CSPI07G19300 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G19300
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	E3 ubiquitin ligase BIG BROTHER
Location	Chr7: 17017646 .. 17020514 (-)
RNA-Seq Expression	CSPI07G19300
Synteny	CSPI07G19300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTTTCTTGGGGAAGTTTCTGTTTGGCTATCAAGTAAGAAAAATCATAAGATTTTTCTTTGTGTTTTTCGTTTTGGAGACAAAAGAAGTTAAATGTAAGTTTTTCTTTTGGTTTTGTGTAATGTTCATGTTTGTTTTAAAGTTTTTTTTGAGTGTTTTTAGGGTCTGTTTCCTATTTCATTGAATAGAAAATTTCAAATCTTCAATTTCCTTTGCTTTCTCATCTTTTCCTCTAAGTTTTCTTATCAACCAAACAGATTGTTGTGGATAAGTTCATTCTAAAGCTTTGACAATGTAGGTTTTAAGTTCATTTCATATTTCATTTTGTTTGATATGTTATTTTCCTTTTGGTCTTTTTTGCAAATGTTACTGTGATTGTTGCTTAAGATGAATGAGGATAGACAAATGGAATTGCATTACATGGACACCGGCTTTCCTTACACGAATACAGAGAGCTTCATGGATTTCTTTCAAGGGCTTCACCAACATCAAGTGCCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGTAACAGAGCGGGTCATGAAAAACTGATGCAATTTATTTGTCAATGTTCGAGCTCGAGTTTATTCATTTGAGAGAATAATATTGGAAATGCACGGTGATTTGTTTCTCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACGTATTACGGCCATTCGGAGGAAAGTCACCATCTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGCGATGAATGTCGAAATCCCCGAACCATCCTATGCGCCATCCGTGGAGGAAGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTAAGTGTTGACAAATTTGATACCGAGTCTGTTTATTTCATACTTTTCAAAGCTAACAATGAGTTTGTCACTATCACATCGTCCTGTTGAATCGTTGGTACGTTTGGATCTTACCAGCATCTTGTTACCTTGTTTCCTGTTTTGCAGGTGATTTAAGCCATCGGGAGGAAACTAGTACGTCTCAAGTATGATGCTTTCTCCTTGATTTCGCAATATGCCTTATTTTTCTGTTATGACCATGATCTACTTAATGCTCCTTACGAACCGTGGCTTAGTAAAAAAATTATTTGGTATCCTTTCACGTAATTCAGTAAATAATGAACTAGGGTTTCATCTTATTATCATCAAAGGTTGTAGAATGTTCATCAAATGTTATTACTCTTAAAGTCTTAGTTTATGTCAGAAAGTTTCTCTTTCTGATCAGTTTTGTGCCCAACGAAGTTCCATTGCTTTTGAACTATGGGAATAGGTTCCTTTTTGATTTGGTAGGGCTCATTGTATCAGTCTTCTTTTGTTCGTCTCTTCAGGTTACATGGCAAGATGAGATTGATCCTGACCACATGACTTATGAGGTATGATTTCAACTCTATCATTTACAAACCAGATTTGTTTAAGAACATGTTTTAAGAAATGTTCTGTAAATCTTCAATAGATTGCCCTATAGAAGTTGAGGCTGTTCTTCACTTGAAACCAATGTTTTATTGAGCGCATGATTATTTTGGTTTAAAGAAAAAGATTTTAACTCAATTATCAACTAGCTTCTAGTTTCTTATGGCCATATGAACTATGGTTGGTTGTCAAATAAATGGGATCCACAGAAGTTTATTGTATAAAACAGAAGAGTATAAAAGGACTGCCTTTAGCCATGACTGTGCAGATGATCTTATATGGTTTTACAGGAACTGTTGGACTTGGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCACGATGCAAGTTAACGAGCTTTTTCTCAAGAAAAAAGCTCGATGAGAGGTCAGTTTTTGACAAATGTTACATTTCAGTTATCTTGCACTATCTTTGTTAATCTGCTTTACTTGGTGGGTATATGAGAAGACTCCTGTCAGAATAACTTTGAATCATCTGTCAATAATCGGTCTGGTGTCAATGTAGATGACAGTTTCTATAAACCGAGATGTATAATCGGTTTGGTGTCAATGTGTCTACACTGAAGTCACATGGATCTATTTTTCTGTCATAGTACACGTTAATACATTCACTTTGGTTTCTTTAGTTTTTAGTGTAGCAATGTCGAACTCGAACCTCTAACCAATGTTACGAATAAGATAGTCATATGAGACACAAGAAGATGTTTACATCTTGGTACTTTCTCGATACAGTATTCATTACTGGAATATTTGATTGTACTTGTTTTGTTTCAACTGCAGATGTGTGATTTGCCAAATGAGGTACAAGCGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTCTACCACAACAAATGCATAACCAAATGGCTCACCATCAACAAAGTCAGTAATTTTCTTGGCATTCTCATATCATACTTCCACCATTTCTGTTCTAACTTGATGTTCTACCTCGTCACTCACCATCGTTTTTATCAGATATGTCCAATCTGCAACATCGAGGTGTTCGGAGATGGAAGCACGAATGCATCGGCCAGTGATAACTGACAGTAGATTTTGGAAGCTCTAACATTTTGTTCTTGTTCATCTTTTCCCATTTTTGCCTTCTCACTTTCATTTCTCTCCACATTTTGTAAATCTCTACTTAACTAAGCGAATCAAGTACACTCTAATCCTTACTCTCTCCCTCCCCACGCTTGCCTTATGATCAACAGAAAGAAAGAAAATGTAACCCCTCTTCTTAACTTTTTAACTGCTTAGCCTTAAGATTGAAGTCTAGTTATTTTCTTAGATTAAGATTAGATTATTTTGTAGTCATTTTACGGACTGGGG

mRNA sequence

GAGTTTCTTGGGGAAGTTTCTGTTTGGCTATCAAGTAAGAAAAATCATAAGATTTTTCTTTGTGTTTTTCGTTTTGGAGACAAAAGAAGTTAAATATGAATGAGGATAGACAAATGGAATTGCATTACATGGACACCGGCTTTCCTTACACGAATACAGAGAGCTTCATGGATTTCTTTCAAGGGCTTCACCAACATCAAGTGCCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACGTATTACGGCCATTCGGAGGAAAGTCACCATCTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGCGATGAATGTCGAAATCCCCGAACCATCCTATGCGCCATCCGTGGAGGAAGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTGATTTAAGCCATCGGGAGGAAACTAGTACGTCTCAAGTTACATGGCAAGATGAGATTGATCCTGACCACATGACTTATGAGGAACTGTTGGACTTGGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCACGATGCAAGTTAACGAGCTTTTTCTCAAGAAAAAAGCTCGATGAGAGATGTGTGATTTGCCAAATGAGGTACAAGCGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTCTACCACAACAAATGCATAACCAAATGGCTCACCATCAACAAAATATGTCCAATCTGCAACATCGAGGTGTTCGGAGATGGAAGCACGAATGCATCGGCCAGTGATAACTGACAGTAGATTTTGGAAGCTCTAACATTTTGTTCTTGTTCATCTTTTCCCATTTTTGCCTTCTCACTTTCATTTCTCTCCACATTTTGTAAATCTCTACTTAACTAAGCGAATCAAGTACACTCTAATCCTTACTCTCTCCCTCCCCACGCTTGCCTTATGATCAACAGAAAGAAAGAAAATGTAACCCCTCTTCTTAACTTTTTAACTGCTTAGCCTTAAGATTGAAGTCTAGTTATTTTCTTAGATTAAGATTAGATTATTTTGTAGTCATTTTACGGACTGGGG

Coding sequence (CDS)

ATGAATGAGGATAGACAAATGGAATTGCATTACATGGACACCGGCTTTCCTTACACGAATACAGAGAGCTTCATGGATTTCTTTCAAGGGCTTCACCAACATCAAGTGCCTGCTTATGGCAATGCTGTACCATTGCTCGATCAGGGAAATGCTTACTGGTCAATGAATATGCAATGTTACAAATTTGGAGTATCTGATCATGGGAATACGTATTACGGCCATTCGGAGGAAAGTCACCATCTACCACCTTCAATGGATGTTGGTGAAAGGGAATGGGAGTATCCTACTTCAGCGATGAATGTCGAAATCCCCGAACCATCCTATGCGCCATCCGTGGAGGAAGATGTTGTAGATGCTCATTCTATACCAGAAGAATGTGATTTAAGCCATCGGGAGGAAACTAGTACGTCTCAAGTTACATGGCAAGATGAGATTGATCCTGACCACATGACTTATGAGGAACTGTTGGACTTGGGTGAATCAGTTGGAACTGAAAGTCGGGGTCTTTCTGAAGAACAAATAAGTTTGCTTCCGACTGCACGATGCAAGTTAACGAGCTTTTTCTCAAGAAAAAAGCTCGATGAGAGATGTGTGATTTGCCAAATGAGGTACAAGCGAGGGGACAAACAAATAAAATTACCCTGCAAGCATTTCTACCACAACAAATGCATAACCAAATGGCTCACCATCAACAAAATATGTCCAATCTGCAACATCGAGGTGTTCGGAGATGGAAGCACGAATGCATCGGCCAGTGATAACTGA

Protein sequence

MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGSTNASASDN*

Homology

[Close]

BLAST of CSPI07G19300 vs. ExPASy Swiss-Prot
Match: Q8L649 (E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 4.6e-63
Identity = 123/249 (49.40%), Postives = 166/249 (66.67%), Query Frame = 0


Query: 1   MNEDRQ--MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y       + PE + A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC +++  +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240
           PT + K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INK+CP+C
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVC 240

Query: 241 NIEVFGDGS 247
           N EVFG+ S
Sbjct: 241 NSEVFGEPS 246

[Close]

BLAST of CSPI07G19300 vs. ExPASy Swiss-Prot
Match: Q9LT17 (E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.9e-24
Identity = 82/234 (35.04%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 45  LLDQGNAYW--SMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS----- 104
           L +Q  AY   +MN +   +G  + G+  Y + E+    P + D  + E EY T      
Sbjct: 104 LQEQERAYMMLTMNSEISDYGSWETGS--YVYDEDEFDDPENEDEDDDEDEYETDDDPQE 163

Query: 105 ---AMNVEIPEPSYAPSVEEDVVD-----------AHSIPEECDLSHR------------ 164
               +NV   E         D+ +           A    EE D++ R            
Sbjct: 164 DGLDVNVHANEDDQEDDGNSDIEEVAYTDDEAYARALQEAEERDMAARLSALSGLANRVV 223

Query: 165 ----EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSF 224
               +E+ TSQ  W DE+DPD ++YEELL LG+ VGTESRGLS + I+ LP+ R K    
Sbjct: 224 EDLEDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD- 283

Query: 225 FSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEV 242
            ++   +E CVIC++ Y+  +  I LPCKH YH++CI  WL INK+CP+C+ EV
Sbjct: 284 -NQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of CSPI07G19300 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.5e-13
Identity = 43/99 (43.43%), Postives = 53/99 (53.54%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLD---ERCVICQ 204
           +D D+M+YEELL LGE +G  S GLSEE I          +S  S  +L    E C ICQ
Sbjct: 601 LDVDNMSYEELLALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQ 660

Query: 205 MRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 241
             Y  GD    L C H +H  CI +W+ I  +CPIC  E
Sbjct: 661 EEYVEGDNLGTLKCGHEFHKDCIKQWVMIKNLCPICKTE 699

[Close]

BLAST of CSPI07G19300 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.6e-13
Identity = 38/93 (40.86%), Postives = 51/93 (54.84%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDERCVICQMRY 204
           +D D+M+YEELL LGE +G  S GLSEE I  +       +S     +  E C +CQ  Y
Sbjct: 567 LDVDNMSYEELLALGERIGDVSTGLSEEVILKVMKQHKHTSSAAGSHQDMEPCCVCQEEY 626

Query: 205 KRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 238
             GD    L C H +H  C+ +WL +  +CPIC
Sbjct: 627 AEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 659

[Close]

BLAST of CSPI07G19300 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 4.4e-13
Identity = 40/94 (42.55%), Postives = 55/94 (58.51%), Query Frame = 0


Query: 145 IDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSFFSRKKLDER-CVICQMR 204
           +D D+M+YEELL L E +G  S GLSEE+++ L   R K +S+     ++E  C ICQ  
Sbjct: 568 LDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQR-KFSSWRLEASVEEEPCCICQEE 627

Query: 205 YKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 238
           Y  GD    L C H +H  C+ +WL +   CPIC
Sbjct: 628 YVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPIC 660

[Close]

BLAST of CSPI07G19300 vs. ExPASy TrEMBL
Match: A0A1S3CED1 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 7.5e-149
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSTNASASDN 255
           VFGDGSTNASASDN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of CSPI07G19300 vs. ExPASy TrEMBL
Match: A0A5D3CGQ8 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00890 PE=4 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 3.7e-148
Identity = 252/254 (99.21%), Postives = 253/254 (99.61%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSTNASASDN 255
           VFGDGSTNASASDN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of CSPI07G19300 vs. ExPASy TrEMBL
Match: A0A0A0K8C4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.0e-145
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 7   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 186
           DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 246
           FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 240

Query: 247 TNASASDN 255
           TNASASDN
Sbjct: 241 TNASASDN 248

[Close]

BLAST of CSPI07G19300 vs. ExPASy TrEMBL
Match: A0A1S3CES7 (E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC103499919 PE=4 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.0e-145
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 7   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 186
           DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 246
           FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 240

Query: 247 TNASASDN 255
           TNASASDN
Sbjct: 241 TNASASDN 248

[Close]

BLAST of CSPI07G19300 vs. ExPASy TrEMBL
Match: A0A5A7UTY6 (E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001030 PE=4 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.2e-139
Identity = 242/256 (94.53%), Postives = 245/256 (95.70%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECD--LSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP 180
           SIPEEC   +      S+ QV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP
Sbjct: 121 SIPEECKWLIVSVFFCSSLQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLP 180

Query: 181 TARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICN 240
           TARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICN
Sbjct: 181 TARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICN 240

Query: 241 IEVFGDGSTNASASDN 255
           IEVFGDGSTNASASDN
Sbjct: 241 IEVFGDGSTNASASDN 256

[Close]

BLAST of CSPI07G19300 vs. NCBI nr
Match: XP_011659453.1 (E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >XP_011659454.1 E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >KAE8646405.1 hypothetical protein Csa_015770 [Cucumis sativus])

HSP 1 Score: 537.7 bits (1384), Expect = 5.3e-149
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSTNASASDN 255
           VFGDGSTNASASDN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of CSPI07G19300 vs. NCBI nr
Match: XP_008461282.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_008461283.1 PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo])

HSP 1 Score: 536.2 bits (1380), Expect = 1.6e-148
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSTNASASDN 255
           VFGDGSTNASASDN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of CSPI07G19300 vs. NCBI nr
Match: TYK10442.1 (E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 533.9 bits (1374), Expect = 7.7e-148
Identity = 252/254 (99.21%), Postives = 253/254 (99.61%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSHREETSTSQV WQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHREETSTSQVIWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSTNASASDN 255
           VFGDGSTNASASDN
Sbjct: 241 VFGDGSTNASASDN 254

[Close]

BLAST of CSPI07G19300 vs. NCBI nr
Match: XP_008461284.1 (PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis melo])

HSP 1 Score: 524.2 bits (1349), Expect = 6.1e-145
Identity = 247/248 (99.60%), Postives = 248/248 (100.00%), Query Frame = 0


Query: 7   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 66
           MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD
Sbjct: 1   MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCYKFGVSD 60

Query: 67  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAHSIPEEC 126
           HGNTYYGHSEESHHLPPSMDVGEREWEYPTSA+NVEIPEPSYAPSVEEDVVDAHSIPEEC
Sbjct: 61  HGNTYYGHSEESHHLPPSMDVGEREWEYPTSAVNVEIPEPSYAPSVEEDVVDAHSIPEEC 120

Query: 127 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 186
           DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS
Sbjct: 121 DLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTS 180

Query: 187 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 246
           FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS
Sbjct: 181 FFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVFGDGS 240

Query: 247 TNASASDN 255
           TNASASDN
Sbjct: 241 TNASASDN 248

[Close]

BLAST of CSPI07G19300 vs. NCBI nr
Match: XP_038899262.1 (E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899264.1 E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida])

HSP 1 Score: 510.4 bits (1313), Expect = 9.1e-141
Identity = 240/254 (94.49%), Postives = 247/254 (97.24%), Query Frame = 0


Query: 1   MNEDRQMELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60
           MNEDRQME+HY+DTGFPYT TESFMDFF+GLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY
Sbjct: 1   MNEDRQMEVHYIDTGFPYTVTESFMDFFEGLHQHQVPAYGNAVPLLDQGNAYWSMNMQCY 60

Query: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVVDAH 120
           KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS  NVEIPEP+YAPSVEEDVVDAH
Sbjct: 61  KFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTSVANVEIPEPTYAPSVEEDVVDAH 120

Query: 121 SIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180
           SIPEECDLSH+EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA
Sbjct: 121 SIPEECDLSHQEETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTA 180

Query: 181 RCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240
           R KLTSFFSRKK +ERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE
Sbjct: 181 RFKLTSFFSRKKPEERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIE 240

Query: 241 VFGDGSTNASASDN 255
           VFGDGS NAS +DN
Sbjct: 241 VFGDGSMNASGNDN 254

[Close]

BLAST of CSPI07G19300 vs. TAIR 10
Match: AT3G63530.1 (RING/U-box superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 3.3e-64
Identity = 123/249 (49.40%), Postives = 166/249 (66.67%), Query Frame = 0


Query: 1   MNEDRQ--MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y       + PE + A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC +++  +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240
           PT + K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INK+CP+C
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVC 240

Query: 241 NIEVFGDGS 247
           N EVFG+ S
Sbjct: 241 NSEVFGEPS 246

[Close]

BLAST of CSPI07G19300 vs. TAIR 10
Match: AT3G63530.2 (RING/U-box superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 3.3e-64
Identity = 123/249 (49.40%), Postives = 166/249 (66.67%), Query Frame = 0


Query: 1   MNEDRQ--MELHYMDTGFPYTNTESFMDFFQGLHQHQVPAYGNAVPLLDQGNAYWSMNMQ 60
           MN D +   + HY +TGFPY  T S+MDF+ G  Q  +  Y +A  +  Q N YW+MN  
Sbjct: 1   MNGDNRPVEDAHYTETGFPYAATGSYMDFYGGAAQGPL-NYDHAATMHPQDNLYWTMNTN 60

Query: 61  CYKFGVSDHGN-TYYGHSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV 120
            YKFG S   N ++YG  + + HL   M +G   W+Y       + PE + A SV+    
Sbjct: 61  AYKFGFSGSDNASFYGSYDMNDHL-SRMSIGRTNWDYHPMVNVADDPENTVARSVQIGDT 120

Query: 121 DAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLL 180
           D HS  EEC +++  +  + QV+WQD+IDPD MTYEEL++LGE+VGTESRGLS+E I  L
Sbjct: 121 DEHSEAEEC-IANEHDPDSPQVSWQDDIDPDTMTYEELVELGEAVGTESRGLSQELIETL 180

Query: 181 PTARCKLTSFFSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPIC 240
           PT + K  S FSRK+  ERCVICQ++YK G++Q+ LPCKH YH++CI+KWL+INK+CP+C
Sbjct: 181 PTKKYKFGSIFSRKRAGERCVICQLKYKIGERQMNLPCKHVYHSECISKWLSINKVCPVC 240

Query: 241 NIEVFGDGS 247
           N EVFG+ S
Sbjct: 241 NSEVFGEPS 246

[Close]

BLAST of CSPI07G19300 vs. TAIR 10
Match: AT3G19910.1 (RING/U-box superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-25
Identity = 82/234 (35.04%), Postives = 118/234 (50.43%), Query Frame = 0


Query: 45  LLDQGNAYW--SMNMQCYKFGVSDHGNTYYGHSEESHHLPPSMDVGEREWEYPTS----- 104
           L +Q  AY   +MN +   +G  + G+  Y + E+    P + D  + E EY T      
Sbjct: 104 LQEQERAYMMLTMNSEISDYGSWETGS--YVYDEDEFDDPENEDEDDDEDEYETDDDPQE 163

Query: 105 ---AMNVEIPEPSYAPSVEEDVVD-----------AHSIPEECDLSHR------------ 164
               +NV   E         D+ +           A    EE D++ R            
Sbjct: 164 DGLDVNVHANEDDQEDDGNSDIEEVAYTDDEAYARALQEAEERDMAARLSALSGLANRVV 223

Query: 165 ----EETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTARCKLTSF 224
               +E+ TSQ  W DE+DPD ++YEELL LG+ VGTESRGLS + I+ LP+ R K    
Sbjct: 224 EDLEDESHTSQDAW-DEMDPDELSYEELLALGDIVGTESRGLSADTIASLPSKRYKEGD- 283

Query: 225 FSRKKLDERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEV 242
            ++   +E CVIC++ Y+  +  I LPCKH YH++CI  WL INK+CP+C+ EV
Sbjct: 284 -NQNGTNESCVICRLDYEDDEDLILLPCKHSYHSECINNWLKINKVCPVCSAEV 332

[Close]

BLAST of CSPI07G19300 vs. TAIR 10
Match: AT5G52140.1 (RING/U-box superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 5.9e-21
Identity = 63/180 (35.00%), Postives = 92/180 (51.11%), Query Frame = 0


Query: 74  HSEESHHLPPSMDVGEREWEYPTSAMNVEIPEPSYAPSVEEDVV---DAHSIPEECDLSH 133
           H E +++    +        +P       IP+   APS    +    D  +       +H
Sbjct: 101 HDELANNTEDDIQETHHHQYHPMQVNRSYIPQRD-APSSSRAIFHYDDGENFSGRYTHTH 160

Query: 134 REETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSEEQISLLPTAR--CKLTSFF 193
              + +   T  +  DP++MTYEEL +LG+SVG   +GLS+E+IS L T +   K  S++
Sbjct: 161 SPSSISHNPTDDENNDPENMTYEELTELGDSVGDVGKGLSQERISRLRTHKYGTKTKSWY 220

Query: 194 ---SRKKL---DERCVICQMRYKRGDKQIKLPCKHFYHNKCITKWLTINKICPICNIEVF 243
               +KK    D +C IC M Y +GDK   LPCKH YH  CI++WL  NK+C IC  EV+
Sbjct: 221 CLHMKKKFVADDSQCSICLMEYAKGDKITTLPCKHIYHKDCISQWLKQNKVCCICKAEVY 279

[Close]

BLAST of CSPI07G19300 vs. TAIR 10
Match: AT3G47180.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.8e-20
Identity = 54/128 (42.19%), Postives = 79/128 (61.72%), Query Frame = 0


Query: 112 VEEDVVDAHSIPEECDLSHREETSTSQVTWQDEIDPDHMTYEELLDLGESVGTESRGLSE 171
           V+ED ++     +E + +  EE    +   +DEIDPD ++YEEL+ LG+ +G E+RGL+ 
Sbjct: 81  VDEDEINEFLEDQESNSNLEEEDDFLEE--EDEIDPDQLSYEELIALGDFIGVENRGLTP 140

Query: 172 EQISLLPTARCKLTSFFSRKKLD-ERCVICQMRYKRGDKQIKL-PCKHFYHNKCITKWLT 231
            +IS    A    T  FS  K + +RCV+CQM ++  +  + L PC H YH++CITKWL 
Sbjct: 141 IEISTCLNAS---TYVFSHNKNEIDRCVVCQMEFEERESLVVLRPCDHPYHSECITKWLE 200

Query: 232 INKICPIC 238
             KICPIC
Sbjct: 201 TKKICPIC 203

The following BLAST results are available for this feature:

BLAST of CSPI07G19300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L649	4.6e-63	49.40	E3 ubiquitin-protein ligase BIG BROTHER OS=Arabidopsis thaliana OX=3702 GN=BB PE...	[more]
Q9LT17	1.9e-24	35.04	E3 ubiquitin ligase BIG BROTHER-related OS=Arabidopsis thaliana OX=3702 GN=BBR P...	[more]
Q9ZQF9	1.5e-13	43.43	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
O49500	2.6e-13	40.86	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q7XTV7	4.4e-13	42.55	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of CSPI07G19300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CED1	7.5e-149	99.61	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A5D3CGQ8	3.7e-148	99.21	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A0A0K8C4	1.0e-145	100.00	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G428220 P...	[more]
A0A1S3CES7	3.0e-145	99.60	E3 ubiquitin ligase BIG BROTHER isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034999...	[more]
A0A5A7UTY6	3.2e-139	94.53	E3 ubiquitin ligase BIG BROTHER isoform X1 OS=Cucumis melo var. makuwa OX=119469...	[more]

BLAST of CSPI07G19300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011659453.1	5.3e-149	100.00	E3 ubiquitin-protein ligase BIG BROTHER [Cucumis sativus] >XP_011659454.1 E3 ubi...	[more]
XP_008461282.1	1.6e-148	99.61	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo] >XP_0084612...	[more]
TYK10442.1	7.7e-148	99.21	E3 ubiquitin ligase BIG BROTHER isoform X1 [Cucumis melo var. makuwa]	[more]
XP_008461284.1	6.1e-145	99.60	PREDICTED: E3 ubiquitin ligase BIG BROTHER isoform X2 [Cucumis melo]	[more]
XP_038899262.1	9.1e-141	94.49	E3 ubiquitin-protein ligase BIG BROTHER [Benincasa hispida] >XP_038899263.1 E3 u...	[more]

BLAST of CSPI07G19300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63530.1	3.3e-64	49.40	RING/U-box superfamily protein	[more]
AT3G63530.2	3.3e-64	49.40	RING/U-box superfamily protein	[more]
AT3G19910.1	1.4e-25	35.04	RING/U-box superfamily protein	[more]
AT5G52140.1	5.9e-21	35.00	RING/U-box superfamily protein	[more]
AT3G47180.1	3.8e-20	42.19	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 197..237 e-value: 1.7E-4 score: 30.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 196..238 e-value: 1.5E-11 score: 44.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 197..238 score: 11.456568
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 159..242 e-value: 1.2E-17 score: 65.3
None	No IPR available	PANTHER	PTHR46400:SF3	E3 UBIQUITIN-PROTEIN LIGASE BIG BROTHER	coord: 1..247
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 196..242
IPR033276	E3 ubiquitin-protein ligase BIG BROTHER	PANTHER	PTHR46400	RING/U-BOX SUPERFAMILY PROTEIN	coord: 1..247

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G19300.1	CSPI07G19300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048437	floral organ development
biological_process	GO:0008285	negative regulation of cell population proliferation
biological_process	GO:0046621	negative regulation of organ growth
biological_process	GO:1900057	positive regulation of leaf senescence
biological_process	GO:0051865	protein autoubiquitination
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity