATATGATGATTCCCGGAGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTGAAATACCTCTCTGATTGCCCTGCCGCACTTCAACAGTTGACGGAGGAAAACTTGAAGATGAAAGGACAGAAAGAGCAGCTCGGTGAGGCATTGCATTGGAGCGATTACTTATCGATGCCATTTACGCAGAGTGTGATCACTGAAACTCTAAGGATGGGAAACATTATAATTGGAGTGATGCGCAAGGCCATGAAAGACATGAAGATAAAAGGATACCTAATACCTAAGGGTTGGTCAGTTCTTACATACTTTAGATCTGTTCATCTTGATGACAATCACTACGACTGCCCTTACAAGTTCAATCCATGGAGGTGGCAAGACAGAGAGATGAGTAGTTGCAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTAGCCAGGCTTGAAGCTTCCATCTTCCTTCACCACTTTGTCACTCACTTCAGATGGATTGCTGAAGAAGATAAAATCATAAACTTCCCCACAGTGAGGATGAAAAAGAGGATGCCAGTTTGGGTGAAGAGAAGAAGGGACCCATGACCAGACAATAATGGCAAGAGAAGATTCCCTTTTTGCCAAAAGGAAAAGAAAAGAAAAAAATGCACAAAACTTCAATTAACTTCACCATTAGTTTACACAACAACCAAACTTACCTTTGGAACAAGTAGAGAAAAAAGAAAAGAAAAGAAGTCTCACCTCTAACAAAGGGACTATGGGTTAAAAGATGACAGTTCAGTTGACATATGACACTTCTATCCTCTCCTATGCCCACCTCCCTATTGCAACCCATAATAATCCATTTGTCACTTATTGTATATCATATCTATGTGTGTATGTAT

Coding sequence (CDS)

ATGGACAGTTTATGGGATGTTTTGGTAGTGACAGCCATTTTTTTTACCACTATTATTCTCTATAGGAACTGCTTTAGACTTCTCAGATCAAAATTCTGCAACCAACTTCCTTTGGGCTCTCTTGGCTGGCCTTTCATTGGTGAGACTATTGAGTTCATCTCCAGCGCTTATTCAGACCGTCCTGAAACTTTCATGGACAAGCGACGCCGCCTGTACGGGAAGGTGTTCAAGTCACATATTTTTGGAAGTCCTACCATTGTCTCTACTGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCTAAGGCCTTTGTACCGTCTTATCCGAAATCTTTGACAGAATTGATGGGGAAGTCTTCTATTTTGCTGATAAATGGGAGCTTGCAGAAGAGAATTCATGGTCTTGTGGGAGCTTTCTTCAAGTCTTCACATCTCAAAGCTCAAATAACCAAGGATATGGAGTGTTATGTCAAACTATCAATGGCAAACTGGACTGAGAACAATCCGATTTATCTTCAAGATGAAACCAAAAATATTTCCTTTCAAGTATTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGTATGGAGTTTCTCAAGAAACAGTTTCAAGAGTTCATAGCTGGGCTTATGGCGTTGCCAGTGAACATACCTGGAAGCAGGCTTTACCAATCCTTGCAGGCCAAAAGGAATATGGTCAAACTAGTAAAGAAGATAATACAAGAGAGGAAAAGCTGTGGAATTTCCACTGTCCCAAAAGATATAATGGAAGTTTTGCTCAAAGATGAAAATGAAGAACTAACAGATGATCTAATAGCAGAGAACATGATTGATATGATGATTCCCGGAGAAGATTCAGTGCCAATCTTAATGACTCTTGCAGTGAAATACCTCTCTGATTGCCCTGCCGCACTTCAACAGTTGACGGAGGAAAACTTGAAGATGAAAGGACAGAAAGAGCAGCTCGGTGAGGCATTGCATTGGAGCGATTACTTATCGATGCCATTTACGCAGAGTGTGATCACTGAAACTCTAAGGATGGGAAACATTATAATTGGAGTGATGCGCAAGGCCATGAAAGACATGAAGATAAAAGGATACCTAATACCTAAGGGTTGGTCAGTTCTTACATACTTTAGATCTGTTCATCTTGATGACAATCACTACGACTGCCCTTACAAGTTCAATCCATGGAGGTGGCAAGACAGAGAGATGAGTAGTTGCAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTAGCCAGGCTTGAAGCTTCCATCTTCCTTCACCACTTTGTCACTCACTTCAGATGGATTGCTGAAGAAGATAAAATCATAAACTTCCCCACAGTGAGGATGAAAAAGAGGATGCCAGTTTGGGTGAAGAGAAGAAGGGACCCATGA

Protein sequence

MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP*
Homology
BLAST of CSPI07G22920 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 2.4e-174
Identity = 302/491 (61.51%), Postives = 382/491 (77.80%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCN----------------QLPLGSLGWP 60
           MD+   +L  +  FF  I+++ N    LRS   +                + P GSLGWP
Sbjct: 1   MDTSSSLLFFSFFFFIIIVIF-NKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWP 60

Query: 61  FIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKA 120
            IGETIEF+SSAYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV++ +LQSD+ A
Sbjct: 61  VIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTA 120

Query: 121 FVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMAN 180
           FVP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKAQI +DM  ++  SM  
Sbjct: 121 FVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDL 180

Query: 181 WTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRL 240
           W+E+ P+ LQD +K ++F+VL K LIS+  G+ +E LK++F+ FI+GLM+LP+N PG++L
Sbjct: 181 WSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLPINFPGTQL 240

Query: 241 YQSLQAKRNMVKLVKKIIQ-----ERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMI 300
           ++SLQAK+NMVK V++II+      +       + KD+++VLLKD +E LT +LIA NMI
Sbjct: 241 HRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHLTHNLIANNMI 300

Query: 301 DMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFT 360
           DMMIPG DSVP+L+TLAVK+LSD PAAL  LTEEN+K+K  KE  GE L+W+DYLS+PFT
Sbjct: 301 DMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLYWNDYLSLPFT 360

Query: 361 QSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNP 420
           Q VITETLRMGN+IIGVMRKAMKD++IKGY+IPKGW  L Y RSVHLD  +Y+ PYKFNP
Sbjct: 361 QKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNP 420

Query: 421 WRWQDREMSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVR 471
           WRWQ+R+M++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT FRWIAEED IINFPTV 
Sbjct: 421 WRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEEDTIINFPTVH 480

BLAST of CSPI07G22920 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 2.0e-168
Identity = 275/441 (62.36%), Postives = 373/441 (84.58%), Query Frame = 0

Query: 35  QLPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGK-VFKSHIFGSPTIVSTDAEV 94
           +LP GS GWP +GET+EF+S AYS RPE F+DKRR+L+G  VF+SH+FGS T+V+ DAEV
Sbjct: 48  RLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEV 107

Query: 95  SKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKD 154
           S+F+LQSDA+AFVP YP+SLTELMGKSSILLING+LQ+R+HGLVGAFFKSSHLK+Q+T D
Sbjct: 108 SRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTAD 167

Query: 155 MECYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLM 214
           M   +  +++++ +++ +++Q   K++ F++LV+ LI L  G+ M+ LK+QFQEFI GLM
Sbjct: 168 MRRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLM 227

Query: 215 ALPVNIPGSRLYQSLQAKRNMVKLVKKIIQERKS-CGISTVPKDIMEVLLKDENEELTDD 274
           +LP+ +PG+RLY+SLQAK+ M +L+++II+E+++    ++ P+D ++VL+ D ++ELTD+
Sbjct: 228 SLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASPPRDAIDVLIGDGSDELTDE 287

Query: 275 LIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSD 334
           LI++NMID+MIP EDSVP+L+TLAVK+LS+CP AL QL EEN+++K +K  +GE L W+D
Sbjct: 288 LISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWTD 347

Query: 335 YLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD 394
           Y+S+ FTQ VITETLR+GNII G+MRKA++D+++KG+LIPKGW V  YFRSVHLDD  YD
Sbjct: 348 YMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLYD 407

Query: 395 CPYKFNPWRWQDREMSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEEDKI 454
            PYKFNPWRW++++MS+ +FTPFGGGQRLCPGLDLARLEASIFLHH VT FRW+AEED I
Sbjct: 408 EPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVAEEDHI 467

Query: 455 INFPTVRMKKRMPVWVKRRRD 474
           +NFPTVR+K+ MP+ V  + D
Sbjct: 468 VNFPTVRLKRGMPIRVTAKED 488

BLAST of CSPI07G22920 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 526.2 bits (1354), Expect = 3.9e-148
Identity = 249/442 (56.33%), Postives = 344/442 (77.83%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FI+  YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
            +LQ+    FVP+YPKS+TEL+G++SIL ING  QKR+H L+GAF +S HLK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMAL 215
             V L++A+W +   +++QDE K ++F++LVK+L+S +PG+ M  LK +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 216 PVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTVP-KDIMEVLLKD----ENEELT 275
           P+  PG+RLY+SL+AK  ++K+VKK+++ER+    +T P  D+++VLL+D    E +   
Sbjct: 249 PIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQP 308

Query: 276 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 335
            D ++  +++MMIPGE+++P  MTLAVK+LSD P AL +L EEN++MK +K +LGE   W
Sbjct: 309 SDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKW 368

Query: 336 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 395
           +DY+S+ FTQ+VI ETLRM NII GV RKA+KD++IKGYLIPKGW VL  F SVH+D++ 
Sbjct: 369 TDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDI 428

Query: 396 YDCPYKFNPWRWQDREMSSCN----FTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWI 455
           YD PY+F+PWRW DR   S N    FTPFGGGQRLCPGL+L++LE SIFLHH VT + W 
Sbjct: 429 YDNPYQFDPWRW-DRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWT 488

Query: 456 AEEDKIINFPTVRMKKRMPVWV 469
           AEED+I++FPTV+MK+R+P+ V
Sbjct: 489 AEEDEIVSFPTVKMKRRLPIRV 509

BLAST of CSPI07G22920 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.3e-103
Identity = 193/442 (43.67%), Postives = 289/442 (65.38%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I +  ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMAL 215
             V+ ++ +W+    + L +E K I+F++ VK L+S +PG+  E L+K++   I G  +L
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTV--PKDIMEVLLKDENEELTDDL 275
           P+ +  +   +++QA+R + + +  ++ +R+          KD++  LL   ++  +D+ 
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLA-ADDGFSDEE 270

Query: 276 IAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDY 335
           I + ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++  K     +L WSDY
Sbjct: 271 IVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSDY 330

Query: 336 LSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDC 395
            SMPFTQ V+ ETLR+ NII GV R+AM D++IKGY IPKGW V + FR+VHLD NH+  
Sbjct: 331 KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKD 390

Query: 396 PYKFNPWRWQDREMS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWI-AEE 455
              FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ AE+
Sbjct: 391 ARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQ 450

Query: 456 DKIINFPTVRMKKRMPVWVKRR 472
           DK++ FPT R +KR P++VKRR
Sbjct: 451 DKLVFFPTTRTQKRYPIFVKRR 468

BLAST of CSPI07G22920 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 330.1 bits (845), Expect = 4.1e-89
Identity = 173/474 (36.50%), Postives = 285/474 (60.13%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP G  GWPF+GETI ++    +     FM +    YGK+++S++FG PTIVS DA +++
Sbjct: 39  LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNR 98

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
           FILQ++ + F  SYP+S+  ++GK S+L++ G + + +  +   F   + L+  + KD+E
Sbjct: 99  FILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVE 158

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPG-QSMEFLKKQFQEFIAGLMA 215
            +    + +W +N+    QDE K  +F ++ K ++S++PG +  E LKK++  F+ G+++
Sbjct: 159 RHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVS 218

Query: 216 LPVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTVPKDIMEVLLKDENE------- 275
            P+N+PG+  +++LQ++  ++K +++ ++ERK   I    ++  EV  +DE E       
Sbjct: 219 APLNLPGTAYHKALQSRATILKFIERKMEERK-LDIKEEDQEEEEVKTEDEAEMSKSDHV 278

Query: 276 --ELTDD-----------LIAENMIDMMI----PGEDSVPILMTLAVKYLSDCPAALQQL 335
             + TDD           L  E ++D+++     G ++  + + LA+ +L  CP A+++L
Sbjct: 279 RKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEEL 338

Query: 336 TEENLKMKGQKEQLGEA-LHWSDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGY 395
            EE+L++   K++LGE+ L+W DY  M FTQ VI ETLR+GN++  + RKA+KD++ KGY
Sbjct: 339 REEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGY 398

Query: 396 LIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSC------------NFTPFGG 455
            IP GW VL    +VHLD++ YD P  FNPWRWQ +   +             N+ PFGG
Sbjct: 399 DIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPFGG 458

Query: 456 GQRLCPGLDLARLEASIFLHHFVTHFRW-IAEEDKIINFPTVRMKKRMPVWVKR 471
           G RLC G +LA+LE ++F+HH V  F W +AE+DK   FP V     +P+ V R
Sbjct: 459 GPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPFVDFPNGLPIRVSR 511

BLAST of CSPI07G22920 vs. ExPASy TrEMBL
Match: A0A0A0K7W8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1)

HSP 1 Score: 958.0 bits (2475), Expect = 1.5e-275
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 475
           LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 474

BLAST of CSPI07G22920 vs. ExPASy TrEMBL
Match: A0A1S3CGU6 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103500734 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.1e-269
Identity = 465/474 (98.10%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMA+WTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKD+MEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQ+RE+SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 475
           LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 471

BLAST of CSPI07G22920 vs. ExPASy TrEMBL
Match: E5GCA5 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 9.7e-251
Identity = 440/466 (94.42%), Postives = 449/466 (96.35%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMA+WTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKD+MEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQ+RE+SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPV 467
           LCPGLDLARLEASIFLHHFVTHFR+    D+  + P+VR     P+
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 462

BLAST of CSPI07G22920 vs. ExPASy TrEMBL
Match: A0A5A7UW93 (Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001660 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 3.7e-250
Identity = 441/470 (93.83%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMA+WTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQ----AKRNMVK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQ    AKRNMVK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASWTAKRNMVK 240

Query: 241 LVKKIIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLA 300
           LVKKIIQERKSCGISTVPKD+MEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLA
Sbjct: 241 LVKKIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLA 300

Query: 301 VKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGV 360
           VKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGV
Sbjct: 301 VKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGV 360

Query: 361 MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFG 420
           MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQ+RE+SSCNFTPFG
Sbjct: 361 MRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFG 420

Query: 421 GGQRLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPV 467
           GGQRLCPGLDLARLEASIFLHHFVTHFR+    D+  + P+VR     P+
Sbjct: 421 GGQRLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 466

BLAST of CSPI07G22920 vs. ExPASy TrEMBL
Match: A0A6P3YVG2 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Ziziphus jujuba OX=326968 GN=LOC107403831 PE=3 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 2.3e-220
Identity = 377/477 (79.04%), Postives = 427/477 (89.52%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTII--LYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYS 60
           MD LW +L VTA+F + II  LY+N +R         LPLG+LGWPF+GETIEF+S AYS
Sbjct: 1   MDKLW-ILQVTAVFLSAIILLLYKNSYRFRSKHRRKPLPLGTLGWPFLGETIEFVSCAYS 60

Query: 61  DRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMG 120
           DRPE+FMDKRR +YGKVFKSHIFGSPTIVSTDAEVSKF+LQSDAKAFVPSYPKSLTELMG
Sbjct: 61  DRPESFMDKRRSMYGKVFKSHIFGSPTIVSTDAEVSKFVLQSDAKAFVPSYPKSLTELMG 120

Query: 121 KSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETK 180
           KSSILLINGSLQ+RIHGLVGAFFKS HLKAQIT+DM+ YV+ SMANW+E++P Y+QD TK
Sbjct: 121 KSSILLINGSLQRRIHGLVGAFFKSPHLKAQITRDMQKYVQESMANWSEDHPTYIQDVTK 180

Query: 181 NISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLV 240
            I+FQVLVK LISLNPG  MEFLKKQFQEFIAGLM+LP+NIPGS LY+SLQAK+ MVKLV
Sbjct: 181 TIAFQVLVKALISLNPGDEMEFLKKQFQEFIAGLMSLPINIPGSTLYRSLQAKKKMVKLV 240

Query: 241 KKIIQERK-SCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAV 300
           KK IQ RK + GIS  PKD+++VLL D +E+LTDDLIA+NMIDMMIPGEDSVP+LMTLA+
Sbjct: 241 KKTIQARKNTSGISNFPKDVVDVLLSDTSEQLTDDLIADNMIDMMIPGEDSVPVLMTLAI 300

Query: 301 KYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVM 360
           KYLSDCPAALQQL EEN+K+K  K+QLGE L WSDYLS+PFTQ+VITETLRMGNIIIGVM
Sbjct: 301 KYLSDCPAALQQLMEENMKLKRHKDQLGEPLCWSDYLSLPFTQNVITETLRMGNIIIGVM 360

Query: 361 RKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGG 420
           RKAMKD++IKGYLIPKGW V  YFRSVHLD+NHYD PY+FNPWRWQD++MS+CNFTPFGG
Sbjct: 361 RKAMKDVEIKGYLIPKGWCVFAYFRSVHLDENHYDWPYEFNPWRWQDKDMSTCNFTPFGG 420

Query: 421 GQRLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 475
           GQRLCPGLDLARLEASIFLHHFVT FRW+AE+D I+NFPTVRMK+RMP+WVKRR DP
Sbjct: 421 GQRLCPGLDLARLEASIFLHHFVTQFRWVAEDDTIVNFPTVRMKRRMPIWVKRRGDP 476

BLAST of CSPI07G22920 vs. NCBI nr
Match: XP_008462361.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 936.0 bits (2418), Expect = 1.3e-268
Identity = 465/474 (98.10%), Postives = 469/474 (98.95%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMA+WTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKD+MEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQ+RE+SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 475
           LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 471

BLAST of CSPI07G22920 vs. NCBI nr
Match: XP_011659645.1 (LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus])

HSP 1 Score: 897.9 bits (2319), Expect = 3.8e-257
Identity = 450/474 (94.94%), Postives = 456/474 (96.20%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVWVKRRRDP 475
           LCPGLDLARLEASIFLHHFVTHFR+    D+  + P+VR     P+ +     P
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTXLPPLSLSPSSSP 473

BLAST of CSPI07G22920 vs. NCBI nr
Match: KAE8646515.1 (hypothetical protein Csa_016429 [Cucumis sativus])

HSP 1 Score: 894.4 bits (2310), Expect = 4.2e-256
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFR 445
           LCPGLDLARLEASIFLHHFVTHFR
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFR 444

BLAST of CSPI07G22920 vs. NCBI nr
Match: ADN34104.1 (cytochrome p450 [Cucumis melo subsp. melo])

HSP 1 Score: 875.5 bits (2261), Expect = 2.0e-250
Identity = 440/466 (94.42%), Postives = 449/466 (96.35%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAI   TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMA+WTENNPIYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGISTVPKD+MEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLI KGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQ+RE+SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPV 467
           LCPGLDLARLEASIFLHHFVTHFR+    D+  + P+VR     P+
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTNFPPL 462

BLAST of CSPI07G22920 vs. NCBI nr
Match: XP_038898401.1 (LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida])

HSP 1 Score: 874.8 bits (2259), Expect = 3.4e-250
Identity = 434/460 (94.35%), Postives = 447/460 (97.17%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60
           MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120
           PETFM+KRRRLYGKVFKSHIFGSPTIVSTD+EVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMNKRRRLYGKVFKSHIFGSPTIVSTDSEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180
           SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYV+LSMANWTENN IYLQDETKNI
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVQLSMANWTENNLIYLQDETKNI 180

Query: 181 SFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240
           SFQ+LVKILISLNPGQ MEFLKKQFQ+FIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK
Sbjct: 181 SFQILVKILISLNPGQRMEFLKKQFQDFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVKK 240

Query: 241 IIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300
           IIQERKSCGIS VPKD+MEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL
Sbjct: 241 IIQERKSCGISPVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKYL 300

Query: 301 SDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360
           SDCPAALQQLTEENLKMKG KEQLGE LHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA
Sbjct: 301 SDCPAALQQLTEENLKMKGLKEQLGETLHWSDYLSMPFTQSVITETLRMGNIIIGVMRKA 360

Query: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQR 420
           MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDR++SSCNFTPFGGGQR
Sbjct: 361 MKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDRDLSSCNFTPFGGGQR 420

Query: 421 LCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRM 461
           LCPGLDLARLEASIFLHHFVTHFR+    D+  + P+VR+
Sbjct: 421 LCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRL 459

BLAST of CSPI07G22920 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 613.2 bits (1580), Expect = 1.7e-175
Identity = 302/491 (61.51%), Postives = 382/491 (77.80%), Query Frame = 0

Query: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCN----------------QLPLGSLGWP 60
           MD+   +L  +  FF  I+++ N    LRS   +                + P GSLGWP
Sbjct: 1   MDTSSSLLFFSFFFFIIIVIF-NKINGLRSSPASKKKLNDHHVTSQSHGPKFPHGSLGWP 60

Query: 61  FIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKA 120
            IGETIEF+SSAYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV++ +LQSD+ A
Sbjct: 61  VIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRAVLQSDSTA 120

Query: 121 FVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMAN 180
           FVP YPK++ ELMGKSSILLINGSL +R HGLVG+F KS  LKAQI +DM  ++  SM  
Sbjct: 121 FVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHKFLSESMDL 180

Query: 181 WTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRL 240
           W+E+ P+ LQD +K ++F+VL K LIS+  G+ +E LK++F+ FI+GLM+LP+N PG++L
Sbjct: 181 WSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLPINFPGTQL 240

Query: 241 YQSLQAKRNMVKLVKKIIQ-----ERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMI 300
           ++SLQAK+NMVK V++II+      +       + KD+++VLLKD +E LT +LIA NMI
Sbjct: 241 HRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHLTHNLIANNMI 300

Query: 301 DMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFT 360
           DMMIPG DSVP+L+TLAVK+LSD PAAL  LTEEN+K+K  KE  GE L+W+DYLS+PFT
Sbjct: 301 DMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLYWNDYLSLPFT 360

Query: 361 QSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNP 420
           Q VITETLRMGN+IIGVMRKAMKD++IKGY+IPKGW  L Y RSVHLD  +Y+ PYKFNP
Sbjct: 361 QKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKLYYESPYKFNP 420

Query: 421 WRWQDREMSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVR 471
           WRWQ+R+M++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT FRWIAEED IINFPTV 
Sbjct: 421 WRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEEDTIINFPTVH 480

BLAST of CSPI07G22920 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.8e-149
Identity = 249/442 (56.33%), Postives = 344/442 (77.83%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           +P GSLGWP IGET+ FI+  YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
            +LQ+    FVP+YPKS+TEL+G++SIL ING  QKR+H L+GAF +S HLK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMAL 215
             V L++A+W +   +++QDE K ++F++LVK+L+S +PG+ M  LK +F+EFI GL+ +
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICI 248

Query: 216 PVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTVP-KDIMEVLLKD----ENEELT 275
           P+  PG+RLY+SL+AK  ++K+VKK+++ER+    +T P  D+++VLL+D    E +   
Sbjct: 249 PIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQP 308

Query: 276 DDLIAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHW 335
            D ++  +++MMIPGE+++P  MTLAVK+LSD P AL +L EEN++MK +K +LGE   W
Sbjct: 309 SDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKW 368

Query: 336 SDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNH 395
           +DY+S+ FTQ+VI ETLRM NII GV RKA+KD++IKGYLIPKGW VL  F SVH+D++ 
Sbjct: 369 TDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDI 428

Query: 396 YDCPYKFNPWRWQDREMSSCN----FTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWI 455
           YD PY+F+PWRW DR   S N    FTPFGGGQRLCPGL+L++LE SIFLHH VT + W 
Sbjct: 429 YDNPYQFDPWRW-DRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWT 488

Query: 456 AEEDKIINFPTVRMKKRMPVWV 469
           AEED+I++FPTV+MK+R+P+ V
Sbjct: 489 AEEDEIVSFPTVKMKRRLPIRV 509

BLAST of CSPI07G22920 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-104
Identity = 193/442 (43.67%), Postives = 289/442 (65.38%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I +  ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMAL 215
             V+ ++ +W+    + L +E K I+F++ VK L+S +PG+  E L+K++   I G  +L
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTV--PKDIMEVLLKDENEELTDDL 275
           P+ +  +   +++QA+R + + +  ++ +R+          KD++  LL   ++  +D+ 
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLA-ADDGFSDEE 270

Query: 276 IAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDY 335
           I + ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++  K     +L WSDY
Sbjct: 271 IVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSDY 330

Query: 336 LSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDC 395
            SMPFTQ V+ ETLR+ NII GV R+AM D++IKGY IPKGW V + FR+VHLD NH+  
Sbjct: 331 KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKD 390

Query: 396 PYKFNPWRWQDREMS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTHFRWI-AEE 455
              FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+ AE+
Sbjct: 391 ARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQ 450

Query: 456 DKIINFPTVRMKKRMPVWVKRR 472
           DK++ FPT R +KR P++VKRR
Sbjct: 451 DKLVFFPTTRTQKRYPIFVKRR 468

BLAST of CSPI07G22920 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 330.1 bits (845), Expect = 2.9e-90
Identity = 173/474 (36.50%), Postives = 285/474 (60.13%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP G  GWPF+GETI ++    +     FM +    YGK+++S++FG PTIVS DA +++
Sbjct: 39  LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNR 98

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
           FILQ++ + F  SYP+S+  ++GK S+L++ G + + +  +   F   + L+  + KD+E
Sbjct: 99  FILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVE 158

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPG-QSMEFLKKQFQEFIAGLMA 215
            +    + +W +N+    QDE K  +F ++ K ++S++PG +  E LKK++  F+ G+++
Sbjct: 159 RHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVS 218

Query: 216 LPVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTVPKDIMEVLLKDENE------- 275
            P+N+PG+  +++LQ++  ++K +++ ++ERK   I    ++  EV  +DE E       
Sbjct: 219 APLNLPGTAYHKALQSRATILKFIERKMEERK-LDIKEEDQEEEEVKTEDEAEMSKSDHV 278

Query: 276 --ELTDD-----------LIAENMIDMMI----PGEDSVPILMTLAVKYLSDCPAALQQL 335
             + TDD           L  E ++D+++     G ++  + + LA+ +L  CP A+++L
Sbjct: 279 RKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEEL 338

Query: 336 TEENLKMKGQKEQLGEA-LHWSDYLSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGY 395
            EE+L++   K++LGE+ L+W DY  M FTQ VI ETLR+GN++  + RKA+KD++ KGY
Sbjct: 339 REEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGY 398

Query: 396 LIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSC------------NFTPFGG 455
            IP GW VL    +VHLD++ YD P  FNPWRWQ +   +             N+ PFGG
Sbjct: 399 DIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPFGG 458

Query: 456 GQRLCPGLDLARLEASIFLHHFVTHFRW-IAEEDKIINFPTVRMKKRMPVWVKR 471
           G RLC G +LA+LE ++F+HH V  F W +AE+DK   FP V     +P+ V R
Sbjct: 459 GPRLCAGSELAKLEMAVFIHHLVLKFNWELAEDDKPFAFPFVDFPNGLPIRVSR 511

BLAST of CSPI07G22920 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 3.2e-81
Identity = 154/370 (41.62%), Postives = 238/370 (64.32%), Query Frame = 0

Query: 36  LPLGSLGWPFIGETIEFISSAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 95
           LP GSLG P IGET + I +  ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 96  FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLQKRIHGLVGAFFKSSHLKAQITKDME 155
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSL KR+H L  +F  SS +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 156 CYVKLSMANWTENNPIYLQDETKNISFQVLVKILISLNPGQSMEFLKKQFQEFIAGLMAL 215
             V+ ++ +W+    + L +E K I+F++ VK L+S +PG+  E L+K++   I G  +L
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFSL 210

Query: 216 PVNIPGSRLYQSLQAKRNMVKLVKKIIQERKSCGISTV--PKDIMEVLLKDENEELTDDL 275
           P+ +  +   +++QA+R + + +  ++ +R+          KD++  LL   ++  +D+ 
Sbjct: 211 PLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLA-ADDGFSDEE 270

Query: 276 IAENMIDMMIPGEDSVPILMTLAVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDY 335
           I + ++ +++ G ++   +MTLAVK+L++ P AL QL EE+ K++  K     +L WSDY
Sbjct: 271 IVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSDY 330

Query: 336 LSMPFTQSVITETLRMGNIIIGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDC 395
            SMPFTQ V+ ETLR+ NII GV R+AM D++IKGY IPKGW V + FR+VHLD NH+  
Sbjct: 331 KSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKD 390

Query: 396 PYKFNPWRWQ 404
              FNPWRWQ
Sbjct: 391 ARTFNPWRWQ 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94IA62.4e-17461.513-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q94IW52.0e-16862.36Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q9M0663.9e-14856.333-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q425692.3e-10343.67Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
O649894.1e-8936.50Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K7W81.5e-275100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1[more]
A0A1S3CGU66.1e-26998.103-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1035007... [more]
E5GCA59.7e-25194.42Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A5A7UW933.7e-25093.83Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G0016... [more]
A0A6P3YVG22.3e-22079.043-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Ziziphus jujuba OX=3269... [more]
Match NameE-valueIdentityDescription
XP_008462361.11.3e-26898.10PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo][more]
XP_011659645.13.8e-25794.94LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis... [more]
KAE8646515.14.2e-256100.00hypothetical protein Csa_016429 [Cucumis sativus][more]
ADN34104.12.0e-25094.42cytochrome p450 [Cucumis melo subsp. melo][more]
XP_038898401.13.4e-25094.35LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G13730.11.7e-17561.51cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT4G36380.12.8e-14956.33Cytochrome P450 superfamily protein [more]
AT5G05690.11.6e-10443.67Cytochrome P450 superfamily protein [more]
AT3G50660.12.9e-9036.50Cytochrome P450 superfamily protein [more]
AT5G05690.23.2e-8141.62Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 307..327
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 9..444
e-value: 1.1E-10
score: 38.9
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 15..466
NoneNo IPR availablePANTHERPTHR24286:SF303-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1coord: 15..466
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 412..422
score: 51.88
coord: 69..88
score: 25.43
coord: 380..404
score: 26.33
coord: 340..358
score: 24.05
coord: 422..445
score: 32.44
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 30..471
e-value: 1.8E-98
score: 332.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 35..471
IPR001128Cytochrome P450PFAMPF00067p450coord: 37..451
e-value: 7.5E-55
score: 186.5
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 415..424

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G22920.1CSPI07G22920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen