CSPI07G22520 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI07G22520
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description5'-phosphoribosylglycinamide transformylase
LocationChr7: 19097566 .. 19098444 (-)
RNA-Seq ExpressionCSPI07G22520
SyntenyCSPI07G22520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCCCAATGTTCTCTCTGTCGGTTTTGCCCAAAATTGTCTATTTCGTTGATTCGATCTCCTAATTATTCATTCCTTTTGAAGCTTCCTTCTTCTTCTTCTTCCTTTGTCCAAACCAGTCATTCCACTTTGCGTAGTGTTTCGTTCAAGGAGAGTATTGCTCGTAGAAAAAATGTTCAAATGGTTGTGAGTGCCAGTCTCAAAGGGGAAGTTTCAAAAACTGTGATTCAGAAGAAGAAACTTGCTGTTTTTGTCTCTGGTGGAGGCTCTAATTTTCGTTCAATACACGAGGCTTCTCTCGAAGGATCAGTTCACGGAGAAATTGTTGTTGTGGTTACTAACAAAAGTGGCTGTGGCGGTGCAGAATATGCAAGAAGTAAGAACATTCCAGTTGTCTTGTTTCCAAAAACCAAAGATGAACCCAATGGCTTATCTGCATCTGAACTTGTAGCTACCCTCAGACATTTTGAGATTGATTTCATTCTTCTTGCTGGATACCTAAAACTTATACCCCTTGAATTGATCCAAGCATATTCCAAATCTATAGTAAATATACACCCATCACTTCTTCCTGCTTTTGGAGGGAAAGGGTACTATGGAATGAAGGTGCACAAGGCAGTGGTTAACTCTGGAGCTCGGTTTTCAGGTGCAACAATTCATTTGGTTGATGAGCACTTTGACACTGGACGGATTCTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATTCAGCAGAGGATTTAGCAGCTAGGGTTCTTAAGGAGGAGCATGGCCTTTACGCTGAAGTAGTTGCAGCATTGTGCGACGAACGGATCATTTGGAGATCAGATGGTGTTCCTCTCATTCAAAGCAAAGAAAATCCTAATGAATTTTAC

mRNA sequence

ATGGAAGCCCAATGTTCTCTCTGTCGGTTTTGCCCAAAATTGTCTATTTCGTTGATTCGATCTCCTAATTATTCATTCCTTTTGAAGCTTCCTTCTTCTTCTTCTTCCTTTGTCCAAACCAGTCATTCCACTTTGCGTAGTGTTTCGTTCAAGGAGAGTATTGCTCGTAGAAAAAATGTTCAAATGGTTGTGAGTGCCAGTCTCAAAGGGGAAGTTTCAAAAACTGTGATTCAGAAGAAGAAACTTGCTGTTTTTGTCTCTGGTGGAGGCTCTAATTTTCGTTCAATACACGAGGCTTCTCTCGAAGGATCAGTTCACGGAGAAATTGTTGTTGTGGTTACTAACAAAAGTGGCTGTGGCGGTGCAGAATATGCAAGAAGTAAGAACATTCCAGTTGTCTTGTTTCCAAAAACCAAAGATGAACCCAATGGCTTATCTGCATCTGAACTTGTAGCTACCCTCAGACATTTTGAGATTGATTTCATTCTTCTTGCTGGATACCTAAAACTTATACCCCTTGAATTGATCCAAGCATATTCCAAATCTATAGTAAATATACACCCATCACTTCTTCCTGCTTTTGGAGGGAAAGGGTACTATGGAATGAAGGTGCACAAGGCAGTGGTTAACTCTGGAGCTCGGTTTTCAGGTGCAACAATTCATTTGGTTGATGAGCACTTTGACACTGGACGGATTCTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATTCAGCAGAGGATTTAGCAGCTAGGGTTCTTAAGGAGGAGCATGGCCTTTACGCTGAAGTAGTTGCAGCATTGTGCGACGAACGGATCATTTGGAGATCAGATGGTGTTCCTCTCATTCAAAGCAAAGAAAATCCTAATGAATTTTAC

Coding sequence (CDS)

ATGGAAGCCCAATGTTCTCTCTGTCGGTTTTGCCCAAAATTGTCTATTTCGTTGATTCGATCTCCTAATTATTCATTCCTTTTGAAGCTTCCTTCTTCTTCTTCTTCCTTTGTCCAAACCAGTCATTCCACTTTGCGTAGTGTTTCGTTCAAGGAGAGTATTGCTCGTAGAAAAAATGTTCAAATGGTTGTGAGTGCCAGTCTCAAAGGGGAAGTTTCAAAAACTGTGATTCAGAAGAAGAAACTTGCTGTTTTTGTCTCTGGTGGAGGCTCTAATTTTCGTTCAATACACGAGGCTTCTCTCGAAGGATCAGTTCACGGAGAAATTGTTGTTGTGGTTACTAACAAAAGTGGCTGTGGCGGTGCAGAATATGCAAGAAGTAAGAACATTCCAGTTGTCTTGTTTCCAAAAACCAAAGATGAACCCAATGGCTTATCTGCATCTGAACTTGTAGCTACCCTCAGACATTTTGAGATTGATTTCATTCTTCTTGCTGGATACCTAAAACTTATACCCCTTGAATTGATCCAAGCATATTCCAAATCTATAGTAAATATACACCCATCACTTCTTCCTGCTTTTGGAGGGAAAGGGTACTATGGAATGAAGGTGCACAAGGCAGTGGTTAACTCTGGAGCTCGGTTTTCAGGTGCAACAATTCATTTGGTTGATGAGCACTTTGACACTGGACGGATTCTTGCTCAAAGAGTGGTTCCTGTGCTTGCAAATGATTCAGCAGAGGATTTAGCAGCTAGGGTTCTTAAGGAGGAGCATGGCCTTTACGCTGAAGTAGTTGCAGCATTGTGCGACGAACGGATCATTTGGAGATCAGATGGTGTTCCTCTCATTCAAAGCAAAGAAAATCCTAATGAATTTTAC

Protein sequence

MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQTSHSTLRSVSFKESIARRKNVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY
Homology
BLAST of CSPI07G22520 vs. ExPASy Swiss-Prot
Match: P52423 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Vigna unguiculata OX=3917 GN=PUR3 PE=2 SV=2)

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-99
Identity = 197/314 (62.74%), Postives = 236/314 (75.16%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKL----SISLIRSPNYSFLLKLPS----SSSSFVQTSH-----STLRS 60
           MEAQ  + RFCPK     SI +++ P   F L  P     SS  F+Q+ +       L +
Sbjct: 1   MEAQQIISRFCPKSSLAPSIPMVKQP---FSLNFPPLHSLSSYPFLQSQNLGFPTGALHA 60

Query: 61  VSF--KESIARRKNVQMVVSASLKGEV-------SKTVIQKKKLAVFVSGGGSNFRSIHE 120
           +SF  KE  +    +    S+S   E        ++  +++KKLAVFVSGGGSNFRSIHE
Sbjct: 61  ISFVHKEVCSSSWRIWCSKSSSSTAEPEEDHEVRAQVTVRRKKLAVFVSGGGSNFRSIHE 120

Query: 121 ASLEGSVHGEIVVVVTNKSGCGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFE 180
           AS +GS+HG++ V+VTNKS CGGA+YAR+  IPV+LFPK KDEP GLS  +LV TLR FE
Sbjct: 121 ASKKGSLHGDVTVLVTNKSECGGAQYARNNGIPVILFPKAKDEPKGLSPCDLVDTLRKFE 180

Query: 181 IDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGA 240
           +DF+LLAGYLKLIP+ELI+A+ +SI NIHPSLLPAFGGKGYYGMKVHKAV+ SGARFSG 
Sbjct: 181 VDFVLLAGYLKLIPVELIRAFERSIFNIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGP 240

Query: 241 TIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSD 293
           TIH VDEH+DTGRILAQRVVPVLAND+AE+LAARVL EEH LY EVV ALC+ERI+WR D
Sbjct: 241 TIHFVDEHYDTGRILAQRVVPVLANDTAEELAARVLNEEHQLYVEVVEALCEERIVWRKD 300

BLAST of CSPI07G22520 vs. ExPASy Swiss-Prot
Match: P52422 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PUR3 PE=2 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 9.4e-84
Identity = 150/217 (69.12%), Postives = 183/217 (84.33%), Query Frame = 0

Query: 78  QKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCGGAEYARSKNIPVVLFPK 137
           ++KKLAVFVSGGGSNFR IHE   +GSV+G++V++VTNK  CGGAEYARS  IPV++FPK
Sbjct: 76  RRKKLAVFVSGGGSNFRKIHEGCSDGSVNGDVVLLVTNKKDCGGAEYARSNGIPVLVFPK 135

Query: 138 TKDEP-NGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGG 197
            K EP +GLS SELV  LR + +DF+LLAGYLKLIP+EL+QA+ K I+NIHP+LLPAFGG
Sbjct: 136 AKREPSDGLSPSELVDVLRKYGVDFVLLAGYLKLIPVELVQAFPKRILNIHPALLPAFGG 195

Query: 198 KGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKE 257
           KG YG+KVHKAV+ SGAR+SG TIH V+E +DTGRILAQ  V V+AND+ E+LA RVL E
Sbjct: 196 KGLYGIKVHKAVLESGARYSGPTIHFVNEEYDTGRILAQSAVRVIANDTPEELAKRVLHE 255

Query: 258 EHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           EH LY EVV A+C+ERI WR DGVPLIQ+K+NP+E+Y
Sbjct: 256 EHKLYVEVVGAICEERIKWREDGVPLIQNKQNPDEYY 292

BLAST of CSPI07G22520 vs. ExPASy Swiss-Prot
Match: Q42805 (Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Glycine max OX=3847 GN=PUR3 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-73
Identity = 155/286 (54.20%), Postives = 194/286 (67.83%), Query Frame = 0

Query: 12  PKLSISLIRSPNYSFLLKLPSSSSSFVQTSHSTLRSVSFKESIARRKNVQMVVSASLKGE 71
           P+L  S +   + S  L +PS +   +   H  + S S K       +            
Sbjct: 18  PRLPSSSLYPSSQSQNLNVPSGAFHPISIVHKEVCSSSCKRIWCSSSSSSTAEPKEGHEV 77

Query: 72  VSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCGGAEYARSKNIP 131
            ++  +++KKL VFVSGGG+NFR+IHEA+  GS+HG+++V+VTNKS CGGAEYAR+  IP
Sbjct: 78  RAQVTVRRKKLGVFVSGGGTNFRAIHEATKRGSLHGDVLVLVTNKSDCGGAEYARNNGIP 137

Query: 132 VVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLI-----PLELIQAYSKSIVNI 191
           V+L+  +KDE NG   S+LV TLR FE+DFILLAGYL L       L+ I     SI + 
Sbjct: 138 VILYHISKDESNG---SDLVDTLRKFEVDFILLAGYLNLYQWNDPSLQKIYIQHSSITS- 197

Query: 192 HPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPVLANDSA 251
                 +F  +G +GMKVHKAV+ SGARFSG TIH VDEH+DTGRILAQRVVPV AND+ 
Sbjct: 198 -----SSFWRQGIHGMKVHKAVIASGARFSGPTIHFVDEHYDTGRILAQRVVPVQANDTV 257

Query: 252 EDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEF 293
           E+LAARVLKEEH LY EVV ALC+ER++WR DGVPLIQSKENPNEF
Sbjct: 258 EELAARVLKEEHQLYVEVVEALCEERVVWRQDGVPLIQSKENPNEF 294

BLAST of CSPI07G22520 vs. ExPASy Swiss-Prot
Match: P00967 (Trifunctional purine biosynthetic protein adenosine-3 OS=Drosophila melanogaster OX=7227 GN=Gart PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.8e-26
Identity = 77/249 (30.92%), Postives = 131/249 (52.61%), Query Frame = 0

Query: 27   LLKLPSSSSSFVQTSHSTLRSVSFKESIARR--KNVQMVV---SASLKGEVSKTVIQKKK 86
            L+  P+     ++      R+    E +AR+  K  Q+VV    ASL         ++K+
Sbjct: 1096 LVVAPTEVEDVLKELRYPQRAAVVGEVVARKDPKKSQVVVQNFEASLARTQKMLSQRRKR 1155

Query: 87   LAVFVSGGGSNFRSIHEASLEGS--VHGEIVVVVTNKSGCGGAEYARSKNIPVVLFPKTK 146
            +AV +SG GSN +++ +A+ + +  +H ++V+V++NK G  G + A    IP ++     
Sbjct: 1156 VAVLISGTGSNLQALIDATRDSAQGIHADVVLVISNKPGVLGLQRATQAGIPSLVISHKD 1215

Query: 147  DEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKGY 206
                 +  +EL   L+   +D I LAG+++++    ++ +   +VNIHPSLLP      Y
Sbjct: 1216 FASREVYDAELTRNLKAARVDLICLAGFMRVLSAPFVREWRGRLVNIHPSLLPK-----Y 1275

Query: 207  YGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHG 266
             G+ V K  + +G + SG T+H VDE  DTG I+ Q  VP+L +D  + L  R+ K EH 
Sbjct: 1276 PGLHVQKQALEAGEKESGCTVHFVDEGVDTGAIIVQAAVPILPDDDEDSLTQRIHKAEHW 1335

Query: 267  LYAEVVAAL 269
             +   +A L
Sbjct: 1336 AFPRALAML 1339

BLAST of CSPI07G22520 vs. ExPASy Swiss-Prot
Match: P43846 (Phosphoribosylglycinamide formyltransferase OS=Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) OX=71421 GN=purN PE=3 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 5.2e-26
Identity = 68/215 (31.63%), Postives = 116/215 (53.95%), Query Frame = 0

Query: 80  KKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCGGAEYARSKNIPVVLFPKTK 139
           KK+AV +SG G+N ++I +A   G +  +I  V++NK+   G   A+   IP  +F + K
Sbjct: 2   KKIAVLISGQGTNLQTIIDACHSGDIPAKIACVISNKADAYGLVRAKQAQIPQAVFLR-K 61

Query: 140 DEPNGLSASELVAT-LRHFEIDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKG 199
           +  N L   + +   L+   +D I+LAGY+K++  +  Q ++  I+NIHPSLLP      
Sbjct: 62  NFSNNLEMDDAIGDYLQSLAVDLIVLAGYMKILTPKFTQRFAGKILNIHPSLLPK----- 121

Query: 200 YYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEH 259
           Y G+  ++  + +G    G T+H V+E  D G I+ Q  VP+   DS E++ AR  ++E+
Sbjct: 122 YAGLNTYQRAIEAGDNEHGTTVHFVNEEVDGGAIVLQAKVPIFPEDSIEEVEARTREQEY 181

Query: 260 GLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
            +Y  V+    + R+  + D +  +  K  P   Y
Sbjct: 182 QIYPLVIKWFTEGRLRLK-DNLAYLDGKALPKSGY 209

BLAST of CSPI07G22520 vs. ExPASy TrEMBL
Match: A0A0A0K7R9 (Formyl_trans_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450470 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.6e-155
Identity = 288/293 (98.29%), Postives = 292/293 (99.66%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQTSHSTLRSVSFKESIARRKNV 60
           MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQT+HSTLRSV FKE+IARRKNV
Sbjct: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQTNHSTLRSVWFKENIARRKNV 60

Query: 61  QMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCG 120
           QMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCG
Sbjct: 61  QMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCG 120

Query: 121 GAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAYS 180
           GAEYARSKNIPVVLFPKTKDEPNGLSASELVATL+HFEIDFILLAGYLKLIPLELIQAYS
Sbjct: 121 GAEYARSKNIPVVLFPKTKDEPNGLSASELVATLKHFEIDFILLAGYLKLIPLELIQAYS 180

Query: 181 KSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPV 240
           KSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPV
Sbjct: 181 KSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPV 240

Query: 241 LANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           LANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKE+PNEFY
Sbjct: 241 LANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKEHPNEFY 293

BLAST of CSPI07G22520 vs. ExPASy TrEMBL
Match: A0A1S3CGP8 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103500692 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 271/298 (90.94%), Postives = 278/298 (93.29%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLP-----SSSSSFVQTSHSTLRSVSFKESIA 60
           MEAQCSLCR CP  S  LIRSPNYSFLLKLP     SSSSSFVQT+HSTLRSV FKE+IA
Sbjct: 1   MEAQCSLCRSCPNFSFPLIRSPNYSFLLKLPSSSFFSSSSSFVQTNHSTLRSVWFKENIA 60

Query: 61  RRKNVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTN 120
            RK    VVSASL+GEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEG VHGEIVVVVTN
Sbjct: 61  CRK----VVSASLEGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGLVHGEIVVVVTN 120

Query: 121 KSGCGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180
           KS CGGAEYARSKNIPV+LFPKTKDEP+GLSASELVATLRHFEIDFILLAGYLKLIPLEL
Sbjct: 121 KSSCGGAEYARSKNIPVLLFPKTKDEPHGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180

Query: 181 IQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240
           IQAYSK I+NIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ
Sbjct: 181 IQAYSKCILNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240

Query: 241 RVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           RVVPVL+NDSAEDLAARVLKEEH LYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY
Sbjct: 241 RVVPVLSNDSAEDLAARVLKEEHRLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294

BLAST of CSPI07G22520 vs. ExPASy TrEMBL
Match: A0A6J1JYY4 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488685 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 9.6e-132
Identity = 245/295 (83.05%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSS--SFVQTSHSTLRSVSFKESIARRK 60
           MEAQCSL RFCP LSI L+ SP +  LLK PS SS  SFV+T+HST  SV FKE+IA RK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVLSPKHPILLKFPSYSSRPSFVRTNHSTSNSVWFKENIACRK 60

Query: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120
           N Q+V S+SL+G+VS+ VI+KKKLAVFVSGGGSNFRSIHEAS+EGS+HGEI VVVTNKSG
Sbjct: 61  NCQVVASSSLEGDVSRIVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPV+LFPKTKDEP+GLSAS+LVATLR FEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLP+FGGKGYYGMKVHKAV+ SGARFSG TIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPSFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           PVLAND+AEDLAARVLKEEH LY EVVAALCD+RI+WR DGVPLIQSK+NP+EFY
Sbjct: 241 PVLANDAAEDLAARVLKEEHHLYVEVVAALCDDRIVWREDGVPLIQSKDNPDEFY 295

BLAST of CSPI07G22520 vs. ExPASy TrEMBL
Match: A0A6J1GQR1 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456613 PE=3 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.8e-131
Identity = 245/295 (83.05%), Postives = 269/295 (91.19%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLP--SSSSSFVQTSHSTLRSVSFKESIARRK 60
           MEAQCSL RFCP LSI L+RSP +  LLK P  SS SSFV+T+HST  SV FKE+IA RK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKFPSYSSRSSFVRTNHSTSSSVWFKENIACRK 60

Query: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120
           N ++V SASL+G+VS+TVI+KKKLAVFVSGGGSNFRSIHEAS+EGS+HGEI VVVTNKS 
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSS 120

Query: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPV+LFPKTKDEP+GLSAS+LVATLR FEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLPAFGGKGYYGMKVHKAV+ SGARFSG TIHLVDE+FDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDENFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           PVLAND+AEDLAARVLKEEH LY EVVAALCD+RI+WR DGVPLIQSK+ P EFY
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPGEFY 295

BLAST of CSPI07G22520 vs. ExPASy TrEMBL
Match: A0A6J1DNE2 (phosphoribosylglycinamide formyltransferase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111022206 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 5.6e-124
Identity = 233/293 (79.52%), Postives = 260/293 (88.74%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSS-SFVQTSHSTLRSVSFKESIARRKN 60
           MEAQCS  RFCP LS+S  RSP  SFLLKLPS+SS SF ++S  T  SV  K+++  +KN
Sbjct: 1   MEAQCSFSRFCPNLSVSFFRSPKLSFLLKLPSASSCSFARSSVPTSPSVWLKKNVGVKKN 60

Query: 61  VQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGC 120
            QMVV  SL+GEVS+TVI++KKLAVFVSGGGSNFRSIHEASLEGS++G+IVV+VTNK  C
Sbjct: 61  SQMVVRVSLEGEVSRTVIRRKKLAVFVSGGGSNFRSIHEASLEGSINGDIVVLVTNKIDC 120

Query: 121 GGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAY 180
           GGAEYARSKNIPV+LFPKTKDEP+ LS S+LVA LR  EIDFILLAGYLKLIPLELIQAY
Sbjct: 121 GGAEYARSKNIPVILFPKTKDEPDRLSPSDLVANLRRLEIDFILLAGYLKLIPLELIQAY 180

Query: 181 SKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVP 240
           SKSI+NIHPSLLPAFGGKGYYGMKVHK+V+ SGAR+SG TIH VDEHFDTGRILAQRVVP
Sbjct: 181 SKSILNIHPSLLPAFGGKGYYGMKVHKSVIASGARYSGPTIHFVDEHFDTGRILAQRVVP 240

Query: 241 VLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEF 293
           VLAND+A+DLAARVLKEEH LY EVVAALC+ERI+WR DGVPLIQSKENPNEF
Sbjct: 241 VLANDAAQDLAARVLKEEHCLYVEVVAALCEERIVWREDGVPLIQSKENPNEF 293

BLAST of CSPI07G22520 vs. NCBI nr
Match: XP_004141701.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis sativus])

HSP 1 Score: 558.5 bits (1438), Expect = 3.4e-155
Identity = 288/293 (98.29%), Postives = 292/293 (99.66%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQTSHSTLRSVSFKESIARRKNV 60
           MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQT+HSTLRSV FKE+IARRKNV
Sbjct: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSSSFVQTNHSTLRSVWFKENIARRKNV 60

Query: 61  QMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCG 120
           QMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCG
Sbjct: 61  QMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCG 120

Query: 121 GAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAYS 180
           GAEYARSKNIPVVLFPKTKDEPNGLSASELVATL+HFEIDFILLAGYLKLIPLELIQAYS
Sbjct: 121 GAEYARSKNIPVVLFPKTKDEPNGLSASELVATLKHFEIDFILLAGYLKLIPLELIQAYS 180

Query: 181 KSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPV 240
           KSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPV
Sbjct: 181 KSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPV 240

Query: 241 LANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           LANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKE+PNEFY
Sbjct: 241 LANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKEHPNEFY 293

BLAST of CSPI07G22520 vs. NCBI nr
Match: XP_008462305.1 (PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo] >XP_008462306.1 PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo] >XP_008462307.1 PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo] >XP_008462308.1 PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis melo])

HSP 1 Score: 512.7 bits (1319), Expect = 2.1e-141
Identity = 271/298 (90.94%), Postives = 278/298 (93.29%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLP-----SSSSSFVQTSHSTLRSVSFKESIA 60
           MEAQCSLCR CP  S  LIRSPNYSFLLKLP     SSSSSFVQT+HSTLRSV FKE+IA
Sbjct: 1   MEAQCSLCRSCPNFSFPLIRSPNYSFLLKLPSSSFFSSSSSFVQTNHSTLRSVWFKENIA 60

Query: 61  RRKNVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTN 120
            RK    VVSASL+GEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEG VHGEIVVVVTN
Sbjct: 61  CRK----VVSASLEGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGLVHGEIVVVVTN 120

Query: 121 KSGCGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180
           KS CGGAEYARSKNIPV+LFPKTKDEP+GLSASELVATLRHFEIDFILLAGYLKLIPLEL
Sbjct: 121 KSSCGGAEYARSKNIPVLLFPKTKDEPHGLSASELVATLRHFEIDFILLAGYLKLIPLEL 180

Query: 181 IQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240
           IQAYSK I+NIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ
Sbjct: 181 IQAYSKCILNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQ 240

Query: 241 RVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           RVVPVL+NDSAEDLAARVLKEEH LYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY
Sbjct: 241 RVVPVLSNDSAEDLAARVLKEEHRLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294

BLAST of CSPI07G22520 vs. NCBI nr
Match: XP_023547957.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.6 bits (1241), Expect = 2.3e-132
Identity = 247/295 (83.73%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLP--SSSSSFVQTSHSTLRSVSFKESIARRK 60
           MEAQCSL RFCP LSI L+RSP +  LLK P  SS SSFV+T+HST  SV FKE+IA RK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVRSPKHPILLKCPSYSSRSSFVRTNHSTSSSVWFKETIACRK 60

Query: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120
           N ++V SASL+G+VS+TVI+KKKLAVFVSGGGSNFRSIHEAS+EGS+HGEI VVVTNKSG
Sbjct: 61  NCEIVASASLEGDVSRTVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPV+LFPKTKDEP+GLSAS+LVATLR FEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLPAFGGKGYYGMKVHKAV+ SGARFSG TIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPAFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           PVLAND+AEDLAARVLKEEH LY EVVAALCD+RI+WR DGVPLIQSK+ P EFY
Sbjct: 241 PVLANDAAEDLAARVLKEEHRLYVEVVAALCDDRIVWREDGVPLIQSKDKPGEFY 295

BLAST of CSPI07G22520 vs. NCBI nr
Match: XP_038896301.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 481.9 bits (1239), Expect = 4.0e-132
Identity = 250/295 (84.75%), Postives = 271/295 (91.86%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSS--SSSFVQTSHSTLRSVSFKESIARRK 60
           ME Q SL R CP  S+SLIR PN+SFL KLPS   S SFVQT HSTL SV F+++I  RK
Sbjct: 1   MEVQYSLYRVCPTPSVSLIRFPNHSFLSKLPSPCYSRSFVQTHHSTLHSVCFRKNITCRK 60

Query: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120
           N QM+V+ASL+GEVS+TVI+KKKLAVFVSGGGSNFRSI+EASLEGS+HGEIVVVVTNKSG
Sbjct: 61  NGQMIVNASLEGEVSRTVIRKKKLAVFVSGGGSNFRSINEASLEGSIHGEIVVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSK+IPV+LFPKTKDEP+GLSAS+LVATL  FEIDF+LLAGYLKLIP ELIQA
Sbjct: 121 CGGAEYARSKSIPVILFPKTKDEPHGLSASDLVATLGSFEIDFVLLAGYLKLIPPELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240
           YSKSIVNIHPSLLPAFGGKGYYGMKVHKAV++SGARFSGATIHLVDEHFDTGRILAQRVV
Sbjct: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVIDSGARFSGATIHLVDEHFDTGRILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           PVLANDSAEDLAAR+LKEEH LY EVVAALCDERIIWR+DGVPLIQSKENPNE Y
Sbjct: 241 PVLANDSAEDLAARILKEEHRLYVEVVAALCDERIIWRADGVPLIQSKENPNELY 295

BLAST of CSPI07G22520 vs. NCBI nr
Match: XP_022992348.1 (phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 479.6 bits (1233), Expect = 2.0e-131
Identity = 245/295 (83.05%), Postives = 270/295 (91.53%), Query Frame = 0

Query: 1   MEAQCSLCRFCPKLSISLIRSPNYSFLLKLPSSSS--SFVQTSHSTLRSVSFKESIARRK 60
           MEAQCSL RFCP LSI L+ SP +  LLK PS SS  SFV+T+HST  SV FKE+IA RK
Sbjct: 1   MEAQCSLSRFCPNLSIPLVLSPKHPILLKFPSYSSRPSFVRTNHSTSNSVWFKENIACRK 60

Query: 61  NVQMVVSASLKGEVSKTVIQKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSG 120
           N Q+V S+SL+G+VS+ VI+KKKLAVFVSGGGSNFRSIHEAS+EGS+HGEI VVVTNKSG
Sbjct: 61  NCQVVASSSLEGDVSRIVIRKKKLAVFVSGGGSNFRSIHEASVEGSIHGEIGVVVTNKSG 120

Query: 121 CGGAEYARSKNIPVVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQA 180
           CGGAEYARSKNIPV+LFPKTKDEP+GLSAS+LVATLR FEIDFILLAGYLKLIPLELIQA
Sbjct: 121 CGGAEYARSKNIPVILFPKTKDEPHGLSASDLVATLRSFEIDFILLAGYLKLIPLELIQA 180

Query: 181 YSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVV 240
           YSKSI+NIHPSLLP+FGGKGYYGMKVHKAV+ SGARFSG TIHLVDEHFDTG+ILAQRVV
Sbjct: 181 YSKSIINIHPSLLPSFGGKGYYGMKVHKAVIASGARFSGPTIHLVDEHFDTGKILAQRVV 240

Query: 241 PVLANDSAEDLAARVLKEEHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           PVLAND+AEDLAARVLKEEH LY EVVAALCD+RI+WR DGVPLIQSK+NP+EFY
Sbjct: 241 PVLANDAAEDLAARVLKEEHHLYVEVVAALCDDRIVWREDGVPLIQSKDNPDEFY 295

BLAST of CSPI07G22520 vs. TAIR 10
Match: AT1G31220.1 (Formyl transferase )

HSP 1 Score: 311.6 bits (797), Expect = 6.7e-85
Identity = 150/217 (69.12%), Postives = 183/217 (84.33%), Query Frame = 0

Query: 78  QKKKLAVFVSGGGSNFRSIHEASLEGSVHGEIVVVVTNKSGCGGAEYARSKNIPVVLFPK 137
           ++KKLAVFVSGGGSNFR IHE   +GSV+G++V++VTNK  CGGAEYARS  IPV++FPK
Sbjct: 76  RRKKLAVFVSGGGSNFRKIHEGCSDGSVNGDVVLLVTNKKDCGGAEYARSNGIPVLVFPK 135

Query: 138 TKDEP-NGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGG 197
            K EP +GLS SELV  LR + +DF+LLAGYLKLIP+EL+QA+ K I+NIHP+LLPAFGG
Sbjct: 136 AKREPSDGLSPSELVDVLRKYGVDFVLLAGYLKLIPVELVQAFPKRILNIHPALLPAFGG 195

Query: 198 KGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKE 257
           KG YG+KVHKAV+ SGAR+SG TIH V+E +DTGRILAQ  V V+AND+ E+LA RVL E
Sbjct: 196 KGLYGIKVHKAVLESGARYSGPTIHFVNEEYDTGRILAQSAVRVIANDTPEELAKRVLHE 255

Query: 258 EHGLYAEVVAALCDERIIWRSDGVPLIQSKENPNEFY 294
           EH LY EVV A+C+ERI WR DGVPLIQ+K+NP+E+Y
Sbjct: 256 EHKLYVEVVGAICEERIKWREDGVPLIQNKQNPDEYY 292

BLAST of CSPI07G22520 vs. TAIR 10
Match: AT5G47435.2 (formyltetrahydrofolate deformylase, putative )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 47/176 (26.70%), Postives = 77/176 (43.75%), Query Frame = 0

Query: 102 EGSVHGEIVVVVTNKSGCGGAEYAR---SKNIPVVLFPKTKDEPNGLSASELVATLRHFE 161
           +G +  +I  V++N          R      IP      TK+        ELV      +
Sbjct: 148 DGKLPVDITCVISNHERASNTHVMRFLERHGIPYHYVSTTKENKREDDILELVK-----D 207

Query: 162 IDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGA 221
            DF++LA Y++++    ++ Y K ++NIH  LLP+F G GY      K   ++G +  GA
Sbjct: 208 TDFLVLARYMQILSGNFLKGYGKDVINIHHGLLPSFKG-GYPA----KQAFDAGVKLIGA 267

Query: 222 TIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERII 275
           T H V E  D+G I+ Q V  V   D+      +    E       + + C+ R++
Sbjct: 268 TSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQKSEDLEKKCLTRAIKSYCELRVL 313

BLAST of CSPI07G22520 vs. TAIR 10
Match: AT5G47435.1 (formyltetrahydrofolate deformylase, putative )

HSP 1 Score: 67.8 bits (164), Expect = 1.7e-11
Identity = 47/176 (26.70%), Postives = 77/176 (43.75%), Query Frame = 0

Query: 102 EGSVHGEIVVVVTNKSGCGGAEYAR---SKNIPVVLFPKTKDEPNGLSASELVATLRHFE 161
           +G +  +I  V++N          R      IP      TK+        ELV      +
Sbjct: 148 DGKLPVDITCVISNHERASNTHVMRFLERHGIPYHYVSTTKENKREDDILELVK-----D 207

Query: 162 IDFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGA 221
            DF++LA Y++++    ++ Y K ++NIH  LLP+F G GY      K   ++G +  GA
Sbjct: 208 TDFLVLARYMQILSGNFLKGYGKDVINIHHGLLPSFKG-GYPA----KQAFDAGVKLIGA 267

Query: 222 TIHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERII 275
           T H V E  D+G I+ Q V  V   D+      +    E       + + C+ R++
Sbjct: 268 TSHFVTEELDSGPIIEQMVESVSHRDNLRSFVQKSEDLEKKCLTRAIKSYCELRVL 313

BLAST of CSPI07G22520 vs. TAIR 10
Match: AT4G17360.1 (Formyl transferase )

HSP 1 Score: 63.9 bits (154), Expect = 2.4e-10
Identity = 34/115 (29.57%), Postives = 58/115 (50.43%), Query Frame = 0

Query: 160 DFILLAGYLKLIPLELIQAYSKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGAT 219
           DF++LA Y++L+    ++ Y K ++NIH  LLP+F G+        K   ++G +  GAT
Sbjct: 209 DFLVLARYMQLLSGNFLKGYGKDVINIHHGLLPSFKGRNPV-----KQAFDAGVKLIGAT 268

Query: 220 IHLVDEHFDTGRILAQRVVPVLANDSAEDLAARVLKEEHGLYAEVVAALCDERII 275
            H V E  D+G I+ Q V  V   D+      +    E     + + + C+ R++
Sbjct: 269 THFVTEELDSGPIIEQMVERVSHRDNLRSFVQKSEDLEKKCLMKAIKSYCELRVL 318

BLAST of CSPI07G22520 vs. TAIR 10
Match: AT1G66520.1 (formyltransferase, putative )

HSP 1 Score: 44.7 bits (104), Expect = 1.5e-04
Identity = 36/151 (23.84%), Postives = 64/151 (42.38%), Query Frame = 0

Query: 122 AEYARSKNIP--VVLFPKTKDEPNGLSASELVATLRHFEIDFILLAGYLKLIPLELIQAY 181
           A+YA  K +P  ++  P+   +   LSA      LR  + +  + A Y  ++P + ++  
Sbjct: 79  AQYALDKGLPSDLIFSPEKAGDEAFLSA------LRELQPELCITAAYGNILPTKFLKIP 138

Query: 182 SKSIVNIHPSLLPAFGGKGYYGMKVHKAVVNSGARFSGATIHLVDEHFDTGRILAQRVVP 241
               VNIHPSLLP      Y G    +  +  G   +G ++       D G ++A +   
Sbjct: 139 VHGTVNIHPSLLPL-----YRGAAPVQRALQDGVPETGVSLAFTVRKLDAGPVIASKRFQ 198

Query: 242 VLANDSAEDLAARVLKEEHGLYAEVVAALCD 271
           V     A +L + +  E   L    + ++ D
Sbjct: 199 VDDLIKAPELLSFLFSEGSNLLIRELPSIFD 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P524231.6e-9962.74Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Vigna unguiculata ... [more]
P524229.4e-8469.12Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Arabidopsis thalia... [more]
Q428051.5e-7354.20Phosphoribosylglycinamide formyltransferase, chloroplastic OS=Glycine max OX=384... [more]
P009671.8e-2630.92Trifunctional purine biosynthetic protein adenosine-3 OS=Drosophila melanogaster... [more]
P438465.2e-2631.63Phosphoribosylglycinamide formyltransferase OS=Haemophilus influenzae (strain AT... [more]
Match NameE-valueIdentityDescription
A0A0A0K7R91.6e-15598.29Formyl_trans_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G450... [more]
A0A1S3CGP81.0e-14190.94phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucumis melo OX=36... [more]
A0A6J1JYY49.6e-13283.05phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita maxima O... [more]
A0A6J1GQR12.8e-13183.05phosphoribosylglycinamide formyltransferase, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1DNE25.6e-12479.52phosphoribosylglycinamide formyltransferase, chloroplastic OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
XP_004141701.13.4e-15598.29phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis sativus][more]
XP_008462305.12.1e-14190.94PREDICTED: phosphoribosylglycinamide formyltransferase, chloroplastic [Cucumis m... [more]
XP_023547957.12.3e-13283.73phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita pepo subsp... [more]
XP_038896301.14.0e-13284.75phosphoribosylglycinamide formyltransferase, chloroplastic [Benincasa hispida][more]
XP_022992348.12.0e-13183.05phosphoribosylglycinamide formyltransferase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G31220.16.7e-8569.12Formyl transferase [more]
AT5G47435.21.7e-1126.70formyltetrahydrofolate deformylase, putative [more]
AT5G47435.11.7e-1126.70formyltetrahydrofolate deformylase, putative [more]
AT4G17360.12.4e-1029.57Formyl transferase [more]
AT1G66520.11.5e-0423.84formyltransferase, putative [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.170coord: 60..291
e-value: 6.4E-64
score: 217.1
NoneNo IPR availablePIRSFPIRSF036480FormyFH4_hydrcoord: 26..285
e-value: 1.1E-56
score: 190.1
NoneNo IPR availablePANTHERPTHR43369PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASEcoord: 54..286
IPR004607Phosphoribosylglycinamide formyltransferaseTIGRFAMTIGR00639TIGR00639coord: 80..273
e-value: 1.7E-48
score: 162.7
IPR004607Phosphoribosylglycinamide formyltransferaseHAMAPMF_01930PurNcoord: 81..271
score: 26.781677
IPR004607Phosphoribosylglycinamide formyltransferaseCDDcd08645FMT_core_GARTcoord: 81..268
e-value: 1.12821E-82
score: 244.989
IPR002376Formyl transferase, N-terminalPFAMPF00551Formyl_trans_Ncoord: 80..265
e-value: 2.9E-55
score: 186.8
IPR036477Formyl transferase, N-terminal domain superfamilySUPERFAMILY53328Formyltransferasecoord: 80..282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI07G22520.1CSPI07G22520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006189 'de novo' IMP biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004644 phosphoribosylglycinamide formyltransferase activity
molecular_function GO:0016742 hydroxymethyl-, formyl- and related transferase activity